SitesBLAST
Comparing Pf1N1B4_2373 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2373 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 99% coverage: 5:411/413 of query aligns to 4:359/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I8), G8 (= G9), G10 (= G11), V11 (= V12), I12 (= I13), V30 (≠ I31), E31 (= E32), K32 (≠ R34), E38 (= E40), A39 (≠ T41), S40 (= S42), A43 (≠ N45), G45 (= G47), L46 (≠ Q48), V171 (= V222), G200 (≠ A251), G201 (= G252), W203 (≠ R254), G298 (= G351), R300 (= R353), P301 (= P354), Y326 (≠ G378), R327 (≠ A379), N328 (≠ L380), G329 (= G381), I330 (≠ F382)
6j39A Crystal structure of cmis2 with inhibitor (see paper)
28% identity, 58% coverage: 166:403/413 of query aligns to 121:359/368 of 6j39A
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: R243 (vs. gap), Y252 (≠ I297), Y267 (≠ V312), R308 (= R353), R334 (≠ A379), I335 (≠ L380)
- binding flavin-adenine dinucleotide: A174 (≠ R213), A203 (= A251), W206 (≠ R254), I228 (≠ S274), Y252 (≠ I297), R308 (= R353), S333 (≠ G378), R334 (≠ A379), I335 (≠ L380), G336 (= G381), V337 (≠ F382), Q338 (≠ T383)
Sites not aligning to the query:
- binding (3R)-3-[(carboxymethyl)sulfanyl]nonanoic acid: 44, 46, 49
- binding flavin-adenine dinucleotide: 7, 9, 10, 11, 29, 30, 31, 32, 36, 37, 38, 40, 41, 42, 43, 44
6j38A Crystal structure of cmis2 (see paper)
28% identity, 58% coverage: 166:403/413 of query aligns to 121:359/368 of 6j38A
- binding flavin-adenine dinucleotide: A174 (≠ R213), A203 (= A251), W206 (≠ R254), G226 (= G272), G306 (= G351), R308 (= R353), S333 (≠ G378), R334 (≠ A379), I335 (≠ L380), G336 (= G381), V337 (≠ F382), Q338 (≠ T383)
Sites not aligning to the query:
- binding flavin-adenine dinucleotide: 7, 9, 11, 29, 30, 31, 36, 37, 38, 41, 42, 43, 44
4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 64% coverage: 140:405/413 of query aligns to 96:360/368 of 4yshB
- active site: I262 (≠ V308), L283 (≠ I329), G305 (= G351), N335 (≠ L380), L338 (≠ T383)
- binding flavin-adenine dinucleotide: V178 (= V222), S206 (≠ A251), W209 (≠ R254), R307 (= R353), H332 (= H377), R334 (≠ A379), N335 (≠ L380), G336 (= G381), I337 (≠ F382), L338 (≠ T383)
- binding glycine: G249 (≠ R295), Y251 (≠ I297), Y251 (≠ I297), A264 (= A310), R307 (= R353), R334 (≠ A379), R334 (≠ A379)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48
4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 64% coverage: 140:405/413 of query aligns to 96:360/370 of 4yshA
- active site: I262 (≠ V308), L283 (≠ I329), G305 (= G351), N335 (≠ L380), L338 (≠ T383)
- binding flavin-adenine dinucleotide: V178 (= V222), S206 (≠ A251), G207 (= G252), W209 (≠ R254), R307 (= R353), H332 (= H377), R334 (≠ A379), N335 (≠ L380), G336 (= G381), I337 (≠ F382)
Sites not aligning to the query:
- active site: 45, 48, 49, 52
- binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48
Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
24% identity, 64% coverage: 140:405/413 of query aligns to 97:362/377 of Q5L2C2
- V180 (= V222) binding
- R309 (= R353) binding
- 334:340 (vs. 377:383, 29% identical) binding
- R336 (≠ A379) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
Q9AGP8 Dimethylglycine oxidase; DMGO; EC 1.5.3.10 from Arthrobacter globiformis (see 2 papers)
26% identity, 57% coverage: 162:396/413 of query aligns to 115:376/830 of Q9AGP8
- V174 (= V222) binding
- H225 (≠ K271) Important for catalytic activity; mutation to Q: Reduces catalytic efficiency 3-fold and substrate affinity 30-fold.
- Y259 (= Y299) Important for catalytic activity; binding ; mutation to F: Reduces catalytic efficiency 225-fold and substrate affinity 25-fold.
- VWVT 360:363 (≠ LGFT 380:383) binding
Sites not aligning to the query:
- 14:15 binding
- 35:36 binding
- 45:48 binding
- 52 binding
- 539 binding
- 552 Important for catalytic activity; D→A: No effect on the activity.; D→N: Reduces activity 3-fold.
1pj6A Crystal structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folic acid (see paper)
26% identity, 57% coverage: 162:396/413 of query aligns to 113:374/828 of 1pj6A
- active site: H223 (≠ K271), Y257 (= Y299)
- binding flavin-adenine dinucleotide: V172 (= V222), A201 (= A251), G202 (= G252), W204 (≠ R254), H223 (≠ K271), Y257 (= Y299), G331 (= G351), I332 (≠ M352), V358 (≠ L380), W359 (≠ G381), V360 (≠ F382), T361 (= T383)
Sites not aligning to the query:
- active site: 550
- binding flavin-adenine dinucleotide: 9, 11, 12, 13, 33, 34, 42, 43, 44, 46, 48, 50
1pj7A Structure of dimethylglycine oxidase of arthrobacter globiformis in complex with folinic acid (see paper)
26% identity, 57% coverage: 162:396/413 of query aligns to 112:373/827 of 1pj7A
- active site: H222 (≠ K271), Y256 (= Y299)
- binding flavin-adenine dinucleotide: T170 (= T221), V171 (= V222), A200 (= A251), G201 (= G252), W203 (≠ R254), H222 (≠ K271), Y256 (= Y299), I331 (≠ M352), V357 (≠ L380), W358 (≠ G381), V359 (≠ F382), T360 (= T383)
Sites not aligning to the query:
- active site: 549
- binding flavin-adenine dinucleotide: 8, 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: 505, 536, 549, 551, 563, 629, 648, 655, 696
3gsiA Crystal structure of d552a dimethylglycine oxidase mutant of arthrobacter globiformis in complex with tetrahydrofolate (see paper)
26% identity, 57% coverage: 162:396/413 of query aligns to 112:373/827 of 3gsiA
- active site: H222 (≠ K271), Y256 (= Y299)
- binding flavin-adenine dinucleotide: T170 (= T221), V171 (= V222), A200 (= A251), G201 (= G252), W203 (≠ R254), H222 (≠ K271), Y256 (= Y299), G330 (= G351), I331 (≠ M352), F332 (≠ R353), V357 (≠ L380), W358 (≠ G381), V359 (≠ F382), T360 (= T383)
- binding magnesium ion: D254 (≠ I297)
Sites not aligning to the query:
- active site: 549
- binding flavin-adenine dinucleotide: 10, 11, 12, 32, 33, 41, 42, 43, 45, 47, 49
- binding magnesium ion: 409
- binding (6s)-5,6,7,8-tetrahydrofolate: 505, 536, 551, 563, 629, 648, 655, 696
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 75% coverage: 92:399/413 of query aligns to 46:349/364 of 3if9A
- active site: A47 (= A93), G48 (≠ F94), M49 (≠ L95)
- binding flavin-adenine dinucleotide: A46 (= A92), A47 (= A93), G48 (≠ F94), M49 (≠ L95), P173 (≠ T221), V174 (= V222), S202 (≠ A251), G203 (= G252), W205 (≠ R254), F209 (≠ L258), G300 (= G351), R302 (= R353), H327 (= H377), F328 (≠ G378), R329 (≠ A379), N330 (≠ L380), G331 (= G381), I332 (≠ F382)
- binding glycolic acid: Y246 (≠ I297), R302 (= R353), R329 (≠ A379)
Sites not aligning to the query:
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 70% coverage: 110:399/413 of query aligns to 62:349/369 of O31616
- V174 (= V222) binding
- H244 (≠ R295) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (= R353) binding
- 327:333 (vs. 377:383, 29% identical) binding
- R329 (≠ A379) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 70% coverage: 110:399/413 of query aligns to 62:349/364 of 1ng3A
- binding acetylamino-acetic acid: Y246 (≠ I297), R302 (= R353), R329 (≠ A379)
- binding flavin-adenine dinucleotide: V174 (= V222), S202 (≠ A251), G203 (= G252), W205 (≠ R254), F209 (≠ L258), G300 (= G351), R302 (= R353), H327 (= H377), R329 (≠ A379), N330 (≠ L380), G331 (= G381), I332 (≠ F382)
- binding phosphate ion: R89 (= R136), R254 (≠ Q305)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
S5FMM4 Glycine oxidase; GO; BliGO; EC 1.4.3.19 from Bacillus licheniformis (see paper)
25% identity, 65% coverage: 130:396/413 of query aligns to 83:346/369 of S5FMM4
- S202 (≠ A251) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
- I332 (≠ F382) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
- M342 (≠ L392) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.
Sites not aligning to the query:
- 51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
- 54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
- 81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
Query Sequence
>Pf1N1B4_2373 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2373
MAQRVCIIGGGVIGLTTAYALVRDGIDVMLIEARDSFASETSFANGGQLSYRYVAPLADA
GVPLQALGWMLRGDSPLKLRPRLDPAQWRWMAAFLAACRRSVNQRNGAHLLRLALLSQAT
LQGWRDEDRLDGFDWRRNGKLVTFREAASFEHACHSLADPRQQQVLSRTECAQLEPALAE
APFVGAIYTPDEEVADCHAFCLQLVARLKASGRCEFLPGRTVTGIRHGDGAVQAVEMGAQ
VLPVEQLVIAAGHRSPALALPGMNLPLYPLKGYSLTMPIRTEHRAPELSITDYNRKIVYA
RIGAQLRVAAMVDIVGFDPALDPKRLALIKRQAQETLPNAGDYDAAIEWAGMRPATPSGV
PLIGATAYRNLWLNLGHGALGFTLACGSARLLSELIARRTPSIEMQGLAPRVA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory