SitesBLAST
Comparing PP_3491 FitnessBrowser__Putida:PP_3491 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
34% identity, 97% coverage: 10:357/357 of query aligns to 3:348/362 of 3bptA
- active site: G67 (= G74), P84 (≠ E91), R88 (≠ V95), G115 (= G122), G118 (= G125), E138 (= E145), D146 (= D153)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G73), G67 (= G74), I69 (= I76), E90 (= E97), G114 (= G121), G115 (= G122), E138 (= E145), D146 (= D153), V147 (= V154)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ G32), L26 (= L33), A28 (= A35), G66 (= G73), G67 (= G74), I69 (= I76), P137 (= P144), I141 (= I148), L319 (≠ I328)
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 97% coverage: 9:355/357 of query aligns to 6:354/378 of Q9LKJ1
- G70 (= G74) mutation to S: Loss of activity.
- E142 (= E145) mutation to A: Loss of activity.
- D150 (= D153) mutation to G: Reduced activity.
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
37% identity, 96% coverage: 11:354/357 of query aligns to 1:328/340 of 4hdtA
- active site: G64 (= G74), I69 (≠ L79), W84 (≠ F94), Y88 (= Y98), G112 (= G122), G115 (= G125), E135 (= E145), P142 (= P152), D143 (= D153), R283 (≠ A305)
- binding zinc ion: H28 (≠ L38), E42 (≠ A52), E57 (≠ P67), E79 (≠ L89), H93 (≠ V103), H185 (≠ S195)
Sites not aligning to the query:
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 52% coverage: 25:208/357 of query aligns to 17:194/259 of 5zaiC
- active site: A65 (≠ G74), F70 (≠ L79), S82 (≠ E96), R86 (≠ L100), G110 (= G122), E113 (≠ G125), P132 (= P144), E133 (= E145), I138 (≠ Y150), P140 (= P152), G141 (≠ D153)
- binding coenzyme a: K24 (≠ G32), L25 (= L33), A63 (= A72), G64 (= G73), A65 (≠ G74), D66 (= D75), I67 (= I76), P132 (= P144), R166 (≠ Q177)
Sites not aligning to the query:
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
34% identity, 48% coverage: 19:188/357 of query aligns to 14:178/260 of 1dubA
- active site: A68 (≠ G74), M73 (≠ L79), S83 (≠ A99), L87 (≠ V103), G111 (= G122), E114 (≠ G125), P133 (= P144), E134 (= E145), T139 (≠ Y150), P141 (= P152), G142 (≠ D153)
- binding acetoacetyl-coenzyme a: K26 (≠ A31), A27 (≠ G32), L28 (= L33), A30 (= A35), A66 (= A72), A68 (≠ G74), D69 (= D75), I70 (= I76), Y107 (≠ F118), G110 (= G121), G111 (= G122), E114 (≠ G125), P133 (= P144), E134 (= E145), L137 (≠ I148), G142 (≠ D153)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
35% identity, 48% coverage: 19:188/357 of query aligns to 13:172/254 of 2dubA
- active site: A67 (≠ G74), M72 (≠ L79), S82 (≠ A99), G105 (= G122), E108 (≠ G125), P127 (= P144), E128 (= E145), T133 (≠ Y150), P135 (= P152), G136 (≠ D153)
- binding octanoyl-coenzyme a: K25 (≠ A31), A26 (≠ G32), L27 (= L33), A29 (= A35), A65 (= A72), A67 (≠ G74), D68 (= D75), I69 (= I76), K70 (≠ R77), G105 (= G122), E108 (≠ G125), P127 (= P144), E128 (= E145), G136 (≠ D153), A137 (≠ V154)
Sites not aligning to the query:
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
34% identity, 48% coverage: 19:188/357 of query aligns to 12:176/258 of 1ey3A
- active site: A66 (≠ G74), M71 (≠ L79), S81 (≠ A99), L85 (≠ V103), G109 (= G122), E112 (≠ G125), P131 (= P144), E132 (= E145), T137 (≠ Y150), P139 (= P152), G140 (≠ D153)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ A31), L26 (= L33), A28 (= A35), A64 (= A72), G65 (= G73), A66 (≠ G74), D67 (= D75), I68 (= I76), L85 (≠ V103), W88 (vs. gap), G109 (= G122), P131 (= P144), L135 (≠ I148), G140 (≠ D153)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
32% identity, 50% coverage: 20:198/357 of query aligns to 45:218/290 of P14604
- E144 (≠ G125) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E145) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
33% identity, 50% coverage: 12:188/357 of query aligns to 4:178/260 of 2hw5C
- active site: A68 (≠ G74), M73 (≠ L79), S83 (≠ A99), L87 (≠ V103), G111 (= G122), E114 (≠ G125), P133 (= P144), E134 (= E145), T139 (≠ Y150), P141 (= P152), G142 (≠ D153)
- binding crotonyl coenzyme a: K26 (≠ A31), A27 (≠ G32), L28 (= L33), A30 (= A35), K62 (= K68), I70 (= I76), F109 (≠ L120)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
34% identity, 48% coverage: 19:188/357 of query aligns to 14:176/258 of 1mj3A
- active site: A68 (≠ G74), M73 (≠ L79), S83 (≠ F94), L85 (≠ E96), G109 (= G122), E112 (≠ G125), P131 (= P144), E132 (= E145), T137 (≠ Y150), P139 (= P152), G140 (≠ D153)
- binding hexanoyl-coenzyme a: K26 (≠ A31), A27 (≠ G32), L28 (= L33), A30 (= A35), A66 (= A72), G67 (= G73), A68 (≠ G74), D69 (= D75), I70 (= I76), G109 (= G122), P131 (= P144), E132 (= E145), L135 (≠ I148), G140 (≠ D153)
Sites not aligning to the query:
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
30% identity, 52% coverage: 12:198/357 of query aligns to 4:185/255 of 3q0jC
- active site: A65 (≠ G74), M70 (≠ L79), T80 (≠ F94), F84 (≠ Y98), G108 (= G122), E111 (≠ G125), P130 (= P144), E131 (= E145), V136 (≠ Y150), P138 (= P152), G139 (≠ D153)
- binding acetoacetyl-coenzyme a: Q23 (≠ A31), A24 (≠ G32), L25 (= L33), A27 (= A35), A63 (= A72), G64 (= G73), A65 (≠ G74), D66 (= D75), I67 (= I76), K68 (≠ R77), M70 (≠ L79), F84 (≠ Y98), G107 (= G121), G108 (= G122), E111 (≠ G125), P130 (= P144), E131 (= E145), P138 (= P152), G139 (≠ D153), M140 (≠ V154)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 52% coverage: 12:198/357 of query aligns to 4:185/255 of 3q0gC
- active site: A65 (≠ G74), M70 (≠ L79), T80 (≠ F94), F84 (≠ Y98), G108 (= G122), E111 (≠ G125), P130 (= P144), E131 (= E145), V136 (≠ Y150), P138 (= P152), G139 (≠ D153)
- binding coenzyme a: L25 (= L33), A63 (= A72), I67 (= I76), K68 (≠ R77), Y104 (≠ F118), P130 (= P144), E131 (= E145), L134 (≠ I148)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
30% identity, 52% coverage: 12:198/357 of query aligns to 3:184/256 of 3h81A
- active site: A64 (≠ G74), M69 (≠ L79), T79 (≠ F94), F83 (≠ Y98), G107 (= G122), E110 (≠ G125), P129 (= P144), E130 (= E145), V135 (≠ Y150), P137 (= P152), G138 (≠ D153)
Sites not aligning to the query:
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
31% identity, 52% coverage: 12:198/357 of query aligns to 3:180/250 of 3q0gD
- active site: A64 (≠ G74), M69 (≠ L79), T75 (≠ H90), F79 (= F94), G103 (= G122), E106 (≠ G125), P125 (= P144), E126 (= E145), V131 (≠ Y150), P133 (= P152), G134 (≠ D153)
Sites not aligning to the query:
Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; L-bifunctional protein; LBP; Multifunctional enzyme 1; MFE1; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human) (see 5 papers)
30% identity, 57% coverage: 20:221/357 of query aligns to 9:209/723 of Q08426
- V40 (≠ A52) to G: in dbSNP:rs1062551
- I41 (≠ D53) to R: in dbSNP:rs1062552
- T75 (= T88) to I: in dbSNP:rs1062553
- K165 (≠ Y185) modified: N6-acetyllysine; alternate; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-171; Q-346 and Q-584.
- K171 (≠ W191) modified: N6-acetyllysine; mutation to Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-346 and Q-584.
Sites not aligning to the query:
- 3 E → K: in FRTS3; the mutant is mistargeted to mitochondria; results in impaired mitochondrial oxidative phosphorylation and defects in the transport of fluids across the epithelium of renal proximal tubular cells; dbSNP:rs398124646
- 274 A → T: in dbSNP:rs2302819
- 325 A → G: in dbSNP:rs1062555
- 346 modified: N6-acetyllysine; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-584.
- 584 modified: N6-acetyllysine; alternate; K→Q: Greatly reduced acetylation and insensitive to treatment with TSA and NAM; when associated with Q-165; Q-171 and Q-346.
- 598 K → T: in dbSNP:rs1042437
- 606 T → P: in dbSNP:rs1042438
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
32% identity, 55% coverage: 15:209/357 of query aligns to 11:203/266 of O53561
- K135 (≠ R140) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 140:147, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ G147) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
32% identity, 56% coverage: 17:215/357 of query aligns to 10:206/261 of 5jbxB
- active site: A67 (≠ G74), R72 (vs. gap), L84 (vs. gap), R88 (≠ H90), G112 (= G122), E115 (≠ G125), T134 (≠ P144), E135 (= E145), I140 (≠ Y150), P142 (= P152), G143 (≠ D153)
- binding coenzyme a: S24 (≠ A31), R25 (≠ G32), R26 (≠ L33), A28 (= A35), A65 (= A72), D68 (= D75), L69 (≠ I76), K70 (≠ R77), L110 (= L120), G111 (= G121), T134 (≠ P144), E135 (= E145), L138 (≠ I148), R168 (≠ Q177)
Sites not aligning to the query:
6z1pBI mS93 (see paper)
28% identity, 50% coverage: 13:189/357 of query aligns to 23:201/1413 of 6z1pBI
- active site: T85 (≠ G74), S134 (≠ G122), E157 (= E145), D165 (= D153)
- binding : Y41 (≠ A31), K42 (≠ G32), Q43 (≠ L33), T45 (≠ A35), D47 (≠ T37), H49 (≠ D39), K83 (≠ A72), T85 (≠ G74), D86 (= D75), F87 (≠ I76), K88 (≠ R77), K92 (≠ D81), L130 (≠ F118), K152 (≠ R140)
Sites not aligning to the query:
6z5oAAA Peroxisomal bifunctional enzyme (see paper)
31% identity, 59% coverage: 13:221/357 of query aligns to 15:214/716 of 6z5oAAA
- active site: A67 (≠ G74), F72 (≠ Y80), G82 (≠ H90), G106 (= G122), E109 (≠ G125), P128 (= P144), E129 (= E145), G137 (≠ D153)
- binding coenzyme a: P26 (= P30), V27 (≠ L33), A65 (= A72), D68 (= D75), I69 (= I76), P128 (= P144), Y162 (≠ Q177)
- binding nicotinamide: A67 (≠ G74), E109 (≠ G125), E129 (= E145), P136 (= P152)
Sites not aligning to the query:
- active site: 255, 409, 430, 442, 480
- binding coenzyme a: 277, 281
- binding nicotinamide-adenine-dinucleotide: 309, 311, 312, 313, 332, 333, 337, 379, 380, 381, 382, 387, 407, 409, 430
- binding nicotinamide: 261
6zibAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with acetoacetyl-coa and nadh'
31% identity, 59% coverage: 13:221/357 of query aligns to 14:213/723 of 6zibAAA
- active site: A66 (≠ G74), F71 (≠ Y80), G81 (≠ H90), G105 (= G122), E108 (≠ G125), P127 (= P144), E128 (= E145), G136 (≠ D153)
- binding acetoacetyl-coenzyme a: P25 (= P30), V26 (≠ L33), A64 (= A72), G65 (= G73), A66 (≠ G74), D67 (= D75), I68 (= I76), G104 (= G121), G105 (= G122), E128 (= E145), Y161 (≠ Q177)
Sites not aligning to the query:
- active site: 254, 413, 434, 446, 484
- binding 1,4-dihydronicotinamide adenine dinucleotide: 310, 311, 312, 331, 332, 336, 383, 384, 385, 386, 411, 434
Query Sequence
>PP_3491 FitnessBrowser__Putida:PP_3491
MTAHAHTPAATDHVLAEVRNQIGHLTLNRPAGLNALTLDMVRSLRQHLDQWADNPQVRAV
VLRGEGPKGFCAGGDIRSLYDSFKAGDTLHETFFVEEYALDLVIHRYRKPILVVMDGFTL
GGGMGLAQGCDLRVVTERSRLGMPEVGIGYFPDVGGSYFLSRLPGELGIYLGVSGAQIQA
ADALYCGLADWYLASDQLAALDQGLDQLDFSDHPLKDLQNLLARLGTQVLDDAPLEKLRP
AIDHFFALPDLPAIVEQLRAVSIGDSHAWAVATADQLETRSPLAMAVTLEMLRRGRELGI
DDCFAMELHLDRQWFQHGDIIEGVRALIIDKDKQPRWNPPTLAALQSQRVEQFFEGL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory