SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_4063 FitnessBrowser__Putida:PP_4063 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q4WR83 Acyl-CoA ligase sidI; Siderophore biosynthesis protein I; EC 6.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
38% identity, 98% coverage: 5:552/560 of query aligns to 24:575/590 of Q4WR83

query
sites
Q4WR83

S

S

Y

I

T

V

R

E

G

G

R

P

Q

T

D

Q

R

P

P

E

L

L

T

D

Q

I

T

T

I

L

G

G

Q

E

A

L

F

L

D

T

A

L

T

Q

V

S

A

L

R

Q

C

Y

C

G

D

D

S

Y

E

E

A

C

L

L

V

V

S

F

R

P

H

W

Q

T

G

G

L

A

R

R

Y

W

S

T

W

Y

R

T

Q

D

L

L

A

K

E

D

Q

E

V

A

E

D

I

R

Y

V

A

A

R

R

A

G

L

L

I

L

A

A

L

M

G

G

V

I

N

Q

T

K

G

G

D

D

R

R

V

I

G

G

I

I

W

M

S

A

P

G

N

N

C

C

A

E

Q

Q

W

Y

C

I

I

S

L

V

Q

F

L

F

A

A

S

A

A

A

K

R

V

V

G

G

A

A

I

I

L

L

V

V

N

V

I

L

N

N

P

N

A

T

Y

Y

R

T

V

P

G

S

E

E

L

L

E

Y

Y

Y

V

A

L

L

R

G

Q

H

S

T

G

D

C

C

R

R

W

L

L

L

V

F

C

L

A

T

E

P

A

R

F

I

K

G

T

R

S

H

D

S

Y

L

H

E

T

E

M

V

V

L

Q

A

E

K

L

L

V

G

P

P

E

R

L

P

A

K

S

E

A

Q

A

G

P

T

G

S

E

S

L

A

A

L

S

E

E

E

C

I

L

I

P

I

E

-

L

L

R

R

G

G

V

Q

I

Y

S

S

L

-

A

-

N

-

P

-

A

-

G

G

F

F

L

S

P

T

W

Y

H

E

A

H

F

V

A

I

E

Q

R

R

A

G

G

L

Q

P

T

L

S

P

V

S

E

H

A

A

C

L

T

Q

A

D

R

R

Q

E

Q

A

S

E

L

L

Q

H

F

S

D

T

Q

D

P

V

V

C

N

N

I

L

Q

Q

Y

F

T

T

S

S

G

G

T

S

T

T

G

G

A

N

P

P

K

K

G

A

A

A

T

M

L

L

S

T

H

H

Y

H

N

N

I

L

L

V

N

N

G

S

F

R

M

F

V

I

G

G

E

D

S

R

L

M

G

N

L

L

T

T

A

S

R

F

D

D

R

I

M

L

V

C

I

C

P

P

V

P

P

P

L

L

Y

F

H

H

C

C

F

F

G

G

M

L

V

V

M

L

A

G

N

M

L

L

G

A

C

V

I

V

T

T

H

H

G

G

S

S

T

K

M

I

I

I

Y

F

P

P

N

S

D

E

A

T

F

F

D

D

A

P

E

T

L

A

T

V

L

L

R

H

A

A

V

I

A

S

E

D

E

E

R

K

A

C

T

T

I

A

L

L

Y

H

G

G

V

V

P

P

T

T

M

M

F

F

I

E

A

A

M

I

L

L

D

S

H

L

P

P

S

K

R

P

A

P

H

N

M

F

D

D

L

C

S

S

T

N

L

L

R

R

S

T

G

G

I

I

M

I

A

A

G

G

A

A

T

P

C

V

P

P

I

R

E

P

V

L

M

M

R

K

R

R

V

L

I

L

D

E

Q

E

M

L

H

N

M

M

A

T

E

E

V

Y

Q

T

I

S

A

S

Y

Y

G

G

M

L

T

T

E

E

T

A

S

S

P

P

V

T

S

C

L

F

Q

N

T

A

G

L

P

T

D

T

D

D

D

S

L

I

E

E

L

R

R

R

V

L

T

T

V

V

G

G

R

K

T

V

Q

M

P

P

Q

H

L

A

E

K

N

A

K

K

L

I

V

I

D

D

A

T

D

Q

G

G

C

H

I

I

V

V

P

P

R

I

G

G

E

Q

I

R

G

G

E

E

L

L

C

C

T

I

R

A

G

G

Y

Y

S

Q

V

L

M

T

L

K

G

G

Y

Y

W

W

D

N

N

N

P

P

Q

E

A

K

T

T

A

A

D

E

A

A

I

L

-

I

-

T

-

D

-

S

D

D

P

G

A

V

G

T

W

W

M

L

H

K

S

T

G

G

D

D

L

E

A

A

V

I

M

F

D

D

E

E

Q

E

G

G

Y

Y

V

C

R

S

I

I

V

T

G

G

R

R

N

F

K

K

D

D

M

I

I

I

R

R

G

G

E

E

N

N

I

I

Y

Y

P

P

R

L

E

E

L

I

E

E

F

R

F

L

Y

A

T

A

H

H

P

P

A

A

V

I

A

E

D

V

A

A

Q

S

V

V

I

I

G

G

I

I

P

P

C

D

S

Q

R

K

Y

Y

G

G

E

E

E

V

I

V

V

G

A

A

W

F

I

L

K

A

L

L

H

A

P

A

G

D

H

V

S

S

A

A

-

R

-

P

T

S

V

D

E

E

L

L

Q

R

G

A

W

W

C

T

K

R

A

E

R

T

I

L

A

G

H

R

F

H

K

K

V

A

P

P

R

Q

Y

Y

I

F

R

F

F

V

V

F

D

G

E

E

-

E

-

G

-

V

-

D

-

R

-

T

Y

I

P

P

M

V

T

T

V

G

T

S

G

G

K

K

V

V

Q

R

K

K

F

V

R

D

M

L

R

R

E

K

I

I

S

A

V

A

A

S

Sites not aligning to the query:

6:14 PTS2-type peroxisomal targeting signal

P69451 Long-chain-fatty-acid--CoA ligase; Long-chain acyl-CoA synthetase; Acyl-CoA synthetase; EC 6.2.1.3 from Escherichia coli (strain K12) (see paper)
32% identity, 95% coverage: 18:548/560 of query aligns to 23:551/561 of P69451

query
sites
P69451

Q

Q

T

S

I

L

G

V

Q

D

A

M

F

F

D

E

A

Q

T

S

V

V

A

A

R

R

C

Y

C

A

D

D

S

Q

E

P

A

A

L

F

V

V

S

N

R

-

H

-

Q

M

G

G

L

E

R

V

Y

M

S

T

W

F

R

R

Q

K

L

L

A

E

E

E

Q

R

V

S

E

R

I

A

Y

F

A

A

R

A

A

Y

L

L

I

Q

-

Q

A

G

L

L

G

G

V

L

N

K

T

K

G

G

D

D

R

R

V

V

G

A

I

L

W

M

S

M

P

P

N

N

C

L

A

L

Q

Q

W

Y

C

P

I

V

L

A

Q

L

L

F

A

G

S

I

A

L

K

R

V

A

G

G

A

M

I

I

L

V

V

V

N

N

I

V

N

N

P

P

A

L

Y

Y

R

T

V

P

G

R

E

E

L

L

E

E

Y

H

V

Q

L

L

R

N

Q

D

S

S

G

G

C

A

R

S

W

A

L

I

V

V

C

I

A

V

E

S

A

N

F

F

-

A

-

H

-

T

-

L

-

E

-

K

-

V

-

D

K

K

T

T

S

A

D

V

Y

Q

H

H

T

V

M

I

V

L

Q

T

E

R

L

M

V

G

P

D

E

Q

L

L

A

-

S

S

A

T

A

A

P

K

G

G

E

T

L

V

A

V

S

N

E

F

C

V

L

V

P

K

E

Y

L

I

R

K

G

R

V

L

I

V

S

-

L

-

A

-

N

-

P

-

P

P

A

K

G

Y

F

H

L

L

P

P

W

-

H

D

A

A

F

I

A

S

E

F

R

R

A

S

G

A

Q

L

T

H

S

N

V

G

E

Y

A

R

C

M

T

Q

A

Y

R

V

Q

K

Q

P

S

E

L

L

Q

V

F

P

D

E

Q

D

P

L

V

A

N

F

I

L

Q

Q

Y
|
Y

T
|
T

S

G

G
|
G

T
|
T

T

T

G
|
G

A

V

P

A

K
|
K

G

G

A

A

T

M

L

L

S

T

H

H

Y

R

N

N

I

M

L

L

N

A

N

N

G

L

F

E

M

Q

V

V

G

N

E

A

S

T

L

Y

G

G

L

P

T

L

-

L

-

H

-

P

A

G

R

K

D

E

R

L

M

V

V

V

I

T

P

A

V

L

P

P

L

L

Y

Y

H

H

C

I

F

F

G

A

M

L

V

T

M

I

A

N

N

C

L

L

G

L

C

F

I

I

T

E

H

L

G

G

S

G

T

Q

M

N

I

L

Y

L

P

I

N

T

D

N

A

P

F

R

D

D

A

I

E

P

L

G

T

L

L

V

R

K

A

E

V

L

A

A

E

K

E

Y

R

P

A

F

T

T

I

A

L

I

Y

T

G

G

V

V

P

N

T

T

M

L

F

F

I

N

A

A

M

L

L

L

D

N

H

N

P

K

S

E

R

F

A

Q

H

Q

M

L

D

D

L

F

S

S

T

S

L

L

R

H

S

-

G

-

I

L

M

S

A

A

G

G

A

G

T

G

C

M

P

P

I

V

E

Q

-

Q

-

V

V

V

M

A

R

E

R

R

V

W

I

V

D

K

Q

L

M

T

H

G

M

Q

A

Y

E

L

V

L

Q

E

I

-

A

G

Y

Y

G

G

M

L

T

T

E
|
E

T

C

S

A

P

P

V

L

S

-

L

V

Q

S

T

V

G

N

P

P

D

Y

D

D

D

-

L

I

E

D

L

Y

R

H

V

S

T

G

T

S

V

I

G

G

R

L

T

P

Q

V

P

P

Q

S

L

T

E

E

N

A

K

K

L

L

V

V

D

D

A

D

D

D

G

D

C

N

I

E

V

V

P

P

R

P

G

G

E

Q

I

P

G

G

E

E

L

L

C

C

T

V

R

K

G

G

Y

P

S

Q

V

V

M

M

L

L

G

G

Y

Y

W

W

D

Q

N

R

P

P

Q

D

A

A

T

T

A

-

D

D

A

E

I

I

D

I

P

K

A

N

G

G

W

W

M

L

H

H

S

T

G

G

D

D

L

I

A

A

V

V

M

M

D

D

E

E

Q

E

G

G

Y

F

V

L

R

R

I

I

V

V

G

D

R

R

N

K

K

K

D

D

M

M

I

I

I

L

R

V

G

S

G

G

E

F

N

N

I

V

Y

Y

P

P

R

N

E

E

L

I

E

E

E

D

F

V

Y

M

T

Q

H

H

P

P

A

G

V

V

A

Q

D

E

A

V

Q

A

V

A

I

V

G

G

I

V

P

P

C

S

S

G

R

S

Y

S

G

G

E

E

E

A

I

V

V

K

A

I

W

F

-

V

I

V

K

K

L

K

H

D

P

P

G

-

H

-

S

S

A

L

T

T

V

E

E

E

E

S

L

L

Q

V

G

T

W

F

C

C

K

R

A

R

R

Q

I

L

A

T

H

G

F

Y

K

K

V

V

P

P

R

K

Y

L

I

V

R

E

F

F

V

R

D

D

E

E

Y

L

P

P

M

K

T

S

V

N

T

V

G

G

K

K

V

I

Q

L

K

R

F

R

R

E

M

L

R

R

E

D

Y213 (= Y208) mutation to A: Loss of activity.
T214 (= T209) mutation to A: 10% of wild-type activity.
G216 (= G211) mutation to A: Decreases activity.
T217 (= T212) mutation to A: Decreases activity.
G219 (= G214) mutation to A: Decreases activity.
K222 (= K217) mutation to A: Decreases activity.
E361 (= E354) mutation to A: Loss of activity.

4gxqA Crystal structure of atp bound rpmatb-bxbclm chimera b1 (see paper)
30% identity, 90% coverage: 43:548/560 of query aligns to 26:499/506 of 4gxqA

query
sites
4gxqA

G

G

L

D

R

K

Y

I

S

S

W

Y

R

A

Q

E

L

L

A

V

E

A

Q

R

V

A

E

G

I

R

Y

V

A

A

R

N

A

V

L

L

I

V

A

A

L

R

G

G

V

L

N

Q

T

V

G

G

D

D

R

R

V

V

G

A

I

A

W

Q

S

T

P

E

N

K

C

S

A

V

Q

E

W

A

C

L

I

V

L

L

Q

Y

L

L

A

A

S

T

A

V

K

R

V

A

G

G

A

G

I

V

L

Y

V

L

N

P

I

L

N

N

P

T

A

A

Y

Y

R

T

V

L

G

H

E

E

L

L

E

D

Y

Y

V

F

L

I

R

T

Q

D

S

A

G

E

C

P

R

K

W

I

L

V

V

V

C

C

A

D

E

P

A

S

F

K

K

R

T

D

S

G

D

-

Y

-

H

-

T

-

M

-

V

-

Q

-

E

-

L

-

V

I

P

A

E

A

L

I

A

A

S

-

A

A

A

K

P

V

G

G

E

A

L

T

A

V

S

E

E

T

C

L

L

G

P

P

E

D

L

G

R

R

G

G

V

S

I

L

S

T

L

-

A

-

N

-

P

-

A

-

G

-

F

-

L

-

P

-

W

-

H

-

A

-

F

-

A

-

E

D

R

A

A

A

G

A

Q

G

T

A

S

S

V

E

E

A

A

F

C

A

T

T

A

I

R

D

Q

R

Q

G

S

A

L

-

Q

-

F

-

D

D

Q

D

P

L

V

A

N

A

I

I

Q

L

Y

Y

T
|
T

S
|
S

G
|
G

T
|
T

G

G

A

R

P

S

K

K

G

G

A

A

T

M

L

L

S

S

H

H

Y

D

N

N

I

L

L

A

N

S

N
|
N

G

S

F

L

M

T

V

L

G

V

E

D

S

Y

L

W

G

R

L

F

T

T

A

P

R

D

D

D

R

V

M

L

V

I

I

H

P

A

V

L

P

P

L

I

Y

Y

H
|
H

C

T

F

H

G

G

M

L

V

F

M

V

A

A

-

S

N

N

L

V

G

T

C

L

I

F

T

A

H

R

G

G

S

S

T

M

M

I

I

F

Y

L

P

P

N

K

D

-

A

-

F

F

D

D

A

P

E

D

L

K

T

I

L

L

R

D

A

L

V

M

A

A

E

-

R

R

A

A

T

T

I

V

L

L

Y

M

G

G

V

V

P

P

T

T

M

F

F

Y

I

T

A

R

M

L

L

L

D

Q

H

S

P

P

S

R

R

L

A

T

H

K

M

E

D

T

L

T

S

G

T

H

L

M

R

R

S

L

G

F

I

I

M

S

A

G

G
x
S

A
|
A

T
x
P

C

L

P

L

I

A

E

D

V

T

M

H

R

R

R

E

V

W

I

S

D

A

Q

K

M

T

H

G

M

H

A

A

E

V

V

L

Q
x
E

I

-

A
x
R

Y

Y

G
|
G

M
|
M

T
|
T

E
|
E

T

T

S

N

P

-

V

-

S

-

L

M

Q

N

T

T

G

S

P

N

D

P

D

Y

D

D

L

G

E

D

L

R

R

V

V

P

T

G

T

A

V

V

G

G

R

P

T

A

Q

L

P

P

Q

G

L

V

E

S

N

A

K

R

L

V

V

T

D

D

A

P

D

E

-

T

G

G

C

K

I

E

V

L

P

P

R

R

G

G

E

D

I

I

G

G

E

M

L

I

C

E

T

V

R

K

G

G

Y

P

S

N

V

V

M

F

L

K

G

G

Y

Y

W

W

D

R

N

M

P

P

Q

E

A

K

T

T

A

K

D

S

A

E

I

F

D

R

P

D

A

D

G

G

W

F

M

F

H

I

S

T

G

G

D
|
D

L

L

A

G

V

K

M

I

D

D

E

E

Q

R

G

G

Y

Y

V

V

R

H

I

I

V

L

G

G

R
|
R

N

G

K

K

D

D

M

L

I

V

I
|
I

R

T

G

G

E

F

N
|
N

I

V

Y

Y

P

P

R

K

E

E

L

I

E

E

E

S

F

E

F

I

Y

D

T

A

H

M

P

P

A

G

V

V

A

V

D

E

A

S

Q

A

V

V

I

I

G

G

I

V

P

P

C

H

S

A

R

D

Y

F

G

G

E

E

E

G

I

V

V

T

A

A

W

F

I

V

K

V

L

L

H

K

P

R

G

E

H

F

S

A

A

P

T

S

-

E

-

I

-

L

V

A

E

E

L

L

Q

K

G

A

W

F

C

V

K

K

A

D

R

R

I

L

A

A

H

K

F

F

K

K

V

M

P

P

R

K

Y

K

I

V

R

I

F

F

V

V

D

D

E

D

Y

L

P

P

M

R

T

N

V

T

T

M

G

G

K
x
A

V

V

Q

Q

K

K

F

N

R

V

M

L

R

R

E

E

active site: T163 (= T209), N183 (= N229), H207 (= H253), T303 (= T353), E304 (= E354), I403 (= I455), N408 (= N460), A491 (≠ K540)
binding adenosine-5'-triphosphate: T163 (= T209), S164 (= S210), G165 (= G211), T166 (= T212), T167 (= T213), H207 (= H253), S277 (≠ G326), A278 (= A327), P279 (≠ T328), E298 (≠ Q347), M302 (= M352), T303 (= T353), D382 (= D434), R397 (= R449)
binding carbonate ion: H207 (= H253), S277 (≠ G326), R299 (≠ A349), G301 (= G351)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
29% identity, 92% coverage: 35:548/560 of query aligns to 19:495/503 of P9WQ37

query
sites
P9WQ37

E

Q

A

A

L

Y

V

V

S

E

R

P

H

S

Q

T

G

D

L

V

R

R

Y

M

S

T

W

Y

R

A

Q

Q

L

M

A

N

E

A

Q

L

V

A

E

N

I

R

Y

C

A

A

R

D

A

V

L

L

I

T

A

A

L

L

G

G

V

I

N

A

T

K

G

G

D

D

R

R

V

V

G

A

I

L

W

L

S

M

P

P

N

N

C

S

A

V

Q

E

W

F

C

C

I

C

L

L

Q

F

L

Y

A

G

S

A

A

A

K

K

V

L

G

G

A

A

I

V

L

A

V

V

N

P

I

I

N

N

P

T

A

R

Y

L

R

A

V

A

G

P

E

E

L

V

E

S

Y

F

V

I

L

L

R

S

Q

D

S

S

G

G

C

S

R

K

W

V

L

V

V

I

C

Y

A

G

E

-

A

-

F

-

K

-

T

-

S

-

D

-

Y

-

H

-

T

-

M

-

V

-

Q

-

E

-

L

-

V

-

P

-

E

-

L

-

A

A

S

P

A

S

A

A

P

P

G

-

E

-

L

-

A

-

S

-

E

-

C

-

L

-

P

-

E

-

L

V

R

I

G

D

V

A

I

I

S

R

L

A

A

Q

A

A

N

D

P

P

A

G

G

T

F

V

L

T

P

D

W

W

-

I

-

G

-

A

H

D

A

S

F

L

A

A

E

E

R

R

A

-

G

-

Q

-

T

-

S

-

V

L

E

R

A

S

C

A

T

A

A

A

R

D

Q

E

Q

P

S

A

L

V

Q

E

F

C

-

G

D

D

Q

D

P

N

V

L

N

F

I

I

Q

M

Y

Y

T

T

S

S

G

G

T

T

G

G

A

H

P

P

K
|
K

G

G

A

V

T

V

L

H

S

T

H

H

Y

E

N

S

I

V

L

H

N

S

N

A

G

A

F

S

M

S

V

W

G

A

E

S

S

T

L

I

G

D

L

V

T
x
R

A

Y

R
|
R

D

D

R

R

M

L

V

L

I

L

P

P

V

L

P

P

L

M

Y

F

H

H

C
x
V

F

A

G
x
A

M

L

V

T

M
x
T

A

V

N

I

L

F

G

S

C

A

I

M

T

R

H

G

G

V

S

T

T

L

M

I

I

S

Y

M

P

P

N

Q

D

-

A

-

F

F

D

D

A

A

E

T

L

K

T

V

L

W

R

S

A

L

V

I

A

V

E

E

R
|
R

A

V

T

C

I

I

L

G

Y

G

G

A

V

V

P

P

T

A

M

I

F

L

I

N

A

F

M

M

L

R

D

Q

H

V

P

P

S

E

R

F

A

A

H

E

M

L

D

D

L

A

S

P

T

D

L

F

R

R

S

Y

G

F

I

I

M

T

A

G

G

G

A

A

T

P

C

M

P

P

I

-

E

E

V

A

M

L

R

I

R

K

V

I

I

Y

D

A

Q

A

M

K

H

N

M

I

A

E

E

V

V

V

Q

Q

I

-

A

G

Y

Y

G
x
A

M

L

T

T

E

E

T

S

S

C

P

G

V

G

S

G

L

T

Q

L

T

L

G

L

P

S

D

E

D

D

D

A

L

L

E

R

L

-

R

K

V

A

T

G

T

S

V

A

G

G

R

R

T

A

Q

T

P

M

Q

F

L

T

E

D

N

V

K

A

L

V

V

R

D

G

A

D

D

D

G

G

C

V

I

I

V

R

P

E

R

H

G

G

E

E

I

-

G

G

E

E

L

V

C

V

T

I

R

K

G

S

Y

D

S

I

V

L

M

L

L

K

G

E

Y

Y

W

W

D

N

N

R

P

P

Q

E

A

A

T

T

A

R

D

D

A

A

I

F

D

D

P

-

A

N

G

G

W
|
W

M

F

H

R

S

T

G

G

D
|
D

L

I

A

G

V

E

M

I

D

D

E

D

Q

E

G

G

Y

Y

V

L

R

Y

I

I

V

K

G

D

R
|
R

N

L

K

K

D

D

M

M

I

I

R
x
S

G

G

G
|
G

E

E

N

N

I

V

Y

Y

P

P

R

A

E

E

L

I

E

E

E

S

F

V

F

I

Y

I

T

G

H

V

P

P

A

G

V

V

A

S

D

E

A

V

Q

A

V

V

I

I

G

G

I

L

P

P

C

D

S

E

R

K

Y

W

G

G

E

-

E

E

I

I

V

A

A

A

W

A

I

I

K

V

L

V

H

A

P

D

G

Q

H

N

S

E

A

V

T

S

V

E

E

Q

L

I

Q

V

G

E

W

Y

C

C

K

G

A

T

R

R

I

L

A

A

H

R

F

Y

K

K

V

L

P

P

R

K

Y

K

I

V

R

I

F

F

V

A

D

E

E

A

Y

I

P

P

M

R

T

N

V

P

T

T

G

G

K
|
K

V

I

Q

L

K

K

F

T

R

V

M

L

R

R

E

E

K172 (= K217) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ T240) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (= R242) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C254) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G256) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ M259) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (= R291) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G351) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (= W429) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D434) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R449) mutation to A: Reduction of binding affinity for fatty acids.
S404 (≠ R456) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G458) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K540) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
28% identity, 92% coverage: 35:548/560 of query aligns to 22:495/502 of 3r44A

query
sites
3r44A

E

Q

A

A

L

Y

V

V

S

E

R

P

H

S

Q

T

G

D

L

V

R

R

Y

M

S

T

W

Y

R

A

Q

Q

L

M

A

N

E

A

Q

L

V

A

E

N

I

R

Y

C

A

A

R

D

A

V

L

L

I

T

A

A

L

L

G

G

V

I

N

A

T

K

G

G

D

D

R

R

V

V

G

A

I

L

W

L

S

M

P

P

N

N

C

S

A

V

Q

E

W

F

C

C

I

C

L

L

Q

F

L

Y

A

G

S

A

A

A

K

K

V

L

G

G

A

A

I

V

L

A

V

V

N

P

I

I

N

N

P

T

A

R

Y

L

R

A

V

A

G

P

E

E

L

V

E

S

Y

F

V

I

L

L

R

S

Q

D

S

S

G

G

C

S

R

K

W

V

L

V

V

I

C

Y

A

G

E

-

A

-

F

-

K

-

T

-

S

-

D

-

Y

-

H

-

T

-

M

-

V

-

Q

-

E

-

L

-

V

-

P

-

E

-

L

-

A

A

S

P

A

S

A

A

P

P

G

-

E

-

L

-

A

-

S

-

E

-

C

-

L

-

P

-

E

-

L

V

R

I

G

D

V

A

I

I

S

R

L

A

A

Q

A

A

N

D

P

P

A

G

G

T

F

V

L

T

P

D

W

W

-

I

-

G

-

A

H

D

A

S

F

L

A

A

E

E

R

R

A

-

G

-

Q

-

T

-

S

-

V

L

E

R

A

S

C

A

T

A

A

A

R

D

Q

E

Q

P

S

A

L

V

Q

E

F

C

-

G

D

D

Q

D

P

N

V

L

N

F

I

I

Q

M

Y

Y

T
|
T

S

S

G

G

T

H

T

-

G

-

A

-

P

P

K

K

G

G

A

V

T

V

L

H

S

T

H

H

Y
x
E

N

S

I

V

L
x
H

N

S

N
x
A

G

A

F

S

M

S

V

W

G

A

E

S

S

T

L

I

G

D

L

V

T

R

A

Y

R

R

D

D

R

R

M

L

V

L

I

L

P

P

V

L

P

P

L

M

Y

F

H
|
H

C

V

F

A

G

A

M

L

V

T

M

T

A

V

N

I

L

F

G

S

C

A

I

M

T

R

H

G

G

V

S

T

T

L

M

I

I

S

Y

M

P

P

N

Q

D

-

A

-

F

F

D

D

A

A

E

T

L

K

T

V

L

W

R

S

A

L

V

I

A

V

E

E

R

R

A

V

T

C

I

I

L

G

Y

G

G

A

V

V

P

P

T

A

M

I

F

L

I

N

A

F

M

M

L

R

D

Q

H

V

P

P

S

E

R

F

A

A

H

E

M

L

D

D

L

A

S

P

T

D

L

F

R

R

S

Y

G

F

I

I

M

T

A

G

G

G

A

A

T

P

C

M

P

P

I

-

E

E

V

A

M

L

R

I

R

K

V

I

I

Y

D

A

Q

A

M

K

H

N

M

I

A

E

E

V

V

V

Q

Q

I

-

A

G

Y

Y

G

A

M

L

T
|
T

E
|
E

T

S

S

C

P

G

V

G

S

G

L

T

Q

L

T

L

G

L

P

S

D

E

D

D

D

A

L

L

E

R

L

-

R

K

V

A

T

G

T

S

V

A

G

G

R

R

T

A

Q

T

P

M

Q

F

L

T

E

D

N

V

K

A

L

V

V

R

D

G

A

D

D

D

G

G

C

V

I

I

V

R

P

E

R

H

G

G

E

E

I

-

G

G

E

E

L

V

C

V

T

I

R

K

G

S

Y

D

S

I

V

L

M

L

L

K

G

E

Y

Y

W

W

D

N

N

R

P

P

Q

E

A

A

T

T

A

R

D

D

A

A

I

F

D

D

P

-

A

N

G

G

W

W

M

F

H

R

S

T

G

G

D

D

L

I

A

G

V

E

M

I

D

D

E

D

Q

E

G

G

Y

Y

V

L

R

Y

I

I

V

K

G

D

R

R

N

L

K

K

D

D

M

M

I

I

I
|
I

R

S

G

G

E

E

N
|
N

I

V

Y

Y

P

P

R

A

E

E

L

I

E

E

E

S

F

V

F

I

Y

I

T

G

H

V

P

P

A

G

V

V

A

S

D

E

A

V

Q

A

V

V

I

I

G

G

I

L

P

P

C

D

S

E

R

K

Y

W

G

G

E

-

E

E

I

I

V

A

A

A

W

A

I

I

K

V

L

V

H

A

P

D

G

Q

H

N

S

E

A

V

T

S

V

E

E

Q

L

I

Q

V

G

E

W

Y

C

C

K

G

A

T

R

R

I

L

A

A

H

R

F

Y

K

K

V

L

P

P

R

K

Y

K

I

V

R

I

F

F

V

A

D

E

E

A

Y

I

P

P

M

R

T

N

V

P

T

T

G

G

K
|
K

V

I

Q

L

K

K

F

T

R

V

M

L

R

R

E

E

active site: T167 (= T209), A184 (≠ N229), H208 (= H253), T304 (= T353), E305 (= E354), I403 (= I455), N408 (= N460), K487 (= K540)
binding histidine: E179 (≠ Y224), H182 (≠ L227)

Sites not aligning to the query:

binding histidine: 5

4wv3B Crystal structure of the anthranilate coa ligase auaeii in complex with anthranoyl-amp (see paper)
30% identity, 91% coverage: 47:557/560 of query aligns to 37:517/518 of 4wv3B

query
sites
4wv3B

S

T

W

F

R

R

Q

Q

L

V

A

S

E

N

Q

E

V

A

E

N

I

Q

Y

V

A

G

R

N

A

A

L

L

I

K

A

G

L

L

G

G

V

V

N

R

T

F

G

G

D

E

R

C

V

V

G

G

I

L

W

L

S

T

P

L

N

D

C

S

A

A

Q

E

W

W

C

V

I

T

L

S

Q

F

L

F

A

G

S

I

A

V

K

K

V

L

G

G

A

A

I

I

L

A

V

V

N

G

I

I

N

N

P

T

A

L

Y

L

R

K

V

P

G

P

E

E

L

Y

E

E

Y

Y

V

I

L

L

R

R

Q

D

S

C

G

R

C

A

R

R

W

V

L

L

V

I

C

V

A

H

E

-

A

-

F

-

K

-

T

-

S

-

D

-

Y

-

H

-

T

-

M

-

V

-

Q

Q

E

E

L

F

V

L

P

P

E

L

L

I

A

E

S

S

A

I

A

R

P

G

G

N

E

-

L

-

A

-

S

-

E

-

C

-

L

L

P

P

E

M

L

L

R

E

G

H

V

I

I

V

S

V

L

I

A

G

A

E

N

G

P

P

P

Q

A

E

G

G

F

Y

L

L

P

S

W

F

H

N

A

D

F

W

A

I

E

-

R

R

A

P

G

Q

Q

P

T

T

S

T

V

L

E

E

A

A

C

A

T

Q

A

S

R

H

Q

R

Q

E

S

D

L

I

Q

-

F

-

D

-

Q

-

P

-

V

C

N

S

I

L

Q

N

Y

Y

T
x
S

S

S

G

G

T

T

G

G

A

G

P

P

K

K

G

G

A

I

T

P

L

H

S

A

H

H

Y

K

N

D

I

Y

L

P

N

L

N

T

G

A

F

Q

M

L

V

W

G

G

E

V

S

N

-

V

L

L

G

G

L

L

T

R

A

E

R

S

D

D

R

R

M

T

V

F

I

A

P

L

V

A

P

K

L

L

Y

F

H
x
F

C
x
T

F
|
F

G

G

M

-

V

T

M

G

A

G

N

N

L

L

-

I

-

F

G

P

C

W

I

Y

T

V

H

G

G

A

S

S

T

C

M

V

I

L

Y

F

P

P

N

G

D

A

A

A

F

R

D

V

A

A

E

S

L

N

T

V

L

L

R

S

A

T

V

I

A

S

E

R

E

F

R

K

A

P

T

T

I

I

L

F

Y

Y

G

N

V

A

P

P

T

T

M

G

F

Y

I

A

A

A

M

A

L

L

D

A

H

L

P

K

S

D

R

F

A

S

H

Q

M

H

D

D

L

L

S

S

T

S

L

L

R

R

S

L

G

C

I

V

M

S

A
|
A

G
x
S

A
x
E

T
x
A

C

L

P

P

I

-

E

A

V

A

M

L

R

W

R

Y

V

A

I

W

D

K

Q

E

M

A

H

T

M

G

A

V

E

D

V

I

Q

I

I

D

A
x
G

Y
x
I

G
|
G

M
x
C

T
|
T

E
|
E

T

N

S

F

P

H

V

I

S

F

L

I

Q

S

T

N

G

R

P

P

D

G

D

D

D

-

L

-

E

-

L

I

R

R

V

P

T

G

T

S

V

S

G

G

R

K

T

P

Q

V

P

E

Q

G

L

Y

E

E

N

L

K

K

L

L

V

V

D

D

A

D

D

E

G

G

C

K

I

T

V

V

P

P

R

A

G

G

E

E

I

I

G

G

E

N

L

V

C

L

T

L

R

R

G

S

Y

E

S

T

V

A

M

A

L

L

G

S

Y

Y

W

W

D

H

N

N

P

F

Q

E

A

K

T

S

A

R

D

Q

A

T

I

F

D

Q

P

-

A

G

G

E

W

W

M

L

H

A

S

T

G

G

D
|
D

L

K

A

Y

V

F

M

V

D

D

E

A

Q

D

G

G

Y

Y

V

Y

R

W

I
x
H

V

A

G

G

R
|
R

N

S

K

D

D

D

M

M

I

L

I
x
K

R

V

G

G

E

I

N
x
W

I

V

Y

S

P

P

R

V

E

E

L

V

E

E

E

S

F

T

F

L

Y

I

T

Q

H

H

P

P

A

A

V

V

A

Q

D

E

A

C

Q

A

V

V

I

I

G

G

I

C

P

P

C

-

S

-

R

-

Y

-

G

-

E

-

E

D

I

L

V

I

-

K

-

P

-

K

A

A

W

F

I

I

K

I

L

L

H

K

P

P

G

Q

-

I

-

P

H

S

S

E

A

A

T

L

V

I

E

R

E

Q

L

I

Q

T

G

D

W

H

C

C

K

T

A

E

R

K

I

M

A

A

H

A

F

Y

K

K

V

R

P

P

R

R

Y

W

I

I

R

E

F

F

V

V

D

T

E

E

Y

L

P

P

M

K

T

T

V

A

T

T

G

G

K
|
K

V

I

Q

Q

K

R

F

F

R

K

M

L

R

R

E

-

I

-

S

S

V

A

A

A

E

K

I

L

A

A

active site: S175 (≠ T209), T320 (= T353), E321 (= E354), K418 (≠ I455), W423 (≠ N460), K502 (= K540)
binding 5'-O-[(S)-[(2-aminobenzoyl)oxy](hydroxy)phosphoryl]adenosine: F220 (≠ H253), T221 (≠ C254), F222 (= F255), A293 (= A325), S294 (≠ G326), E295 (≠ A327), A296 (≠ T328), G316 (≠ A349), I317 (≠ Y350), G318 (= G351), C319 (≠ M352), T320 (= T353), D397 (= D434), H409 (≠ I446), R412 (= R449), K502 (= K540)

Q84P21 Peroxisomal OPC-8:0-CoA ligase 1; 4-coumarate--CoA ligase isoform 9; At4CL9; 4-coumarate--CoA ligase-like 5; EC 6.2.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 93% coverage: 36:553/560 of query aligns to 48:543/546 of Q84P21

query
sites
Q84P21

A

A

L

F

V

I

S

D

R

A

H

S

Q

T

G

G

L

Q

R

N

Y

L

S

T

W

F

R

T

Q

E

L

L

A

W

E

R

Q

A

V

V

E

E

I

S

Y

V

A

A

R

D

A

C

L

L

I

S

A

E

L

I

G

G

V

I

N

R

T

K

G

G

D

H

R

V

V

V

G

L

I

L

W

L

S

S

P

P

N

N

C

S

A

I

Q

L

W

F

C

P

I

V

L

V

Q

C

L

L

A

S

S

V

A

M

K

S

V

L

G

G

A

A

I

I

L

I

V

T

N

T

I

T

N

N

P

P

A

L

Y

N

R

T

V

S

G

N

E

E

L

I

E

A

Y

K

V

Q

L

I

R

K

Q

D

S

S

G

N

C

P

R

V

W

L

L

-

V

-

C

-

A

-

E

-

A

A

F

F

K

T

T

T

S

S

D

-

Y

-

H

-

T

-

M

-

V

-

Q

-

E

Q

L

L

V

L

P

P

E

K

L

I

A

S

S

A

A

A

-

K

-

L

P

P

G

I

E

V

L

L

A

M

S

D

E

E

-

E

-

R

-

V

-

D

C

S

L

V

P

G

E

D

L

V

R

R

G

R

V

L

I

V

S

E

L

M

A

M

A

K

N

K

P

E

P

P

A

S

G

G

F

-

L

-

P

-

W

-

H

N

A

R

F

V

A

K

E

E

R

R

A

V

G

D

Q

Q

T

-

S

-

V

-

E

-

A

-

C

-

T

-

A

-

R

-

Q

-

S

-

L

-

Q

-

F

-

D

D

Q

D

P

T

V

A

N

T

I

L

Q

L

Y

Y

T

S

S

S

G

G

T

T

G

G

A

M

P

S

K

K

G

G

A

V

T

I

L

S

S

S

H

H

Y

R

N

N

I

L

L

I

N

A

N

M

G

V

F

Q

M

T

V

I

G

V

E

N

S

R

L

F

G

G

L

S

T

D

-

D

A

G

R

E

D

Q

R

R

M

F

V

I

I

C

P

T

V

V

P

P

L

M

Y

F

H

H

C

I

F

Y

G

G

M

L

V

A

M

A

A

F

N

A

L

T

G

G

C

L

I

L

T

A

H

Y

G

G

S

S

T

T

M

I

I

I

Y

V

P

L

N

S

D

K

A

-

F

F

D

E

A

M

E

H

L

E

T

M

L

M

R

S

A

A

V

I

A

G

E

K

E

Y

R

Q

A

A

T

T

I

S

L

L

Y

P

G

L

V

V

P

P

T

P

M

I

F

L

I

V

A

A

M

M

L

V

D

N

H

G

P

A

S

D

-

Q

-

I

R

K

A

A

H

K

M

Y

D

D

L

L

S

S

T

S

L

M

R

H

S

T

G

V

I

L

M

C

A

G

G

G

A

A

T

P

C

L

P

S

I

K

E

E

V

V

M

T

R

E

R

G

V

F

I

A

D

E

Q

K

M

Y

H

P

M

T

A

V

E

K

V

I

Q

L

I

Q

A

G

Y

Y

G

G

M

L

T

T

E

E

T

S

S

T

P

G

V

I

S

G

L

A

Q

S

T

T

G

D

P

T

D

V

D

E

L

-

E

S

L

R

R

R

V

Y

T

G

T

T

V

A

G

G

R

K

T

L

Q

S

P

A

Q

S

L

M

E

E

N

G

K

R

L

I

V

V

D

D

-

P

A

V

D

T

G

G

C

Q

I

I

V

L

P

G

R

P

G

K

E

Q

I

T

G

G

E

E

L

L

C

W

T

L

R

K

G

G

Y

P

S

S

V

I

M

M

L

K

G

G

Y

Y

W

F

D

S

N

N

P

E

Q

E

A

A

T

T

A

S

D

S

A

T

I

L

D

D

P

S

A

E

G

G

W

W

M

L

H

R

S

T

G

G

D

D

L

L

A

C

V

Y

M

I

D

D

E

E

Q

D

G

G

Y

F

V

I

R

F

I

V

V

V

G

D

R

R

N

L

K

K

D

E

M

L

I

I

I

K

R

Y

G

K

G

G

E

Y

N

Q

I

V

Y

A

P

P

R

A

E

E

L

L

E

E

E

A

F

L

Y

L

T

T

H

H

P

P

A

E

V

I

A

T

D

D

A

A

Q

A

V

V

I

I

G

P

I

F

P

P

C

D

S

K

R

E

Y

V

G

G

E

Q

E

F

I

P

V

M

A

A

W

Y

I

V

K

V

L

R

H

K

P

T

G

G

H

S

S

S

A

L

T

S

V

E

E

K

E

T

L

I

Q

M

G

E

W

F

C

V

K

A

A

K

R

Q

I

V

A

A

H

P

F

Y

K

K

V

R

P

I

R

R

Y

K

I

V

R

A

F

F

V

V

D

S

E

S

Y

I

P

P

M

K

T

N

V

P

T

S

G

G

K
|
K

V

I

Q

L

K

R

F

K

R

D

M

L

R

I

E

K

I

I

S

A

V

T

A

S

E

N

K530 (= K540) mutation to N: Lossed enzymatic activity.

6qjzA Identificationand characterization of an oxalylfrom grass pea (lathyrus sativuscoa-synthetase l.) (see paper)
30% identity, 91% coverage: 27:534/560 of query aligns to 12:493/504 of 6qjzA

query
sites
6qjzA

T

S

V

V

A

A

R

K

C

T

C

F

D

P

S

S

E

R

A

R

L

G

V

I

S

S

R

L

H

A

Q

G

G

K

L

F

R

D

Y

L

S

T

W

H

R

S

Q

H

L

L

A

N

E

E

Q

L

V

V

E

E

I

S

Y

A

A

A

R

D

A

H

L

L

I

I

A

S

L

A

G

G

V

I

N

K

T

P

G

N

D

D

R

V

V

V

G

A

I

L

W

T

S

F

P

P

N

N

C

T

A

V

Q

E

W

Y

C

V

I

I

L

L

Q

F

L

L

A

A

S

V

A

I

K

R

V

V

G

R

A

A

I

T

L

A

V

A

N

P

I

L

N

N

P

A

A

A

Y

Y

R

T

V

A

G

E

E

E

L

F

E

E

Y

F

V

Y

L

L

R

S

Q

D

S

S

G

E

C

S

R

K

W

L

L

L

V

-

C

-

A

-

E

-

A

-

F

-

K

-

T

-

S

-

D

-

Y

-

H

-

T

-

M

-

V

-

Q

-

E

-

L

L

V

T

P

P

E

-

L

L

A

E

S

G

A

N

A

K

P

P

G

A

E

Q

L

D

A

A

S

A

E

S

C

K

L

L

P

S

E

I

L

P

R

L

G

G

V

S

I

A

S

S

L

L

A

T

A

K

N

S

P

E

P

-

A

-

G

-

F

-

L

-

P

-

W

-

H

-

A

-

F

-

A

E

E

E

R

T

A

K

G

L

Q

T

T

I

S

S

V

L

E

K

A

H

C

P

T

E

A

S

R

G

Q

S

Q

V

S

A

L

K

Q

L

F

I

D

N

Q

E

P

P

V

S

N

D

I

V

Q

A

-

L

-

F

-

L

Y

H

T
|
T

S

S

G

G

T

T

G

S

A

R

P

P

K

K

G

G

A

V

T

P

L

L

S

T

H

Q

Y

H

N

N

I

L

L

V

N

S

N
x
S

G

V

F

K

M

N

V

I

G

Q

E

S

S

V

L

Y

G

Q

L

L

T

T

A

E

R

S

D

D

R

S

M

T

V

V

I

I

P

V

V

L

P

P

L

L

Y

F

H
|
H

C

V

F

H

G

G

M

L

V

I

M

A

A

G

N

L

L

L

G

S

C

S

I

L

T

G

H

S

G

G

S

A

T

A

M

V

I

V

Y

L

P

P

N

A

D

A

A

G

-

R

F

F

D

S

A

A

E

S

L

T

T

F

L

W

R

K

A

D

V

M

A

I

E

Q

E

Y

R

N

A

A

T

T

I

W

L

Y

Y

T

G

A

V

V

P

P

T

T

M

I

F

H

I

Q

A

I

M

I

L

L

D

D

-

R

-

H

-

L

-

N

H

N

P

P

S

E

R

P

A

A

H

Y

M

P

D

K

L

L

S

R

T

F

L

I

R

R

S

S

G

-

I

-

M

C

A
x
S

G
x
A

A
x
S

T

L

C

A

P

P

I

V

E

I

V

L

M

G

R

R

R

-

V

-

I

L

D

E

Q

E

M

S

H

F

M

G

A

A

E

P

V

V

Q

L

I

E

A

A

Y

Y

G
x
A

M
|
M

T
|
T

E
|
E

T

A

S

S

-

H

-

L

-

M

-

S

-

N

P

P

V

L

S

P

L

-

Q

Q

T

N

G

G

P

P

D

H

D

-

L

-

E

-

L

-

R

K

V

A

T

G

T

S

V

V

G

G

R

K

T

P

Q

V

P

G

Q

Q

L

-

E

E

N

M

K

A

L

I

V

L

D

D

A

E

D

S

G

G

C

R

I

V

V

L

P

E

R

A

G

G

E

V

I

N

G

G

E

E

L

V

C

C

T

I

R

R

G

G

Y

E

S

N

V

V

M

T

L

K

G

G

Y

Y

W

K

D

N

N

N

P

E

Q

A

A

A

T

N

A

T

D

A

A

A

I

F

D

-

P

L

A

F

G

G

W

W

M

F

H

H

S

T

G

G

D
|
D

L

I

A

G

V

Y

M

F

D

D

E

S

Q

D

G

G

Y

Y

V

L

R

H

I
x
L

V

V

G

G

R

R

N

I

K
|
K

D

E

M

L

I

I

I
x
N

R

R

G

G

E

E

N
x
K

I

I

Y

S

P

P

R

I

E

E

L

V

E

D

E

A

F

V

F

L

Y

L

T

S

H

H

P

P

A

D

V

V

A

A

D

Q

A

A

Q

V

V

A

I

F

G

G

I

I

P

P

C

D

S

Q

R

K

Y

Y

G

G

E

E

I

I

V

H

A

C

W

A

I

I

K

I

L

P

H

R

P

E

G

G

H

S

S

N

A

I

T

D

V

A

E

E

L

V

Q

L

G

T

W

F

C

C

K

K

A

K

R

N

I

L

A

A

H

S

F

F

K

K

V

V

P

P

R

K

Y

K

I

V

R

F

F

I

V

T

D

D

E

S

Y

L

P

P

active site: T169 (= T209), S189 (≠ N229), H213 (= H253), T314 (= T353), E315 (= E354), N414 (≠ I455), K419 (≠ N460)
binding adenosine monophosphate: H213 (= H253), S288 (≠ A325), A289 (≠ G326), S290 (≠ A327), A312 (≠ G351), M313 (= M352), T314 (= T353), D393 (= D434), L405 (≠ I446), K410 (= K451), K419 (≠ N460)

6k4dA Ancestral luciferase anclamp in complex with atp and d-luciferin (see paper)
29% identity, 92% coverage: 36:549/560 of query aligns to 39:536/539 of 6k4dA

query
sites
6k4dA

A

A

L

L

V

T

S

D

R

A

H

H

Q

T

G

E

L

E

R

N

Y

I

S

S

W

Y

R

A

Q

E

L

L

A

L

E

E

Q

L

V

T

E

C

I

R

Y

L

A

A

R

E

A

S

L

L

I

K

A

N

L

Y

G

G

V

L

N

K

T

Q

G

N

D

N

R

T

V

I

G

A

I

V

W

C

S

S

P

E

N

N

C

N

A

L

Q

Q

W

F

C

F

I

I

L

P

Q

V

L

I

A

A

S

A

A

L

K

Y

V

I

G

G

A

V

I

A

L

V

V

A

N

P

I

V

N

N

P

D

A

K

Y

Y

R

T

V

E

G

R

E

E

L

L

E

I

Y

N

V

S

L

L

R

N

Q

I

S

S

G

K

C

P

R

T

W

I

L

I

V

F

C

C

A

S

E

K

A

K

F

-

K

-

T

-

S

-

D

-

Y

-

H

-

T

-

M

T

V

L

Q

Q

E

K

L

I

V

L

P

Q

E

-

L

-

A

-

S

-

A

-

P

-

G

-

E

-

L

-

A

V

S

K

E

K

C

K

L

L

P

S

E

Y

L

I

R

K

G

K

V

I

I

I

S

I

L

L

A

D

A

S

N

K

P

E

P

D

-

I

A

G

G

G

F

Y

L

Q

P

C

W

L

H

N

A

N

F

F

A

I

E

S

R

Q

A

H

G

S

Q

D

T

A

S

N

V

F

E

N

A

V

C

S

T

N

A

F

R

K

Q

P

Q

N

S

S

L

F

Q

D

F

R

D

D

Q

E

P

Q

V

V

N

A

-

L

I

I

Q

M

Y

N

T

S

S

S

G

G

T

T

G

G

A

L

P

P

K

K

G

G

A

V

T

M

L

L

S

T

H

H

Y

K

N

N

-

L

-

V

-

R

-

F

-

S

-

H

-

C

-

R

-

D

-

P

I

I

L

F

N

G

N

N

G

Q

F

I

M

I

V

P

G

G

E

T

S

A

L

I

G

-

L

L

T

T

A

V

R

-

D

-

R

-

M

-

V

-

I

-

P

-

V

I

P

P

L

F

Y

H

H
|
H

C

G

F
|
F

G

G

M

M

V

-

M

F

A
x
T

N

T

L

L

G

G

C

Y

I

F

T

T

H

C

G

G

S

F

T

R

M

I

I

V

Y

L

P

M

N

H

D

-

A

R

F

F

D

E

A

E

E

E

L

L

T

F

L

L

R

K

A

S

V

L

A

Q

E

D

E

Y

R

K

A

V

T

Q

I

S

L

T

Y

L

G

L

V

V

P

P

T

T

M

L

F

M

I

A

A

F

M

F

L

A

D

K

H

S

P

P

S

L

R

V

A

D

H

K

M

Y

D

D

L

L

S

S

T

N

L

L

R

K

S

E

G

I

I

A

M

S

A

G

G
|
G

A
|
A

T
x
P

C

L

P

S

I

K

E

E

V

V

M

G

R

E

R

A

V

V

I

A

D

K

Q

R

M

F

H

K

M

L

A

P

E

G

V

I

Q
x
R

I

Q

A
x
G

Y
|
Y

G
|
G

M
x
L

T
|
T

E

E

T

T

S

T

P
x
S

V
x
A

S

I

L

I

Q

I

T

T

G

-

P

P

D

E

D

G

D

D

L

V

E

-

L

-

R

K

V

P

T

G

T

S

V

T

G

G

R

K

T

V

Q

V

P

P

Q

F

L

F

E

S

N

A

K

K

L

V

V

V

D

D

A

L

D

D

-

T

G

G

C

K

I

T

V

L

P

G

R

P

G

N

E

Q

I

R

G

G

E

E

L

L

C

C

T

F

R

K

G

G

Y

D

S

M

V

I

M

M

L

K

G

G

Y

Y

W

V

D

N

N

N

P

P

Q

E

A

A

T

T

A

K

D

E

A

I

I

I

D

D

P

K

A

D