SitesBLAST

V174 (≠ A209) binding
H244 (≠ Y277) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (≠ F341) binding
327:333 (vs. 364:370, 14% identical) binding
R329 (≠ G366) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
22% identity, 62% coverage: 124:386/427 of query aligns to 92:349/364 of 1ng3A

query
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binding acetylamino-acetic acid: Y241 (≠ L273), Y246 (= Y288), R302 (≠ F341), R329 (≠ G366)
binding flavin-adenine dinucleotide: P173 (= P208), V174 (≠ A209), A201 (= A235), S202 (≠ G236), G203 (≠ N237), W205 (≠ Y239), F209 (≠ L243), G224 (≠ T256), Y246 (= Y288), G300 (= G339), R302 (≠ F341), H327 (≠ C364), F328 (≠ S365), R329 (≠ G366), N330 (≠ H367), G331 (= G368), I332 (≠ V369), L333 (≠ T370)
binding phosphate ion: R254 (= R296)

Sites not aligning to the query:

active site: 47, 48, 49
binding acetylamino-acetic acid: 49
binding flavin-adenine dinucleotide: 10, 11, 12, 13, 14, 15, 33, 34, 35, 41, 42, 43, 45, 46, 47, 48, 49
binding phosphate ion: 89

S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
25% identity, 50% coverage: 182:393/427 of query aligns to 147:359/369 of S5FMM4

query
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S5FMM4

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S202 (≠ G236) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ V369) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ L379) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
22% identity, 62% coverage: 124:386/427 of query aligns to 92:349/364 of 3if9A

query
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3if9A

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binding flavin-adenine dinucleotide: P173 (= P208), V174 (≠ A209), A201 (= A235), S202 (≠ G236), G203 (≠ N237), W205 (≠ Y239), F209 (≠ L243), G224 (≠ T256), Y246 (= Y288), G300 (= G339), R302 (≠ F341), P303 (≠ L342), H327 (≠ C364), F328 (≠ S365), R329 (≠ G366), N330 (≠ H367), G331 (= G368), I332 (≠ V369), L333 (≠ T370)
binding glycolic acid: Y246 (= Y288), R302 (≠ F341), R329 (≠ G366)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 10, 11, 12, 13, 14, 15, 33, 34, 35, 41, 42, 43, 45, 46, 47, 48, 49

5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
23% identity, 85% coverage: 29:391/427 of query aligns to 24:374/383 of 5i39A

query
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5i39A

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P

-

K

K

M

L

L

K

K

A

A

E

F

F

P

P

Q

A

L

F

K

K

N

E

V

S

K

K

I

L

D

I

Y

D

A

Q

W

W

T

S

G
|
G

N
x
A

F
x
M

L

A

L

I

T

A

L

P

S

D

R

E

L

N

P

P

Q

I

V

I

G

S

R

E

I

V

G

K

D

E

N

Y

I

P

-

G

Y

L

Y

V

S

I

Q

N

G

T

C

A

S
x
T

G
|
G

H
x
W

G
|
G

V
x
M

T
|
T

Y

E

T

S

H

P

L

V

A

S

G

A

K

E

V

L

L

T

A

A

E

D

A

L

L

L

R

L

G

G

Q

K

A

K

E

P

R

V

F

L

D

D

active site: F66 (≠ N71), Q69 (= Q74), A70 (≠ I75), Q248 (≠ S251), P267 (= P274)
binding flavin-adenine dinucleotide: V30 (≠ I35), G31 (= G36), G33 (= G38), I34 (≠ Y39), L35 (≠ T40), V53 (= V58), E54 (= E59), K55 (≠ A60), E61 (≠ G66), Q62 (≠ A67), S63 (= S68), R65 (= R70), F66 (≠ N71), Y67 (≠ G72), G68 (= G73), Q69 (= Q74), A196 (≠ P208), A197 (= A209), A225 (= A235), G226 (= G236), G227 (≠ N237), W229 (vs. gap), F233 (≠ Y239), Q248 (≠ S251), Q250 (≠ T256), G321 (= G339), A322 (≠ N340), M323 (≠ F341), T348 (≠ S365), G349 (= G366), W350 (≠ H367), G351 (= G368), M352 (≠ V369), T353 (= T370)

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
21% identity, 79% coverage: 48:383/427 of query aligns to 23:351/374 of 1y56B

query
sites
1y56B

L

L

L

A

E

K

N

R

G

G

F

E

K

E

V

V

S

T

I

V

V
x
I

E
|
E

A
x
K

A

R

K

F

V

I

G

G

F
x
S

G
|
G

A
x
S

S
x
T

G
x
F

R
|
R

N
x
C

G
|
G

G
x
T

Q
x
G

I

I

V

R

N

Q

S

Q

Y

F

S

N

R

D

D

E

I

A

D

N

V

V

I

-

E

-

R

-

T

R

V

V

G

M

P

K

K

R

Q

S

A

V

Q

E

L

L

L

W

G

K

H

K

M

Y

A

S

F

E

E

E

G

Y

G

G

R

F

I

S

I

F

R

K

E

Q

R

-

V

-

A

-

K

-

Y

-

N

-

I

-

Q

-

C

-

D

-

L

-

K

-

D

T

G

G

G

Y

V

L

F

F

A

L

L

Y

T

D

S

D

K

E

Q

E

M

V

G

K

H

T

L

F

E

K

S

R

Q

N

K

I

R

E

L

I

W

Q

E

N

R

K

F

F

G

G

H

V

N

-

Q

P

L

T

E

K

L

L

M

I

D

T

Q

P

K

E

R

E

I

A

R

K

E

E

V

I

V

V

A

P

-

L

-

D

C

I

D

S

S

E

Y

V

I

I

G

A

M

S

L

W

D

N

M

P

S

T

G

D

G

G

H

K

I

A

H

D

P

P

L

F

N

E

L

A

A

T

L

T

G

A

E

F

A

A

A

V

A

K

V

A

E

K

S

E

L

Y

G

G

G

A

I

K

I

L

-

L

-

E

Y

Y

E

T

Q
x
E

T
x
V

P

K

A

G

V

F

R

L

I

I

E

E

R

N

G

N

A

E

N

I

P

K

V

G

V

V

H

K

T

T

P

N

Q

K

G

G

K

I

V

I

R

K

A

T

K

G

F

I

I

V

V

N

A
|
A

G
x
T

N
|
N

A

A

Y
x
W

L

-

G

A

N

N

L

L

V
x
I

P

N

E

A

L

M

A

A

A

G

K

I

S

K

M

T

-

K

-

I

-

P

-

I

-

E

P

P

C

Y

G

K

T
x
H

Q

Q

V

A

I

V

T
x
I

T

T

E

Q

P

P

L

I

G

K

E

R

E

-

L

-

A

-

R

G

T

T

L

I

L

N

P
|
P

Q

M

D

V

Y

I

C

S

V

F

E

K

D

Y

C

G

N

H

-

A

Y
|
Y

L

L

L

T

D

Q

Y

T

Y

F

R

H

L

G

T

G

S

I

D
x
I

K

G

R

G

L

I

I

G

F

Y

G

E

G

I

G

G

V

P

V

T

Y

Y

G

D

A

L

R

T

D

P

P

T

A

Y

N

E

I

F

E

L

A

R

I

E

I

V

R

S

P

Y

K

Y

M

F

L

T

K

K

A

I

F

I

P

P

Q

A

L

L

K

K

N

N

V

L

K

L

I

I

D

L

Y
x
R

A

T

W
|
W

T

A

G
|
G

N
x
Y

F
x
Y

L

A

L

K

T

T

L

P

S

D

R

S

L

N

P

P

Q

A

V

I

G

G

R

R

I

I

G

E

D

E

-

L

N

N

I

D

Y

Y

-

I

S

A

Q

A

G

G

C
x
F

S

S

G
|
G

H
|
H

G
|
G

V
x
F

T
x
M

Y

M

T

A

H

P

L

A

A

V

G

G

K

E

V

M

L

V

A

A

E

E

A

L

L

I

active site: F44 (≠ G69), G47 (= G72), T48 (≠ G73), H224 (≠ T256), P239 (= P274), G305 (= G339), M338 (≠ T370)
binding flavin-adenine dinucleotide: I33 (≠ V58), E34 (= E59), K35 (≠ A60), S40 (≠ F65), G41 (= G66), S42 (≠ A67), T43 (≠ S68), R45 (= R70), C46 (≠ N71), G47 (= G72), T48 (≠ G73), G49 (≠ Q74), E170 (≠ Q206), V171 (≠ T207), A199 (= A235), T200 (≠ G236), N201 (= N237), W203 (≠ Y239), I207 (≠ V244), Y250 (= Y284), G305 (= G339), Y306 (≠ N340), Y307 (≠ F341), F332 (≠ C364), G334 (= G366), H335 (= H367), G336 (= G368), F337 (≠ V369), M338 (≠ T370)
binding flavin mononucleotide: F44 (≠ G69), R45 (= R70), C46 (≠ N71), I228 (≠ T260), I260 (≠ D294), R301 (≠ Y335), W303 (= W337)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 10, 11, 13, 14, 15

Q9UI17 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Homo sapiens (Human) (see 4 papers)
22% identity, 61% coverage: 7:266/427 of query aligns to 26:280/866 of Q9UI17

query
sites
Q9UI17

P

P

Q

R

S

S

Y

V

Y

C

A

G

A

R

S

E

A

G

N

E

P

E

V

K

P

P

R

L

P

S

A

A

-

E

L

T

Q

Q

G

W

E

K

V

D

E

R

T

A

D

E

V

T

C

V

I

I

G

G

A

G

G

G

Y
x
C

T
x
V

G

G

L

V

S

S

S

L

A

A

L

Y

F

H

L

L

L

A

E

K

N

A

G

G

F

M

K

K

-

D

V

V

S

V

I

L

V

L

E
|
E

A
x
K

A

S

K

E

V

L

G

T

F

A

G
|
G

A
x
S

S
x
T

G
x
W

R
x
H

N
x
A

G
x
A

G
|
G

Q

-

I
x
L

V

T

N

T

S

Y

Y

F

S

H

R

P

D

G

I

I

D

N

V

L

I

-

E

-

R

K

T

K

V

I

G
x
H

P

Y

K

D

Q

S

A

I

Q

K

L

L

L

Y

G

E

H

K

M

L

A

E

F

E

E

E

G

T

G

G

R

Q

I

V

R

G

E

F

R

H

V

-

A

-

K

-

Y

-

N

-

I

-

Q

-

C

-

D

-

L

-

K

Q

D

P

G

G

G

S

V

I

F

R

A

L

A

A

L

T

T

T

S

P

K

V

Q

R

M

V

G

D

H

E

L

F

E

K

S

Y

Q

Q

K

M

R

-

L

-

W

-

E

T

R

R

F

T

G

G

H

W

N

H

Q

A

L

T

E

E

-

Q

-

Y

L

L

M

I

D

E

Q

P

K

E

R

K

I

I

R

Q

E

E

-

M

-

F

-

P

V

L

A

N

C

M

D

N

S

K

Y

V

I

L

G

A

G

G

M

L

L

Y

D

N

M

P

S

G

G

D

G

G

H

H

I

I

H

D

P

P

L

Y

N

S

L

L

A

T

L

M

G

A

E

L

A

A

A

G

V

A

E

R

S

K

L

C

G

G

G

A

I

L

Y

K

E

Y

Q

P

T

A

P

P

A
x
V

V

T

R

S

I

L

E

K

R

A

G

R

A

S

N

D

P

G

V

T

-

W

-

D

V

V

H

E

T

T

P

P

Q

Q

G

G

K

S

V

M

R

R

A

A

K

N

F

R

I

I

I

V

V

N

A

A

G

A

N

G

A

F

Y

W

-

A

-

R

-

E

L

V

G

G

N

K

L

M

V

I

P

-

E

G

L

L

A

E

A

H

K

P

S

L

M

I

P

P

C

V

G

Q

T

H

Q

Q

V

Y

I

V

T

V

T

T

E

S

P

T

L

I

G
x
S

E

E

CV 59:60 (≠ YT 39:40) binding
EK 80:81 (≠ EA 59:60) binding
87:95 (vs. 66:75, 20% identical) binding
H91 (≠ R70) modified: Tele-8alpha-FAD histidine
H109 (≠ G91) to R: in DMGDHD; shows 10 fold lower catalytic efficiency due to lower cofactor saturation and reduced thermal stability; dbSNP:rs121908331
V219 (≠ A209) binding
S279 (≠ G265) to P: in dbSNP:rs532964

Sites not aligning to the query:

397:402 binding
530 A → G: in dbSNP:rs1805073
646 S → P: in dbSNP:rs1805074

5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
25% identity, 49% coverage: 29:239/427 of query aligns to 16:221/433 of 5hxwA

query
sites
5hxwA

E

Q

T

A

D

D

V

V

C

V

I

V

I
x
V

G
|
G

A
|
A

G
|
G

Y
x
I

T
x
L

G

G

L

I

S

M

S

T

A

A

L

I

F

N

L

L

L

V

E

E

N

R

G

G

F

L

K

S

V

V

S

V

I

I

V
|
V

E
|
E

A
x
K

A

G

K

N

V

I

G

A

F

G

G
x
E

A
x
Q

S
|
S

G

S

R
|
R

N
x
F

G
x
Y

G
|
G

Q
|
Q

I
x
A

V

I

N

-

S

S

Y

Y

S

K

R

M

D

-

I

-

D

-

V

-

I

-

E

-

R

-

T

-

V

-

G

-

P

P

K

D

Q

E

A

T

Q

F

L

L

G

H

H

H

M

L

A

-

F

-

E

-

G

G

G

K

R

H

I

R

I

W

R

R

E

E

R

M

V

N

A

A

K

K

Y

V

N

G

I

I

Q

D

C

T

D

T

L

Y

K

R

D

T

G

Q

G

G

-

R

V

V

F

E

A

V

A

P

L

L

T

D

S

E

K

E

Q

D

M

L

G

V

H

N

L

V

E

-

S

-

Q

R

K

K

R

W

L

I

W

D

E

E

R

R

F

S

G

K

H

N

N

V

Q

G

L

S

E

D

L

I

M

P

D

F

Q

K

K

T

R

R

I

I

R

I

E

E

V

G

V

A

A

E

C

L

D

N

S

Q

Y

R

I

L

G

R

G

G

M

A

-

T

-

D

-

W

-

K

-

I

-

A

-

G

L

F

D

E

M

E

S

D

G

S

G

G

H

S

I
x
F

H

D

P

P

L

E

N

V

L

A

A

T

L

F

G

V

E

M

A

A

A

E

A

Y

V

A

E

K

S

K

L

M

G

G

G

V

I

R

I

I

Y

Y

E

T

Q

Q

T

C

P
x
A

A
|
A

V

R

R

G

I

L

E

E

R

T

G

Q

A

A

N

G

P

V

V

I

-

S

-

D

V

V

H

V

T

T

P

E

Q

K

G

G

K

A

V

I

R

K

A

T

K

S

F

Q

I

V

V

V

A
|
A

G
|
G

N
x
G

A

V

Y
x
W

active site: F58 (≠ N71), Q61 (= Q74), A62 (≠ I75)
binding flavin-adenine dinucleotide: V22 (≠ I35), G23 (= G36), A24 (= A37), G25 (= G38), I26 (≠ Y39), L27 (≠ T40), V45 (= V58), E46 (= E59), K47 (≠ A60), E53 (≠ G66), Q54 (≠ A67), S55 (= S68), R57 (= R70), F58 (≠ N71), Y59 (≠ G72), G60 (= G73), Q61 (= Q74), F163 (≠ I183), A188 (≠ P208), A189 (= A209), A217 (= A235), G218 (= G236), G219 (≠ N237), W221 (≠ Y239)

Sites not aligning to the query:

active site: 240, 284, 288
binding cetyl-trimethyl-ammonium: 236, 240, 279, 281, 291, 294, 303, 305, 307, 309, 310, 311, 313, 314, 314, 317, 318, 319, 320, 373, 375, 379, 399, 400
binding flavin-adenine dinucleotide: 240, 242, 331, 371, 372, 373, 398, 399, 400, 401, 402, 403

Query Sequence

>PP_2448 FitnessBrowser__Putida:PP_2448
MANTPYPQSYYAASANPVPPRPALQGEVETDVCIIGAGYTGLSSALFLLENGFKVSIVEA
AKVGFGASGRNGGQIVNSYSRDIDVIERTVGPKQAQLLGHMAFEGGRIIRERVAKYNIQC
DLKDGGVFAALTSKQMGHLESQKRLWERFGHNQLELMDQKRIREVVACDSYIGGMLDMSG
GHIHPLNLALGEAAAVESLGGIIYEQTPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
GNLVPELAAKSMPCGTQVITTEPLGEELARTLLPQDYCVEDCNYLLDYYRLTSDKRLIFG
GGVVYGARDPANIEAIIRPKMLKAFPQLKNVKIDYAWTGNFLLTLSRLPQVGRIGDNIYY
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFAGLPHYPFPGGQMLRVPFSAIGAWYYS
LRDRLGF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory