SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_4998 FitnessBrowser__Putida:PP_4998 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6pnzA The structure of the aspartate transcarbamoylase trimer from staphylococcus aureus complexed with pala at 2.27 resolution.
39% identity, 89% coverage: 18:315/334 of query aligns to 1:288/293 of 6pnzA

query
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6pnzA

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binding n-(phosphonacetyl)-l-aspartic acid: S48 (= S69), T49 (= T70), R50 (= R71), T51 (= T72), S75 (= S96), K78 (= K99), R100 (= R121), H127 (= H151), R160 (= R184), R210 (= R239), Q212 (= Q241), A253 (≠ G280)

4bjhB Crystal structure of the aquifex reactor complex formed by dihydroorotase (h180a, h232a) with dihydroorotate and aspartate transcarbamoylase with n-(phosphonacetyl)-l-aspartate (pala) (see paper)
40% identity, 91% coverage: 18:320/334 of query aligns to 1:291/291 of 4bjhB

query
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4bjhB

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active site: R47 (= R71), T48 (= T72), K75 (= K99), R97 (= R121), H126 (= H151), Q129 (= Q154)
binding n-(phosphonacetyl)-l-aspartic acid: S45 (= S69), T46 (= T70), R47 (= R71), T48 (= T72), R97 (= R121), H126 (= H151), R159 (= R184), V160 (= V185), R213 (= R239), Q215 (= Q241), G251 (= G280)

3d6nB Crystal structure of aquifex dihydroorotase activated by aspartate transcarbamoylase (see paper)
40% identity, 91% coverage: 18:320/334 of query aligns to 1:291/291 of 3d6nB

query
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3d6nB

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active site: R47 (= R71), T48 (= T72), K75 (= K99), R97 (= R121), H126 (= H151), Q129 (= Q154)
binding citrate anion: T48 (= T72), R97 (= R121), H126 (= H151), R159 (= R184), V160 (= V185), R213 (= R239), G251 (= G280)

3r7fA Crystal structure of cp-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
37% identity, 90% coverage: 18:319/334 of query aligns to 1:289/291 of 3r7fA

query
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3r7fA

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L

active site: R49 (= R71), T50 (= T72), K77 (= K99), R99 (= R121), H127 (= H151), Q130 (= Q154), L210 (= L238), P249 (= P279), G277 (= G307)
binding phosphoric acid mono(formamide)ester: S47 (= S69), T48 (= T70), R49 (= R71), T50 (= T72), R99 (= R121), H127 (= H151), Q130 (= Q154), P249 (= P279), A250 (≠ G280)
binding phosphate ion: S11 (≠ P28), T12 (≠ R29), Q23 (≠ D40), K26 (≠ R48), E140 (≠ R164), R171 (≠ A195), K241 (= K271), H243 (≠ D273), K272 (≠ N302), K272 (≠ N302), K275 (≠ T305)

3r7dA Crystal structure of unliganded aspartate transcarbamoylase from bacillus subtilis (see paper)
37% identity, 90% coverage: 18:319/334 of query aligns to 1:289/291 of 3r7dA

query
sites
3r7dA

L

M

R

K

H

H

F

L

L

T

S

T

L

M

D

S

G

E

L

L

P
x
S

R
x
T

E

E

L

E

L

I

T

K

E

D

I

L

L

L

D

Q

T

T

A

A

D

Q

S

E

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

S

V

G

K

K

K

T

V

D

P

N

L

Q

L

L

R

T

G

G

K

K

T

F

V

A

C

A

N

N

V

L

F

F

E

E

N

P

S

S

T

T

R
|
R

T
|
T

R

R

T

F

T

S

F

F

E

E

L

V

A

A

A

E

Q

K

R

K

L

L

S

G

A

M

D

N

V

V

I

L

S

N

L

L

N

D

V

G

S

T

T

S

S
x
T

S
|
S

T

V

S

Q

K
|
K

G

G

E

E

T

T

L

L

F

Y

D

D

T

T

L

I

R

R

N

T

L

L

E

E

A

S

M

I

A

G

A

V

D

D

M

V

F

C

V

V

V

I

R
|
R

H

H

S

S

D

E

S

D

G

E

A

Y

H

E

F

E

I

L

A

V

E

S

H

Q

V

V

C

-

P

-

E

N

V

I

A

P

V

I

I

L

N

N

G

A

G

G

D

D

G

G

R

C

H

G

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

S

M

L

L

L

D

D

M

L

L

M

T

T

I

I

R

Y

R

E

H

E

K

F

G

N

S

T

F

F

E

K

N

G

L

L

S

T

V

V

A

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

R

R

S

S

D

N

M

A

L

E

A

V

L

L

K

T

A
x
R

L

L

G

G

C

A

P

-

D

-

I

-

R

R

V

V

I

L

-

F

-

S

G

G

P

P

K

S

T

E

L

W

I

Q

P

D

I

-

G

-

I

-

E

E

Q

E

Y

N

G

T

V

F

K

G

V

T

Y

Y

T

V

D

S

L

M

A

D

E

E

G

A

L

V

K

E

D

S

V

S

D

D

V

V

I

M

M

L

L
|
L

R

R

L

I

Q

Q

R

N

E

E

R

R

M

H

A

Q

G

S

G

A

L

V

L

-

P

-

S

S

E

Q

G

E

E

G

F

Y

Y

L

R

N

L

K

F

Y

G

G

L

L

T

T

T

V

A

E

R

R

L

A

A

E

G

R

A

M

K

K

P

R

D

H

A

A

I

I

V

I

M

M

H

H

P
|
P

G

A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

E

D

S

D

A

S

V

L

A

V

D

E

G

S

K

E

H

K

S

S

V

R

I

I

L

F

N

K

Q

Q

V

M

T

K

Y

N

G
|
G

I

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

S

Q

M

R

A

A

M

L

active site: R49 (= R71), T50 (= T72), K77 (= K99), R99 (= R121), H127 (= H151), Q130 (= Q154), L210 (= L238), P249 (= P279), G277 (= G307)
binding phosphate ion: S11 (≠ P28), T12 (≠ R29), T73 (≠ S95), S74 (= S96), K77 (= K99), R171 (≠ A195)

3r7lA Crystal structure of pala-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
37% identity, 90% coverage: 18:319/334 of query aligns to 1:289/290 of 3r7lA

query
sites
3r7lA

L

M

R

K

H

H

F

L

L

T

S

T

L

M

D

S

G

E

L

L

P

S

R

T

E

E

L

E

L

I

T

K

E

D

I

L

L

L

D

Q

T

T

A

A

D

Q

S

E

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

S

V

G

K

K

K

T

V

D

P

N

L

Q

L

L

R

T

G

G

K

K

T

F

V

A

C

A

N

N

V

L

F

F

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

F

T

S

F

F

E

E

L

V

A

A

A

E

Q

K

R

K

L

L

S

G

A

M

D

N

V

V

I

L

S

N

L

L

N

D

V

G

S

T

T

S

S

T

S
|
S

T

V

S

Q

K
|
K

G

G

E

E

T

T

L

L

F

Y

D

D

T

T

L

I

R

R

N

T

L

L

E

E

A

S

M

I

A

G

A

V

D

D

M

V

F

C

V

V

V

I

R
|
R

H

H

S

S

D

E

S

D

G

E

A

Y

H

E

F

E

I

L

A

V

E

S

H

Q

V

V

C

-

P

-

E

N

V

I

A

P

V

I

I

L

N

N

G

A

G

G

D

D

G

G

R

C

H

G

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

S

M

L

L

L

D

D

M

L

L

M

T

T

I

I

R

Y

R

E

H

E

K

F

G

N

S

T

F

F

E

K

N

G

L

L

S

T

V

V

A

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R
|
R

V

V

A

A

R

R

S

S

D

N

M

A

L

E

A

V

L

L

K

T

A

R

L

L

G

G

C

A

P

-

D

-

I

-

R

R

V

V

I

L

-

F

-

S

G

G

P

P

K

S

T

E

L

W

I

Q

P

D

I

-

G

-

I

-

E

E

Q

E

Y

N

G

T

V

F

K

G

V

T

Y

Y

T

V

D

S

L

M

A

D

E

E

G

A

L

V

K

E

D

S

V

S

D

D

V

V

I

M

M

L

L
|
L

R
|
R

L

I

Q
|
Q

R

N

E

E

R

R

M

H

A

Q

G

S

G

A

L

V

L

-

P

-

S

S

E

Q

G

E

E

G

F

Y

Y

L

R

N

L

K

F

Y

G

G

L

L

T

T

T

V

A

E

R

R

L

A

A

E

G

R

A

M

K

K

P

R

D

H

A

A

I

I

V

I

M

M

H

H

P
|
P

G
x
A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

E

D

S

D

A

S

V

L

A

V

D

E

G

S

K

E

H

K

S

S

V

R

I

I

L

F

N

K

Q

Q

V

M

T

K

Y

N

G
|
G

I

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

S

Q

M

R

A

A

M

L

active site: R49 (= R71), T50 (= T72), K77 (= K99), R99 (= R121), H127 (= H151), Q130 (= Q154), L210 (= L238), P249 (= P279), G277 (= G307)
binding n-(phosphonacetyl)-l-aspartic acid: S47 (= S69), T48 (= T70), R49 (= R71), T50 (= T72), S74 (= S96), K77 (= K99), R99 (= R121), H127 (= H151), R160 (= R184), R211 (= R239), Q213 (= Q241), A250 (≠ G280)

P05654 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Bacillus subtilis (strain 168) (see paper)
37% identity, 90% coverage: 18:319/334 of query aligns to 1:289/304 of P05654

query
sites
P05654

L

M

R

K

H

H

F

L

L

T

S

T

L

M

D

S

G

E

L

L

P

S

R

T

E

E

L

E

L

I

T

K

E

D

I

L

L

L

D

Q

T

T

A

A

D

Q

S

E

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

S

V

G

K

K

K

T

V

D

P

N

L

Q

L

L

R

T

G

G

K

K

T

F

V

A

C

A

N

N

V

L

F

F

E

E

N

P

S

S

T

T

R

R

T

T

R

R

T

F

T

S

F

F

E

E

L

V

A

A

A

E

Q

K

R

K

L

L

S

G

A

M

D

N

V

V

I

L

S

N

L

L

N

D

V

G

S

T

T

S

S

T

S

S

T

V

S

Q

K

K

G

G

E

E

T

T

L

L

F

Y

D

D

T

T

L

I

R

R

N

T

L

L

E

E

A

S

M

I

A

G

A

V

D

D

M

V

F

C

V

V

V

I

R

R

H

H

S

S

D

E

S

D

G

E

A

Y

H

E

F

E

I

L

A

V

E

S

H

Q

V

V

C

-

P

-

E

N

V

I

A

P

V

I

I

L

N

N

G

A

G

G

D

D

G

G

R

C

H

G

A

Q

H

H

P

P

T

T

Q

Q

G

S

M

L

L

L

D

D

M

L

L

M

T

T

I

I

R

Y

R

E

H

E

K

F

G

N

S

T

F

F

E

K

N

G

L

L

S

T

V

V

A

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

R

R

S

S

D

N

M

A

L

E

A

V

L

L

K

T

A

R

L

L

G

G

C

A

P

-

D

-

I

-

R

R

V

V

I

L

-

F

-

S

G

G

P

P

K

S

T

E

L

W

I

Q

P

D

I

-

G

-

I

-

E

E

Q

E

Y

N

G

T

V

F

K

G

V

T

Y

Y

T

V

D

S

L

M

A

D

E

E

G

A

L

V

K

E

D

S

V

S

D

D

V

V

I

M

M

L

L

L

R

R

L

I

Q

Q

R

N

E

E

R

R

M

H

A

Q

G

S

G

A

L

V

L

-

P

-

S

S

E

Q

G

E

E

G

F

Y

Y

L

R

N

L

K

F

Y

G

G

L

L

T

T

T

V

A

E

R

R

L

A

A

E

G

R

A

M

K

K

P

R

D

H

A

A

I

I

V

I

M

M

H

H

P

P

G

A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

E

D

S

D

A

S

V

L

A

V

D

E

G

S

K

E

H

K

S

S

V

R

I

I

L

F

N

K

Q

Q

V

M

T

K

Y

N

G

G

I

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

S

Q

M

R

A

A

M

L

Sites not aligning to the query:

303 modified: Phosphoserine

8bplA Aspartate transcarbamoylase mutant (n2045c, r2238c) from chaetomium thermophilum cad-like bound to carbamoyl phosphate (see paper)
36% identity, 90% coverage: 20:320/334 of query aligns to 17:316/316 of 8bplA

query
sites
8bplA

H

H

F

V

L

L

S

S

L

V

D

T

G

Q

L

F

P

T

R

R

E

A

L

D

L

L

T

H

E

L

I

L

L

F

D

Q

T

I

A

A

D

Q

S

E

F

-

L

M

E

R

V

L

G

G

A

V

R

Q

A

R

V

E

K

G

K

V

V

L

P

D

L

I

L

L

R

R

G

G

K

K

T

V

V

L

C

C

N

T

V

L

F

F

F

Y

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

R

S

T

A

T

S

F

F

E

D

L

A

A

A

A

M

Q

Q

R

R

L

L

S

G

A

G

D

R

V

T

I

I

S

P

L

I

N

Q

V

T

S

S

T

T

S

S

T

V

S

Q

K

K

G

G

E

E

T

T

L

L

F

Q

D

D

T

T

L

L

R

R

N

T

L

L

E

-

A

A

M

C

A

Y

A

S

D

D

M

A

F

I

V

V

V

L

R
|
R

H

H

S

P

D

D

S

E

G

K

A

C

A

V

H

D

F

-

I

V

A

A

E

K

H

K

V

Y

C

C

P

P

E

-

V

V

A

P

V

V

I

I

N

N

G

G

D

N

G

G

R

S

H

K

A

E

H
|
H

P

P

T

T

Q
|
Q

G

A

M

F

L

L

D

D

M

L

L

F

T

T

I

I

R

R

R

E

H

E

K

L

G

G

S

T

F

M

E

Q

N

G

L

L

S

T

V

I

A

T

I

F

V

V

G

G

D

D

I

L

L

L

H

Y

S

G

R

R

V

P

A

V

R

H

S

S

D

L

M

V

L

Y

A

L

L

L

K

R

A

H

L

Y

G

Q

C

V

P

-

D

K

I

V

R

Q

V

L

I

V

G

S

P

P

K

K

T

A

L

L

-

R

I

L

P

P

I

P

G

A

I

V

E

R

Q

Q

Y

Q

G

L

V

V

K

D

V

A

Y

G

T

Q

D

L

L

L

A

C

E

E

G

S

-

E

-

A

-

L

-

T

-

P

-

E

-

I

L

L

K

G

D

R

V

T

D

D

V

V

V

L

I

Y

M

C

L

T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

-

L

F

P

P

S

S

E

L

G

A

E

E

F

F

Y

E

R

A

L

V

-

K

-

D

-

S

F

Y

G

R

L

I

T

D

T

Y

A

S

R

T

L

L

A

K

G

Y

A

A

K

K

P

P

D

T

A

T

I

V

V

V

M

M

H

H

P

P

G
x
L

P

P

I

R

N

N

R

E

G

-

V

-

E

E

I

V

E

A

S

E

A

E

V

V

A

D

D

F

G

D

K

Q

H

R

S

A

V

A

I

Y

L

F

N

R

Q

Q

V

M

T

C

Y

Y

G

G

I

L

A

Y

V

C

R

R

M

M

A

A

V

L

L

L

S

A

M

L

A

V

M

M

S

S

binding phosphoric acid mono(formamide)ester: S65 (= S69), T66 (= T70), R67 (= R71), T68 (= T72), R116 (= R121), H144 (= H151), Q147 (= Q154), L278 (≠ G280)

1ml4A The pala-liganded aspartate transcarbamoylase catalytic subunit from pyrococcus abyssi (see paper)
35% identity, 90% coverage: 19:319/334 of query aligns to 5:304/307 of 1ml4A

query
sites
1ml4A

R

R

H

D

F

V

L

I

S

S

L

I

D

R

G

D

L

F

P

S

R

K

E

E

L

D

L

I

T

E

E

T

I

V

L

L

D

A

T

T

A

A

D

E

S

R

F

-

L

L

E

E

V

R

G

E

A

L

R

K

A

E

V

K

K

G

K

Q

V

L

P

E

L

Y

L

A

R

K

G

G

K

K

T

I

V

L

C

A

N

T

V

L

F

F

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

S

A

A

A

M

Q

H

R

R

L

L

S

G

A

G

D

A

V

V

I

I

S

G

L

F

-

A

N

E

V

A

S

S

T

T

S

S

T

V

S

K

K
|
K

G

G

E

E

T

S

L

L

F

R

D

D

T

T

L

I

R

K

N

T

L

V

E

E

A

Q

M

Y

A

C

A

-

D

D

M

V

F

I

V

V

V

I

R
|
R

H

H

S

P

D

K

S

E

G

G

A

A

H

R

F

L

I

A

A

A

E

E

H

-

V

-

C

V

P

A

E

E

V

V

A

P

V

V

I

I

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

Y

T

T

I

I

R

K

H

E

K

F

G

G

S

R

F

I

E

D

N

G

L

L

S

K

V

I

A

G

I

L

V

L

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

D

L

M

A

L

E

A

A

L

L

K

T

A

F

L

Y

G

D

C

V

P

-

D

E

I

L

R

Y

V

L

I

I

G

S

P

P

K

E

T

L

L

L

-

R

-

M

-

P

-

R

-

H

I

I

P

V

I

E

G

E

I

L

E

R

Q

E

Y

K

G

G

V

M

K

K

V

V

Y

V

-

E

-

T

T

T

D

T

L

L

A

E

E

D

G

V

L

I

K

G

D

K

V

L

D

D

V

V

V

L

I

Y

M

V

L
x
T

R
|
R

L

I

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

-

L

F

P

P

S

D

E

E

G

Q

E

E

F

Y

Y

L

R

K

L

V

F

K

G

G

-

S

-

Y

-

Q

L

V

T

N

T

L

A

K

R

V

L

L

A

E

G

K

A

A

K

K

P

D

D

E

A

L

I

R

V

I

M

M

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

H

S

P

A

E

V

V

A

D

D

N

G

T

K

K

H

H

S

A

V

I

I

Y

L

F

N

R

Q

Q

V

V

T

F

Y

N

G
|
G

I

V

A

P

V

V

R

R

M

M

A

A

V

L

L

L

S

A

M

L

A

V

M

L

active site: R56 (= R71), T57 (= T72), K85 (= K99), R106 (= R121), H134 (= H151), Q137 (= Q154), T227 (≠ L238), P266 (= P279), G292 (= G307)
binding n-(phosphonacetyl)-l-aspartic acid: S54 (= S69), T55 (= T70), R56 (= R71), T57 (= T72), R106 (= R121), H134 (= H151), R167 (= R184), T168 (≠ V185), R228 (= R239), L267 (≠ G280)

4eknB Structure of the catalytic chain of methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form (see paper)
35% identity, 90% coverage: 18:316/334 of query aligns to 1:297/304 of 4eknB

query
sites
4eknB

L

M

R

K

H

H

F

L

L

I

S

S

L

M

D

K

G

D

L

I

P

G

R

K

E

E

L

E

L

I

T

L

E

E

I

I

L

L

D

D

T

E

A

A

D

R

S

K

F

M

L

E

E

E

V

L

G

-

A

L

R

N

A

T

V

K

K

R

K

P

V

L

P

K

L

L

R

E

G

G

K

K

T

I

V

L

C

A

N

T

V

V

F

F

F

Y

E

E

N

P

S

S

T

T

R

R

T

T

R

R

T

L

T

S

F

F

E

E

L

T

A

A

A

M

Q

K

R

R

L

L

S

G

A

G

D

E

V

V

I

I

S

T

L

M

-

T

N

D

V

L

S

K

T

S

S

S

T

V

S

A

K

K

G

G

E

E

T

S

L

L

F

I

D

D

T

T

L

I

R

R

N

V

L

I

E

S

A

G

M

Y

A

A

A

-

D

D

M

I

F

I

V

V

V

L

R

R

H

H

S

P

D

S

S

E

G

G

A

A

H

R

F

L

I

A

A

S

E

E

H

Y

V

-

C

-

P

S

E

Q

V

V

A

P

V

I

I

I

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H

H

P

P

T

T

Q

Q

G

T

M

L

L

L

D

D

M

L

L

Y

T

T

I

I

R

M

R

R

H

E

K

I

G

G

S

R

F

I

E

D

N

G

L

I

S

K

V

I

A

A

I

F

V

V

G

G

D

D

I

L

L

K

H

Y

S
x
G

R
|
R

V
x
T

A

V

R

H

S

S

D

L

M

V

L

Y

A

A

L

L

K

S

A

L

L

F

G

E

C

N

P

V

D

E

I

M

R

Y

V

F

I

V

G

S

P

P

K

K

T

E

L

L

-

R

I

L

P

P

I

K

G

D

I

I

-

I

-

E

-

D

-

L

E

K

Q

A

Y

K

G

N

V

I

K

K

V

F

Y

Y

-

E

-

K

T

E

D

S

L

L

A

D

E

D

G

L

L

D

K

D

D

D

V

I

D

D

V

V

V

L

I

Y

M

V

L
x
T

R
|
R

L

I

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

-

L

F

P

P

S

D

E

P

G

N

E

E

F

Y

Y

E

R

K

L

V

-

K

-

G

-

S

F

Y

G

K

L

I

T

K

T

R

A

E

R

Y

L

V

A

E

G

G

A

K

K

K

P

-

D

-

A

F

I

I

V

I

M

M

H

H

P
|
P

G

L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

D

S

Y

A

D

V

V

A

D

D

D

G

L

K

P

H

Q

S

A

V

K

I

Y

L

F

N

K

Q

Q

V

S

T

F

Y

Y

G
|
G

I

I

A

P

V

V

R

R

M

M

A

A

V

I

L

L

S

K

active site: T225 (≠ L238), P262 (= P279), G288 (= G307)
binding sulfate ion: G163 (≠ S183), R164 (= R184), T165 (≠ V185), R226 (= R239)

2hseA Structure of d236a e. Coli aspartate transcarbamoylase in the presence of phosphonoacetamide and l-aspartate at 2.60 a resolution
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2hseA

query
sites
2hseA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T
x
S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q
|
Q

R

K

E

E

R

R

M

L

A

A

G

-

L

-

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P
|
P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V
x
A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R
|
R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding aspartic acid: R54 (= R71), T55 (= T72), S58 (≠ T75), R105 (= R121), H134 (= H151), Q137 (= Q154), R167 (= R184), R229 (= R239), Q231 (= Q241), L267 (≠ G280), P268 (= P281), A289 (≠ V304), R296 (= R311)
binding phosphonoacetamide: S52 (= S69), T53 (= T70), R54 (= R71), T55 (= T72), R105 (= R121), L267 (≠ G280)

2a0fA Structure of d236a mutant e. Coli aspartate transcarbamoylase in presence of phosphonoacetamide at 2.90 a resolution (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2a0fA

query
sites
2a0fA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S

S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V

T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

A

A

G

-

L

-

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R

R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding phosphonoacetamide: R54 (= R71), T55 (= T72), H134 (= H151), Q137 (= Q154), L267 (≠ G280)

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 80% coverage: 53:319/334 of query aligns to 1970:2237/2244 of Q09794

query
sites
Q09794

V

I

P

D

L

L

L

C

R

Y

G

G

K

K

T

L

V

L

C

C

N

T

V

M

F

F

E

E

N

P

S

S

T

T

R

R

T

T

R

S

T

S

F

F

E

D

L

A

A

A

A

M

Q

Q

R

R

L

L

S

G

A

G

D

K

V

V

I

V

S

A

L

V

N

T

V

A

S

S

T

A

S

S

T

V

S

N

K

K

G

G

E

E

T

S

L

L

F

A

D

D

T

T

L

I

R

R

N

T

L

L

E

-

A

G

M

C

A

Y

A

G

D

D

M

A

F

I

V

V

V

L

R

R

H

H

S

P

D

S

S

I

G

E

A

S

A

A

H

R

F

-

I

I

A

A

E

A

H

N

V

F

C

S

P

P

E

-

V

V

A

P

V

I

I

I

N

N

G

G

D

N

G

G

R

S

H

K

A

E

H

H

P

P

T

T

Q

Q

G

A

M

F

L

L

D

D

M

L

L

Y

T

T

I

I

R

R

R

E

H

E

K

L

G

G

S

S

F

V

E

N

N

G

L

L

S

T

V

I

A

T

I

F

V

I

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V

T

A

V

R

H

S

S

D

-

M

L

L

A

A

R

L

L

K

L

A

A

L

F

G

W

C

H

P

V

D

E

I

L

R

H

V

L

I

V

G

S

P

P

K

E

T

Q

L

L

-

A

-

L

-

P

-

D

I

V

P

K

I

D

G

D

I

I

E

R

Q

A

Y

N

G

G

V

L

K

N

V

-

Y

F

T

I

D

E

L

H

A

R

E

E

G

L

L

T

K

K

D

E

V

V

-

V

-

A

-

Q

-

S

D

D

V

V

V

L

I

Y

M

C

L

T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

F

A

A

G

-

L

-

P

-

S

S

E

V

G

D

E

E

F

Y

Y

E

R

K

L

L

-

K

-

D

-

S

F

F

G

I

L

V

T

D

T

N

A

S

R

V

L

L

A

A

G

S

A

A

K

K

P

S

D

H

A

C

I

I

V

V

M

M

H

H

P

P

G

L

P

P

I

R

N

N

R

R

G

E

V

I

E

S

I

-

E

E

S

E

A

V

V

D

A

F

D

D

G

Q

K

R

H

R

S

A

V

A

I

Y

L

F

N

R

Q

Q

V

M

T

R

Y

Y

G

G

I

L

A

Y

V

I

R

R

M

M

A

A

V

L

L

L

S

A

M

C

A

V

M

M

Sites not aligning to the query:

1119 modified: Phosphoserine
1881 modified: Phosphoserine
1885 modified: Phosphoserine

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
32% identity, 90% coverage: 19:319/334 of query aligns to 1924:2222/2225 of P08955

query
sites
P08955

R

Q

H

H

F

I

L

L

S

S

L

V

D

K

G

Q

L

F

P

T

R

K

E

D

L

Q

L

M

T

S

E

H

I

L

L

F

D

N

T

V

A

A

D

H

S

T

F

-

L

L

E

R

V

M

G

M

A

V

R

Q

A

K

V

E

K

R

K

S

V

L

P

D

L

I

L

L

R

K

G

G

K

K

T

V

V

M

C

A

N

S

V

M

F

F

F

Y

E

E

N

V

S

S

T

T

R

R

T

T

R

S

T

S

F

F

E

A

L

A

A

A

A

M

Q

A

R

R

L

L

S

G

A

G

D

A

V

V

I

L

S

S

L

F

N

S

V

E

S

A

T

T

S

S

T

V

S

Q

K

K

G

G

E

E

T

S

L

L

F

A

D

D

T

S

L

V

R

Q

N

T

L

M

E

S

A

C

M

Y

A

-

A

A

D

D

M

V

F

V

V

V

V

L

R

R

H

H

S

P

D

Q

S

P

G

G

A

A

A

V

H

E

F

L

I

A

A

A

E

K

H

H

V

C

C

-

P

-

E

R

V

R

A

P

V

V

I

I

N

N

G

A

G

G

D

D

G

G

R

V

H

G

A

E

H

H

P

P

T

T

Q

Q

G

A

M

L

L

L

D

D

M

I

L

F

T

T

I

I

R

R

R

E

H

E

K

L

G

G

S

T

F

V

E

N

N

G

L

M

S

T

V

I

A

T

I

M

V

V

G

G

D

D

I

L

L

K

H

H

S

G

R

R

V

T

A

V

R

H

S

S

D

L

M

A

L

C

A

L

L

L

K

T

A

Q

L

Y

G

R

C

V

P

-

D

S

I

L

R

R

V

Y

I

V

G

A

P

P

K

P

T

S

L

L

-

R

I

M

P

P

I

P

G

S

I

V

E

W

Q

D

Y

F

-

V

-

A

-

S

-

R

G

G

V

T

K

K

V

Q

-

E

Y

F

T

E

D

S

L

I

A

E

E

E

G

A

L

L

K

P

D

D

V

T

D

D

V

V

V

L

I

Y

M

M

L

T

R

R

L

I

Q

Q

R

K

E

E

R

R

M

F

A

G

G

-

L

-

P

-

S

S

E

T

G

Q

E

E

F

Y

Y

E

R

A

L

C

F

F

G

G

-

Q

-

F

-

I

L

L

T

T

T

P

A

H

R

I

L

M

A

T

G

R

A

A

K

K

P

K

D

K

A

M

I

V

V

V

M

M

H

H

P

P

G

M

P

P

I

-

N

-

R

R

G

V

V

N

E

E

I

I

E

S

S

V

A

E

V

V

A

D

D

S

G

D

K

P

H

R

S

A

V

A

I

Y

L

F

N

R

Q

Q

V

A

T

E

Y

N

G

G

I

M

A

Y

V

I

R

R

M

M

A

A

V

L

L

L

S

A

M

T

A

V

M

L

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
32% identity, 90% coverage: 19:319/334 of query aligns to 1924:2222/2225 of P27708

query
sites
P27708

R
x
Q

H
|
H

F
x
I

L
|
L

S
|
S

L
x
V

D
x
Q

G
x
Q

L
x
F

P
x
T

R
x
K

E
x
D

L
x
Q

L
x
M

T
x
S

E
x
H

I
x
L

L
x
F

D
x
N

T
x
V

A
|
A

D
x
H

S
x
T

F

-

L
|
L

E
x
R

V
x
M

G
x
M

A
x
V

R
x
Q

A
x
K

V
x
E

K
x
R

K
x
S

V
x
L

P
x
D

L
x
I

L
|
L

R
x
K

G
|
G

K
|
K

T
x
V

V
x
M

C
x
A

N
x
S

V
x
M

F
|
F

F
x
Y

E
|
E

N
x
V

S
|
S

T
|
T

R
|
R

T
|
T

R
x
S

T
x
S

F
|
F

E
x
A

L
x
A

A
|
A

A
x
M

Q
x
A

R
|
R

L
|
L

S
x
G

A
x
G

D
x
A

V
|
V

I
x
L

S
|
S

L
x
F

N
x
S

V
x
E

S
x
A

T
|
T

S
|
S

T
x
V

S
x
Q

K
|
K

G
|
G

E
|
E

T
x
S

L
|
L

F
x
A

D
|
D

T
x
S

L
x
V

R
x
Q

N
x
T

L
x
M

E
x
S

A
x
C

M
x
Y

A

-

A
|
A

D
|
D

M
x
V

F
x
V

V
|
V

V
x
L

R
|
R

H
|
H

S
x
P

D
x
Q

S
x
P

G
|
G

A
|
A

A
x
V

H
x
E

F
x
L

I
x
A

A
|
A

E
x
K

H
|
H

V
x
C

C

-

P

-

E
x
R

V
x
R

A
x
P

V
|
V

I
|
I

N
|
N

G
x
A

G
|
G

D
|
D

G
|
G

R
x
V

H
x
G

A
x
E

H
|
H

P
|
P

T
|
T

Q
|
Q

G
x
A

M
x
L

L
|
L

D
|
D

M
x
I

L
x
F

T
|
T

I
|
I

R
|
R

R
x
E

H
x
E

K
x
L

G
|
G

S
x
T

F
x
V

E
x
N

N
x
G

L
x
M

S
x
T

V
x
I

A
x
T

I
x
M

V
|
V

G
|
G

D
|
D

I
x
L

L
x
K

H
|
H

S
x
G

R
|
R

V
x
T

A
x
V

R
x
H

S
|
S

D
x
L

M
x
A

L
x
C

A
x
L

L
|
L

K
x
T

A
x
Q

L
x
Y

G
x
R

C
x
V

P

-

D
x
S

I
x
L

R
|
R

V
x
Y

I
x
V

G
x
A

P
|
P

K
x
P

T
x
S

L
|
L

-
x
R

I
x
M

P
|
P

I
x
P

G
x
T

I
x
V

E
x
R

Q
x
A

Y
x
F

-
x
V

-
x
A

-
x
S

-
x
R

G
|
G

V
x
T

K
|
K

V
x
Q

-
x
E

Y
x
F

T
x
E

D
x
S

L
x
I

A
x
E

E
|
E

G
x
A

L
|
L

K
x
P

D
|
D

V
x
T

D
|
D

V
|
V

V
x
L

I
x
Y

M
|
M

L
x
T

R
|
R

L
x
I

Q
|
Q

R
x
K

E
|
E

R
|
R

M
x
F

A
x
G

G

-

L

-

P

-

S
|
S

E
x
T

G
x
Q

E
|
E

F
x
Y

Y
x
E

R
x
A

L
x
C

F
|
F

G
|
G

-
x
Q

-
x
F

-
x
I

L
|
L

T
|
T

T
x
P

A
x
H

R
x
I

L
x
M

A
x
T

G
x
R

A
|
A

K
|
K

P
x
K

D
x
K

A
x
M

I
x
V

V
|
V

M
|
M

H
|
H

P
|
P

G
x
M

P
|
P

I

-

N

-

R
|
R

G
x
V

V
x
N

E
|
E

I
|
I

E
x
S

S
x
V

A
x
E

V
|
V

A
x
D

D
x
S

G
x
D

K
x
P

H
x
R

S
x
A

V
x
A

I
x
Y

L
x
F

N
x
R

Q
|
Q

V
x
A

T
x
E

Y
x
N

G
|
G

I
x
M

A
x
Y

V
x
I

R
|
R

M
|
M

A
|
A

V
x
L

L
|
L

S
x
A

M
x
T

A
x
V

M
x
L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
33 M → R: in DEE50; uncertain significance; dbSNP:rs751610198
177 R → Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding ; binding ; H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding ; H→A: No zinc-binding and no catalytic activity.
1475 binding
1505 binding
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding ; H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding ; C→S: Reduces dihydroorotase activity.
1614 binding ; H→A: Abolishes dihydroorotase activity.
1637 binding ; E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding
1686 binding ; D→N: Abolishes dihydroorotase activity.
1690 binding ; H→N: 3% of wild-type catalytic activity.
1702 binding
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

2ipoA E. Coli aspartate transcarbamoylase complexed with n-phosphonacetyl-l- asparagine (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2ipoA

query
sites
2ipoA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

L

L

D

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R

R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding n~2~-(phosphonoacetyl)-l-asparagine: S52 (= S69), T53 (= T70), R54 (= R71), T55 (= T72), R105 (= R121), H134 (= H151), R167 (= R184), T168 (≠ V185), R229 (= R239), L267 (≠ G280)

2h3eA Structure of wild-type e. Coli aspartate transcarbamoylase in the presence of n-phosphonacetyl-l-isoasparagine at 2.3a resolution (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2h3eA

query
sites
2h3eA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

L

L

D

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R

R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding (s)-4-amino-4-oxo-3-(2-phosphonoacetamido)butanoic acid: S52 (= S69), T53 (= T70), R54 (= R71), T55 (= T72), R105 (= R121), H134 (= H151), R167 (= R184), R229 (= R239), L267 (≠ G280)

2fzkA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.50 resolution (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2fzkA

query
sites
2fzkA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S

S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V
x
T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

L

L

D

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R
|
R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding 3,5-bis[(phosphonoacetyl)amino]benzoic acid: T55 (= T72), H134 (= H151), Q137 (= Q154), T168 (≠ V185), R229 (= R239), P266 (= P279), L267 (≠ G280), R296 (= R311)

2fzgA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.25 resolution (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2fzgA

query
sites
2fzgA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S
|
S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

L

L

S

G

A

A

D

S

V

V

I

V

S

G

L

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

T

G

S

K

K
|
K

G

G

E

E

T

T

L

L

F

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

A

V

A

-

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

S

P

D

Q

S

E

G

G

A

A

H

R

F

-

I

L

A

A

E

T

H

E

V

F

C

S

P

G

E

N

V

V

A

P

V

V

I

L

N

N

G

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

G

T

M

L

L

L

D

D

M

L

L

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

S

R

F

L

E

D

N

N

L

L

S

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

D

L

M

T

L

Q

A

A

L

L

K

A

A

K

L

F

G

D

C

G

P

N

D

R

I

F

R

Y

V

F

I

I

G

A

P

P

K

D

T

A

L

L

-

A

I

M

P

P

I

Q

G

Y

I

I

-

L

-

D

-

M

-

L

E

D

Q

E

Y

K

G

G

V

I

-

A

-

W

K

S

V

L

Y

H

T

S

D

S

L

I

A

E

E

E

G

V

L

M

K

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

-

A

-

G

-

L

L

L

D

P

P

S

S

E

E

-

Y

G

A

E

N

F

V

Y

K

R

A

L

Q

F

F

G

V

L

L

T

R

T

A

A

S

R

D

L

L

A

H

G

N

A

A

K

K

P

A

D

N

A

M

I

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

K

P

H

H

S

A

V

W

I

Y

L

F

N

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

A

F

V

A

R

R

M

Q

A

A

V

L

L

L

S

A

M

L

A

V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding {1,3-phenylenebis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S69), R54 (= R71), T55 (= T72), R105 (= R121), H134 (= H151), R167 (= R184), T168 (≠ V185), R229 (= R239), P266 (= P279), L267 (≠ G280)

2fzcA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.10 resolution (see paper)
35% identity, 90% coverage: 19:320/334 of query aligns to 7:305/310 of 2fzcA

query
sites
2fzcA

R

K

H

H

F

I

L

I

S

S

L

I

D

N

G

D

L

L

P

S

R

R

E

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

D

A

S

K

F

L

L

-

E

-

V

-

G

-

A

-

R

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

V

I

C

A

N

S

V

C

F

F

E

E

N

A

S
|
S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

Q

H

R

R

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K
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K

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L

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D

V

V

A

D

D

K

G

T

K

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H

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W

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Y

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N

G
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G

I

I

A

F

V

A

R

R

M

Q

A

A

V

L

L

L

S

A

M

L

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V

M

L

S

N

active site: R54 (= R71), T55 (= T72), K84 (= K99), R105 (= R121), H134 (= H151), Q137 (= Q154), T228 (≠ L238), P266 (= P279), G292 (= G307)
binding {ethane-1,2-diylbis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S69), R54 (= R71), T55 (= T72), R105 (= R121), R167 (= R184), T168 (≠ V185), P266 (= P279), L267 (≠ G280)

Query Sequence

>PP_4998 FitnessBrowser__Putida:PP_4998
MTPIDAKRPLQLNDQGQLRHFLSLDGLPRELLTEILDTADSFLEVGARAVKKVPLLRGKT
VCNVFFENSTRTRTTFELAAQRLSADVISLNVSTSSTSKGETLFDTLRNLEAMAADMFVV
RHSDSGAAHFIAEHVCPEVAVINGGDGRHAHPTQGMLDMLTIRRHKGSFENLSVAIVGDI
LHSRVARSDMLALKALGCPDIRVIGPKTLIPIGIEQYGVKVYTDLAEGLKDVDVVIMLRL
QRERMAGGLLPSEGEFYRLFGLTTARLAGAKPDAIVMHPGPINRGVEIESAVADGKHSVI
LNQVTYGIAVRMAVLSMAMSGQNAQRQLDQENAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory