SitesBLAST

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 73% coverage: 6:338/455 of query aligns to 68:397/534 of P53322

query
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P53322

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K283 (= K223) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
24% identity, 84% coverage: 29:410/455 of query aligns to 4:365/393 of 6e9oA

query
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6e9oA

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binding D-galactonic acid: Y21 (= Y44), F114 (= F137), Q141 (≠ S164), Q228 (≠ Y266), S320 (= S362), N343 (= N388)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
23% identity, 80% coverage: 64:428/455 of query aligns to 104:472/495 of Q9NRA2

query
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Q9NRA2

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K136 (≠ R96) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I141) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 156:157) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LV 229:230) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ ATIDR 231:235) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G296) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P302) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (≠ A325) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

8u3gA Structure of naag-bound sialin
23% identity, 85% coverage: 40:428/455 of query aligns to 15:411/427 of 8u3gA

query
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8u3gA

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R

-

I

-

M

L

F

L

T

G

W

F

G

G

L

I

V

L

A

G

T

T

A

A

M

V

A

L

F

T

V

L

Q

F

N

T

E

P

T

I

Q

A

-

A

-

D

-

L

-

G

-

V

-

G

-

P

F

L

Y

I

V

V

L

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F
|
F

P

P

G

A

V

M

I

H

Y

A

Y

M

F

W

T

S

R

S

W

W

L

A

P

P

A

P

A

L

E

E

R

R

G

S

K

K

A

L

I

L

A

S

I

I

F

S

L

Y

S

A

G

G

S

A

A

Q

L

L

A

G

S

T

L

V

I

I

S

S

G

L

P

P

L

L

A

S

G

G

A

I

L

I

M

C

Q

Y

I

Y

Q

M

G

-

L

-

G

-

L

-

H

-

G

N

W

W

Q

T

W

Y

M

V

L

F

F

Y

I

F

E

F

G

G

M

T

A

I

S

G

V

I

A

-

L

F

C

W

F

F

F

L

V

L

F

W

F

I

W

W

L

L

D

V

S

S

K

D

-

T

P

P

Q

Q

D

K

A

H

K

K

W

R

L

I

S

S

K

H

A

Y

E

E

Q

K

D

E

A

Y

L

I

V

L

A

S

T

S

I

L

D

R

R

N

E

Q

Q

L

Q

S

A

S

R

Q

E

K

A

S

I

V

G

P

A

W

V

V

R

P

P

-

S

-

A

-

W

-

N

-

L

I

L

L

K

K

D

S

R

L

Q

P

I

L

V

W

L

A

F

I

C

V

L

V

I

A

Y

H

F

F

C

S

I

Y

Q

N

L

W

T

T

I

F

Y

Y

A

T

A

L

T

L

F

T

W

L

L

L

P

P

S

T

I

Y

I

M

K

K

R

E

M

I

G

L

D

R

L

F

S

N

D

V

M

Q

Q

E

V

N

G

G

F

F

F

L

N

S

S

S

I

L

P

P

-

Y

-

L

-

G

-

S

W

W

L

L

I

C

S

M

I

I

L

L

A

S

M

G

Y

Q

A

A

F

A

A

D

A

N

G

L

S

R

A

A

R

K

W

W

K

N

F

F

Q

S

Q

T

-

L

-

C

-

V

-

R

-

I

-

F

-

S

-

L

-

I

A

G

W

M

V

I

A

G

G

P

A

A

L

V

L

F

V

L

A

V

A

A

T

A

G

G

M

-

F

F

M

I

S

G

T

C

T

D

G

Y

G

S

P

L

V

A

F

V

A

A

F

F

V

L

A

T

V

I

C

S

F

T

A

T

A

L

I

G

G

G

F

F

K

C

S

S

A

S

S

G

S

-

L

-

F

-

W

F

P
x
S

I

I

P

N

Q
x
H

G

L

Y

D

L

I

D

A

A

P

R

S

I

Y

A

A

A

G

A

I

V

L

I
x
L

A

G

L

I

I

T

N

N

S

T

V

F

G

A

N

T

L

I

G

P

G

G

F

M

V

V

A

G

P

P

T

V

T

I

F

A

G

K

L

S

L

L

-

T

-

P

E

D

Q

N

Q

T

T

V

G

G

S

E

I

W

Q

Q

G

T

G

V

L

F

Y

Y

G

I

L

A

A

A

V

A

T

I

S

N

V

V

L

F

A

G

A

A

I

I

binding aspartic acid: F15 (= F40), Y19 (= Y44), F118 (= F137)
binding glutamic acid: S350 (≠ P369), H353 (≠ Q372), L365 (≠ I384)

8u3hA Structure of fmoc-leu-oh bound sialin
23% identity, 85% coverage: 40:428/455 of query aligns to 15:409/425 of 8u3hA

query
sites
8u3hA

F

F

I

F

V

I

N

V

Y
|
Y

L

A

D

L

R

R

V

V

N

N

-

L

-

S

I

V

G

A

F

L

V

V

R

D

P

M

H

V

L

D

E

S

S

K

D

K

L

Y

G

Q

I

W

S

D

A

A

A

E

A

T

Y

Q

G

G

F

W

G

I

A

L

G

G

L

S

F

F

I

Y

G

G

Y

Y

A

I

L

I

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

V

L

A

Q

S

K

K

V

I

G

G

A

G

R

K

L

M

W

-

L

-

T

-

R

-

I

-

M

L

F

L

T

G

W

F

G

G

L

I

V

L

A

G

T

T

A

A

M

V

A

L

F

T

V

L

Q

F

N

T

E

P

T

I

Q

A

-

A

-

D

-

L

-

G

-

V

-

G

-

P

F

L

Y

I

V

V

L

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

V

M

I

H

Y

A

Y

M

F

W

T

S

R

S

W

W

L

A

P

P

A

P

A

L

E

E

R

R

G

S

K

K

A

L

I

L

A

S

I

I

F

S

L

Y

S

A

G

G

S

A

A

Q

L

L

A

G

S

T

L

V

I

I

S

S

G

L

P

P

L

L

A

S

G

G

A

I

L

I

M

C

Q

Y

I

Y

Q

M

G

-

L

-

G

-

L

-

H

-

G

N

W

W

Q

T

W

Y

M

V

L

F

F

Y

I

F

E

F

G

G

M

T

A

I

S

G

V

I

A

-

L

F

C

W

F

F

F

L

V

L

F

W

F

I

W

W

L

L

D

V

S

S

K

D

-

T

P

P

Q

Q

D

K

A

H

K

K

W

R

L

I

S

S

K

H

A

Y

E

E

Q

K

D

E

A

Y

L

I

V

L

A

S

T

S

I

L

D

R

R

N

E

Q

Q

L

Q

S

A

S

R

Q

E

K

A

S

I

V

G

P

A

W

V

V

R

P

P

-

S

-

A

-

W

-

N

-

L

I

L

L

K

K

D

S

R

L

Q

P

I

L

V

W

L

A

F

I

C

V

L

V

I

A

Y
x
H

F

F

C

S

I
x
Y

Q

N

L

W

T

T

I

F

Y
|
Y

A

T

A

L

T

L

F

T

W

L

L

L

P

P

S

T

I

Y

I

M

K

K

R

E

M

I

G

L

D

R

L

F

S

N

D

V

M

Q

Q

E

V

N

G

G

F

F

F

L

N

S

S

S

I

L

P

P

-

Y

-

L

-

G

-

S

W

W

L

L

I

C

S
x
M

I

I

L

L

A

S

M

G

Y

Q

A

A

F

A

A

D

A

N

G

L

S

R

A

A

R

K

W

W

K

N

F

F

Q

S

Q

T

-

L

-

C

-

V

-

R

-

I

-

F

-

S

-

L

-

I

A

G

W

M

V

I

A

G

G

P

A

A

L

V

L

F

V

L

A

V

A

A

T

A

G

G

M

-

F

F

M

I

S

G

T

C

T

D

G

Y

G

S

P

L

V

A

F

V

A

A

F

F

V

L

A

T

V

I

C

S

F

T

A

T

A

L

I

G

G

G

F

F

K

C

S
|
S

A

S

S

G

S

-

L

-

F

-

W

F

P

S

I

I

P

N

Q

H

G

L

Y

D

L

I

D

A

A

P

R

S

I

Y

A

A

A

G

A

I

V

L

I

L

A

G

L

I

I

T

N

N

S

T

V

F

G

A

N

T

L

I

G

P

G

G

F

M

V

V

A

G

P

P

T

V

T

I

F

A

G

K

L

S

L

L

-

T

-

P

E

D

Q

N

Q

T

T

V

G

G

S

E

I

W

Q

Q

G

T

G

V

L

F

Y

Y

G

I

L

A

A

A

V

A

T

I

S

N

V

V

L

F

A

G

A

A

I

I

binding leucine: M279 (≠ S306), S344 (= S362)
binding Fluorenylmethyloxycarbonyl chloride: Y19 (= Y44), H235 (≠ Y266), Y238 (≠ I269), Y243 (= Y274)

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
24% identity, 57% coverage: 64:323/455 of query aligns to 104:359/495 of Q8BN82

query
sites
Q8BN82

S

D

A

A

A

E

A

T

Y

Q

G

G

F

W

G

I

A

L

G

G

L

S

F

F

I

Y

G

G

Y

Y

A

I

L

V

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

Q

S

K

R

V

V

G

G

A

G

R

K

L

L

W

L

L

L

T

G

R

L

I

G

M

I

F

L

T

G

W

T

G

S

L

V

V

F

A

T

-

L

-

F

T

T

A

P

M

L

A

A

F

A

V

D

Q

L

N

G

E

V

T

V

Q

T

F

L

Y

V

V

V

L

L

R

R

F

A

L

L

L

E

G

G

V

L

A

G

E

E

A

G

G

V

F

T

F

F

P

P

G

A

V

M

I
x
H

Y

A

Y

M

F

W

T

S

R

S

W

W

L

A

P

P

A

P

A

L

E

E

R

R

G

S

K

K

A

L

I

L

A

T

I

I

F

S

L

Y

S

A

G

G

S

A

A

Q

L

L

A

G

S

T

L

V

I

I

S

S

G

L

P

P

L

L

A

S

G

G

A

I

L

I

M

C

Q

Y

I

Y

Q

M

G

-

L

-

G

-

L

-

H

-

G

N

W

W

Q

T

W

Y

M

V

L

F

F

Y

I

L

E

F

G

G

M

I

A

V

S

G

V

I

A

-

L

V

C

W

F

F

F

I

V

L

F

W

F

M

W

W

L

I

D

V

S

S

K

D

-

T

P

P

Q

E

D

T

A

H

K

K

W

T

L

I

S

S

K

H

A

Y

E

E

Q

K

D

E

A

Y

L

I

V

V

A

S

T

S

I

L

D

K

R

N

E

Q

Q

L

Q

S

A

S

R

Q

E

K

A

V

I

V

G

-

A

-

V

-

R

-

P

-

S

-

A

P

W

W

-

G

N

S

L

I

L

L

K

K

D

S

R

L

Q

P

I

L

V

W

L

A

F

I

C

V

L

V

I

A

Y

H

F

F

C

S

I

Y

Q

N

L

W

T

S

I

F

Y

Y

A

T

A

L

T

L

F

T

W

L

L

L

P

P

S

T

I

Y

I

M

K

K

R

E

M

I

G

L

D

R

L

F

S

N

D

V

M

Q

Q

E

V

N

G

G

F

F

F

L

N

S

S

A

I

L

P

P

-

Y

-

F

-

G

-

C

W

W

L

L

I

C

S

M

I

I

L

L

A

C

M

G

Y

Q

A

A

F

A

A

D

A

Y

G

L

S

R

A

V

R

K

W

W

K

N

F

F

Q

S

H183 (≠ I141) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 57% coverage: 64:323/455 of query aligns to 104:359/495 of Q5Q0U0

query
sites
Q5Q0U0

S

D

A

A

A

E

A

T

Y

Q

G

G

F

W

G

I

A

L

G

G

L

S

F

F

I

Y

G

G

Y

Y

A

I

L

V

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

M

L

A

Q

S

K

R

V

I

G

G

A

G

R
x
K

L

L

W

L

L

L

T

G

R

L

I

G

M

I

F

L

T

G

W

T

G

A

L

V

V

F

A

T

-

L

-

F

T

T

A

P

M

L

A

A

F

A

V

D

Q

L

N

G

E

V

T

V

Q

A

F

L

Y

I

V

V

L

L

R
|
R

F

A

L

L

L
x
E

G
|
G

V

L

A

G

E
|
E

A
x
G

G

V

F

T

F
|
F

P
|
P

G

A

V

M

I
x
H

Y

A

Y

M

F
x
W

T

S

R

S

W

W

L

A

P

P

A

P

A

L

E

E

R

R

G

S

K

K

A

L

I

L

A

T

I

I

F

S

L

Y

S

A

G

G

S

A

A

Q

L

L

A

G

S

T

L

V

I

I

S

S

G

L

P

P

L

L

A

S

G

G

A

I

L

I

M

C

Q

Y

I

Y

Q

M

G

-

L

-

G

-

L

-

H

-

G

N

W

W

Q

T

W

Y

M

V

L

F

F

Y

I

L

E

F

G

G

M

L

A

V

S

G

V

I

A

-

L

V

C

W

F

F

F

I

V

L

F

W

F

M

W

W

L

I

D

V

S

S

K

D

-

T

P

P

Q

E

D

T

A

H

K

K

W

T

L

I

S

S

K

H

A

Y

E

E

Q

K

D

E

A

Y

L

I

V

V

A
x
S

T
x
S

I
x
L

D
x
K

R
x
N

E

Q

Q

L

Q

S

A

S

R

Q

E

K

A

V

I

V

G

P

A

W

V

V

R

-

P

-

S

-

A

-

W

-

N

S

L

I

L

L

K

K

D

S

R

L

Q

P

I

L

V

W

L

A

F

I

C

V

L

V

I

A

Y

H

F

F

C

S

I

Y

Q

N

L

W

T

T

I

F

Y

Y

A

T

A

L

T

L

F

T

W

L

L

L

P

P

S

T

I

Y

I

M

K

K

R

E

M

I

G

L

D

R

L

F

S

N

D

V

M

Q

Q

E

V

N

G

G

F

F

F

L

N

S

S

A

I

L

P
|
P

-

Y

-

F

-

G

-

C

W

W

L

L

I

C

S

M

I

I

L

V

A

C

M

G

Y

Q

A

A

F

A

A

D

A

Y

G

L

S

R

A

V

R

K

W

W

K

N

F

F

Q

S

K136 (≠ R96) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R126) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L129) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G130) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E133) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A134) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (= F137) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P138) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I141) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ F144) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ ATIDR 231:235) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P302) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
371 G→V: Remains in the endoplasmic reticulum.

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
22% identity, 58% coverage: 57:318/455 of query aligns to 128:394/570 of Q9C0U9

query
sites
Q9C0U9

L

L

E

F

S

D

D

D

L

V

G

H

I

I

S

T

A

S

A

N

A

Q

Y

Y

G

N

F

N

G

L

A

N

G

T

L

L

F

F

F

Y

I

V

G

G

Y

Y

A

I

L

V

F

G

E

Q

V

F

P

P

S

G

N

H

M

Y

L

I

L

M

Q

Q

K

T

V

F

G

P

A

L

R

G

L

K

W

F

L

V

T

G

R

L

I

V

M

T

F

F

T

S

W

W

G

S

L

V

V

I

A

V

T

F

A

L

M

H

A

C

F

C

V

A

Q

Y

N

N

E

Y

T

G

Q

G

F

L

Y

I

V

A

L

L

R

R

F

F

L

F

L

L

G

G

V

F

A

T

E

E

A

S

G

C

F

L

P

P

G

A

V

M

I

E

Y

A

Y

T

F

M

T

G

R

M

W

F

L

F

P

T

A

H

A

Q

E

E

R

Q

G

A

K

F

A

L

I

Q

A

P

I

V

F

F

L

W

S

I

G

S

S

C

A

L

L

S

A

C

S

G

L

I

I

P

S

A

G

G

P

F

L

I

A

A

G

Y

A

G

L

L

M

-

Q

E

I

F

Q

V

G

T

L

K

G

S

L

I

H

A

G

P

W

W

Q

K

W

L

M

F

L

M

F

I

I

I

E

T

G

G

M

G

A

I

S

T

V

F

A

F

L

L

C

S

F

I

F

F

V

L

F

F

W

Y

L

Y

D

P

S

D

K

N

P

P

Q

S

D

K

A

A

K

R

W

F

L

L

S

T

K

D

A

E

E

E

Q

K

D

-

A

-

L

-

V

L

A

Y

T

T

I

I

D

D

R

R

E

V

Q

R

Q

K

A

S

R

T

E

R

A

-

I

-

G

G

A

G

V

I

R

E

P

N

S

K

A

I

W

F

N

K

L

K

-

H

-

Q

-

F

-

I

-

E

-

A

L

L

K

K

D

D

R

P

Q

I

I

T

V

W

L

L

F

F

C

T

L

F

I

A

Y

A

F

F

C

T

I

L

Q

M

L

L

T

S

-

N

-

L

I

A

Y

Y

A

Q

T

N

F

L

W

I

L

F

P

T

S

S

I

L

I

-

K

-

R

N

M

V

G

S

D

D

L

L

S

N

D

S

M

T

Q

L

V

V

G

G

F

V

-

A

-

L

-

A

-

G

F

Y

N

N

S

T

I

V

P

S

W

A

L

I

I

I

S

A

I

T

L

F

A

A

M

M

Y

Y

A

L

F

I

A

P

A

N

G

Q

S

S

A

A

Sites not aligning to the query:

14 modified: Phosphoserine

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 74% coverage: 73:408/455 of query aligns to 129:470/582 of Q9JI12

query
sites
Q9JI12

G

G

L

S

F

F

I

W

G

G

Y

Y

A

I

L

I

F

T

E

Q

V

I

P

P

S

G

N

G

M

Y

L

I

L

A

Q

S

K

R

V

L

G

A

A

A

-

N

R

R

L

V

W

F

L

G

T

A

R

A

I

I

M

L

F

L

T

T

-

S

-

T

W

L

G

N

L

M

V

L

A

I

T

P

A

S

M

A

A

A

F

R

V

V

Q

H

N

Y

E

G

T

C

Q

V

F

I

Y

F

V

V

L

-

R
|
R

F

I

L

L

L

Q

G

G

V

L

A

V

E
|
E

A

G

G

V

F

T

F

Y

P

P

G

A

V

C

I

H

Y

G

Y

I

F

W

T

S

R

K

W

W

L

A

P

P

A

P

A

L

E

E

R

R

G

S

K

R

A

L

I

A

A

T

I

T

F

S

L

F

S

C

G

G

S

S

A

Y

L

A

A

G

S

A

L

V

I

I

S

A

G

M

P

P

L

L

A

A

G

G

A

I

L

L

M

V

Q

Q

I

Y

Q

T

G

G

L

-

G

-

L

-

H

-

G

-

W

W

Q

S

W

S

M

V

L

F

F

Y

I

V

E

Y

G

G

M

-

A

-

S

S

V

F

A

G

L

M

C

V

F

W

F

Y

V

M

F

F

F

W

W

L

L

L

D

V

S

S

K

Y

P

E

Q

S

D

P

A

A

K

K

W

H

-

P

-

T

L

I

S

T

K

D

A

E

E

E

Q

R

D

R

A

Y

L

I

V

E

A

E

T

S

I

I

D

G

R

E

E

S

Q

A

Q

N

A

L

R

L

E

G

A

A

I

M

G

E

A

K

V

F

R

K

P

T

S

P

A

W

W

R

N

K

L

F

K

T

D

S

R

M

Q

P

I

V

V

Y

L

A

F

I

C

I

L

V

I

A

Y

N

F

F

C

C

I
x
R

Q

S

L

W

T

T

I

F

Y

Y

A

L

T

L

F

I

W

S

L

Q

P

P

S

A

I

Y

I

F

K

E

R

E

M

V

G

F

D

G

L

F

S

E

D

I

M

S

Q

K

V

V

G

G

F

M

F

L

N

S

S

A

I

V

P

P

W

H

L

L

I

V

S

M

-

T

-

I

I

I

L

V

A

P

M

I

Y

G

A

G

F

Q

A

I

A

A

G

D

S

F

A

L

R

R

W

S

K

K

-

Q

-

I

-

L

-

S

-

T

F

V

Q

R

Q

K

A

I

W

M

V

N

A

C

G

G

A

G

L

F

L

G

V

M

A

E

A

A

T

T

G

L

M

L

F

L

M

V

-

V

-

G

-

Y

S

S

T

H

T

T

G

R

G

G

P

V

V

A

F

I

A

S

F

F

V

L

A

V

V

L

C

-

F

-

A

-

A

A

I

V

G

G

F

F

K

S

S

G

A

F

S

A

S

I

L

S

F

G

W

F

P

N

I

V

P

N

Q

H

G

L

Y

D

L

I

D

A

A

P

R

R

I

Y

A

A

A

S

A

I

V

L

I

M

A

G

L

I

I

S

N

N

S

G

V

V

G

G

N

T

L

L

G

S

G

G

F

M

V

V

A

C

P

P

T

I

F

V

G

G

L

A

L

M

E

T

Q

K

Q

N

R184 (= R126) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E133) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ I269) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
128 H→A: Greatly lowers L-glutamate transport.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
25% identity, 57% coverage: 30:288/455 of query aligns to 29:294/448 of Q51955

query
sites
Q51955

R

R

H

V

V

V

L

L

P

L

L

C

F

F

V

L

I

I

M

V

F

F

I

L

V

-

N
x
D

Y

G

L

L

D
|
D

R

T

V

A

N

A

I

M

G

G

F

F

V

I

R

A

P

P

H

A

L

L

E

S

S

Q

D

E

L

W

G

G

I

I

S

D

A

R

A

A

A

S

Y

L

G

G

F

P

G

V

A

M

G

S

L

A

F

A

F

L

I

I

G

G

Y

M

A

V

L

F

F

G

E

A

V

L

P

G

S

S

N
x
G

M

P

L

L

L

A

Q
x
D

K

R

V

F

G
|
G

A

R

R

K

L

G

W

V

L

L

T

V

R

G

I

A

M

V

F

L

T

V

W

F

G

G

L

G

V

F

A

S

T

L

A

A

M

S

A

A

F

Y

V

A

Q

T

N

N

E

V

T

D

Q

Q

F

L

Y

L

V

V

L

L

R
|
R

F

F

L

L

L

T

G

G

V

L

A

G

E

L

A

G

G

A

F

G

F

M

P

P

G

N

V

A

I

T

Y

T

Y

L

F

L

T

S

R
x
E

W

Y

L

T

P

P

A

-

E

E

R

R

G

L

K

K

A

S

I

L

A

-

I

-

F

-

L

L

S

V

G

T

S

S

A

M

L

F

A

C

S

G

L

F

I

N

S

L

G

G

P

M

L

A

A

G

G

G

A

G

L

F

M

I

Q

S

I

A

Q

K

G

M

L

I

G

P

L

A

H

Y

G

G

W

W

Q
x
H

W

S

M

L

L

L

F

V

I

I

E

G

G

G

M

V

A

L

S

P

V

L

A

L

L

L

C

A

F

L

F

V

V

L

-

M

-

V

-

W

-

L

-

P

-

E

-

S

-

A

-

R

F

F

F

L

W

V

L

V

D

R

S

N

K

R

P

G

Q

T

D

D

A

K

-

I

-

R

K

K

W

T

L

L

S

S

K

P

-

I

-

A

-

P

-

Q

-

V

A

A

E

E

Q

A

D

G

A

S

L

F

V

S

A

V

T

P

I

E

D

Q

R

K

E

A

Q

V

Q

A

A

A

R

R

E

S

A

V

I

F

G

A

A

V

V

I

R

F

P

S

S

G

A

T

W

Y

N

G

L

L

L

-

K

-

D

-

R

-

Q

G

I

T

V

M

L

L

F

L

C

W

L

L

I

T

Y

Y

F

F

C

M

I

G

Q

L

L

V

T

I

I

V

Y

Y

A

L

T

T

F

S

W

W

L

L

P

P

S

T

I

L

I

M

K

R

R

D

M

S

G

G

D41 (≠ N43) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
D44 (= D46) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
G85 (≠ N87) mutation to V: Abolishes 4-HBA transport and chemotaxis.
D89 (≠ Q91) mutation to N: Abolishes 4-HBA transport and chemotaxis.
G92 (= G94) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
R124 (= R126) mutation to A: Abolishes 4-HBA transport.
E144 (≠ R146) mutation to A: Strong decrease in 4-HBA transport.
H183 (≠ Q190) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

323 D→N: Abolishes 4-HBA transport and chemotaxis.
328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
386 R→A: Strong decrease in 4-HBA transport.
398 R→A: Abolishes 4-HBA transport.
444 H→A: No change in 4-HBA transport and chemotaxis.

A0A0H2VG78 Glucose transporter GlcP; Glucose/H(+) symporter from Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) (see paper)
24% identity, 51% coverage: 57:288/455 of query aligns to 33:270/446 of A0A0H2VG78

query
sites
A0A0H2VG78

L

I

E

H

S

K

D

D

L

I

G

P

I

L

S

N

A

S

A

T

Y

E

G

G

F

I

G

V

A

V

G

S

L

S

F

M

F

L

I

I

G

G

Y

A

A

I

L

V

F

G

E

A

V

G

P

S

S

S

N

G

M

P

L

L

L

A

Q

D

K

K

V

L

G

G

A

R

R

R

L

R

W

L

L

V

T

M

R

L

I

I

M

A

F

I

T

V

W

F

G

I

L

I

V

G

A

A

T

L

A

I

M

L

A

A

F

A

V

S

Q

T

N

N

E

L

T

A

Q

L

F

L

Y

I

V

I

L

G

R
|
R

F

L

L

I

L
x
I

G

G

V

L

A

A

E

V

A

G

G

G

F

S

F

M

P

S

G

T

V

V

I

P

Y

V

Y

Y

F

L

T

S

R
x
E

W

M

L

A

P

P

A

T

A

E

E

Y

R

R

G

G

K

S

A

L

I

-

A

-

I

-

F

-

L

-

S

-

G

G

S

S

A

L

L

N

A
x
Q

S

L

L

M

I

I

S

T

-

I

G

G

P

I

L

L

A

A

G

A

A

Y

L

L

M

V

Q

N

I

Y

Q

A

G

F

L

A

G

D

L

I

H

E

G

G

W

W

Q

R

W

W

M

M

L

L

F

G

I

L

E

A

G

V

M

V

A

P

S

S

V

V

A

I

L

L

C

L

F

V

F

G

V

I

F

Y

F

F

W

-

L

-

D

-

S

-

K

M

P

P

Q

E

D

S

A

P

K

R

W

W

L

L

-

L

-

E

-

N

-

R

-

N

S

E

K

E

A

A

E

A

Q

R

D

Q

A

V

L

M

V

K

A

I

T

T

-

Y

-

D

-

S

-

E

I

I

D

D

R

K

E

E

Q

L

Q

K

A

E

R

M

E

K

A

E

I

I

G

N

A

A

V

I

R

S

P

E

S

S

A

T

W

W

N

T

L

V

L

I

K

K

D

S

-

P

-

W

-

L

-

G

R

R

Q

I

I

L

V

I

L

V

F

G

C

C

L

I

I

F

Y

A

F

I

C

F

I
x
Q

Q
|
Q

-

F

L

I

T

G

I

I

Y
x
N

A

A

A

V

T

I

F

F

W

Y

L

S

P

S

S

S

I

I

I

F

K

A

R

K

M

A

G

G

R102 (= R126) mutation to A: Loss of transport activity.
I105 (≠ L129) mutation to S: Affects symport activity. May function as an uniporter.
E122 (≠ R146) mutation to A: Loss of transport activity.
Q137 (≠ A167) mutation to A: Loss of transport activity.
Q250 (≠ I269) mutation to A: Loss of transport activity.
Q251 (= Q270) mutation to A: Loss of transport activity.
N256 (≠ Y274) mutation to A: Loss of transport activity.

Sites not aligning to the query:

22 D→N: Affects symport activity. May function as an uniporter.
357 W→A: Loss of transport activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
23% identity, 58% coverage: 26:288/455 of query aligns to 18:285/452 of Q5EXK5

query
sites
Q5EXK5

A

A

K

R

V

Q

K

W

R

R

H

V

V

I

L

I

P

C

L

C

F

F

V

L

I

V

M

V

F

M

I

L

V

D

N

G

Y

F

L

-

D

D

R

T

V

A

N

A

I

I

G

G

F

F

V

I

R

A

P

P

H

D

L

I

E

R

S

S

D

H

L

W

G

Q

I

L

S

T

A

A

A

G

A

D

Y

L

G

A

F

P

G

L

A

F

G

G

L

A

F

G

F

L

I

L

G

G

Y

L

A

T

L

A

F

G

E

A

V

L

P

L

S

C

N

G

M

P

L

L

L

S

Q
x
D

K

R

V

F

G

G

A

R

R

K

L

R

W

V

L

I

T

E

R

F

I

C

M

V

F

A

T

L

W

F

G

G

L

L

V

F

A

S

T

L

A

L

M

S

A

A

F

F

V

A

Q

P

N

D

E

L

T

Q

Q

M

F

L

Y

V

V

F

L

L

R

R

F

F

L

L

L

T

G

G

V

L

A

G

E

L

A

G

G

G

F

A

F

M

P

P

G

N

V

T

I

I

Y

T

Y

M

F

T

T

S

R

E

W

Y

L

L

P

P

A

A

A

R

E

R

R

R

G

G

K

A

A

L

I

V

A

T

I

L

F

M

L

F

S

C

G

G

S

F

A

T

L

L

A

G

S

S

L

A

I

F

S

G

G

G

P

V

L

V

A

S

G

A

A

Q

L

L

M

V

Q

P

I

-

Q

-

G

-

L

-

G

-

L

L

H

I

G

G

W

W

Q

H

W

G

M

I

L

L

F

V

I

L

E

G

G

G

M

V

A

L

S

P

V

L

A

I

L

L

C

A

F

V

F

A

V

L

F

V

F

P

W

L

L

L

D

P

S

E

K

S

P

P

-

R

-

W

Q

Q

D

I

A

R

K

R

W

Q

L

L

S

P

K

Q

A

A

E

T

Q

I

D

A

A

R

L

T

V

V

A

G

T

A

I

I

D

T

R

G

E

E

Q

R

Q

Y