SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_1584 Histidine ABC transporter, permease protein (TC 3.A.1) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

B5Z7I3 Ergothioneine transport permease/ergothioneine binding protein EgtU from Helicobacter pylori (strain G27) (see paper)
28% identity, 67% coverage: 90:280/283 of query aligns to 55:242/553 of B5Z7I3

query
sites
B5Z7I3

D

D

K

L

L

L

M

A

Q

Q

T

H

L

F

A

I

L

I

M

V

M

A

V

L

A

S

T

G

V

F

I

L

S

V

V

L

L

V

I

F

G

G

I

V

P

L

L

I

G

G

I

V

L

F

S

A

A

F

R

Y

S

N

N

S

R

K

L

A

R

R

S

A

V

F

L

L

M

L

P

P

L

V

L

V

D

N

I

F

M

L

Q

Y

T

T

M

I

P

P

S

S

-

L

-

A

-

L

F

F

V

A

Y

L

L

F

I

I

P

P

V

V

L

I

M

-

L

-

F

-

G

G

L

V

G

G

K

L

V

K

P

N

A

A

I

L

F

L

A

V

T

L

V

V

I

L

Y

Y

A

G

A

L

P

L

P

P

L

I

I

V

R

Y

L

S

T

T

D

Y

L

N

G

A

I

L

R

K

Q

E

V

V

D

R

G

E

E

E

V

V

M

I

E

K

A

A

I

A

N

I

A

G

F

L

G

G

A

C

N

N

R

P

W

K

Q

E

Q

L

L

F

F

F

G

R

V

V

Q

H

L

F

P

L

L

L

A

A

L

I

P

P

S

Q

I

I

M

L

A

A

G

G

I

L

N

R

Q

I

T

A

T

V

M

V

M

M

A

L

L

V

S

A

M

M

V

A

V

G

I

I

A

G

S

A

M

L

I

I

G

G

A

A

R

G

G

G

L

L

G

G

E

Q

D

A

V

I

L

F

V

R

G

G

I

L

Q

N

T

T

L

Q

N

N

V

T

G

T

R

L

G

L

L

V

E

A

A

G

G

S

L

L

A

I

I

I

V

A

I

L

L

F

A

S

V

V

V

L

I

A

D

D

R

K

I

F

T

V

Q

S

A

V

Y

F

G

-

R

-

P

-

R

Q

H

H

E

E

Sites not aligning to the query:

390 Y→A: Disrupts EGT binding.; Y→F: Retains EGT binding.
454 R→A: Retains EGT binding.; R→E: Disrupts EGT binding.

Query Sequence

>Pf1N1B4_1584 Histidine ABC transporter, permease protein (TC 3.A.1)
MFPESFTFSIADWVNSWVDTLVTNYGDVFRSISDTLLWAIVNLEGLLRAAPWWLMLAIVA
GVAWHATRKVVTTSVIVGLLFLVGAVGLWDKLMQTLALMMVATVISVLIGIPLGILSARS
NRLRSVLMPLLDIMQTMPSFVYLIPVLMLFGLGKVPAIFATVIYAAPPLIRLTDLGIRQV
DGEVMEAINAFGANRWQQLFGVQLPLALPSIMAGINQTTMMALSMVVIASMIGARGLGED
VLVGIQTLNVGRGLEAGLAIVILAVVIDRITQAYGRPRHEVSK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory