SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_5289 PP_5289 Acetylglutamate kinase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTN2 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
93% identity, 99% coverage: 1:299/301 of query aligns to 1:299/301 of Q9HTN2

query
sites
Q9HTN2

M
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M

T

T

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L

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R

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D

A

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Q

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D

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A

A

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G

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K

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E

A

A

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L

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K

A

A

E

E

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N

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A

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K

M1 (= M1) modified: Initiator methionine, Removed
R90 (= R90) binding
N195 (= N195) binding

2bufC Arginine feed-back inhibitable acetylglutamate kinase (see paper)
93% identity, 99% coverage: 2:299/301 of query aligns to 1:298/298 of 2bufC

query
sites
2bufC

T

T

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L

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D

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T

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I
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D

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K

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E

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N

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Y

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K

active site: K32 (= K33), G35 (= G36), G68 (= G69), D198 (= D199), K254 (= K255)
binding adenosine-5'-diphosphate: N36 (= N37), T217 (= T218), N218 (= N219), I219 (= I220), L222 (= L223), M223 (= M224), T246 (= T247), I247 (= I248), Y248 (= Y249), G250 (= G251), M251 (= M252), K254 (= K255)

2bufA Arginine feed-back inhibitable acetylglutamate kinase (see paper)
90% identity, 99% coverage: 2:299/301 of query aligns to 1:290/292 of 2bufA

query
sites
2bufA

T

T

L

L

D

S

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R

D

D

A

D

A

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Y

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F

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G

K

K

T

T

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I

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K

Y

Y

G

G

G
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G

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N

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A

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M

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S

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E

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L

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R

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N

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D

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L

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L

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T

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N

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A

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G

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M

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D

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K

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E

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N

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E

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A

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C

A

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N

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S

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T

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N

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R

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K

active site: K32 (= K33), G35 (= G36), G68 (= G69), D190 (= D199), K246 (= K255)
binding n-acetyl-l-glutamate: G66 (= G67), G67 (= G68), I71 (= I72), M88 (= M89), R89 (= R90), N186 (= N195), A189 (= A198)

2bufK Arginine feed-back inhibitable acetylglutamate kinase (see paper)
85% identity, 98% coverage: 2:297/301 of query aligns to 1:273/273 of 2bufK

query
sites
2bufK

T

T

L

L

D

S

R

R

D

D

A

D

A

A

S

A

H

Q

V

V

A

A

E

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L

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E

A

A

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L

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P

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Y

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I

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R

F

F

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V

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G

K

K

T

T

L

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V

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K

Y

Y

G

G

G
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G

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N

A

A

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M

E

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L

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K

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K

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A

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V

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I

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G

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D

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L

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K

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I

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E

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S

H

H

F

-

I

-

D

-

G

-

M

-

R
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R

V

V

T

T

D

D

S

A

A

A

T

T

M

M

D

D

V

V

V
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V

E

E

M

M

V

V

L

L

G

G

Q

Q

V

V

N

N

K

K

D

D

I

I

V

V

N

N

L

L

I

I

N

N

R

R

H

H

G

G

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S

A

A

I

I

G

G

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L

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T

G

G

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K

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D

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A

E

E

L

L

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A

A

R

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K

L

L

T

T

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S

-

R

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-

E

-

M

-

T

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P

-

E

-

I

-

D

-

I

-

G

-

H

-

V

-

G

G

E

E

V

V

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T

S

G

V

V

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N

T

V

D

G

L

L

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N

M

M

L

L

V

V

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K

G

G

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F

F

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V

V

I

I

A

A

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I

I

G

G

V

V

G

G

A

S

N

N

G

G

E

E

S

S

Y

Y

N
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N

I
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I

N
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N

A
|
A

D
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D

L

L

V

V

A

A

G

G

K

K

V

V

A

A

E

E

A

A

L

L

K

K

A

A

E

E

K

K

L

L

M

M

L

L

T

T

N

N

I

I

A

A

G

G

L

L

M

M

D

D

K

K

Q

Q

G

G

Q

Q

V

V

L

L

T

T

G

G

L

L

T

S

T

T

E

E

Q

Q

V

V

N

N

E

E

L

L

I

I

A

A

D

D

G

G

T

T

I

I

Y

Y

G

G

M

M

L

L

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K
|
K

I

I

K

R

C

C

A

A

L

L

D

E

A

A

V

V

Q

Q

G

G

V

V

N

T

S

S

S

A

H

H

I

I

D

D

G

G

R

R

V

V

P

P

N

N

A

A

V

V

L

L

E

E

I

I

F

F

T

T

D

D

S

S

G

G

V

V

G

G

T

T

L

L

I

I

T

S

N

N

active site: K32 (= K33), G35 (= G36), G68 (= G69), D175 (= D199), K231 (= K255)
binding n-acetyl-l-glutamate: R84 (= R90), V94 (= V100), N171 (= N195), I172 (= I196), N173 (= N197), A174 (= A198)

2btyA Acetylglutamate kinase from thermotoga maritima complexed with its inhibitor arginine (see paper)
52% identity, 94% coverage: 14:295/301 of query aligns to 8:277/282 of 2btyA

query
sites
2btyA

V

V

L

L

S

L

E

E

A

A

L

L

P

P

Y
|
Y

I

I

R

K

R

E

F
|
F

V

Y

G

G

K

K

T

T

L

F

V

V

I

I

K
|
K

Y

F

G
|
G

N

S

A

A

M

M

E

K

S

Q

E

E

E

N

L

A

K

K

T

K

G

A

F

F

A

I

R

Q

D

D

I

I

V

I

L

L

M

L

K

K

A

Y

V

T

G

G

I

I

N

K

P

P

V

I

V

V

H

H

G
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G

P

P

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A

I

I

G

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D

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L

M

K

K

R

D

L

L

S

G

I

I

E

E

S

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H

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F

F

I

K

D

N

G

G

M

H

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R

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V

T

T

D

D

S

E

A

K

T

T

M

M

D

E

V

I

V

V

E

E

M

M

V

V

L

L

G

V

G

G

Q

K

V

I

N

N

K

K

D

E

I

I

V

V

N

M

L

N

I

L

N

N

R

L

H

H

G

G

S

R

A

A

I

V

G

G

L

I

T

C

G

G

K

K

D

D

A

S

E

K

L

L

I

I

R

V

A

A

R

E

K

K

L

E

T

T

V

K

S

H

R

G

Q

-

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

Y

V

V

G

G

E

K

V

V

V

K

S

K

V

V

N

N

T

P

D

E

L

I

L

L

N

H

M

A

L

L

V

I

K

E

G

N

D

D

F

Y

I

I

P

P

V

V

I

I

A

A

P

P

I

V

G

G

V

I

G

G

A

E

N

D

G

G

E

H

S

S

Y

Y

N

N

I

I

N
|
N

A

A

D
|
D

L

T

V

A

A

A

G

A

K

E

V

I

A

A

E

K

A

S

L

L

K

M

A

A

E

E

K
|
K

L

L

M

I

L

L

T

T

N

D

I

V

A

D

G

G

L

V

M

L

D

-

K

K

Q

D

G

G

Q

K

V

L

L

I

T
x
S

G

T

L

L

T

T

T

P

E

D

Q

E

V

A

N

E

E

E

L

L

I

I

A

R

D

D

G

G

T

T

I

V

Y

T

G

G

M

M

L

I

P

P

K
|
K

I

V

K

E

C

C

A

A

L

V

D

S

A

A

V

V

Q

R

G

G

V

V

N

G

S

A

S

V

H
|
H

I

I

D

N

G

G

R

G

V

L

P

E

N

H

A

A

V

I

L

L

E
|
E

I
|
I

F

F

T
x
S

D
x
R

S
x
K

G
|
G

V

I

G
|
G

T
|
T

L
x
M

I

I

active site: K27 (= K33), G30 (= G36), G63 (= G69), D182 (= D199), K237 (= K255)
binding arginine: Y15 (= Y21), F19 (= F25), K196 (= K213), S214 (≠ T232), H253 (= H271), E266 (= E284), I267 (= I285), S269 (≠ T287), R270 (≠ D288), K271 (≠ S289), G272 (= G290), G274 (= G292), T275 (= T293), M276 (≠ L294)
binding n-acetyl-l-glutamate: K27 (= K33), G29 (= G35), G30 (= G36), G61 (= G67), G62 (= G68), G63 (= G69), N180 (= N197), D182 (= D199)

Q9X2A4 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
52% identity, 94% coverage: 14:295/301 of query aligns to 8:277/282 of Q9X2A4

query
sites
Q9X2A4

V

V

L

L

S

L

E

E

A

A

L

L

P

P

Y

Y

I

I

R

K

R

E

F

F

V

Y

G

G

K

K

T

T

L

F

V

V

I

I

K

K

Y

F

G

G

N

S

A

A

M

M

E

K

S

Q

E

E

E

N

L

A

K

K

T

K

G

A

F

F

A

I

R

Q

D

D

I

I

V

I

L

L

M

L

K

K

A

Y

V

T

G

G

I

I

N

K

P

P

V

I

V

V

H

H

G

G

P

P

Q

A

I

I

G

S

D

Q

L

M

K

K

R

D

L

L

S

G

I

I

E

E

S

P

H

V

F

F

I

K

D

N

G

G

M

H

R

R

V

V

T

T

D

D

S

E

A

K

T

T

M

M

D

E

V

I

V

V

E

E

M

M

V

V

L

L

G

V

G

G

Q

K

V

I

N

N

K

K

D

E

I

I

V

V

N

M

L

N

I

L

N

N

R

L

H

H

G

G

S

R

A

A

I

V

G

G

L

I

T

C

G

G

K

K

D

D

A

S

E

K

L

L

I

I

R

V

A

A

R

E

K

K

L

E

T

T

V

K

S

H

R

G

Q

-

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

Y

V

V

G

G

E

K

V

V

V

K

S

K

V

V

N

N

T

P

D

E

L

I

L

L

N

H

M

A

L

L

V

I

K

E

G

N

D

D

F

Y

I

I

P

P

V

V

I

I

A

A

P

P

I

V

G

G

V

I

G

G

A

E

N

D

G

G

E

H

S

S

Y

Y

N
|
N

I

I

N

N

A

A

D

D

L

T

V

A

A

A

G

A

K

E

V

I

A

A

E

K

A

S

L

L

K

M

A

A

E

E

K
|
K

L

L

M

I

L

L

T

T

N

D

I

V

A

D

G

G

L

V

M

L

D

-

K

K

Q

D

G

G

Q

K

V

L

L

I

T
x
S

G

T

L

L

T

T

T

P

E

D

Q

E

V

A

N

E

E

E

L

L

I

I

A

R

D

D

G

G

T

T

I

V

Y

T

G

G

M

M

L

I

P

P

K

K

I

V

K

E

C

C

A

A

L

V

D

S

A

A

V

V

Q

R

G

G

V

V

N

G

S

A

S

V

H

H

I

I

D

N

G

G

R

G

V

L

P

E

N

H

A

A

V

I

L

L

E
|
E

I
|
I

F
|
F

T
x
S

D

R

S

K

G

G

V

I

G

G

T

T

L

M

I

I

N178 (= N195) binding
K196 (= K213) binding
S214 (≠ T232) binding
EIFS 266:269 (≠ EIFT 284:287) binding

2v5hB Controlling the storage of nitrogen as arginine: the complex of pii and acetylglutamate kinase from synechococcus elongatus pcc 7942 (see paper)
49% identity, 96% coverage: 8:295/301 of query aligns to 3:281/289 of 2v5hB

query
sites
2v5hB

A

A

S

A

H

D

V

R

A

V

E

R

V

I

L

L

S

S

E

E

A

A

L

L

P

P

Y

Y

I

L

R

Q

F

F

V

A

G

G

K

R

T

T

L

V

V

V

I

V

K
|
K

Y

Y

G

G

G
|
G

N

A

A

A

M

M

E

K

S

Q

E

E

L

L

K

K

T

E

G

A

F

V

A

M

R

R

D

D

I

I

V

V

L

F

M

L

K

A

A

C

V

V

G

G

I

M

N

R

P

P

V

V

H

H

G
|
G

P

P

Q

E

I
|
I

G

N

D

A

L

W

L

L

K

G

R

R

L

V

S

G

I

I

E

E

S

P

H

Q

F

F

I

H

D

N

G

G

M
x
L

R
|
R

V

V

T

T

D

D

S

A

A

D

T

T

M

M

D

E

V

V

E

E

M

M

V

V

L

L

G

V

G

G

Q

R

V

V

N

N

K

K

D

D

I

I

V

V

N

S

L

R

I

I

N

N

R

T

H

T

G

G

S

R

A

A

I

V

G

G

L

F

T

C

G

G

K

T

D

D

A

G

E

R

L

L

I

V

R

L

A

A

R

R

K

P

L

H

T

D

V

Q

S

E

R

-

Q

-

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

G

I

I

G

G

H

F

V

V

G

G

E

E

V

V

V

N

S

S

V

V

N

N

T

S

D

E

L

V

L

I

N

E

M

P

L

L

V

L

K

E

G

R

D

G

F

Y

I

I

P

P

V

V

I

I

A

S

P

S

I

V

G

A

V

A

G

D

A

E

N

N

G

G

E

Q

S

S

Y

F

N
|
N

I
|
I

N
|
N

A
|
A

D
|
D

L

T

V

V

A

A

G

G

K

E

V

I

A

A

E

A

A

A

L

L

K

N

A

A

E

E

K

K

L

L

M

I

L

L

T

T

N

D

I

T

A

R

G

G

L

I

M

L

D

E

-

D

-

P

-

K

K

R

Q

P

G

E

Q

S

V

L

L

I

T

P

G

R

L

L

T

N

T

I

E

P

Q

Q

V

S

N

R

E

E

L

L

I

I

A

A

D

Q

G

G

T

I

I

V

Y

G

G

G

M

M

L

I

P

P

K
|
K

I

V

K

D

C

C

A

C

L

I

D

R

A

S

V

L

Q

A

G

Q

G

G

V

V

N

R

S

A

H

H

I

I

D

D

G

G

R

R

V

I

P

P

N

H

A

A

V

L

L

L

E

E

I

I

F

F

T

T

D

D

S

A

G

G

V

I

G

G

T

T

L

M

I

I

active site: K28 (= K33), G31 (= G36), G64 (= G69), D182 (= D199), K241 (= K255)
binding n-acetyl-l-glutamate: G62 (= G67), G63 (= G68), G64 (= G69), I67 (= I72), L84 (≠ M89), R85 (= R90), N178 (= N195), I179 (= I196), N180 (= N197), A181 (= A198)

Q9SCL7 Acetylglutamate kinase, chloroplastic; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AtNAGK; EC 2.7.2.8 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
47% identity, 94% coverage: 13:296/301 of query aligns to 71:346/347 of Q9SCL7

query
sites
Q9SCL7

E

E

V

I

L

L

S

S

E

E

A

S

L

L

P

P

Y

F

I

I

R

Q

R

K

F

F

V

R

G

G

K

K

T

T

L

I

V

V

I

V

K

K

Y

Y

G

G

G
|
G

N
x
A

A

A

M

M

E

T

S

S

E

P

E

E

L

L

K

K

T

S

G

S

F

V

A

V

R

S

D

D

I

L

V

V

L

L

M

L

K

A

A

C

V

V

G

G

I

L

N

R

P

P

V

I

V

L

V

V

H

H

G

G

G
|
G

G

G

P

P

Q

D

I

I

G

N

D

R

L

Y

L

L

K

K

R

Q

L

L

S

N

I

I

E

P

S

A

H

E

F

F

I

R

D

D

G

G

M

L

R
|
R

V

V

T

T

D

D

S

A

A

T

T

T

M

M

D

E

V

I

V

V

E

S

M

M

V

V

L

L

G

V

G

G

Q

K

V

V

N

N

K

K

D

N

I

L

V

V

N

S

L

L

I

I

N

N

R

A

H

A

G

G

G

A

S

T

A

A

I

V

G

G

L

L

T

S

G

G

K

H

D

D

A

G

E

R

L

L

I

L

R

T

A

A

R

R

K

P

L

V

T

P

V

N

S

S

R

A

Q

Q

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

-

I

L

G

G

H

F

V

V

G

G

E

E

V

V

V

A

S

R

V

V

N

D

T

P

D

S

L

V

L

L

N

R

M

P

L

L

V

V

K

D

G

Y

D

G

F

Y

I

I

P

P

V

V

I

I

A

A

P

S

I

V

G

A

V

A

G

D

A

D

N

S

G

G

E

Q

S

A

Y

Y

N
|
N

I
|
I

N
|
N

A
|
A

D

D

L

T

V

V

A

A

G

G

K

E

V

L

A

A

E

A

A

A

L

L

K

G

A

A

E

E

K
|
K

L

L

M

I

L

L

T
|
T

N
x
D

I

V

A

A

G

G

L

I

M
x
L

D

E

K

N

Q

K

-

E

-

D

-

P

G

S

Q

S

V

L

L

I

T
x
K

G

E

L

I

T

D

T

I

E

K

Q

G

V

V

N

K

E

K

L

M

I

I

A

E

D

D

G

G

T
x
K

I
x
V

Y
x
A

G
|
G

M
|
M

L
x
I

P
|
P

K
|
K

I

V

K

K

C

C

A

C

L

I

D

R

A

S

V

L

Q

A

G

Q

G

G

V

V

N

K

S

T

S

A

H

S

I

I

D

D

G

G

R

R

V

R

P

Q

N

H

A

S

V

L

L

L

L

H

E
|
E

I
|
I

F
x
M

T
x
S

D

D

S

E

G

G

V

A

G
|
G

T

T

L

M

I

I

T

T

GA 94:95 (≠ GN 36:37) binding
G126 (= G68) binding
R148 (= R90) binding
NINA 242:245 (= NINA 195:198) binding
K260 (= K213) binding
TD 265:266 (≠ TN 218:219) binding
L271 (≠ M224) binding
K282 (≠ T232) binding
297:305 (vs. 247:255, 56% identical) binding
EIMS 334:337 (≠ EIFT 284:287) binding
G342 (= G292) binding

Sites not aligning to the query:

1:50 modified: transit peptide, Chloroplast

4usjB N-acetylglutamate kinase from arabidopsis thaliana in complex with pii from chlamydomonas reinhardtii (see paper)
47% identity, 94% coverage: 13:296/301 of query aligns to 5:280/281 of 4usjB

query
sites
4usjB

E

E

V

I

L

L

S

S

E

E

A

S

L

L

P

P

Y

F

I

I

R

Q

R

K

F
|
F

V

R

G

G

K

K

T

T

L

I

V

V

I

V

K
|
K

Y

Y

G
|
G

N

A

A

A

M

M

E

T

S

S

E

P

E

E

L

L

K

K

T

S

G

S

F

V

A

V

R

S

D

D

I

L

V

V

L

L

M

L

K

A

A

C

V

V

G

G

I

L

N

R

P

P

V

I

V

L

V

V

H

H

G
|
G

P

P

Q

D

I
|
I

G

N

D

R

L

Y

L

L

K

K

R

Q

L

L

S

N

I

I

E

P

S

A

H

E

F

F

I

R

D

D

G

G

M
x
L

R
|
R

V

V

T

T

D

D

S

A

A

T

T

T

M

M

D

E

V

I

V

V

E

S

M

M

V

V

L

L

G

V

G

G

Q

K

V

V

N

N

K

K

D

N

I

L

V

V

N

S

L

L

I

I

N

N

R

A

H

A

G

G

G

A

S

T

A

A

I

V

G

G

L

L

T

S

G

G

K

H

D

D

A

G

E

R

L

L

I

L

R

T

A

A

R

R

K

P

L

V

T

P

V

N

S

S

R

A

Q

Q

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

-

I

L

G

G

H

F

V
|
V

G

G

E

E

V

V

V

A

S

R

V

V

N

D

T

P

D

S

L

V

L

L

N

R

M

P

L

L

V

V

K

D

G

Y

D

G

F

Y

I

I

P

P

V

V

I

I

A

A

P

S

I

V

G

A

V

A

G

D

A

D

N

S

G

G

E

Q

S

A

Y

Y

N
|
N

I
|
I

N
|
N

A
|
A

D
|
D

L

T

V

V

A

A

G

G

K

E

V

L

A

A

E

A

A

A

L

L

K

G

A

A

E

E

K
|
K

L

L

M

I

L

L

T

T

N

D

I

V

A

A

G

G

L

I

M

L

D

E

K

N

Q

K

-

E

-

D

-

P

G

S

Q

S

V

L

L

I

T
x
K

G

E

L

I

T

D

T

I

E

K

Q

G

V

V

N

K

E

K

L

M

I

I

A

E

D

D

G

G

T

K

I

V

Y

A

G

G

M

M

L

I

P

P

K
|
K

I

V

K

K

C

C

A

C

L

I

D

R

A

S

V

L

Q

A

G

Q

G

G

V

V

N

K

S

T

S

A

H

S

I

I

D

D

G

G

R

R

V

R

P

Q

N

H

A

S

V

L

L

L

L

H

E
|
E

I
|
I

F

M

T
x
S

D
|
D

S
x
E

G
|
G

V

A

G
|
G

T

T

L
x
M

I

I

T

T

active site: K25 (= K33), G28 (= G36), G61 (= G69), D180 (= D199), K239 (= K255)
binding arginine: F17 (= F25), K194 (= K213), K216 (≠ T232), E268 (= E284), I269 (= I285), S271 (≠ T287), D272 (= D288), E273 (≠ S289), G274 (= G290), G276 (= G292), M278 (≠ L294)
binding n-acetyl-l-glutamyl 5-phosphate: K25 (= K33), G27 (= G35), G28 (= G36), G59 (= G67), G60 (= G68), G61 (= G69), I64 (= I72), L81 (≠ M89), R82 (= R90), V140 (= V159), N176 (= N195), I177 (= I196), N178 (= N197), A179 (= A198)

2rd5B Structural basis for the regulation of n-acetylglutamate kinase by pii in arabidopsis thaliana (see paper)
47% identity, 94% coverage: 13:296/301 of query aligns to 7:282/283 of 2rd5B

query
sites
2rd5B

E

E

V

I

L

L

S

S

E

E

A

S

L

L

P

P

Y

F

I

I

R

Q

R

K

F
|
F

V

R

G

G

K

K

T

T

L

I

V

V

I

V

K
|
K

Y

Y

G
|
G

N
x
A

A

A

M

M

E

T

S

S

E

P

E

E

L

L

K

K

T

S

G

S

F

V

A

V

R

S

D

D

I

L

V

V

L

L

M

L

K

A

A

C

V

V

G

G

I

L

N

R

P

P

V

I

V

L

V

V

H

H

G
|
G

P

P

Q

D

I
|
I

G

N

D

R

L

Y

L

L

K

K

R

Q

L

L

S

N

I

I

E

P

S

A

H

E

F

F

I

R

D

D

G

G

M
x
L

R
|
R

V

V

T

T

D

D

S

A

A

T

T

T

M

M

D

E

V

I

V

V

E

S

M

M

V

V

L
|
L

G

V

G

G

Q

K

V

V

N

N

K

K

D

N

I

L

V

V

N

S

L

L

I

I

N

N

R

A

H

A

G

G

G

A

S

T

A

A

I

V

G

G

L

L

T

S

G

G

K

H

D

D

A

G

E

R

L

L

I

L

R

T

A

A

R

R

K

P

L

V

T

P

V

N

S

S

R

A

Q

Q

T

-

P

-

E

-

M

-

T

-

P

-

E

-

I

-

D

-

I

L

G

G

H

F

V

V

G

G

E

E

V

V

V

A

S

R

V

V

N

D

T

P

D

S

L

V

L

L

N

R

M

P

L

L

V

V

K

D

G

Y

D

G

F

Y

I

I

P

P

V

V

I

I

A

A

P

S

I

V

G

A

V

A

G

D

A

D

N

S

G

G

E

Q

S

A

Y

Y

N
|
N

I
|
I

N
|
N

A
|
A

D
|
D

L

T

V

V

A

A

G

G

K

E

V

L

A

A

E

A

A

A

L

L

K

G

A

A

E

E

K
|
K

L

L

M

I

L

L

T
|
T

N
x
D

I
x
V

A

A

G
|
G

L
x
I

M
x
L

D

E

K

N

Q
x
K

-

E

-

D

-

P

G

S

Q

S

V

L

L

I

T
x
K

G

E

L

I

T

D

T

I

E

K

Q

G

V

V

N

K

E

K

L

M

I

I

A

E

D

D

G

G

T
x
K

I
x
V

Y
x
A

G

G

G
|
G

M
|
M

L

I

P

P

K
|
K

I

V

K

K

C

C

A

C

L

I

D

R

A

S

V

L

Q

A

G

Q

G

G

V

V

N

K

S

T

S

A

H

S

I

I

D

D

G

G

R

R

V

R

P

Q

N

H

A

S

V

L

L

L

L

H

E
|
E

I
|
I

F

M

T
x
S

D
|
D

S
x
E

G
|
G

V

A

G
|
G

T

T

L
x
M

I

I

T

T

active site: K27 (= K33), G30 (= G36), G63 (= G69), D182 (= D199), K241 (= K255)
binding adenosine-5'-diphosphate: K27 (= K33), G29 (= G35), G30 (= G36), A31 (≠ N37), T201 (= T218), D202 (≠ N219), V203 (≠ I220), G205 (= G222), I206 (≠ L223), L207 (≠ M224), K210 (≠ Q227), K233 (≠ T247), V234 (≠ I248), A235 (≠ Y249), G237 (= G251), M238 (= M252), K241 (= K255)
binding arginine: F19 (= F25), K196 (= K213), K218 (≠ T232), E270 (= E284), I271 (= I285), S273 (≠ T287), D274 (= D288), E275 (≠ S289), G276 (= G290), G278 (= G292), M280 (≠ L294)
binding n-acetyl-l-glutamate: G61 (= G67), G62 (= G68), G63 (= G69), I66 (= I72), L83 (≠ M89), R84 (= R90), L98 (= L104), N178 (= N195), I179 (= I196), N180 (= N197), A181 (= A198)

7nlxA Crystal structure of mycobacterium tuberculosis argb in complex with 7-(trifluoromethyl)quinolin-4-ol.
47% identity, 95% coverage: 9:293/301 of query aligns to 5:285/290 of 7nlxA

query
sites
7nlxA

S

T

H

H

V

I

-

K

A

A

E

Q

V

V

L

L

S

A

E

E

A

A

L

L

P

P

Y

W

I

L

R

K

R

Q

F

L

V

H

G

G

K

K

T

V

L

V

V

V

I

V

K

K

Y

Y

G

G

N

N

A

A

M

M

E

T

S

D

E

D

E

T

L

L

K

R

T

R

G

A

F

F

A

A

R

A

D

D

I

M

V

A

L

F

M

L

K

R

A

N

V

C

G

G

I

I

N

H

P

P

V

V

H

H

G

G

P

P

Q

Q

I

I

G

T

D

A

L

M

L

L

K

R

R

R

L

L

S

G

I

I

E

E

S

G

H

D

F

F

I

K

D

G

G

G

M

F

R

R

V

V

T

T

D

T

S

P

A

E

T

V

M

L

D

D

V

V

V

A

E

R

M

M

V

V

L

L

G

F

G

G

Q

Q

V

V

N

G

K

R

D

E

I

L

V

V

N

N

L

L

I

I

N

N

R

A

H

H

G

G

G

P

S

Y

A

A

I
x
V

G

G

L
x
I

T

T

G

G

K

E

D
|
D

A
|
A

E

Q

L
|
L

I

F

R

T

A

A

R

V

K

R

L

R

T

S

V

V

S

T

R

V

Q

D

T

G

P

V

E

A

M

T

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

L

V

V

G

G

E

D

V

V

V

D

S

Q

V

V

N

N

T

T

D

A

L

A

L

M

N

L

M

D

L
|
L

V

V

K

A

G

A

D

G

F

R

I

I

P

P

V

V

I

V

A

S

P

T

I

L

G

A

V

P

G

D

A

A

N

D

G

G

E

V

S

V

Y

H

N

N

I

I

N

N

A

A

D

D

L

T

V

A

A

A

G

A

K

A

V

V

A

A

E

E

A

A

L

L

K

G

A

A

E

E

K

K

L

L

M

L

L

M

L

L

T

T

N

D

I

I

A

D

G

G

L

L

M

Y

D

T

K

R

-

W

-

P

-

D

Q

R

G

D

Q

S

V

L

L

V

T

S

G

E

L

I

T

D

T

T

E

G

Q

T

V

L

N

A

E

Q

L

L

I

L

A

P

D

-

G

-

T

T

I

L

Y

E

G

S

G

G

M

M

L

V

P

P

K

K

I

V

K

E

C

A

A

C

L

L

D

R

A

A

V

V

Q

I

G

G

V

V

N

P

S

S

S

A

H

H

I

I

D

D

G

G

R

R

V

V

P

T

N

H

A

C

V

V

L

L

L

V

E

E

I

L

F

F

T

T

D

D

S

A

G

G

V

T

G

G

T

T

binding 7-(trifluoromethyl)quinolin-4-ol: V121 (≠ I124), I123 (≠ L126), D127 (= D130), A128 (= A131), L130 (= L133), L164 (= L173)

7nlnA Crystal structure of mycobacterium tuberculosis argb in complex with n-acetyl-glutamate
47% identity, 95% coverage: 9:293/301 of query aligns to 5:285/290 of 7nlnA

query
sites
7nlnA

S

T

H

H

V

I

-

K

A

A

E

Q

V

V

L

L

S

A

E

E

A

A

L

L

P

P

Y

W

I

L

R

K

R

Q

F

L

V

H

G

G

K

K

T

V

L

V

V

V

I

V

K

K

Y

Y

G

G

N

N

A

A

M

M

E

T

S

D

E

D

E

T

L

L

K

R

T

R

G

A

F

F

A

A

R

A

D

D

I

M

V

A

L

F

M

L

K

R

A

N

V

C

G

G

I

I

N

H

P

P

V

V

H

H

G
|
G

P

P

Q

Q

I
|
I

G

T

D

A

L

M

L

L

K

R

R

R

L

L

S

G

I

I

E

E

S

G

H

D

F

F

I

K

D

G

G

G

M
x
F

R
|
R

V

V

T

T

D

T

S

P

A

E

T

V

M

L

D

D

V

V

V

A

E

R

M

M

V

V

L
|
L

G

F

G

G

Q

Q

V

V

N

G

K

R

D

E

I

L

V

V

N

N

L

L

I

I

N

N

R

A

H

H

G

G

G

P

S

Y

A

A

I

V

G

G

L

I

T

T

G

G

K

E

D

D

A

A

E

Q

L

L

I

F

R

T

A

A

R

V

K

R

L

R

T

S

V

V

S

T

R

V

Q

D

T

G

P

V

E

A

M

T

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

L

V
|
V

G

G

E

D

V

V

V

D

S

Q

V

V

N

N

T

T

D

A

L

A

L

M

N

L

M

D

L

L

V

V

K

A

G

A

D

G

F

R

I

I

P

P

V

V

I

V

A

S

P

T

I

L

G

A

V

P

G

D

A

A

N

D

G

G

E

V

S

V

Y

H

N
|
N

I
|
I

N
|
N

A
|
A

D

D

L

T

V

A

A

A

G

A

K

A

V

V

A

A

E

E

A

A

L

L

K

G

A

A

E

E

K

K

L

L

M

L

L

M

L

L

T

T

N

D

I

I

A

D

G

G

L

L

M

Y

D

T

K

R

-

W

-

P

-

D

Q

R

G

D

Q

S

V

L

L

V

T

S

G

E

L

I

T

D

T

T

E

G

Q

T

V

L

N

A

E

Q

L

L

I

L

A

P

D

-

G

-

T

T

I

L

Y

E

G

S

G

G

M

M

L

V

P

P

K

K

I

V

K

E

C

A

A

C

L

L

D

R

A

A

V

V

Q

I

G

G

V

V

N

P

S

S

S

A

H

H

I

I

D

D

G

G

R

R

V

V

P

T

N

H

A

C

V

V

L

L

L

V

E

E

I

L

F

F

T

T

D

D

S

A

G

G

V

T

G

G

T

T

binding n-acetyl-l-glutamate: G64 (= G67), G65 (= G68), G66 (= G69), I69 (= I72), F86 (≠ M89), R87 (= R90), L101 (= L104), V150 (= V159), N186 (= N195), I187 (= I196), N188 (= N197), A189 (= A198)

7nlyA Crystal structure of mycobacterium tuberculosis argb in complex with 2-chlorobenzimidazole.
47% identity, 95% coverage: 9:293/301 of query aligns to 8:288/293 of 7nlyA

query
sites
7nlyA

S

T

H

H

V

I

-

K

A

A

E

Q

V

V

L

L

S

A

E

E

A

A

L

L

P

P

Y

W

I

L

R

K

R

Q

F

L

V

H

G

G

K

K

T

V

L

V

V

V

I

V

K

K

Y

Y

G

G

N

N

A

A

M

M

E

T

S

D

E

D

E

T

L

L

K

R

T

R

G

A

F

F

A

A

R

A

D

D

I

M

V

A

L

F

M

L

K

R

A

N

V

C

G

G

I

I

N

H

P

P

V

V

H

H

G

G

P

P

Q

Q

I

I

G

T

D

A

L

M

L

L

K

R

R

R

L

L

S

G

I

I

E

E

S

G

H

D

F

F

I

K

D

G

G

G

M

F

R

R

V

V

T

T

D

T

S

P

A

E

T

V

M

L

D

D

V

V

V

A

E

R

M

M

V

V

L

L

G

F

G

G

Q

Q

V

V

N

G

K

R

D

E

I

L

V

V

N

N

L

L

I

I

N

N

R

A

H

H

G

G

G

P

S

Y

A

A

I
x
V

G

G

L

I

T

T

G

G

K

E

D

D

A

A

E

Q

L

L

I

F

R

T

A

A

R

V

K

R

L

R

T

S

V

V

S

T

R

V

Q

D

T

G

P

V

E

A

M

T

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

L

V

V

G

G

E

D

V

V

V

D

S

Q

V

V

N

N

T

T

D

A

L

A

L

M

N

L

M

D

L
|
L

V

V

K

A

G

A

D

G

F

R

I

I

P

P

V

V

I

V

A

S

P

T

I

L

G

A

V

P

G

D

A

A

N

D

G

G

E

V

S

V

Y

H

N

N

I

I

N

N

A

A

D

D

L

T

V

A

A

A

G

A

K

A

V

V

A

A

E

E

A

A

L

L

K

G

A

A

E

E

K

K

L

L

M

L

L

M

L

L

T

T

N

D

I

I

A

D

G

G

L

L

M

Y

D

T

K

R

-

W

-

P

-

D

Q

R

G

D

Q

S

V

L

L

V

T

S

G

E

L

I

T

D

T

T

E

G

Q

T

V

L

N

A

E

Q

L

L

I

L

A

P

D

-

G

-

T

T

I

L

Y

E

G

S

G

G

M

M

L

V

P

P

K

K

I

V

K

E

C

A

A

C

L

L

D

R

A

A

V

V

Q

I

G

G

V

V

N

P

S

S

S

A

H

H

I

I

D

D

G

G

R

R

V

V

P

T

N

H

A

C

V

V

L

L

L

V

E

E

I

L

F

F

T

T

D

D

S

A

G

G

V

T

G

G

T

T

binding 2-chloranyl-1~{H}-benzimidazole: V124 (≠ I124), L167 (= L173)

7nloA Crystal structure of mycobacterium tuberculosis argb in complex with l-arginine
47% identity, 95% coverage: 9:293/301 of query aligns to 4:284/289 of 7nloA

query
sites
7nloA

S

T

H

H

V

I

-

K

A

A

E

Q

V

V

L

L

S

A

E

E

A

A

L

L

P

P

Y
x
W

I

L

R

K

R

Q

F

L

V

H

G

G

K

K

T

V

L

V

V

V

I

V

K

K

Y

Y

G

G

N

N

A

A

M

M

E

T

S

D

E

D

E

T

L

L

K

R

T

R

G

A

F

F

A

A

R

A

D

D

I

M

V

A

L

F

M

L

K

R

A

N

V

C

G

G

I

I

N

H

P

P

V

V

H

H

G

G

P

P

Q

Q

I

I

G

T

D

A

L

M

L

L

K

R

R

R

L

L

S

G

I

I

E

E

S

G

H

D

F

F

I

K

D

G

G

G

M

F

R

R

V

V

T

T

D

T

S

P

A

E

T

V

M

L

D

D

V

V

V

A

E

R

M

M

V

V

L

L

G

F

G

G

Q

Q

V

V

N

G

K

R

D

E

I

L

V

V

N

N

L

L

I

I

N

N

R

A

H

H

G

G

G

P

S

Y

A

A

I

V

G

G

L

I

T

T

G

G

K

E

D

D

A

A

E

Q

L

L

I

F

R

T

A

A

R

V

K

R

L

R

T

S

V

V

S

T

R

V

Q

D

T

G

P

V

E

A

M

T

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

L

V

V

G

G

E

D

V

V

V

D

S

Q

V

V

N

N

T

T

D

A

L

A

L

M

N

L

M

D

L

L

V

V

K

A

G

A

D

G

F

R

I

I

P

P

V

V

I

V

A

S

P

T

I

L

G

A

V

P

G

D

A

A

N

D

G

G

E

V

S

V

Y

H

N

N

I

I

N

N

A

A

D

D

L

T

V

A

A

A

G

A

K

A

V

V

A

A

E

E

A

A

L

L

K

G

A

A

E

E

K
|
K

L

L

M

L

L

M

L

L

T

T

N

D

I

I

A

D

G

G

L

L

M

Y

D

T

K

R

-

W

-

P

-

D

Q

R

G

D

Q

S

V

L

L

V

T
x
S

G

E

L

I

T

D

T

T

E

G

Q

T

V

L

N

A

E

Q

L

L

I

L

A

P

D

-

G

-

T

T

I

L

Y

E

G

S

G

G

M

M

L

V

P

P

K

K

I

V

K

E

C

A

A

C

L

L

D

R

A

A

V

V

Q

I

G

G

V

V

N

P

S

S

S

A

H
|
H

I

I

D

D

G

G

R

R

V

V

P

T

N

H

A

C

V

V

L

L

L

V

E
|
E

I
x
L

F

F

T
|
T

D
|
D

S
x
A

G
|
G

V

T

G
|
G

T
|
T

binding arginine: W17 (≠ Y21), K203 (= K213), S225 (≠ T232), H262 (= H271), E275 (= E284), L276 (≠ I285), T278 (= T287), D279 (= D288), A280 (≠ S289), G281 (= G290), G283 (= G292), T284 (= T293)

Sites not aligning to the query:

binding arginine: 285

7nlpA Crystal structure of mycobacterium tuberculosis argb in complex with l-canavanine
47% identity, 95% coverage: 9:293/301 of query aligns to 7:287/292 of 7nlpA

query
sites
7nlpA

S

T

H

H

V

I

-

K

A

A

E

Q

V

V

L

L

S

A

E

E

A

A

L

L

P

P

Y
x
W

I

L

R

K

R

Q

F

L

V

H

G

G

K

K

T

V

L

V

V

V

I

V

K

K

Y

Y

G

G

N

N

A

A

M

M

E

T

S

D

E

D

E

T

L

L

K

R

T

R

G

A

F

F

A

A

R

A

D

D

I

M

V

A

L

F

M

L

K

R

A

N

V

C

G

G

I

I

N

H

P

P

V

V

H

H

G

G

P

P

Q

Q

I

I

G

T

D

A

L

M

L

L

K

R

R

R

L

L

S

G

I

I

E

E

S

G

H

D

F

F

I

K

D

G

G

G

M

F

R

R

V

V

T

T

D

T

S

P

A

E

T

V

M

L

D

D

V

V

V

A

E

R

M

M

V

V

L

L

G

F

G

G

Q

Q

V

V

N

G

K

R

D

E

I

L

V

V

N

N

L

L

I

I

N

N

R

A

H

H

G

G

G

P

S

Y

A

A

I

V

G

G

L

I

T

T

G

G

K

E

D

D

A

A

E

Q

L

L

I

F

R

T

A

A

R

V

K

R

L

R

T

S

V

V

S

T

R

V

Q

D

T

G

P

V

E

A

M

T

T

-

P

-

E

-

I

-

D

D

I

I

G

G

H

L

V

V

G

G

E

D

V

V

V

D

S

Q

V

V

N

N

T

T

D

A

L

A

L

M

N

L

M

D

L

L

V

V

K

A

G

A

D

G

F

R

I

I

P

P

V

V

I

V

A

S

P

T

I

L

G

A

V

P

G

D

A

A

N

D

G

G

E

V

S

V

Y

H

N

N

I

I

N

N

A

A

D

D

L

T

V

A

A

A

G

A

K

A

V

V

A

A

E

E

A

A

L

L

K

G

A

A

E

E

K
|
K

L

L

M

L

L

M

L

L

T

T

N

D

I

I

A

D

G

G

L

L

M

Y

D

T

K

R

-

W

-

P

-

D

Q

R

G

D

Q

S

V

L

L

V

T
x
S

G

E

L

I

T

D

T

T

E

G

Q

T

V

L

N

A

E

Q

L

L

I

L

A

P

D

-

G

-

T

T

I

L

Y

E

G

S

G

G

M

M

L

V

P

P

K

K

I

V

K

E

C

A

A

C

L

L

D

R

A

A

V

V

Q

I

G

G

V

V

N

P

S

S

S

A

H
|
H

I

I

D

D

G

G

R

R

V

V

P

T

N

H

A

C

V

V

L

L

L

V

E
|
E

I
x
L

F

F

T
|
T

D
|
D

S
x
A

G
|
G

V

T

G
|
G

T
|
T

binding l-canavanine: