SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_2033 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2033 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 51% coverage: 24:245/432 of query aligns to 64:299/587 of P25297

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P25297

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K298 (≠ A244) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Sites not aligning to the query:

6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
22% identity, 70% coverage: 12:315/432 of query aligns to 20:315/444 of Q8NLB7

query
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Q8NLB7

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D54 (≠ V44) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (≠ A47) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ L92) mutation to A: Loss of transport activity.
W309 (≠ A309) mutation to V: Loss of transport activity.
D312 (= D312) mutation to A: Loss of transport activity.
R313 (= R313) mutation to A: Loss of transport activity.

Sites not aligning to the query:

317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

P77589 3-(3-hydroxy-phenyl)propionate transporter; 3HPP transporter; 3-(3-hydroxy-phenyl)propionate:H(+) symporter; 3HPP:H(+) symporter from Escherichia coli (strain K12) (see paper)
25% identity, 58% coverage: 63:314/432 of query aligns to 47:277/403 of P77589

query
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P77589

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D75 (= D91) mutation D->A,E: Lack of 3HPP transport activity.
A272 (= A309) mutation to H: 30% increase in 3HPP transport activity.
K276 (≠ R313) mutation to D: Lack of 3HPP transport activity.

Sites not aligning to the query:

27 E→A: Lack of 3HPP transport activity.; E→D: Slight decrease in 3HPP transport activity.

Query Sequence

>Pf1N1B4_2033 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2033
MTTAPSSIAQPTQSTRPLTRNDYKTLSLSALGGALEFYDFIIFVFFATVVGKLFFPADMP
EWLRLMQTFGIFAAGYLARPLGGIVMAHFGDLLGRKKMFTLSIFMMAVPTLIMGLLPTYA
QIGMWAPILLLLMRVIQGAAIGGEVPGAWVFVSEHVPQRHIGYACGTLTSGLTAGILLGS
LVATAINSIYTPVEVSDYAWRIPFLLGGVFGLFSVYLRRWLHETPVFAELQLRKALAEEV
PLRAVLRDHRGAILISMLLTWLLSAAIVVLILMTPTVLQTVYHFAPTTALQSNSVAIVLL
SIGCIIAGALADRFGAGRVFVFGCAALLVSSWTFYHSLAEHPDWLFPMYAITGLLVGTIG
AVPYVMVKAFPPVVRFSGLSFSYNVAYAIFGGLTPMIVSLLLKESPMGPAYYVAVLCGVG
ILVGAYLWKKGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory