SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_4422 FitnessBrowser__Putida:PP_4422 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
51% identity, 98% coverage: 11:488/490 of query aligns to 2:481/482 of P25526

query
sites
P25526

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WN 156:157 (= WN 163:164) binding NADP(+)
KPAS 180:183 (≠ KPAP 187:190) binding NADP(+)
GS 233:234 (= GS 240:241) binding NADP(+)
L256 (= L263) binding NADP(+)
E386 (= E393) binding NADP(+)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
51% identity, 98% coverage: 11:488/490 of query aligns to 1:480/481 of 3jz4A

query
sites
3jz4A

K

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active site: N156 (= N164), K179 (= K187), E254 (= E262), C288 (= C296), E385 (= E393), E462 (= E470)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P162), W155 (= W163), K179 (= K187), A181 (= A189), S182 (≠ P190), A212 (= A220), G216 (≠ S224), G232 (= G240), S233 (= S241), I236 (≠ V244), C288 (= C296), K338 (= K346), E385 (= E393), F387 (= F395)

8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
47% identity, 98% coverage: 11:490/490 of query aligns to 19:500/505 of 8of1A

query
sites
8of1A

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L

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binding nicotinamide-adenine-dinucleotide: I170 (= I160), A171 (≠ T161), P172 (= P162), W173 (= W163), K197 (= K187), A230 (= A220), F248 (= F238), G250 (= G240), S251 (= S241), V254 (= V244), M257 (≠ I247), L273 (= L263), C306 (= C296), K356 (= K346), E403 (= E393), F405 (= F395)

P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
48% identity, 97% coverage: 17:490/490 of query aligns to 58:535/535 of P51649

query
sites
P51649

L

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C93 (= C52) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
G176 (= G135) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
H180 (≠ P139) to Y: 83% of activity; dbSNP:rs2760118
P182 (≠ H141) to L: 48% of activity; dbSNP:rs3765310
R213 (= R172) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
C223 (= C182) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
KPAE 228:231 (≠ KPAP 187:190) binding NAD(+)
T233 (= T192) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
A237 (= A196) to S: 65% of activity; dbSNP:rs62621664
N255 (≠ Q214) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
G268 (≠ S224) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
GSTTTG 284:289 (≠ GSTEVG 240:245) binding NAD(+)
R334 (≠ A290) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
N335 (≠ T291) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
C340 (= C296) modified: Disulfide link with 342, In inhibited form
C342 (≠ G298) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
N372 (≠ H327) natural variant: N -> S
P382 (= P337) to L: in SSADHD; 2% of activity
V406 (≠ L361) to I: in dbSNP:rs143741652
G409 (= G364) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
S498 (≠ T453) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
G533 (= G488) to R: in SSADHD; <1% of activity; dbSNP:rs72552284

Sites not aligning to the query:

36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832

2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
47% identity, 97% coverage: 17:490/490 of query aligns to 8:485/485 of 2w8rA

query
sites
2w8rA

L

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K

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active site: N155 (= N164), K178 (= K187), E256 (= E262), A290 (≠ C296), E388 (= E393), E465 (= E470)
binding adenosine-5'-diphosphate: T152 (= T161), K178 (= K187), A214 (= A220), S235 (= S241), T238 (≠ V244)

2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
47% identity, 97% coverage: 17:490/490 of query aligns to 8:485/485 of 2w8qA

query
sites
2w8qA

L

L

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S

V

L

L

L

R

R

K

K

W

W

A

Y

A

N

L

L

M

M

R

I

E

Q

N

N

Q

K

E

D

D

D

L

L

A

A

V

R

I

I

M

I

T

T

C

A

E

E

Q

S

G

G

K

K

P

P

L

L

S

K

E

E

S

A

R

H

G

G

E

E

I

I

N

L

Y

Y

G

S

A

A

N

F

F

F

V

L

E

E

W

W

F

F

A

S

A

E

E

E

G

A

E

R

R

R

C

V

Y

Y

G

G

E

D

T

I

I

I

P

H

S

T

H

P

L

A

P

K

N

D

S

R

Q

R

L

A

V

L

T

V

K

L

L

K

Q

Q

P

P

I

I

G

G

V

V

T

A

V

A

A

V

I

I

T

T

P

P

W

W

N
|
N

F

F

P

P

S

S

A

A

M

M

I

I

T

T

R
|
R

K

K

A

V

A

G

A

A

L

L

A

A

G

G

C

C

P

T

M

V

I

V

V

V

K
|
K

P

P

A

A

P

E

E

D

T

T

P

P

L

F

S

S

A

A

L

L

A

A

L

L

A

A

R

E

L

L

A

A

E

S

E

Q

A

A

G

G

I

I

P

P

G

S

G

G

V

V

F

Y

Q

N

V

V

L

I

-

P

-

C

-

S

T

R

G

K

D

N

A

A

P

K

K

E

M

V

S

G

A

E

R

A

L

I

L

C

A

T

A

D

S

P

A

L

V

V

R

S

A

K

F

I

S

S

F

F

T

T

G

G

S

S

T

T

E

T

V

T

G

G

R

K

I

I

L

L

R

H

Q

H

S

A

A

A

D

N

T

S

V

V

K

K

K

R

V

V

S

S

L

M

E
|
E

L

L

G

G

H

L

A

A

P

P

F

F

I

I

V

V

F

F

D

D

D

S

A

A

S

N

I

V

A

D

E

Q

A

A

V

V

E

A

G

G

C

A

I

M

G

A

A

S

K

K

F

F

A
x
R

T

N

S

T

G

G

Q

Q

D

T

C
x
A

L

V

G

C

A

S

N

N

R

Q

I

F

Y

L

V

V

H

Q

R

R

N

G

I

I

Y

H

G

D

Q

A

F

F

V

V

E

K

E

A

F

F

T

A

K

E

A

A

T

M

E

K

K

K

-

N

L

L

R

R

V

V

G

G

H

N

G

G

L

F

E

E

G

G

V

T

D

T

I

Q

G

G

P

P

M

L

T

I

R

N

V

E

T

K

V

A

A

V

N

E

K

K

C

V

R

E

E

K

Q

Q

I

V

S

N

N

D

A

A

L

V

S

S

L

K

G

G

A

A

K

T

L

V

T

V

C

T

G

G

I

K

D

R

N

H

H

Q

L

L

G

G

S

K

S

N

F

F

V

F

L

E

P

P

T

T

V

L

L

L

A

C

D

N

V

V

T

T

D

Q

K

D

M

M

D

L

I

C

A

T

F

H

E

E

E
|
E

T

T

F

F

G

G

P

P

V

L

A

A

A

P

I

V

L

I

P

K

F

F

D

D

S

T

E

E

D

E

E

E

V

A

V

I

T

A

R

I

A

A

N

N

N

A

T

A

E

D

F

V

G

G

L

L

A

A

A

G

Y

Y

V

F

Y

Y

T

S

N

Q

D

D

L

P

R

A

R

Q

A

I

C

W

R

R

V

V

S

A

D

E

R

Q

L

L

E

E

Y

V

G

G

M

M

V

V

A

G

L

V

N

N

T

E

P

G

K

L

F

I

T
x
S

G

S

A

V

P

E

I

C

P

P

F

F

G

G

W

V

K

K

Q

Q

S

S

G

G

L

L

G

G

R

R

E
|
E

G

G

S

S

K

K

H

Y

G

G

L

I

A

D

E

E

Y

Y

M

L

E

E

L

L

K

K

Y

Y

V

V

C

C

I

Y

G

G

L

L

active site: N155 (= N164), K178 (= K187), E256 (= E262), A290 (≠ C296), E388 (= E393), E465 (= E470)
binding succinic acid: R163 (= R172), R284 (≠ A290), A290 (≠ C296), S448 (≠ T453)

8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
46% identity, 98% coverage: 12:490/490 of query aligns to 2:482/482 of 8c54A

query
sites
8c54A

L

L

R

K

R

D

N

P

D

A

L

L

E

T

T

D

R

K

A

A

Y

F

I

V

D

A

G

G

R

A

W

W

V

I

T

G

G

A

E

P

G

D

-

G

-

K

S

S

L

I

D

A

V

V

T

T

D

D

P

P

A

F

N

D

D

G

Q

A

V

L

I

I

A

A

Q

N

V

V

A

P

H

D

C

L

D

G

E

Q

S

A

W

V

V

V

D

A

L

Q

A

A

V

I

E

D

A

A

S

A

R

E

A

A

F

Q

D

K

G

L

W

W

R

E

N

R

T

R

L

T

P

A

T

K

Q

E

R

R

G

A

A

Q

V

V

L

L

R

K

K

R

W

W

A

Y

A

D

L

L

M

I

R

V

E

E

N

N

Q

A

E

D

D

D

L

L

A

A

V

L

I

I

M

L

T

T

C

T

E

E

Q

Q

G

G

K

K

P

P

L

L

S

A

E

E

S

A

R

R

G

G

E

E

I

V

N

V

Y

S

G

N

A

A

N

A

F

Y

V

L

E

E

W

W

F

F

A

A

A

E

E

E

G

A

E

K

R

R

C

I

Y

D

G

G

E

D

T

I

I

I

P

P

S

G

H

P

L

N

P

A

N

G

S

Q

Q

R

L

I

V

M

T

V

K

L

L

K

Q

Q

P

P

I

V

G

G

V

V

T

C

V

A

A

A

I
|
I

T
|
T

P
|
P

W

W

N

N

F

F

P

P

S

N

A

G

M

M

I

I

T

T

R

R

K

K

A

A

A

G

A

P

A

A

L

L

A

A

G

G

C

C

P

S

M

M

I

I

V

L

K
|
K

P

P

A

A

P

S

E

Q

T

T

P

P

L

L

S

S

A

A

L

L

A

A

L

L

A

A

R

V

L

L

A

A

E

E

E

R

A

A

G

G

I

V

P

P

G

K

G

G

V

V

F

F

Q

S

V

V

L

V

T

T

G

G

D

E

A
|
A

P
x
K

K

P

M

I

S

G

A

L

R

E

L

F

L

C

A

H

A

N

S

P

A

R

V

I

R

A

A

K

F

I

S

T

F
|
F

T
|
T

G
|
G

S
|
S

T

T

E

G

V
|
V

G

G

R

R

I
x
W

L

L

L

M

R

K

Q

E

S

A

A

A

D

G

T

G

V

I

K

K

K

R

V

L

S

S

L

L

E

E

L

L

G
|
G

G

G

H

N

A

A

P

P

F

F

I

I

V

V

F

F

D

D

A

A

S

D

I

L

A

D

E

A

A

A

V

V

E

E

G

G

C

A

I

M

G

I

A

S

K

K

F

F

A

R

T

N

S

A

G

G

Q

Q

D

T

C

C

L

V

G

C

A

A

N

N

R

R

I

I

Y

Y

V

V

H

Q

R

E

N

S

I

V

Y

A

G

A

Q

A

F

F

V

T

E

E

E

R

F

L

T

L

K

A

A

K

T

V

E

S

K

G

L

L

R

S

V

L

G

G

H

R

G

G

L

T

E

D

E

A

G

G

V

V

D

S

I

Q

G

G

P

P

M

L

T

I

R

D

V

E

T

K

V

A

A

V

N

A

K

K

C

M

R

E

E

E

Q

H

I

I

S

A

N

D

A

A

L

M

S

A

L

N

G

G

A

G

K

T

L

V

T

L

C

T

G

G

I

K

D

R

N

S

H

V

L

L

G

G

S

G

S

T

F

F

V

F

L

E

P

P

T

T

V

V

L

V

A

T

D

G

V

V

T

T

D

Q

K

T

M

M

D

K

I

V

A

A

F

K

E

E

T

T

F

F

G

A

P

P

V

L

A

A

A

P

I

I

L

I

P

S

F

F

D

K

S

H

E

E

D

N

E

D

V

V

V

I

T

A

R

M

A

A

N

N

N

D

T

S

E

E

F

F

G

G

L

L

A

A

A

S

Y

Y

V

F

Y

Y

T

A

N

K

D

D

L

M

R

A

R

R

A

I

C

W

R

R

V

V

S

A

D

E

R

A

L

L

E

E

Y

A

G

G

M

M

V

V

A

G

L

V

N

N

T

T

P

G

K

M

F

I

T

A

G

N

A

E

P

M

I

A

P

P

F

F

G

G

W

I

K

K

Q

Q

S

S

G

G

L

T

G

G

R

R

E

E

G

G

S

S

K

K

H

Y

G

G

L

I

A

E

E

G

Y

F

M

L

E

E

L

L

K

K

Y

Y

V

V

C

A

I

L

G

G

L

M

binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I160), T153 (= T161), P154 (= P162), K179 (= K187), A212 (= A220), K213 (≠ P221), F230 (= F238), T231 (= T239), G232 (= G240), S233 (= S241), V236 (= V244), W239 (≠ I247), G256 (= G264)

5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
41% identity, 96% coverage: 19:487/490 of query aligns to 4:474/476 of 5x5uA

query
sites
5x5uA

E

D

T

T

R

Q

A

L

Y

L

I

I

D

D

G

G

R

E

W

W

V

V

T
x
D

G
x
A

E
x
A

G

S

-
x
G

-

K

S

T

L

I

D

D

V

V

T

V

D

N

P

P

A

A

N

T

D

G

Q

K

V

P

I

I

A

G

Q

R

V

V

A

A

H

H

C

A

D

G

E

I

S

A

W

D

V

L

D

D

L

R

A

A

V

L

E

A

A

A

S

Q

R

S

A

G

F

F

D

E

G

A

W

W

R

R

N

K

T

V

L

P

P

A

T

H

Q

E

R

R

G

A

A

A

V

T

L

M

R

R

K

K

W

A

A

A

L

L

M

V

R

R

E

E

N

R

Q

A

E

D

D

A

L

I

A

A

V

Q

I

L

M

M

T

T

C

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

S

T

E

E

S

A

R

R

G

V

E

E

I

V

N

L

Y

S

G

A

A

A

N

D

F

I

V

I

E

E

W

W

F

F

A

A

A

D

E

E

G

G

E

R

R

R

C

V

Y

Y

G

G

E

R

T

I

I

V

P

P

S

P

H

R

L

N

P

L

N

G

S

A

Q

Q

L

Q

V

T

T

V

K

V

L

K

Q

E

P

P

I

V

G

G

V

P

T

V

V

A

A

A

I

F

T

T

P
|
P

W

W

N
|
N

F

F

P

P

S

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

A

S

A

A

L

L

A

A

A

T

G

G

C

C

P

S

M

F

I

L

V

V

K
|
K

P

A

A

P

P

E

E

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

R

R

L

A

A

F

E

V

E

D

A

A

G

G

I

V

P

P

G

A

G

G

V

V

F

I

Q

G

V

L

L

V

T

Y

G

G

D

D

A
x
P

P
x
A

K

E

M

I

S
|
S

A

S

R

Y

L

L

L

I

A

P

A

H

S

P

A

V

V

I

R

R

A

K

F

V

S

T

F

F

T

T

G
|
G

S
|
S

T

T

E

P

V
|
V

G

G

R

K

I

Q

L

L

L

A

R

S

Q

L

S

A

A

G

D

L

T

H

V

M

K

K

K

R

V

A

S

T

L

M

E
|
E

L

L

G

G

H

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

S

D

I

V

A

A

E

L

A

A

V

V

E

K

G

A

C

A

I

G

G

G

A

A

K

K

F

F

A

R

T

N

S

A

G

G

Q

Q

D

V

C
|
C

L

I

G

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

R

N

N

S

I

I

Y

R

G

D

Q

E

F

F

V

T

E

R

E

A

F

L

T

V

K

K

A

H

T

A

E

E

K

G

L

L

R

K

V

V

G

G

H

N

G

G

L

L

E

E

G

G

V

T

D

T

I

L

G

G

P

A

M

L

T

A

R

N

V

P

T
x
R

V
x
R

A

L

N

T

K

A

C

M

R

A

E

S

Q

V

I

I

S

D

N

N

A

A

L

R

S

K

L

V

G

G

A

A

K

S

L

I

T

E

C

T

G

G

I

-

D

-

N

E

H

R

L

I

G

G

S

S

-

E

-

G

S

N

F

F

V

F

L

A

P

P

T

T

V

V

L

I

A

A

D

N

V

V

T

P

D

L

K

D

M

A

D

D

I

V

A

F

F

N

E

N

E
|
E

T

P

F
|
F

G

G

P

P

V

V

A

A

I

I

L

R

P

G

F

F

D

D

S

K

E

L

D

E

E

E

V

A

V

I

T

A

R

E

A

A

N

N

N

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

V

A

Y

F

T

T

N

R

D

S

L

F

R

A

R

N

A

V

C

H

R

L

V

L

S

T

D

Q

R

R

L

L

E

E

Y

V

G

G

M

M

V

L

A

W

L

I

N

N

T

Q

P

P

K

A

F

T

T

P

G

W

A

P

P

E

I

M

P

P

F

F

G

G

W

V

K

K

Q

D

S

S

G

G

L

Y

G

G

R

S

E
|
E

G

G

S

G

K

P

H

E

G

A

L

L

A

E

E

P

Y

Y

M

L

E

V

L

T

K

K

Y

S

V

V

C

T

I

V

active site: N151 (= N164), K174 (= K187), E249 (= E262), C283 (= C296), E380 (= E393), E457 (= E470)
binding glycerol: D15 (≠ T30), A16 (≠ G31), A17 (≠ E32), G19 (vs. gap)
binding nicotinamide-adenine-dinucleotide: P149 (= P162), P207 (≠ A220), A208 (≠ P221), S211 (= S224), G227 (= G240), S228 (= S241), V231 (= V244), R329 (≠ T342), R330 (≠ V343), E380 (= E393), F382 (= F395)

5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
41% identity, 96% coverage: 19:487/490 of query aligns to 4:474/476 of 5x5tA

query
sites
5x5tA

E

D

T

T

R

Q

A

L

Y

L

I

I

D

D

G

G

R

E

W

W

V

V

T

D

G

A

E

A

G

S

-

G

-

K

S

T

L

I

D

D

V

V

T

V

D

N

P

P

A

A

N

T

D

G

Q

K

V

P

I

I

A

G

Q

R

V

V

A

A

H

H

C

A

D

G

E

I

S

A

W

D

V

L

D

D

L

R

A

A

V

L

E

A

A

A

S

Q

R

S

A

G

F

F

D

E

G

A

W

W

R

R

N

K

T

V

L

P

P

A

T

H

Q

E

R

R

G

A

A

A

V

T

L

M

R

R

K

K

W

A

A

A

L

L

M

V

R

R

E

E

N

R

Q

A

E

D

D

A

L

I

A

A

V

Q

I

L

M

M

T

T

C

Q

E

E

Q

Q

G

G

K

K

P

P

L

L

S

T

E

E

S

A

R

R

G

V

E

E

I

V

N

L

Y

S

G

A

A

A

N

D

F

I

V

I

E

E

W

W

F

F

A

A

A

D

E

E

G

G

E

R

R

R

C

V

Y

Y

G

G

E

R

T

I

I

V

P

P

S

P

H

R

L

N

P

L

N

G

S

A

Q

Q

L

Q

V

T

T

V

K

V

L

K

Q

E

P

P

I

V

G

G

V

P

T

V

V

A

A

A

I

F

T

T

P

P

W

W

N
|
N

F

F

P

P

S

V

A

N

M

Q

I

V

T

V

R

R

K

K

A

L

A

S

A

A

L

L

A

A

A

T

G

G

C

C

P

S

M

F

I

L

V

V

K
|
K

P

A

A

P

P

E

E

E

T

T

P

P

L

A

S

S

A

P

L

A

A

A

L

L

A

L

R

R

L

A

A

F

E

V

E

D

A

A

G

G

I

V

P

P

G

A

G

G

V

V

F

I

Q

G

V

L

L

V

T

Y

G

G

D

D

A

P

P

A

K

E

M

I

S

S

A

S

R

Y

L

L

L

I

A

P

A

H

S

P

A

V

V

I

R

R

A

K

F

V

S

T

F

F

T

T

G

G

S

S

T

T

E

P

V

V

G

G

R

K

I

Q

L

L

L
x
A

R

S

Q

L

S

A

A

G

D

L

T

H

V

M

K

K

K

R

V

A

S

T

L

M

E
|
E

L

L

G

G

H

H

A

A

P

P

F

V

I

I

V

V

F

A

D

E

D

D

A

A

S

D

I

V

A

A

E

L

A

A

V

V

E

K

G

A

C

A

I

G

G

G

A

A

K

K

F

F

A

R

T

N

S

A

G

G

Q

Q

D

V

C
|
C

L

I

G

S

A

P

N

T

R

R

I

F

Y

L

V

V

H

H

R

N

N

S

I

I

Y

R

G

D

Q

E

F

F

V

T

E

R

E

A

F

L

T

V

K

K

A

H

T

A

E

E

K

G

L

L

R

K

V

V

G

G

H

N

G

G

L

L

E

E

G

G

V

T

D

T

I

L

G

G

P

A

M

L

T

A

R

N

V

P

T

R

V

R

A

L

N

T

K

A

C

M

R

A

E

S

Q

V

I

I

S

D

N

N

A

A

L

R

S

K

L

V

G

G

A

A

K

S

L

I

T

E

C

T

G

G

I

-

D

-

N

E

H

R

L

I

G

G

S

S

-

E

-

G

S

N

F

F

V

F

L

A

P

P

T

T

V

V

L

I

A

A

D

N

V

V

T

P

D

L

K

D

M

A

D

D

I

V

A

F

F

N

E

N

E
|
E

T

P

F

F

G

G

P

P

V

V

A

A

I

I

L

R

P

G

F

F

D

D

S

K

E

L

D

E

E

E

V

A

V

I

T

A

R

E

A

A

N

N

N

R

T

L

E

P

F

F

G

G

L

L

A

A

A

G

Y

Y

V

A

Y

F

T

T

N

R

D

S

L

F

R

A

R

N

A

V

C

H

R

L

V

L

S

T

D

Q

R

R

L

L

E

E

Y

V

G

G

M

M

V

L

A

W

L

I

N

N

T

Q

P

P

K

A

F

T

T

P

G

W

A

P

P

E

I

M

P

P

F

F

G

G

W

V

K

K

Q

D

S

S

G

G

L
x
Y

G

G

R

S

E
|
E

G

G

S

G

K

P

H

E

G

A

L

L

A

E

E

P

Y

Y

M

L

E

V

L

T

K

K

Y

S

V

V

C

T

I

V

active site: N151 (= N164), K174 (= K187), E249 (= E262), C283 (= C296), E380 (= E393), E457 (= E470)
binding glycerol: A236 (≠ L249), Y454 (≠ L467)

5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
37% identity, 96% coverage: 19:487/490 of query aligns to 16:488/491 of 5gtlA

query
sites
5gtlA

E

E

T

I

R

K

A

M

Y

F

I

I

D

N

G

G

R

E

W

F

V

V

T

S

G

A

E

I

G

G

-

G

-

K

S

T

L

F

D

E

V

T

T

Y

D

N

P

P

A

A

N

T

D

E

Q

D

V

V

I

L

A

A

Q

V

V

V

A

C

H

E

C

A

D

Q

E

E

S

E

W

D

V

I

D

D

L

A

A

A

V

V

E

K

A

A

S

R

R

S

A

A

F

F

D

E

G

S

-

G

-

P

W

W

R

A

N

E

T

M

L

T

P

T

T

A

Q

E

R

R

G

A

A

H

V

L

L

I

R

Y

K

K

W

L

A

A

A

D

L

L

M

I

R

E

E

E

N

H

Q

R

E

E

D

E

L

L

A

A

V

Q

I

L

M

E

T

A

C

L

E

D

Q

N

G

G

K

K

P

P

L

Y

S

Q

E

V

S

A

-

L

R

D

G

D

E

D

I

I

N

S

Y

A

G

T

A

V

N

E

F

N

V

Y

E

R

W

Y

F

Y

A

A

A

G

E

W

G

T

E

T

R

K

C

I

Y

I

G

G

E

Q

T

T

I

I

P

P

S

-

H

-

L

I

P

S

N

K

S

D

Q

Y

L

L

-

N

V

Y

T

T

K

R

L

H

Q

E

P

P

I

V

G

G

V

V

T

V

V

G

A

Q

I
|
I

T

I

P
|
P

W

W

N
|
N

F

F

P

P

S

L

A

V

M

M

I

S

T

S

R

W

K

K

A

M

A

G

A

A

L

L

A

A

A

T

G

G

C

C

P

T

M

I

I

V

V

L

K
|
K

P

P

A
|
A

P
x
E

E
x
Q

T

T

P

P

L

L

S

S

A

L

L

L

A

Y

L

A

A

A

R

K

L

L

A

F

E

K

E

E

A

A

G

G

I

F

P

P

G

N

G

G

V

V

F

V

Q

N

V

F

L

V

T

P

G

G

D

F

A
x
G

P

P

K

E

M

A

S
x
G

A

A

R

A

L

I

L

V

A

N

A

H

S

H

A

D

V

I

R

D

A

K

F

V

S

A

F

F

T

T

G
|
G

S
|
S

T

T

E

V

V
x
T

G

G

R

K

I

Y

L

I

L

M

R

R

Q

Q

S

S

A

A

D

E

T

M

V

I

K

K

K

H

V

V

S

T

L

L

E
|
E

L
|
L

G

G

H

K

A

S

P

P

F

N

I

I

V

I

F

L

D

E

D

D

A

A

S

D

I

L

A

E

E

E

A

A

V

I

E

N

G

G

C

A

I

F

G

Q

A

G

K

I

F

M

A

Y

T

N

S

H

G

G

Q

Q

D

N

C
|
C

L

S

G

A

A

G

N

S

R

R

I

V

Y

F

V

V

H

H

R

R

N

K

I

H

Y

Y

G

E

Q

T

F

V

V

V

E

D

E

A

F

L

T

V

K

K

A

M

T

A

E

N

K

N

L

V

R

K

V

L

G

G

H

A

G

G

L

M

E

E

E

K

G

E

V

T

D

E

I

M

G

G

P

P

M

L

T

-

R

-

V

-

T

-

V

V

A

S

N

K

K

K

C

Q

R

Q

E

E

Q

R

I

V

S

L

N

N

A

Y

L

I

S

E

L

Q

G

G

A

K

K

K

-

E

-

G

-

A

-

T

L

V

T

A

C

A

G

G

I

E

D

R

N

A

H

L

L

E

G

K

S

G

S

Y

F

F

V

V

L

K

P

P

T

T

V

V

L

F

A

T

D

D

V

V

T

T

D

D

K

D

M

M

D

T

I

I

A

V

F

K

E

E

E
|
E

T

I

F
|
F

G

G

P

P

V

V

A

V

I

V

L

L

P

P

F

F

D

D

S

S

E

T

D

E

E

E

V

V

V

I

T

E

R

R

A

A

N

N

T

S

E

S

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

W

T

T

N

Q

D

N

L

I

R

K

R

T

A

G

C

H

R

Q

V

V

S

A

D

N

R

K

L

L

E

K

Y

A

G

G

M

T

V

V

A

W

L

I

N

N

T

D

P

Y

K

N

F

L

T

E

G

N

A

A

P

A

I

A

P

P

F

F

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

I

G

G

R

R

E
|
E

G

L

S

G

K

S

H

Y

G

A

L

L

A

D

E

N

Y

Y

M

T

E

E

L

V

K

K

Y

S

V

V

C

W

I

V

active site: N165 (= N164), K188 (= K187), E263 (= E262), C297 (= C296), E394 (= E393), E471 (= E470)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I160), P163 (= P162), K188 (= K187), A190 (= A189), E191 (≠ P190), Q192 (≠ E191), G221 (≠ A220), G225 (≠ S224), G241 (= G240), S242 (= S241), T245 (≠ V244), L264 (= L263), C297 (= C296), E394 (= E393), F396 (= F395)

5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
37% identity, 96% coverage: 19:487/490 of query aligns to 16:488/491 of 5gtkA

query
sites
5gtkA

E

E

T

I

R

K

A

M

Y

F

I

I

D

N

G

G

R

E

W

F

V

V

T

S

G

A

E

I

G

G

-

G

-

K

S

T

L

F

D

E

V

T

T

Y

D

N

P

P

A

A

N

T

D

E

Q

D

V

V

I

L

A

A

Q

V

V

V

A

C

H

E

C

A

D

Q

E

E

S

E

W

D

V

I

D

D

L

A

A

A

V

V

E

K

A

A

S

R

R

S

A

A

F

F

D

E

G

S

-

G

-

P

W

W

R

A

N

E

T

M

L

T

P

T

T

A

Q

E

R

R

G

A

A

H

V

L

L

I

R

Y

K

K

W

L

A

A

A

D

L

L

M

I

R

E

E

E

N

H

Q

R

E

E

D

E

L

L

A

A

V

Q

I

L

M

E

T

A

C

L

E

D

Q

N

G

G

K

K

P

P

L

Y

S

Q

E

V

S

A

-

L

R

D

G

D

E

D

I

I

N

S

Y

A

G

T

A

V

N

E

F

N

V

Y

E

R

W

Y

F

Y

A

A

A

G

E

W

G

T

E

T

R

K

C

I

Y

I

G

G

E

Q

T

T

I

I

P

P

S

-

H

-

L

I

P

S

N

K

S

D

Q

Y

L

L

-

N

V

Y

T

T

K

R

L

H

Q

E

P

P

I

V

G

G

V

V

T

V

V

G

A

Q

I
|
I

T
x
I

P
|
P

W
|
W

N
|
N

F

F

P

P

S

L

A

V

M

M

I

S

T

S

R

W

K

K

A

M

A

G

A

A

L

L

A

A

A

T

G

G

C

C

P

T

M

I

I

V

V

L

K
|
K

P

P

A

A

P
x
E

E

Q

T

T

P

P

L

L

S

S

A

L

L

L

A

Y

L

A

A

A

R

K

L

L

A

F

E

K

E

E

A

A

G

G

I

F

P

P

G

N

G

G

V

V

F

V

Q

N

V

F

L

V

T

P

G

G

D

F

A
x
G

P

P

K

E

M

A

S
x
G

A
|
A

R

A

L

I

L

V

A

N

A

H

S

H

A

D

V

I

R

D

A

K

F

V

S

A

F
|
F

T

T

G
|
G

S
|
S

T

T

E

V

V
x
T

G

G

R

K

I
x
Y

L

I

L

M

R

R

Q

Q

S

S

A

A

D

E

T

M

V

I

K

K

K

H

V

V

S

T

L

L

E
|
E

L
|
L

G

G

H

K

A

S

P

P

F

N

I

I

V

I

F

L

D

E

D

D

A

A

S

D

I

L

A

E

E

E

A

A

V

I

E

N

G

G

C

A

I

F

G

Q

A

G

K

I

F

M

A

Y

T

N

S

H

G

G

Q

Q

D

N

C
|
C

L

S

G

A

A

G

N

S

R

R

I

V

Y

F

V

V

H

H

R

R

N

K

I

H

Y

Y

G

E

Q

T

F

V

V

V

E

D

E

A

F

L

T

V

K

K

A

M

T

A

E

N

K

N

L

V

R

K

V

L

G

G

H

A

G

G

L

M

E

E

E

K

G

E

V

T

D

E

I

M

G

G

P

P

M

L

T

-

R

-

V

-

T

-

V

V

A

S

N

K

K

K

C
x
Q

R

Q

E

E

Q
x
R

I

V

S

L

N

N

A

Y

L

I

S

E

L

Q

G

G

A

K

K

K

-

E

-

G

-

A

-

T

L

V

T

A

C

A

G

G

I

E

D

R

N

A

H

L

L

E

G

K

S

G

S

Y

F

F

V

V

L

K

P

P

T

T

V

V

L

F

A

T

D

D

V

V

T

T

D

D

K

D

M

M

D

T

I

I

A

V

F

K

E

E

E
|
E

T

I

F
|
F

G

G

P

P

V

V

A

V

I

V

L

L

P

P

F

F

D

D

S

S

E

T

D

E

E

E

V

V

V

I

T

E

R

R

A

A

N

N

T

S

E

S

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

W

T

T

N

Q

D

N

L

I

R

K

R

T

A

G

C

H

R

Q

V

V

S

A

D

N

R

K

L

L

E

K

Y

A

G

G

M

T

V

V

A

W

L

I

N

N

T

D

P

Y

K

N

F

L

T

E

G

N

A

A

P

A

I

A

P

P

F

F

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

I

G

G

R

R

E
|
E

G

L

S

G

K

S

H

Y

G

A

L

L

A

D

E

N

Y

Y

M

T

E

E

L

V

K

K

Y

S

V

V

C

W

I

V

active site: N165 (= N164), K188 (= K187), E263 (= E262), C297 (= C296), E394 (= E393), E471 (= E470)
binding nicotinamide-adenine-dinucleotide: I161 (= I160), I162 (≠ T161), P163 (= P162), W164 (= W163), K188 (= K187), E191 (≠ P190), G221 (≠ A220), G225 (≠ S224), A226 (= A225), F239 (= F238), G241 (= G240), S242 (= S241), T245 (≠ V244), Y248 (≠ I247), L264 (= L263), C297 (= C296), Q344 (≠ C347), R347 (≠ Q350), E394 (= E393), F396 (= F395)

4go2A Crystal structure of thE C-terminal domain of 10'formyltetrahydrofolate dehydrogenase in complex with thio-NADP (see paper)
39% identity, 98% coverage: 8:485/490 of query aligns to 8:494/498 of 4go2A

query
sites
4go2A

A

A

L

V

N

N

K

K

L

L

R

T

R

L

N

Q

D

-

L

-

L

M

E

P

T

Y

R

Q

A

L

Y

F

I

I

D

G

G

G

R

E

W

F

V

V

T

D

G

A

E

E

G

G

S

S

-

K

-

T

L

Y

D

N

V

T

T

I

D

N

P

P

A

T

N

D

D

G

Q

S

V

V

I

I

A

C

Q

Q

V

V

A

S

H

L

C

A

D

Q

E

V

S

S

W

D

V

V

D

D

L

K

A

A

V

V

E

A

A

A

S

K

R

E

A

A

F

F

D

E

G

N

-

G

-

L

W

W

R

G

N

K

T

I

L

N

P

A

T

R

Q

D

R

R

G

G

A

R

V

L

L

L

R

Y

K

R

W

L

A

A

A

D

L

V

M

M

R

E

E

Q

N

H

Q

Q

E

E

D

E

L

L

A

A

V

T

I

I

M

E

T

A

C

L

E

D

Q

A

G

G

K

A

P

V

L

Y

S

T

E

L

S

A

-

L

R

K

G

T

E

H

I

V

N

G

Y

M

G

S

A

I

N

Q

F

T

V

F

E

R

W

Y

F

F

A

A

A

G

E

W

G

C

E

D

R

K

C

I

Y

Q

G

G

E

A

T

T

I

I

P

P

S

I

H

N

L

Q

-

A

-

R

P

P

N

N

S

R

Q

N

L

L

-

T

V

L

T

T

K

K

L

K

Q

E

P

P

I

V

G

G

V

V

T

C

V

G

A

I

I
x
V

T
x
I

P
|
P

W
|
W

N
|
N

F

Y

P

P

S

L

A

M

M

M

I

L

T

S

R

W

K

K

A

T

A

A

A

C

L

L

A

A

G

G

C

N

P

T

M

V

I

V

V

I

K
|
K

P

P

A
|
A

P
x
Q

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

F

A

A

R

E

L

L

A

T

E

L

E

K

A

A

G

G

I

I

P

P

G

K

G

G

V

V

F

V

Q

N

V

I

L

L

T

P

G

G

D
x
S

A
x
G

P

S

K

L

M

V

S
x
G

A
x
Q

R

R

L

L

L

S

A

D

A

H

S

P

A

D

V

V

R

R

A

K

F

I

S

G

F
|
F

T

T

G
|
G

S
|
S

T

T

E

E

V
|
V

G

G

R

K

I

H

L
x
I

L

M

R

K

Q

S

S

C

A

A

-

L

D

S

T

N

V

V

K

K

V

V

S

S

L

L

E
|
E

L

L

G
|
G

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

A

D

D

A

C

S

D

I

L

A

N

E

K

A

A

V

V

E

Q

G

M

C

G

I

M

G

S

A

S

K

V

F

F

A

F

T

N

S

K

G

G

Q

E

D

N

C
|
C

L

I

G

A

A

A

N

G

R

R

I

L

Y

F

V

V

H

E

R

E

N

S

I

I

Y

H

G

N

Q

Q

F

F

V

V

E

Q

E

K

F

V

T

V

K

E

A

E

T

V

E

E

K

K

L

M

R

K

V

I

G

G

H

N

G

P

L

L

E

E

E

R

G

D

V

T

D

N

I

H

G

G

P

P

M

Q

T

N

R

H

V

E

T

A

V

H

A

L

N

R

K

K

C

L

R

V

E

E

Q

Y

I

C

S

Q

N

R

A

G

L

V

S

K

L

E

G

G

A

A

K

T

L

L

T

V

C

C

G

G

I

N

D

Q

N

V

H

P

L

R

G

P

S

G

S

F

F

F

V

F

L

Q

P

P

T

T

V

V

L

F

A

T

D

D

V

V

T

E

D

D

K

H

M

M

D

Y

I

I

A

A

F

K

E

E

E
|
E

T

S

F
|
F

G

G

P

P

V

I

A

M

A

I

I

I

L

S

P

R

F

F

-

A

D

D

S

G

E

D

-

V

D

D

E

A

V

V

V

L

T

S

R

R

A

A

N

N

N

A

T

T

E

E

F

F

G

G

L

L

A

A

A

S

Y

G

V

V

Y

F

T

T

N

R

D

D

L

I

R

N

R

K

A

A

C

L

R

Y

V

V

S

S

D

D

R

K

L

L

E

Q

Y

A

G

G

M

T

V

V

A

F

L

I

N

N

T

T

P

Y

K

N

F

K

T

T

G

D

A

V

P

A

I

A

P

P

F

F

G

G

W

F

K

K

Q

Q

S

S

G

G

L

F

G

G

R

K

E
x
D

G

L

S

G

K

E

H

A

G

A

L

L

A

N

E

E

Y

Y

M

L

E

R

L

I

K

K

Y

T

V

V

active site: N170 (= N164), K193 (= K187), E269 (= E262), C303 (= C296), E400 (= E393), D479 (≠ E470)
binding 7-thionicotinamide-adenine-dinucleotide phosphate: V166 (≠ I160), I167 (≠ T161), P168 (= P162), W169 (= W163), K193 (= K187), A195 (= A189), Q196 (≠ P190), S225 (≠ D219), G226 (≠ A220), G230 (≠ S224), Q231 (≠ A225), F244 (= F238), G246 (= G240), S247 (= S241), V250 (= V244), I254 (≠ L248), E269 (= E262), G271 (= G264), C303 (= C296), E400 (= E393), F402 (= F395)

2o2rA Crystal structure of thE C-terminal domain of rat 10'formyltetrahydrofolate dehydrogenase in complex with NADPH (see paper)
39% identity, 98% coverage: 8:485/490 of query aligns to 8:494/498 of 2o2rA

query
sites
2o2rA

A

A

L

V

N

N

K

K

L

L

R

T

R

L

N

Q

D

-

L

-

L

M

E

P

T

Y

R

Q

A

L

Y

F

I

I

D

G

G

G

R

E

W

F

V

V

T

D

G

A

E

E

G

G

S

S

-

K

-

T

L

Y

D

N

V

T

T

I

D

N

P

P

A

T

N

D

D

G

Q

S

V

V

I

I

A

C

Q

Q

V

V

A

S

H

L

C

A

D

Q

E

V

S

S

W

D

V

V

D

D

L

K

A

A

V

V

E

A

A

A

S

K

R

E

A

A

F

F

D

E

G

N

-

G

-

L

W

W

R

G

N

K

T

I

L

N

P

A

T

R

Q

D

R

R

G

G

A

R

V

L

L

L

R

Y

K

R

W

L

A

A

A

D

L

V

M

M

R

E

E

Q

N

H

Q

Q

E

E

D

E

L

L

A

A

V

T

I

I

M

E

T

A

C

L

E

D

Q

A

G

G

K

A

P

V

L

Y

S

T

E

L

S

A

-

L

R

K

G

T

E

H

I

V

N

G

Y

M

G

S

A

I

N

Q

F

T

V

F

E

R

W

Y

F

F

A

A

A

G

E

W

G

C

E

D

R

K

C

I

Y

Q

G

G

E

A

T

T

I

I

P

P

S

I

H

N

L

Q

-

A

-

R

P

P

N

N

S

R

Q

N

L

L

-

T

V

L

T

T

K

K

L

K

Q

E

P

P

I

V

G

G

V

V

T

C

V

G

A

I

I
x
V

T
x
I

P

P

W
|
W

N
|
N

F

Y

P

P

S

L

A

M

M

M

I

L

T

S

R

W

K

K

A

T

A

A

A

C

L

L

A

A

G

G

C

N

P

T

M

V

I

V

V

I

K
|
K

P

P

A
|
A

P
x
Q

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

F

A

A

R

E

L

L

A

T

E

L

E

K

A

A

G

G

I

I

P

P

G

K

G

G

V

V

F

V

Q

N

V

I

L

L

T

P

G

G

D
x
S

A
x
G

P

S

K

L

M

V

S
x
G

A
x
Q

R

R

L

L

L

S

A

D

A

H

S

P

A

D

V

V

R

R

A

K

F

I

S

G

F
|
F

T

T

G

G

S
|
S

T

T

E

E

V
|
V

G

G

R

K

I

H

L
x
I

L

M

R

K

Q

S

S

C

A

A

-

L

D

S

T

N

V

V

K

K

V

V

S

S

L

L

E
|
E

L

L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

A

D

D

A

C

S

D

I

L

A

N

E

K

A

A

V

V

E

Q

G

M

C

G

I

M

G

S

A

S

K

V

F

F

A

F

T

N

S

K

G

G

Q

E

D

N

C
|
C

L

I

G

A

A

A

N

G

R

R

I

L

Y

F

V

V

H

E

R

E

N

S

I

I

Y

H

G

N

Q

Q

F

F

V

V

E

Q

E

K

F

V

T

V

K

E

A

E

T

V

E

E

K

K

L

M

R

K

V

I

G

G

H

N

G

P

L

L

E

E

E

R

G

D

V

T

D

N

I

H

G

G

P

P

M

Q

T

N

R

H

V

E

T

A

V

H

A

L

N

R

K

K

C

L

R

V

E

E

Q

Y

I

C

S

Q

N

R

A

G

L

V

S

K

L

E

G

G

A

A

K

T

L

L

T

V

C

C

G

G

I

N

D

Q

N

V

H

P

L

R

G

P

S

G

S

F

F

F

V

F

L

Q

P

P

T

T

V

V

L

F

A

T

D

D

V

V

T

E

D

D

K

H

M

M

D

Y

I

I

A

A

F

K

E

E

E
|
E

T

S

F

F

G

G

P

P

V

I

A

M

A

I

I

I

L

S

P

R

F

F

-

A

D

D

S

G

E

D

-

V

D

D

E

A

V

V

V

L

T

S

R

R

A

A

N

N

N

A

T

T

E

E

F

F

G

G

L

L

A

A

A

S

Y

G

V

V

Y

F

T

T

N

R

D

D

L

I

R

N

R

K

A

A

C

L

R

Y

V

V

S

S

D

D

R

K

L

L

E

Q

Y

A

G

G

M

T

V

V

A

F

L

I

N

N

T

T

P

Y

K

N

F

K

T

T

G

D

A

V

P

A

I

A

P

P

F

F

G

G

W

F

K

K

Q

Q

S

S

G

G

L

F

G

G

R

K

E
x
D

G

L

S

G

K

E

H

A

G

A

L

L

A

N

E

E

Y

Y

M

L

E

R

L

I

K

K

Y

T

V

V

active site: N170 (= N164), K193 (= K187), E269 (= E262), C303 (= C296), E400 (= E393), D479 (≠ E470)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V166 (≠ I160), I167 (≠ T161), W169 (= W163), K193 (= K187), A195 (= A189), Q196 (≠ P190), S225 (≠ D219), G226 (≠ A220), G230 (≠ S224), Q231 (≠ A225), F244 (= F238), S247 (= S241), V250 (= V244), I254 (≠ L248)

7rluA Structure of aldh1l1 (10-formyltetrahydrofolate dehydrogenase) in complex with NADP (see paper)
39% identity, 98% coverage: 8:485/490 of query aligns to 93:579/583 of 7rluA

query
sites
7rluA

A

A

L

V

N

N

K

K

L

L

R

T

R

L

N

Q

D

-

L

-

L

M

E

P

T

Y

R

Q

A

L

Y

F

I

I

D

G

G

G

R

E

W

F

V

V

T

D

G

A

E

E

G

G

S

S

-

K

-

T

L

Y

D

N

V

T

T

I

D

N

P

P

A

T

N

D

D

G

Q

S

V

V

I

I

A

C

Q

Q

V

V

A

S

H

L

C

A

D

Q

E

V

S

S

W

D

V

V

D

D

L

K

A

A

V

V

E

A

A

A

S

K

R

E

A

A

F

F

D

E

G

N

-

G

-

L

W

W

R

G

N

K

T

I

L

N

P

A

T

R

Q

D

R

R

G

G

A

R

V

L

L

L

R

Y

K

R

W

L

A

A

A

D

L

V

M

M

R

E

E

Q

N

H

Q

Q

E

E

D

E

L

L

A

A

V

T

I

I

M

E

T

A

C

L

E

D

Q

A

G

G

K

A

P

V

L

Y

S

T

E

L

S

A

-

L

R
x
K

G

T

E

H

I

V

N

G

Y

M

G

S

A

I

N

Q

F

T

V

F

E

R

W

Y

F

F

A

A

A

G

E

W

G

C

E

D

R

K

C

I

Y

Q

G

G

E

A

T

T

I

I

P

P

S

I

H

N

L

Q

-

A

-

R

P

P

N

N

S

R

Q

N

L

L

-

T

V

L

T

T

K

K

L

K

Q

E

P

P

I

V

G

G

V

V

T

C

V

G

A

I

I

V

T

I

P

P

W

W

N

N

F

Y

P

P

S

L

A

M

M

M

I

L

T

S

R

W

K

K

A

T

A

A

A

C

L

L

A

A

G

G

C

N

P

T

M

V

I

V

V

I

K
|
K

P

P

A

A

P

Q

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

F

A

A

R

E

L

L

A

T

E

L

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K

A

A

G

G

I

I

P

P

G

K

G

G

V

V

F

V

Q

N

V

I

L

L

T

P

G

G

D
x
S

A
x
G

P

S

K

L

M

V

S
x
G

A

Q

R

R

L

L

L

S

A

D

A

H

S

P

A

D

V

V

R

R

A

K

F

I

S

G

F

F

T

T

G
|
G

S
|
S

T

T

E

E

V
|
V

G

G

R

K

I

H

L

I

L

M

R

K

Q

S

S

C

A

A

-

L

D

S

T

N

V

V

K

K

V

V

S

S

L

L

E

E

L

L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

A

D

D

A

C

S

D

I

L

A

N

E

K

A

A

V

V

E

Q

G

M

C

G

I

M

G

S

A

S

K

V

F

F

A
x
F

T

N

S

K

G

G

Q

E

D
x
N

C
|
C

L

I

G

A

A

A

N

G

R

R

I

L

Y

F

V

V

H

E

R

E

N

S

I

I

Y

H

G

N

Q

Q

F

F

V

V

E

Q

E

K

F

V

T

V

K

E

A

E

T

V

E

E

K

K

L

M

R

K

V

I

G

G

H

N

G

P

L

L

E

E

E

R

G

D

V

T

D

N

I

H

G

G

P

P

M

Q

T

N

R

H

V

E

T

A

V

H

A

L

N

R

K

K

C

L

R

V

E

E

Q

Y

I

C

S

Q

N

R

A

G

L

V

S

K

L

E

G

G

A

A

K

T

L

L

T

V

C

C

G

G

I

N

D

Q

N

V

H

P

L

R

G

P

S

G

S

F

F

F

V

F

L

Q

P

P

T

T

V

V

L

F

A

T

D

D

V

V

T

E

D

D

K

H

M

M

D

Y

I

I

A

A

F

K

E

E

T

S

F

F

G

G

P

P

V

I

A

M

A

I

I

I

L

S

P

R

F

F

-

A

D

D

S

G

E

D

-

V

D

D

E

A

V

V

V

L

T

S

R

R

A

A

N

N

N

A

T

T

E

E

F

F

G

G

L

L

A

A

A

S

Y

G

V

V

Y

F

T

T

N

R

D

D

L

I

R

N

R

K

A

A

C

L

R

Y

V

V

S

S

D

D

R

K

L

L

E

Q

Y

A

G

G

M

T

V

V

A

F

L

I

N

N

T

T

P

Y

K
x
N

F

K

T

T

G

D

A

V

P

A

I

A

P

P

F

F

G

G

W

F

K

K

Q

Q

S

S

G

G

L

F

G

G

R

K

E

D

G

L

S

G

K

E

H

A

G

A

L

L

A

N

E

E

Y

Y

M

L

E

R

L

I

K

K

Y

T

V

V

binding nadp nicotinamide-adenine-dinucleotide phosphate: K278 (= K187), S310 (≠ D219), G311 (≠ A220), G315 (≠ S224), G331 (= G240), S332 (= S241), V335 (= V244)
binding 4'-phosphopantetheine: K201 (≠ R113), F382 (≠ A290), N387 (≠ D295), C388 (= C296), N545 (≠ K451)

Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
38% identity, 96% coverage: 19:487/490 of query aligns to 6:481/490 of Q9HTJ1

query
sites
Q9HTJ1

E

E

T

Q

R

K

A

L

Y

Y

I

I

D

G

G

G

R

R

W

Y

V

V

T

E

G

A

E

S

-

S

-

G

G

A

S

T

L

F

D

E

V

T

T

I

D

N

P

P

A

A

N

N

D

G

Q

E

V

V

I

L

A

A

Q

K

V

V

A

Q

H

R

C

A

D

S

E

R

S

E

W

D

V

V

D

E

L

R

A

A

V

V

E

Q

A

S

A

A

S

V

R

E

A

G

F

Q

D

K

G

V

W

W

R

A

N

A

T

M

L

T

P

A

T

M

Q

Q

R

R

G

S

A

R

V

I

L

L

R

R

K

R

W

A

A

V

A

D

L

I

M

L

R

R

E

E

N

R

Q

N

E

D

D

E

L

L

A

A

V

A

I

L

M

E

T

T

C

L

E

D

Q

T

G

G

K

K

P

P

L

L

S

A

E

E

S

T

R

R

G

S

-

V

E

D

I

I

N

V

Y

T

G

G

A

A

N

D

F

V

V

L

E

E

W

Y

F

Y

A

A

A

G

E

L

G

V

E

P

R

A

C

I

Y

E

G

G

E

E

T

Q

I

I

P

P

S

L

H

R

L

-

P

E

N

T

S

S

Q

F

L

V

V

Y

T

T

K

R

L

R

Q

E

P

P

I

L

G

G

V

V

T

V

V

A

A

G

I

I

T
x
G

P
x
A

W
|
W

N
|
N

F

Y

P

P

S

V

A

Q

M

I

I

A

T

L

R

W

K
|
K

A

S

A

A

A

P

A

A

L

L

A

A

G

G

C

N

P

A

M

M

I

I

V

F

K
|
K

P
|
P

A
x
S

P
x
E

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

L

A

A

R

E

L

I

A

Y

E

T

E

E

A

A

G

G

I

V

P

P

G

D

G

G

V

V

F

F

Q

N

V

V

L

L

T

T

G

G

D

S

A
x
G

P

R

K

E

M

V

S

G

A

Q

R

W

L

L

L

T

A

E

A

H

S

P

A

L

V

I

R

E

A

K

F

I

S

S

F

F

T

T

G

G

S
x
G

T
|
T

E
x
S

V
x
T

G

G

R

K

-

K

I

V

L

M

L

A

R

S

Q

A

S

S

A

S

D

S

T

S

V

L

K

K

K

E

V

V

S

T

L

M

E
|
E

L

L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

P

D

D

A

A

S

D

I

L

A

D

E

R

A

A

V

A

E

D

G

I

C

A

I

V

G

M

A

A

K

N

F

F

A

F

T

S

S

S

G

G

Q

Q

D

V

C
|
C

L

T

G

N

A

G

N

T

R

R

I

V

Y

F

V

I

H

H

R

R

N

S

I

Q

Y

Q

G

A

Q

R

F

F

V

E

E

A

E

K

F

V

T

L

K

E

A

R

T

V

E

Q

K

R

L

I

R

R

V

L

G

G

H

D

G

P

L

Q

E

D

E

E

G

N

V

T

D

N

I

F

G

G

P

P

M

L

T

V

R

S

V

F

T

P

V

H

A

M

N

E

K

S

C

V

R

L

E

G

Q

Y

I

I

S

E

N

S

A

G

L

K

S

A

L

Q

G

K

A

A

K

R

L

L

T

L

C

C

G

G

-

E

-

R

-

V

I

T

D

D

N

G

H

A

L

F

G

G

S

K

-

G

S

A

F

Y

V

V

L

A

P

P

T

T

V

V

L

F

A

T

D

D

V

C

T

R

D

D

K

D

M

M

D

T

I

I

A

V

F

R

E

E

E
|
E

T

I

F

F

G

G

P

P

V

V

A

M

A

S

I

I

L

L

P

V

F

Y

D

D

S

D

E

E

D

D

E

E

V

A

V

I

T

R

R

R

A

A

N

N

N

D

T

T

E

E

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

V

T

T

N

Q

D

D

L

L

R

A

R

R

A

A

C

H

R

R

V

A

S

I

D

H

R

R

L

L

E

E

Y

A

G

G

M

I

V

C

A

W

L

I

N

N

T

T

P

W

K

G

F

E

T

S

G

P

A

A

P

E

I

M

P

P

F

V

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

G

N

S

G

K

L

H

T

G

T

L

L

A

A

E

H

Y

Y

M

T

E

R

L

I

K

K

Y

S

V

V

C

Q

I

V

GAWN 150:153 (≠ TPWN 161:164) binding NADPH
K162 (= K173) active site, Charge relay system
KPSE 176:179 (≠ KPAP 187:190) binding NADPH
G209 (≠ A220) binding NADPH
GTST 230:233 (≠ STEV 241:244) binding NADPH
E252 (= E262) active site, Proton acceptor
C286 (= C296) binding covalent; modified: Cysteine sulfenic acid (-SOH)
E387 (= E393) binding NADPH
E464 (= E470) active site, Charge relay system

4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
38% identity, 96% coverage: 19:487/490 of query aligns to 5:480/489 of 4cazA

query
sites
4cazA

E

E

T

Q

R

K

A

L

Y

Y

I

I

D

G

G

G

R

R

W

Y

V

V

T

E

G

A

E

S

-

S

-

G

G

A

S

T

L

F

D

E

V

T

T

I

D

N

P

P

A

A

N

N

D

G

Q

E

V

V

I

L

A

A

Q

K

V

V

A

Q

H

R

C

A

D

S

E

R

S

E

W

D

V

V

D

E

L

R

A

A

V

V

E

Q

A

S

A

A

S

V

R

E

A

G

F

Q

D

K

G

V

W

W

R

A

N

A

T

M

L

T

P

A

T

M

Q

Q

R

R

G

S

A

R

V

I

L

L

R

R

K

R

W

A

A

V

A

D

L

I

M

L

R

R

E

E

N

R

Q

N

E

D

D

E

L

L

A

A

V

A

I

L

M

E

T

T

C

L

E

D

Q

T

G

G

K

K

P

P

L

L

S

A

E

E

S

T

R

R

G

S

-

V

E

D

I

I

N

V

Y

T

G

G

A

A

N

D

F

V

V

L

E

E

W

Y

F

Y

A

A

A

G

E

L

G

V

E

P

R

A

C

I

Y

E

G

G

E

E

T

Q

I

I

P

P

S

L

H

R

L

-

P

E

N

T

S

S

Q

F

L

V

V

Y

T

T

K

R

L

R

Q

E

P

P

I

L

G

G

V

V

T

V

V

A

A

G

I
|
I

T
x
G

P

A

W
|
W

N
|
N

F

Y

P

P

S

V

A

Q

M

I

I

A

T

L

R

W

K

K

A

S

A

A

A

P

A

A

L

L

A

A

G

G

C

N

P

A

M

M

I

I

V

F

K
|
K

P

P

A

S

P
x
E

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

L

A

A

R

E

L

I

A

Y

E

T

E

E

A

A

G

G

I

V

P

P

G

D

G

G

V

V

F

F

Q

N

V

V

L

L

T

T

G

G

D

S

A
x
G

P

R

K

E

M

V

S
x
G

A

Q

R

W

L

L

L

T

A

E

A

H

S

P

A

L

V

I

R

E

A

K

F

I

S

S

F
|
F

T
|
T

G
|
G

S
x
G

T

T

E

S

V
x
T

G

G

R

K

-

K

I
x
V

L

M

L

A

R

S

Q

A

S

S

A

S

D

S

T

S

V

L

K

K

K

E

V

V

S

T

L

M

E
|
E

L
|
L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

P

D

D

A

A

S

D

I

L

A

D

E

R

A

A

V

A

E

D

G

I

C

A

I

V

G

M

A

A

K

N

F

F

A

F

T

S

S

S

G

G

Q

Q

D

V

C
|
C

L

T

G

N

A

G

N

T

R

R

I

V

Y

F

V

I

H

H

R

R

N

S

I

Q

Y

Q

G

A

Q

R

F

F

V

E

E

A

E

K

F

V

T

L

K

E

A

R

T

V

E

Q

K

R

L

I

R

R

V

L

G

G

H

D

G

P

L

Q

E

D

E

E

G

N

V

T

D

N

I

F

G

G

P

P

M

L

T

V

R

S

V

F

T

P

V

H

A

M

N

E

K

S

C

V

R

L

E

G

Q

Y

I

I

S

E

N

S

A

G

L

K

S

A

L

Q

G

K

A

A

K

R

L

L

T

L

C

C

G

G

-

E

-

R

-

V

I

T

D

D

N

G

H

A

L

F

G

G

S

K

-

G

S

A

F

Y

V

V

L

A

P

P

T

T

V

V

L

F

A

T

D

D

V

C

T

R

D

D

K

D

M

M

D

T

I

I

A

V

F

R

E

E

E
|
E

T

I

F
|
F

G

G

P

P

V

V

A

M

A

S

I

I

L

L

P

V

F

Y

D

D

S

D

E

E

D

D

E

E

V

A

V

I

T

R

R

R

A

A

N

N

N

D

T

T

E

E

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

V

T

T

N

Q

D

D

L

L

R

A

R

R

A

A

C

H

R

R

V

A

S

I

D

H

R

R

L

L

E

E

Y

A

G

G

M

I

V

C

A

W

L

I

N

N

T

T

P

W

K

G

F

E

T

S

G

P

A

A

P

E

I

M

P

P

F

V

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

G

N

S

G

K

L

H

T

G

T

L

L

A

A

E

H

Y

Y

M

T

E

R

L

I

K

K

Y

S

V

V

C

Q

I

V

active site: N152 (= N164), K175 (= K187), E251 (= E262), C285 (= C296), E386 (= E393), E463 (= E470)
binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I160), G149 (≠ T161), W151 (= W163), N152 (= N164), K175 (= K187), E178 (≠ P190), G208 (≠ A220), G212 (≠ S224), F226 (= F238), T227 (= T239), G228 (= G240), G229 (≠ S241), T232 (≠ V244), V236 (≠ I247), E251 (= E262), L252 (= L263), C285 (= C296), E386 (= E393), F388 (= F395)

2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
38% identity, 96% coverage: 19:487/490 of query aligns to 5:480/489 of 2woxA

query
sites
2woxA

E

E

T

Q

R

K

A

L

Y

Y

I

I

D

G

G

G

R

R

W

Y

V

V

T

E

G

A

E

S

-

S

-

G

G

A

S

T

L

F

D

E

V

T

T

I

D

N

P

P

A

A

N

N

D

G

Q

E

V

V

I

L

A

A

Q

K

V

V

A

Q

H

R

C

A

D

S

E

R

S

E

W

D

V

V

D

E

L

R

A

A

V

V

E

Q

A

S

A

A

S

V

R

E

A

G

F

Q

D

K

G

V

W

W

R

A

N

A

T

M

L

T

P

A

T

M

Q

Q

R

R

G

S

A

R

V

I

L

L

R

R

K

R

W

A

A

V

A

D

L

I

M

L

R

R

E

E

N

R

Q

N

E

D

D

E

L

L

A

A

V

A

I

L

M

E

T

T

C

L

E

D

Q

T

G

G

K

K

P

P

L

L

S

A

E

E

S

T

R

R

G

S

-

V

E

D

I

I

N

V

Y

T

G

G

A

A

N

D

F

V

V

L

E

E

W

Y

F

Y

A

A

A

G

E

L

G

V

E

P

R

A

C

I

Y

E

G

G

E

E

T

Q

I

I

P

P

S

L

H

R

L

-

P

E

N

T

S

S

Q

F

L

V

V

Y

T

T

K

R

L

R

Q

E

P

P

I

L

G

G

V

V

T

V

V

A

A

G

I
|
I

T
x
G

P

A

W
|
W

N
|
N

F

Y

P

P

S

V

A

Q

M

I

I

A

T

L

R

W

K

K

A

S

A

A

A

P

A

A

L

L

A

A

G

G

C

N

P

A

M

M

I

I

V

F

K
|
K

P

P

A
x
S

P
x
E

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

L

A

A

R

E

L

I

A

Y

E

T

E

E

A

A

G

G

I

V

P

P

G

D

G

G

V

V

F

F

Q

N

V

V

L

L

T

T

G

G

D

S

A
x
G

P

R

K

E

M

V

S
x
G

A

Q

R

W

L

L

L

T

A

E

A

H

S

P

A

L

V

I

R

E

A

K

F

I

S

S

F
|
F

T
|
T

G
|
G

S
x
G

T

T

E

S

V
x
T

G

G

R

K

-

K

I
x
V

L

M

L

A

R

S

Q

A

S

S

A

S

D

S

T

S

V

L

K

K

K

E

V

V

S

T

L

M

E
|
E

L
|
L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

P

D

D

A

A

S

D

I

L

A

D

E

R

A

A

V

A

E

D

G

I

C

A

I

V

G

M

A

A

K

N

F

F

A

F

T

S

S

S

G

G

Q

Q

D

V

C
|
C

L

T

G

N

A

G

N

T

R

R

I

V

Y

F

V

I

H

H

R

R

N

S

I

Q

Y

Q

G

A

Q

R

F

F

V

E

E

A

E

K

F

V

T

L

K

E

A

R

T

V

E

Q

K

R

L

I

R

R

V

L

G

G

H

D

G

P

L

Q

E

D

E

E

G

N

V

T

D

N

I

F

G

G

P

P

M

L

T

V

R

S

V

F

T

P

V

H

A

M

N

E

K

S

C

V

R

L

E

G

Q

Y

I

I

S

E

N

S

A

G

L

K

S

A

L

Q

G

K

A

A

K

R

L

L

T

L

C

C

G

G

-

E

-

R

-

V

I

T

D

D

N

G

H

A

L

F

G

G

S

K

-

G

S

A

F

Y

V

V

L

A

P

P

T

T

V

V

L

F

A

T

D

D

V

C

T

R

D

D

K

D

M

M

D

T

I

I

A

V

F

R

E

E

E
|
E

T

I

F
|
F

G

G

P

P

V

V

A

M

A

S

I

I

L

L

P

V

F

Y

D

D

S

D

E

E

D

D

E

E

V

A

V

I

T

R

R

R

A

A

N

N

N

D

T

T

E

E

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

V

T

T

N

Q

D

D

L

L

R

A

R

R

A

A

C

H

R

R

V

A

S

I

D

H

R

R

L

L

E

E

Y

A

G

G

M

I

V

C

A

W

L

I

N

N

T

T

P

W

K

G

F

E

T

S

G

P

A

A

P

E

I

M

P

P

F

V

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

G

N

S

G

K

L

H

T

G

T

L

L

A

A

E

H

Y

Y

M

T

E

R

L

I

K

K

Y

S

V

V

C

Q

I

V

active site: N152 (= N164), K175 (= K187), E251 (= E262), C285 (= C296), E386 (= E393), E463 (= E470)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I160), G149 (≠ T161), W151 (= W163), N152 (= N164), K175 (= K187), S177 (≠ A189), E178 (≠ P190), G208 (≠ A220), G212 (≠ S224), F226 (= F238), T227 (= T239), G228 (= G240), G229 (≠ S241), T232 (≠ V244), V236 (≠ I247), E251 (= E262), L252 (= L263), C285 (= C296), E386 (= E393), F388 (= F395)

2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
38% identity, 96% coverage: 19:487/490 of query aligns to 5:480/489 of 2wmeA

query
sites
2wmeA

E

E

T

Q

R

K

A

L

Y

Y

I

I

D

G

G

G

R

R

W

Y

V

V

T

E

G

A

E

S

-

S

-

G

G

A

S

T

L

F

D

E

V

T

T

I

D

N

P

P

A

A

N

N

D

G

Q

E

V

V

I

L

A

A

Q

K

V

V

A

Q

H

R

C

A

D

S

E

R

S

E

W

D

V

V

D

E

L

R

A

A

V

V

E

Q

A

S

A

A

S

V

R

E

A

G

F

Q

D

K

G

V

W

W

R

A

N

A

T

M

L

T

P

A

T

M

Q

Q

R

R

G

S

A

R

V

I

L

L

R

R

K

R

W

A

A

V

A

D

L

I

M

L

R

R

E

E

N

R

Q

N

E

D

D

E

L

L

A

A

V

A

I

L

M

E

T

T

C

L

E

D

Q

T

G

G

K

K

P

P

L

L

S

A

E

E

S

T

R

R

G

S

-

V

E

D

I

I

N

V

Y

T

G

G

A

A

N

D

F

V

V

L

E

E

W

Y

F

Y

A

A

A

G

E

L

G

V

E

P

R

A

C

I

Y

E

G

G

E

E

T

Q

I

I

P

P

S

L

H

R

L

-

P

E

N

T

S

S

Q

F

L

V

V

Y

T

T

K

R

L

R

Q

E

P

P

I

L

G

G

V

V

T

V

V

A

A

G

I

I

T
x
G

P

A

W
|
W

N
|
N

F

Y

P

P

S

V

A

Q

M

I

I

A

T

L

R

W

K

K

A

S

A

A

A

P

A

A

L

L

A

A

G

G

C

N

P

A

M

M

I

I

V

F

K
|
K

P

P

A
x
S

P
x
E

E

V

T

T

P

P

L

L

S

T

A

A

L

L

A

K

L

L

A

A

R

E

L

I

A

Y

E

T

E

E

A

A

G

G

I

V

P

P

G

D

G

G

V

V

F

F

Q

N

V

V

L

L

T

T

G

G

D

S

A
x
G

P

R

K

E

M

V

S
x
G

A

Q

R

W

L

L

L

T

A

E

A

H

S

P

A

L

V

I

R

E

A

K

F

I

S

S

F
|
F

T

T

G
|
G

S
x
G

T

T

E

S

V
x
T

G

G

R

K

-

K

I
x
V

L

M

L

A

R

S

Q

A

S

S

A

S

D

S

T

S

V

L

K

K

K

E

V

V

S

T

L

M

E
|
E

L

L

G

G

H

K

A

S

P

P

F

L

I

I

V

I

F

F

D

P

D

D

A

A

S

D

I

L

A

D

E

R

A

A

V

A

E

D

G

I

C

A

I

V

G

M

A

A

K

N

F

F

A

F

T

S

S

S

G

G

Q

Q

D

V

C
|
C

L

T

G

N

A

G

N

T

R

R

I

V

Y

F

V

I

H

H

R

R

N

S

I

Q

Y

Q

G

A

Q

R

F

F

V

E

E

A

E

K

F

V

T

L

K

E

A

R

T

V

E

Q

K

R

L

I

R

R

V

L

G

G

H

D

G

P

L

Q

E

D

E

E

G

N

V

T

D

N

I

F

G

G

P

P

M

L

T

V

R

S

V

F

T

P

V

H

A

M

N

E

K

S

C

V

R

L

E

G

Q

Y

I

I

S

E

N

S

A

G

L

K

S

A

L

Q

G

K

A

A

K

R

L

L

T

L

C

C

G

G

-

E

-

R

-

V

I

T

D

D

N

G

H

A

L

F

G

G

S

K

-

G

S

A

F

Y

V

V

L

A

P

P

T

T

V

V

L

F

A

T

D

D

V

C

T

R

D

D

K

D

M

M

D

T

I

I

A

V

F

R

E

E

E
|
E

T

I

F

F

G

G

P

P

V

V

A

M

A

S

I

I

L

L

P

V

F

Y

D

D

S

D

E

E

D

D

E

E

V

A

V

I

T

R

R

R

A

A

N

N

N

D

T

T

E

E

F

Y

G

G

L

L

A

A

Y

G

V

V

Y

V

T

T

N

Q

D

D

L

L

R

A

R

R

A

A

C

H

R

R

V

A

S

I

D

H

R

R

L

L

E

E

Y

A

G

G

M

I

V

C

A

W

L

I

N

N

T

T

P

W

K

G

F

E

T

S

G

P

A

A

P

E

I

M

P

P

F

V

G

G

W

Y

K

K

Q

Q

S

S

G

G

L

V

G

G

R

R

E
|
E

G

N

S

G

K

L

H

T

G

T

L

L

A

A

E

H

Y

Y

M

T

E

R

L

I

K

K

Y

S

V

V

C

Q

I

V

active site: N152 (= N164), K175 (= K187), E251 (= E262), C285 (= C296), E386 (= E393), E463 (= E470)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (≠ T161), W151 (= W163), K175 (= K187), S177 (≠ A189), E178 (≠ P190), G208 (≠ A220), G212 (≠ S224), F226 (= F238), G228 (= G240), G229 (≠ S241), T232 (≠ V244), V236 (≠ I247)

P28037 Cytosolic 10-formyltetrahydrofolate dehydrogenase; 10-FTHFDH; FDH; Aldehyde dehydrogenase family 1 member L1; FBP-CI; EC 1.5.1.6 from Rattus norvegicus (Rat) (see 5 papers)
39% identity, 98% coverage: 7:485/490 of query aligns to 411:898/902 of P28037

query
sites
P28037

S

K

A

A

L

V

N

N

K

K

L

L

R

T

R

L

N

Q

D

-

L

-

L

M

E

P

T

Y

R

Q

A

L

Y

F

I

I

D

G

G

G

R

E

W

F

V

V

T

D

G

A

E

E

G

G

S

S

-

K

-

T

L

Y

D

N

V

T

T

I

D

N

P

P

A

T

N

D

D

G

Q

S

V

V

I

I

A

C

Q

Q

V

V

A

S

H

L

C

A

D

Q

E

V

S

S

W

D

V

V

D

D

L

K

A

A

V

V

E

A

A

A

S

K

R

E

A

A

F

F

D

E

G

N

-

G

-

L

W

W

R

G

N

K

T

I

L

N

P

A

T

R

Q

D

R

R

G

G

A

R

V

L

L

L

R

Y

K

R

W

L

A

A

A

D

L

V

M

M

R

E

E

Q

N

H

Q

Q

E

E

D

E

L

L

A

A

V

T

I

I

M

E

T

A

C

L

E

D

Q

A

G

G

K

A

P

V

L

Y

S

T

E

L

S

A

-

L

R

K

G

T

E

H

I

V

N

G

Y

M

G

S

A

I

N

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IPW 571:573 (≠ TPW 161:163) binding NADP(+)
KPAQ 597:600 (≠ KPAP 187:190) binding NADP(+)
GSLVGQ 630:635 (≠ APKMSA 220:225) binding NADP(+)
GS 650:651 (= GS 240:241) binding NADP(+)
E673 (= E262) mutation to A: Loss of aldehyde dehydrogenase activity.
EL 673:674 (= EL 262:263) binding NADP(+)
C707 (= C296) mutation to A: Loss of formyltetrahydrofolate dehydrogenase activity. No effect on formyltetrahydrofolate hydrolase activity. No effect on NADP binding. No effect on homotetramerization.
K757 (= K346) binding NADP(+)
ESF 804:806 (≠ ETF 393:395) binding NADP(+)

Sites not aligning to the query:

142 Essential for catalytic activity; D→A: Loss of formyltetrahydrofolate dehydrogenase activity. Loss of formyltetrahydrofolate hydrolase activity. No effect on aldehyde dehydrogenase activity.
354 modified: O-(pantetheine 4'-phosphoryl)serine; S→A: Loss of phosphopantetheinylation. Loss of formyltetrahydrofolate dehydrogenase activity. No effect on hydrolase and aldehyde dehydrogenase activities in vitro.

O14293 Putative aldehyde dehydrogenase-like protein C9E9.09c; EC 1.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
37% identity, 93% coverage: 33:487/490 of query aligns to 38:495/503 of O14293

query
sites
O14293

G

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S248 (= S241) modified: Phosphoserine

Sites not aligning to the query:

501 modified: Phosphoserine

Query Sequence

>PP_4422 FitnessBrowser__Putida:PP_4422
MKDLAPSALNKLRRNDLLETRAYIDGRWVTGEGSLDVTDPANDQVIAQVAHCDESWVDLA
VEAASRAFDGWRNTLPTQRGAVLRKWAALMRENQEDLAVIMTCEQGKPLSESRGEINYGA
NFVEWFAAEGERCYGETIPSHLPNSQLVTKLQPIGVTVAITPWNFPSAMITRKAAAALAA
GCPMIVKPAPETPLSALALARLAEEAGIPGGVFQVLTGDAPKMSARLLAASAVRAFSFTG
STEVGRILLRQSADTVKKVSLELGGHAPFIVFDDASIAEAVEGCIGAKFATSGQDCLGAN
RIYVHRNIYGQFVEEFTKATEKLRVGHGLEEGVDIGPMTRVTVANKCREQISNALSLGAK
LTCGGIDNHLGSSFVLPTVLADVTDKMDIAFEETFGPVAAILPFDSEDEVVTRANNTEFG
LAAYVYTNDLRRACRVSDRLEYGMVALNTPKFTGAPIPFGGWKQSGLGREGSKHGLAEYM
ELKYVCIGGL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory