SitesBLAST
Comparing PP_3622 FitnessBrowser__Putida:PP_3622 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8gy3C Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
29% identity, 90% coverage: 68:745/751 of query aligns to 20:718/732 of 8gy3C
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): M38 (≠ G86), G39 (= G87), Q40 (= Q88), H41 (≠ G89), V42 (≠ P90), A45 (= A93), G79 (= G131), G80 (= G132), S81 (= S133), S83 (= S135), V84 (= V136), G374 (≠ F423), F375 (= F424), L379 (≠ F428), L499 (≠ W541), R500 (= R542), V624 (= V651), D625 (≠ N652), Q632 (= Q659), T687 (≠ G714), G688 (= G715), L689 (≠ I716), G690 (= G717), E691 (= E718)
1ffvB Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
27% identity, 46% coverage: 231:572/751 of query aligns to 27:409/797 of 1ffvB
- active site: Q231 (= Q393), V266 (≠ F428), P343 (vs. gap), I349 (≠ L515), R378 (≠ W541), C379 (≠ R542)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): G260 (= G422), G261 (≠ F423), F262 (= F424), G263 (= G425), A376 (≠ G539), R378 (≠ W541), C379 (≠ R542)
Sites not aligning to the query:
- active site: 751, 752
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 516, 517, 518, 520, 523, 556, 557, 558, 560, 561, 674, 678, 683, 686, 747, 748, 749, 750, 751
1ffuB Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
27% identity, 46% coverage: 231:572/751 of query aligns to 27:409/797 of 1ffuB
Sites not aligning to the query:
- active site: 751, 752
- binding cytidine-5'-diphosphate: 518, 520, 523, 558, 560, 561, 674, 676, 678, 683, 747, 748, 749, 750
P19913 Carbon monoxide dehydrogenase large chain; CO dehydrogenase subunit L; CO-DH L; EC 1.2.5.3 from Hydrogenophaga pseudoflava (Pseudomonas carboxydoflava) (see paper)
27% identity, 46% coverage: 231:572/751 of query aligns to 33:415/803 of P19913
- R384 (≠ W541) modified: 4-hydroxyarginine
1t3qB Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
23% identity, 48% coverage: 214:572/751 of query aligns to 12:401/786 of 1t3qB
Sites not aligning to the query:
- active site: 743, 744
- binding pterin cytosine dinucleotide: 506, 507, 508, 510, 513, 545, 547, 549, 550, 666, 670, 674, 675, 678, 739, 740, 741, 742
1rm6A Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
33% identity, 20% coverage: 598:746/751 of query aligns to 587:746/761 of 1rm6A
- active site: E718 (= E718), G719 (≠ P719)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): L646 (≠ V651), N647 (= N652), V651 (≠ I656), Q654 (= Q659), K714 (vs. gap), E715 (vs. gap), A716 (vs. gap), S717 (vs. gap), E718 (= E718)
Sites not aligning to the query:
- active site: 206, 241, 318, 322, 350
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 235, 236, 237, 238, 350, 473, 474, 475, 476, 513, 514, 515, 517, 518
3hrdE Crystal structure of nicotinate dehydrogenase (see paper)
23% identity, 48% coverage: 213:573/751 of query aligns to 4:381/420 of 3hrdE
- active site: Q207 (= Q393), L242 (≠ F428), R318 (≠ P509), H322 (≠ E513), R350 (= R542)
- binding calcium ion: T206 (≠ N392), N208 (≠ A394), D212 (≠ F398), K241 (≠ H427), L242 (≠ F428), D243 (≠ S432)
- binding pterin cytosine dinucleotide: G237 (≠ F423), F238 (= F424), R350 (= R542)
- binding selenium atom: F238 (= F424), A348 (≠ Y540), F349 (≠ W541), R350 (= R542)
3hrdA Crystal structure of nicotinate dehydrogenase (see paper)
23% identity, 48% coverage: 213:573/751 of query aligns to 4:381/420 of 3hrdA
- active site: Q207 (= Q393), L242 (≠ F428), R318 (≠ P509), H322 (≠ E513), R350 (= R542)
- binding pterin cytosine dinucleotide: G236 (= G422), G237 (≠ F423), F238 (= F424), R350 (= R542)
- binding magnesium ion: T206 (≠ N392), N208 (≠ A394), D212 (≠ F398), K241 (≠ H427), L242 (≠ F428), D243 (≠ S432), T305 (= T496), Y308 (≠ L499), A309 (= A500), S346 (≠ L538)
- binding nicotinic acid: A314 (≠ E505), R318 (≠ P509), F352 (≠ V544)
- binding selenium atom: F238 (= F424), G239 (= G425), A348 (≠ Y540), F349 (≠ W541), R350 (= R542)
O33819 4-hydroxybenzoyl-CoA reductase subunit alpha; 4-HBCR subunit alpha; EC 1.1.7.1 from Thauera aromatica (see paper)
33% identity, 20% coverage: 598:746/751 of query aligns to 595:754/769 of O33819
- VGKALN 650:655 (≠ PGSIVN 647:652) binding Mo-molybdopterin cytosine dinucleotide
- KEAS 722:725 (vs. gap) binding Mo-molybdopterin cytosine dinucleotide
Sites not aligning to the query:
- 214 binding Mo-molybdopterin cytosine dinucleotide
- 244:245 binding Mo-molybdopterin cytosine dinucleotide
- 522:526 binding Mo-molybdopterin cytosine dinucleotide
Q0QLF2 Nicotinate dehydrogenase large molybdopterin subunit; NDH; Nicotinic acid hydroxylase large molybdopterin subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see 2 papers)
23% identity, 48% coverage: 213:573/751 of query aligns to 5:382/425 of Q0QLF2
- Q208 (= Q393) binding Se-Mo-molybdopterin cytosine dinucleotide
- GFG 238:240 (≠ FFG 423:425) binding Se-Mo-molybdopterin cytosine dinucleotide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2:425 modified: mature protein, Nicotinate dehydrogenase large molybdopterin subunit
5y6qC Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
26% identity, 34% coverage: 344:599/751 of query aligns to 156:401/748 of 5y6qC