SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_5184 FitnessBrowser__Putida:PP_5184 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
45% identity, 97% coverage: 12:455/458 of query aligns to 26:469/472 of P78061

query
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P78061

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H282 (= H268) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R343) mutation to Q: Activity is impaired to 3% of wild-type.

8wwuB Glutamine synthetase (see paper)
31% identity, 91% coverage: 26:442/458 of query aligns to 34:465/492 of 8wwuB

query
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binding phosphoaminophosphonic acid-adenylate ester: G157 (≠ A144), E159 (= E146), R226 (≠ D206), F241 (≠ L221), V243 (= V224), H290 (= H270), S292 (= S272), K360 (≠ R338), R365 (= R343), R376 (= R353)
binding magnesium ion: E159 (= E146), E238 (= E218)
binding manganese (ii) ion: E159 (= E146), E161 (= E148), E231 (= E211), E238 (= E218), H288 (= H268), E378 (= E355)

8wwvA Glutamine synthetase (see paper)
31% identity, 91% coverage: 26:442/458 of query aligns to 32:463/490 of 8wwvA

query
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Q

P

S

M

L

L

P

P

N

T

L

L

R

A

D

E

A

A

L

L

R

D

E

A

L

L

D

E

-

H

D

D

S

S

E

E

I

L

L

F

N

R

K

S

Y

C

I

F

D

G

P

D

K

T

Y

F

I

I

D

K

I

Y

F

W

V

L

A

Q

C

L

K

R

E

R

S

S

E

E

L

W

E

A

E

R

F

F

binding adenosine-5'-diphosphate: G155 (≠ A144), E157 (= E146), R224 (≠ D206), F239 (≠ L221), D240 (≠ H222), V241 (= V224), H288 (= H270), S290 (= S272), R374 (= R353), E376 (= E355)
binding magnesium ion: E157 (= E146), E236 (= E218)
binding manganese (ii) ion: E157 (= E146), E159 (= E148), E229 (= E211), E236 (= E218), H286 (= H268), E376 (= E355)
binding l-methionine-s-sulfoximine phosphate: E157 (= E146), E159 (= E148), E229 (= E211), E236 (= E218), A282 (≠ G264), H286 (= H268), R340 (= R320), K358 (≠ R338)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
29% identity, 96% coverage: 17:455/458 of query aligns to 10:436/439 of 7tdpA

query
sites
7tdpA

L

I

K

A

E

E

H

E

P

E

E

N

V

V

L

R

Y

F

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

L

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

E

K

K

V

A

Y

L

E

D

K

N

G

K

I

M

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

Y

G

-

L

-

G

-

L

V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

Y

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

V

E

F

P

P

W

W

Q

S

K

D

R

R

P

-

T

V

A

A

Q

R

L

L

L

I

M

C

T

D

M

I

H

Y

E

K

L

P

E

D

G

G

E

S

P

P

F

F

F

A

A

G

D

D

P

P

R

R

E

G

V

I

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

A

D

E

E

E

L

L

G

G

L

Y

T

T

-

S

I

M

C
x
N

A

V

A
x
G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

T

E

D

N

E

V

-

N

K

G

G

R

D

P

P

Q

T

P

T

P

E

R

L

S

N

P

D

I

Q

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

L

D

A

D

P

L

M

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

E

I

I

L

V

E

L

G

K

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D
x
E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N
x
D

L

F

H

K

H
x
Y

V

-

A

A

D

D

P

A

L

V

K

K

A

A

C

A

D

D

Y

Q

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

Q

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A

N

G
|
G

N

S

G

G

L

M

H
|
H

V

C

H
x
N

I

Q

S
|
S

V

L

L

F

D

-

K

K

D

D

G

N

N

E

N

N

I

V

F

F

T

Y

S

D

E

E

D

T

P

D

E

E

Q

L

-

G

-

L

N

S

A

Q

A

T

L

A

R

R

H

H

A

Y

V

M

G

A

G

G

V

I

L

L

E

K

T

H

L

A

P

R

A

A

S

M

M

A

A

A

F

I

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

G

V

A

P

Q

G

F

Y

Y
x
E

V

A

P

P

N

C

A

Y

P

V

S

A

W

W

G

S

L

A

D

S

N

N

R
|
R

T

S

V

P

A

M

L

I

R
|
R

V

I

P

P

T

A

G

S

S

R

A

G

D

L

A

S

V

T

R
|
R

I

V

E
|
E

H

V

R
|
R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

M

A

M

L

A

A

A

V

V

M

L

L

A

R

G

A

V

G

H

L

H

D

G

G

L

I

T

K

N

R

K

Q

I

M

E

A

P

L

G

P

A

A

P

P

I

I

E

D

G

R

N

N

S

I

Y

Y

-

V

-

M

-

S

-

E

E

E

Q

R

L

I

E

E

Q

E

-

G

-

I

-

P

S

S

L

L

P

P

N

A

N

D

L

L

R

K

D

E

A

A

L

L

R

S

E

E

L

L

D

I

D

R

S

S

E

E

I

V

L

I

N

S

K

D

Y

A

I

L

D

G

P

D

K

H

Y

A

I

L

D

A

I

Y

F

F

V

Y

A

E

C

L

K

K

E

E

S

I

E

E

L

W

E

D

E

M

F

Y

E

R

H

T

S

Q

I

V

S

H

D

Q

L

W

E

E

Y

R

N

D

W

Q

Y

Y

L

L

binding adenosine-5'-diphosphate: N123 (≠ C142), G125 (≠ A144), E127 (= E146), E179 (≠ D206), D193 (≠ N220), Y196 (≠ H223), N242 (≠ H270), S244 (= S272), R316 (= R343), R326 (= R353)
binding magnesium ion: E127 (= E146), E127 (= E146), E129 (= E148), E184 (= E211), E191 (= E218), E191 (= E218), H240 (= H268), E328 (= E355)
binding l-methionine-s-sulfoximine phosphate: E127 (= E146), E129 (= E148), E184 (= E211), E191 (= E218), G236 (= G264), H240 (= H268), R293 (= R320), E299 (≠ Y326), R311 (= R338), R330 (= R357)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
29% identity, 89% coverage: 49:455/458 of query aligns to 36:444/446 of 8ooqB

query
sites
8ooqB

K

K

V

A

Y

G

E

E

K

K

G

G

I

I

N

E

L

E

P

L

A

R

S

D

L

V

F

L

-

E

-

N

A

G

L

L

D

Y

I

F

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

G

I

I

G

N

D

E

A

S

D

D

R

M

I

M

C

L

Y

K

P

P

I

D

P

L

D

S

T

T

L

F

C

S

N

V

E

L

P

P

W

W

Q
x
R

-

P

-

S

K

E

R

K

P

S

T
x
V

A

A

Q

R

L

V

L

I

M

C

T

D

M

V

H

Y

E

T

L

T

E

K

G

G

E

K

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

G

V

C

L

L

R

K

Q

R

V

V

V

M

T

E

K

E

F

F

D

K

-

K

E

E

L

F

G

N

L

G

T

E

I

Y

C

F

A

V

A

G

F

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

I

L

D

K

Q

K

E

D

N

-

V

-

N

-

G

-

R

-

P

P

Q

H

P

N

P

P

R

H

S

K

P

Y

I

I

S

-

G

-

K

-

R

-

P

P

Q

A

S

D

T

D

Q

G

V

G

Y

Y

L

F

-

D

I

L

D

E

D

P

L

M

D

D

E

E

Y

A

A
x
P

D

D

C

I

L
x
R

Q
x
R

D

D

I

I

L

V

E

F

G

A

A

L

K

E

E

N

Q

L

G

G

I

F

P

H

A

V

D

E

A

A

I
x
S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

V

N

D

L

F

H

K

H

-

V

F

A

D

D

D

P

A

L

L

K

K

A

T

C

A

D

D

Y

S

A

V

V

I

L

T

L

F

K

K

R

T

L

T

I

I

K

K

N

T

I

I

A

A

Y

E

D

Q

H

H

E

G

M

L

D

K

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

P

F

G

G

Q

M

A

N

G

G

N

S

G

G

L

M

H

H

V

C

H

H

I

Q

S

S

V

I

L

W

D

L

K

N

D

G

G

E

N

P

N

S

I

F

F

Y

T

D

S

E

E

N

D

A

P

P

E

Y

Q

Q

-

L

N

S

A

E

A

T

L

C

R

M

H

N

A

Y

V

V

G

A

G

G

V

I

L

L

E

K

T

H

L

A

P

K

A

A

S

I

M

V

A

A

F

I

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

N

P

I

S

A

W

W

G

A

L

N

D

S

N

N

R

R

T

S

V

A

A

I

L

I

R

R

V

V

P

P

T

A

G

A

S

R

A

G

D

K

A

G

V

T

R

R

I

I

E

E

H

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

T

A

V

V

M

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

V

T

K

N

N

K

K

I

L

E

D

P

A

G

P

A

E

P

P

I

V

E

E

G

R

N

N

S

I

Y

F

E

A

Q

M

L

S

E

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

Q

E

S

S

L

V

P

P

N

A

N

N

L

L

R

K

D

A

A

A

L

L

R

D

E

E

L

L

D

E

D

N

S

N

E

D

I

V

L

L

N

K

K

N

Y

A

I

L

D

G

P

K

K

H

Y

I

I

F

D

E

I

S

F

F

V

L

A

E

C

I

K

K

E

N

S

A

E

E

L

W

E

D

E

S

F

F

E

R

H

T

S

S

I

V

S

T

D

D

L

W

E

E

Y

T

N

T

W

A

Y

Y

L

L

binding 2-oxoglutaric acid: R86 (≠ Q100), V92 (≠ T104), P169 (≠ A188), R172 (≠ L191), R173 (≠ Q192), S189 (≠ I208)
binding magnesium ion: E137 (= E148), E192 (= E211), E199 (= E218)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
29% identity, 89% coverage: 49:455/458 of query aligns to 37:445/447 of 8oooA

query
sites
8oooA

K

K

V

A

Y

G

E

E

K

K

G

G

I

I

N

E

L

E

P

L

A

R

S

D

L

V

F

L

-

E

-

N

A

G

L

L

D

Y

I

F

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

G

I

I

G

N

D

E

A

S

D

D

R

M

I

M

C

L

Y

K

P

P

I

D

P

L

D

S

T

T

L

F

C

S

N

V

E

L

P

P

W

W

Q
x
R

-

P

-

S

K

E

R

K

P

S

T
x
V

A

A

Q

R

L

V

L

I

M

C

T

D

M

V

H

Y

E

T

L

T

E

K

G

G

E

K

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

G

V

C

L

L

R

K

Q

R

V

V

V

M

T

E

K

E

F

F

D

K

-

K

E

E

L

F

G

N

L

G

T

E

I

Y

C

F

A

V

A

G

F

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

I

L

D

K

Q

K

E

D

N

-

V

-

N

-

G

-

R

-

P

P

Q

H

P

N

P

P

R

H

S

K

P

Y

I

I

S

-

G

-

K

-

R

-

P

P

Q

A

S

D

T

D

Q

G

V

G

Y

Y

L

F

-

D

I

L

D

E

D

P

L

M

D

D

E

E

Y

A

A
x
P

D

D

C

I

L
x
R

Q
x
R

D

D

I

I

L

V

E

F

G

A

A

L

K

E

E

N

Q

L

G

G

I

F

P

H

A

V

D
x
E

A

A

I
x
S

V

H

K

H

E

E

S

V

A

A

P

P

A

G

Q

Q

F

H

E

E

V

V

N

D

L
x
F

H
x
K

H

-

V
x
F

A

D

D

D

P

A

L

L

K

K

A

T

C

A

D

D

Y

S

A

V

V

I

L

T

L

F

K

K

R

T

L

T

I

I

K

K

N

T

I

I

A

A

Y

E

D

Q

H

H

E

G

M

L

D

K

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

P

F

G

G

Q

M

A

N

G

G

N

S

G

G

L

M

H

H

V

C

H
|
H

I

Q

S
|
S

V

I

L

W

D

L

K

N

D

G

G

E

N

P

N

S

I

F

F

Y

T

D

S

E

E

N

D

A

P

P

E

Y

Q

Q

-

L

N

S

A

E

A

T

L

C

R

M

H

N

A

Y

V

V

G

A

G

G

V

I

L

L

E

K

T

H

L

A

P

K

A

A

S

I

M

V

A

A

F

I

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

N

P

I

S

A

W

W

G

A

L

N

D

S

N

N

R

R

T

S

V

A

A

I

L

I

R
|
R

V

V

P

P

T

A

G

A

S

R

A

G

D

K

A

G

V

T

R
|
R

I

I

E

E

H

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

T

A

V

V

M

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

V

T

K

N

N

K

K

I

L

E

D

P

A

G

P

A

E

P

P

I

V

E

E

G

R

N

N

S

I

Y

F

E

A

Q

M

L

S

E

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

Q

E

S

S

L

V

P

P

N

A

N

N

L

L

R

K

D

A

A

A

L

L

R

D

E

E

L

L

D

E

D

N

S

N

E

D

I

V

L

L

N

K

K

N

Y

A

I

L

D

G

P

K

K

H

Y

I

I

F

D

E

I

S

F

F

V

L

A

E

C

I

K

K

E

N

S

A

E

E

L

W

E

D

E

S

F

F

E

R

H

T

S

S

I

V

S

T

D

D

L

W

E

E

Y

T

N

T

W

A

Y

Y

L

L

binding 2-oxoglutaric acid: R87 (≠ Q100), V93 (≠ T104), P170 (≠ A188), R173 (≠ L191), R174 (≠ Q192), S190 (≠ I208)
binding adenosine-5'-triphosphate: E136 (= E146), E188 (≠ D206), F203 (≠ L221), K204 (≠ H222), F205 (≠ V224), H251 (= H270), S253 (= S272), R325 (= R343), R335 (= R353)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

7tfaB Glutamine synthetase (see paper)
29% identity, 96% coverage: 17:455/458 of query aligns to 10:438/441 of 7tfaB

query
sites
7tfaB

L

I

K

A

E

E

H

E

P

E

E

N

V

V

L

R

Y

F

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

L

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

E

K

K

V

A

Y

L

E

D

K

N

G

K

I

M

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T
x
Y

G

-

L

-

G

-

L

V

D
x
R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

Y

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

V

E

F

P

P

W

W

-

V

Q

T

K

S

R

D

P

R

T

V

A

A

Q

R

L

L

L

I

M

C

T

D

M

I

H

Y

E

K

L

P

E

D

G

G

E

S

P

P

F

F

F

A

A

G

D

D

P

P

R

R

E

G

V

I

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

A

D

E

E

E

L

L

G

G

L

Y

T

T

-

S

I

M

C

N

A

V

A

G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

T

E

D

N

E

V

-

N

K

G

G

R

D

P

P

Q

T

P

T

P

E

R

L

S

N

P

D

I

Q

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V
x
Y

Y

F

L

D

I

L

D

A

D

P

L

M

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

E

I

I

L

V

E

L

G

K

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S
x
V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

K

H

Y

V

-

A

A

D

D

P

A

L

V

K

K

A

A

C

A

D

D

Y

Q

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

Q

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A
x
N

G
|
G

N

S

G

G

L

M

H
|
H

V

C

H

N

I

Q

S

S

V

L

L

F

D

-

K

K

D

D

G

N

N

E

N

N

I

V

F

F

T

Y

S

D

E

E

D

T

P

D

E

E

Q

L

-

G

-

L

N

S

A

Q

A

T

L

A

R

R

H

H

A

Y

V

M

G

A

G

G

V

I

L

L

E

K

T

H

L

A

P

R

A

A

S

M

M

A

A

A

F

I

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

G

V

A

P

Q
x
G

F
x
Y

Y
x
E

V

A

P

P

N

C

A

Y

P

V

S

A

W

W

G

S

L

A

D

S

N

N

R
|
R

T

S

V

P

A

M

L

I

R

R

V

I

P

P

T

A

G

S

S

R

A

G

D

L

A

S

V

T

R

R

I

V

E
|
E

H

V

R

R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

M

A

M

L

A

A

A

V

V

M

L

L

A

R

G

A

V

G

H

L

H

D

G

G

L

I

T

K

N

R

K

Q

I

M

E

A

P

L

G

P

A

A

P

P

I

I

E

D

G

R

N

N

S

I

Y

Y

-

V

-

M

-

S

-

E

E

E

Q

R

L

I

E

E

Q

E

-

G

-

I

-

P

S

S

L

L

P

P

N

A

N

D

L

L

R

K

D

E

A

A

L

L

R

S

E

E

L

L

D

I

D

R

S

S

E

E

I

V

L

I

N

S

K

D

Y

A

I

L

D

G

P

D

K

H

Y

A

I

L

D

A

I

Y

F

F

V

Y

A

E

C

L

K

K

E

E

S

I

E

E

L

W

E

D

E
x
M

F

Y

E

R

H

T

S

Q

I

V

S

H

D

Q

L

W

E

E

Y

R

N

D

W

Q

Y

Y

L

L

binding glutamine: E131 (= E148), Y153 (≠ V178), E186 (= E211), G238 (= G264), H242 (= H268), R295 (= R320), E301 (≠ Y326)
binding magnesium ion: E129 (= E146), E131 (= E148), E186 (= E211), E193 (= E218), H242 (= H268), E330 (= E355)
binding : Y58 (≠ T74), R60 (≠ D79), V187 (≠ S212), N237 (≠ A263), G299 (≠ Q324), Y300 (≠ F325), R313 (= R338), M424 (≠ E441)

4s0rD Structure of gs-tnra complex (see paper)
28% identity, 95% coverage: 23:455/458 of query aligns to 21:444/447 of 4s0rD

query
sites
4s0rD

V

V

L

K

Y

Y

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

G

K

K

V

A

Y

L

E

D

K

N

G

K

I

V

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N
x
D

G

G

S

S

T

S

V

I

E

E

S

G

T
x
F

G

-

L

-

G

-

L
x
V

D
x
R

I
|
I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

Y

P

P

I

D

P

L

D

N

T

T

L

F

C

V

N

I

E

F

P

P

W

W

-

T

-

A

Q

E

K

K

R

G

P

K

T

V

A

A

Q

R

L

F

L

I

M

C

T

D

M

I

H

Y

E

N

L

P

E

D

G

G

E

T

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

N

V

N

L

L

R

K

Q

R

V

I

V

L

T

K

K

E

F

M

D

E

E

D

L

L

G

G

L

F

T

S

-

D

I

F

C

N

A

L

A

G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

K

G

G

R

E

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

K

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L
x
D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

I

I

L

V

E

L

G

E

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

K

H

Y

V

-

A

A

D

G

P

A

L

V

K

R

A

S

C

C

D

D

Y

D

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

K

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G
|
G

Q
x
V

A
x
N

G

G

N

S

G

G

L

M

H
|
H

V

C

H

N

I

L

S

S

V

L

L

F

D

-

K

K

D

N

G

G

N

V

N

N

I

A

F

F

T

F

S

D

E

E

-

N

-

A

D

D

P

L

E

Q

Q

L

N

S

A

E

A

T

L

A

R

K

H

H

A

F

V

I

G

A

G

G

V

I

L

V

E

K

T

H

L

A

P

T

A

S

S

F

M

T

A

A

F

V

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

G

V

A

P

Q
x
G

F
x
Y

Y
x
E

V

A

P

P

N

C

A

Y

P

V

S

A

W

W

G

S

L

A

D

Q

N

N

R
|
R

T

S

V

P

A

L

L

I

R

R

V

I

P

P

T

A

G

S

S

R

A

G

D

I

A

S

V

T

R

R

I

V

E
|
E

H

V

R
|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

M

A

M

L

A

S

A

V

V

L

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

I

T

K

N

N

K

K

I

L

E

E

P

A

G

P

A

A

P

P

I

I

E

D

G

R

N

N

S

I

Y
|
Y

-

V

-

M

-

S

-

K

-

E

E

E

Q

R

L

M

E

E

Q

N

S

G

-

I

-

V

-

D

L

L

P

P

N

A

N

T

L

L

R

A

D

E

A

A

L

L

R

E

E

E

L

F

D

K

D

S

S

N

E

E

I

V

L

M

N

V

K

K

Y

A

I

L

D

G

P

E

K

H

Y

L

I

F

D

E

I
x
H

F

F

V

I

A

E

C

A

K

K

E

E

S
x
I

E

E

L

W

E

D

E
x
M

F

F

E

R

H

T

S

Q

I

V

S

H

D

P

L

W

E

E

Y

R

N

E

W

Q

Y

Y

L

M

active site: D56 (≠ N67), E135 (= E146), E137 (= E148), E192 (= E211), E199 (= E218), H248 (= H268), R319 (= R338), E336 (= E355), R338 (= R357)
binding glutamine: E137 (= E148), E192 (= E211), R301 (= R320), E307 (≠ Y326)
binding magnesium ion: I66 (= I80), E135 (= E146), E135 (= E146), E199 (= E218), H248 (= H268), H248 (= H268), E336 (= E355), H419 (≠ I430)
binding : F63 (≠ T74), V64 (≠ L78), R65 (≠ D79), I66 (= I80), D161 (≠ L180), G241 (= G261), V242 (≠ Q262), N243 (≠ A263), G305 (≠ Q324), Y306 (≠ F325), Y376 (= Y395), I426 (≠ S437), M430 (≠ E441)

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
28% identity, 95% coverage: 23:455/458 of query aligns to 17:440/443 of 4lnkA

query
sites
4lnkA

V

V

L

K

Y

Y

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

G

K
|
K

V

A

Y

L

E

D

K

N

G

K

I

V

N
x
M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N
x
D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

Y

P

P

I

D

P

L

D

N

T

T

L

F

C

V

N

I

E

F

P

P

W

W

-

T

-

A

Q

E

K

K

R

G

P

K

T

V

A

A

Q

R

L

F

L

I

M

C

T

D

M

I

H

Y

E

N

L

P

E

D

G

G

E

T

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

N

V

N

L

L

R

K

Q

R

V

I

V

L

T

K

K

E

F

M

D

E

E

D

L

L

G

G

L

F

T

S

-

D

I

F

C

N

A

L

A

G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

K

G

G

R

E

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

K

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

I

I

L

V

E

L

G

E

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S
x
V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L
x
F

H

K

H
x
Y

V

-

A

A

D

G

P

A

L

V

K

R

A

S

C

C

D

D

Y

D

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

K

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A
x
N

G
|
G

N

S

G
|
G

L

M

H
|
H

V

C

H
x
N

I

L

S
|
S

V

L

L

F

D

-

K

K

D

N

G

G

N

V

N

N

I

A

F

F

T

F

S

D

E

E

-

N

-

A

D

D

P

L

E

Q

Q

L

N

S

A

E

A

T

L

A

R

K

H

H

A

F

V

I

G

A

G

G

V

I

L

V

E

K

T

H

L

A

P

T

A

S

S

F

M

T

A

A

F

V

L

T

C

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y
x
E

V

A

P

P

N

C

A

Y

P

V

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A

W

W

G

S

L

A

D

Q

N

N

R
|
R

T

S

V

P

A

L

L

I

R

R

V

I

P

P

T

A

G
x
S

S

R

A

G

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I

A
x
S

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R
|
R

I

V

E
|
E

H

V

R
|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

M

A

M

L

A

S

A

V

V

L

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

I

T

K

N

N

K

K

I

L

E

E

P

A

G

P

A

A

P

P

I

I

E

D

G

R

N

N

S

I

Y

Y

-

V

-

M

-

S

-

K

-

E

E

E

Q

R

L

M

E

E

Q

N

S

G

-

I

-

V

-

D

L

L

P

P

N

A

N

T

L

L

R

A

D

E

A

A

L

L

R

E

E

E

L

F

D

K

D

S

S

N

E

E

I

V

L

M

N

V

K

K

Y

A

I

L

D

G

P

E

K

H

Y

L

I

F

D

E

I

H

F

F

V

I

A

E

C

A

K

K

E

E

S

I

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E

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D

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M

F

F

E

R

H

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active site: D52 (≠ N67), E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), H244 (= H268), R315 (= R338), E332 (= E355), R334 (= R357)
binding adenosine-5'-diphosphate: K43 (= K49), M50 (≠ N56), F198 (≠ L221), Y200 (≠ H223), N246 (≠ H270), S248 (= S272), S324 (≠ G347), S328 (≠ A351), R330 (= R353)
binding glutamic acid: E133 (= E148), E188 (= E211), V189 (≠ S212), N239 (≠ A263), G240 (= G264), G242 (= G266), E303 (≠ Y326)
binding magnesium ion: E131 (= E146), E188 (= E211), E195 (= E218), H244 (= H268), E332 (= E355)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
28% identity, 95% coverage: 23:455/458 of query aligns to 17:440/443 of 4lniA

query
sites
4lniA

V

V

L

K

Y

Y

V

I

D

R

L

L

L

Q

I

F

A

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G

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K

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I

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R

V

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S

S

Q

L

L

H

G

K

K

V

A

Y

L

E

D

K

N

G

K

I

V

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N
x
D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

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R

I

I

G

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D

E

A

S

D

D

R

M

I

Y

C

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Y

Y

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C

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I

E

F

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W

-

T

-

A

Q

E

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K

R

G

P

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A

A

Q

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L

I

M

C

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M

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N

L

P

E

D

G

G

E

T

P

P

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F

F

E

A

G

D

D

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P

R

R

E

N

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N

L

L

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Q

R

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I

V

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K

K

E

F

M

D

E

E

D

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L

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G

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D

I

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A

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E
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E

L

P

E
|
E

F

F

Y

F

L

L

I

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D

K

Q

L

E

D

N

E

V

-

N

K

G

G

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E

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P

Q

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P

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P

E

R

L

S

N

P

D

I

K

S

G

G

G

K

-

R

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Y

F

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I

L

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A

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E

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E

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E

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x
D

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x
Y

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A

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A

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Y

D

A

I

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T

F

F

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M

A

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K

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A

N

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H

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x
N

I

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S

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L

L

F

D

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K

K

D

N

G

G

N

V

N

N

I

A

F

F

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F

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D

E

E

-

N

-

A

D

D

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E

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Q

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A

E

A

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H

H

A

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G

A

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K

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H

L

A

P

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A

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F

M

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A

A

F

V

L

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C

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R
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R

F

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x
E

V

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P

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C

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R

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R

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H

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R

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D

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D

A

A

A

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Y

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V

L

L

L

A

A

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A

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G

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N

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P

I

I

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Y

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M

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E

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I

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A

A

L

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R

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E

E

L

F

D

K

D

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S

N

E

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A

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E

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F

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A

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A

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K

E

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F

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active site: D52 (≠ N67), E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), H244 (= H268), R315 (= R338), E332 (= E355), R334 (= R357)
binding adenosine-5'-diphosphate: E131 (= E146), E183 (≠ D206), D197 (≠ N220), Y200 (≠ H223), N246 (≠ H270), S248 (= S272), R320 (= R343), R330 (= R353)
binding magnesium ion: E131 (= E146), E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), E195 (= E218), H244 (= H268), E332 (= E355)
binding l-methionine-s-sulfoximine phosphate: E133 (= E148), E188 (= E211), H244 (= H268), R297 (= R320), E303 (≠ Y326), R315 (= R338), R334 (= R357)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
28% identity, 95% coverage: 23:455/458 of query aligns to 18:441/444 of P12425

query
sites
P12425

V

V

L

K

Y

Y

V

I

D

R

L

L

L

Q

I

F

A

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D

D

M

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G

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K

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I

I

E

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V

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S

S

Q

L

L

H

G

K

K

V

A

Y

L

E

D

K

N

G

K

I

V

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S
x
G

T

F

G

-

L

-

G

-

L

V

D
x
R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

Y

P

P

I

D

P

L

D

N

T

T

L

F

C

V

N

I

E

F

P

P

W

W

-

T

-

A

Q

E

K

K

R

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P

K

T

V

A

A

Q

R

L

F

L

I

M

C

T

D

M

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N

L

P

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D

G

G

E

T

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P

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F

F

E

A

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D

P

P

R

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N

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N

L

L

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Q

R

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I

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T

K

K

E

F

M

D

E

E

D

L

L

G

G

L

F

T

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-

D

I

F

C

N

A

L

A

G

F

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E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

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-

N

K

G

G

R

E

P

P

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T

P

L

P

E

R

L

S

N

P

D

I

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G

G

G

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R

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Y

F

L

D

I

L

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A

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A

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V

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E

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D

Y

D

A

I

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L

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T

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F

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M

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K

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Y

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G

G

Q

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A

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G
|
G

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G

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H
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H

V

C

H

N

I

L

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S

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L

L

F

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K

K

D

N

G

G

N

V

N

N

I

A

F

F

T

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E

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-

A

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A

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S

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A

A

F

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V

N

N

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S

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Y

R

K

R

R

F

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x
G

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x
E

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|
P

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G

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N

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R

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E

H

V

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R

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D

A

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D

A

A

A

N

N

P

P

Y

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L

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A

M

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A

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V

L

L

L

A

A

G

A

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G

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D

G

G

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N

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I

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E

E

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A

A

P

P

I

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D

G

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N

N

S

I

Y

Y

-

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-

M

-

S

-

K

-

E

E

E

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R

L

M

E

E

Q

N

S

G

-

I

-

V

-

D

L

L

P

P

N

A

N

T

L

L

R

A

D

E

A

A

L

L

R

E

E

E

L

F

D

K

D

S

S

N

E

E

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N

V

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I

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K

E

E

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E

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F

F

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M

G59 (≠ S73) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ D79) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E146) binding Mg(2+)
E134 (= E148) binding Mg(2+)
E189 (= E211) binding Mg(2+)
V190 (≠ S212) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E218) binding Mg(2+)
G241 (= G264) binding L-glutamate
H245 (= H268) binding Mg(2+)
G302 (≠ Q324) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ Y326) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P328) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E355) binding Mg(2+)
E424 (= E438) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8ooxB Glutamine synthetase (see paper)
29% identity, 96% coverage: 19:457/458 of query aligns to 11:437/438 of 8ooxB

query
sites
8ooxB

E

E

H

E

P

K

E

N

V

V

L

R

Y

F

V

V

D

R

L

L

L

Q

I

F

A

V

D

D

M

V

N

Q

G

G

V

I

V

P

R

K

G

N

K

V

R

A

I

I

E

P

R

V

T

G

S

Q

L

L

H

E

K

K

V

A

Y

L

E

G

K

P

G

G

I

I

N

H

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

V

C

L

Y

R

P

P

I

D

P

P

D

D

T

T

L

F

C

R

N

V

E

L

P

P

W

W

Q

S

K

G

R

N

P

T

T

A

-

E

A

A

Q

R

L

L

L

I

M

C

T

D

M

I

H

E

E

L

L

P

E

D

G

G

E

K

P

P

F

F

F

M

A

G

D

C

P

P

R

R

E

Q

V

V

L

L

R

K

Q

K

V

N

V

M

T

E

K

E

F

A

D

A

E

K

L

L

G

G

L

Y

T

V

I

M

C

N

A

T

A

G

F

P

E

E

L

M

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

R

E

Q

N

D

V

-

N

-

G

G

R

M

P

P

Q

T

P

-

R

-

S

-

P

-

I

-

S

-

G

-

K

N

R

I

P

P

Q

Q

S

D

T

R

Q

G

V

G

Y

Y

L

F

-

D

I

L

D

A

D

P

L

I

D

D

E

L

Y

A

A

E

D

E

C

I

L

K

Q

R

D

E

I

I

L

V

E

L

G

V

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

V

D

E

A

A

I

A

V

H

K

H

E
|
E

S

V

A

A

P

F

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

K

H

Y

V

-

A

D

D

N

P

A

L

L

K

A

A

T

C

A

D

D

Y

N

A

V

V

I

L

T

L

L

K

K

R

Y

L

V

I

A

K

K

N

T

I

L

A

A

Y

L

D

Q

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

I

P

F

G

G

Q

V

A

N

G

G

N

S

G

G

L

M

H

H

V

T

H

N

I

T

S

S

V

L

L

F

D

-

K

K

D

D

G

G

N

K

N

N

I

A

F

F

T

Y

S

D

E

P

D

D

-

A

P

P

E

D

Q

Q

-

I

N

S

A

D

A

T

L

L

R

R

H

Y

A

F

V

V

G

G

V

V

L

L

E

K

T

H

L

I

P

R

A

A

S

I

M

T

A

A

F

I

L

T

C

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

Y

P

I

S

T

W

W

G

S

L

G

D

P

N

N

R

R

T

S

V

S

A

L

L

I

R

R

V

V

P

P

T

A

G

P

S

R

A

G

D

N

A

S

V

T

R

R

I

I

E

E

H

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

A

V

I

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

V

T

K

N

N

K

K

I

I

E

E

P

P

G

P

A

E

P

R

I

V

E

E

G

K

N

N

S

I

Y

Y

E

K

Q

L

L

T

E

E

Q

E

S

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

N

G

N

T

L

L

R

K

D

E

A

A

L

I

R

E

E

C

L

F

D

K

D

E

S

D

E

E

I

L

L

L

N

V

K

S

Y

A

I

L

D

G

P

E

K

H

Y

V

I

S

D

Q

I

S

F

I

V

I

A

N

C

V

K

A

E

M

S

A

E

D

L

W

E

D

E

S

F

Y

E

R

H

T

S

Q

I

V

S

H

D

Q

L

W

E

E

Y

L

N

D

W

R

Y

Y

L

L

H

Q

T

T

binding magnesium ion: E129 (= E148), E183 (= E211), E190 (= E218)

7tenA Glutamine synthetase (see paper)
28% identity, 95% coverage: 23:455/458 of query aligns to 16:439/442 of 7tenA

query
sites
7tenA

V

V

L

K

Y

F

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

K

K

K

V

A

Y

L

E

D

K

N

G

K

I

I

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

F

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

V

E

F

P

P

W

W

-

T

-

A

Q

E

K

K

R

G

P

K

T

V

A

A

Q

R

L

M

L

I

M

C

T

D

M

I

H

Y

E

N

L

P

E

D

G

M

E

T

P

P

F

F

F

A

A

G

D

D

P

P

R

R

E

A

V

N

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

M

D

E

E

E

L

L

G

G

L

F

T

T

-

E

I

F

C

N

A

L

A
x
G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

N

G

R

R

R

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

S

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

I

I

L

V

E

L

G

E

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D
x
E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N
x
D

L
x
F

H
x
K

H
x
Y

V

-

A

E

D

D

P

A

L

I

K

T

A

A

C

C

D

D

Y

S

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

K

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A
x
N

G
|
G

N

S

G

G

L

M

H
|
H

V

F

H
x
N

I

M

S
|
S

V

L

L

F

D

N

K

E

D

K

G

G

N

N

N

A

I

F

F

F

-

D

T

E

S

S

E

G

D

E

P

L

E

E

Q

L

N

S

A

Q

A

T

L

A

R

Y

H

H

A

F

V

L

G

A

G

G

V

M

L

L

E

K

T

H

L

A

P

R

A

G

S

Y

M

T

A

A

F

V

L

T

C

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

F

L

G

V

A

P

Q

G

F

Y

Y
x
E

V

A

P

P

N

C

A

Y

P

I

S

A

W

W

G

S

L

G

D

K

N

N

R
|
R

T

S

V

P

A

L

L

V

R
|
R

V

V

P

P

T

S

G

S

S

R

A

G

D

L

A
x
S

V

T

R
|
R

I

L

E

E

H

L

R
|
R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

M

A

M

M

A

A

A

V

V

L

L

L

A

K

G

A

V

G

H

L

H

S

G

G

L

I

T

K

N

D

K

E

I

L

E

T

P

P

G

P

A

A

P

P

I

V

E

D

G

R

N

N

S

I

Y

Y

E

G

Q

M

L

N

E

E

Q

E

S

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

-

D

L

L

P

P

N

E

N

S

L

L

R

G

D

H

A

A

L

L

R

I

E

E

L

L

D

E

D

K

S

N

E

E

I

I

L

I

N

K

K

D

Y

G

I

L

D

G

P

E

K

H

Y

I

I

F

D

E

I

H

F

F

V

I

A

E

C

A

K

K

E

T

S

I

E

E

L

C

E

D

E

M

F

F

E

R

H

T

S

A

I

V

S

H

D

P

L

W

E

E

Y

R

N

E

W

Q

Y

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A144), E130 (= E146), E182 (≠ D206), D196 (≠ N220), F197 (≠ L221), K198 (≠ H222), Y199 (≠ H223), N245 (≠ H270), S247 (= S272), R319 (= R343), S327 (≠ A351), R329 (= R353)
binding l-methionine-s-sulfoximine phosphate: E130 (= E146), E132 (= E148), E187 (= E211), E194 (= E218), N238 (≠ A263), G239 (= G264), H243 (= H268), R296 (= R320), E302 (≠ Y326), R314 (= R338), R333 (= R357)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
28% identity, 95% coverage: 23:455/458 of query aligns to 17:440/443 of 7tf9S

query
sites
7tf9S

V

V

L

K

Y

F

V

I

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

I

R

K

G

N

K

V

R

E

I

I

E

P

R

V

T

S

S

Q

L

L

H

K

K

K

V

A

Y

L

E

D

K

N

G

K

I

I

N

M

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T
x
F

G

-

L

-

G

-

L
x
V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

F

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

V

E

F

P

P

W

W

-

T

-

A

Q

E

K

K

R

G

P

K

T

V

A

A

Q

R

L

M

L

I

M

C

T

D

M

I

H

Y

E

N

L

P

E

D

G

M

E

T

P

P

F

F

F

A

A

G

D

D

P

P

R

R

E

A

V

N

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

M

D

E

E

E

L

L

G

G

L

F

T

T

-

E

I

F

C

N

A

L

A

G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

N

G

R

R

R

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

S

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V
x
Y

Y

F

L

D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

I

I

L

V

E

L

G

E

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

K

H

Y

V

-

A

E

D

D

P

A

L

I

K

T

A

A

C

C

D

D

Y

S

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

K

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A

N

G
|
G

N

S

G
|
G

L

M

H
|
H

V

F

H

N

I

M

S

S

V

L

L

F

D

N

K

E

D

K

G

G

N

N

N

A

I

F

F

F

-

D

T

E

S

S

E

G

D

E

P

L

E

E

Q

L

N

S

A

Q

A

T

L

A

R

Y

H

H

A

F

V

L

G

A

G

G

V

M

L

L

E

K

T

H

L

A

P

R

A

G

S

Y

M

T

A

A

F

V

L

T

C

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

F

L

G

V

A

P

Q

G

F

Y

Y
x
E

V

A

P

P

N

C

A

Y

P

I

S

A

W

W

G

S

L

G

D

K

N

N

R

R

T

S

V

P

A

L

L

V

R

R

V

V

P

P

T

S

G

S

S

R

A

G

D

L

A

S

V

T

R

R

I

L

E
|
E

H

L

R

R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

M

A

M

M

A

A

A

V

V

L

L

L

A

K

G

A

V

G

H

L

H

S

G

G

L

I

T

K

N

D

K

E

I

L

E

T

P

P

G

P

A

A

P

P

I

V

E

D

G

R

N

N

S

I

Y

Y

E

G

Q

M

L

N

E

E

Q

E

S

E

-

R

-

E

-

A

-

T

-

G

-

I

-

Y

-

D

L

L

P

P

N

E

N

S

L

L

R

G

D

H

A

A

L

L

R

I

E

E

L

L

D

E

D

K

S

N

E

E

I

I

L

I

N

K

K

D

Y

G

I

L

D

G

P

E

K

H

Y

I

I

F

D

E

I

H

F

F

V

I

A
x
E

C

A

K

K

E

T

S
x
I

E

E

L

C

E

D

E
x
M

F

F

E

R

H

T

S

A

I

V

S

H

D

P

L

W

E

E

Y

R

N

E

W

Q

Y

Y

L

L

binding glutamine: E133 (= E148), Y155 (≠ V178), E188 (= E211), G240 (= G264), G242 (= G266), R297 (= R320), E303 (≠ Y326)
binding magnesium ion: E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), H244 (= H268), E332 (= E355)
binding : F59 (≠ T74), V60 (≠ L78), E418 (≠ A433), I422 (≠ S437), M426 (≠ E441)

8oozA Glutamine synthetase (see paper)
29% identity, 96% coverage: 19:457/458 of query aligns to 11:429/430 of 8oozA

query
sites
8oozA

E

E

H

E

P

K

E

N

V

V

L

R

Y

F

V

V

D

R

L

L

L

Q

I

F

A

V

D

D

M

V

N

Q

G

G

V

I

V

P

R

K

G

N

K

V

R

A

I

I

E

P

R

V

T

G

S

Q

L

L

H

E

K

K

V

A

Y

L

E

G

K

P

G

G

I

I

N

H

L

F

P

-

A

-

S

-

L

-

F

-

A

-

L

-

D

-

I

-

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

S

G

-

L

-

G

-

L

-

D

-

I

-

G

-

D

-

A

-

D

D

R

M

I

V

C

L

Y

R

P

P

I

D

P

P

D

D

T

T

L

F

C

R

N

V

E

L

P

P

W

W

Q

S

K

T

R

-

P

A

T

E

A

A

Q

R

L

L

L

I

M

C

T

D

M

I

H

E

E

L

L

P

E

D

G

G

E

K

P

P

F

F

F

M

A

G

D

C

P

P

R

R

E

Q

V

V

L

L

R

K

Q

K

V

N

V

M

T

E

K

E

F

A

D

A

E

K

L

L

G

G

L

Y

T

V

I

M

C

N

A

T

A
x
G

F

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

I

F

D

K

Q

R

E

Q

N

D

V

-

N

-

G

G

R

M

P

P

Q

T

P

-

R

-

S

-

P

-

I

-

S

-

G

-

K

N

R

I

P

P

Q

Q

S

D

T

R

Q

G

V

G

Y

Y

L

F

-

D

I

L

D

A

D

P

L

I

D

D

E

L

Y

A

A

E

D

E

C

I

L

K

Q

R

D

E

I

I

L

V

E

L

G

V

A

L

K

E

E

E

Q

M

G

G

I

F

P

E

A

V

D
x
E

A

A

I

A

V

H

K

H

E

E

S

V

A

A

P

F

A

G

Q

Q

F

H

E

E

V

I

N

D

L
x
F

H
x
K

H
x
Y

V

-

A

D

D

N

P

A

L

L

K

A

A

T

C

A

D

D

Y

N

A

V

V

I

L

T

L

L

K

K

R

Y

L

V

I

A

K

K

N

T

I

L

A

A

Y

L

D

Q

H

H

E

G

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

I

P

F

G

G

Q

V

A

N

G

G

N

S

G

G

L

M

H
|
H

V

T

H
x
N

I

T

S
|
S

V

L

L

F

D

-

K

K

D

D

G

G

N

K

N

N

I

A

F

F

T

Y

S

D

E

P

D

D

-

A

P

P

E

D

Q

Q

-

I

N

S

A

D

A

T

L

L

R

R

H

Y

A

F

V

V

G

G

V

V

L

L

E

K

T

H

L

I

P

R

A

A

S

I

M

T

A

A

F

I

L

T

C

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

Y

P

I

S

T

W

W

G

S

L

G

D

P

N

N

R

R

T

S

V

S

A

L

L

I

R

R

V

V

P

P

T

A

G

P

S

R

A

G

D

N

A
x
S

V

T

R
|
R

I

I

E
|
E

H

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

A

V

I

L

L

A

A

G

A

V

G

H

L

H

D

G

G

L

V

T

K

N

N

K

K

I

I

E

E

P

P

G

P

A

E

P

R

I

V

E

E

G

K

N

N

S

I

Y

Y

E

K

Q

L

L

T

E

E

Q

E

S

E

-

R

-

E

-

K

-

L

-

G

-

I

-

G

-

M

L

L

P

P

N

G

N

T

L

L

R

K

D

E

A

A

L

I

R

E

E

C

L

F

D

K

D

E

S

D

E

E

I

L

L

L

N

V

K

S

Y

A

I

L

D

G

P

E

K

H

Y

V

I

S

D

Q

I

S

F

I

V

I

A

N

C

V

K

A

E

M

S

A

E

D

L

W

E

D

E

S

F

Y

E

R

H

T

S

Q

I

V

S

H

D

Q

L

W

E

E

Y

L

N

D

W

R

Y

Y

L

L

H

Q

T

T

binding adenosine-5'-triphosphate: G117 (≠ A144), E170 (≠ D206), F185 (≠ L221), K186 (≠ H222), Y187 (≠ H223), N233 (≠ H270), S235 (= S272), S315 (≠ A351), R317 (= R353)
binding magnesium ion: E119 (= E146), H231 (= H268), E319 (= E355)

7tf6A Glutamine synthetase (see paper)
28% identity, 95% coverage: 19:455/458 of query aligns to 12:435/438 of 7tf6A

query
sites
7tf6A

E

E

H

E

P

E

E

N

V

V

L

R

Y

Y

V

L

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

V

E

P

R

V

T

S

S

Q

L

L

H

E

K

K

V

V

Y

L

E

D

K

N

G

-

I

-

N

-

L

-

P

-

A

-

S

-

L

-

F

-

A

E

L

M

D

M

I

F

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T
x
F

G

-

L

-

G

-

L

V

D
x
R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

H

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

I

E

F

P

P

W

W

Q

G

K

K

R

-

P

-

T

V

A

A

Q

R

L

L

L

I

M

C

T

D

M

V

H

Y

E

K

L

T

E

D

G

G

E

T

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

A

V

N

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

M

D

E

E

D

L

L

G

G

L

F

T

T

-

D

I

F

C

N

A

L

A

G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

K

G

G

R

E

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

D

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

I

I

L

V

E

L

G

E

A

L

K

E

E

D

Q

M

G

G

I

F

P

D

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

G

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

K

H

Y

V

-

A

A

D

D

P

A

L

V

K

T

A

A

C

C

D

D

Y

N

A

I

V

Q

L

T

L

F

K

K

R

L

L

V

I

V

K

K

N

T

I

I

A

A

Y

R

D

K

H

H

E

N

M

L

D

H

T

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G
|
G

Q

V

A
x
N

G
|
G

N

S

G

G

L

M

H
|
H

V

F

H

N

I

V

S

S

V

L

L

F

D

K

K

G

D

K

G

E

N

N

N

A

I

F

F

F

T

-

S

-

E

-

D

D

P

P

E

N

Q

T

N

E

A

M

A

G

L

L

R

T

H

E

A

T

-

A

-

Y

-

Q

-

F

V

T

G

A

G

G

V

V

L

L

E

K

T

N

L

A

P

R

A

G

S

F

M

T

A

A

F

V

L

C

C

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

G

V

A

P

Q
x
G

F
x
Y

Y
x
E

V

A

P

P

N

C

A

Y

P

I

S

A

W

W

G

S

L

G

D

K

N

N

R
|
R

T

S

V

P

A

L

L

I

R

R

V

V

P

P

T

S

G

S

S

R

A

G

D

L

A

S

V

T

R

R

I

I

E
|
E

H

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

M

A

M

L

A

A

V

I

L

L

A

E

G

A

V

G

H

L

H

D

G

G

L

I

T

K

N

N

K

K

I

L

E

K

P

V

G

P

A

E

P

P

I

V

E

N

G

Q

N

N

S

I

Y
|
Y

E

E

Q

M

L

N

E

R

-

E

-

R

-

E

-

A

-

V

-

G

-

I

Q

Q

S

D

L

L

P

P

N

S

N

T

L

L

R

Y

D

T

A

A

L

L

R

K

E

A

L

M

D

R

D

E

S

N

E

E

I

V

L

I

N

K

K

K

Y

A

I

L

D

G

P

N

K

H

Y

I

I

Y

D

N

I

Q

F

F

V

I

A

N

C

S

K

K

E

S

S

I

E

E

L

W

E

D

E
x
Y

F

Y

E

R

H

T

S

Q

I

V

S

S

D

E

L

W

E

E

Y

R

N

D

W
x
Q

Y

Y

L

M

binding glutamine: E128 (= E148), E183 (= E211), G235 (= G264), H239 (= H268), R292 (= R320), E298 (≠ Y326)
binding magnesium ion: E126 (= E146), E128 (= E148), E183 (= E211), E190 (= E218), H239 (= H268), E327 (= E355)
binding : F58 (≠ T74), R60 (≠ D79), G232 (= G261), N234 (≠ A263), G296 (≠ Q324), Y297 (≠ F325), R310 (= R338), Y367 (= Y395), Y421 (≠ E441), Q433 (≠ W453)

Sites not aligning to the query:

binding : 437

8s59O Glutamine synthetase
28% identity, 95% coverage: 22:455/458 of query aligns to 20:443/446 of 8s59O

query
sites
8s59O

E

D

V

V

L

K

Y
x
F

V

I

D
x
R

L

T

L

Q

I

F

A

T

D

D

M

T

N

L

G

G

V

I

R

K

G

S

K

W

R
x
A

I

I

E

P

R

A

T

E

S

Q

L

L

H

E

K

E

V

A

Y

F

E

E

K

N

G

G

I

V

N

M

L

F

P

D

A

G

S

S

L

-

F

-

A

-

L

-

D

S

I

I

N

Q

G

G

S

F

T

T

-

R

V

I

E

E

S

E

T

S

G

D

L

M

G

K

L

L

D

A

I

L

G

D

D

-

A

-

D

-

R

-

I

-

C

-

Y

-

P

-

I

-

P

P

D

S

T

T

L

F

C

R

N

I

E

L

P

P

W

W

Q

-

K

-

R
|
R

P

P

-

A

-

T

-

G

-

A

T
x
V

A

A

Q

R

L

I

L

L

M

G

T

D

M

V

H

Y

E

L

L

P

E

D

G

G

E

N

P

P

F

F

F

K

A

G

D

D

P

P

R

R

E

Y

V

V

L

L

R

K

Q

T

V

A

V

I

T

K

K

E

F

A

D

E

E

K

L

M

G

G

L

F

T

S

I

M

C

N

A

V

A

G

F

P

E

E

L

L

E

E

F

F

Y

F

L

L

I

F

D

-

Q

K

E

L

N

D

V

A

N

N

G

G

R

N

P

P

Q

T

P

T

P

E

R

L

S

T

P

D

I

Q

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

F

D

A

D

P

L

L

D

D

E

R

Y

A

A
x
Q

D

D

C

V

L
x
R

Q
x
R

D

D

I

I

L

D

E

Y

G

A

A

L

K

E

E

H

Q

M

G

G

I

F

P

Q

A

I

D

E

A

A

I
x
S

V

H

K

H

E

E

S

V

A

A

P

P

A

S

Q

Q

F

H

E

E

V

I

N

D

L

F

H

R

H

-

V

F

A

G

D

D

P

V

L

L

K

C

A

T

C

A

D

D

Y

N

A

V

V

V

L

T

L

F

K

K

R

Y

L

V

I

V

K

K

N

S

I

I

A

A

Y

Y

D

H

H

K

E

G

M

Y

D

Y

T

A

T

S

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A

N

G

G

N

S

G

G

L

M

H

H

V

S

H

N

I

Q

S

S

V

L

L

F

D

-

K

K

D

D

G

G

N

K

N

N

I

V

F

F

T

Y

S

-

E

-

D

D

P

P

E

D

Q

T

N

P

A

T

A

K

L

L

R

S

-

Q

-

D

-

A

-

M

H

Y

A

Y

V

I

G

G

V

L

L

L

E

K

T

H

L

I

P

R

A

E

S

F

M

T

A

A

F

V

L

T

C

N

P

P

N

V

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

Y

P

I

S

S

W

W

G

S

L

A

D

Q

N

N

R

R

T

S

V

S

A

L

L

I

R

R

V

I

P

P

T

A

G

T

S

R

A

G

D

N

A

G

V

T

R

R

I

I

E

E

H

L

R

R

V

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

A

A

L

V

M

L

L

A

R

G

A

V

G

H

L

H

E

G

G

L

I

T

K

N

N

K

K

I

I

E

D

P

P

G

G

A

E

P

P

I

T

E

N

G

V

N

N

S

I

Y

F

-

H

-

L

-

S

-

D

-

K

-

E

-

R

-

E

E

E

Q

R

L

G

E

I

Q

R

S

S

L

L

P

P

N

A

N

D

L

L

R

K

D

E

A

A

L

I

R

D

E

E

L

M

D

K

D

G

S

S

E

K

I

F

L

V

N

K

K

E

Y

A

I

L

D

G

P

E

K

H

Y

V

I

F

D

S

I

H

F

Y

V

L

A

C

C

A

K

K

E

E

S

M

E

E

L

W

E

D

E

E

F

Y

E

K

H

A

S

V

I

V

S

H

D

P

L

W

E

E

Y

L

N

S

W

R

Y

Y

L

L

binding 2-oxoglutaric acid: F23 (≠ Y25), R25 (≠ D27), A39 (≠ R41), R86 (= R102), V92 (≠ T104), Q168 (≠ A188), R171 (≠ L191), R172 (≠ Q192), S188 (≠ I208)

8tfbA Glutamine synthetase (see paper)
28% identity, 95% coverage: 22:455/458 of query aligns to 17:440/443 of 8tfbA

query
sites
8tfbA

E

D

V

V

L

K

Y

F

V

I

D

R

L

T

L

Q

I

F

A

T

D

D

M

T

N

L

G

G

V

I

R

K

G

S

K

W

R

A

I

I

E

P

R

A

T

E

S

Q

L

L

H

E

K

E

V

A

Y

F

E

E

K

N

G

G

I

V

N

M

L

F

P

D

A

G

S

S

L

-

F

-

A

-

L

-

D

S

I

I

N

Q

G

G

S

F

T

T

-

R

V

I

E

E

S

E

T

S

G

D

L

M

G

K

L

L

D

A

I

L

G

D

D

-

A

-

D

-

R

-

I

-

C

-

Y

-

P

-

I

-

P

P

D

S

T

T

L

F

C

R

N

I

E

L

P

P

W

W

Q

-

K

-

R

R

P

P

-

A

-

T

-

G

-

A

T

V

A

A

Q

R

L

I

L

L

M

G

T

D

M

V

H

Y

E

L

L

P

E

D

G

G

E

N

P

P

F

F

F

K

A

G

D

D

P

P

R

R

E

Y

V

V

L

L

R

K

Q

T

V

A

V

I

T

K

K

E

F

A

D

E

E

K

L

M

G

G

L

F

T

S

I

M

C

N

A

V

A

G

F

P

E

E

L

L

E
|
E

F

F

Y

F

L

L

I

F

D

-

Q

K

E

L

N

D

V

A

N

N

G

G

R

N

P

P

Q

T

P

T

P

E

R

L

S

T

P

D

I

Q

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

F

D

A

D

P

L

L

D

D

E

R

Y

A

A

Q

D

D

C

V

L

R

Q

R

D

D

I

I

L

D

E

Y

G

A

A

L

K

E

E

H

Q

M

G

G

I

F

P

Q

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

S

Q

Q

F

H

E
|
E

V

I

N

D

L

F

H

R

H

-

V

F

A

G

D

D

P

V

L

L

K

C

A

T

C

A

D

D

Y

N

A

V

V

V

L

T

L

F

K

K

R

Y

L

V

I

V

K

K

N

S

I

I

A

A

Y

Y

D

H

H

K

E

G

M

Y

D

Y

T

A

T

S

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A

N

G

G

N

S

G

G

L

M

H

H

V

S

H

N

I

Q

S

S

V

L

L

F

D

-

K

K

D

D

G

G

N

K

N

N

I

V

F

F

T

Y

S

-

E

-

D

D

P

P

E

D

Q

T

N

P

A

T

A

K

L

L

R

S

-

Q

-

D

-

A

-

M

H

Y

A

Y

V

I

G

G

V

L

L

L

E

K

T

H

L

I

P

R

A

E

S

F

M

T

A

A

F

V

L

T

C

N

P

P

N

V

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

G

V

A

P

Q

G

F

Y

Y

E

V

A

P

P

N

V

A

Y

P

I

S

S

W

W

G

S

L

A

D

Q

N

N

R

R

T

S

V

S

A

L

L

I

R

R

V

I

P

P

T

A

G

T

S

R

A

G

D

N

A

G

V

T

R

R

I

I

E

E

H

L

R

R

V

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

A

A

L

V

M

L

L

A

R

G

A

V

G

H

L

H

E

G

G

L

I

T

K

N

N

K

K

I

I

E

D

P

P

G

G

A

E

P

P

I

T

E

N

G

V

N

N

S

I

Y

F

-

H

-

L

-

S

-

D

-

K

-

E

-

R

-

E

E

E

Q

R

L

G

E

I

Q

R

S

S

L

L

P

P

N

A

N

D

L

L

R

K

D

E

A

A

L

I

R

D

E

E

L

M

D

K

D

G

S

S

E

K

I

F

L

V

N

K

K

E

Y

A

I

L

D

G

P

E

K

H

Y

V

I

F

D

S

I

H

F

Y

V

L

A

C

C

A

K

K

E

E

S

M

E

E

L

W

E

D

E

E

F

Y

E

K

H

A

S

V

I

V

S

H

D

P

L

W

E

E

Y

L

N

S

W

R

Y

Y

L

L

binding magnesium ion: E133 (= E148), E188 (= E211), E195 (= E218)

8tfkA Glutamine synthetase (see paper)
28% identity, 95% coverage: 22:455/458 of query aligns to 14:437/440 of 8tfkA

query
sites
8tfkA

E

D

V

V

L

K

Y

F

V

I

D

R

L

T

L

Q

I

F

A

T

D

D

M

T

N

L

G

G

V

I

R

K

G

S

K

W

R

A

I

I

E

P

R

A

T

E

S

Q

L

L

H

E

K

E

V

A

Y

F

E

E

K

N

G

G

I

V

N

M

L

F

P

D

A

G

S

S

L

-

F

-

A

-

L

-

D

S

I

I

N

Q

G

G

S

F

T

T

-

R

V

I

E

E

S

E

T

S

G

D

L

M

G

K

L

L

D

A

I

L

G

D

D

-

A

-

D

-

R

-

I

-

C

-

Y

-

P

-

I

-

P

P

D

S

T

T

L

F

C

R

N

I

E

L

P

P

W

W

Q

-

K

-

R

R

P

P

-

A

-

T

-

G

-

A

T

V

A

A

Q

R

L

I

L

L

M

G

T

D

M

V

H

Y

E

L

L

P

E

D

G

G

E

N

P

P

F

F

F

K

A

G

D

D

P

P

R

R

E

Y

V

V

L

L

R

K

Q

T

V

A

V

I

T

K

K

E

F

A

D

E

E

K

L

M

G

G

L

F

T

S

I

M

C

N

A

V

A

G

F

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

I

F

D

-

Q

K

E

L

N

D

V

A

N

N

G

G

R

N

P

P

Q

T

P

T

P

E

R

L

S

T

P

D

I

Q

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

F

D

A

D

P

L

L

D

D

E

R

Y

A

A

Q

D

D

C

V

L

R

Q

R

D

D

I

I

L

D

E

Y

G

A

A

L

K

E

E

H

Q

M

G

G

I

F

P

Q

A

I

D

E

A

A

I

S

V

H

K

H

E
|
E

S

V

A

A

P

P

A

S

Q

Q

F

H

E
|
E

V

I

N
x
D

L
x
F

H

R

H

-

V
x
F

A

G

D

D

P

V

L

L

K

C

A

T

C

A

D

D

Y

N

A

V

V

V

L

T

L

F

K

K

R

Y

L

V

I

V

K

K

N

S

I

I

A

A

Y

Y

D

H

H

K

E

G

M

Y

D

Y

T

A

T

S

F

F

M

M

A

P

K

K

P

P

Y

L

P

F

G

G

Q

V

A

N

G
|
G

N

S

G

G

L

M

H
|
H

V

S

H
x
N

I

Q

S

S

V

L

L

F

D

-

K

K

D

D

G

G

N

K

N

N

I

V

F

F

T

Y

S

-

E

-

D

D

P

P

E

D

Q

T

N

P

A

T

A

K

L

L

R

S

-

Q

-

D

-

A

-

M

H

Y

A

Y

V

I

G

G

V

L

L

L

E

K

T

H

L

I

P

R

A

E

S

F

M

T

A

A

F

V

L

T

C

N

P

P

N

V

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

G

V

A

P

Q

G

F

Y

Y
x
E

V

A

P

P

N

V

A

Y

P

I

S

S

W

W

G

S

L

A

D

Q

N

N

R
|
R

T

S

V

S

A

L

L

I

R
|
R

V

I

P

P

T

A

G

T

S

R

A

G

D

N

A
x
G

V

T

R
|
R

I

I

E
|
E

H

L

R
|
R

V

C

A

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

L

L

M

A

M

F

A

A

A

L

V

M

L

L

A

R

G

A

V

G

H

L

H

E

G

G

L

I

T

K

N

N

K

K

I

I

E

D

P

P

G

G

A

E

P

P

I

T

E

N

G

V

N

N

S

I

Y

F

-

H

-

L

-

S

-

D

-

K

-

E

-

R

-

E

E

E

Q

R

L

G

E

I

Q

R

S

S

L

L

P

P

N

A

N

D

L

L

R

K

D

E

A

A

L

I

R

D

E

E

L

M

D

K

D

G

S

S

E

K

I

F

L

V

N

K

K

E

Y

A

I

L

D

G

P

E

K

H

Y

V

I

F

D

S

I

H

F

Y

V

L

A

C

C

A

K

K

E

E

S

M

E

E

L

W

E

D

E

E

F

Y

E

K

H

A

S

V

I

V

S

H

D

P

L

W

E

E

Y

L

N

S

W

R

Y

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E146), D194 (≠ N220), F195 (≠ L221), F197 (≠ V224), N243 (≠ H270), R312 (= R338), R317 (= R343), G325 (≠ A351), R327 (= R353)
binding magnesium ion: E128 (= E146), E128 (= E146), E130 (= E148), E185 (= E211), E192 (= E218), E192 (= E218), H241 (= H268), E329 (= E355)
binding l-methionine-s-sulfoximine phosphate: E128 (= E146), E130 (= E148), E185 (= E211), E192 (= E218), G237 (= G264), H241 (= H268), R294 (= R320), E300 (≠ Y326), R312 (= R338), R331 (= R357)

7tdvC Glutamine synthetase (see paper)
27% identity, 95% coverage: 19:455/458 of query aligns to 13:440/443 of 7tdvC

query
sites
7tdvC

E

E

H

E

P

E

E

N

V

V

L

R

Y

Y

V

L

D

R

L

L

L

Q

I

F

A

T

D

D

M

I

N

L

G

G

V

T

V

I

R

K

G

N

K

V

R

E

I

V

E

P

R

V

T

S

S

Q

L

L

H

E

K

K

V

V

Y

L

E

D

K

N

G

-

I

-

N

-

L

-

P

-

A

-

S

-

L

-

F

-

A

E

L

M

D

M

I

F

N

D

G

G

S

S

T

S

V

I

E

E

S

G

T

F

G

-

L

-

G

-

L

V

D

R

I

I

G

E

D

E

A

S

D

D

R

M

I

Y

C

L

Y

H

P

P

I

D

P

L

D

D

T

T

L

W

C

V

N

I

E

F

P

P

W

W

Q

T

-

A

-

G

K

Q

R

G

P

K

T

V

A

A

Q

R

L

L

L

I

M

C

T

D

M

V

H

Y

E

K

L

T

E

D

G

G

E

T

P

P

F

F

F

E

A

G

D

D

P

P

R

R

E

A

V

N

L

L

R

K

Q

R

V

V

V

L

T

K

K

E

F

M

D

E

E

D

L

L

G

G

L

F

T

T

-

D

I

F

C

N

A

L

A
x
G

F

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

I

F

D

K

Q

L

E

D

N

E

V

-

N

K

G

G

R

E

P

P

Q

T

P

L

P

E

R

L

S

N

P

D

I

D

S

G

G

G

K

-

R

-

P

-

Q

-

S

-

T

-

Q

-

V

Y

Y

F

L

D

I

L

D

A

D

P

L

T

D

D

E

L

Y

G

A

E

D

N

C

C

L

R

Q

R

D

D

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binding adenosine-5'-diphosphate: G129 (≠ A144), E131 (= E146), E183 (≠ D206), D197 (≠ N220), F198 (≠ L221), K199 (≠ H222), Y200 (≠ H223), N246 (≠ H270), V247 (≠ I271), S248 (= S272), R320 (= R343), S328 (≠ A351), R330 (= R353)
binding magnesium ion: E131 (= E146), E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), E195 (= E218), H244 (= H268), E332 (= E355)
binding l-methionine-s-sulfoximine phosphate: E131 (= E146), E133 (= E148), E188 (= E211), E195 (= E218), G240 (= G264), H244 (= H268), R297 (= R320), E303 (≠ Y326), R315 (= R338)

Query Sequence

>PP_5184 FitnessBrowser__Putida:PP_5184
MSVPPRAVQLNEANAFLKEHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPAS
LFALDINGSTVESTGLGLDIGDADRICYPIPDTLCNEPWQKRPTAQLLMTMHELEGEPFF
ADPREVLRQVVTKFDELGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPQSTQVYL
IDDLDEYADCLQDILEGAKEQGIPADAIVKESAPAQFEVNLHHVADPLKACDYAVLLKRL
IKNIAYDHEMDTTFMAKPYPGQAGNGLHVHISVLDKDGNNIFTSEDPEQNAALRHAVGGV
LETLPASMAFLCPNVNSYRRFGAQFYVPNAPSWGLDNRTVALRVPTGSADAVRIEHRVAG
ADANPYLMMAAVLAGVHHGLTNKIEPGAPIEGNSYEQLEQSLPNNLRDALRELDDSEILN
KYIDPKYIDIFVACKESELEEFEHSISDLEYNWYLHTV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory