SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_2263 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2263 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
29% identity, 51% coverage: 62:213/296 of query aligns to 87:236/296 of P68183

query
sites
P68183

R

N

S

S

L

V

E

K

I

V

A

A

C

G

Y

I

T

S

A

A

I

I

A

G

A

I

V

V

A

A

L

L

G

S

T

T

L

T

A

C

A

A

F

Y

V

A

L

F

T

A

R

R

I

M

T

-

H

R

F

F

K

P

G

G

R

K

-

A

T

T

L

L

F

L

G

K

G

G

L

M

V

L

T

I

A

F

P

Q

L

M

V

-

M

F

P

P

E

A

V

V

I

L

T

S

G

L

L

V

S

A

L

L

L

Y

L

A

L

L

F

F

V

D

A

R

M

L

A

G

Q

E

-

Y

-

I

-

P

M

F

I

I

G

G

W

L

P

N

Q

T

E

H

R

G

G

G

I

V

V

I

T

-

I

-

W

-

I

F

A

A

H

Y

T

L

T

G

F

G

C

I

A

A

A

L

Y

H

V

V

A

W

V

T

V

I

V

-

S

K

A

G

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Y

L

F

R

E

E

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D

D

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S

S

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E

E

E
|
E

A

A

A
|
A

M

A

D

L

L

D

G
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G

A

A

R

T

P

P

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W

K

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A

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F

F

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P
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P

M

L

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A

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P

P

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L

A

A

A

V

G

V

G

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M

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M

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F

F

A

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L

E190 (= E167) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A169) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G173) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P186) mutation to A: No effect.

8hpsB ABC transporter, permease protein SugB (see paper)
26% identity, 54% coverage: 47:206/296 of query aligns to 52:204/264 of 8hpsB

query
sites
8hpsB

Y

Y

V

K

G

A

L

I

M

F

D

S

N

G

T

D

Q

A

L

F

M

T

G

S

S

A

V

L

V

F

R

N

S

S

L

I

E

G

I

I

A

G

C

L

Y

I

T

T

A

T

I

I

A

I

A

A

V

V

A

V

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I

G

G

T

G

L

M

A

A

A

A

F

Y

V

A

L

V

T

A

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R

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L

T

-

H

Q

F

F

K

P

G

G

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K

T

Q

L

L

F

L

G

I

G

G

L

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V

A

T

L

A

L

P

I

L

A

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M

M

F

P

P

E
x
H

V

I

I

-

T

-

G

-

L

-

S

S

L

L

L

V

L

T

L

P

F

I

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F

A

N

M

M

A

W

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R

M

G

I

I

G

G

-

L

-

F

-

D

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W

P

P

Q

-

E

-

R

-

G

G

I

L

V

I

T

-

I

-

W

-

I

I

A

P

H

Y

T

I

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T

F

F

C

A

A

L

A

P

Y

L

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A

A

I

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Y

V

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A

A

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F

R

R

E

E

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P

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D

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L

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E

E

K

A

A

A

A

M

K

D

M

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D

G

G

A

A

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P

W

A

K

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V

A

F

F

F

R

L

K

I

V

T

I

I

A

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P

M

L

I

A

A

A

P

P

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A

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G

A

G

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M

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M

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F

A

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x
F

S

A

L

W

D

N

D

D

binding alpha-D-glucopyranose: H113 (≠ E109), F200 (≠ L202)

Query Sequence

>Pf1N1B4_2263 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2263
MKRFRFSSFMLVAGLLFIYAPMLILVIYSFNASKLVTVWGGWSIKWYVGLMDNTQLMGSV
VRSLEIACYTAIAAVALGTLAAFVLTRITHFKGRTLFGGLVTAPLVMPEVITGLSLLLLF
VAMAQMIGWPQERGIVTIWIAHTTFCAAYVAVVVSARLRELDLSIEEAAMDLGARPWKVF
FLITIPMIAPSLAAGGMMSFALSLDDLVLASFVSGPGSTTLPMEVFSAVRLGVKPEINAV
ASLILLAVSLVTFLVWYFSRRAEEIRKRAIQQAIEEGAADSWKQPDVRRAQAPEAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory