SitesBLAST

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
38% identity, 62% coverage: 2:241/385 of query aligns to 3:234/371 of 3puyA

query
sites
3puyA

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F
x
W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K

V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

D

R
|
R

Y

K

P

P

R

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D

D

E
|
E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H
|
H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F11), S37 (= S40), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), Q81 (= Q86), R128 (= R135), A132 (≠ E139), S134 (= S141), G136 (= G143), Q137 (= Q144), E158 (= E165), H191 (= H198)
binding magnesium ion: S42 (= S45), Q81 (= Q86)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
38% identity, 62% coverage: 2:241/385 of query aligns to 3:234/371 of 3puxA

query
sites
3puxA

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F
x
W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K

V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

D

R
|
R

Y

K

P

P

R

K

E

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D

D

E

E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H
|
H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F11), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), R128 (= R135), S134 (= S141), Q137 (= Q144)
binding beryllium trifluoride ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q86), S134 (= S141), G136 (= G143), H191 (= H198)
binding magnesium ion: S42 (= S45), Q81 (= Q86)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
38% identity, 62% coverage: 2:241/385 of query aligns to 3:234/371 of 3puwA

query
sites
3puwA

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F
x
W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K
x
V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

D

R
|
R

Y

K

P

P

R

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D

D

E
|
E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H
|
H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F11), V17 (≠ K19), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), R128 (= R135), A132 (≠ E139), S134 (= S141), Q137 (= Q144)
binding tetrafluoroaluminate ion: S37 (= S40), G38 (= G41), K41 (= K44), Q81 (= Q86), S134 (= S141), G135 (= G142), G136 (= G143), E158 (= E165), H191 (= H198)
binding magnesium ion: S42 (= S45), Q81 (= Q86)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
38% identity, 62% coverage: 2:241/385 of query aligns to 3:234/371 of 3puvA

query
sites
3puvA

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F
x
W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K
x
V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

D

R
|
R

Y

K

P

P

R

K

E
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D

D

E

E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H

H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F11), V17 (≠ K19), G38 (= G41), C39 (= C42), G40 (= G43), K41 (= K44), S42 (= S45), T43 (= T46), R128 (= R135), A132 (≠ E139), S134 (= S141), Q137 (= Q144)
binding magnesium ion: S42 (= S45), Q81 (= Q86)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
38% identity, 62% coverage: 2:241/385 of query aligns to 4:235/371 of P68187

query
sites
P68187

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F

W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K

V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G
x
A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D
x
K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A
x
V

R

N

E

Q

L
x
V

M

A

S
x
E

M

V

I

L

K

Q

L

L

E

-

P

-

K
x
A

Q

H

Y

L

L

L

N

D

R

R

Y

K

P

P

R

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D
|
D

E

E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H

H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D
x
R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

A85 (≠ G89) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ D110) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A118) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L121) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ S123) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ K130) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G143) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D164) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ D234) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
38% identity, 62% coverage: 2:241/385 of query aligns to 1:232/367 of 1q12A

query
sites
1q12A

I

V

E

Q

L

L

Q

Q

N

N

L

V

S

T

K

K

T

A

F
x
W

Q

-

S

-

N

-

G

G

K

E

D

V

V

V

K

V

A

S

V

K

D

D

S

-

V

I

S

N

L

L

T

D

V

I

N

H

E

E

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

-

G

G

E

E

D

K

T

R

T

M

D

N

L

-

D

D

E

T

V

P

T

P

L

A

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

I

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

D

R
|
R

Y

K

P

P

R

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

S

L

V

L

F

L

L

M

L

D

D

E

E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H

H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: W10 (≠ F11), S35 (= S40), G36 (= G41), C37 (= C42), G38 (= G43), K39 (= K44), S40 (= S45), T41 (= T46), R126 (= R135), A130 (≠ E139), S132 (= S141), G134 (= G143), Q135 (= Q144)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
40% identity, 62% coverage: 2:241/385 of query aligns to 7:230/353 of 1vciA

query
sites
1vciA

I

V

E

K

L

L

Q

E

N

N

L

L

S

T

K

K

T

R

F
|
F

Q

-

S

-

N

-

G

-

K

G

D

N

V
x
F

K

T

A

A

V

V

D

N

S

K

V

L

S

N

L

L

T

T

V

I

N

K

E

D

G

G

E

E

I

F

C

L

V

V

F

L

L

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

S
x
T

T
|
T

T

T

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

E

P

P

T

T

S

E

G

G

K

R

I

I

L

Y

I

F

N

G

G

D

E

R

D

D

T

V

T

T

D

Y

L

L

D

P

E

P

V

K

T

D

L

-

R

-

R

R

N

N

I

I

G

S

Y

M

V

V

I

F

Q

Q

Q

-

I

-

G

-

L

-

F

-

P

-

N

H

M

M

T

T

I

V

E

Y

E

E

N

N

I

I

T

A

I

F

V

-

P

-

R

P

L

L

L

K

G

K

W

F

D

P

K

K

Q

D

K

E

C

I

H

D

D

K

R

R

A

V

R

R

E

W

L

A

M

A

S

E

M

L

I

L

K

Q

L

I

E

E

P

-

K

-

Q

E

Y

L

L

L

N

N

R

R

Y

Y

P

P

R

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

I

V

G

A

V

V

I

A

R

R

A

A

L

I

A

V

D

E

A

P

P

D

L

V

L

L

M

M

D

D

E

E

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

K

N

L

R

R

E

V

M

A

I

M

Q

R

N

A

E

E

F

I

F

K

E

K

M

L

Q

Q

R

Q

A

K

L

L

N

K

K

V

T

T

V

T

I

I

M

Y

V

V

S

T

H

H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

M

G

G

D

D

K

R

I

I

A

A

I

V

F

M

R

N

A

R

G

G

K

Q

L

L

Q

Q

I

I

D

G

H

S

P

P

D

T

T

E

L

V

L

Y

A

L

H

R

P

P

A

N

D

S

D

V

F

F

V

V

S

A

N

T

F

F

V

I

G

G

binding adenosine-5'-triphosphate: F16 (= F11), F19 (≠ V18), S41 (= S40), G42 (= G41), G44 (= G43), K45 (= K44), T46 (≠ S45), T47 (= T46)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 62% coverage: 2:241/385 of query aligns to 4:235/369 of P19566

query
sites
P19566

I

V

E

Q

L

L

Q

R

N

N

L

V

S

T

K

K

T

A

F

W

Q

-

S

-

N

-

G

-

K

G

D

D

V

V

K

V

A

V

V

S

D

K

S

D

V

I

S

N

L

L

T

D

V

I

N

H

E

D

G

G

E

E

I

F

C

V

V

V

F

F

L

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

S

S

T

T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

K

T

P

I

T

T

S

S

G

G

K

D

I

L

L

F

I

I

N

G

G

E

E

T

D

R

T

M

T

N

D

D

L

I

D

P

E

P

V

A

T

-

L

-

R

E

R

R

N

G

I

V

G

G

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L
|
L

F

Y

P

P

N

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

T

S

I

F

V

G

P

L

R

K

L

L

L

A

G

G

W

A

D

K

K

K

Q

E

K

V

C

M

H

N

D

Q

R

R

A

V

R

N

E

Q

L

V

M

A

S

E

M

V

I

L

K

Q

L

L

E

-

P

-

K

A

Q

H

Y

L

L

L

N

E

R

R

Y

K

P

P

R

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

I

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

A

P

P

R

L

V

L

F

L

L

M

L

D

D

E

E

P
|
P

F

L

G

S

A

N

V

L

D
|
D

P

A

I

A

N

L

R

R

E

V

M

Q

I

M

Q

R

N

I

E

E

F

I

F

S

E

R

M

L

Q

H

R

K

A

R

L

L

N

G

K

R

T

T

V

M

I

I

M

Y

V

V

S

T

H

H

D

D

I

Q

D

V

E

E

A

A

I

M

K

T

L

L

G

A

D

D

K

K

I

I

A

V

I

V

F

L

R

D

A

A

G

G

K

R

L

V

L

A

Q

Q

I

V

D

G

H

K

P

P

D

L

T

E

L

L

L

Y

A

H

H

Y

P

P

A

A

D

D

D

R

F

F

V

V

S

A

N

G

F

F

V

I

G

G

L86 (= L90) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P166) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D171) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 63% coverage: 1:242/385 of query aligns to 17:250/378 of P69874

query
sites
P69874

M

L

I

V

E

Q

L

L

Q

A

N

G

L

I

S

R

K

K

T
x
C

F
|
F

Q

-

S

-

N

D

G

G

K

K

D

E

V

V

K

-

A

-

V

I

D

P

S

Q

V

L

S

D

L

L

T

T

V

I

N

N

E

N

G

G

E

E

I
x
F

C

L

V

T

F

L

L

L

G

G

P

P

S

S

G

G

C
|
C

G

G

K

K

S

T

T

T

T

V

L
|
L

K

R

M

L

I

I

N

A

R

G

L

L

I

E

K

T

P

V

T

D

S

S

G

G

K

R

I

I

L

M

I
x
L

N

D

G

N

E

E

D

D

T

I

T

T

D

H

L

V

D

P

E

-

V

-

T

A

L

E

R

N

R

R

N

Y

I

V

G

N

Y

T

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L

L

F

F

P

P

N

H

M

M

T

T

I

V

E

F

E

E

N

N

I

V

T

A

I

F

V

G

P

L

R

R

L

M

L

Q

G

K

W

T

D

P

K

A

Q

A

K

E

C

I

H

T

D

P

R

R

A

V

R

M

E

E

L

A

M

L

S

R

M

M

I
x
V

K

Q

L

L

E

E

P

T

K

-

Q

-

Y

F

L

A

N

Q

R

R

Y

K

P

P

R

H

E

Q

L

L

S

S

G

G

Q

Q

R

R

I

V

G

A

V

I

I

A

R

R

A

A

L

V

A

V

A

N

D

K

A

P

P

R

L

L

M

L

D
|
D

E

E

P

S

F

L

G

S

A

A

V

L

D

D

P

Y

I

K

N

L

R

R

E

K

M

Q

I

M

Q

Q

N

N

E

E

F

L

F

K

E

A

M

L

Q

Q

R

R

A

K

L

L

N

G

K

I

T

T

V

F

I

V

M

F

V

V

S

T

H

H

D

D

I

Q

D

E

E

E

A

A

I

L

K

T

L

M

G

S

D

D

K

R

I

I

A

V

I

V

F

M

R

R

A

D

G

G

K

R

L

I

L

E

Q

Q

I

D

D

G

H

T

P

P

D

R

T

E

L

I

L

Y

A

E

H

E

P

P

A

K

D

N

D

L

F

F

V

V

S

A

N

G

F

F

V

I

G

G

Q

E

C26 (≠ T10) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F11) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ I33) mutation to L: Lower ATPase activity and transport efficiency.
C54 (= C42) mutation to T: Loss of ATPase activity and transport.
L60 (= L48) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I64) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ I125) mutation to M: Loss of ATPase activity and transport.
D172 (= D164) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
36% identity, 63% coverage: 1:242/385 of query aligns to 2:235/358 of 8y5iA

query
sites
8y5iA

M

L

I

V

E

Q

L

L

Q

A

N

G

L

I

S

R

K

K

T

C

F
|
F

Q

-

S

-

N

D

G

G

K
|
K

D

E

V

V

K

-

A

-

V

I

D

P

S

Q

V

L

S

D

L

L

T

T

V

I

N

N

E

N

G

G

E

E

I

F

C

L

V

T

F

L

L

L

G

G

P

P

S

S

G
|
G

C

C

G

G

K
|
K

S
x
T

T
|
T

T

V

L

L

K

R

M

L

I

I

N

A

R

G

L

L

I

E

K

T

P

V

T

D

S

S

G

G

K

R

I

I

L

M

I

L

N

D

G

N

E

E

D

D

T

I

T

T

D

H

L

V

D

P

E

-

V

-

T

A

L

E

R

N

R

R

N

Y

I

V

G

N

Y

T

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L

L

F

F

P

P

N

H

M

M

T

T

I

V

E

F

E

E

N

N

I

V

T

A

I

F

V

G

P

L

R

R

L

M

L

Q

G

K

W

T

D

P

K

A

Q

A

K

E

C

I

H

T

D

P

R

R

A

V

R

M

E

E

L

A

M

L

S

R

M

M

I

V

K

Q

L

L

E

E

P

T

K

-

Q

-

Y

F

L

A

N

Q

R
|
R

Y

K

P

P

R

H

E
x
Q

L

L

S
|
S

G

G

Q

Q

R

R

I

V

G

A

V

I

I

A

R

R

A

A

L

V

A

V

A

N

D

K

A

P

P

R

L

L

M

L

D

D

E

Q

P

S

F

L

G

S

A

A

V

L

D

D

P

Y

I

K

N

L

R

R

E

K

M

Q

I

M

Q

Q

N

N

E

E

F

L

F

K

E

A

M

L

Q

Q

R

R

A

K

L

L

N

G

K

I

T

T

V

F

I

V

M

F

V

V

S

T

H

H

D

D

I

Q

D

E

E

E

A

A

I

L

K

T

L

M

G

S

D

D

K

R

I

I

A

V

I

V

F

M

R

R

A

D

G

G

K

R

L

I

L

E

Q

Q

I

D

D

G

H

T

P

P

D

R

T

E

L

I

L

Y

A

E

H

E

P

P

A

K

D

N

D

L

F

F

V

V

S

A

N

G

F

F

V

I

G

G

Q

E

binding adenosine-5'-triphosphate: F12 (= F11), K15 (= K16), G38 (= G41), K41 (= K44), T42 (≠ S45), T43 (= T46), R128 (= R135), Q132 (≠ E139), S134 (= S141)

3d31A Modbc from methanosarcina acetivorans (see paper)
35% identity, 64% coverage: 1:247/385 of query aligns to 1:235/348 of 3d31A

query
sites
3d31A

M

M

I

I

E

E

L

I

Q

E

N

S

L

L

S

S

K

R

T

K

F

W

Q

K

S

N

N

F

G

-

K

-

D

-

V

-

K

-

A

S

V

L

D

D

S

N

V

L

S

S

L

L

T

K

V

V

N

E

E

S

G

G

E

E

I

Y

C

F

V

V

F

I

L

L

G

G

P

P

S

T

G

G

C

A

G

G

K

K

S

T

T

L

T

F

L

L

K

E

M

L

I

I

N

A

R

G

L

F

I

H

K

V

P

P

T

D

S

S

G

G

K

R

I

I

L

L

I

L

N

D

G

G

E

K

D

D

T

V

T

T

D

D

L

L

D

S

E

P

V

E

T

-

L

-

R

K

R

H

N

D

I

I

G

A

Y

F

V

V

I

Y

Q

Q

Q

N

I

Y

G

S

L

L

F

F

P

P

N

H

M

M

T

N

I

V

E

K

N

N

I

L

T

E

I

-

V

-

P

-

R

-

L

-

L

F

G

G

W

M

D

R

K

M

Q

K

K

K

C

I

H

K

D

D

R

P

A

K

R

R

E

V

L

L

M

D

S

T

M

A

I

R

K

D

L

L

E

K

P

I

K

E

Q

H

Y

L

L

L

N

D

R

R

Y

N

P

P

R

L

E

T

L

L

S

S

G

G

Q

E

Q

Q

R

R

I

V

G

A

V

L

I

A

R

R

A

A

L

L

A

V

A

T

D

N

A

P

P

K

L

I

L

L

M

L

D

D

E

E

P

P

F

L

G

S

A

A

V

L

D

D

P

P

I

R

N

T

R

Q

E

E

M

N

I

A

Q

R

N

E

E

M

F

L

F

S

E

V

M

L

Q

H

R

K

A

K

L

N

N

K

K

L

T

T

V

V

I

L

M

H

V

I

S

T

H

H

D

D

I

Q

D

T

E

E

A

A

I

R

K

I

L

M

G

A

D

D

K

R

I

I

A

A

I

V

F

V

R

M

A

D

G

G

K

K

L

L

L

I

Q

Q

I

V

D

G

H

K

P

P

D

E

T

E

L

I

L

F

A

E

H

K

P

P

A

V

D

E

D

G

F

R

V

V

S

A

N

S

F

F

V

V

G

G

Q

F

D

E

S

N

T

V

L

L

K

K

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 62% coverage: 1:240/385 of query aligns to 1:241/343 of P30750

query
sites
P30750

M

M

I

I

E

K

L

L

Q

S

N

N

L

I

S

T

K

K

T

V

F

F

Q

H

S

Q

N

G

G

T

K

R

D

T

V

I

K

Q

A

A

V

L

D

N

S

N

V

V

S

S

L

L

T

H

V

V

N

P

E

A

G

G

E

Q

I

I

C

Y

V

G

F

V

L

I

G

G

P

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

T

L

L

I

K

R

M

C

I

V

N

N

R

L

L

L

I

E

K

R

P

P

T

T

S

E

G

G

K

S

I

V

L

L

I

V

N

D

G

G

E

Q

D

E

T

L

T

T

D

T

L

L

D

S

E

E

V

S

T

E

L

L

-

T

-

K

-

A

R

R

N

Q

I

I

G

G

Y

M

V

I

I

F

Q

Q

Q

H

I

F

G

N

L

L

F

L

P

S

N

S

M

R

T

T

I

V

E

F

E

G

N

N

I

V

T

A

I

L

V

P

P

L

R

E

L

L

L

D

G

N

W

T

D

P

K

K

Q

D

K

E

C

V

H

K

D

R

R

R

A

V

R

T

E

E

L

L

M

L

S

S

M

L

I

V

K

G

L

L

E

G

P

D

K

K

Q

H

Y

-

L

-

N

D

R

S

Y

Y

P

P

R

S

E

N

L

L

S

S

G

G

Q

Q

Q

K

Q

Q

R

R

I

V

G

A

V

I

I

A

R

R

A

A

L

L

A

A

A

S

D

N

A

P

P

K

L

V

L

L

M

C

D

D

E
|
E

P

A

F

T

G

S

A

A

V

L

D

D

P

P

I

A

N

T

R

T

E

R

M

S

I

I

Q

L

N

E

E

L

F

L

F

K

E

D

M

I

Q

N

R

R

A

R

L

L

N

G

K

L

T

T

V

I

I

L

M

L

V

I

S

T

H

H

D

E

I

M

D

D

E

V

A

V

I

K

K

R

L

I

G

C

D

D

K

C

I

V

A

A

I

V

F

I

R

S

A

N

G

G

K

E

L

L

L

I

Q

E

I

Q

D

D

H

T

P

V

D

S

T

E

L

V

L

F

A

S

H

H

P

P

A

K

D

T

D

P

F

L

V

A

S

Q

N

K

F

F

V

I

40:46 (vs. 40:46, 86% identical) binding ATP
E166 (= E165) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

8hplC Lpqy-sugabc in state 1 (see paper)
37% identity, 58% coverage: 20:241/385 of query aligns to 16:233/384 of 8hplC

query
sites
8hplC

A

A

V

V

D

K

S

E

V

F

S

S

L

M

T

T

V

I

N

A

E

D

G

G

E

E

I

F

C

I

V

I

F

L

L

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

S
|
S

T
|
T

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

I

E

K

E

P

I

T

T

S

S

G

G

K

E

I

L

L

R

I

I

N

G

G

G

E

E

D

R

T

V

T

N

D

E

L

-

D

-

E

K

V

A

T

P

L

K

R

D

R

R

N

D

I

I

G

A

Y

M

V

V

I

F

Q

Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

T

A

I

F

V

P

P

L

R

T

L

L

L

A

G

K

W

V

D

P

K

K

Q

A

K

E

C

I

H

A

D

A

R

K

A

V

R

E

E

E

L

T

M

A

S

K

M

I

I

L

K

D

L

L

E

-

P

-

K

S

Q

E

Y

L

L

L

N

D

R

R

Y

K

P

P

R

G

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

I

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

S

A

P

P

K

L

A

L

F

L

L

M

M

D

D

E

Q

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

K

N

L

R

R

E

V

M

Q

I

M

Q

R

N

A

E

E

F

I

F

S

E

R

M

L

Q

Q

R

D

A

R

L

L

N

G

K

T

T

T

V

T

I

V

M

Y

V

V

S

T

H

H

D

D

I

Q

D

T

E

E

A

A

I

M

K

T

L

L

G

G

D

D

K

R

I

V

A

V

I

V

F

M

R

L

A

A

G

G

K

E

L

V

L

Q

Q

Q

I

I

D

G

H

T

P

P

D

D

T

E

L

L

L

Y

A

S

H

S

P

P

A

A

D

N

D

L

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: G37 (= G41), C38 (= C42), G39 (= G43), K40 (= K44), S41 (= S45), T42 (= T46)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

8hprC Lpqy-sugabc in state 4 (see paper)
37% identity, 62% coverage: 2:241/385 of query aligns to 3:235/363 of 8hprC

query
sites
8hprC

I

I

E

V

L

L

Q

D

N

R

L

V

S

T

K

K

T

S

F
x
Y

Q

-

S

-

N

-

G

-

K

P

D

D

V

V

K

R

A

A

-

A

V

V

D

K

S

E

V

F

S

S

L

M

T

T

V

I

N

A

E

D

G

G

E

E

I

F

C

I

V

I

F

L

L

V

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

S
|
S

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

I

E

K

E

P

I

T

T

S

S

G

G

K

E

I

L

L

R

I

I

N

G

G

G

E

E

D

R

T

V

T

N

D

E

L

-

D

-

E

K

V

A

T

P

L

K

R

D

R

R

N

D

I

I

G

A

Y

M

V

V

I

F

Q
|
Q

Q

S

I

Y

G

A

L

L

F

Y

P

P

N

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

T

A

I

F

V

P

P

L

R

T

L

L

L

A

G

K

W

V

D

P

K

K

Q

A

K

E

C

I

H

A

D

A

R

K

A

V

R

E

E

E

L

T

M

A

S

K

M

I

I

L

K

D

L

L

E

-

P

-

K

S

Q

E

Y

L

L

L

N

D

R

R

Y

K

P

P

R

G

E
x
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

I

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

S

A

P

P

K

L

A

L

F

L

L

M

M

D

D

E

Q

P

P

F

L

G

S

A

N

V

L

D

D

P

A

I

K

N

L

R

R

E

V

M

Q

I

M

Q

R

N

A

E

E

F

I

F

S

E

R

M

L

Q

Q

R

D

A

R

L

L

N

G

K

T

T

T

V

T

I

V

M

Y

V

V

S

T

H
|
H

D

D

I

Q

D

T

E

E

A

A

I

M

K

T

L

L

G

G

D

D

K

R

I

V

A

V

I

V

F

M

R

L

A

A

G

G

K

E

L

V

L

Q

Q

Q

I

I

D

G

H

T

P

P

D

D

T

E

L

L

L

Y

A

S

H

S

P

P

A

A

D

N

D

L

F

F

V

V

S

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: Y12 (≠ F11), S38 (= S40), G39 (= G41), G41 (= G43), K42 (= K44), S43 (= S45), Q82 (= Q86), Q133 (≠ E139), G136 (= G142), G137 (= G143), Q138 (= Q144), H192 (= H198)
binding magnesium ion: S43 (= S45), Q82 (= Q86)

Query Sequence

>Pf1N1B4_1134 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_1134
MIELQNLSKTFQSNGKDVKAVDSVSLTVNEGEICVFLGPSGCGKSTTLKMINRLIKPTSG
KILINGEDTTDLDEVTLRRNIGYVIQQIGLFPNMTIEENITIVPRLLGWDKQKCHDRARE
LMSMIKLEPKQYLNRYPRELSGGQQQRIGVIRALAADAPLLLMDEPFGAVDPINREMIQN
EFFEMQRALNKTVIMVSHDIDEAIKLGDKIAIFRAGKLLQIDHPDTLLAHPADDFVSNFV
GQDSTLKRLLLVKAEDAADNAPSVSPETPVAEALELMDELDRRYVVVTCAENKALGYVRR
RDLHRQTGTCAQYLREFNATAAYDEHLRILLSRMYEFNRSWLPVMDAERVFLGEVTQESI
AEYLSSGKSRGGKTSIVSPAEAAVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory