SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing PP_5341 FitnessBrowser__Putida:PP_5341 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 18 hits to proteins with known functional sites (download)

Q9I6J1 Putrescine-binding periplasmic protein SpuD from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
60% identity, 100% coverage: 1:366/367 of query aligns to 2:366/367 of Q9I6J1

query
sites
Q9I6J1

M

M

V

K

R

R

L

F

K

G

R

K

L

T

L

L

A

L

P

A

F

L

I

T

A

L

A

A

T

G

L

S

F

V

A

A

G

G

A

M

L

A

H

Q

A

A

Q

-

A

A

E

D

Q

N

R

K

T

V

L

L

R

H

V

V

Y

Y

N

N

W

W

F
x
S

D
|
D

Y

Y

I

I

T

A

P

P

Q

D

T

T

L

L

T

E

A

K

F

F

Q

T

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

Y

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

A

K

K

L

L

T

A

G

G

N

K

S

S

G

G

Y

Y

D

D

V

V

P

P

S

S

N

N

V

S

F

F

L

L

A

A

K

K

Q

Q

I

I

E

K

A

A

G

G

V

V

F

Y

Q

Q

P

K

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

N

W

W

Q

K

H

N

L

L

D

N

P

K

A

D

L

L

M

M

K

H

L

T

I

L

E

E

A

V

N

S

D

D

P

P

G

G

N

N

K

E

F

H

A

A

V

I

P

P

Y

Y

M

M

Y

W

G

G

T

T

V

I

L

G

I

I

G

G

F

Y

N

N

P

P

A

D

K

K

V

V

K

K

A

A

L

F

G

G

D

D

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

V

F

F

K

K

E

P

E

E

N

N

I

I

A

Q

K

K

L

L

K

K

Q

Q

C
|
C

G

G

V

V

A

S

L

F

L

L

D

D

S

S

P

P

S

T

E

E

I

I

L

L

P

P

L

A

A

A

L

L

Q

H

Y

Y

L

L

G

G

L

Y

P

K

P

P

N

D

S

T

D

D

K

N

P

P

A

K

D

E

Y

L

K

K

K

A

A

A

E

E

L

L

M

F

L

L

K

K

I

I

R

R

P

P

H

Y

I

V

T

T

Y

Y

F

F

H

H

S

S

K

K

Y

Y

M

I

A

S

D

D

I

L

A

A

N

N

G

G

D

N

I

I

C
|
C

V

V

A

A

V

I

G

G

Y

Y

S

S

G

G

S
x
D

F

I

S

Y

Q

Q

A

A

A

K

N

S

R

R

A

A

R

E

E

E

A

A

N

K

N

N

G

K

V

V

V

T

V

V

D

K

M

Y

R

N

L

I

P

P

K

K

E

E

G

G

A

A

P

G

I

S

W
x
F

F

F

D
|
D

M

M

L

V

A

A

I

I

P

P

K

K

G

D

A

A

A

E

N

N

P

T

E

E

D

G

A

A

H

L

T

A

F

F

I

V

N

N

Y

F

L

L

L

M

R

K

P

P

E

E

V

I

I

M

A

A

P

E

I

I

S

T

D

D

F

V

V

V

G

Q

Y

F

P

P

N

N

P

G

N

N

K

A

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

E

T

A

I

I

R

R

N

N

N

D

P

P

N

G

L

I

Y

Y

P

P

T

S

A

E

E

E

A

V

M

M

A

K

K

K

L

L

Y

Y

T

T

L

F

K

P

P

D

L

L

T

P

R

A

N

K

A

T

E

Q

R

R

A

A

R

M

T

T

R

R

A

S

W

W

T

T

R

K

I

I

K

K

S

S

G

G

SD 36:37 (≠ FD 36:37) binding
C173 (= C173) modified: Disulfide link with 236
C236 (= C236) modified: Disulfide link with 173
D244 (≠ S244) binding
F273 (≠ W273) mutation to W: Significant decrease in affinity for putrescine (to KD=0.75 uM).
D275 (= D275) binding

3ttmA Crystal structure of spud in complex with putrescine (see paper)
62% identity, 93% coverage: 26:366/367 of query aligns to 1:341/341 of 3ttmA

query
sites
3ttmA

E

D

Q

N

R

K

T

V

L

L

R

H

V

V

Y

Y

N

N

W
|
W

F
x
S

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

L

T

E

A

K

F

F

Q

T

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

Y

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

A

K

K

L

L

T

A

G

G

N

K

S

S

G

G

Y

Y

D

D

V

V

P

P

S

S

N

N

V

S

F

F

L

L

A

A

K

K

Q

Q

I

I

E

K

A

A

G

G

V

V

F

Y

Q

Q

P

K

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

N

W

W

Q

K

H

N

L

L

D

N

P

K

A

D

L

L

M

M

K

H

L

T

I

L

E

E

A

V

N

S

D

D

P

P

G

G

N

N

K

E

F

H

A

A

V

I

P

P

Y

Y

M

M

Y

W

G

G

T

T

V

I

L

G

I

I

G

G

F

Y

N

N

P

P

A

D

K

K

V

V

K

K

A

A

L

F

G

G

D

D

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

V

F

F

K

K

E

P

E

E

N

N

I

I

A

Q

K

K

L

L

K

K

Q

Q

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

S

P

P

S

T

E

E

I

I

L

L

P

P

L

A

A

A

L

L

Q

H

Y

Y

L

L

G

G

L

Y

P

K

P

P

N

D

S

T

D

D

K

N

P

P

A

K

D

E

Y

L

K

K

K

A

A

A

E

E

L

L

M

F

L

L

K

K

I

I

R

R

P

P

H

Y

I

V

T

T

Y

Y

F

F

H

H

S

S

K

K

Y

Y

M

I

A

S

D

D

I

L

A

A

N

N

G

G

D

N

I

I

C

C

V

V

A

A

V

I

G

G

Y
|
Y

S

S

G

G

S
x
D

F

I

S

Y

Q

Q

A

A

A

K

N

S

R

R

A

A

R

E

E

E

A

A

N

K

N

N

G

K

V

V

V

T

V

V

D

K

M

Y

R

N

L

I

P

P

K

K

E

E

G

G

A

A

P

G

I

S

W
x
F

F

F

D
|
D

M

M

L

V

A

A

I

I

P

P

K

K

G

D

A

A

A

E

N

N

P

T

E

E

D

G

A

A

H

L

T

A

F

F

I

V

N

N

Y

F

L

L

L

M

R

K

P

P

E

E

V

I

I

M

A

A

P

E

I

I

S

T

D

D

F

V

V

V

G

Q

Y
x
F

P

P

N

N

P

G

N

N

K

A

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

E

T

A

I

I

R

R

N

N

N

D

P

P

N

G

L

I

Y

Y

P

P

T

S

A

E

E

E

A

V

M

M

A

K

K

K

L

L

Y

Y

T

T

L

F

K

P

P

D

L

L

T

P

R

A

N

K

A

T

E

Q

R

R

A

A

R

M

T

T

R

R

A

S

W

W

T

T

R

K

I

I

K

K

S

S

G

G

binding 1,4-diaminobutane: W10 (= W35), S11 (≠ F36), D12 (= D37), Y13 (= Y38), Y216 (= Y241), D219 (≠ S244), F248 (≠ W273), D250 (= D275), F286 (≠ Y311)

P31133 Putrescine-binding periplasmic protein PotF from Escherichia coli (strain K12) (see 3 papers)
56% identity, 99% coverage: 5:366/367 of query aligns to 6:369/370 of P31133

query
sites
P31133

K
|
K

R
x
K

L
x
W

L
|
L

A
x
S

P
x
G

F
x
L

I
x
V

A
|
A

A
x
G

T
x
A

L
|
L

F
x
M

A
|
A

G
x
V

A
x
S

L
x
V

H
x
G

A
x
T

-
x
L

Q
x
A

A

A

E

E

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W

W

F
x
S

D

D

Y

Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C
|
C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C
|
C

V

V

A

A

V

I

G

G

Y

W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W

F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y

Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

A

A

E

D

A

V

M

R

A

A

K

K

L

L

Y

F

T

T

L

L

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

S38 (≠ F36) binding
C175 (= C173) modified: Disulfide link with 239
C239 (= C236) modified: Disulfide link with 175
D247 (≠ S244) binding
D278 (= D275) binding

Sites not aligning to the query:

1:26 signal peptide

7oyxB E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/347 of 7oyxB

query
sites
7oyxB

Q

Q

R
x
K

T
|
T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I
x
V

F

F

D

D

T

S

N
|
N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L
x
M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

S

F

Y

L

L

A
x
E

K
x
R

Q

Q

I

L

E
x
T

A
|
A

G
|
G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L

L

M

L

K

K

L
|
L

I

V

E

A

A
x
K

N
x
H

D

D

P
|
P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A
x
K

N
|
N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N
x
S

P
x
A

T
x
E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

A

A

E

D

A

V

M
x
R

A

A

K

K

L

L

Y

F

T

T

L

Q

K

K

P
x
V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ R28), T3 (= T29), T10 (≠ F36), D11 (= D37), V33 (≠ I59), L91 (= L117), W107 (≠ Y133), K259 (≠ A284), N260 (= N285), R314 (≠ M339)
binding (2~{R})-1-[(2~{R})-1-[(2~{S})-1-[(2~{S})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-yl]oxypropan-2-amine: E62 (≠ A88), R63 (≠ K89), T66 (≠ E92), K94 (≠ A120), H95 (≠ N121), P97 (= P123), V322 (≠ P347)
binding (2~{R})-1-(2-methoxyethoxy)propan-2-amine: S298 (≠ N323), A299 (≠ P324), E300 (≠ T325)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: M45 (≠ L71), A67 (= A93), G68 (= G94)
binding spermidine: W9 (= W35), T10 (≠ F36), D11 (= D37), Y12 (= Y38), N37 (= N63), S57 (= S83), E157 (= E183), W216 (≠ Y241), D219 (≠ S244), W248 (= W273), D250 (= D275), Y286 (= Y311)

7oyxA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d y87s f88y s247d in complex with spermidine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/347 of 7oyxA

query
sites
7oyxA

Q

Q

R
x
K

T
|
T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N
|
N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L
x
M

T

A

G
|
G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

S

F

Y

L

L

A

E

K

R

Q

Q

I

L

E

T

A
|
A

G
|
G

V
|
V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L
|
L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A
x
K

N
|
N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N
|
N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y
|
Y

P

P

T

P

A

A

E

D

A

V

M
x
R

A

A

K

K

L

L

Y

F

T

T

L

Q

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: K2 (≠ R28), T3 (= T29), M45 (≠ L71), G47 (= G73), V69 (= V95), K259 (≠ A284), N260 (= N285)
binding (2~{S})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L88 (= L114), L91 (= L117), W107 (≠ Y133), N288 (= N313), Y308 (= Y333), R314 (≠ M339)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: A67 (= A93), G68 (= G94)
binding spermidine: W9 (= W35), T10 (≠ F36), D11 (= D37), Y12 (= Y38), N37 (= N63), S57 (= S83), E157 (= E183), W216 (≠ Y241), D219 (≠ S244), W248 (= W273), D250 (= D275), Y286 (= Y311)

6ye7A E.Coli's putrescine receptor potf complexed with cadaverine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/341 of 6ye7A

query
sites
6ye7A

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

A

A

E

D

A

V

M

R

A

A

K

K

L

L

Y

F

T

T

L

L

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding pentane-1,5-diamine: W9 (= W35), S10 (≠ F36), D11 (= D37), Y12 (= Y38), W216 (≠ Y241), D219 (≠ S244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

6ye6A E.Coli's putrescine receptor potf complexed with agmatine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/341 of 6ye6A

query
sites
6ye6A

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D
|
D

Y
|
Y

I
|
I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q
x
E

K
|
K

D
x
E

S
x
T

G
|
G

V

I

K
|
K

L

V

V

V

Y
|
Y

D

D

I
x
V

F
|
F

D
|
D

T

S

N
|
N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D
|
D

P

P

A

E

L

L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D
x
T

K
|
K

P
x
A

A
x
D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

S
|
S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S
x
W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M
x
F

R
x
S

L
x
I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T
x
Q

F

F

I

L

N
|
N

Y
|
Y

L

L

R
|
R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N
|
N

P
x
A

N

N

K
|
K

D

A

A

A

T
|
T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N
x
G

L

I

Y
|
Y

P

P

T
x
P

A
|
A

E

D

A

V

M
x
R

A

A

K

K

L
|
L

Y

F

T

T

L

L

K

K

P

V

L

Q

T

D

R

P

N
x
K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding agmatine: W9 (= W35), S10 (≠ F36), D11 (= D37), Y12 (= Y38), N37 (= N63), S57 (= S83), E157 (= E183), W216 (≠ Y241), D219 (≠ S244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)
binding (2R)-1-methoxypropan-2-amine: S10 (≠ F36), S10 (≠ F36), D11 (= D37), I13 (= I39), E24 (≠ D50), T25 (≠ S51), Y31 (= Y57), V33 (≠ I59), V33 (≠ I59), F34 (= F60), D35 (= D61), D85 (= D111), W107 (≠ Y133), T173 (≠ D199), K174 (= K200), A175 (≠ P201), D176 (≠ A202), S199 (= S224), W221 (≠ S246), F238 (≠ M263), S239 (≠ R264), I240 (≠ L265), Q266 (≠ T291), N269 (= N294), Y270 (= Y295), R273 (= R298), N288 (= N313), A289 (≠ P314), K291 (= K316), T294 (= T319), G306 (≠ N331), Y308 (= Y333), P310 (≠ T335), A311 (= A336), R314 (≠ M339), K326 (≠ N351)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: E22 (≠ Q48), K23 (= K49), G26 (= G52), L91 (= L117), W107 (≠ Y133), R314 (≠ M339), L317 (= L342)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: E22 (≠ Q48), K28 (= K54)

6ye0A E.Coli's putrescine receptor potf complexed with putrescine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/341 of 6ye0A

query
sites
6ye0A

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I
x
V

F
|
F

D
|
D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L
x
M

T

A

G
|
G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A
|
A

G
|
G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L
|
L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

S
|
S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F
|
F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

A

A

E

D

A

V

M
x
R

A

A

K

K

L

L

Y

F

T

T

L

L

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding (2R)-1-methoxypropan-2-amine: S10 (≠ F36), V33 (≠ I59), F34 (= F60), D35 (= D61), S199 (= S224)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: M45 (≠ L71), G47 (= G73), A67 (= A93), G68 (= G94), L88 (= L114), L91 (= L117), W107 (≠ Y133), F249 (= F274), R314 (≠ M339)
binding 1,4-diaminobutane: W9 (= W35), S10 (≠ F36), D11 (= D37), Y12 (= Y38), W216 (≠ Y241), D219 (≠ S244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

1a99A Putrescine receptor (potf) from e. Coli (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/341 of 1a99A

query
sites
1a99A

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
S

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S

S

N

A

V

S

F

F

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L

L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y

W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

A

P

P

S

E

E

E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
x
F

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T

P

A

A

E

D

A

V

M

R

A

A

K

K

L

L

Y

F

T

T

L

L

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding 1,4-diaminobutane: W9 (= W35), S10 (≠ F36), D11 (= D37), Y12 (= Y38), W216 (≠ Y241), D219 (≠ S244), F248 (≠ W273), D250 (= D275), Y286 (= Y311)

7oywA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88l s247d in complex with spermidine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/348 of 7oywA

query
sites
7oywA

Q

Q

R

K

T

T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q

E

K

K

D

E

S

T

G

G

V

I

K

K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N
|
N

E

E

A

V

L

L

E

E

A

G

K

K

L
|
L

L
x
M

T

A

G
|
G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V

Y

F

L

L

L

A

E

K

R

Q

Q

I

L

E

T

A
|
A

G
|
G

V
|
V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L
|
L

M

L

K

K

L

L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A

K

N

N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P
x
A

N
|
N

P

A

N
|
N

K
|
K

D

A

A

A

T
|
T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y
|
Y

P

P

T
x
P

A
|
A

E
x
D

A

V

M
x
R

A

A

K

K

L

L

Y

F

T

T

L

Q

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: L44 (= L70), M45 (≠ L71), G47 (= G73), A67 (= A93), G68 (= G94), V69 (= V95)
binding (2~{S})-1-(2-methoxyethoxy)propan-2-amine: P310 (≠ T335), A311 (= A336), D312 (≠ E337), R314 (≠ M339)
binding (2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-amine: L88 (= L114), W107 (≠ Y133), A287 (≠ P312), N288 (= N313), N290 (= N315), K291 (= K316), T294 (= T319), Y308 (= Y333)
binding spermidine: W9 (= W35), T10 (≠ F36), D11 (= D37), Y12 (= Y38), N37 (= N63), S57 (= S83), E157 (= E183), W216 (≠ Y241), D219 (≠ S244), W248 (= W273), D250 (= D275), Y286 (= Y311)

7oyvA E.Coli's putrescine receptor variant potf/d (4jdf) with mutations e39d f88a s247d in complex with spermidine (see paper)
57% identity, 93% coverage: 27:366/367 of query aligns to 1:341/341 of 7oyvA

query
sites
7oyvA

Q
|
Q

R
x
K

T
|
T

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D
|
D

Y
|
Y

I

I

T

A

P

P

Q

D

T

T

L

V

T

A

A

N

F

F

Q
x
E

K
|
K

D

E

S

T

G
|
G

V
x
I

K
|
K

L

V

V

V

Y

Y

D

D

I

V

F

F

D

D

T

S

N
|
N

E

E

A

V

L

L

E

E

A

G

K

K

L

L

L

M

T

A

G

G

N

S

S

T

G

G

Y

F

D

D

V

L

V

V

P

P

S
|
S

N

A

V
x
Y

F

A

L

L

A

E

K

R

Q

Q

I

L

E

T

A

A

G

G

V

V

F

F

Q

Q

P

P

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

E

W

W

Q

K

H

N

L

L

D

D

P

P

A

E

L

L

M

L

K

K

L
|
L

I

V

E

A

A

K

N

H

D

D

P

P

G

D

N

N

K

K

F

F

A

A

V

M

P

P

Y

Y

M

M

Y
x
W

G

A

T

T

V

T

L

G

I

I

G

G

F

Y

N

N

P

V

A

D

K

K

V

V

K

K

A

A

A

V

L

L

G

G

D

E

N

N

A

A

P

P

V

V

D

D

S

S

W

W

D

D

L

L

I

I

F

L

K

K

E

P

E

E

N

N

I

L

A

E

K

K

L

L

K

K

Q

S

C

C

G

G

V

V

A

S

L

F

L

L

D

D

S

D

P

P

S

E

E
|
E

I

V

L

F

P

A

L

T

A

V

L

L

Q

N

Y

Y

L

L

G

G

L

K

P

D

P

P

N

N

S

S

D

T

K

K

P

A

A

D

D

D

Y

Y

K

T

K

G

-

P

A

A

E

T

E

D

L

L

M

L

L

L

K

K

I

L

R

R

P

P

H

N

I

I

T

R

Y

Y

F

F

H

H

S

S

K

Q

Y

Y

M

I

A

N

D

D

I

L

A

A

N

N

G

G

D

D

I

I

C

C

V

V

A

A

V

I

G

G

Y
x
W

S

A

G

G

S
x
D

F

V

S

W

Q

Q

A

A

A

S

N

N

R

R

A

A

R

K

E

E

A

A

N

K

N

N

G

G

V

V

V

N

V

V

D

S

M

F

R

S

L

I

P

P

K

K

E

E

G

G

A

A

P

M

I

A

W
|
W

F

F

D
|
D

M

V

L

F

A

A

I

M

P

P

K

A

G

D

A

A

A
x
K

N
|
N

P

K

E

D

D

E

A

A

H

Y

T

Q

F

F

I

L

N

N

Y

Y

L

L

R

R

P

P

E

D

V

V

I

V

A

A

P

H

I

I

S

S

D

D

F

H

V

V

G

F

Y
|
Y

P

A

N

N

P

A

N

N

K

K

D

A

A

A

T

T

D

P

K

L

V

V

N

S

P

A

T

E

I

V

R

R

N

E

N

N

P

P

N

G

L

I

Y

Y

P

P

T
x
P

A
|
A

E

D

A

V

M
x
R

A

A

K

K

L

L

Y

F

T

T

L

Q

K

K

P

V

L

Q

T

D

R

P

N

K

A

I

E

D

R

R

A

V

R

R

T

T

R

R

A

A

W

W

T

T

R

K

I

V

K

K

S

S

G

G

binding (2~{S})-1-methoxypropan-2-amine: Q1 (= Q27), K2 (≠ R28), T3 (= T29), L91 (= L117), W107 (≠ Y133), K259 (≠ A284), N260 (= N285), R314 (≠ M339)
binding (2~{R})-1-[(2~{R})-1-[(2~{R})-1-(2-methoxyethoxy)propan-2-yl]oxypropan-2-yl]oxypropan-2-amine: Q1 (= Q27), E22 (≠ Q48), K23 (= K49), G26 (= G52), I27 (≠ V53), K28 (= K54)
binding (2R)-1-methoxypropan-2-amine: Q1 (= Q27), K2 (≠ R28), P310 (≠ T335), A311 (= A336), R314 (≠ M339)
binding spermidine: W9 (= W35), T10 (≠ F36), D11 (= D37), Y12 (= Y38), N37 (= N63), S57 (= S83), Y59 (≠ V85), E157 (= E183), W216 (≠ Y241), D219 (≠ S244), W248 (= W273), D250 (= D275), Y286 (= Y311)

Q9I6J0 Spermidine-binding periplasmic protein SpuE from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
55% identity, 97% coverage: 11:366/367 of query aligns to 10:364/365 of Q9I6J0

query
sites
Q9I6J0

F

L

I

V

A

A

T

L

L

A

F

T

A

A

G

I

A

A

L

G

H

P

A

V

Q

Q

A

A

E

E

Q

K

R

K

T

S

L

L

R

H

V

I

Y

Y

N

N

W

W

F
x
T

D

D

Y

Y

I

I

T

A

P

P

Q

T

T

T

L

L

T

K

A

D

F

F

Q

T

K

K

D

E

S

S

G

G

V

I

K

D

L

V

V

S

Y

Y

D

D

I

V

F

F

D

D

T

S

N

N

E

E

A

T

L

L

E

E

A

G

K

K

L

L

L

V

T

S

G

G

N

H

S

S

G

G

Y

Y

D

D

V

I

V

V

P

P

S

S

N

N

V

N

F

F

L

L

A

G

K

K

Q

Q

I

I

E

Q

A

A

G

G

V

A

F

F

Q

Q

P

K

L

L

D

D

R

K

S

S

K

K

L

L

P

P

N

N

W

W

Q

K

H

N

L

L

D

D

P

P

A

A

L

L

M

L

K

K

L

Q

I

L

E

E

A

V

N

S

D

D

P

P

G

G

N

N

K

Q

F

Y

A

A

V

V

P

P

Y

Y

M

L

Y

W

G

G

T

T

V

N

L

G

I

I

G

G

F

Y

N

N

P

V

A

A

K

K

V

V

K

K

A

E

A

V

L

L

G

G

D

D

N

Q

A

-

P

P

V

I

D

D

S

S

W

W

D

A

L

I

I

L

F

F

K

E

E

P

E

E

N

N

I

M

A

K

K

K

L

L

K

A

Q

K

C

C

G

G

V

V

A

A

L

F

L

M

D

D

S

S

P

G

S

D

E
|
E

I

M

L

L

P

P

L

A

A

A

L

L

Q

N

Y

Y

L

L

G

G

L

L

P

D

P

P

N

N

S

T

D

H

K

D

P

P

A

K

D

D

Y

Y

K

K

A

A

E

E

L

V

M

L

L

T

K

K

I

V

R

R

P

P

H

Y

I

V

T

S

Y

Y

F

F

H

H

S

S

K

K

Y

Y

M

I

A

S

D

D

I

L

A

A

N

N

G

G

D

N

I

I

C

C

V

V

A

A

V

F

G

G

Y

Y

S

S

G

G

S
x
D

F

V

S

F

Q

Q

A

A

N

A

R

R

A

A

R

E

E

E

A

A

N

G

N

K

G

G

V

I

V

D

V

I

D

Q

M

Y

R

V

L

I

P

P

K

K

E

E

G

G

A

A

P
x
N

I

L

W
|
W

F

F

D

D

M

L

L

M

A

A

I

I

P

P

K

A

G

D

A

A

A

K

N

A

P

A

E

D

D

N

A

A

H

Y

T

A

F

F

I

I

N

D

Y

Y

L

L

R

R

P

P

E

E

V

V

I

I

A

A

P

K

I

V

S

S

D

D

F

Y

V

V

G

G

Y

Y

P

A

N

N

P

A

N

I

K

P

D

G

A

A

T

R

D

P

K

L

V

M

N

D

P

K

T

S

I

V

R

S

N

D

N

S

P

E

N

E

L

V

Y

Y

P

P

T

P

A

Q

E

A

A

V

M

L

A

D

K

K

L

L

Y

Y

T

V

L

S

K

A

P

V

L

L

T

P

R

A

N

K

A

V

E

L

R

R

A

L

R

Q

T

T

R

R

A

T

W

W

T

T

R

R

I

I

K

K

S

T

G

G

T35 (≠ F36) binding
E181 (= E183) binding
D242 (≠ S244) binding
N269 (≠ P271) binding
W271 (= W273) mutation to F: Increases affinity for putrescine (to KD=1.12 uM).

3ttnA Crystal structures of polyamine receptors spud and spue from pseudomonas aeruginosa (see paper)
56% identity, 92% coverage: 29:364/367 of query aligns to 1:335/335 of 3ttnA

query
sites
3ttnA

T

S

L

L

R

H

V

I

Y

Y

N

N

W
|
W

F
x
T

D

D

Y
|
Y

I

I

T

A

P

P

Q

T

T

T

L

L

T

K

A

D

F

F

Q

T

K

K

D

E

S

S

G

G

V

I

K

D

L

V

V

S

Y

Y

D

D

I

V

F

F

D

D

T

S