SitesBLAST

P13650 Quinoprotein glucose dehydrogenase B; Glucose dehydrogenase B [pyrroloquinoline-quinone]; Soluble glucose dehydrogenase; s-GDH; EC 1.1.5.2 from Acinetobacter calcoaceticus (see paper)
27% identity, 97% coverage: 1:368/381 of query aligns to 11:448/478 of P13650

query
sites
P13650

M

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A

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-

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Q100 (= Q92) binding
D167 (≠ N156) binding
Q192 (vs. gap) binding
R252 (= R224) binding
G271 (= G244) binding
P272 (= P245) binding
E277 (= E250) binding
Y287 (= Y260) binding
A293 (vs. gap) binding
Y295 (vs. gap) binding
D297 (vs. gap) binding
E333 (≠ A279) binding
Y367 (≠ F305) binding
T372 (= T310) binding
K401 (≠ A324) binding

5minB Apo form of the soluble pqq-dependent glucose dehydrogenase from acinetobacter calcoaceticus
27% identity, 86% coverage: 40:368/381 of query aligns to 22:424/453 of 5minB

query
sites
5minB

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D

active site: H144 (= H157), D163 (≠ E176), R228 (= R224), A269 (vs. gap), Y271 (vs. gap), D273 (vs. gap), E309 (≠ A279)
binding calcium ion: E253 (= E250), Y263 (= Y260), A269 (vs. gap), Y271 (vs. gap), D273 (vs. gap), E309 (≠ A279)

1cruA Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqq and methylhydrazine (see paper)
26% identity, 86% coverage: 40:368/381 of query aligns to 22:422/448 of 1cruA

query
sites
1cruA

L

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D

D

active site: H142 (= H157), D161 (≠ E176), R226 (= R224), A267 (vs. gap), Y269 (vs. gap), D271 (vs. gap), E307 (≠ A279)
binding calcium ion: G245 (= G244), P246 (= P245), E251 (= E250), Y261 (= Y260), A267 (vs. gap), Y269 (vs. gap), D271 (vs. gap), E307 (≠ A279)
binding methylhydrazine: Y341 (≠ F305), W344 (≠ S308)
binding pyrroloquinoline quinone: Q76 (= Q92), H142 (= H157), R226 (= R224), N227 (= N225), Q244 (≠ H243), Y341 (≠ F305), T346 (= T310), A348 (vs. gap), L374 (= L323), K375 (≠ A324), R404 (= R351), R406 (= R353)

1cq1A Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqqh2 and glucose (see paper)
27% identity, 86% coverage: 40:368/381 of query aligns to 22:418/444 of 1cq1A

query
sites
1cq1A

L

I

A

L

D

S

G

N

L

L

R

N

N

K

P

P

W

H

A

A

L

L

A

L

F

W

L

G

P

P

G

D

G

N

K

Q

D

I

M

W

L

L

V

T

T

E

E

R

R

A

A

T

G

G

N

K

L

I

R

L

L

R

V

V

N

N

A

P

E

E

G

S

K

G

V

S

G

V

P

K

S

T

I

V

S

F

G

Q

V

V

P

P

K

E

V

I

W

V

-

N

-

D

A

A

E

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

S

H

P

P

E

D

F

F

G

K

K

N

D

N

R

P

T

Y

V

I

Y

Y

L

I

S

S

Y

G

A

T

E

F

E

K

G

N

S

P

D

K

G

P

K

N

A

Q

G

-

T

T

A

I

V

I

G

R

R

R

G

Y

Q

T

L

Y

S

N

E

K

D

S

R

T

A

D

R

T

L

L

E

E

N

K

F

P

T

V

V

D

I

L

F

L

R

A

Q

G

Q

L

P

P

K

-

L

-

S

S

V

S

G

K

N
x
D

H
|
H

F

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

D

D

G

Q

Y

K

L

I

F

Y

I

Y

A

T

L

I

G

G

E
x
D

N

Q

-

G

-

R

-

N

-
x
Q

-

L

-

A

-

Y

-

L

-

F

-

L

-

P

N

N

Q

Q

-

A

-

Q

-

H

R

T

P

P

T

T

A

Q

Q

Q

D

E

L

L

D

N

-

G

-

K

-

D

-

Y

-

H

K

T

L

Y

Q

M

G

G

K

K

V

V

V

L

R

R

I

L

L

N

P

L

D

D

G

G

E

S

V

I

P

P

K

K

D

D

N

N

P

P

F

S

V

F

G

-

K

-

D

N

N

G

V

V

R

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N
|
N

Q

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

W

-

T

N

G

G

Q

K

L

L

W

L

T

Q

H

S

E

E

H
x
Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

I

I

N

N

I

L

P

I

K

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

-

N

-

V

-
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

-

K

-

D

I

L

A

A

T

Q

H

N

G

G

I

V

N

K

Y

V

S

A

L

A

-

G

L

V

P

P

I

V

P

T

E

K

A
x
E

K

S

G

E

E

W

H

T

V

G

D

K

G

N

M

F

V

V

D

P

P

P

-

L

-

K

-

T

-

L

H

Y

H

T

V

V

W

Q

E

D

-

T

-

Y

-

N

-

Y

K

N

S

D

P

P

G

T

I

C

S

G

G

E

M

M

A

T

F
x
Y

Y

I

D

C

S

W

P

P

T
|
T

-

V

-
x
A

-

P

-

S

-

A

-

Y

-

V

-

Y

-

K

-

G

-

K

-

A

F

I

K

T

A

G

W

W

D

E

H

N

N

T

L

L

F

L

I

V

G

P

A

S

L
|
L

A
x
K

T

R

Q

G

E

V

L

I

I

F

R

R

L

I

Q

K

L

L

D

D

G

P

D

T

K

Y

V

S

V

T

H

T

E

Y

E

D

R

D

L

A

L

V

G

P

D

M

L

F

K

K

A

S

-

N

R
|
R

I

Y

R
|
R

D

D

V

V

R

I

V

A

G

S

P

P

D

D

G

G

-

N

Y

V

L

L

Y

Y

V

V

L

L

T

T

D

D

active site: H138 (= H157), D157 (≠ E176), R222 (= R224), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A279)
binding beta-D-glucopyranose: D137 (≠ N156), H138 (= H157), Q162 (vs. gap), Y337 (≠ F305)
binding calcium ion: G241 (= G244), P242 (= P245), E247 (= E250), Y257 (= Y260), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A279)
binding pyrroloquinoline quinone: Q76 (= Q92), H138 (= H157), R222 (= R224), N223 (= N225), Q240 (≠ H243), Y337 (≠ F305), T342 (= T310), A344 (vs. gap), L370 (= L323), K371 (≠ A324), R400 (= R351), R402 (= R353)

1c9uA Crystal structure of the soluble quinoprotein glucose dehydrogenase in complex with pqq (see paper)
27% identity, 86% coverage: 40:368/381 of query aligns to 22:418/444 of 1c9uA

query
sites
1c9uA

L

I

A

L

D

S

G

N

L

L

R

N

N

K

P

P

W

H

A

A

L

L

A

L

F

W

L

G

P

P

G

D

G

N

K

Q

D

I

M

W

L

L

V

T

T

E

E

R

R

A

A

T

G

G

N

K

L

I

R

L

L

R

V

V

N

N

A

P

E

E

G

S

K

G

V

S

G

V

P

K

S

T

I

V

S

F

G

Q

V

V

P

P

K

E

V

I

W

V

-

N

-

D

A

A

E

D

G

G

Q
|
Q

G

N

G

G

L

L

D

G

V

F

A

A

L

F

S

H

P

P

E

D

F

F

G

K

K

N

D

N

R

P

T

Y

V

I

Y

Y

L

I

S

S

Y

G

A

T

E

F

E

K

G

N

S

P

D

K

G

P

K

N

A

Q

G

-

T

T

A

I

V

I

G

R

R

R

G

Y

Q

T

L

Y

S

N

E

K

D

S

R

T

A

D

R

T

L

L

E

E

N

K

F

P

T

V

V

D

I

L

F

L

R

A

Q

G

Q

L

P

P

K

-

L

-

S

S

V

S

G

K

N

D

H
|
H

F

Q

G

S

S

G

R

R

L

L

V

V

F

I

D

G

R

P

D

D

G

Q

Y

K

L

I

F

Y

I

Y

A

T

L

I

G

G

E
x
D

N

Q

-

G

-

R

-

N

-

Q

-

L

-

A

-

Y

-

L

-

F

-

L

-

P

N

N

Q

Q

-

A

-

Q

-

H

R

T

P

P

T

T

A

Q

Q

Q

D

E

L

L

D

N

-

G

-

K

-

D

-

Y

-

H

K

T

L

Y

Q

M

G

G

K

K

V

V

V

L

R

R

I

L

L

N

P

L

D

D

G

G

E

S

V

I

P

P

K

K

D

D

N

N

P

P

F

S

V

F

G

-

K

-

D

N

N

G

V

V

R

V

P

S

E

H

I

I

W

Y

S

T

Y

L

G

G

H

H

R
|
R

N
|
N

Q

P

Q

Q

G

G

A

L

A

A

L

F

N

T

P

P

W

-

T

N

G

G

Q

K

L

L

W

L

T

Q

H

S

E

E

H
x
Q

G
|
G

P
|
P

R

N

G

S

G

D

D

D

E
|
E

I

I

N

N

I

L

P

I

K

V

P

K

G

G

K

G

N

N

Y
|
Y

G

G

W

W

P

P

-

N

-

V

-
x
A

-

G

-
x
Y

-

K

-
x
D

-

D

-

S

-

G

-

Y

-

A

-

Y

-

A

-

N

-

Y

-

S

-

A

-

N

-

K

-

S

-

I

-

K

-

D

I

L

A

A

T

Q

H

N

G

G

I

V

N

K

Y

V

S

A

L

A

-

G

L

V

P

P

I

V

P

T

E

K

A
x
E

K

S

G

E

E

W

H

T

V

G

D

K

G

N

M

F

V

V

D

P

P

P

-

L

-

K

-

T

-

L

H

Y

H

T

V

V

W

Q

E

D

-

T

-

Y

-

N

-

Y

K

N

S

D

P

P

G

T

I

C

S

G

G

E

M

M

A

T

F
x
Y

Y

I

D

C

S
x
W

P

P

T
|
T

-

V

-

A

-

P

-

S

-

A

-

Y

-

V

-

Y

-

K

-

G

-

K

-

A

F

I

K

T

A

G

W

W

D

E

H

N

N

T

L

L

F

L

I

V

G

P

A

S

L
|
L

A
x
K

T

R

Q

G

E

V

L

I

I

F

R

R

L

I

Q

K

L

L

D

D

G

P

D

T

K

Y

V

S

V

T

H

T

E

Y

E

D

R

D

L

A

L

V

G

P

D

M

L

F

K

K

A

S

-

N

R
|
R

I

Y

R
|
R

D

D

V

V

R

I

V

A

G

S

P

P

D

D

G

G

-

N

Y

V

L

L

Y

Y

V

V

L

L

T

T

D

D

active site: H138 (= H157), D157 (≠ E176), R222 (= R224), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A279)
binding calcium ion: R222 (= R224), G241 (= G244), P242 (= P245), E247 (= E250), Y257 (= Y260), A263 (vs. gap), Y265 (vs. gap), D267 (vs. gap), E303 (≠ A279)
binding pyrroloquinoline quinone: Q76 (= Q92), H138 (= H157), R222 (= R224), N223 (= N225), Q240 (≠ H243), Y337 (≠ F305), W340 (≠ S308), T342 (= T310), L370 (= L323), K371 (≠ A324), R400 (= R351), R402 (= R353)

7pgmB Hhip-c in complex with heparin (see paper)
25% identity, 50% coverage: 34:222/381 of query aligns to 3:203/427 of 7pgmB

query
sites
7pgmB

T

C

L

F

I

C

V

I

D

Q

T

E

L

V

A

V

D

S

G

G

L

L

R

R

N

Q

P

P

W

V

-

G

A

A

L

L

A

H

F

S

L

G

P

D

G

G

G

S

K

Q

D

R

M

L

L

F

V

I

T

L

E

E

R

K

A

E

G

G

N

Y

L

V

R

K

L

I

V

L

N

T

A

P

E

E

G

G

K

E

V

I

-

F

-

K

G

E

P

P

S

Y

I

L

S

D

G

-

V

I

P

H

K

K

V

L

W

V

A

Q

E

S

G

G

-

I

-
x
K

-
x
G

-

G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

S

H

P

P

E

N

F

Y

G

K

K

K

D

N

R

G

T

K

V

L

Y

Y

L

V

S

S

Y

Y

A

T

E

T

E

N

G

Q

S

H

D

D

G

H

-

I

-

L

-

R

K

V

A

V

G

E

T

Y

A

T

V

V

G

S

R

R

G

K

Q

N

L

P

S

H

E

Q

D

V

R

D

A

L

R

R

L

T

E

A

N

R

F

-

T

-

V

-

I

V

F

F

R

L

Q

E

Q

V

P

A

K

E

L

L

S

H

V

R

G

-

N
x
K

H

H

F

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

D

D

G

G

Y

F

L

L

F

Y

I

I

A

I

L

L

G

G

E

D

N

G

N

-

Q

-

R

M

P

I

T

T

A

L

Q

D

D

D

L

M

D

E

K

S

-

D

L

F

Q

T

G

G

K

S

V

V

V

L

R

R

I

L

L

D

P

V

D

D

G

T

E

D

-

M

-

C

-

N

-

V

-

P

-

Y

-

S

V

I

P

P

K

R

D

S

N

N

P

P

F

H

V

F

G

N

K

S

D

T

N

N

V

Q

R

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding 2-O-sulfo-alpha-L-idopyranuronic acid: K64 (vs. gap), G65 (vs. gap), K131 (≠ N156)
binding 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose: K64 (vs. gap), G65 (vs. gap)

Sites not aligning to the query:

binding 2-deoxy-6-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose: 326

7pgnB Hhp-c in complex with glycosaminoglycan mimic sos (see paper)
24% identity, 50% coverage: 34:222/381 of query aligns to 3:208/438 of 7pgnB

query
sites
7pgnB

T

C

L

F

I

C

V

I

D

Q

T

E

L

V

A

V

D

S

G

G

L

L

R

R

N

Q

P

P

W

V

-

G

A

A

L

L

A

H

F

S

L

G

P

D

G

G

G

S

K

Q

D

R

M

L

L

F

V

I

T

L

E

E

R

K

A

E

G

G

N

Y

L

V

R

K

L

I

V

L

N

T

A

P

E

E

G

G

K

E

V

I

-

F

-

K

G

E

P

P

S

Y

I

L

S

D

G

-

V

I

P

H

K

K

V

L

W

V

A

Q

E

S

G

G

-

I

-
x
K

-
x
G

-

G

-

D

Q
x
E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

S

H

P

P

E

N

F

Y

G

K

K

K

D

N

R

G

T

K

V

L

Y

Y

L

V

S

S

Y

Y

A

T

E

T

E

N

G

Q

S

H

D

D

G

H

-

I

-

L

-

R

K

V

A

V

G

E

T

Y

A

T

V

V

G

S

R

R

G

K

Q

N

L

P

S

H

E

Q

D

V

R

D

A

L

R

R

L

T

E

A

N

R

F

-

T

-

V

-

I

V

F

F

R

L

Q

E

Q

V

P

A

K

E

L

L

S

H

V
x
R

G

-

N
x
K

H

H

F

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

D

D

G

G

Y

F

L

L

F

Y

I

I

A

I

L

L

G

G

E

D

N

G

-

M

-

I

-

T

-

L

N

D

Q

D

R

M

P

E

T

E

A

M

Q

D

D

G

L

L

D

S

K

D

L

F

Q

T

G

G

K

S

V

V

V

L

R

R

I

L

L

D

P

V

D

D

G

T

E

D

-

M

-

C

-

N

-

V

-

P

-

Y

-

S

V

I

P

P

K

R

D

S

N

N

P

P

F

H

V

F

G

N

K

S

D

T

N

N

V

Q

R

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding calcium ion: E68 (≠ Q92)
binding 2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose: K64 (vs. gap), K64 (vs. gap), G65 (vs. gap), R130 (≠ V154), K131 (≠ N156)

Sites not aligning to the query:

7pgnA Hhp-c in complex with glycosaminoglycan mimic sos (see paper)
24% identity, 50% coverage: 31:222/381 of query aligns to 1:209/437 of 7pgnA

query
sites
7pgnA

E

K

E

H

G

N

T

C

L

F

I

C

V

I

D

Q

T

E

L

V

A

V

D

S

G

G

L

L

R

R

N

Q

P

P

W

V

-

G

A

A

L

L

A

H

F

S

L

G

P

D

G

G

G

S

K

Q

D

R

M

L

L

F

V

I

T

L

E

E

R

K

A

E

G

G

N

Y

L

V

R

K

L

I

V

L

N

T

A

P

E

E

G

G

K

E

V

I

-

F

-

K

G

E

P

P

S

Y

I

L

S

D

G

-

V

I

P

H

K

K

V

L

W

V

A

Q

E

S

G

G

-

I

-
x
K

-
x
G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

S

H

P

P

E

N

F

Y

G

K

K

K

D

N

R

G

T

K

V

L

Y

Y

L

V

S

S

Y

Y

A

T

E

T

E

N

G

Q

S

H

D

D

G

H

-

I

-

L

-

R

K

V

A

V

G

E

T

Y

A

T

V

V

G

S

R

R

G

K

Q

N

L

P

S

H

E

Q

D

V

R

D

A

L

R

R

L

T

E

A

N

R

F

-

T

-

V

-

I

V

F

F

R

L

Q

E

Q

V

P

A

K

E

L

L

S

H

V

R

G

-

N
x
K

H

H

F

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

D

D

G

G

Y

F

L

L

F

Y

I

I

A

I

L

L

G

G

E

D

N

G

-

M

-

I

-

T

-

L

N

D

Q

D

R

M

P

E

T

E

A

M

Q

D

D

G

L

L

D

S

K

D

L

F

Q

T

G

G

K

S

V

V

V

L

R

R

I

L

L

D

P

V

D

D

G

T

E

D

-

M

-

C

-

N

-

V

-

P

-

Y

-

S

V

I

P

P

K

R

D

S

N

N

P

P

F

H

V

F

G

N

K

S

D

T

N

N

V

Q

R

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

binding 1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose: K65 (vs. gap), G66 (vs. gap), G67 (vs. gap), K132 (≠ N156)

Sites not aligning to the query:

binding 2,3,4,6-tetra-O-sulfonato-alpha-D-glucopyranose: 283, 338

Q96QV1 Hedgehog-interacting protein; HHIP; HIP from Homo sapiens (Human) (see 3 papers)
24% identity, 50% coverage: 31:222/381 of query aligns to 213:428/700 of Q96QV1

query
sites
Q96QV1

E

K

E

H

G

N

T

C

L

F

I

C

V

I

D

Q

T

E

L

V

A

V

D

S

G

G

L

L

R

R

N

Q

P

P

W

V

-

G

A

A

L

L

A

H

F

S

L

G

P

D

G

G

G

S

K

Q

D

R

M

L

L

F

V

I

T

L

E

E

R

K

A

E

G

G

N

Y

L

V

R

K

L

I

V

L

N

T

A

P

E

E

G

G

K

E

V

I

-

F

-

K

G

E

P

P

S

Y

I

L

S

D

G

-

V

I

P

H

K

K

V

L

W

V

A

Q

E

S

G

G

-

I

-

K

-

G

-

D

Q

E

G

R

G

G

L

L

D

S

V

L

A

A

L

F

S

H

P

P

E

N

F

Y

G

K

K

K

D

N

R

G

T

K

V

L

Y

Y

L

V

S

S

Y

Y

A

T

E

T

E

N

G

Q

S

E

D

R

G

W

K

A

A

I

G

G

T

P

A

-

V

-

G

-

R

-

G

-

Q

-

L

-

S

H

E

D

D

H

R

I

A

L

R

R

L

V

E

V

N

E

F

Y

T

T

V

V

I

S

F

-

R

R

Q

K

Q

N

P

P

K

H

-

Q

-

V

-

D

-

L

-

R

-

T

-

A

-

R

-
x
V

-

F

L

L

S

E

V

V

G

A

-

E

-

L

-

H

-

R

N

K

H

H

F

L

G

G

S

G

R

Q

L

L

V

L

F

F

D

G

R

P

D

D

G

G

Y

F

L

L

F

Y

I

I

A

I

L

L

G

G

E

D

N

G

-

M

-

I

-

T

-

L

N

D

Q

D

R

M

P
x
E

T

E

A
x
M

Q
x
D

D

G

L

L

D

S

K
x
D

L

F

Q

T

G

G

K

S

V

V

V

L

R

R

I

L

L

D

P

V

D

D

G

T

E

D

-

M

-

C

-

N

-

V

-

P

-

Y

-

S

V

I

P

P

K

R

D

S

N

N

P

P

F

H

V

F

G

N

K

S

D

T

N

N

V

Q

R

P

P

P

E

E

I

V

W

F

S

A

Y

H

G

G

V341 (vs. gap) natural variant: V -> I
E380 (≠ P181) mutation to A: Abolishes SHH binding.
M382 (≠ A183) mutation to A: Abolishes SHH binding.
D383 (≠ Q184) mutation D->A,R: Abolishes SHH binding.
D387 (≠ K188) mutation to A: Abolishes SHH binding.

Sites not aligning to the query:

447 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Query Sequence

>PP_2198 PP_2198 aldose sugar dehydrogenase
MLRAHWLATLTAAALLPLWAHAAAEQRFPSEEGTLIVDTLADGLRNPWALAFLPGGKDML
VTERAGNLRLVNAEGKVGPSISGVPKVWAEGQGGLLDVALSPEFGKDRTVYLSYAEEGSD
GKAGTAVGRGQLSEDRARLENFTVIFRQQPKLSVGNHFGSRLVFDRDGYLFIALGENNQR
PTAQDLDKLQGKVVRILPDGEVPKDNPFVGKDNVRPEIWSYGHRNQQGAALNPWTGQLWT
HEHGPRGGDEINIPKPGKNYGWPIATHGINYSLLPIPEAKGEHVDGMVDPHHVWEKSPGI
SGMAFYDSPTFKAWDHNLFIGALATQELIRLQLDGDKVVHEERLLGDLKARIRDVRVGPD
GYLYVLTDDKDGALLKVGLAD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory