Comparing PP_4548 FitnessBrowser__Putida:PP_4548 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 2 hits to proteins with known functional sites (download)
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
24% identity, 84% coverage: 35:400/435 of query aligns to 24:374/383 of 5i39A
5hxwA L-amino acid deaminase from proteus vulgaris (see paper)
27% identity, 51% coverage: 35:257/435 of query aligns to 16:229/433 of 5hxwA
Sites not aligning to the query:
>PP_4548 FitnessBrowser__Putida:PP_4548
MNAALNNGPAQRAPSYYSATLNDHTAYPQLKGTVQVDVAIIGGGFTGVATAVELAERGLK
VAIVETNRIGWGASGRNGGQVTGSLSGDEAMRSQMRNHLGAEVDDFIWHLRWRGHQVIEQ
RVARYGIDCDLKRGHLHAAMKPSHMNELRAFEAEARRRGMGDQVQLLDRAAVAEHLQSPL
YLGALKNLRNLHLHPLNLCLGEARAAHGLGALIFENSEVLDIVHGPRPAVVTAHGRVEAR
QVMLAGDVYHKLEKRQLKGKIFPAMGGIVTTAPLGELAEQINPQDLAVYDCRFVLDYYRL
TADKRLLFGGGANYSGKDSRDIEGELRPCIERTFPALKGVPIEFQWSCAMGIVVNRIPQL
GKLSDNVWYCQGYSGHGIATSHIMGEIMAEALTGTLEKFDTFAQCRHVRVPMGDLLGNPL
LAAGMWYYQMLEKLR
Or try a new SitesBLAST search
SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory