SitesBLAST

L86 (= L99) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (≠ T178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

3c4jA Abc protein artp in complex with atp-gamma-s
29% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 3c4jA

query
sites
3c4jA

L

I

Q

D

L

V

R

H

R

Q

I

L

S

K

K

K

R

S

Y
x
F

P

G

G

S

C

L

L

E

A
x
V

N

L

D

K

A

G

I

I

D

N

L

V

S

H

I

I

A

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E
x
P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

H

D

A

F

D

D

S

E

G

G

E

E

M

I

I

I

W

I

Q

D

G

G

Q

I

R

N

V

L

T

K

M

A

R

K

-

D

-

T

N

N

P

L

A

N

Q

K

A

V

R

R

G

E

L

-

G

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

Q

N

N

N

I

I

A

T

L

L

A

A

M

P

G

M

A

K

A

V

A

R

G

K

T

W

P

P

K

R

Q

E

-

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

A

K

L

D

E

K

P

A

E
x
H

R

A

L
x
Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

I

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E
|
E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

G

G

E

F

V

A

R

R

A

E

L

V

C

G

H

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

A

I

G

E

H

E

C

G

V

K

P

P

A

E

Q

D

C

L

S

F

D

D

R

R

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y23), V18 (≠ A28), P37 (≠ E47), S38 (≠ N48), G39 (= G49), S40 (≠ A50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54), H133 (≠ E141), Y135 (≠ L143), E164 (= E172)

3c41J Abc protein artp in complex with amp-pnp/mg2+
29% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 3c41J

query
sites
3c41J

L

I

Q

D

L

V

R

H

R

Q

I

L

S

K

K

K

R

S

Y
x
F

P

G

G

S

C

L

L

E

A
x
V

N

L

D

K

A

G

I

I

D

N

L

V

S

H

I

I

A

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E
x
P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K
x
R

I

C

I

L

Y

N

G

L

V

L

T

E

H

D

A

F

D

D

S

E

G

G

E

E

M

I

I

I

W

I

Q

D

G

G

Q

I

R

N

V

L

T

K

M

A

R

K

-

D

-

T

N

N

P

L

A

N

Q

K

A

V

R

R

G

E

L

-

G

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

Q

N

N

N

I

I

A

T

L

L

A

A

M

P

G

M

A

K

A

V

A

R

G

K

T

W

P

P

K

R

Q

E

-

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

A

K

L

D

E

K

P

A

E
x
H

R

A

L
x
Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

I

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D
|
D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

G

G

E

F

V

A

R

R

A

E

L

V

C

G

H

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

A

I

G

E

H

E

C

G

V

K

P

P

A

E

Q

D

C

L

S

F

D

D

R

R

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y23), V18 (≠ A28), P37 (≠ E47), S38 (≠ N48), G39 (= G49), S40 (≠ A50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54), R47 (≠ K57), H133 (≠ E141), Y135 (≠ L143)
binding magnesium ion: S43 (= S53), D163 (= D171)

2olkA Abc protein artp in complex with adp-beta-s
29% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 2olkA

query
sites
2olkA

L

I

Q

D

L

V

R

H

R

Q

I

L

S

K

K

K

R

S

Y
x
F

P

G

G

S

C
x
L

L

E

A
x
V

N

L

D

K

A

G

I

I

D

N

L

V

S

H

I

I

A

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

H

D

A

F

D

D

S

E

G

G

E

E

M

I

I

I

W

I

Q

D

G

G

Q

I

R

N

V

L

T

K

M

A

R

K

-

D

-

T

N

N

P

L

A

N

Q

K

A

V

R

R

G

E

L

-

G

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

Q

N

N

N

I

I

A

T

L

L

A

A

M

P

G

M

A

K

A

V

A

R

G

K

T

W

P

P

K

R

Q

E

-

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

A

K

L

D

E

K

P

A

E

H

R

A

L

Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

I

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

G

G

E

F

V

A

R

R

A

E

L

V

C

G

H

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

A

I

G

E

H

E

C

G

V

K

P

P

A

E

Q

D

C

L

S

F

D

D

R

R

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (≠ Y23), L16 (≠ C26), V18 (≠ A28), S38 (≠ N48), G39 (= G49), S40 (≠ A50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54)

2oljA Abc protein artp in complex with adp/mg2+
29% identity, 43% coverage: 14:236/521 of query aligns to 4:228/242 of 2oljA

query
sites
2oljA

L

I

Q

D

L

V

R

H

R

Q

I

L

S

K

K

K

R

S

Y
x
F

P

G

G

S

C

L

L

E

A
x
V

N

L

D

K

A

G

I

I

D

N

L

V

S

H

I

I

A

R

P

E

G

G

E

E

I

V

H

V

A

V

L

V

L

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

L

K

R

I

C

I

L

Y

N

G

L

V

L

T

E

H

D

A

F

D

D

S

E

G

G

E

E

M

I

I

I

W

I

Q

D

G

G

Q

I

R

N

V

L

T

K

M

A

R

K

-

D

-

T

N

N

P

L

A

N

Q

K

A

V

R

R

G

E

L

-

G

E

I

V

G

G

M

M

V

V

F

F

Q

Q

H

R

F

F

S

N

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

L

Q

N

N

N

I

I

A

T

L

L

A

A

M

P

G

M

A

K

A

V

A

R

G

K

T

W

P

P

K

R

Q

E

-

K

L

A

E

E

P

A

K

K

I

A

R

M

E

E

V

L

S

L

Q

D

R

K

Y

V

G

G

M

L

A

K

L

D

E

K

P

A

E
x
H

R

A

L
x
Y

V

P

H

D

S

S

L

L

S

S

I

G

G

G

E

Q

R

A

Q

Q

R

R

V

V

E

A

I

I

I

A

R

R

C

A

L

L

M

A

Q

M

D

E

I

P

R

K

L

I

L

M

I

L

L

F

D

D

E

E

P

P

T

T

S

S

V

A

L

L

T

D

P

P

Q

E

E

M

A

V

D

G

E

E

L

V

F

L

V

S

T

V

L

M

R

K

R

Q

L

L

A

A

A

N

E

E

G

G

C

M

S

T

I

M

L

V

F

V

I

V

S

T

H

H

K

E

L

M

G

G

E

F

V

A

R

R

A

E

L

V

C

G

H

D

S

R

A

V

T

L

V

F

L

M

R

D

G

G

R

Y

V

I

A

I

G

E

H

E

C

G

V

K

P

P

A

E

Q

D

C

L

S

F

D

D

R

R

binding adenosine-5'-diphosphate: F13 (≠ Y23), V18 (≠ A28), S38 (≠ N48), G39 (= G49), S40 (≠ A50), G41 (= G51), K42 (= K52), S43 (= S53), T44 (= T54), H133 (≠ E141), Y135 (≠ L143)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 41% coverage: 14:225/521 of query aligns to 4:213/371 of P68187

query
sites
P68187

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y

W

P

G

G

E

C

V

L

V

A

V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S
x
A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T
x
K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I
x
V

R

N

E

Q

V
|
V

S

A

Q
x
E

R

V

Y

L

G

Q

M

L

A
|
A

L

H

E

L

P

L

E

D

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G
|
G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D
|
D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

A85 (≠ S98) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ T119) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ I127) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (= V130) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ Q132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 3:212/371 of 3puyA

query
sites
3puyA

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E
x
P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I
x
G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D
|
D

E
|
E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y23), V17 (≠ A28), P36 (≠ E47), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (= Q95), R128 (= R142), A132 (≠ S146), S134 (= S148), G135 (≠ I149), G136 (= G150), Q137 (≠ E151), E158 (= E172), H191 (= H204)
binding magnesium ion: S42 (= S53), Q81 (= Q95), D157 (= D171)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 3:212/371 of 3puxA

query
sites
3puxA

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R
|
R

L

K

V

P

H

K

S
x
A

L
|
L

S
|
S

I
x
G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D
|
D

E
|
E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R142), A132 (≠ S146), L133 (= L147), S134 (= S148), Q137 (≠ E151)
binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S148), G135 (≠ I149), G136 (= G150), E158 (= E172), H191 (= H204)
binding magnesium ion: S42 (= S53), Q81 (= Q95), D157 (= D171)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 3:212/371 of 3puwA

query
sites
3puwA

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q
|
Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R
|
R

L

K

V

P

H

K

S
x
A

L

L

S
|
S

I
x
G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E
|
E

P

P

T

L

S

S

V
x
N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R142), A132 (≠ S146), S134 (= S148), Q137 (≠ E151)
binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S148), G135 (≠ I149), G136 (= G150), E158 (= E172), N162 (≠ V176), H191 (= H204)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

2awoA Crystal structure of the adp-mg-bound e. Coli malk (crystallized with adp-mg) (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 3:212/372 of 2awoA

query
sites
2awoA

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G

G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D
|
D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)
binding magnesium ion: S42 (= S53), D157 (= D171)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 3:212/374 of 2awnB

query
sites
2awnB

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R

R

L

K

V

P

H

K

S

A

L

L

S

S

I

G

G

G

E

Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V

N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H

H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding adenosine-5'-diphosphate: W12 (≠ Y23), V17 (≠ A28), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 41% coverage: 14:225/521 of query aligns to 1:210/367 of 1q12A

query
sites
1q12A

L

V

Q

Q

L

L

R

Q

R

N

I

V

S

T

K

K

R

A

Y
x
W

P

G

G

E

C

V

L

V

A
x
V

N

S

D

K

A

D

I

I

D

N

L

L

S

D

I

I

A

H

P

E

G

G

E

E

I

F

H

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

K

R

I

M

I

I

Y

A

G

G

V

L

T

E

H

T

A

I

D

T

S

S

G

G

E

D

M

L

I

F

W

I

Q

G

G

E

Q

K

R

R

V

M

T

N

M

D

R

T

N

P

P

P

A

A

Q

E

A

R

R

-

G

-

L

-

G

G

I

V

G

G

M

M

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

Y

E

P

T

H

L

L

S

S

V

V

A

A

Q

E

N

N

I

M

A

S

L

F

A

G

M

L

G

K

A

L

A

A

A

G

G

A

T

K

P

K

K

E

Q

V

L

I

E

N

P

Q

K

R

I

V

R

N

E

Q

V

V

S

A

Q

E

R

V

Y

L

G

Q

M

L

A

A

L

H

E

L

P

L

E

D

R
|
R

L

K

V

P

H
x
K

S
x
A

L
|
L

S
|
S

I
x
G

G
|
G

E
x
Q

R

R

Q

Q

R

R

V

V

E

A

I

I

I

G

R

R

C

T

L

L

M

V

Q

A

D

E

I

P

R

S

L

V

L

F

I

L

L

L

D

D

E

E

P

P

T

L

S

S

V
x
N

L

L

T

D

P

A

Q

A

E

L

A

R

D

V

E

Q

L

M

F

R

V

I

T

E

L

I

R

S

R

R

L

L

A

H

A

K

E

R

-

L

G

G

C

R

S

T

I

M

L

I

F

Y

I

V

S

T

H
|
H

K

D

L

Q

G

V

E

E

V

A

R

M

A

T

L

L

C

A

H

D

S

K

A

I

T

V

V

V

L

L

R

D

G

A

G

G

R

R

V

V

A

A

binding adenosine-5'-triphosphate: W10 (≠ Y23), V15 (≠ A28), S35 (≠ N48), G36 (= G49), C37 (≠ A50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (= R142), K129 (≠ H145), A130 (≠ S146), L131 (= L147), S132 (= S148), G133 (≠ I149), G134 (= G150), Q135 (≠ E151), N160 (≠ V176), H189 (= H204)

6z67B Ftse structure of streptococcus pneumoniae in complex with amppnp at 2.4 a resolution (see paper)
30% identity, 41% coverage: 14:225/521 of query aligns to 4:218/229 of 6z67B

query
sites
6z67B

L

I

Q

E

L

M

R

R

R

D

I

V

S

V

K

K

R

K

Y
|
Y

P

D

-

N

G
|
G

C
x
T

L

T

A
|
A

N

L

D

R

A

G

I

V

D

S

L

V

S

S

I

V

A

Q

P

P

G

G

E

E

I

F

H

A

A

Y

L

I

L

V

G

G

E

P

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

I

K

R

I

S

I

L

Y

Y

G

R

V

E

T

V

H

K

A

I

D

D

S

K

G

G

E

S

M

L

I

S

W

V

Q

A

G

G

Q

F

R

N

V

L

T

V

M

K

R

I

N

K

P

K

A

K

Q

D

A

V

R

P

G

L

L

L

-

R

G

S

I

V

G

G

M

V

V

V

F

F

Q

Q

H

D

F

Y

S

K

L

L

F

L

E

P

T

K

L

K

S

T

V

V

A

Y

Q

E

N

N

I

I

A

A

L

Y

A

A

M

M

G

E

A

V

A

I

A

G

G

E

T

N

P

R

K

R

Q

N

L

I

E

K

P

R

K

R

I

V

R

M

E

E

V

V

S

L

Q

D

R

L

Y

V

G

G

M

L

A

K

L

H

E

K

P

V

E

R

R

S

L

F

V

P

H

N

S

E

L

L

S

S

I

G

G

G

E

E

R

Q

Q

Q

R

R

V

I

E

A

I

I

I

A

R

R

C

A

L

I

M

V

Q

N

D

N

I

P

R

K

L

V

L

L

I

I

L

A

D

D

E

E

P

P

T

T

S

G

V

N

L

L

T

D

P

P

Q

D

E

N

A

S

D

W

E

E

L

I

F

M

V

N

T

L

L

L

R

E

R

R

L

I

A

N

A

L

E

Q

G

G

C

T

S

T

I

I

L

L

F

M

I

A

S

T

H
|
H

K

N

L

S

G

Q

E

I

V

V

R

N

A

T

L

L

C

R

H

H

S

R

A

V

T

I

V

A

L

I

R

E

G

N

G

G

R

R

V

V

A

V

binding phosphoaminophosphonic acid-adenylate ester: Y13 (= Y23), G16 (= G25), T17 (≠ C26), A19 (= A28), S39 (≠ N48), G40 (= G49), A41 (= A50), G42 (= G51), K43 (= K52), S44 (= S53), T45 (= T54), H197 (= H204)

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
30% identity, 41% coverage: 14:225/521 of query aligns to 4:218/230 of 6z4wA

query
sites
6z4wA

L

I

Q

E

L

M

R

R

R

D

I

V

S

V

K

K

R

K

Y
|
Y

P

D

-
x
N

G

G

C
x
T

L

T

A
|
A

N

L

D

R

A

G

I

V

D

S

L

V

S

S

I

V

A

Q

P

P

G

G

E

E

I

F

H

A

A

Y

L

I

L

V

G

G

E

P

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

F

M

I

K

R

I

S

I

L

Y

Y

G

R

V

E

T

V

H

K

A

I

D

D

S

K

G

G

E

S

M

L

I

S

W

V

Q

A

G

G

Q

F

R

N

V

L

T

V

M

K

R

I

N

K

P

K

A

K

Q

D

A

V

R

P

G

L

L

L

-

R

G

S

I

V

G

G

M

V

V

V

F

F

Q

Q

H

D

F

Y

S

K

L

L

F

L

E

P

T

K

L

K

S

T

V

V

A

Y

Q

E

N

N

I

I

A

A

L

Y

A

A

M

M

G

E

A

V

A

I

A

G

G

E

T

N

P

R

K

R

Q

N

L

I

E

K

P

R

K

R

I

V

R

M

E

E

V

V

S

L

Q

D

R

L

Y

V

G

G

M

L

A

K

L

H

E

K

P

V

E

R

R

S

L

F

V

P

H

N

S

E

L

L

S

S

I

G

G

G

E

E

R

Q

Q

Q

R

R

V

I

E

A

I

I

I

A

R

R

C

A

L

I

M

V

Q

N

D

N

I

P

R

K

L

V

L

L

I

I

L

A

D

D

E

E

P

P

T

T

S

G

V

N

L

L

T

D

P

P

Q

D

E

N

A

S

D

W

E

E

L

I

F

M

V

N

T

L

L

L

R

E

R

R

L

I

A

N

A

L

E

Q

G

G

C

T

S

T

I

I

L

L

F

M

I

A

S

T

H

H

K

N

L

S

G

Q

E

I

V

V

R

N

A

T

L

L

C

R

H

H

S

R

A

V

T

I

V

A

L

I

R

E

G

N

G

G

R

R

V

V

A

V

binding adenosine-5'-diphosphate: Y13 (= Y23), N15 (vs. gap), T17 (≠ C26), A19 (= A28), S39 (≠ N48), G40 (= G49), A41 (= A50), G42 (= G51), K43 (= K52), S44 (= S53), T45 (= T54)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 43% coverage: 12:235/521 of query aligns to 16:237/378 of P69874

query
sites
P69874

P

P

R

L

L

V

Q

Q

L

L

R

A

R

G

I

I

S

R

K

K

R
x
C

Y
x
F

P

D

G

G

C

K

L

E

A

V

N

I

D

P

A

Q

I

L

D

D

L

L

S

T

I

I

A

N

P

N

G

G

E

E

I
x
F

H

L

A

T

L

L

G

G

E

P

N

S

G

G

A
x
C

G

G

K

K

S

T

T

T

L

V

M
x
L

K

R

I

L

I

I

Y

A

G

G

V

L

T

E

H

T

A

V

D

D

S

S

G

G

E

R

M

I

I

M

W
x
L

Q

D

G

N

Q

E

R

D

V

I

T

T

M

-

R

H

N

V

P

P

A

A

Q

E

A

N

R

R

G

Y

L

-

G

-

I

V

G

N

M

T

V

V

F

F

Q

Q

H

S

F

Y

S

A

L

L

F

F

E

P

T

H

L

M

S

T

V

V

A

F

Q

E

N

N

I

V

A

A

L

F

A

G

M

L

G

R

A

M

A

Q

A

K

G

T

T

P

P

A

K

A

Q

E

L

I

E

T

P

P

K

R

I

V

R

M

E

E

V

A

S

L

Q

R

R

M

Y
x
V

G

Q

M

L

A

E

L

T

E

F

P

A

E

Q

R

R

L

K

V

P

H

H

S

Q

L

L

S

S

I

G

G

G

E

Q

R

Q

Q

Q

R

R

V

V

E

A

I

I

I

A

R

R

C

A

L

V

M

V

Q

N

D

K

I

P

R

R

L

L

I

L

L

L

D
|
D

E

E

P

S

T

L

S

S

V

A

L

L

T

D

-

Y

-

K

-

L

-

R

P

K

Q

Q

E

M

A

Q

D

N

E

E

L

L

F

K

V

A

T

L

L

Q

R

R

R

K

L

L

A

-

E

-

G

G

C

I

S

T

I

F

L

V

F

F

I

V

S

T

H

H

K

D

L

Q

G

E

E

E

V

A

R

L

A

T

L

M

C

S

H

D

S

R

A

I

T

V

V

V

L

M

R

R

G

D

G

G

R

R

V

I

A

E

G

Q

H

D

C

G

V

T

P

P

A

R

Q

E

C

I

S

Y

D

E

C26 (≠ R22) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ Y23) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ I41) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A50) mutation to T: Loss of ATPase activity and transport.
L60 (≠ M56) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ W72) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ Y134) mutation to M: Loss of ATPase activity and transport.
D172 (= D171) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1g6hA Crystal structure of the adp conformation of mj1267, an atp- binding cassette of an abc transporter (see paper)
27% identity, 45% coverage: 14:245/521 of query aligns to 5:254/254 of 1g6hA

query
sites
1g6hA

L

L

Q

R

L

T

R

E

R

N

I

I

S

V

K

K

R

Y

Y
x
F

P

G

G

E

C
x
F

L

K

A

A

N

L

D

D

A

G

I

V

D

S

L

I

S

S

I

V

A

N

P

K

G

G

E

D

I

V

H

T

A

L

L

I

G

G

E

P

N
|
N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

K

N

I

V

I

I

Y

T

G

G

V

F

T

L

H

K

A

A

D

D

S

E

G

G

E

R

M

V

I

Y

W

F

Q

E

G

N

Q

K

R

D

V

I

T

T

M

N

R

K

N

E

P

P

A

A

Q

E

A

L

R

Y

G

H

L

Y

G

G

I

I

G

V

M

R

V

T

F

F

Q

Q

H

T

F

P

S

Q

L

P

F

L

E

K

T

E

L

M

S

T

V

V

A

L

Q

E

N

N

I

L

A

L

L

I

A

G

M

E

G

I

A

C

A

P

A

G

G

E

T

S

P

P

-

L

-

N

-

S

-

L

-

F

-

Y

K

K

Q

K

L

W

E

I

P

P

K

K

I

E

R

E

E

E

V

M

S

V

Q

E

R

K

Y

A

G

F

M

K

A

I

L

L

E

E

P

F

E

L

R

K

L

L

V

S

H

H

-

L

-

Y

-

D

-

R

-

K

-

A

-

G

S

E

L

L

S

S

I

G

G

G

E

Q

R

M

Q

K

R

L

V

V

E

E

I

I

I

G

R

R

C

A

L

L

M

M

Q

T

D

N

I

P

R

K

L

M

L

I

I

V

L

M

D

D

E

E

P

P

T

I

S

A

V

G

L

V

T

A

P

P

Q

G

E

L

A

A

D

H

E

D

L

I

F

F

V

N

T

H

L

V

R

L

R

E

L

L

A

K

A

A

E

K

G

G

C

I

S

T

I

F

L

L

F

I

I

I

S

E

H

H

K

R

L

L

G

D

E

I

V

V

R

L

A

N

L

Y

C

I

H

D

S

H

A

L

T

Y

V

V

L

M

R

F

G

N

G

G

R

Q

V

I

A

I

G

A

H

E

C

G

-

R

-

G

-

E

V

I

P

K

A

N

Q

V

C

L

S

S

D

D

R

P

Q

K

L

V

A

V

Q

E

L

I

M

Y

V

I

G

G

E

E

binding adenosine-5'-diphosphate: F14 (≠ Y23), F17 (≠ C26), N39 (= N48), G40 (= G49), S41 (≠ A50), G42 (= G51), K43 (= K52), S44 (= S53), T45 (= T54)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
29% identity, 48% coverage: 13:262/521 of query aligns to 3:263/353 of 1oxvD

query
sites
1oxvD

R

R

L

I

Q

I

L

V

R

K

R

N

I

V

S

S

K

K

R

V

Y
x
F

P

K

G

K

-

G

-

K

C
x
V

L

V

A
|
A

N

L

D

D

A

N

I

V

D

N

L

I

S

N

I

I

A

E

P

N

G

G

E

E

I

R

H

F

A

G

L

I

L

L

G

G

E
x
P

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K