SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_2263 FitnessBrowser__Putida:PP_2263 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
27% identity, 98% coverage: 7:266/266 of query aligns to 13:279/281 of P94530

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P94530

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D180 (= D168) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
26% identity, 76% coverage: 65:266/266 of query aligns to 87:294/296 of P68183

query
sites
P68183

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E190 (= E163) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A165) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G169) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P182) mutation to A: No effect.

Query Sequence

>PP_2263 FitnessBrowser__Putida:PP_2263
MSTRKSMALLLYFFFLLVPIYWLLNMSFKSNAEILGGLTLWPQAFTLDNYRVIFTDASWY
SGYINSLYYVCLNTLISLLVALPAAYAFSRYRFLGDRHLFFWLLTNRMAPPAVFLLPFFQ
LYSSIGLFDTHIAVALAHCLFNVPLAVWILEGFMSGVPREIDETAYIDGYSFPRFFVKIF
IPLIGSGIGVTAFFCFMFSWVELLLARTLTSVNAKPIAAVMTRTVSASGIDWGVLAAAGV
LTILPGMLVIWFVRNHVAKGFALGRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory