SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_3274 FitnessBrowser__Putida:PP_3274 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P33164 Phthalate dioxygenase reductase; PDR; EC 1.-.-.- from Burkholderia cepacia (Pseudomonas cepacia) (see paper)
29% identity, 84% coverage: 54:355/358 of query aligns to 55:322/322 of P33164

query
sites
P33164

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RT 56:57 (≠ RS 55:56) binding
AVK 73:75 (= AVK 71:73) binding
RGGS 81:84 (≠ RFSA 79:82) binding
T125 (= T125) binding
F226 (= F244) binding
C273 (= C304) binding
S275 (≠ A306) binding
C278 (= C309) binding
C281 (= C312) binding
C309 (= C342) binding

2piaA Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2fe-2s] (see paper)
29% identity, 84% coverage: 54:355/358 of query aligns to 54:321/321 of 2piaA

query
sites
2piaA

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binding fe2/s2 (inorganic) cluster: S270 (≠ Y302), C272 (= C304), E273 (≠ K305), G275 (= G307), C277 (= C309), G278 (≠ S310), C280 (= C312), C308 (= C342)
binding flavin mononucleotide: R55 (= R55), T56 (≠ S56), Y57 (= Y57), S58 (= S58), A72 (= A71), V73 (= V72), G79 (= G78), R80 (= R79), G82 (≠ S81), S83 (≠ A82), I121 (≠ S122), T124 (= T125), E223 (= E242), F225 (= F244)

Sites not aligning to the query:

binding flavin mononucleotide: 44

1rfkB Crystal structure of 2fe2s ferredoxin from thermophilic cyanobacterium mastigocladus laminosus (see paper)
41% identity, 25% coverage: 265:352/358 of query aligns to 1:89/97 of 1rfkB

query
sites
1rfkB

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binding fe2/s2 (inorganic) cluster: Y39 (= Y302), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

6laaA Crystal structure of full-length cyp116b46 from tepidiphilus thermophilus (see paper)
27% identity, 84% coverage: 55:355/358 of query aligns to 487:753/753 of 6laaA

query
sites
6laaA

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binding fe2/s2 (inorganic) cluster: S700 (≠ Y302), C702 (= C304), E703 (≠ K305), G705 (= G307), C707 (= C309), G708 (≠ S310), C710 (= C312), C740 (= C342)
binding flavin mononucleotide: R487 (= R55), Q488 (≠ S56), Y489 (= Y57), S490 (= S58), Q506 (≠ K73), S511 (≠ G78), R512 (= R79), G514 (vs. gap), S515 (= S81), I553 (≠ S122), E651 (= E242), F653 (= F244)

Sites not aligning to the query:

active site: 177, 251, 252, 359, 360, 361
binding carbonate ion: 90, 91, 92, 241
binding protoporphyrin ix containing fe: 54, 91, 92, 99, 103, 249, 252, 253, 298, 351, 352, 353, 357, 359, 361

P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
27% identity, 68% coverage: 6:247/358 of query aligns to 102:333/334 of P0DPQ8

query
sites
P0DPQ8

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T

L

L

L

L

Y

Y

H

G

G

N

V

R

R

S

G

S

A

S

A

G

D

A

L

L

Y

F

D

R

L

D

G

K

R

L

F

E

A

D

E

L

I

K

A

N

E

R

E

Y

H

L

P

D

G

R

-

L

F

N

R

L

F

I

V

F

P

V

V

F

L

S

S

R

D

E

E

Q

P

Q

D

D

P

V

A

D

-

L

-

Y

Y

N

R

G

G

R

G

I

F

D

P

A

T

D

D

-

A

-

F

-

V

-

E

-

D

-

V

K

P

C

S

G

G

Q

R

L

G

F

W

S

S

R

G

W

W

L

L

D

-

V

-

A

-

G

-

L

-

D

-

A

-

A

-

F

-

I

-

C

C

G

G

P

P

Q

P

A

A

M

M

T

V

E

E

T

A

V

G

R

V

D

K

S

A

L

F

Q

K

A

R

N

R

G

R

M

M

A

S

K

P

E

R

R

R

I

I

H

H

F

R

E

E

L

K

F
|
F

A

T

A

P

A

A

RQYS 145:148 (≠ RSYS 55:58) binding
HVR 162:164 (≠ AVK 71:73) binding
VAT 170:172 (≠ RFS 79:81) binding
T215 (≠ S122) binding
F330 (= F244) binding

Sites not aligning to the query:

35 binding
40 binding
43 binding
75 binding
334 binding

5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
27% identity, 68% coverage: 6:247/358 of query aligns to 101:332/333 of 5ogxA

query
sites
5ogxA

S

T

L

A

T

T

I

V

K

L

Q

E

V

V

R

A

N

D

E

I

T

A

R

R

D

D

A

T

V

R

S

R

I

V

A

L

F

L

D

G

V

L

P

A

E

E

H

P

L

L

Q

A

G

-

Q

-

F

-

R

-

F

F

T

E

Q

A

G

G

Q

Q

Y
|
Y

L

V

V

E

M

L

R

-

T

-

Q

V

L

V

D

P

N

G

E

S

E

G

V

A

R

R

R
|
R

S
x
Q

Y
|
Y

S
|
S

I

L

C

A

-

N

S

T

A

A

V

D

Q

E

D

D

G

K

E

V

L

L

R

E

V

L

A
x
H

V
|
V

K

R

R

R

V
|
V

P

P

G

G

G
|
G

R
x
V

F
x
A

S
x
T

-

D

A

G

F

W

A

L

N

F

E

D

V

G

L

L

K

A

A

A

G

G

Q

D

Q

R

L

V

E

E

V

A

M

T

P

G

P

P

S

L

G

G

S

D

F

F

F

H

V

L

P

P

L

P

-

P

D

D

P

E

A

D

R

D

Q

G

G

G

N

P

Y

M

L

V

G

L

V

I

A

G

A

G

G

G

S
x
T

G

G

I

L

T

A

P

P

I

L

L

V

S

G

I

I

I

A

A

R

T

T

T

A

L

L

A

A

S

R

E

H

P

P

H

S

S

R

R

E

F

V

T

L

L

L

L

Y

Y

H

G

G

N

V

R

R

S

G

S

A

S

A

G

D

A

L

L

Y

F

D

R

L

D

G

K

R

L

F

E

A

D

E

L

I

K

A

N

E

R

E

Y

H

L

P

D

G

R

-

L

F

N

R

L

F

I

V

F

P

V

V

F

L

S

S

R

D

E

E

Q

P

Q

D

D

P

V

A

D

-

L

-

Y

Y

N

R

G

G

R

G

I

F

D

P

A

T

D

D

-

A

-

F

-

V

-

E

-

D

-

V

K

P

C

S

G

G

Q

R

L

G

F

W

S

S

R

G

W

W

L

L

D

-

V

-

A

-

G

-

L

-

D

-

A

-

A

-

F

-

I

-

C

C

G

G

P

P

Q

P

A

A

M

M

T

V

E

E

T

A

V

G

R

V

D

K

S

A

L

F

Q

K

A

R

N

R

G

R

M

M

A

S

K

P

E

R

R

R

I

I

H

H

F

R

E

E

L

K

F
|
F

A

T

A
x
P

A

A

binding flavin-adenine dinucleotide: Y132 (= Y41), R144 (= R55), Q145 (≠ S56), Y146 (= Y57), S147 (= S58), H161 (≠ A71), V162 (= V72), V165 (= V75), G168 (= G78), V169 (≠ R79), A170 (≠ F80), T171 (≠ S81), T214 (≠ S122), F329 (= F244), P331 (≠ A246)

Sites not aligning to the query:

3b2gA Leptolyngbya boryana ferredoxin (see paper)
39% identity, 24% coverage: 269:353/358 of query aligns to 5:90/98 of 3b2gA

query
sites
3b2gA

I

V

T

T

V

L

I

I

S

N

D

E

G

T

R

E

A

G

L

L

-

N

-

T

S

T

F

I

D

E

L

V

P

P

R

-

N

D

T

D

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

Q

G

G

A

I

E

D

L

L

P

P

Y

Y

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

I

I

T

E

A

G

G

E

T

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

Q

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

C

V

T

V

I

L

L

binding fe2/s2 (inorganic) cluster: S40 (= S303), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

6yacN Plant psi-ferredoxin supercomplex (see paper)
41% identity, 23% coverage: 271:352/358 of query aligns to 8:87/97 of 6yacN

query
sites
6yacN

V

V

I

T

S

P

D

D

G

G

R

T

A

Q

L

-

S

E

F

F

D

E

L

C

P

P

R

S

N

D

T

V

Q

Y

N

-

V

I

L

L

D

D

A

H

G

A

N

E

A

E

I

V

G

G

A

I

E

D

L

L

P

P

Y
|
Y

S
|
S

C
|
C

K
x
R

A

A

G

G

V

S

C
|
C

S

S

T
x
S

C
|
C

K

A

C

G

R

K

V

V

I

V

E

G

G

G

E

E

V

V

E

D

M

Q

D

S

S

D

N

G

H

S

A

F

L

L

E

D

D

D

Y

E

E

Q

V

I

A

E

A

A

G

G

Y

F

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

V

V

V

V

I

binding fe2/s2 (inorganic) cluster: Y37 (= Y302), S38 (= S303), C39 (= C304), R40 (≠ K305), C44 (= C309), S46 (≠ T311), C47 (= C312), C77 (= C342)

7fixR1 Photosystem I reaction center subunit PsaK (see paper)
38% identity, 25% coverage: 265:353/358 of query aligns to 1:89/97 of 7fixR1

query
sites
7fixR1

A

A

L

T

S

Y

H

K

I

V

T

T

V

L

I

V

-

R

S

P

D

D

G

G

R

S

A

E

L

T

S

T

F

I

D

D

L

V

P

P

R

E

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

V

G

A

N

E

A

E

I

Q

G

G

A

L

E

D

L

L

P

P

Y
x
F

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

L

I

L

E

E

G

G

E

E

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

K

G

G

Y

F

V

V

L
|
L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

R

S

S

D

D

K

C

V

K

V

I

L

L

binding [2Ga-2S] cluster: F38 (≠ Y302), S39 (= S303), C40 (= C304), R41 (≠ K305), G43 (= G307), C45 (= C309), C48 (= C312), L76 (= L340), C78 (= C342)

6khi1 Supercomplex for cylic electron transport in cyanobacteria (see paper)
38% identity, 25% coverage: 265:353/358 of query aligns to 2:90/98 of 6khi1

query
sites
6khi1

A

A

L

T

S

Y

H

K

I

V

T

T

V

L

I

V

-

R

S

P

D

D

G

G

R

S

A

E

L

T

S

T

F

I

D

D

L

V

P

P

R

E

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

V

G

A

N

E

A

E

I

Q

G

G

A

L

E

D

L

L

P

P

Y

F

S

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

L

I

L

E

E

G

G

E

E

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

K

G

G

Y

F

V

V

L
|
L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

R

S

S

D

D

K

C

V

K

V

I

L

L

binding fe2/s2 (inorganic) cluster: C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), L77 (= L340), C79 (= C342)

5h57A Ferredoxin iii from maize root (see paper)
43% identity, 22% coverage: 274:352/358 of query aligns to 11:88/97 of 5h57A

query
sites
5h57A

D

E

G

G

R

E

A

E

L

H

S

E

F

F

D

D

L

A

P

P

R

D

N

D

T

A

Q

Y

N

-

V

I

L

L

D

D

A

A

G

A

N

E

A

T

I

A

G

G

A

V

E

E

L

L

P

P

Y
|
Y

S

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

I

I

E

E

S

G

G

E

S

V

V

E

D

M

Q

D

S

S

D

N

G

H

S

A

F

L

L

E

D

D

D

Y

G

E

Q

V

Q

A

E

A

E

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

S

Y

Y

P

P

V

K

S

S

D

D

K

C

V

V

V

I

binding fe2/s2 (inorganic) cluster: Y38 (= Y302), C40 (= C304), R41 (≠ K305), G43 (= G307), C45 (= C309), C48 (= C312), C78 (= C342)

7s3dX Structure of photosystem i with bound ferredoxin from synechococcus sp. Pcc 7335 acclimated to far-red light (see paper)
38% identity, 24% coverage: 269:353/358 of query aligns to 4:89/97 of 7s3dX

query
sites
7s3dX

I

V

T

T

V

L

I

V

S

N

D

E

G

T

R

E

A

N

L

L

S

N

F

T

D

T

L

I

P

E

-

V

R

A

N

D

T

D

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

Q

G

G

A

I

E

D

L

L

P

P

Y
|
Y

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

L

I

T

E

E

G

G

E

T

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

C

V

T

V

V

L

M

binding fe2/s2 (inorganic) cluster: Y38 (= Y302), S39 (= S303), C40 (= C304), R41 (≠ K305), G43 (= G307), C45 (= C309), C48 (= C312), C78 (= C342)

7ylrA Structure of a bacteria protein
29% identity, 80% coverage: 67:354/358 of query aligns to 75:326/326 of 7ylrA

query
sites
7ylrA

E

E

L

Y

R

V

V

I

A
|
A

V
|
V

K
x
R

R

K

V

E

P

A

G

E

G
|
G

R
|
R

F

G

-
x
G

S
|
S

A

R

F

F

A

M

N

H

E

E

V

G

L

L

K

N

A

E

G

G

Q

D

Q

T

L

L

E

A

V

I

M

E

P

A

P

P

S

K

G

N

S

D

F

F

F

-

V

-

P

P

L

L

D

H

P

T

A

G

R

P

Q

G

G

G

N

S

Y

V

L

L

G

-

V

V

A

A

A

G

G

G

S

I

G

G

I

V

T
|
T

P

P

I

L

L

A

S

T

I

M

I

A

A

A

T

R

T

R

L

R

A

A

S

E

E

G

P

A

H

P

S

V

R

R

F

-

T

-

L

M

L

H

Y

Y

G

A

N

G

R

R

S

S

S

R

S

E

G

L

A

M

L

A

F

F

R

L

D

P

K

E

L

L

E

Q

D

A

L

L

K

L

N

G

R

D

Y

D

L

L

D

-

R

-

L

-

N

-

L

-

I

-

F

-

V

-

F

-

S

-

R

R

E

V

Q

H

Q

A

D

D

V

A

D

E

L

-

Y

A

N

G

G

A

R

P

I

L

D

D

A

I

D

D

K

A

C

-

G

-

Q

-

L

-

F

-

S

-

R

-

W

L

L

L

D

D

-

G

V

V

A

P

G

A

L

G

D

D

A

R

A

L

F

Y

I

V

C

C

G

G

P

P

Q

K

A

V

M

M

T

L

E

D

T

A

V

V

R

L

D

A

S

R

L

T

Q

Q

A

A

N

R

G

G

M

W

A

E

K

H

E

D

R

R

I

V

H

H

F

F

E
|
E

L

L

F
|
F

A

T

A
x
E

A

P

G

V

S

A

E

E

A

G

R

-

R

-

E

-

A

-

R

-

E

D

A

Q

A

P

R

F

Q

E

V

V

D

E

S

L

A

A

L

Q

S

S

H

-

I

-

T

-

V

-

I

-

S

-

D

-

G

-

R

-

A

G

L

Q

S

R

F

F

D

T

L

V

P

P

R

A

N

G

T

-

Q

Q

N

S

V

I

L

L

D

D

A

C

G

L

N

I

A

E

I

H

G

G

A

C

E

D

L

P

P

M

Y

F

S

D

C
|
C

K
|
K

A
x
R

G
|
G

V

E

C
|
C

S
x
G

T

V

C
|
C

K

A

C

V

R

P

V

V

I

L

E

E

G

G

E

E

V

I

E

D

M

-

D

H

S

R

N

D

H

Y

A

V

L

L

E

T

D

A

Y

R

E

E

V

K

A

A

A

Q

G

G

Y

N

V

V

L

M

S

Q

-

I

C
|
C

Q

I

T

S

Y

R

P

A

V

K

S

G

D

A

K

R

V

L

V

V

L

L

D

D

binding fe2/s2 (inorganic) cluster: C276 (= C304), K277 (= K305), G279 (= G307), C281 (= C309), G282 (≠ S310), C284 (= C312), C314 (= C342)
binding flavin mononucleotide: A79 (= A71), V80 (= V72), R81 (≠ K73), G86 (= G78), R87 (= R79), G89 (vs. gap), S90 (= S81), T131 (= T125), E229 (= E242), F231 (= F244), E233 (≠ A246), R278 (≠ A306)

Sites not aligning to the query:

binding flavin mononucleotide: 56, 57, 58, 59

6xtfD Crystal structure a thioredoxin reductase from gloeobacter violaceus bound to its electron donor (see paper)
36% identity, 24% coverage: 265:349/358 of query aligns to 1:84/96 of 6xtfD

query
sites
6xtfD

A

A

L

T

S

Y

H

K

I

V

T

T

V

L

I

V

S

T

D

P

G

S

R

G

A

K

L

K

S

E

F

I

D

D

L

C

P

P

R

S

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

R

I

Q

G

G

A

L

E

D

L

L

P

P

Y

F

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

M

I

V

E

S

G

G

E

T

V

I

E

D

M

Q

D

G

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

A

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

N

binding flavin-adenine dinucleotide: S38 (= S303), C39 (= C304)
binding fe2/s2 (inorganic) cluster: C39 (= C304), R40 (≠ K305), G42 (= G307), C44 (= C309), C47 (= C312), C77 (= C342)

1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
25% identity, 68% coverage: 2:246/358 of query aligns to 103:336/337 of 1krhA

query
sites
1krhA

S

T

Q

K

F

I

H

H

S

H

L

F

T

E

I

G

K

T

Q

L

V

A

R

R

N

V

E

E

T

N

R

L

D

S

A

D

V

S

S

T

I

I

A

T

F

F

D

D

V

I

P

Q

-

L

E

D

H

D

L

G

Q

Q

G

P

Q

D

F

I

R

H

F

F

T

L

Q

A

G

G

Q

Q

Y
|
Y

L

V

V

-

M

-

R

N

T

V

Q

T

L

L

D

P

N

G

E

T

E

T

V

E

R

T

R
|
R

S
|
S

Y
|
Y

S
|
S

I

F

C

S

S

S

A

Q

V

P

Q

G

D

N

G

R

E

L

L

T

R

G

V

F

A
x
V

V
|
V

K

R

R

N

V

V

P

P

G

Q

G
|
G

R
x
K

F
x
M

S
|
S

A

E

F

Y

A

L

N

S

E

V

V

Q

L

A

K

K

A

A

G

G

Q

D

Q

K

L

M

E

S

V

F

M

T

P

G

P

P

S

F

G

G

S

S

F

F

F

Y

V

L

P

-

L

R

D

D

P

V

A

K

R

R

Q

P

G

-

N

-

Y

V

L

L

G

M

V

L

A

A

A

G

G

G

S
x
T

G

G

I

I

T

A

P

P

I

F

L

L

S

S

I

M

I

L

A

Q

T

V

T

L

L

E

A

Q

S

K

E

G

P

S

H

E

S

H

R

P

F

V

T

R

L

L

L

V

Y

F

G

G

N

V

R

T

S

Q

S

D

S

C

G

D

A

L

L

V

F

A

R

L

D

E

K

Q

L

L

E

D

D

A

L

L

K

Q

N

Q

R

K

-

L

-

P

Y

W

L

F

D

E

R

Y

L

R

N

T

L

V

I

V

F

A

V

H

F

A

S

E

R

S

E

Q

Q

H

Q

E

D

R

V

K

D

G

L

Y

Y

V

N

T

G

G

R

H

I

I

D

E

A

Y

D

D

K

-

C

-

G

-

Q

-

L

-

F

-

S

-

R

-

W

W

L

L

D

N

V

G

A

G

G

E

L

V

D

D

A

-

A

V

F

Y

I

L

C
|
C

G

G

P

P

Q

V

A

P

M

M

T

V

E

E

T

A

V

V

R

R

D

S

S

W

L

L

Q

D

A

T

N

Q

G

G

M

I

A

Q

K

P

E

A

R

N

I

F

H

L

F

F

E
|
E

L

K

F
|
F

A
x
S

A
|
A

active site: C306 (= C216)
binding flavin-adenine dinucleotide: Y143 (= Y41), R155 (= R55), S156 (= S56), Y157 (= Y57), S158 (= S58), V171 (≠ A71), V172 (= V72), G178 (= G78), K179 (≠ R79), M180 (≠ F80), S181 (= S81), T219 (≠ S122), E332 (= E242), F334 (= F244), S335 (≠ A245), A336 (= A246)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 337
binding fe2/s2 (inorganic) cluster: 38, 39, 40, 41, 43, 45, 46, 48, 82

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
38% identity, 23% coverage: 269:352/358 of query aligns to 6:90/99 of P0A3C7

query
sites
P0A3C7

I

V

T

T

V

L

I

I

-

N

-

E

S

A

D

E

G

G

R

T

A

K

L

H

S

E

F

I

D

E

L

V

P

P

R

D

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

Q

G

G

A

Y

E

D

L

L

P

P

Y

F

S

S

C

C

K
x
R

A

A

G

G

V

A

C

C

S

S

T
|
T

C

C

K

A

C

G

R

K

V

L

I

V

E

S

G

G

E

T

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A
x
F

L

L

E

D

D
|
D

Y
x
D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L

L

S

T

C

C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

V

V

V

V

I

R43 (≠ K305) mutation R->A,E,H: No effect on electron transfer.
T49 (= T311) mutation to A: No effect on electron transfer.
F66 (≠ A328) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D331) mutation to K: Small effect on electron transfer.
D70 (≠ Y332) mutation to K: Small effect on electron transfer.

Sites not aligning to the query:

95 E→K: 20000-fold decrease in electron transfer.

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
38% identity, 23% coverage: 269:352/358 of query aligns to 5:89/98 of 1ewyC

query
sites
1ewyC

I

V

T

T

V

L

I

I

-

N

-

E

S

A

D

E

G

G

R

T

A

K

L

H

S

E

F

I

D

E

L

V

P

P

R

D

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

Q

G

G

A

Y

E

D

L

L

P

P

Y
x
F

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V
x
A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

L

I

V

E

S

G

G

E

T

V

V

E

D

M

Q

D

S

S

D

N

Q

H
x
S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L
|
L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

V

V

V

V

I

binding flavin-adenine dinucleotide: A45 (≠ V308), S64 (≠ H327)
binding fe2/s2 (inorganic) cluster: F39 (≠ Y302), S40 (= S303), C41 (= C304), R42 (≠ K305), G44 (= G307), A45 (≠ V308), C46 (= C309), C49 (= C312), L77 (= L340), C79 (= C342)

1czpA Anabaena pcc7119 [2fe-2s] ferredoxin in the reduced and oxixized state at 1.17 a (see paper)
38% identity, 23% coverage: 269:352/358 of query aligns to 5:89/98 of 1czpA

query
sites
1czpA

I

V

T

T

V

L

I

I

-

N

-

E

S

A

D

E

G

G

R

T

A

K

L

H

S

E

F

I

D

E

L

V

P

P

R

D

N

D

T

-

Q

E

N

Y

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

Q

G

G

A

Y

E

D

L

L

P

P

Y
x
F

S

S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

L

I

V

E

S

G

G

E

T

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

K

V

V

V

V

I

binding fe2/s2 (inorganic) cluster: F39 (≠ Y302), C41 (= C304), R42 (≠ K305), G44 (= G307), C46 (= C309), C49 (= C312), C79 (= C342)

P27320 Ferredoxin-1; Ferredoxin I from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 3 papers)
48% identity, 17% coverage: 289:349/358 of query aligns to 25:85/97 of P27320

query
sites
P27320

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

A

G

G

A

L

E

D

L

L

P

P

Y

Y

S

S

C
|
C

K

R

A

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

I

I

T

E

A

G

G

E

S

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

C40 (= C304) binding
C45 (= C309) binding
C48 (= C312) binding
C78 (= C342) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5aukA Crystal structure of the ga-substituted ferredoxin (see paper)
48% identity, 17% coverage: 289:349/358 of query aligns to 24:84/96 of 5aukA

query
sites
5aukA

V

I

L

L

D

D

A

A

G

A

N

E

A

E

I

A

G

G

A

L

E

D

L

L

P

P

Y
|
Y

S
|
S

C
|
C

K
x
R

A

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

K

A

C

G

R

K

V

I

I

T

E

A

G

G

E

S

V

V

E

D

M

Q

D

S

S

D

N

Q

H

S

A

F

L

L

E

D

D

D

Y

D

E

Q

V

I

A

E

A

A

G

G

Y

Y

V

V

L

L

S

T

C
|
C

Q

V

T

A

Y

Y

P

P

V

T

S

S

D

D

binding [2Ga-2S] cluster: Y37 (= Y302), S38 (= S303), C39 (= C304), R40 (≠ K305), G42 (= G307), C44 (= C309), C47 (= C312), C77 (= C342)

Query Sequence

>PP_3274 FitnessBrowser__Putida:PP_3274
MSQFHSLTIKQVRNETRDAVSIAFDVPEHLQGQFRFTQGQYLVMRTQLDNEEVRRSYSIC
SAVQDGELRVAVKRVPGGRFSAFANEVLKAGQQLEVMPPSGSFFVPLDPARQGNYLGVAA
GSGITPILSIIATTLASEPHSRFTLLYGNRSSSGALFRDKLEDLKNRYLDRLNLIFVFSR
EQQDVDLYNGRIDADKCGQLFSRWLDVAGLDAAFICGPQAMTETVRDSLQANGMAKERIH
FELFAAAGSEARREAREAARQVDSALSHITVISDGRALSFDLPRNTQNVLDAGNAIGAEL
PYSCKAGVCSTCKCRVIEGEVEMDSNHALEDYEVAAGYVLSCQTYPVSDKVVLDFDQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory