SitesBLAST
Comparing Pf1N1B4_24 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_24 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
81% identity, 97% coverage: 1:423/437 of query aligns to 25:442/488 of 8vc5A
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 2cv2A
- active site: K246 (= K230)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: E41 (= E19), Y187 (= Y171), R205 (= R189), A206 (≠ G190), E208 (= E192), W209 (= W193), L235 (= L219), L236 (= L220)
- binding : T43 (= T21), D44 (= D22), R47 (= R25), V145 (= V129), R163 (= R147), Y168 (≠ I152), E172 (≠ R156), V177 (= V161), K180 (= K164), S181 (≠ T165), Y187 (= Y171), E207 (= E191), E208 (= E192), W209 (= W193), V211 (≠ P195), R237 (= R221), K241 (= K225), L272 (≠ R256), M273 (= M257), G274 (= G258), E282 (= E266), S299 (= S283), P303 (= P287), V304 (≠ I288), K309 (= K293), W312 (= W296), R319 (= R303), P357 (≠ G333), R358 (= R334), R417 (≠ Q397), Q432 (≠ P412), R435 (≠ F415)
Sites not aligning to the query:
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: 5, 7, 9, 17, 21
- binding : 9, 442, 443, 444, 446, 447, 448
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 2cv1A
- active site: K246 (= K230)
- binding adenosine-5'-triphosphate: R47 (= R25), A206 (≠ G190), W209 (= W193), L235 (= L219), L236 (= L220)
- binding (4s)-4-amino-5-hydroxypentanoic acid: E41 (= E19), Y187 (= Y171), R205 (= R189), W209 (= W193)
- binding : E41 (= E19), T43 (= T21), D44 (= D22), R47 (= R25), V145 (= V129), R163 (= R147), V166 (= V150), E172 (≠ R156), V177 (= V161), K180 (= K164), S181 (≠ T165), Y187 (= Y171), E207 (= E191), E208 (= E192), W209 (= W193), V211 (≠ P195), R237 (= R221), K241 (= K225), K243 (= K227), M273 (= M257), G274 (= G258), S276 (= S260), E282 (= E266), S299 (= S283), P303 (= P287), V304 (≠ I288), K309 (= K293), W312 (= W296), R319 (= R303), P357 (≠ G333), R358 (= R334), R417 (≠ Q397), L427 (= L407), Q432 (≠ P412), R435 (≠ F415)
Sites not aligning to the query:
- binding adenosine-5'-triphosphate: 8, 9, 17, 18, 21
- binding (4s)-4-amino-5-hydroxypentanoic acid: 5, 7
- binding : 9, 442, 443, 444, 446, 447, 448
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 2cuzA
Sites not aligning to the query:
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 1n78A
- active site: K246 (= K230)
- binding glutamol-amp: E41 (= E19), Y187 (= Y171), N191 (= N175), R205 (= R189), A206 (≠ G190), E208 (= E192), W209 (= W193), L235 (= L219), L236 (= L220)
- binding : T43 (= T21), D44 (= D22), R47 (= R25), V145 (= V129), R163 (= R147), V166 (= V150), Y168 (≠ I152), E172 (≠ R156), V177 (= V161), K180 (= K164), S181 (≠ T165), Y187 (= Y171), E207 (= E191), E208 (= E192), W209 (= W193), L210 (= L194), V211 (≠ P195), R237 (= R221), K241 (= K225), M273 (= M257), G274 (= G258), E282 (= E266), R297 (= R281), P303 (= P287), V304 (≠ I288), K309 (= K293), W312 (= W296), R319 (= R303), P357 (≠ G333), R358 (= R334), R417 (≠ Q397), L427 (= L407), Q432 (≠ P412), R435 (≠ F415)
Sites not aligning to the query:
- binding glutamol-amp: 5, 7, 8, 9, 17, 18, 21
- binding : 9, 442, 443, 444, 446, 447, 448
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 1j09A
- active site: K246 (= K230)
- binding adenosine-5'-triphosphate: E208 (= E192), L235 (= L219), L236 (= L220), K243 (= K227), I244 (≠ L228), S245 (= S229), K246 (= K230), R247 (= R231)
- binding glutamic acid: E41 (= E19), Y187 (= Y171), N191 (= N175), R205 (= R189), W209 (= W193)
Sites not aligning to the query:
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of P27000
- R358 (= R334) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
45% identity, 96% coverage: 1:420/437 of query aligns to 23:440/468 of 1g59A
- binding : D44 (= D22), R45 (≠ Q23), A46 (≠ L24), R47 (= R25), P109 (≠ A87), V145 (= V129), R163 (= R147), V166 (= V150), E172 (≠ R156), V177 (= V161), K180 (= K164), S181 (≠ T165), D182 (= D166), E207 (= E191), E208 (= E192), R237 (= R221), K241 (= K225), T242 (≠ S226), K243 (= K227), M273 (= M257), G274 (= G258), E282 (= E266), S299 (= S283), L300 (= L284), P303 (= P287), V304 (≠ I288), K309 (= K293), W312 (= W296), R319 (= R303), P357 (≠ G333), R358 (= R334), R417 (≠ Q397), K426 (= K406), L427 (= L407), Q432 (≠ P412), R435 (≠ F415)
Sites not aligning to the query:
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
36% identity, 96% coverage: 1:421/437 of query aligns to 23:454/485 of 4griB
Sites not aligning to the query:
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
37% identity, 96% coverage: 1:418/437 of query aligns to 24:470/502 of 6brlA
Sites not aligning to the query:
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 96% coverage: 1:420/437 of query aligns to 24:434/471 of P04805