SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
S144 (= S144) modified: 2,3-didehydroalanine (Ser); mutation S->A,T: Complete loss of activity.; mutation to C: No effect.

1gkmA Histidine ammonia-lyase (hal) from pseudomonas putida inhibited with l-cysteine (see paper)
83% identity, 100% coverage: 2:510/510 of query aligns to 1:507/507 of 1gkmA

query
sites
1gkmA

T

T

A

E

L

L

N

T

L

L

I

K

P

P

G

G

Q

T

L

L

S

T

L

L

A

A

Q

Q

L

L

R

R

D

A

V

I

Y

H

Q

A

P

P

V

V

K

R

I

L

T

Q

L

L

D

D

N

A

S

S

A

A

S

A

A

P

Q

A

I

I

E

D

A

A

S

S

V

V

A

A

C

C

V

V

E

E

Q

Q

I

I

L

I

A

A

E

E

N

D

R

R

T

T

A

A

Y
|
Y

G

G

I

I

N

N

T

T

G

G

F

F

G
|
G

L

L

A

A

S

S

T

T

R

R

I

I

A

A

S

S

E

H

D

D

L

L

E

E

N

N

L

L

Q

Q

R

R

S

S

L

L

V

V

L

L

S

S

H
|
H

A

A

G

G

V

I

G

G

E

A

P

P

I

L

S

D

D

D

A

D

L

L

V

V

R

R

L

L

V

I

M

M

V

V

L

L

K

K

V

I

N

N

S

S

L

L

S

S

R

R

G

G

F

F

S

S

G

G

I

I

R

R

V

K

V

V

I

I

D

D

A

A

L

L

I

I

A

A

L

L

I

V

N

N

A

A

E

E

V

V

Y

Y

P

P

H

H

I

I

P

P

L

L

K

K

G

G

S

S

V

V

G

G

A

-

S

-

G
|
G

D

D

L

L

A

A

P

P

L

L

A

A

H

H

M

M

S

S

L

L

V

V

L

L

G

G

E

E

G

G

K

K

A

A

R

R

Y

Y

K

K

G

G

E

Q

W

W

M

L

E

S

A

A

T

T

E

E

A

A

L

L

K

A

V

V

A

A

G

G

L

L

T

E

P

P

L

L

T

T

L

L

A

A

K

K

E

E

G

G

L
|
L

A

A

L

L

N
|
N

G

G

T

T

Q

Q

V

A

S

S

T

T

A

A

Y

Y

A

A

L

L

R

R

G

G

L

L

F

F

E

Y

G

A

E

E

D

D

L

L

F

Y

A

A

G

A

A

A

L

I

A

A

I

C

G

G

M

L

T

S

V

V

E

E

A

A

V

V

L

L

G

G

S

S

R

R

S

S

P

P

F

F

D

D

A

A

R

R

I

I

H

H

A

E

A

A

R

R

G

G

Q

Q

K

R

G

G

Q

Q

I

I

D

D

A

T

A

A

A

C

Y

F

R

R

D

D

L

L

G

G

E

D

R

S

S

S

E

E

V

V

S

S

D

L

S

S

H

H

Q

K

N

N

C

A

E

D

K

K

V

V

Q

Q

D

D

P

P

Y
|
Y

S

S

L

L

R
|
R

C

C

Q

Q

P

P

Q

Q

V

V

M

M

G

G

A

A

C

C

L

L

T

T

Q

Q

F

L

R

R

Q

Q

A

A

E

E

V

V

L

L

A

G

I

I

E

E

A

A

N

N

A

A

V

V

S

S

D

D

N

N

P

P

L

L

V

V

F

F

A

A

E

E

G

G

D

D

V

V

I

I

S

S

G

G

N

N

F
|
F

H

H

A

A

E

E

P

P

V

V

A

A

M

M

A

A

D

D

N

N

M

L

A

A

L

L

A

A

I

I

A

A

E

E

I

I

G

G

S

S

L

L

S

S

E

E

R

R

I

I

S

S

L

L

M

M

D

D

K

K

H

H

M

M

S

S

Q

Q

L

L

P

P

F

F

L

L

V

V

A

E

N

N

G

G

V

V

N

N

S

S

G

G

F

F

M

M

I

I

A

A

Q

Q

V

V

T

T

A

A

L

L

A

A

S

S

E

E

N

N

K

K

A

A

L

L

S

S

H

H

P

P

H

H

S

S

V

V

D

D

S

S

L

L

P

P

T

T

S

S

A

A

N

N

Q

Q

E
|
E

D

D

H

H

V

V

S

S

M

M

A

A

P

P

A

A

G

G

K

K

R

R

L

L

W

W

E

E

M

M

A

A

E

E

N

N

T

T

R

R

G

G

V

V

L

L

A

A

V

I

E

E

W

W

L

L

A

G

A

A

A

C

Q

Q

G

G

L

L

D

D

L

L

R

R

E

K

G

G

L

L

K

K

T

T

S

S

T

A

K

K

L

L

E

E

K

K

A

A

R

R

A

Q

I

A

L

L

R

R

A

S

E

E

V

V

P

A

F

H

Y

Y

E

D

K

R

D

D

R

R

F

F

A

A

P

P

D

D

I

I

N

E

A

K

A

A

S

V

E

E

L

L

A

A

T

K

R

G

C

S

L

L

N

T

E

G

L

L

V

L

S

P

A

A

K

G

L

V

L

L

P

P

S

S

L

L

active site: Y53 (= Y54), G60 (= G61), H83 (= H84), N193 (= N196), Y278 (= Y281), R281 (= R284), F327 (= F330), E412 (= E415)
binding cysteine: G142 (= G145), L189 (= L192), N193 (= N196), F327 (= F330)

P21213 Histidine ammonia-lyase; Histidase; EC 4.3.1.3 from Rattus norvegicus (Rat) (see paper)
44% identity, 95% coverage: 11:493/510 of query aligns to 121:607/657 of P21213

query
sites
P21213

L

L

S

S

L

T

A

E

Q

D

L

L

R

V

D

N

V

L

Y

G

Q

K

Q

G

P

H

V

Y

K

K

I

I

T

K

L

L

D

T

N

S

S

I

A

A

S

E

A

K

Q

K

I

V

E

Q

A

Q

S

S

V

R

A

E

C

V

V

I

E

D

Q

S

I

I

L

I

A

K

E

E

N

R

R

T

T

V

A

V

Y

Y

G

G

I

I

N

T

T

T

G

G

F

F

G

G

L

K

L

F

A

A

S

R

T

T

R

V

I

I

A

P

S

A

E

N

D

K

L

L

E

Q

N

E

L

L

Q

Q

R

V

S

N

L

L

V

V

L

R

S

S

H

H

A

S

G

G

V

V

G

G

E

K

P

P

I

L

S

S

D

P

A

E

L

R

V

C

R

R

L

M

V

L

M

L

V

A

L

L

K

R

V

I

N

N

S

V

L

L

S

A

R

K

G

G

F

Y

S

S

G

G

I

I

R

S

R

L

V

E

V

T

I

L

D

K

A

Q

L

V

I

I

A

E

L

V

I

F

N

N

A

A

E

S

V

C

Y

L

P

S

H

Y

I

V

P

P

L

E

K

K

G

G

S

T

V

V

G

G

A

A

S
|
S

G

G

D

D

L

L

A

A

P

P

L

L

A

S

H

H

M

L

S

A

L

L

V

G

L

L

L

I

G

G

E

E

G

G

K

K

A

M

R

W

Y

S

-

P

K

K

G

S

E

G

W

W

M

A

E

D

A

A

T

K

E

Y

A

V

L

L

K

E

V

A

A

H

G

G

L

L

T

K

P

P

L

I

T

V

L

L

A

K

A

P

K

K

E

E

G

G

L

L

A

A

L

L

L

I

N

N

G

G

T

T

Q

Q

V

M

S

I

T

T

A

S

Y

L

A

G

L

C

R

E

G

A

L

V

F

E

E

R

G

A

E

S

D

A

L

I

F

A

A

R

G

Q

A

A

L

D

A

I

I

V

G

A

M

L

T

T

V

L

E

E

A

V

V

L

L

K

G

G

S

T

R

T

S

K

P

A

F

F

D

D

A

T

R

D

I

I

H

H

A

A

A

V

R

R

G

P

Q

H

K

R

G

G

Q

Q

I

I

D

E

A

V

A

A

A

F

A

R

Y

F

R

R

D

S

L

L

G

D

-

S

-

D

-

H

E

H

R

P

S

S

E

E

V

I

S

A

D

E

S

S

H

H

Q

R

N

F

C

C

E

D

K

R

V

V

Q

Q

D

D

P

A

Y

Y

S

T

L

L

R

R

C

C

Q

C

P

P

Q

Q

V

V

M

H

G

G

A

V

C

V

L

N

T

D

Q

T

F

I

R

A

Q

F

A

V

A

K

E

D

V

I

L

I

A

T

I

T

E

E

A

L

N

N

A

S

V

A

S

T

D

D

N

N

P

P

L

M

V

V

F

F

A

A

A

S

E

R

G

G

D

E

V

T

I

I

S

S

G

G

N

N

F

F

H

H

A

G

E

E

P

Y

V

P

A

A

M

K

A

A

A

L

D

D

N

Y

M

L

A

A

L

I

A

G

I

V

A

H

E

E

I

L

G

A

S

A

L

I

S

S

E

E

R

R

I

I

S

E

L

R

M

L

M

C

D

N

K

P

H

S

M

L

S

S

Q

E

L

L

P

P

P

A

F

F

L

L

V

V

A

A

N

E

G

G

V

L

N

N

S

S

G

G

F

F

M

M

I

I

A

A

Q

H

V

C

T

T

A

A

L

L

A

V

S

S

E

E

N

S

K

K

A

A

L

L

S

C

H

H

P

P

H

S

S

S

V

V

D

D

S

S

L

L

P

S

T

T

S

S

A

A

N

A

Q

T

E

E

D

D

H

H

V

V

S

S

M

M

A

G

P

G

A

W

A

A

G

A

K

R

R

K

L

A

W

L

E

R

M

V

A

I

E

E

N

H

T

V

R

E

G

Q

V

V

L

L

A

A

V

I

E

E

W

L

L

L

A

A

A

C

Q

Q

G

G

L

I

D

E

L

F

R

L

E

R

G

P

L

L

K

K

T

T

S

T

T

T

K

P

L

L

E

E

K

K

A

V

R

Y

A

D

I

L

L

V

R

R

A

S

E

V

V

V

P

R

F

P

Y

W

E

I

K

K

D

D

R

R

F

F

F

M

A

A

P

P

D

D

I

I

N

E

A

A

S

H

E

R

L

L

S254 (= S144) mutation to A: Complete loss of activity.

Q20502 Histidine ammonia-lyase; Histidase; EC 4.3.1.3 from Caenorhabditis elegans (see paper)
42% identity, 92% coverage: 26:493/510 of query aligns to 152:623/677 of Q20502

query
sites
Q20502

I

I

T

Q

L

L

D

S

N

M

S

E

A

S

S

E

A

D

Q

R

I

I

E

R

A

K

S

A

V

R

A

T

C

F

V

L

E

E

Q

K

I

I

L

A

A

S

E

E

N

H

R

R

T

A

A

V

Y

Y

G

G

I

V

N

T

T

T

G

G

F

F

G

G

L

T

L

F

A

S

S

N

T

V

R

T

I

I

A

P

S

P

E

E

D

K

L

L

E

K

N

K

L

L

Q

Q

R

L

S

N

L

L

V

I

L

R

S

S

H

H

A

A

A

T

G

G

V

Y

G

G

E

E

P

P

I

L

S

A

D

P

A

N

L

R

V

A

R

R

L

M

V

L

M

L

V

A

L

L

K

R

V

I

N

N

S

I

L

L

S

A

R

K

G

G

F

H

S

S

G

G

I

I

R

S

R

V

V

E

V

N

I

I

D

K

A

K

L

M

I

I

A

A

L

A

I

F

N

N

A

A

E

F

V

C

Y

V

P

S

H

Y

I

V

P

P

L

Q

K

Q

G

G

S

T

V

V

G

G

A

C

S

S

G

G

D

D

L

L

A

C

P

P

L

L

A

A

H

H

M

L

S

A

L

L

V

G

L

L

G

G

E

E

G

G

K

K

A

M

R

W

Y

S

K

P

G

T

E

T

-

G

W

W

M

Q

E

P

A

A

T

D

E

V

A

V

L

L

K

K

V

K

A

N

G

N

L

L

T

E

P

P

L

L

T

E

L

L

A

G

A

P

K

K

E

E

G

G

L

L

A

A

L

L

L

I

N

N

G

G

T

T

Q

Q

V

M

S

V

T

T

A

A

Y

L

A

G

L

A

R

Y

G

T

L

L

F

E

E

R

G

A

E

H

D

N

L

I

F

A

A

R

G

Q

A

A

L

D

A

V

I

I

G

A

M

L

T

S

V

L

E

D

A

V

V

L

L

K

G

G

S

T

R

T

S

R

P

A

F

Y

D

D

A

P

R

D

I

I

H

H

A

R

A

I

R

R

G

P

Q

H

K

R

G

G

Q

Q

I

N

D

L

A

S

A

A

A

L

A

R

Y

L

R

R

D

A

L

L

G

H

E

S

R

E

-

A

-

N

-

P

S

S

E

Q

V

I

S

A

D

E

S

S

H

H

Q

R

N

N

C

C

E

T

K

K

V

V

Q

Q

D

D

P

A

Y

Y

S

T

L

L

R

R

C

C

Q

V

P

P

Q

Q

V

V

M

H

G

G

A

V

C

V

L

H

T

D

Q

T

F

I

R

E

Q

F

A

V

A

R

E

E

V

I

L

I

A

T

I

T

E

E

A

M

N

N

A

S

V

A

S

T

D

D

N

N

P

P

L

L

V

V

F

F

A

A

A

D

E

R

G

E

D

E

V

I

I

I

S

S

G

G

N

N

F

F

H

H

A

G

E

E

P

Y

V

P

A

A

M

K

A

A

A

L

D

D

N

F

M

L

A

A

L

I

A

A

I

V

A

A

E

E

I

L

G

A

S

Q

L

M

S

S

E

E

R

R

I

L

S

E

L

R

M

L

M

V

D

N

K

K

H

E

M

L

S

S

Q

G

L

L

P

P

P

T

F

F

L

L

V

T

A

P

N

D

G

G

V

L

N

N

S

S

G

G

F

F

M

M

I

T

A

V

Q

Q

V

L

T

C

A

A

A

S

L

L

A

V

S

S

E

E

N

N

K

K

A

V

L

L

S

C

H

H

P

P

H

S

S

S

V

V

D
|
D

S

S

L

I

P

P

T

T

S

S

A

C

N

N

Q

Q

E

E

D

D

H

H

V

V

S

S

M

M

A

G

P

G

A

F

A

A

G

A

K

R

R

K

L

A

W

L

E

T

M

V

A

V

E

E

N

H

T

V

R

E

G

A

V

V

L

L

A

A

V

M

E

E

W

L

L

L

A

A

A

C

Q

Q

G

G

L

I

D

E

L

F

R

L

E

K

G

P

L

L

K

I

T

S

S

T

T

A

K

P

L

L

E

H

K

K

A

I

R

Y

A

Q

I

L

L

V

R

R

A

S

E

V

V

A

P

P

F

P

Y

L

E

N

K

E

D

D

R

R

F

Y

F

M

A

K

P

P

D

E

I

I

N

D

A

A

A

V

S

L

E

E

L

M

L

I

D536 (= D406) mutation to N: In am130; causes strong resistance to nickel and zinc toxicity.

Q0VZ68 Tyrosine 2,3-aminomutase; Tyrosine ammonia-lyase; EC 5.4.3.6; EC 4.3.1.23 from Chondromyces crocatus (see paper)
34% identity, 92% coverage: 10:479/510 of query aligns to 7:495/531 of Q0VZ68

query
sites
Q0VZ68

Q

N

L

L

S

S

L

I

A

Y

Q

D

L

V

R

A

D

D

V

V

Y

C

Q

M

Q

K

P

R

V

A

K

T

I

V

T

E

L

L

D

D

N

P

S

S

A

-

S

-

A

-

Q

Q

I

L

E

E

A

R

S

V

V

A

A

V

C

A

V

H

E

E

Q

R

I

T

L

Q

A

A

-

W

-

G

-

E

E

A

N

Q

R

H

T

P

A

I

Y

Y

G

G

I

V

N

N

T

T

G

G

F
|
F

G

G

L

E

L
|
L

A
x
V

S
x
P

T
x
V

R
x
M

I
|
I

A

P

S

R

E

Q

D

H

L

K

E

R

N

E

L

L

Q

Q

R

E

S

N

L

L

V

I

L
x
R

S
|
S

H
|
H

A
|
A

G

G

V

G

G

G

E

E

P

P

I

F

S

A

D

D

A

D

L

V

V

V

R

R

L

A

V

I

M

M

V

L

L

A

K

R

V

L

N

N

S

C

L

L

S

M

R

K

G

G

F

Y

S

S

G

G

I

A

R

S

R

V

V

E

V

T

I

V

D

K

A

L

L

L

I

A

A

E

L

F

I

I

N

N

A

R

E

G

V

I

Y

H

P

P

H

V

I

I

P

P

L

Q

K

Q

G

G

S

S

V

L

G

G

A

A

S

S

G

G

D

D

L

L

A

S

P

P

L

L

A

S

H

H

M

I

S

A

L

L

V

A

L

L

L

I

G

G

E

E

G

G

K

T

A

V

R

S

Y

F

K

K

G

G

E

Q

W

V

M

R

E

K

A

T

T

G

E

D

A

V

L

L

K

R

V

E

A

E

G

G

L

L

T

K

P

P

L

L

T

E

L

L

A
x
G

A

F

K

K

E

G

G

G

L

L

A

T

L

L

L

I

N

N

G

G

T

T

Q

S

V

A

S

M

T

T

A

G

Y

A

A

A

L

C

R

V

G

A

L

L

F

G

E

R

G

A

E

Y

D

H

L

L

F

F

A

R

G

L

A

A

L

L

A

L

I

A

G

T

G

A

M

D

T

F

V

V

E

Q

A

C

V

L

L

G

G

G

S

S

R

T

S

G

P

P

F

F

D

E

A

E

R

R

I

G

H

H

A

L

A

P

R
x
K

G

N

Q

H

K

S

G

G

Q

Q

I

V

D

I

A

V

A

A

A

R

A

E

Y

I

R

R

D

K

L

L

G

A

E

G

R

-

S

S

E

Q

V

L

S

T

D

S

S

D

H

H

Q

Q

N

D

C

L

E

M

K

K

-

E

-

M

-
x
V

-
x
A

-
x
R

-
x
S

-
x
G

-
x
V

-
x
G

-
x
N

-
x
D

-
x
V

-
x
D

-
x
T

-
x
G

-

V

-

Y

V

L

Q

Q

D

D

P

A

Y

Y

S

T

L

L

R

R

C

A

Q

V

P

P

Q

Q

V

I

M

L

G

G

A

P

C

V

L

L

T

D

Q

T

F

L

R

D

Q

F

A

A

A

R

E

K

V

L

L

I

A

E

I

E

E

E

A

L

N

N

A

S

V

T

S

N

D

D

N

N

P

P

L

L

V

I

F

F

A

D

A

V

E

P

G

E

D

Q

V

T

I

F

S

H

G

G

G

A

N

N

F

F

H

H

A

G

E

Q

P

Y

V

V

A

A

M

M

A

A

A

C

D

D

N

Y

M

L

A

N

L

I

A

A

I

V

A

T

E

E

I

I

G

G

S

V

L

L

S

A

E

E

R

R

R

Q

I

L

S

N

L

R

M

L

M

V

D

D

K
x
P

H

N

M

I

S

N

-

G

Q

K

L

L

P

P

F

F

L

L

V

A

-

S

A

A

N

H

G

S

G

G

V

L

N

L

S
x
C

G

G

F

F

M
x
E

I
x
G

A
x
G

Q
|
Q

V
x
Y

T
x
L

A
|
A

A
x
T

A

S

L

I

A

A

S

S

E

E

N

N

K

L

A

D

L

L

S

A

H

A

P

P

H

S

S

S

V

I

D

K

S

S

L

L

P

P

T

S

S
x
N

A
x
G

N
x
S

Q
x
N

E
x
Q

D
|
D

H
x
V

V

V

S

S

M

M

A

G

P

T

A

T

A

S

G

A

K

R

R

K

L

S

W

L

E

R

M

L

A

C

E

E

N

N

T

V

R

G

G

T

V

I

L

V

A

S

V

T

E

L

W

I

L

A

A

A

A

C

A

N

Q

Q

G

A

L

G

D

H

L

I

R

L

E

G

G

N

L

E

K

R

T

F

S

S

T

P

K

P

L

I

E

R

K

E

A

L

R

H

A

G

I

E

L

L

R

S

A

R

E

S

V

V

P

P

F

L

Y

Y

E

Q

K

D

D

D

F57 (= F60) mutation to Y: Loss of aminomutase activity.
LVPVMI 60:65 (≠ LASTRI 63:68) mutation to MIYMLV: Shift towards ammonia lyase activity.
RSHA 79:82 (≠ LSHA 82:85) mutation to TFLS: Total loss of aminomutase activity.
RSHAA 79:83 (≠ LSHAA 82:86) mutation to YHLAT: Total loss of aminomutase activity.
G184 (≠ A187) mutation to R: Gain of aminomutase activity.
K242 (≠ R245) mutation to R: Gain of aminomutase activity.
275:288 (vs. gap) mutation Missing: Total loss of aminomutase activity.
P377 (≠ K363) mutation to R: No effect.
C396 (≠ S380) mutation to S: No effect.
E399 (≠ M383) mutation to A: Loss of aminomutase activity and increased product racemization. Gain of ammonia-lyase activity.; mutation to K: Loss of aminomutase and ammonia-lyase activity. Higher enantiomeric excess of (R)-beta-tyrosine.; mutation to M: Loss of aminomutase and ammonia-lyase activity.
399:406 (vs. 383:390, 25% identical) mutation to MIAQVTSA: Residual aminomutase activity.
427:433 (vs. 411:417, 14% identical) mutation to SAGREDH: Total loss of aminomutase activity.; mutation to SANQEDH: Total loss of aminomutase activity.

3unvA Pantoea agglomerans phenylalanine aminomutase (see paper)
33% identity, 93% coverage: 21:496/510 of query aligns to 22:510/514 of 3unvA

query
sites
3unvA

Q

H

Q

Q

P

P

V

V

K

-

I

-

T

T

L

L

D

H

N

D

S

E

A

V

S

V

A

N

Q

R

I

V

E

T

A

R

S

S

V

R

A

S

C

I

V

L

E

E

Q

S

I

M

L

V

A

S

E

D

N

E

R

R

T

V

A

I

Y
|
Y

G

G

I

V

N

N

T

T

G

S

F

M

G
|
G

L

G

L

F

A

V

S

N

T

Y

R

I

I

V

A

P

S

I

E

A

D

K

L

A

E

S

N

E

L

L

Q

Q

R

N

S

N

L

L

V

I

L

N

S

A

H
x
V

A

A

A

T

G

N

V

V

G

G

E

K

P

Y

I

F

S

D

D

D

A

T

L

T

V

V

R

R

L

A

V

T

M

M

V

L

L

A

K

R

V

I

N

V

S

S

L

L

S

S

R

R

G

G

F

N

S

S

G

A

I

I

R

S

R

I

V

V

V

N

I

F

D

K

A

K

L

L

I

I

A

E

L

I

I

Y

N

N

A

Q

E

G

V

I

Y

V

P

P

H

C

I

I

P

P

L

E

K

K

G

G

S

S

V

L

G

G

A

-

S

-

G
|
G

D

D

L
|
L

A

G

P

P

L

L

A

A

H

A

M

I

S

A

L

L

V

V

L

C

L

T

G

G

E

Q

G

W

K

K

A

A

R

R

Y

Y

K

Q

G

G

E

E

W

Q

M

M

E

S

A

G

T

A

E

M

A

A

L

L

K

E

V

K

A

A

G

G

L

I

T

S

P

P

L

M

T

E

L

L

A

S

A

F

K

K

E

E

G

G

L

L

A

A

L
|
L

L

I

N
|
N

G

G

T

T

Q

S

V

A

S

M

T

V

A

G

Y

L

A

G

L

V

R

L

G

L

L

Y

F

D

E

E

G

V

E

K

D

R

L

L

F

F

A

D

G

T

A

Y

L

L

A

T

I

V

G

T

G

S

M

L

T

S

V

I

E

E

A

G

V

L

L

H

G

G

S

K

R

T

S

K

P

P

F

F

D

E

A

P

R

A

I

V

H

H

A

R

A

M

R

K

G

P

Q

H

K

Q

G

G

Q

Q

I

L

D

E

A

V

A

A

A

T

Y

I

R

W

D

E

L

T

L

L

G

A

E

D

R

S

S

S

E

L

V

A

S

V

D

N

S

E

H

H

Q

E

-

V

-

E

-

K

-

L

-

I

-

A

-

E

-

M

-

D

-

G

-

L

-

V

-

K

-

A

N

S

C

N

E

H

K

Q

V

I

Q

E

D
|
D

P

A

Y

Y

S
|
S

L

I

R

R

C

C

Q

T

P

P

Q

Q

V

I

M

L

G

G

A

P

C

V

L

A

T

D

Q

T

F

L

R

K

Q

N

A

I

A

K

E

Q

V

T

L

L

A

T

I

N

E

E

A

L

N

N

A

S

V

S

S

N

D

D

N
|
N

P

P

L

L

V

I

F

D

A

Q

A

T

E

T

G

E

D

E

V

V

I

F

S

H

G

N

G
|
G

N

H

F
|
F

H

H

A

G

E

Q

P

Y

V

V

A

S

M

M

A

A

A

M

D

D

N

H

M

L

A

N

L

I

A

A

I

L

A

V

E

T

I

M

G

M

S

N

L

L

S

A

E

N

R

R

I

I

S

D

L

R

M

F

M

M

D

D

K

K

H

S

M

N

S

S

Q

N

-

G

L

L

P

P

F

F

L

L

V

C

A

A

-

E

N

N

G

A

G

G

V

L

N

R

S

L

G

G

F

L

M

M

I

G

A

G

Q

Q

V

F

T

M

A

T

A

A

A

S

L

I

A

T

S

A

E

E

N

S

K

R

A

A

L

S

S

C

H

M

P

P

H

M

S

S

V

I

D

Q

S

S

L

L

P

S

T

T

S

T

A

G

N
x
D

Q

F

E

Q

D

D

H

I

V

V

S

S

M

F

A

G

P

L

A

V

A

A

G

A

K

R

R

R

L

V

W

R

E

E

M

Q

A

L

E

K

N

N

T

L

R

K

G

Y

V

V

L

F

A

S

V

F

E

E

W

L

L

L

A

C

A

A

A

C

Q

Q

G

A

L

V

D

D

L

I

R

R

-

G

-

T

E

A

G

G

L

L

K

S

T

K

S

R

T

T

K

R

-

A

-

L

L

Y

E

D

K

K

A

T

R

R

A

T

I

L

L

-

R

-

A

-

E

-

V

V

P

P

F

Y

Y

L

E

E

K

E

D

D

R

K

F

T

F

I

A

S

P

D

D

Y

I

I

N

E

A

S

A

I

S

A

E

Q

L

T

L

V

A

L

T

T

R

K

active site: Y53 (= Y54), G60 (= G61), V83 (≠ H84), L191 (= L194), D291 (= D279), S294 (= S282), G340 (= G328), D427 (≠ N413)
binding phenylalanine: Y53 (= Y54), G60 (= G61), G142 (= G145), L144 (= L147), N326 (= N314), F342 (= F330)
binding (3S)-3-amino-3-phenylpropanoic acid: Y53 (= Y54), G60 (= G61), G142 (= G145), N193 (= N196), N326 (= N314), F342 (= F330)

Q8GMG0 MIO-dependent tyrosine 2,3-aminomutase; Tyrosine ammonia-lyase; EC 5.4.3.6; EC 4.3.1.23 from Streptomyces globisporus (see 3 papers)
35% identity, 95% coverage: 11:494/510 of query aligns to 20:522/539 of Q8GMG0

query
sites
Q8GMG0

L

L

S

T

L

V

A

E

Q

A

L

V

R

R

D

R

V

V

Y

A

Q

E

P

R

V

A

K

T

I

V

T

D

L

V

D

P

N

A

S

E

A

S

S

I

A

A

Q

K

I

A

E

Q

A

K

S

S

V

R

A

E

C

I

V

F

E

E

Q

G

I

I

L

A

A

E

E

Q

N

N

R

I

T

P

A

I

Y
|
Y

G

G

I

V

N

T

T

T

G

G

F

Y

G

G

L
x
E

L

M

A

I

S

Y

T

M

R

Q

I

V

A

D

S

K

E

S

D

K

L

E

E

V

N

E

L

L

Q

Q

R

T

S

N

L

L

V

V

L

R

S

S

H
|
H

A

S

A

A

G

G

V

V

G

G

E

P

P

L

I

F

S

A

D

E

A

D

L

E

V

A

R

R

L

A

V

I

M

V

V

A

L

A

K

R

V

L

N

N

S

T

L

L

S

A

R

K

G

G

F

H

S

S

G

A

I

V

R

R

R

P

V

I

I

L

D

E

A

R

L

L

I

A

A

Q

L

Y

I

L

N

N

A

E

E

G

V

I

Y

T

P

P

H

A

I

I

P

P

L

E

K

I

G

G

S

S

V

L

G

G

A
|
A

S
|
S

G
|
G

D

D

L

L

A

A

P

P

L

L

A

S

H

H

M

V

S

A

L

S

V

T

L

L

L

I

G

G

E

E

G

G

K

Y

A

V

R

L

Y

R

K

D

G

G

E

R

W

P

M

V

E

E

A

T

T

A

E

Q

A

V

L

L

K

A

V

E

A

R

G

G

L

I

T

E

P

P

L

L

T

E

L

L

A

R

A

F

K

K

E

E

G

G

L

L

A

A

L

L

L

I

N
|
N

G

G

T

T

Q

-

V

-

S

S

T

G

A

M

Y

T

A

G

L

L

R

G

G

S

L

L

F

V

E

V

G

G

E

R

D

A

L

L

F

E

A

Q

G

A

A

Q

L

Q

A

A

-

E

-

I

I

V

G

T

G

A

M

L

T

L

V

I

E

E

A

A

V

V

L

R

G

G

S

S

R

T

S

S

P

P

F

F

D

L

A

A

R

E

I

G

H

H

-

D

A

I

A

A

R

R

G

P

Q

H

K

E

G

G

Q

Q

I

I

D

D

A

T

A

A

A

N

Y

M

R

R

D

A

L

L

L

M

-

R

-

G

-

S

G

G

E

L

R

T

S

V

E

E

V

H

S

A

D

D

S

L

H

R

Q

R

N

E

C

L

E

Q

K

K

-

D

-

K

-

E

-

A

-

G

-

K

-

D

-

V

-

Q

-

R

-

S

-

E

-

I

-
x
Y

V

L

Q

Q

D

K

P

A

Y

Y

S

S

L

L

R
|
R

C

A

Q

I

P

P

Q

Q

V

V

M

V

G

G

A

A

C

V

L

R

T

D

Q

T

F

L

R

Y

Q

H

A

A

A

R

E

H

V

K

L

L

A

R

I

I

E

E

A

L

N

N

A

S

V

A

S

N

D

D

N

N

P

P

L

L

V

F

F

F

A

E

A

G

E

K

G

-

D

E

V

I

I

F

S

H

G

G

G

A

N

N

F

F

H

H

A

G

E

Q

P

P

V

I

A

A

M

F

A

A

A

M

D

D

N

F

M

V

A

T

L

I

A

A

I

L

A

T

E

Q

I

L

G

G

S

V

L

L

S

A

E

E

R

R

R

Q

I

I

S

N

L

R

M

V

M

L

D

N

K

R

H

H

M

L

S

S

Q

Y

-

G

L

L

P

P

P

E

F

F

L

L

V

V

A

S

-

G

N

D

G

P

G

G

V

L

N

H

S

S

G

G

F

F

M

A

I

G

A

A

Q

Q

V
x
Y

T

P

A

A

A

T

A

A

L

L

A

V

S

A

E

E

N

N

K

R

A

T

L

I

S

G

H

-

P

P

H

A

S

S

V

T

D

Q

S

S

L

V

P

P

T

S

S

N

A

G

N

D

Q

N

E

Q

D

D

H

V

V

V

S

S

M

M

A

G

P

L

A

I

A

S

G

A

K

R

R

N

L

A

W

R

E

R

M

V

A

L

E

S

N

N

T

N

R

N

G

K

V

I

L

L

A

A

V

V

E

E

W

Y

L

L

A

A

Q

Q

G

A

L

V

D

D

L

I

R

S

-

G

-

R

-

F

E

D

G

G

L

L

K

S

T

P

S

A

T

A

K

K

L

-

E

-

K

A

A

T

R

Y

A

E

I

A

L

V

R

R

A

R

E

L

V

V

P

P

F

T

Y

L

E

G

K

V

D

D

R

R

F

Y

F

M

A

A

P

D

D

D

I

I

N

E

A

L

A

V

S

A

E

D

L

A

L

L

A

S

Y63 (= Y54) active site, Proton donor/acceptor; mutation to F: Complete loss of activity. It does not affect the over-all structure of the enzyme.
E71 (≠ L62) mutation to A: Despite a decrease in activity, it shows lyase activity over time and still produced some amount of beta-tyrosine.
H93 (= H84) binding ; mutation to F: Complete loss of activity.
A152 (= A143) modified: Crosslink with 154, 5-imidazolinone (Ala-Gly)
S153 (= S144) modified: 2,3-didehydroalanine (Ser)
G154 (= G145) modified: Crosslink with 152, 5-imidazolinone (Ala-Gly)
N205 (= N196) binding
Y303 (vs. gap) mutation to A: Despite a decrease in activity, it shows lyase activity over time and still produced some amount of beta-tyrosine.
R311 (= R284) binding
Y415 (≠ V387) mutation to V: Complete loss of activity.

2rjsA Sgtam bound to substrate mimic (see paper)
35% identity, 95% coverage: 11:494/510 of query aligns to 9:509/526 of 2rjsA

query
sites
2rjsA

L

L

S

T

L

V

A

E

Q

A

L

V

R

R

D

R

V

V

Y

A

Q

E

P

R

V

A

K

T

I

V

T

D

L

V

D

P

N

A

S

E

A

S

S

I

A

A

Q

K

I

A

E

Q

A

K

S

S

V

R

A

E

C

I

V

F

E

E

Q

G

I

I

L

A

A

E

E

Q

N

N

R

I

T

P

A

I

Y
|
Y

G

G

I

V

N

T

T

T

G

G

F

Y

G
|
G

L

E

L

M

A

I

S

Y

T

M

R

Q

I

V

A

D

S

K

E

S

D

K

L

E

E

V

N

E

L

L

Q

Q

R

T

S

N

L

L

V

V

L

R

S

S

H
|
H

A

S

A

A

G

G

V

V

G

G

E

P

P

L

I

F

S

A

D

E

A

D

L

E

V

A

R

R

L

A

V

I

M

V

V

A

L

A

K

R

V

L

N

N

S

T

L

L

S

A

R

K

G

G

F

H

S

S

G

A

I

V

R

R

R

P

V

I

I

L

D

E

A

R

L

L

I

A

A

Q

L

Y

I

L

N

N

A

E

E

G

V

I

Y

T

P

P

H

A

I

I

P

P

L

E

K

I

G

G

S

S

V

L

G

G

A

-

S

-

G
|
G

D

D

L
|
L

A

A

P

P

L

L

A

S

H

H

M

V

S

A

L

S

V

T

L

L

L

I

G

G

E

E

G

G

K

Y

A

V

R

L

Y

R

K

D

G

G

E

R

W

P

M

V

E

E

A

T

T

A

E

Q

A

V

L

L

K

A

V

E

A

R

G

G

L

I

T

E

P

P

L

L

T

E

L

L

A

R

A

F

K

K

E

E

G

G

L

L

A

A

L

L

L

I

N
|
N

G

G

T

T

Q

-

V

-

S

S

T

G

A

M

Y

T

A

G

L

L

R

G

G

S

L

L

F

V

E

V

G

G

E

R

D

A

L

L

F

E

A

Q

G

A

A

Q

L

Q

A

A

-

E

-

I

I

V

G

T

G

A

M

L

T

L

V

I

E

E

A

A

V

V

L

R

G

G

S

S

R

T

S

S

P

P

F

F

D

L

A

A

R

E

I

G

H

H

-

D

A

I

A

A

R

R

G

P

Q

H

K

E

G

G

Q

Q

I

I

D

D

A

T

A

A

A

N

Y

M

R

R

D

A

L

L

L

M

-

R

-

G

-

S

G

G

E

L

R

T

S

V

E

E

V

H

S

A

D

D

S

L

H

R

Q

R

N

E

C

L

E

Q

K

K

-

D

-

K

-

E

-

A

-

G

-

K

-

D

-

V

-

Q

-

R

-

S

-

E

-

I

-

Y

V

L

Q

Q

D

K

P

A

Y
|
Y

S

S

L

L

R
|
R

C

A

Q

I

P

P

Q

Q

V

V

M

V

G

G

A

A

C

V

L

R

T

D

Q

T

F

L

R

Y

Q

H

A

A

A

R

E

H

V

K

L

L

A

R

I

I

E

E

A

L

N

N

A

S

V

A

S

N

D

D

N

N

P

P

L

L

V

F

F

F

A

E

A

G

E

K

G

-

D

E

V

I

I

F

S

H

G

G

G

A

N

N

F
|
F

H

H

A

G

E

Q

P

P

V

I

A

A

M

F

A

A

A

M

D

D

N

F

M

V

A

T

L

I

A

A

I

L

A

T

E

Q

I

L

G

G

S

V

L

L

S

A

E

E

R

R

R

Q

I

I

S

N

L

R

M

V

M

L

D

N

K

R

H

H

M

L

S

S

Q

Y

-

G

L

L

P

P

P

E

F

F

L

L

V

V

A

S

-

G

N

D

G

P

G

G

V

L

N

H

S

S

G

G

F

F

M

A

I

G

A

A

Q

Q

V

Y

T

P

A

A

A

T

A

A

L

L

A

V

S

A

E

E

N

N

K

R

A

T

L

I

S

G

H

-

P

P

H

A

S

S

V

T

D

Q

S

S

L

V

P

P

T

S

S

N

A

G

N

D

Q

N

E
x
Q

D

D

H

V

V

V

S

S

M

M

A

G

P

L

A

I

A

S

G

A

K

R

R

N

L

A

W

R

E

R

M

V

A

L

E

S

N

N

T

N

R

N

G

K

V

I

L

L

A

A

V

V

E

E

W

Y

L

L

A

A

Q

Q

G

A

L

V

D

D

L

I

R

S

-

G

-

R

-

F

E

D

G

G

L

L

K

S

T

P

S

A

T

A

K

K

L

-

E

-

K

A

A

T

R

Y

A

E

I

A

L

V

R

R

A

R

E

L

V

V

P

P

F

T

Y

L

E

G

K

V

D

D

R

R

F

Y

F

M

A

A

P

D

D

D

I

I

N

E

A

L

A

V

S

A

E

D

L

A

L

L

A

S

active site: Y52 (= Y54), G59 (= G61), H82 (= H84), N192 (= N196), Y295 (= Y281), R298 (= R284), F343 (= F330), Q429 (≠ E415)
binding (3R)-3-amino-2,2-difluoro-3-(4-methoxyphenyl)propanoic acid: Y52 (= Y54), G59 (= G61), H82 (= H84), G141 (= G145), L143 (= L147), N192 (= N196), Y295 (= Y281), R298 (= R284), F343 (= F330), Q429 (≠ E415)

2rjrA Substrate mimic bound to sgtam (see paper)
35% identity, 95% coverage: 11:494/510 of query aligns to 9:509/526 of 2rjrA

query
sites
2rjrA

L

L

S

T

L

V

A

E

Q

A

L

V

R

R

D

R

V

V

Y

A

Q

E

P

R

V

A

K

T

I

V

T

D

L

V

D

P

N

A

S

E

A

S

S

I

A

A

Q

K

I

A

E

Q

A

K

S

S

V

R

A

E

C

I

V

F

E

E

Q

G

I

I

L

A

A

E

E

Q

N

N

R

I

T

P

A

I

Y
|
Y

G

G

I

V

N

T

T

T

G

G

F

Y

G
|
G

L

E

L

M

A

I

S

Y

T

M

R

Q

I

V

A

D

S

K

E

S

D

K

L

E

E

V

N

E

L

L

Q

Q

R

T

S

N

L

L

V

V

L

R

S

S

H
|
H

A

S

A

A

G

G

V

V

G

G

E

P

P

L

I

F

S

A

D

E

A

D

L

E

V

A

R

R

L

A

V

I

M

V

V

A

L

A

K

R

V

L

N

N

S

T

L

L

S

A

R

K

G

G

F

H

S

S

G

A

I

V

R

R

R

P

V

I

I

L

D

E

A

R

L

L

I

A

A

Q

L

Y

I

L

N

N

A

E

E

G

V

I

Y

T

P

P

H

A

I

I

P

P

L

E

K

I

G

G

S

S

V

L

G

G

A

-

S

-

G
|
G

D

D

L
|
L

A

A

P

P

L

L

A

S

H

H

M

V

S

A

L

S

V

T

L

L

L

I

G

G

E

E

G

G

K

Y

A

V

R

L

Y

R

K

D

G

G

E

R

W

P

M

V

E

E

A

T

T

A

E

Q

A

V

L

L

K

A

V

E

A

R

G

G

L

I

T

E

P

P

L

L

T

E

L

L

A

R

A

F

K

K

E

E

G

G

L

L

A

A

L

L

L

I

N
|
N

G

G

T

T

Q

-

V

-

S

S

T

G

A

M

Y

T

A

G

L

L

R

G

G

S

L

L

F

V

E

V

G

G

E

R

D

A

L

L

F

E

A

Q

G

A

A

Q

L

Q

A

A

-

E

-

I

I

V

G

T

G

A

M

L

T

L

V

I

E

E

A

A

V

V

L

R

G

G

S

S

R

T

S

S

P

P

F

F

D

L

A

A

R

E

I

G

H

H

-

D

A

I

A

A

R

R

G

P

Q

H

K

E

G

G

Q

Q

I

I

D

D

A

T

A

A

A

N

Y

M

R

R

D

A

L

L

L

M

-

R

-

G

-

S

G

G

E

L

R

T

S

V

E

E

V

H

S

A

D

D

S

L

H

R

Q

R

N

E

C

L

E

Q

K

K

-

D

-

K

-

E

-

A

-

G

-

K

-

D

-

V

-

Q

-

R

-

S

-

E

-

I

-

Y

V

L

Q

Q

D

K

P

A

Y
|
Y

S

S

L

L

R
|
R

C

A

Q

I

P

P

Q

Q

V

V

M

V

G

G

A

A

C

V

L

R

T

D

Q

T

F

L

R

Y

Q

H

A

A

A

R

E

H

V

K

L

L

A

R

I

I

E

E

A

L

N

N

A

S

V

A

S

N

D

D

N

N

P

P

L

L

V

F

F

F

A

E

A

G

E

K

G

-

D

E

V

I

I

F

S

H

G

G

G

A

N

N

F
|
F

H

H

A

G

E

Q

P

P

V

I

A

A

M

F

A

A

A

M

D

D

N

F

M

V

A

T

L

I

A

A

I

L

A

T

E

Q

I

L

G

G

S

V

L

L

S

A

E

E

R

R

R

Q

I

I

S

N

L

R

M

V

M

L

D

N

K

R

H

H

M

L

S

S

Q

Y

-

G

L

L

P

P

P

E

F

F

L

L

V

V

A

S

-

G

N

D

G

P

G

G

V

L

N

H

S

S

G

G

F

F

M

A

I

G

A

A

Q

Q

V

Y

T

P

A

A

A

T

A

A

L

L

A

V

S

A

E

E

N

N

K

R

A

T

L

I

S

G

H

-

P

P

H

A

S

S

V

T

D

Q

S

S

L

V

P

P

T

S

S

N

A

G

N

D

Q

N

E
x
Q

D

D

H

V

V

V

S

S

M

M

A

G

P

L

A

I

A

S

G

A

K

R

R

N

L

A

W

R

E

R

M

V

A

L

E

S

N

N

T

N

R

N

G

K

V

I

L

L

A

A

V

V

E

E

W

Y

L

L

A

A

Q

Q

G

A

L

V

D

D

L

I

R

S

-

G

-

R

-

F

E

D

G

G

L

L

K

S

T

P

S

A

T

A

K

K

L

-

E

-

K

A

A

T

R

Y

A

E

I

A

L

V

R

R

A

R

E

L

V

V

P

P

F

T

Y

L

E

G

K

V

D

D

R

R

F

Y

F

M

A

A

P

D

D

D

I

I

N

E

A

L

A

V

S

A

E

D

L

A

L

L

A

S

active site: Y52 (= Y54), G59 (= G61), H82 (= H84), N192 (= N196), Y295 (= Y281), R298 (= R284), F343 (= F330), Q429 (≠ E415)
binding (2S,3S)-3-(4-fluorophenyl)-2,3-dihydroxypropanoic acid: Y52 (= Y54), G59 (= G61), H82 (= H84), G141 (= G145), L143 (= L147), N192 (= N196), F343 (= F330), Q429 (≠ E415)

2qveA Crystal structure of sgtam bound to mechanism based inhibitor (see paper)
35% identity, 95% coverage: 11:494/510 of query aligns to 9:509/526 of 2qveA

query
sites
2qveA

L

L

S

T

L

V

A

E

Q

A

L

V

R

R

D

R

V

V

Y

A

Q

E

P

R

V

A

K

T

I

V

T

D

L

V

D

P

N

A

S

E

A

S

S

I

A

A

Q

K

I

A

E

Q

A

K

S

S

V

R

A

E

C

I

V

F

E

E

Q

G

I

I

L

A

A

E

E

Q

N

N

R

I

T

P

A

I

Y
|
Y

G

G

I

V

N

T

T

T

G

G

F

Y

G
|
G

L

E

L

M

A

I

S

Y

T

M

R

Q

I

V

A

D

S

K

E

S

D

K

L

E

E

V

N

E

L

L

Q

Q

R

T

S

N

L

L

V

V

L

R