SitesBLAST
Comparing PP_2837 FitnessBrowser__Putida:PP_2837 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 16 hits to proteins with known functional sites (download)
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
30% identity, 94% coverage: 6:415/436 of query aligns to 12:423/430 of P0AA76
- Y29 (= Y23) binding
- D31 (= D25) mutation to N: Loss of galactonate transport activity.
- R32 (= R26) binding
- Y64 (= Y58) binding
- E118 (= E112) mutation to Q: Loss of galactonate transport activity.
- W358 (= W349) binding
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
30% identity, 94% coverage: 6:415/436 of query aligns to 1:404/409 of 6e9nA
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
28% identity, 94% coverage: 6:415/436 of query aligns to 4:388/393 of 6e9oA
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
21% identity, 90% coverage: 27:419/436 of query aligns to 88:482/495 of Q9NRA2
- K136 (= K75) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ A120) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ PT 135:136) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ -L 202) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSLRN 268:272 (≠ PSERQ 203:207) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (≠ A268) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (≠ A274) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (= G310) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 92% coverage: 16:414/436 of query aligns to 91:497/582 of Q9JI12
- H128 (≠ L51) mutation to A: Greatly lowers L-glutamate transport.
- R184 (= R105) mutation R->A,E,K: Greatly lowers L-glutamate transport.
- E191 (= E112) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
- R322 (≠ V241) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.
Sites not aligning to the query:
- 88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
22% identity, 91% coverage: 18:414/436 of query aligns to 35:439/452 of 7t3nA
Sites not aligning to the query:
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 72% coverage: 29:342/436 of query aligns to 90:399/495 of Q5Q0U0
- K136 (≠ G79) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R105) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L108) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G109) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E112) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A113) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F116) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P117) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ A120) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ L123) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- SSLKN 268:272 (≠ ERQAA 205:209) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (≠ A274) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (= G310) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 72% coverage: 29:342/436 of query aligns to 90:399/495 of Q8BN82
- H183 (≠ A120) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
23% identity, 69% coverage: 32:330/436 of query aligns to 68:369/493 of Q03567
- N69 (= N33) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
22% identity, 86% coverage: 43:419/436 of query aligns to 106:485/497 of Q9Y2C5
- A372 (≠ I308) to T: in dbSNP:rs11754288
Sites not aligning to the query:
- 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
- 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
- 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
24% identity, 62% coverage: 2:273/436 of query aligns to 21:306/448 of Q51955
- D41 (≠ N22) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- D44 (= D25) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
- G85 (= G66) mutation to V: Abolishes 4-HBA transport and chemotaxis.
- D89 (= D70) mutation to N: Abolishes 4-HBA transport and chemotaxis.
- G92 (= G73) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
- R124 (= R105) mutation to A: Abolishes 4-HBA transport.
- E144 (= E125) mutation to A: Strong decrease in 4-HBA transport.
- H183 (≠ R164) mutation to A: Decrease in 4-HBA transport and chemotaxis.
Sites not aligning to the query:
- 323 D→N: Abolishes 4-HBA transport and chemotaxis.
- 328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
- 386 R→A: Strong decrease in 4-HBA transport.
- 398 R→A: Abolishes 4-HBA transport.
- 444 H→A: No change in 4-HBA transport and chemotaxis.
Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
26% identity, 43% coverage: 6:194/436 of query aligns to 111:299/605 of Q9GQQ0
- E217 (= E112) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.
Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 97% coverage: 3:426/436 of query aligns to 15:442/452 of Q5EXK5
- D82 (= D70) mutation to A: Loss of activity.
- V311 (≠ R287) mutation to W: Loss of activity.
- D314 (= D290) mutation to A: Loss of activity.
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 43% coverage: 9:194/436 of query aligns to 97:293/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
A2SWM2 Sphingosine-1-phosphate transporter SPNS2; Protein spinster homolog 2; Protein two of hearts from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
26% identity, 26% coverage: 19:132/436 of query aligns to 65:180/504 of A2SWM2
- R153 (= R105) mutation to S: In ko157; displays cardia bifida (2 hearts).
P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
26% identity, 67% coverage: 10:300/436 of query aligns to 15:285/403 of P77589
- E27 (≠ N22) mutation to A: Lack of 3HPP transport activity.; mutation to D: Slight decrease in 3HPP transport activity.
- D75 (= D70) mutation D->A,E: Lack of 3HPP transport activity.
- A272 (≠ R287) mutation to H: 30% increase in 3HPP transport activity.
- K276 (≠ W291) mutation to D: Lack of 3HPP transport activity.
Query Sequence
>PP_2837 FitnessBrowser__Putida:PP_2837
MNPMKKYQRITVVFLLLIGIVNYLDRSALSIANTSIQKDMMISPSQMGILLSAFSIAYAF
AQLPMGMIIDRLGSKIALGASLLGWSVAQAAFGMVNSFAGFMGLRVLLGIGEAPMFPSAA
KALSEWFDANERGTPTGVVWSSTCLGPCLAPPLLTLFMVNFGWRGMFIITGVIGVVLALC
WLTFYKSKARFLAELAAEGKPLPSERQAAAATAPKASYFAGWLDLFKHRSTWGAVLGFMG
VIYMLWLHLTWLPGYFEREHGLDLYKTAWVVSLAYGFGAAGTIVAGRFCDWLVRRGMSVL
GSRKFSVITGLVLAALFTLPLSFVTGLTGCIMLLCLALFSINMASATAWMIVNTIVDSQR
VASFGSIQNFGGYIAGSVAPIVTGFSIQYSGSFTTAFMISAVVALCSAVAYFLLLQAPIG
SAKVEAGGMAGATEQA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory