SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_2774 PP_2774 glycine betaine ABC transporter, ATPase/permease fusion protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
47% identity, 33% coverage: 47:278/698 of query aligns to 34:265/382 of 7ahhC

query
sites
7ahhC

S

K

R

K

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T

D

G

S

A

T
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T

N
x
V

A
x
G

V

V

E

Y

Q

D

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T

N

N

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F

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E

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I

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N

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G

E

E

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F

C

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I

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M

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G

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G

K
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K

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T

L

L

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R

H

L

V

L

N

N

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L

L

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I

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E

P

P

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G

G

E

K

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F

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L

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K

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I

M

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S

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M

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V

F

F

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Q

H

N

F

F

G

G

L

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F

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D

binding phosphoaminophosphonic acid-adenylate ester: T39 (= T52), V40 (≠ N53), G41 (≠ A54), S61 (= S74), G62 (= G75), S63 (= S76), G64 (= G77), K65 (= K78), S66 (= S79), T67 (= T80), D187 (= D200), E188 (= E201)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
47% identity, 33% coverage: 47:278/698 of query aligns to 34:265/382 of 7aheC

query
sites
7aheC

S

K

R

K

S

T

D

G

S

A

T

T

N

V

A

G

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E

Y

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G

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M

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G

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N

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I

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E

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N

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E

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Y

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L

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E

L

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G

N

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N

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A

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N

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Y

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D

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

7ahdC Opua (e190q) occluded (see paper)
47% identity, 33% coverage: 47:273/698 of query aligns to 34:260/260 of 7ahdC

query
sites
7ahdC

S

K

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K

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C

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N

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F

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G

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x
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K

binding adenosine-5'-triphosphate: T39 (= T52), S61 (= S74), G62 (= G75), S63 (= S76), G64 (= G77), K65 (= K78), S66 (= S79), T67 (= T80), Q111 (= Q124), K161 (≠ H174), Q162 (≠ E175), S164 (= S177), G166 (= G179), M167 (= M180), Q188 (≠ E201), H221 (= H234)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 41% coverage: 44:326/698 of query aligns to 12:289/343 of P30750

query
sites
P30750

Y

H

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Q

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G

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-

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x
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40:46 (vs. 74:80, 86% identical) binding
E166 (= E201) mutation to Q: Exhibits little ATPase activity.
VDAPLL 278:283 (≠ VLGPAA 315:320) binding

Sites not aligning to the query:

295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
38% identity, 41% coverage: 44:326/698 of query aligns to 13:290/344 of 6cvlD

query
sites
6cvlD

Y

H

E
x
Q

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G

S

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R

R

S

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D

-

S

-

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x
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E

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N

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L

L

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x
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x
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R

T

P

L

H

H

I

L

G

-

P

-

S

D

I

I

A

P

S

E

S

D

S

Y

D

Q

E

E

V

R

L

L

-

Q

S

A

E

E

P

P

A

F

S

T

A

D

E

C

L

V

P

P

A

M

R

L

S

R

T

-

I

L

H

E

W

F

I

T

R

G

Q

Q

S

S

V

V

L

D

G

A

P

P

A

L

L

S

A

E

A

T

A

A

G

R

R

R

binding phosphothiophosphoric acid-adenylate ester: Q14 (≠ E45), I19 (≠ T52), S41 (= S74), G42 (= G75), A43 (≠ S76), G44 (= G77), K45 (= K78), S46 (= S79), T47 (= T80), N141 (≠ E175), L142 (= L176), S143 (= S177), G144 (= G178), G145 (= G179), Q146 (≠ M180), H200 (= H234)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 12

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
38% identity, 41% coverage: 44:326/698 of query aligns to 13:290/344 of 3tuzC

query
sites
3tuzC

Y

H

E
x
Q

G

G

S

T

R

R

S

T

D

-

S

-

T

I

N

Q

A
|
A

V

L

E

N

Q

N

V

V

N

S

L

L

A

H

V

V

R

P

R

A

G

G

E

Q

V

I

L

Y

C

G

L

V

M

I

G

G

T

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

H

C

V

V

N

N

R

L

L

L

I

E

E

R

P

P

S

T

S

E

G

G

E

S

V

V

L

L

I

V

D

D

G

G

E

Q

V

E

L

L

S

T

L

L

T

S

Q

E

P

S

T

E

L

L

R

T

Q

K

L

A

R

R

S

R

Q

Q

R

-

I

I

G

G

M

M

V

I

F

F

Q

Q

H

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

D

G

N

N

V

V

A

A

L

L

P

P

L

L

E

E

L

L

R

D

G

N

E

T

P

P

E

K

S

D

T

E

R

V

R

K

T

R

A

R

A

V

L

T

R

E

Q

L

L

L

Q

S

A

L

V

V

G

G

L

L

K

G

A

D

W

K

S

H

E

D

R

S

F

Y

P

P

H

S

E

N

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

S

N

N

P

P

D

K

I

V

L

L

M

C

D

D

E

Q

P

A

F

T

S

S

A

A

L

L

D

D

P

P

T

A

I

T

R

T

R

R

D

S

L

I

Q

L

G

E

R

L

F

L

L

K

Q

D

L

I

V

N

R

R

E

R

R

L

G

G

I

L

T

T

T

I

L

L

V

I

T

T

H

H

D

E

P

M

G

D

E

V

A

V

L

K

R

R

L

I

A

C

D

D

R

C

I

V

A

A

V

V

L

I

R

S

G

N

G

G

R

E

L

L

I

I

Q

E

V

Q

G

D

T

T

P

V

G

S

E

E

L

V

L

F

E

S

H

H

P

P

A

K

D

T

E

P

G

L

V

A

A

Q

V

K

F

F

F

I

D

Q

E

S

R

T

P

L

H

H

I

L

G

-

P

-

S

D

I

I

A

P

S

E

S

D

S

Y

D

Q

E

E

V

R

L

L

-

Q

S

A

E

E

P

P

A

F

S

T

A

D

E

C

L

V

P

P

A

M

R

L

S

R

T

-

I

L

H

E

W
x
F

I

T

R

G

Q

Q

S

S

V
|
V

L

D

G
x
A

P
|
P

A
x
L

L

S

A

E

A

T

A

A

G

R

R

R

binding adenosine-5'-diphosphate: Q14 (≠ E45), A21 (= A54), G42 (= G75), A43 (≠ S76), G44 (= G77), K45 (= K78), S46 (= S79), T47 (= T80)
binding selenomethionine: F274 (≠ W310), V279 (= V315), A281 (≠ G317), P282 (= P318), L283 (≠ A319), L284 (≠ A320)

Sites not aligning to the query:

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
38% identity, 41% coverage: 44:326/698 of query aligns to 13:290/344 of 3tuiC

query
sites
3tuiC

Y

H

E
x
Q

G

G

S

T

R

R

S

T

D

-

S

-

T

I

N

Q

A

A

V

L

E

N

Q

N

V

V

N

S

L

L

A

H

V

V

R

P

R

A

G

G

E

Q

V

I

L

Y

C

G

L

V

M

I

G

G

T

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

H

C

V

V

N

N

R

L

L

L

I

E

E

R

P

P

S

T

S

E

G

G

E

S

V

V

L

L

I

V

D

D

G

G

E

Q

V

E

L

L

S

T

L

L

T

S

Q

E

P

S

T

E

L

L

R

T

Q

K

L

A

R

R

S

R

Q

Q

R

-

I

I

G

G

M

M

V

I

F

F

Q

Q

H

H

F

F

G

N

L

L

P

S

H

S

R

R

S

T

V

V

L

F

D

G

N

N

V

V

A

A

L

L

P

P

L

L

E

E

L

L

R

D

G

N

E

T

P

P

E

K

S

D

T

E

R

V

R

K

T

R

A

R

A

V

L

T

R

E

Q

L

L

L

Q

S

A

L

V

V

G

G

L

L

K

G

A

D

W

K

S

H

E

D

R

S

F

Y

P

P

H

S

E

N

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

S

N

N

P

P

D

K

I

V

L

L

M

C

D

D

E

Q

P

A

F

T

S

S

A

A

L

L

D

D

P

P

T

A

I

T

R

T

R

R

D

S

L

I

Q

L

G

E

R

L

F

L

L

K

Q

D

L

I

V

N

R

R

E

R

R

L

G

G

I

L

T

T

T

I

L

L

V

I

T

T

H

H

D

E

P

M

G

D

E

V

A

V

L

K

R

R

L

I

A

C

D

D

R

C

I

V

A

A

V

V

L

I

R

S

G

N

G

G

R

E

L

L

I

I

Q

E

V

Q

G

D

T

T

P

V

G

S

E

E

L

V

L

F

E

S

H

H

P

P

A

K

D

T

E

P

G

L

V

A

A

Q

V

K

F

F

F

I

D

Q

E

S

R

T

P

L

H

H

I

L

G

-

P

-

S

D

I

I

A

P

S

E

S

D

S

Y

D

Q

E

E

V

R

L

L

-

Q

S

A

E

E

P

P

A

F

S

T

A

D

E

C

L

V

P

P

A

M

R

L

S

R

T

-

I

L

H

E

W

F

I

T

R

G

Q

Q

S

S

V

V

L

D

G

A

P

P

A

L

L

S

A

E

A

T

A

A

G

R

R

R

binding adenosine-5'-diphosphate: Q14 (≠ E45), S41 (= S74), G42 (= G75), A43 (≠ S76), G44 (= G77), K45 (= K78), S46 (= S79), T47 (= T80)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
39% identity, 33% coverage: 47:275/698 of query aligns to 14:242/375 of 2d62A

query
sites
2d62A

S

K

R

R

S

F

D

G

S

D

T

V

N

T

A

A

V

V

E

K

Q

D

V

L

N

S

L

L

A

E

V

I

R

K

R

D

G

G

E

E

V

F

L

L

C

V

L

L

M

L

G

G

T
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

H

M

V

I

N

A

R

G

L

L

I

E

E

E

P

P

S

T

S

R

G

G

E

Q

V

I

L

Y

I

I

D

E

G

D

E

N

V

L

L

V

S

A

S

D

L

P

T

E

Q

K

P

G

T

V

L

F

R

V

Q

P

L

P

R

K

S

E

Q

R

R

D

I

V

G

A

M

M

V

V

F

F

Q

Q

H

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

Y

D

D

N

N

V

I

A

A

L

F

P

P

L

L

E

K

L

L

R

R

G

K

E

V

P

P

E

K

S

Q

T

E

R

I

R

D

T

K

A

R

A

V

L

R

R

E

Q

V

L

A

Q

E

A

M

V

L

G

G

L

L

K

T

A

E

W

L

S

L

E

N

R

R

F

K

P

P

H

R

E

E

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

L

A

G

R

R

A

A

L

I

V

I

T

R

N

R

P

P

D

K

I

V

L

F

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

T

K

I

L

R

R

R

V

D

K

L

M

Q

R

G

A

R

E

F

L

L

K

Q

K

L

L

V

Q

R

R

E

Q

R

L

G

G

I

V

T

T

L

I

L

Y

V

V

T

T

H

H

D

D

P

Q

G

V

E

E

A

A

L

M

R

T

L

M

A

G

D

D

R

R

I

I

A

A

V

V

L

M

R

N

G

K

G

G

R

E

L

L

I

Q

Q

Q

V

V

G

G

T

T

P

P

G

D

E

E

L

V

L

Y

E

Y

H

K

P

P

A

V

D

N

E

T

G

F

V

V

A

A

V

G

F

F

binding pyrophosphate 2-: P40 (≠ T73), S41 (= S74), G42 (= G75), C43 (≠ S76), G44 (= G77), K45 (= K78), T46 (≠ S79), T47 (= T80)

1g291 Malk (see paper)
42% identity, 32% coverage: 51:275/698 of query aligns to 15:239/372 of 1g291

query
sites
1g291

S

E

T

V

N

T

A

A

V

V

E

R

Q

E

V

M

N

S

L

L

A

E

V

V

R

K

R

D

G

G

E

E

V

F

L

M

C

I

L

L

M

L

G

G

T
x
P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

S
x
T

T
|
T

L

T

L

L

R

R

H

M

V

I

N

A

R

G

L

L

I

E

E

E

P

P

S

S

S

R

G

G

E

Q

V

I

L

Y

I

I

D

G

G

D

E

K

V

L

L

V

S

A

S
x
D

L

P

T
x
E

Q
x
K

P

G

T

I

L

F

R

V

Q

P

L

P

R
x
K

S
x
D

Q

R

R

D

I

I

G

A

M

M

V

V

F

F

Q

Q

H

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

Y

D

D

N

N

V

I

A

A

L

F

P

P

L

L

E

K

L

L

R

R

G

K

E

V

P

P

-

R

-

Q

E

E

S

I

T

D

R

Q

R

R

T

V

A

R

A

E

L

V

R

A

Q

E

L

L

Q

-

A

-

V

L

G

G

L

L

K

T

A

E

W

L

S

L

E

N

R

R

F

K

P

P

H

R

E

E

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

L

A

G

R

R

A

A

L

I

V

V

T

R

N

K

P

P

D

Q

I

V

L

F

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

T

K

I

L

R

R

R

V

D

R

L

M

Q

R

G

A

R

E

F

L

L

K

Q

K

L

L

V

Q

R

R

E

Q

R

L

G

G

I

V

T

T

L

I

L

Y

V

V

T

T

H

H

D

D

P

Q

G

V

E

E

A

A

L

M

R

T

L

M

A

G

D

D

R

R

I

I

A

A

V

V

L

M

R

N

G

R

G

G

R

V

L

L

I

Q

Q

Q

V

V

G

G

T

S

P

P

G

D

E

E

L

V

L
x
Y

E
x
D

H

K

P

P

A

A

D

N

E

T

G

F

V

V

A

A

V

G

F

F

binding magnesium ion: D69 (≠ S105), E71 (≠ T107), K72 (≠ Q108), K79 (≠ R115), D80 (≠ S116), Y228 (≠ L264), D229 (≠ E265)
binding pyrophosphate 2-: P37 (≠ T73), S38 (= S74), G39 (= G75), C40 (≠ S76), G41 (= G77), K42 (= K78), T43 (≠ S79), T44 (= T80)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 341, 342, 359

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 35% coverage: 50:292/698 of query aligns to 28:264/378 of P69874

query
sites
P69874

D

D

S

G

T

K

N

E

A

V

V

I

E

P

Q

Q

V

L

N

D

L

L

A

T

V

I

R

N

G

G

E

E

V
x
F

L

L

C

T

L

L

M

L

G

G

T

P

S

S

G

G

S
x
C

G

G

K

K

S

T

T

T

L

V

L
|
L

R

R

H

L

V

I

N

A

R

G

L

L

I

E

E

T

P

V

S

D

S

S

G

G

E

R

V

I

L

M

I
x
L

D

D

G

N

E

E

V

D

L

I

S

T

S

H

L

V

T

-

Q

-

P

P

T

A

L

-

R

-

Q

-

L

-

R

E

S

N

Q

R

R

Y

I

V

G

N

M

T

V

V

F

F

Q

Q

H

S

F

Y

G

A

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

F

D

E

N

N

V

V

A

A

L

F

P

G

L

L

E

R

L

M

R

Q

G

K

E

T

P

P

E

A

S

A

T

E

R

I

R

T

T

P

A

R

A

V

L

M

R

E

Q

A

L

L

Q

R

A

M

V
|
V

G

Q

L

L

K

E

A

T

W

F

S

A

E

Q

R

R

F

K

P

P

H

H

E

Q

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

V

V

V

T

N

N

K

P

P

D

R

I

L

L

L

M

L

D
|
D

E

E

P

S

F

L

S

S

A

A

L

L

D

D

P

Y

T

K

I

L

R

R

R

K

D

Q

L

M

Q

Q

G

N

R

E

F

L

L

K

Q

A

L

L

V

Q

R

R

E

K

R

L

G

G

I

I

T

T

T

F

L

V

L

F

V

V

T

T

H

H

D

D

P

Q

G

E

E

E

A

A

L

L

R

T

L

M

A

S

D

D

R

R

I

I

A

V

V

V

L

M

R

R

G

D

G

G

R

R

L

I

I

E

Q

Q

V

D

G

G

T

T

P

P

G

R

E

E

L

I

L

Y

E

E

H

E

P

P

A

K

D

N

E

L

G

F

V

V

A

A

V

G

F

F

F

I

D

G

E

E

R

I

P

N

H

M

I

F

G

N

P

A

S

T

I

V

A

I

S

E

S

R

S

L

D

D

E

E

F45 (≠ V67) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S76) mutation to T: Loss of ATPase activity and transport.
L60 (= L82) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I98) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V163) mutation to M: Loss of ATPase activity and transport.
D172 (= D200) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
43% identity, 31% coverage: 58:276/698 of query aligns to 21:236/241 of 4u00A

query
sites
4u00A

V

I

N

H

L

L

A

E

V

V

R

A

R

P

G

G

E

E

V

K

L

L

C

V

L

I

M

I

G

G

T

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

H

T

V

I

N

N

R

R

L

L

I

E

E

D

P

F

S

Q

S

E

G

G

E

E

V

V

L

V

I

V

D

D

G

G

E

-

V

-

L

L

S

S

S

V

L

K

T

D

Q

D

P

R

T

A

L

L

R

R

Q

E

L

I

R

R

S

R

Q

E

R

-

I

V

G

G

M

M

V

V

F

F

Q

Q

H

Q

F

F

G

N

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

L

D

E

N

N

V

V

A

T

L

L

-

A

P

P

L

M

E

R

L

V

R

R

G

R

E

W

P

P

E

R

S

E

T

K

R

A

R

E

T

K

A

K

A

A

L

L

R

E

Q

L

L

L

Q

E

A

R

V

V

G

G

L

I

K

L

A

D

W

Q

S

A

E

R

R

K

F

Y

P

P

H

A

E

Q

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

E

P

P

D

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

-

I

-

R

-

D

E

L

M

Q

V

G

G

R

E

F

V

L

L

Q

D

L

V

V

M

R

R

E

D

R

L

-

A

-

Q

-

G

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H

H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

A

D

D

R

R

I

V

A

V

V

F

L

M

R

D

G

G

R

Q

L

I

I

V

Q

E

V

E

G

G

T

R

P

P

G

E

E

E

L

I

L

F

E

T

H

R

P

P

A

K

D

E

E

E

G

R

V

T

A

R

V

S

F

F

F

L

binding adenosine-5'-diphosphate: S37 (= S74), G38 (= G75), S39 (= S76), G40 (= G77), K41 (= K78), S42 (= S79), T43 (= T80)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12, 15, 17

3c4jA Abc protein artp in complex with atp-gamma-s
41% identity, 32% coverage: 51:275/698 of query aligns to 15:237/242 of 3c4jA

query
sites
3c4jA

S

S

T

L

N

E

A
x
V

V

L

E

K

Q

G

V

I

N

N

L

V

A

H

V

I

R

R

R

E

G

G

E

E

V

V

L

V

C

V

L

V

M

I

G

G

T
x
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

H

C

V

L

N

N

R

L

L

L

I

E

E

D

P

F

S

D

S

E

G

G

E

E

V

I

L

I

I

I

D

D

G

G

E

I

V

N

L

L

S

K

S

A

L

-

T

K

Q

D

P

T

T

N

L

L

R

N

Q

K

L

V

R

R

S

E

Q

E

R

-

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

G

N

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

L

D

N

N

N

V

I

A

T

L

L

-

A

P

P

L

M

E

K

L

V

R

R

G

K

E

W

P

P

E

R

S

E

T

K

R

A

R

E

T

A

A

K

A

A

L

M

R

E

Q

L

L

L

Q

D

A

K

V

V

G

G

L

L

K

K

A

D

W

K

S

A

E
x
H

R

A

F
x
Y

P

P

H

D

E

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

E

P

P

D

K

I

I

L

M

L

L

M

F

D

D

E
|
E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

E

I

M

R

V

R

G

D

E

L

V

Q

L

G

S

R

V

F

M

L

K

Q

Q

L

L

V

A

R

N

E

E

R

-

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H

H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

G

D

D

R

R

I

V

A

L

V

F

L

M

R

D

G

G

R

Y

L

I

I

I

Q

E

V

E

G

G

T

K

P

P

G

E

E

D

L

L

L

F

E

D

H

R

P

P

A

Q

D

H

E

E

G

R

V

T

A

K

V

A

F

F

binding phosphothiophosphoric acid-adenylate ester: V18 (≠ A54), P37 (≠ T73), S38 (= S74), G39 (= G75), S40 (= S76), G41 (= G77), K42 (= K78), S43 (= S79), T44 (= T80), H133 (≠ E170), Y135 (≠ F172), E164 (= E201)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 13

3c41J Abc protein artp in complex with amp-pnp/mg2+
41% identity, 32% coverage: 51:275/698 of query aligns to 15:237/242 of 3c41J

query
sites
3c41J

S

S

T

L

N

E

A
x
V

V

L

E

K

Q

G

V

I

N

N

L

V

A

H

V

I

R

R

R

E

G

G

E

E

V

V

L

V

C

V

L

V

M

I

G

G

T
x
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R
|
R

H

C

V

L

N

N

R

L

L

L

I

E

E

D

P

F

S

D

S

E

G

G

E

E

V

I

L

I

I

I

D

D

G

G

E

I

V

N

L

L

S

K

S

A

L

-

T

K

Q

D

P

T

T

N

L

L

R

N

Q

K

L

V

R

R

S

E

Q

E

R

-

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

G

N

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

L

D

N

N

N

V

I

A

T

L

L

-

A

P

P

L

M

E

K

L

V

R

R

G

K

E

W

P

P

E

R

S

E

T

K

R

A

R

E

T

A

A

K

A

A

L

M

R

E

Q

L

L

L

Q

D

A

K

V

V

G

G

L

L

K

K

A

D

W

K

S

A

E
x
H

R

A

F
x
Y

P

P

H

D

E

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

E

P

P

D

K

I

I

L

M

L

L

M

F

D
|
D

E

E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

E

I

M

R

V

R

G

D

E

L

V

Q

L

G

S

R

V

F

M

L

K

Q

Q

L

L

V

A

R

N

E

E

R

-

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H

H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

G

D

D

R

R

I

V

A

L

V

F

L

M

R

D

G

G

R

Y

L

I

I

I

Q

E

V

E

G

G

T

K

P

P

G

E

E

D

L

L

L

F

E

D

H

R

P

P

A

Q

D

H

E

E

G

R

V

T

A

K

V

A

F

F

binding phosphoaminophosphonic acid-adenylate ester: V18 (≠ A54), P37 (≠ T73), S38 (= S74), G39 (= G75), S40 (= S76), G41 (= G77), K42 (= K78), S43 (= S79), T44 (= T80), R47 (= R83), H133 (≠ E170), Y135 (≠ F172)
binding magnesium ion: S43 (= S79), D163 (= D200)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

2olkA Abc protein artp in complex with adp-beta-s
41% identity, 32% coverage: 51:275/698 of query aligns to 15:237/242 of 2olkA

query
sites
2olkA

S

S

T
x
L

N

E

A
x
V

V

L

E

K

Q

G

V

I

N

N

L

V

A

H

V

I

R

R

R

E

G

G

E

E

V

V

L

V

C

V

L

V

M

I

G

G

T

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

H

C

V

L

N

N

R

L

L

L

I

E

E

D

P

F

S

D

S

E

G

G

E

E

V

I

L

I

I

I

D

D

G

G

E

I

V

N

L

L

S

K

S

A

L

-

T

K

Q

D

P

T

T

N

L

L

R

N

Q

K

L

V

R

R

S

E

Q

E

R

-

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

G

N

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

L

D

N

N

N

V

I

A

T

L

L

-

A

P

P

L

M

E

K

L

V

R

R

G

K

E

W

P

P

E

R

S

E

T

K

R

A

R

E

T

A

A

K

A

A

L

M

R

E

Q

L

L

L

Q

D

A

K

V

V

G

G

L

L

K

K

A

D

W

K

S

A

E

H

R

A

F

Y

P

P

H

D

E

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

E

P

P

D

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

E

I

M

R

V

R

G

D

E

L

V

Q

L

G

S

R

V

F

M

L

K

Q

Q

L

L

V

A

R

N

E

E

R

-

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H

H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

G

D

D

R

R

I

V

A

L

V

F

L

M

R

D

G

G

R

Y

L

I

I

I

Q

E

V

E

G

G

T

K

P

P

G

E

E

D

L

L

L

F

E

D

H

R

P

P

A

Q

D

H

E

E

G

R

V

T

A

K

V

A

F

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: L16 (≠ T52), V18 (≠ A54), S38 (= S74), G39 (= G75), S40 (= S76), G41 (= G77), K42 (= K78), S43 (= S79), T44 (= T80)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 13

2oljA Abc protein artp in complex with adp/mg2+
41% identity, 32% coverage: 51:275/698 of query aligns to 15:237/242 of 2oljA

query
sites
2oljA

S

S

T

L

N

E

A
x
V

V

L

E

K

Q

G

V

I

N

N

L

V

A

H

V

I

R

R

R

E

G

G

E

E

V

V

L

V

C

V

L

V

M

I

G

G

T

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

R

R

H

C

V

L

N

N

R

L

L

L

I

E

E

D

P

F

S

D

S

E

G

G

E

E

V

I

L

I

I

I

D

D

G

G

E

I

V

N

L

L

S

K

S

A

L

-

T

K

Q

D

P

T

T

N

L

L

R

N

Q

K

L

V

R

R

S

E

Q

E

R

-

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

G

N

L

L

L

F

P

P

H

H

R

M

S

T

V

V

L

L

D

N

N

N

V

I

A

T

L

L

-

A

P

P

L

M

E

K

L

V

R

R

G

K

E

W

P

P

E

R

S

E

T

K

R

A

R

E

T

A

A

K

A

A

L

M

R

E

Q

L

L

L

Q

D

A

K

V

V

G

G

L

L

K

K

A

D

W

K

S

A

E
x
H

R

A

F
x
Y

P

P

H

D

E

S

L

L

S

S

G

G

M

Q

Q

A

Q

Q

R

R

V

V

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

E

P

P

D

K

I

I

L

M

L

L

M

F

D

D

E

E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

E

I

M

R

V

R

G

D

E

L

V

Q

L

G

S

R

V

F

M

L

K

Q

Q

L

L

V

A

R

N

E

E

R

-

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H

H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

G

D

D

R

R

I

V

A

L

V

F

L

M

R

D

G

G

R

Y

L

I

I

I

Q

E

V

E

G

G

T

K

P

P

G

E

E

D

L

L

L

F

E

D

H

R

P

P

A

Q

D

H

E

E

G

R

V

T

A

K

V

A

F

F

binding adenosine-5'-diphosphate: V18 (≠ A54), S38 (= S74), G39 (= G75), S40 (= S76), G41 (= G77), K42 (= K78), S43 (= S79), T44 (= T80), H133 (≠ E170), Y135 (≠ F172)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
40% identity, 31% coverage: 58:270/698 of query aligns to 20:230/240 of 4ymuJ

query
sites
4ymuJ

V

V

N

T

L

L

A

K

V

V

R

N

R

K

G

G

E

E

V

V

L

V

C

V

L

I

M

I

G

G

T

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

H

C

V

I

N

N

R

L

L

L

I
x
E

E

E

P

P

S

T

S

K

G

G

E

E

V

V

L

F

I

I

D

D

G

G

E

V

V

K

L

I

S

N

L

-

T

G

Q

K

P

V

T

N

L

I

R

N

Q

K

L

V

R

R

S

-

Q

Q

R

K

I

V

G

G

M

M

V

V

F

F

Q

Q

H

H

F

F

G

N

L

L

L

F

P

P

H

H

R

L

S

T

V

A

L

I

D

E

N

N

V

I

A

T

L

L

-

A

P

P

L

V

E

K

L

V

R

K

G

K

E

M

P

N

E

K

S

K

T

E

R

A

R

E

T

E

A

L

A

A

L

V

R

D

Q

L

L

L

Q

A

A

K

V

V

G

G

L

L

K

L

A

D

W

K

S

K

E

D

R

Q

F

Y

P

P

H

I

E

K

L

L

S

S

G

G

M

Q

Q

K

Q

Q

R

R

V

L

G

A

L

I

A

A

R

R

A

A

L

L

V

A

T

M

N

Q

P

P

D

E

I

V

L

M

L

L

M

F

D

D

E
|
E

P

P

F

T

S

S

A

A

L

L

D

D

P

P

T

E

I

M

R

V

R

K

D

E

L

V

Q

L

G

N

R

V

F

M

L

K

Q

Q

L

L

V

A

R

N

E

E

R

-

G

G

I

M

T

T

T

M

L

V

V

V

T

T

H
|
H

D

E

P

M

G

G

E

F

A

A

L

R

R

E

L

V

A

G

D

D

R

R

I

V

A

I

V

F

L

M

R

D

G

D

G

G

R

V

L

I

I

V

Q

E

V

E

G

G

T

T

P

P

G

E

E

E

L

I

L

F

E

Y

H

R

P

A

A

K

D

N

E

E

binding adenosine-5'-triphosphate: S36 (= S74), G37 (= G75), S38 (= S76), G39 (= G77), K40 (= K78), S41 (= S79), T42 (= T80), E51 (≠ I89), E162 (= E201), H194 (= H234)
binding magnesium ion: S41 (= S79), E162 (= E201)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11, 14, 16

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 33% coverage: 47:275/698 of query aligns to 12:234/393 of P9WQI3

query
sites
P9WQI3

S

S

R

Y

S

P

D

D

S

G

T

H

N

T

A

A

V

V

E

R

Q

D

V

L

N

N

L

L

A

T

V

I

R

A

R

D

G

G

E

E

V

F

L

L

C

I

L

L

M

V

G

G

T

P

S

S

G

G

S

C

G

G

K

K

S

T

T

T

L

T

L

L

R

N

H

M

V

I

N

A

R

G

L

L

I

E

E

D

P

I

S

S

G

G

E

E

V

L

L

R

I

I

D

A

G

G

E

E

V

R

L

V

S

N

S

E

L

-

T

-

Q

-

P

-

T

-

L

-

R

K

Q

A

L

P

R

K

S

D

Q

R

R

D

I

I

G

A

M

M

V

V

F

F

Q

Q

H

S

F

Y

G

A

L

L

L

Y

P

P

H

H

R

M

S

T

V

V

L

R

D

Q

N

N

V

I

A

A

L

F

P

P

L

L

E

T

L

L

R

A

G

K

E

M

P

R

E

K

S

A

T

D

R

I

R

A

T

Q

A

K

A

V

L

S

R

E

Q

T

L

A

Q

K

A

I

V

L

G

D

L

L

K

T

A

N

W

L

S

L

E

D

R

R

F

K

P

P

H

S

E

Q

L

L

S

S

G

G

M

Q

Q

R

Q

Q

R

R

V

V

G

A

L

M

A

G

R

R

A

A

L

I

V

V

T

R

N

H

P

P

D

K

I

A

L

F

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

T

K

I

L

R

R

R

V

D

Q

L

M

Q

R

G

G

R

E

F

I

L

A

Q

Q

L

L

V

Q

R

R

E

R

R

L

G

G

I

T

T

T

L

V

L

Y

V

V

T

T

H
|
H

D

D

P

Q

G

T

E

E

A

A

L

M

R

T

L

L

A

G

D

D

R

R

I

V

A

V

V

V

L

M

R

Y

G

G

R

I

L

A

I

Q

Q

Q

V

I

G

G

T

T

P

P

G

E

E

E

L

L

L

Y

E

E

H

R

P

P

A

A

D

N

E

L

G

F

V

V

A

A

V

G

F

F

H193 (= H234) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
38% identity, 32% coverage: 54:278/698 of query aligns to 20:243/353 of 1oxvD

query
sites
1oxvD

A
|
A

V

L

E

D

Q

N

V

V

N

N

L

I

A

N

V

I

R

E

R

N

G

G

E

E

V

R

L

F

C

G

L

I

M

L

G

G

T
x
P

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

L

M

R

R

H

I

V

I

N

A

R

G

L

L

I

D

E

V

P

P

S

S

S

T

G

G

E

E

V

L

L

Y

I

F

D

D

G

D

E

R

V

L

L

V

S

A

S

S

-

N

-

G

-

K

L

L

T

I

Q

V

P

P

T

P

L

-

R

-

Q

-

L

-

R

E

S

D

Q

R

R

K

I

I

G

G

M

M

V

V

F

F

Q
|
Q

H

T

F

W

G

A

L

L

L

Y

P

P

H

N

R

L

S

T

V

A

L

F

D

E

N

N

V

I

A

A

L

F

P

P

L

L

-

T

E

N

L

M

R

K

G

M

E

S

P

K

E

E

S

E

T

I

R

R

R

K

T

R

A

V

A

E

L

-

R

E

Q

V

L

A

Q

K

A

I

V

L

G

D

L

I

K

H

A

H

W

V

S

L

E

N

R

H

F

F

P

P

H

R

E

E

L

L

S

S

G

G

M

Q

Q

Q

R

R

V

V

G

A

L

L

A

A

R

R

A

A

L

L

V

V

T

K

N

D

P

P

D

S

I

L

L

L

M

L

D

D

E
|
E

P

P

F

F

S

S

A

N

L

L

D

D

P

A

T

R

I

M

R

R

R

D

D

S

L

A

Q

R

G

A

R

L

F

V

L

K

Q

E

L

V

V

Q

R

S

E

R

R

L

G

G

I

V

T

T

T

L

L

L

L

V

V

V

T

S

H

H

D

D

P

P

G

A

E

D

A

I

L

F

R

A

L

I

A

A

D

D

R

R

I

V

A

G

V

V

L

L

R

V

G

K

G

G

R

K

L

L

I

V

Q

Q

V

V

G

G

T

K

P

P

G

E

E

D

L

L

L

Y

E

D

H

N

P

P

A

V

D

S

E

I

G

Q

V

V

A

A

V

S

F

L

F

I

D

G

E

E

binding phosphoaminophosphonic acid-adenylate ester: A20 (= A54), P39 (≠ T73), S40 (= S74), G41 (= G75), A42 (≠ S76), G43 (= G77), K44 (= K78), T45 (≠ S79), T46 (= T80), Q89 (= Q124), E166 (= E201)
binding magnesium ion: T45 (≠ S79), Q89 (= Q124)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13, 18

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
38% identity, 32% coverage: 54:278/698 of query aligns to 20:243/353 of 1oxvA

query
sites
1oxvA

A
|
A

V

L

E

D

Q

N

V

V

N

N

L

I

A

N

V

I

R

E

R

N

G

G

E

E

V

R

L

F

C

G

L

I

M

L

G

G</