SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_3553 PP_3553 AMP-binding domain protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 98% coverage: 9:538/540 of query aligns to 9:535/541 of Q5SKN9

query
sites
Q5SKN9

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T184 (= T189) binding
W234 (= W237) binding
G323 (≠ T325) binding
T327 (= T329) binding
E328 (= E330) binding
D418 (= D421) binding
K435 (= K438) binding
K439 (≠ I442) binding
W444 (≠ N447) binding

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
38% identity, 96% coverage: 9:529/540 of query aligns to 2:502/510 of 1v26B

query
sites
1v26B

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active site: T177 (= T189), H197 (≠ N209), H223 (= H233), T320 (= T329), E321 (= E330), K432 (≠ I442), W437 (≠ N447)
binding adenosine monophosphate: G295 (= G304), S296 (≠ A305), A297 (= A306), Q315 (≠ H324), G316 (≠ T325), Y317 (= Y326), G318 (= G327), L319 (= L328), T320 (= T329), D411 (= D421), I423 (= I433), K428 (= K438), K432 (≠ I442), W437 (≠ N447)
binding magnesium ion: T177 (= T189), E321 (= E330)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
39% identity, 89% coverage: 9:486/540 of query aligns to 2:466/491 of 1v25A

query
sites
1v25A

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T

G

G

T

T

G

G

N

L

P

P

K

K

G

G

V

V

Y

Y

H

S

H

H

R

R

G

A

A

L

Y

V

L

L

N
x
H

A

S

L

L

G

A

N

A

Q

S

M

L

T

V

-

D

-

G

W

T

A

A

M

L

G

S

H

E

R

K

P

D

V

V

Y

V

L

L

W

P

T

V

L

V

P

P

M

M

F
|
F

H
|
H

C
x
V

N

N

G

A

W
|
W

C

C

Y

L

P

P

W

Y

T

A

I

A

T

T

A

L

L

V

A

G

G

A

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K

H

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V

F

L

L

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P

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D

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A

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L

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K

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L

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M

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V

V

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G
|
G

A
x
S

A
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A

P

A

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K

S

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L

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I

G

A

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F

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E

Q

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M

M

G

G

I

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K

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H
x
Q

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x
G

Y
|
Y

G
|
G

L
|
L

T
|
T

E
|
E

V

T

Y

-

G

S

P

P

V

V

T

V

V

V

C

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A

N

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F

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V

H

K

D

S

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H

W

L

D

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A

S

L

L

S

S

L

E

E

E

R

K

A

L

R

T

I

L

K

K

S

A

R

K

Q

T

G

G

V

L

R

P

Y

I

P

P

T

-

L

L

D

V

G

R

L

L

M

R

V

V

A

A

D

D

P

E

Q

E

T

G

L

-

Q

R

P

P

V

V

P

P

R

K

D

D

G

G

D

K

T

A

L

L

G

G

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E

I

V

F

Q

M

L

R

K

G

G

N

P

T

W

V

I

M

T

K

G

G

G

Y

Y

L

Y

K

G

N

N

P

E

E

E

A

A

T

T

A

R

E

S

A

A

F

L

R

T

-

P

G

D

G

G

W

F

F

F

H

R

T

T

G

G

D
|
D

L

I

A

A

V

V

W

W

H

D

A

E

D

E

G

G

Y

Y

I

V

E

E

I
|
I

K

K

D

D

R
|
R

L

L

K
|
K

D

D

I

L

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

T

S

I

V

E

D

V

L

E

E

D

N

A

-

L

-

Y

-

K

-

H

-

S

-

A

-

V

-

L

-

E

-

A

A

V

V

A

A

R

I

P

P

D

H

E

P

K

K

W

W

G

Q

E

E

T

R

P

P

C

L

A

A

F

V

V

V

A

G

L

F

active site: T177 (= T189), H197 (≠ N209), H223 (= H233), T320 (= T329), E321 (= E330), K432 (≠ I442), W437 (≠ N447)
binding phosphoaminophosphonic acid-adenylate ester: F222 (= F232), H223 (= H233), V224 (≠ C234), W227 (= W237), G295 (= G304), S296 (≠ A305), A297 (= A306), Q315 (≠ H324), G316 (≠ T325), Y317 (= Y326), G318 (= G327), L319 (= L328), T320 (= T329), D411 (= D421), I423 (= I433), R426 (= R436), K428 (= K438), K432 (≠ I442), W437 (≠ N447)
binding magnesium ion: T177 (= T189), E321 (= E330)

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
32% identity, 96% coverage: 22:538/540 of query aligns to 14:534/538 of 6ijbB

query
sites
6ijbB

S

S

F

L

I

I

E

D

R

H

T

A

A

A

A

R

V

Y

Y

H

G

G

N

Q

Y

T

P

E

A

I

V

V

-

S

-

V

-

E

I

T

H

D

G

G

A

T

I

V

R

T

R

R

-

T

N

N

W

W

Q

G

E

E

T

I

Y

A

Q

A

R

N

C

A

R

R

L

M

A

G

S

S

A

A

L

L

A

T

G

K

R

L

G

G

I

L

G

Q

R

P

G

Q

D

D

T

R

V

I

A

G

V

T

M

L

L

A

P

W

N

N

T

N

P

R

T

R

M

H

L

L

E

E

A

I

H

Y

F

Y

G

A

V

A

P

S

M

G

T

A

G

G

A

F

V

V

L

C

N

H

T

T

L

I

N

N

V

P

R

R

L

L

D

F

A

P

E

E

A

Q

I

L

A

V

F

Y

M

I

L

I

Q

N

H

H

G

A

E

Q

A

D

K

R

V

V

L

L

I

F

T

F

D

D

R

A

E

T

F

F

H

L

-

P

-

L

-

V

A

A

V

A

I

I

E

R

G

D

A

Q

L

L

A

T

L

E

L

V

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K

H

H

P

F

P

V

L

L

V

M

V

G

D

P

V

R

D

N

D

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P

-

E

-

Y

-

G

-

E

-

G

-

R

D

A

A

V

L

S

Q

Q

Q

L

I

D

P

-

G

-

L

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E

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F

Y

Y

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D

A

E

L

L

L

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N

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G

D

D

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T

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F

F

A

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W

-

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W

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D

F

D

D

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W

N

Q

T

A

A

I

S

S

S

L

L

N

C

Y

Y

T
|
T

S

S

G

G

T

T

G

G

N

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

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R

G

S

A

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Y

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L

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x
H

A

S

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F

G

A

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S

Q

N

M

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W

D

A

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M

I

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G

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Y

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A

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M

-

D

V

V

Y

V

L

M

W

P

T

V

L

V

P

P

M

M

F

F

H
|
H

C

V

N

N

G

A

W
|
W

C

G

Y

S

P

P

W

Y

T

G

I

-

T

C

A

A

L

M

A

S

G

G

T

A

H

Q

V

M

F

V

L

L

R

P

R

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V

P

D

D

-

L

-

H

P

G

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K

A

I

L

L

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N

L

L

I

I

R

D

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H

Y

K

G

V

V

S

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H

L

L

A

C

M

G

G

A

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P

P

I

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V

I

L

W

N

Q

A

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L

L

V

L

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A

M

H

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A

E

A

A

K

A

C

K

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A

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A

K

I

L

E

E

H

S

P

L

V

E

Q

R

A

T

M

V

V

I

A
x
G

G
|
G

A
|
A

-

C

P

P

A

S

K

M

V

I

I

A

G

T

A

F

V

R

E

E

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K

M

Y

G

G

I

V

K

D

V

T

T

V

H
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H

T
x
A

Y
x
W

G
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G

L
x
M

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x
S

E
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E

V

M

Y

S

G
x
P

P
x
L

V

G

T

T

V

A

C

N

A

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A

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K

W

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K

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L

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P

L

I

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A

H

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L

K

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Q
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Q

G

G

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R

R

Y

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P

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F

L

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D

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G

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L

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D

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D

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G

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T

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G

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I

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F

M

M

V

R

R

G

G

N

H

T

W

V

V

M

L

K

D

G

S

Y

Y

L

F

K

Q

N

L

P

K

E

D

A

Q

T

-

A

-

E

E

A

L

F

L

R

Q

G

D

G

G

W

W

F

F

H

A

T

T

G

G

D
|
D

L

V

A

A

V

T

W

L

H

D

A

P

D

D

G

G

Y

Y

I

M

E

T

I
|
I

K

R

D

D

R
|
R

L

S

K

K

D

D

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

T

S

I

V

E

E

V

L

E

E

D

N

A

I

L

A

Y

V

K

A

H

H

S

P

A

K

V

L

L

A

E

T

A

A

V

V

V

I

A

G

R

V

P

P

D

H

E

P

K

K

W

W

G

D

E

E

T

R

P

P

C

L

A

-

F

L

V

V

A

A

L

V

K

K

P

A

G

E

R

G

E

E

D

D

T

P

R

S

E

E

A

A

D

E

I

L

T

L

S

E

W

F

C

F

R

D

E

G

H

K

L

I

A

A

G

K

F

W

K

Q

V

V

P

P

K

D

T

K

V

V

F

F

G

V

E

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
x
A

I

V

Q

L

K

K

Y

R

V

K

L

L

R

R

D

D

R

E

A

F

K

K

active site: T185 (= T189), H205 (≠ N209), H231 (= H233), S329 (≠ T329), E330 (= E330), K438 (≠ I442), W443 (≠ N447), A523 (≠ K527)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W237), G303 (= G304), A325 (≠ T325), W326 (≠ Y326), G327 (= G327), M328 (≠ L328), P333 (≠ G333), L334 (≠ P334)
binding adenosine monophosphate: T185 (= T189), H231 (= H233), G302 (≠ A303), G303 (= G304), A304 (= A305), A305 (= A306), H324 (= H324), A325 (≠ T325), W326 (≠ Y326), G327 (= G327), M328 (≠ L328), S329 (≠ T329), Q359 (= Q359), D417 (= D421), I429 (= I433), R432 (= R436)

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
32% identity, 96% coverage: 22:538/540 of query aligns to 14:531/533 of 6ihkB

query
sites
6ihkB

S

S

F

L

I

I

E

D

R

H

T

A

A

A

A

R

V

Y

Y

H

G

G

N

Q

Y

T

P

E

A

I

V

V

-

S

-

V

-

E

I

T

H

D

G

G

A

T

I

V

R

T

R

R

-

T

N

N

W

W

Q

G

E

E

T

I

Y

A

Q

A

R

N

C

A

R

R

L

M

A

G

S

S

A

A

L

L

A

T

G

K

R

L

G

G

I

L

G

Q

R

P

G

Q

D

D

T

R

V

I

A

G

V

T

M

L

L

A

P

W

N

N

T

N

P

R

T

R

M

H

L

L

E

E

A

I

H

Y

F

Y

G

A

V

A

P

S

M

G

T

A

G

G

A

F

V

V

L

C

N

H

T

T

L

I

N

N

V

P

R

R

L

L

D

F

A

P

E

E

A

Q

I

L

A

V

F

Y

M

I

L

I

Q

N

H

H

G

A

E

Q

A

D

K

R

V

V

L

L

I

F

T

F

D

D

R

A

E

T

F

F

H

L

-

P

-

L

-

V

A

A

V

A

I

I

E

R

G

D

A

Q

L

L

A

T

L

E

L

V

E

K

H

H

P

F

P

V

L

L

V

M

V

G

D

P

V

R

D

N

D

E

P

-

E

-

Y

-

G

-

E

-

G

-

R

D

A

A

V

L

S

Q

Q

Q

L

I

D

P

-

G

-

L

-

E

-

F

Y

Y

E

D

A

E

L

L

L

I

N

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E

T

G

G

D

D

P

T

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D

F

F

A

-

W

-

E

E

W

W

P

P

-

V

D

F

D

D

E

E

W

N

Q

T

A

A

I

S

S

S

L

L

N

C

Y

Y

T
|
T

S

S

G

G

T

-

G

-

N

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

N
x
H

A

S

L

F

G

A

N

S

Q

N

M

T

T

R

W

D

A

V

M

I

G

G

H

Y

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S

P

A

-

M

-

D

V

V

Y

V

L

M

W

P

T

V

L

V

P

P

M

M

F

F

H
|
H

C

V

N

N

G

A

W

W

C

G

Y

S

P

P

W

Y

T

G

I

-

T

C

A

A

L

M

A

S

G

G

T

A

H

Q

V

M

F

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L

L

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P

R

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D

D

-

L

-

H

P

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A

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L

L

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L

L

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L

L

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M

G

G

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P

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W

N

Q

A

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L

L

V

L

N

A

M

H

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A

E

A

A

K

A

C

K

G

A

T

A

K

I

L

E

E

H

S

P

L

V

E

Q

R

A

T

M

V

V

I

A
x
G

G
|
G

A
|
A

-

C

P

P

A

S

K

M

V

I

I

A

G

T

A

F

V

R

E

E

Q

K

M

Y

G

G

I

V

K

D

V

T

T

V

H
|
H

T
x
A

Y
x
W

G
|
G

L
x
M

T
x
S

E
|
E

V

M

Y

S

G

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P

L

V

G

T

T

V

A

C

N

A

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W

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L

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K

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Q
|
Q

G

G

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-

R

R

Y

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P

P

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F

L

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D

V

G

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L

L

M

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Q

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D

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G

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M

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M

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Y

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A

-

E

E

A

L

F

L

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Q

G

D

G

G

W

W

F

F

H

A

T

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G

G

D
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active site: T185 (= T189), H202 (≠ N209), H228 (= H233), S326 (≠ T329), E327 (= E330), K435 (≠ I442), W440 (≠ N447), K520 (= K527)
binding adenosine-5'-diphosphate: T185 (= T189), H228 (= H233), G299 (≠ A303), G300 (= G304), A301 (= A305), A302 (= A306), H321 (= H324), A322 (≠ T325), W323 (≠ Y326), G324 (= G327), M325 (≠ L328), S326 (≠ T329), Q356 (= Q359), D414 (= D421), I426 (= I433), R429 (= R436), K520 (= K527)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
32% identity, 92% coverage: 42:539/540 of query aligns to 28:499/503 of P9WQ37

query
sites
P9WQ37

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K172 (= K197) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ G220) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (= R222) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C234) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G236) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ P240) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ K269) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G327) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W416) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D421) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R436) mutation to A: Reduction of binding affinity for fatty acids.
S404 (= S443) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G445) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K527) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
31% identity, 92% coverage: 42:539/540 of query aligns to 31:499/502 of 3r44A

query
sites
3r44A

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active site: T167 (= T192), A184 (≠ N209), H208 (= H233), T304 (= T329), E305 (= E330), I403 (= I442), N408 (= N447), K487 (= K527)
binding histidine: H178 (= H203), E179 (≠ R204), H182 (≠ Y207)

Sites not aligning to the query:

binding histidine: 4, 5, 6, 7

5gtdA O-succinylbenzoate coa synthetase (mene) from bacillus subtilis in complex with the acyl-adenylate intermediate osb-amp (see paper)
28% identity, 96% coverage: 18:538/540 of query aligns to 14:481/484 of 5gtdA

query
sites
5gtdA

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active site: T151 (= T189), S171 (≠ N209), H195 (= H233), T288 (= T329), E289 (= E330)
binding adenosine-5'-monophosphate: H195 (= H233), G263 (= G304), G264 (≠ A305), P265 (≠ A306), S284 (≠ T325), Y285 (= Y326), G286 (= G327), M287 (≠ L328), T288 (= T329), D366 (= D421), V378 (≠ I433), R381 (= R436), K470 (= K527)
binding magnesium ion: F314 (≠ P364), S315 (≠ T365)
binding 2-succinylbenzoate: L191 (= L229), H195 (= H233), I196 (≠ C234), S197 (≠ N235), S236 (≠ C274), A237 (≠ G275), L260 (≠ M301), G262 (≠ A303), G263 (= G304), G286 (= G327), M287 (≠ L328), T288 (= T329), S292 (≠ G333), Q293 (≠ P334)

5x8fB Ternary complex structure of a double mutant i454ra456k of o- succinylbenzoate coa synthetase (mene) from bacillus subtilis bound with amp and its product analogue osb-ncoa at 1.76 angstrom (see paper)
28% identity, 96% coverage: 18:538/540 of query aligns to 14:481/485 of 5x8fB

query
sites
5x8fB

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