Comparing PP_2179 FitnessBrowser__Putida:PP_2179 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
7d50B Spua mutant - h221n with glutamyl-thioester (see paper)
69% identity, 97% coverage: 1:250/257 of query aligns to 6:255/255 of 7d50B
7d53A Spua mutant - h221n with glu (see paper)
69% identity, 96% coverage: 3:250/257 of query aligns to 2:249/249 of 7d53A
7d4rB Spua native structure (see paper)
61% identity, 95% coverage: 4:247/257 of query aligns to 1:214/215 of 7d4rB
6vtvB Crystal structure of puud gamma-glutamyl-gamma-aminobutyrate hydrolase from e. Coli
44% identity, 97% coverage: 5:253/257 of query aligns to 6:252/252 of 6vtvB
P76038 Gamma-glutamyl-gamma-aminobutyrate hydrolase PuuD; Gamma-Glu-GABA hydrolase; EC 3.5.1.94 from Escherichia coli (strain K12) (see paper)
44% identity, 97% coverage: 5:253/257 of query aligns to 8:254/254 of P76038
3fijA Crystal structure of a uncharacterized protein lin1909
35% identity, 91% coverage: 5:239/257 of query aligns to 3:216/224 of 3fijA
O33341 Putative glutamine amidotransferase Rv2859c; EC 2.4.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 93% coverage: 5:243/257 of query aligns to 66:295/308 of O33341
P49915 GMP synthase [glutamine-hydrolyzing]; GMP synthetase; Glutamine amidotransferase; EC 6.3.5.2 from Homo sapiens (Human) (see paper)
35% identity, 50% coverage: 101:229/257 of query aligns to 92:198/693 of P49915
Sites not aligning to the query:
>PP_2179 FitnessBrowser__Putida:PP_2179
MPRVPVIGITACTSMIELHATQTISEKYARAAAKAACGLPIVIPSLGELMDSADILDVVD
GLIFTGSPSNIEPYHYNGPASAAGTHHDQLRDATTLPLMRAAIAAGVPVLGICRGFQEMN
VALGGTLHQKVHETGVFMDHREGKDEPLDKQYGPRHTLHVEPGGMLDRMGLPAIFDVNSI
HGQGIDVLAPGLRVEALAPDGLVEAISVESSKGFALGVQWHPEFQVMDNPHYLAIFQSFG
KACRHRSVLRQRLLLSA
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory