SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_2282 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2282 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
25% identity, 94% coverage: 2:207/218 of query aligns to 180:369/409 of O53289

query
sites
O53289

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D185 (= D7) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ L8) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D9) mutation to N: Decreases enzymatic activity by 15%.
S188 (≠ N10) mutation to A: No effect on enzymatic activity.
S273 (≠ T111) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K156) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D179) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D183) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
25% identity, 94% coverage: 2:207/218 of query aligns to 178:367/396 of 5jlpA

query
sites
5jlpA

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active site: D183 (= D7), V184 (≠ L8), D185 (= D9), G272 (≠ A112), K316 (= K156), D343 (= D183)
binding magnesium ion: D183 (= D7), D339 (= D179)

Sites not aligning to the query:

binding serine: 13, 14

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
25% identity, 93% coverage: 2:203/218 of query aligns to 178:363/396 of 8a21A

query
sites
8a21A

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binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)
binding 4-phenyl-1h-imidazole: D185 (= D9), E192 (≠ D16), V193 (≠ S17), I194 (≠ D18), T211 (≠ A35), M215 (≠ R39), F221 (= F60), R228 (= R67), G273 (≠ T113)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
25% identity, 93% coverage: 2:203/218 of query aligns to 178:363/396 of 8a1zA

query
sites
8a1zA

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binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D9), E192 (≠ D16), M215 (≠ R39), F221 (= F60), L225 (≠ V64), R228 (= R67), G272 (≠ A112), F274 (≠ N114), D339 (= D179)
binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D179)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
25% identity, 93% coverage: 2:203/218 of query aligns to 182:367/411 of A0QJI1

query
sites
A0QJI1

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D187 (= D7) binding
D189 (= D9) binding
D343 (= D179) binding

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
22% identity, 94% coverage: 2:206/218 of query aligns to 4:192/209 of 1l7nA

query
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1l7nA

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K

E

N

A

R

G

G

D

Y

K

V

L

V

V

A

I

V

T
x
S

A
x
G

T

G

N

F

R

D

F

I

V

A

T

V

G

N

P

K

I

I

A

K

T

E

R

K

L

L

G

G

V

L

E

D

T

Y

L

A

I

F

A

A

T

N

E

R

C

L

E

I

M

V

I

K

D

D

G

G

R

K

Y

L

T

T

G

G

R

D

S

V

T

E

D

G

V

E

P

V

C

L

F

K

R

E

E

N

G

A

K
|
K

V

G

T

E

R

I

L

L

N

E

R

K

W

I

L

A

D

K

E

I

T

E

G

G

H

I

N

N

L

L

E

E

D

D

S

T

Y

V

F

A

Y

V

S

G

D
|
D

S

G

M

A

N
|
N

D
|
D

L

I

S

S

L

M

L

F

E

K

Q

K

V

A

A

G

N

L

P

K

I

I

A

A

V

F

D

C

P

A

D

K

P

P

N

I

L

L

R

K

A

E

E

K

A

A

active site: D9 (= D7), F10 (≠ L8), D11 (= D9), G98 (≠ A112), K142 (= K156), D169 (= D183)
binding aluminum fluoride: D9 (= D7), F10 (≠ L8), D11 (= D9), S97 (≠ T111), K142 (= K156)
binding tetrafluoroaluminate ion: D9 (= D7), F10 (≠ L8), D11 (= D9), S97 (≠ T111), G98 (≠ A112), K142 (= K156), N168 (= N182)
binding magnesium ion: D9 (= D7), D11 (= D9), D165 (= D179)

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
22% identity, 94% coverage: 2:206/218 of query aligns to 5:193/210 of 1f5sA

query
sites
1f5sA

R

K

L

L

A

I

L

L

F

F

D
|
D

L
x
F

D
|
D

N

S

T

T

L

L

L

V

G

N

D

E

S

T

D

-

H

-

A

-

W

-

G

-

D

-

Y

-

L

-

C

-

E

-

R

-

G

-

F

-

L

I

D

D

A

E

V

I

A

A

Y

R

K

E

A

A

R

G

N

V

D

E

E

E

-

E

-

V

-

K

F

I

Y

T

Q

K

D

E

Y

A

L

M

A

E

G

G

K

K

L

L

D

N

N

F

D

E

A

Q

Y

S

L

L

N

-

F

-

C

-

L

-

E

-

V

-

L

-

G

-

R

R

T

K

E

R

M

V

A

S

T

L

L

L

D

K

Q

D

W

L

H

P

L

I

D

E

Y

K

M

V

R

E

D

K

C

A

I

I

E

K

P

R

I

I

V

T

L

P

P

T

K

E

G

G

I

A

E

E

L

E

L

T

A

I

K

K

H

E

-

L

R

K

E

N

A

R

G

G

D

Y

K

V

L

V

V

A

I

V

T
x
S

A
x
G

T

G

N

F

R

D

F

I

V

A

T

V

G

N

P

K

I

I

A

K

T

E

R

K

L

L

G

G

V

L

E

D

T

Y

L

A

I

F

A

A

T

N

E

R

C

L

E

I

M

V

I

K

D

D

G

G

R

K

Y

L

T

T

G

G

R

D

S

V

T

E

D

G

V

E

P

V

C

L

F

K

R

E

E

N

G

A

K
|
K

V

G

T

E

R

I

L

L

N

E

R

K

W

I

L

A

D

K

E

I

T

E

G

G

H

I

N

N

L

L

E

E

D

D

S

T

Y

V

F

A

Y

V

S

G

D
|
D

S

G

M

A

N

N

D
|
D

L

I

S

S

L

M

L

F

E

K

Q

K

V

A

A

G

N

L

P

K

I

I

A

A

V

F

D

C

P

A

D

K

P

P

N

I

L

L

R

K

A

E

E

K

A

A

active site: D10 (= D7), F11 (≠ L8), D12 (= D9), G99 (≠ A112), K143 (= K156), D170 (= D183)
binding magnesium ion: D10 (= D7), D12 (= D9), D166 (= D179)
binding phosphate ion: D10 (= D7), F11 (≠ L8), D12 (= D9), S98 (≠ T111), G99 (≠ A112), K143 (= K156)

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
22% identity, 94% coverage: 2:206/218 of query aligns to 6:194/211 of Q58989

query
sites
Q58989

R

K

L

L

A

I

L

L

F

F

D
|
D

L

F

D
|
D

N

S

T

T

L

L

L

V

G

N

D
x
E

S

T

D

-

H

-

A

-

W

-

G

-

D

-

Y

-

L

-

C

-

E

-

R

-

G

-

F

-

L

I

D

D

A

E

V

I

A

A

Y

R

K

E

A

A

R

G

N

V

D

E

E

E

-

E

-

V

-

K

F

I

Y

T

Q

K

D

E

Y

A

L

M

A

E

G

G

K

K

L

L

D

N

N

F

D

E

A

Q

Y

S

L

L

N

-

F

-

C

-

L

-

E

-

V

-

L

-

G

-

R

R

T

K

E
x
R

M

V

A

S

T

L

L

L

D

K

Q

D

W

L

H

P

L

I

D

E

Y

K

M

V

R

E

D

K

C

A

I

I

E

K

P

R

I

I

V

T

L

P

P

T

K

E

G

G

I

A

E

E

L

E

L

T

A

I

K

K

H

E

-

L

R

K

E

N

A

R

G

G

D

Y

K

V

L

V

V

A

I

V

T
x
S

A
x
G

T

G

N

F

R

D

F

I

V

A

T

V

G

N

P

K

I

I

A

K

T

E

R

K

L

L

G

G

V

L

E

D

T

Y

L

A

I

F

A

A

T

N

E

R

C

L

E

I

M

V

I

K

D

D

G

G

R

K

Y

L

T

T

G

G

R

D

S

V

T

E

D

G

V

E

P

V

C

L

F

K

R

E

E

N

G

A

K
|
K

V

G

T

E

R

I

L

L

N

E

R

K

W

I

L

A

D

K

E

I

T

E

G

G

H

I

N

N

L

L

E

E

D

D

S

T

Y

V

F

A

Y

V

S

G

D
|
D

S

G

M

A

N
|
N

D

D

L

I

S

S

L

M

L

F

E

K

Q

K

V

A

A

G

N

L

P

K

I

I

A

A

V

F

D

C

P

A

D

K

P

P

N

I

L

L

R

K

A

E

E

K

A

A

D11 (= D7) active site, Nucleophile; binding ; mutation to N: Loss of activity.
D13 (= D9) active site, Proton donor; binding
E20 (≠ D16) binding
R56 (≠ E69) binding
SG 99:100 (≠ TA 111:112) binding
K144 (= K156) binding
D167 (= D179) binding
N170 (= N182) binding

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
22% identity, 94% coverage: 2:206/218 of query aligns to 3:191/208 of 1l7pA

query
sites
1l7pA

R

K

L

L

A

I

L

L

F

F

D
x
N

L
x
F

D
|
D

N

S

T

T

L

L

L

V

G

N

D
x
E

S

T

D

-

H

-

A

-

W

-

G

-

D

-

Y

-

L

-

C

-

E

-

R

-

G

-

F

-

L

I

D

D

A

E

V

I

A

A

Y

R

K

E

A

A

R

G

N

V

D

E

E

E

-

E

-

V

-

K

F

I

Y

T

Q

K

D

E

Y

A

L
x
M

A

E

G

G

K

K

L

L

D

N

N
x
F

D

E

A

Q

Y

S

L

L

N

-

F

-

C

-

L

-

E

-

V

-

L

-

G

-

R

R

T

K

E
x
R

M

V

A

S

T

L

L

L

D

K

Q

D

W

L

H

P

L

I

D

E

Y

K

M

V

R

E

D

K

C

A

I

I

E

K

P

R

I

I

V

T

L

P

P

T

K

E

G

G

I

A

E

E

L

E

L

T

A

I

K

K

H

E

-

L

R

K

E

N

A

R

G

G

D

Y

K

V

L

V

V

A

I

V

T
x
S

A
x
G

T

G

N

F

R

D

F

I

V

A

T

V

G

N

P

K

I

I

A

K

T

E

R

K

L

L

G

G

V

L

E

D

T

Y

L

A

I

F

A

A

T

N

E

R

C

L

E

I

M

V

I

K

D

D

G

G

R

K

Y

L

T

T

G

G

R

D

S

V

T

E

D

G

V

E

P

V

C

L

F

K

R

E

E

N

G

A

K
|
K

V

G

T

E

R

I

L

L

N

E

R

K

W

I

L

A

D

K

E

I

T

E

G

G

H

I

N

N

L

L

E

E

D

D

S

T

Y

V

F

A

Y

V

S

G

D

D

S

G

M

A

N

N

D
|
D

L

I

S

S

L

M

L

F

E

K

Q

K

V

A

A

G

N

L

P

K

I

I

A

A

V

F

D

C

P

A

D

K

P

P

N

I

L

L

R

K

A

E

E

K

A

A

active site: N8 (≠ D7), F9 (≠ L8), D10 (= D9), G97 (≠ A112), K141 (= K156), D168 (= D183)
binding phosphoserine: N8 (≠ D7), F9 (≠ L8), D10 (= D9), E17 (≠ D16), M40 (≠ L48), F46 (≠ N54), R53 (≠ E69), S96 (≠ T111), G97 (≠ A112), K141 (= K156)

Query Sequence

>Pf1N1B4_2282 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2282
MRLALFDLDNTLLGGDSDHAWGDYLCERGFLDAVAYKARNDEFYQDYLAGKLDNDAYLNF
CLEVLGRTEMATLDQWHLDYMRDCIEPIVLPKGIELLAKHREAGDKLVIITATNRFVTGP
IATRLGVETLIATECEMIDGRYTGRSTDVPCFREGKVTRLNRWLDETGHNLEDSYFYSDS
MNDLSLLEQVANPIAVDPDPNLRAEAEKRGWPVISLRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory