SitesBLAST

P54582 Glycine betaine transporter BetP; Glycine betaine permease from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see 6 papers)
35% identity, 75% coverage: 56:557/668 of query aligns to 98:591/595 of P54582

query
sites
P54582

S

N

F

L

G

G

W
|
W

Y

A

Y

F

M

I

V

L

V

F

I

G

A

T

A

V

Y

F

L

V

V

F

F

F

V

I

V

V

G

V

L

I

A

A

F

A

S

S

S

K

Y

F

G

G

K

T

L

I

K

R

L

L

G

G

S

R

K

I

D

D

D

E

T

A

P

P

D
x
E

F

F

S

R

Y

T

G

V

A

S

W

W

A

I

G

S

M

M

L

M

F

F

S

A

G
|
G

I
x
M

G
|
G

I
|
I

S
x
G

L

L

L

M

Y
x
F

F

Y

G

G

A

T

S

T

E

E

P

P

L

L

D

T

H

F

Y

Y

F

R

N

N

P

G

P

V

E

P

G

G

V

H

A

D

G

E

S

H

N

N

L

V

A

G

A

V

R

-

Q

-

A

A

V

M

Q

S

L

T

T

T

F

M

L

F

H

H

W
|
W

G

T

L

L

H

H

G

P

W
|
W

A

A

I

I

Y
|
Y

A

A

L

I

V

V

G

G

L

L

A

A

V

I

A

A

Y

Y

F

S

A

T

Y

F

R
|
R

H

V

N

G

Q

R

P

K

L

Q

A

L

L

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

V

I

G

G

E

E

R

K

W

G

V

A

K

E

G

G

A

W

A

L

G

G

H

K

A

L

V

I

D

D

G

I

F

L

G

A

M

I

F

I

V

A

T

T

L

V

L

F

G

G

L

T

V

A

T

C

N
x
S

L

L

G

G

I

L

G

G

S

A

L

L

Q

Q

V

I

S

G

S

A

G

G

L

L

E

S

N

A

L

A

F

N

G

I

M

I

E

E

H

D

S

P

N

S

T

D

N

W

L

T

L

I

I

V

V

G

I

I

I

V

V

S

M

V

S

L

T

T

V

L

A

A

T

F

I

I

-

F

A

S

A

A

V

I

S

S

G

G

V

V

E

G

N

K

G
|
G

I

I

R

Q

R

Y

L

L

S

S

N

N

L

A

N
|
N

I

M

V

V

L

L

F

A

S

A

G

L

L

L

L

A

I

I

F

F

V

V

L

F

L

V

F

V

G

G

P

P

T

T

L

V

H

S

L

I

L

L

N

N

G

L

F

L

V

P

Q

G

N

S

I

I

G

G

D

N

Y

Y

L

L

N

S

G

-

V

-

L

-

K

-

T

-

F

-

D

N

L

F

Y

F

V

Q

Y

M

E

A

G

G

D

R

S
x
T

E

A

K

M

S

S

-

A

-

D

-

G

-

T

-

A

D

G

R

E

W
|
W

L

L

G

G

L

S

W
|
W

T

T

L

I

F
|
F

Y

Y

W
|
W

A

A

W
|
W

I
|
I

S
|
S

W
|
W

A

S

P

P

F
|
F

V

V

G

G

M

M

F
|
F

I

L

A

A

R
|
R

I

I

S

S

R

R

G

G

R
|
R

T

S

V

I

R

R

E

E

L

F

V

I

A

L

G

G

V

V

L

L

I

V

P

P

L

A

G

G

F

V

T

S

L

T

A

V

W

W

L

F

S

S

I

I

F

F

G

G

N

G

S

T

A

A

L

I

D

V

L

F

V

E

M

Q

N

N

H

G

G

E

A

S

V

I

E

W

L

G

G

D

K

G

T

A

A

A

L

E

E

E

Q

Q

P

-

S

-

M

-

A

-

I

L

Y

F

Q

G

L

L

E

H

H

A

Y

L

P

P

A

G

S

G

K

Q

I

I

V

M

I

G

G

I

V

I

S

A

I

M

F

I

V

L

G

L

F

G

V

T

L

F

F

F

L

I

T

T

P

S

A

A

D

D

S

S

G

A

A

S

V

T

M

V

M

M

A

G

N

T

L

M

S

S

C

Q

K

H

G

G

G

-

N

-

V

-

D

Q

E

L

D

E

A

A

P

N

H

K

W

W

L

V

R

T

I

A

F

A

W

W

S

G

A

V

V

A

I

T

T

A

L

A

V

I

T

G

I

L

G

T

L

L

F

L

A

S

G

G

N

G

F

D

E

N

A

A

-

L

-

S

-

N

M

L

Q

Q

T

N

M

V

V

T

V

I

L

V

A

A

G

A

L

T

P

P

F

F

S

L

V

F

V

V

L

V

V

I

F

G

F

L

M

M

F

F

G

A

L

L

H

V

K

K

A

D

M

L

R

S

Q

N

D

D

V

V

-

I

Q

Y

I

L

E

E

-

Y

Q

R

E

E

Q

Q

A

Q

E

R

L

F

A

N

A

A

R

R

G

L

R

A

R

R

G

-

F

-

S

-

E

E

R

R

L

R

T

V

Q

H

L

N

D

E

L

H

Q

R

P

K

N

R

Q

E

S

L

I

A

V

A

Q

K

R

R

F

R

M

R

D

E

K

R

R

K

V

A

S

S

P

G

A

A

W101 (= W59) mutation to A: Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-351.
E135 (≠ D93) mutation to A: Strongly decreased betaine transport.
G149 (= G107) mutation to A: Decreases betaine transport. No effect on activation by increased osmolality.
M150 (≠ I108) mutation to F: No effect on activation by increased osmolality; when associated with A-152.
G151 (= G109) mutation to A: Nearly abolishes betaine transport.
I152 (= I110) mutation to A: No effect on activation by increased osmolality; when associated with F-150.
IG 152:153 (≠ IS 110:111) binding glycine betaine
G153 (≠ S111) mutation to A: Decreases betaine transport and alters activation at higher osmolality.; mutation to D: Changes substrate specificity, giving rise to proton-coupled choline transport. Decreases sodium-dependent betaine transport.
F156 (≠ Y114) mutation to A: Decreases betaine transport, but has no major effect on affinity for glycine betaine.
W189 (= W149) mutation to C: Mildly decreased betaine transport.
W194 (= W154) mutation to L: Strongly decreased betaine transport.
Y197 (= Y157) mutation to L: Nearly abolishes betaine transport.
R210 (= R170) mutation to A: Nearly abolishes betaine transport.
S253 (≠ N213) binding glycine betaine
G301 (= G260) mutation to L: Strongly decreased betaine transport.
N309 (= N268) mutation to A: Decreases affinity for sodium ions.
T351 (≠ S317) mutation to A: Mainly trimeric, but shows reduced activity at high osmolalities. Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-101.
W362 (= W323) mutation to C: Strongly decreased betaine transport.
W366 (= W327) mutation to C: No effect on betaine transport.
F369 (= F330) mutation to G: Decreases affinity for glycine betaine. Decreases betaine transport.
W371 (= W332) mutation to L: No effect on betaine transport.
W373 (= W334) mutation to A: Strongly decreases affinity for glycine betaine and betaine transport.
WWISW 373:377 (= WWISW 334:338) binding glycine betaine
W374 (= W335) mutation to A: Strongly decreases betaine transport, but has no major effect on affinity for glycine betaine.; mutation to L: No effect on betaine transport.
W377 (= W338) mutation to A: Abolishes betaine transport.; mutation to L: Nearly abolishes betaine transport.
F380 (= F341) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
F384 (= F345) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
R387 (= R348) mutation to A: Mildly decreased betaine transport.
R392 (= R353) mutation to K: Moderately decreased betaine transport.

4llhA Substrate bound outward-open state of the symporter betp (see paper)
35% identity, 75% coverage: 56:553/668 of query aligns to 42:523/524 of 4llhA

query
sites
4llhA

S

N

F

L

G

G

W

W

Y

A

Y

F

M

I

V

L

V

F

I

G

A

T

A

V

Y

F

L

V

V

F

F

F

V

I

V

V

G

V

L

I

A

A

F

A

S

S

S

K

Y

F

G

G

K

T

L

I

K

R

L

L

G

G

S

R

K

I

D

D

D

E

T

A

P

P

D

E

F

F

S

R

Y

T

G

V

A

S

W

W

A

I

G

S

M

M

L

M

F

F

S
x
A

S

A

G

G

I
x
M

G
|
G

I

I

S
x
D

L

L

L

M

Y

F

F

Y

G

G

A

T

S

T

E

E

P

P

L

L

D

T

H

F

Y

Y

F

R

N

N

P

G

P

V

E

P

G

G

V

H

A

D

G

E

S

H

N

N

L

V

A

G

A

V

R

-

Q

-

A

A

V

M

Q

S

L

T

T

T

F

M

L

F

H

H

W

W

G

T

L

L

H

H

G

P

W

W

A

A

I

I

Y

Y

A

A

L

I

V

V

G

G

L

L

A

A

V

I

A

A

Y

Y

F

S

A

T

Y

F

R

R

H

V

N

G

Q

R

P

K

L

Q

A

L

L

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

V

I

G

G

E

E

R

K

W

G

V

A

K

E

G

G

A

W

A

L

G

G

H

K

A

L

V

I

D

D

G

I

F

L

G

A

M

I

F

I

V

A

T

T

L

V

L

F

G

G

L

T

V

A

T

C

N

S

L

L

G

G

I

L

G

G

S

A

L

L

Q

Q

V

I

S

G

S

A

G

G

L

L

E

S

N

A

L

-

F

-

G

A

M

E

E

D

H

P

S

S

N

D

T

W

N

T

L

I

L

V

I

G

V

I

I

V

I

S

V

V

M

L

S

T

T

L

V

A

A

F

T

I

I

F

A

S

A

A

V

I

S

S

G

G

V

V

E

G

N

K

G

G

I

I

R

Q

R

Y

L

L

S

S

N

N

L

A

N

N

I

M

V

V

L

L

F

A

S

A

G

L

L

L

L

A

I

I

F

F

V

V

L

F

L

V

F

V

G

G

P

P

T

T

L

V

H

S

L

I

L

L

N

N

G

L

F

L

V

P

Q

G

N

S

I

I

G

G

D

N

Y

Y

L

L

N

S

G

-

V

-

L

-

K

-

T

-

F

-

D

N

L

F

Y

F

V

Q

Y

M

E

A

G

G

D

R

S

T

E

A

K

M

S

S

-

A

-

D

-

G

-

T

-

A

D

G

R

E

W

W

L

L

G

G

L

S

W

W

T

T

L

I

F

F

Y

Y

W

W

A

A

W
|
W

I

I

S

S

W
|
W

A

S

P

P

F

F

V

V

G

G

M

M

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

S

V

I

R

R

E

E

L

F

V

I

A

L

G

G

V

V

L

L

I

V

P

P

L

A

G

G

F

V

T

S

L

T

A

V

W

W

L

F

S

S

I

I

F

F

G

G

N

G

S

T

A

A

L

I

D

V

L

F

V

E

M

Q

N

N

H

G

G

E

A

S

V

I

E

W

L

G

G

D

K

G

T

A

A

A

L

E

E

E

Q

Q

P

-

S

-

M

-

A

-

I

L

Y

F

Q

G

L

L

E

H

H

A

Y

L

P

P

A

G

S

G

K

Q

I

I

V

M

I

G

G

I

V

I

S

A

I

M

F

I

V

L

G

L

F

G

V

T

L
x
F

F

F

L

I

T
|
T

P
x
S

A

A

D

D

S

S

G

A

A

S

V

T

M

V

M

M

A

G

N

T

L

M

S

S

C

Q

K

H

G

G

G

-

N

-

V

-

D

Q

E

L

D

E

A

A

P

N

H

K

W

W

L

V

R

T

I

A

F

A

W

W

S

G

A

V

V

A

I

T

T

A

L

A

V

I

T

G

I

L

G

T

L

L

F

L

A

S

G

G

N

G

F

D

E

N

A

A

-

L

-

S

-

N

M

L

Q

Q

T

N

M

V

V

T

V

I

L

V

A

A

G

A

L

T

P

P

F

F

S

L

V

F

V

V

L

V

V

I

F

G

F

L

M

M

F

F

G

A

L

L

H

V

K

K

A

D

M

L

R

S

Q

N

D

D

V

V

Q

-

I

-

E

-

Q

-

E

-

Q

-

A

I

E

Y

L

L

A

E

A

Y

R
|
R

G

E

R

Q

G
x
R

F

F

S

N

E

A

R

R

L

L

T

A

Q

R

L

E

D

R

L

R

Q

V

P

H

N

N

Q

E

S

H

I

R

V

K

Q

R

R

E

F

L

M

A

D

A

K

K

R

R

binding 2-(trimethyl-lambda~5~-arsanyl)ethanol: M94 (≠ I108), G95 (= G109), D97 (≠ S111), W314 (= W334), W315 (= W335), W318 (= W338)
binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: R495 (= R525), R499 (≠ G529)
binding sodium ion: A91 (≠ S105), M94 (≠ I108), G95 (= G109), F405 (≠ L429), T408 (= T432), S409 (≠ P433)

3p03C Crystal structure of betp-g153d with choline bound (see paper)
36% identity, 71% coverage: 56:528/668 of query aligns to 42:505/508 of 3p03C

query
sites
3p03C

S

N

F

L

G

G

W

W

Y

A

Y

F

M

I

V

L

V

F

I

G

A

T

A

V

Y

F

L

V

V

F

F

F

V

I

V

V

G

V

L

I

A

A

F

A

S

S

S

K

Y

F

G

G

K

T

L

I

K

R

L

L

G

G

S

R

K

I

D

D

D

E

T

A

P

P

D

E

F

F

S

R

Y

T

G

V

A

S

W

W

A

I

G

S

M

M

L

M

F

F

S

A

S
x
A

G

G

I
x
M

G

G

I

I

S
x
D

L

L

L

M

Y

F

F

Y

G

G

A

T

S

T

E

E

P

P

L

L

D

T

H

F

Y

Y

F

R

N

N

P

G

P

V

E

P

G

G

V

H

A

D

G

E

S

H

N

N

L

V

A

G

A

V

R

-

Q

-

A

A

V

M

Q

S

L

T

T

T

F

M

L

F

H

H

W

W

G

T

L

L

H

H

G

P

W

W

A

A

I

I

Y

Y

A

A

L

I

V

V

G

G

L

L

A

A

V

I

A

A

Y

Y

F

S

A

T

Y

F

R

R

H

V

N

G

Q

R

P

K

L

Q

A

L

L

L

R

S

S

S

A

A

L

F

Y

V

P

P

L

L

V

I

G

G

E

E

R

K

W

G

V

A

K

E

G

G

A

W

A

L

G

G

H

K

A

L

V

I

D

D

G

I

F

L

G

A

M

I

F

I

V

A

T

T

L

V

L

F

G

G

L

T

V

A

T

C

N

S

L

L

G

G

I

L

G

G

S

A

L

L

Q

Q

V

I

S

G

S

A

G

G

L

L

E

S

N

-

L

-

F

-

G

A

M

A

E

N

H

I

S

I

N

D

T

W

N

T

L

I

L

V

I

G

V

I

I

V

I

S

V

V

M

L

S

T

T

L

V

A

A

F

T

I

I

F

A

S

A

A

V

I

S

S

G

G

V

V

E

G

N

K

G

G

I

I

R

Q

R

Y

L

L

S

S

N

N

L

A

N

N

I

M

V

V

L

L

F

A

S

A

G

L

L

L

L

A

I

I

F

F

V

V

L

F

L

V

F

V

G

G

P

P

T

T

L

V

H

S

L

I

L

L

N

N

G

L

F

L

V

P

Q

G

N

S

I

I

G

G

D

N

Y

Y

L

L

N

S

G

-

V

-

L

-

K

-

T

-

F

-

D

N

L

F

Y

F

V

Q

Y

M

E

A

G

G

D

R

S

T

E

A

K

M

S

S

-

A

-

D

-

G

-

T

-

A

D

G

R

E

W

W

L

L

G

G

L

S

W

W

T

T

L

I

F

F

Y

Y

W

W

A

A

W

W

I

I

S

S

W

W

A

S

P

P

F
|
F

V

V

G

G

M

M

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

S

V

I

R

R

E

E

L

F

V

I

A

L

G

G

V

V

L

L

I

V

P

P

L

A

G

G

F

V

T

S

L

T

A

V

W

W

L

F

S

S

I

I

F

F

G

G

N

G

S

T

A

A

L

I

D

V

L

F

V

E

M

Q

N

N

H

G

G

E

A

S

V

I

E

W

L

G

G

D

K

G

T

A

A

A

L

E

E

E

Q

Q

P

-

S

-

M

-

A

-

I

L

Y

F

Q

G

L

L

E

H

H

A

Y

L

P

P

A

G

S

G

K

Q

I

I

V

M

I

G

G

I

V

I

S

A

I

M

F

I

V

L

G

L

F

G

V

T

L

F

F

F

L

I

T

T

P

S

A

A

D

D

S

S

G

A

A

S

V

T

M

V

M

M

A

G

N

T

L

M

S

S

C

Q

K

H

G

G

G

-

N

-

V

-

D

Q

E

L

D

E

A

A

P

N

H

K

W

W

L

V

R

T

I

A

F

A

W

W

S

G

A

V

V

A

I

T

T

A

L

A

V

I

T

G

I

L

G

T

L

L

F

L

A

S

G

G

N

G

F

D

E

N

A

A

-

L

-

S

-

N

M

L

Q

Q

T

N

M

V

V

T

V

I

L

V

A

A

G

A

L

T

P

P

F

F

S

L

V

F

V

V

L

V

V

I

F

G

F

L

M

M

F

F

G

A

L

L

H

V

K

K

A

D

M

L

R

S

Q

N

D

D

V

V

-

I

Q

Y

I

L

E

E

-

Y

Q

R

E

E

Q

Q

A

Q

E

R

L

F

A

N

A

A

R

R

G

L

R

A

R

R

binding choline ion: A92 (≠ S106), M94 (≠ I108), D97 (≠ S111), F320 (= F341)

2wswA Crystal structure of carnitine transporter from proteus mirabilis (see paper)
28% identity, 66% coverage: 56:493/668 of query aligns to 59:487/508 of 2wswA

query
sites
2wswA

S

A

F

F

G

E

W

W

Y

Y

Y

M

M

V

V

I

V

M

I

F

A

G

Y

W

L
x
F

V

-

F

-

V

-

G

W

L

L

A

V

F
|
F

S

G

S
x
R

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

D

D

E

T

K

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E

E

P

I

L

Y

D

Y

H

Y

Y

I

F

S

N

S

P

P

E

F

G

G

V

M

A

E

G

G

S

Y

N

S

L

A

A

P

A

A

R

K

Q

E

-

I

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W

W

G

G

L

P

H

L

G

P

W

W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

V

K

N

G

G

A

L

A

F

G

G

H

T

A

V

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

L

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

I

E

Q

N

Y

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

S

V

C

M

W

S

I

T

L

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

F

G

G

V

L

E

Q

N

K

G

G

I

V

R

K

R

I

L

A

S

S

N

D

L

V

N

R

I

T

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

G

P

G

T

A

L

S

H

F

L

I

L

V

N

N

G

Y

F

F

V

T

Q

D

N

S

I

V

G

G

D

T

Y

-

L

-

N

-

G

-

V

-

V

L

L

L

K

M

T

Y

F

M

D

P

L

R

Y

M

V

L

Y

F

E

Y

G

T

D

D

S

P

E

I

K

G

S

K

D

G

R

G

W

F

L

P

G

Q

L

A

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

A

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

L

G

G

V

M

L

V

L

S

-

G

I

L

P

T

L

A

G

G

F

T

T

W

L

L

A

I

W

W

L

-

S

T

I

I

F

L

G

G

N

G

S

N

A

T

L

L

D

Q

L

L

V

I

M

-

N

-

H

-

G

-

A

-

V

-

E

-

L

-

G

-

K

-

T

-

A

-

L

-

E

D

Q

Q

P

N

S

I

M

L

A

N

I

I

Y

P

Q

Q

L

L

L

I

E

D

H

Q

Y

Y

-

G

-

V

-

P

-

R

-

A

-

I

-

E

-

T

-

W

-

A

-

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

I

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

S

G

M

N

K

V

E

D

G

E

A

D

D

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

V

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

I

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q

Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: F72 (≠ L69), F76 (= F77), R78 (≠ S79)

P31553 L-carnitine/gamma-butyrobetaine antiporter from Escherichia coli (strain K12) (see 3 papers)
26% identity, 76% coverage: 7:512/668 of query aligns to 1:501/504 of P31553

query
sites
P31553

I

M

K

K

T

N

P

E

P

K

E

R

K

K

V

T

T

G

V

I

N

E

G

P

W

K

V

V

F

F

Y

F

T

P

S

P

T

L

A

I

L

I

I

V

L

G

L

I

L

L

T

C

A

W

I

L

L

T

I

V

I

R

A

D

P

L

Q

D

E

A

A

A

G

N

R

V

M

V

L

I

G

N

I

A

A

V

Q

F

A

S

W

Y

L

V

S

T

R

N

S

V

F

W

G

G

W

W

-

A

-

F

-

E

Y

W

Y

Y

M

M

V

V

I

M

A

-

Y

-

L

L

V

F

F

G

V

W

V

F

G

W

L

L

A

V

F

F

S

G

S

P

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

N

D

E

T

P

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E

E

P

I

L

Y

D
x
Y

H

Y

Y

I

F

S

N

T

P

P

E

F

G

G

V

L

A

E

G

P

S

N

N

S

L

T

A

G

A

A

R

K

Q

E

-

L

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W
|
W

G

G

L

P

H

L

G

P

W

W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

A

K

K

G

G

A

L

A

F

G

G

H

T

A

I

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

F

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

M

E

Q

N

W

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

T

V

C

M

W

S

I

T

I

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

C

G

G

V

L

E

Q

N

K

G

G

I

V

R

R

R

I

L

A

S

S

N

D

L

V

N

R

I

S

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

S

P

G

T

A

L

S

H

F

L

I

L

M

N

N

G

Y

F

F

V

T

Q
x
D

N

S

I

V

G

G

D

-

Y

-

L

-

N

-

G

-

V

M

V

L

L

L

K
x
M

T

Y

F

L

D

P

L
x
R

Y

M

V

L

Y

F

E

Y

G
x
T

D

D

S

P

E

I

K

A

S

K

D

G

R

G

W

F

L

P

G

Q

L
x
G

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

A
|
A

P
x
I

F
x
Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

F

G

G

V

M

L

V

L

L

I

G

P

L

L

T

G

A

F

S

T

T

L

W

A

I

W

L

L

W

S

T

I

V

F

L

G

G

N

S

S

N

A

T

L

L

D

-

L

L

V

L

M

I

N

D

H

K

G

N

A

I

V

I

E

N

L

I

G

-

K

P

T

N

A

L

L

I

E

E

Q

Q

-

Y

-

G

P

V

S

A

M

R

A

A

I

I

Y

I

Q

E

L

T

L

W

E

A

H

A

Y

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

V

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

E

G

V

N

R

V

D

D

G

E

E

D

E

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

I

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

V

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q

Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

F

L

S

F

V

F

V

V

L

N

V

I

F

M

F

V

M

T

F

L

G

S

L

F

H

I

K

K

A

D

M

A

R

K

Q

Q

D

N

Y114 (≠ D122) binding 4-(trimethylamino)butanoate; mutation to L: Small decrease in transport activity.
W142 (= W149) binding (R)-carnitine
D288 (≠ Q294) mutation to A: Retains 70% of transport activity. Forms mostly monomers.; mutation to R: Abolishes transport activity. Forms mostly monomers.; mutation to W: Retains 4% of transport activity. Forms mostly monomers.
M295 (≠ K306) mutation to E: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers.
R299 (≠ L310) mutation to A: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers. Shows a high tendency to aggregate.
T304 (≠ G315) mutation to A: Does not affect transport activity. Forms mostly monomers. Shows a high tendency to aggregate.
GW 315:316 (≠ LW 326:327) binding 4-(trimethylamino)butanoate
W316 (= W327) mutation to L: Decrease in transport activity.
W323 (= W334) binding 4-(trimethylamino)butanoate; mutation to L: Abolishes transport activity.
WW 323:324 (= WW 334:335) binding (R)-carnitine
W324 (= W335) mutation to L: Abolishes transport activity.
Y327 (≠ W338) mutation to L: Strong decrease in transport activity.
YAIQ 327:330 (≠ WAPF 338:341) binding (R)-carnitine
Q330 (≠ F341) mutation to L: Decrease in transport activity.
M331 (≠ V342) binding 4-(trimethylamino)butanoate

4m8jA Crystal structure of cait r262e bound to gamma-butyrobetaine (see paper)
28% identity, 66% coverage: 56:493/668 of query aligns to 46:474/495 of 4m8jA

query
sites
4m8jA

S

A

F

F

G

E

W

W

Y

Y

Y

M

M

V

V

I

V

M

I

F

A

G

Y

W

L

F

V

-

F

-

V

-

G

W

L

L

A

V

F

F

S

G

S

R

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

D

D

E

T

K

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E

E

P

I

L

Y

D

Y

H

Y

Y

I

F

S

N

S

P

P

E

F

G

G

V

M

A

E

G

G

S

Y

N

S

L

A

A

P

A

A

R

K

Q

E

-

I

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W

W

G

G

L

P

H

L

G

P

W
|
W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

V

K

N

G

G

A

L

A

F

G

G

H

T

A

V

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

L

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

I

E

Q

N

Y

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

S

V

C

M

W

S

I

T

L

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

F

G

G

V

L

E

Q

N

K

G

G

I

V

R

K

R

I

L

A

S

S

N

D

L

V

N

E

I

T

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

G

P

G

T

A

L

S

H

F

L

I

L

V

N

N

G

Y

F

F

V

T

Q

D

N

S

I

V

G

G

D

T

Y

-

L

-

N

-

G

-

V

-

V

L

L

L

K

M

T

Y

F

M

D

P

L

R

Y

M

V

L

Y

F

E

Y

G

T

D

D

S

P

E

I

K

G

S

K

D

G

R

G

W

F

L

P

G

Q

L

A

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W

Y

A

A

P

I

F

Q

V

M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

L

G

G

V

M

L

V

L

S

-

G

I

L

P

T

L

A

G

G

F

T

T

W

L

L

A

I

W

W

L

-

S

T

I

I

F

L

G

G

N

G

S

N

A

T

L

L

D

Q

L

L

V

I

M

-

N

-

H

-

G

-

A

-

V

-

E

-

L

-

G

-

K

-

T

-

A

-

L

-

E

D

Q

Q

P

N

S

I

M

L

A

N

I

I

Y

P

Q

Q

L

L

L

I

E

D

H

Q

Y

Y

-

G

-

V

-

P

-

R

-

A

-

I

-

E

-

T

-

W

-

A

-

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

I

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

S

G

M

N

K

V

E

D

G

E

A

D

D

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

V

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

I

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q

Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W139 (= W154), W315 (= W334), W316 (= W335)

3hfxA Crystal structure of carnitine transporter (see paper)
27% identity, 68% coverage: 56:512/668 of query aligns to 43:490/493 of 3hfxA

query
sites
3hfxA

S

A

F

F

G

E

W

W

Y

Y

Y

M

M

V

V

V

V

M

I

L

A

F

A

G

Y

W

L

F

V

-

F

-

V

-

G

W

L

L

A

V

F

F

S

G

S

P

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

N

D

E

T

P

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E

E

P

I

L

Y

D
x
Y

H

Y

Y

I

F

S

N

T

P

P

E

F

G

G

V

L

A

E

G

P

S

N

N

S

L

T

A

G

A

A

R

K

Q

E

-

L

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W
|
W

G

G

L

P

H

L

G

P

W

W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

A

K

K

G

G

A

L

A

F

G

G

H

T

A

I

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

F

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

M

E

Q

N

W

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

T

V

C

M

W

S

I

T

I

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

C

G

G

V

L

E

Q

N

K

G

G

I

V

R

R

R

I

L

A

S

S

N

D

L

V

N

R

I

S

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

S

P

G

T

A

L

S

H

F

L

I

L

M

N

N

G

Y

F

F

V

T

Q

D

N

S

I

V

G

G

D

-

Y

-

L

-

N

-

G

-

V

M

V

L

L

L

K

M

T

Y

F

L

D

P

L

R

Y

M

V

L

Y

F

E

Y

G

T

D

D

S

P

E

I

K

A

S

K

D

G

R

G

W

F

L

P

G

Q

L

G

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

A

A

P

I

F
x
Q

V

M

G

S

M

I

F
|
F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

F

G

G

V

M

L

V

L

L

I

G

P

L

L

T

G

A

F

S

T

T

L

W

A

I

W

L

L

W

S

T

I

V

F

L

G

G

N

S

S

N

A

T

L

L

D

-

L

L

V

L

M

I

N

D

H

K

G

N

A

I

V

I

E

N

L

I

G

-

K

P

T

N

A

L

L

I

E

E

Q

Q

-

Y

-

G

P

V

S

A

M

R

A

A

I

I

Y

I

Q

E

L

T

L

W

E

A

H

A

Y

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

V

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

E

G

V

N

R

V

D

D

G

E

E

D

E

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

I

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

V

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q

Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

F

L

S

F

V

F

V

V

L

N

V

I

F

M

F

V

M

T

F

L

G

S

L

F

H

I

K

K

A

D

M

A

R

K

Q

Q

D

N

binding carnitine: Y103 (≠ D122), W131 (= W149), W305 (= W327), W312 (= W334), W313 (= W335), Y316 (≠ W338), Y316 (≠ W338), Q319 (≠ F341), F323 (= F345)

2wsxA Crystal structure of carnitine transporter from escherichia coli (see paper)
27% identity, 68% coverage: 56:512/668 of query aligns to 47:494/496 of 2wsxA

query
sites
2wsxA

S

A

F

F

G

E

W

W

Y

Y

Y

M

M

V

V

V

V

M

I

L

A

F

A

G

Y

W

L

F

V

-

F

-

V

-

G

W

L

L

A

V

F

F

S

G

S

P

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

E

T

P

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E

E

P

I

L

Y

D

Y

H

Y

Y

I

F

S

N

T

P

P

E

F

G

G

V

L

A

E

G

P

S

N

N

S

L

T

A

G

A

A

R

K

Q

E

-

L

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W
|
W

G

G

L

P

H

L

G

P

W

W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

V

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

A

K

K

G

G

A

L

A

F

G

G

H

T

A

I

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

F

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

M

E

Q

N

W

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

T

V

C

M

W

S

I

T

I

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

C

G

G

V

L

E

Q

N

K

G

A

I

V

R

R

R

I

L

A

S

S

N

D

L

V

N

R

I

S

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

S

P

G

T

A

L

S

H

F

L

I

L

M

N

N

G

Y

F

F

V

T

Q

D

N

S

I

V

G

G

D

-

Y

-

L

-

N

-

G

-

V

M

V

L

L

L

K

M

T

Y

F

L

D

P

L

R

Y

M

V

L

Y

F

E

Y

G

T

D

D

S

P

E

I

K

A

S

K

D

G

R
x
G

W

F

L

P

G

Q

L
x
G

W

W

T

T

L

V

F

F

Y

Y

W

W

A

A

W
|
W

W

W

I

V

S

I

W
x
Y

A

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

R

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

F

G

G

V

M

L

V

L

L

I

G

P

L

L

T

G

A

F

S

T

T

L

W

A

I

W

L

L

W

S

T

I

V

F

L

G

G

N

S

S

N

A

T

L

L

D

L

L

L

V

I

M

-

N

-

H

-

G

-

A

-

V

-

E

-

L

-

G

D

K

K

T

N

A

I

L

L

E

N

Q

I

P

P

S

N

M

L

-

I

-

E

-

Q

-

Y

-

G

-

V

-

A

-

R

A

A

I

I

Y

I

Q

E

L

T

L

W

E

A

H

A

Y

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

V

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

E

G

V

N

R

V

D

D

G

E

E

D

D

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

I

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

V

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q
|
Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

F

L

S

F

V

F

V

V

L

N

V

I

F

M

F

V

M

T

F

L

G

S

L

F

H

I

K

K

A

D

M

A

R

K

Q

Q

D

N

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W135 (= W149), G304 (≠ R322), G308 (≠ L326), W316 (= W334), Y320 (≠ W338), M324 (≠ V342), Q466 (= Q484)

B4EY22 L-carnitine/gamma-butyrobetaine antiporter from Proteus mirabilis (strain HI4320) (see 2 papers)
28% identity, 66% coverage: 56:493/668 of query aligns to 54:482/514 of B4EY22

query
sites
B4EY22

S

A

F

F

G

E

W

W

Y

Y

Y

M

M

V

V

I

V

M

I

F

A

G

Y

W

L

F

V

-

F

-

V

-

G

W

L

L

A

V

F

F

S

G

S

R

Y

Y

G

A

K

K

L

K

K

R

L

L

G

G

S

-

K

-

D

D

D

E

T

K

P

P

D

E

F

F

S

S

Y

T

G

A

A

S

W

W

A

I

G

F

M

M

L

M

F

F

S

A

S

S

G

C

I

T

G

S

I

A

S

A

L

V

L

L

Y

F

F

W

G

G

A

S

S

I

E
|
E

P

I

L

Y

D

Y

H

Y

Y

I

F

S

N

S

P

P

E

F

G

G

V

M

A

E

G

G

S

Y

N

S

L

A

A

P

A

A

R

K

Q

E

-

I

A

G

V

L

Q

A

L

Y

T

S

F

L

L

F

H

H

W

W

G

G

L

P

H

L

G

P

W

W

A

A

I

T

Y

Y

A

S

L

F

V

L

G

S

L

V

A

A

V

F

A

A

Y

Y

F

F

A

F

Y

F

-

V

R

R

H

K

N

M

Q

E

P

V

L

I

A

R

L

P

R

S

S

S

A

T

L

L

Y

T

P

P

L

L

V

V

G

G

E

E

R

K

W

H

V

V

K

N

G

G

A

L

A

F

G

G

H

T

A

V

V

V

D

D

G

N

F

F

G

Y

M

L

F

V

V

A

T

L

L

I

L

L

G

A

L

M

V

G

T

T

N

S

L

L

G

G

I

L

G

A

S

T

L

P

Q

L

V

V

S

T

S

E

G

C

L

I

E

Q

N

Y

L

L

F

F

G

G

M

I

E

P

H

H

S

T

N

L

T

Q

N

L

L

D

L

A

I

I

V

I

I

I

I

S

V

C

M

W

S

I

T

L

V

L

A

N

T

A

I

I

A

C

A

V

V

A

S

F

G

G

V

L

E

Q

N

K

G

G

I

V

R

K

R

I

L

A

S

S

N

D

L

V

N
x
R

I

T

V

Y

L

L

F

S

S

F

G

L

L

M

L

L

I

G

F

W

V

V

L

F

L

I

F

V

G

G

P

G

T

A

L

S

H

F

L

I

L

V

N

N

G

Y

F

F

V

T

Q

D

N

S

I

V

G

G

D

T

Y

-

L

-

N

-

G

-

V

-

V

L

L

L

K

M

T

Y

F

M

D

P

L

R

Y

M

V

L

Y

F

E

Y

G

T

D

D

S

P

E

I

K

G

S

K

D

G

R

G

W

F

L

P

G

Q

L

A

W
|
W

T

T

L

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

A

A

P

I

F

Q

V
x
M

G

S

M

I

F

F

I

L

A

A

R

R

I

I

S

S

R

K

G

G

R

R

T

T

V

V

R

R

E

E

L

L

V

C

A

L

G

G

V

M

L

V

L

S

-

G

I

L

P

T

L

A

G

G

F

T

T

W

L

L

A

I

W

W

L

-

S

T

I

I

F

L

G

G

N

G

S

N

A

T

L

L

D

Q

L

L

V

I

M

-

N

-

H

-

G

-

A

-

V

-

E

-

L

-

G

-

K

-

T

-

A

-

L

-

E

D

Q

Q

P

N

S

I

M

L

A

N

I

I

Y

P

Q

Q

L

L

L

I

E

D

H

Q

Y

Y

-

G

-

V

-

P

-

R

-

A

-

I

-

E

-

T

-

W

-

A

-

L

P

P

A

L

S

S

K

T

I

A

V

T

I

M

G

W

V

G

S

F

I

F

F

I

V

L

G

C

F

F

V

I

L

A

F

T

L

V

T

T

P

L

A

I

D

N

S

A

G

C

A

S

V

Y

M

T

M

L

A

A

N

M

L

S

S

T

C

C

K

R

G

S

G

M

N

K

V

E

D

G

E

A

D

E

A

P

P

P

H

L

W

L

L

V

R

R

I

I

F

G

W

W

S

S

A

V

V

L

I

V

T

G

L

I

V

I

T

G

I

I

G

I

L

L

F

A

A

L

G

G

N

G

F

L

E

K

A

P

M

I

Q

Q

T

T

M

A

V

I

L

A

A

G

G

G

L

C

P

P

E111 (= E119) mutation to A: Abolishes transport activity.
R262 (≠ N268) mutation R->A,E: Strong decrease in L-carnitine transport. Mutant is Na(+)-dependent for substrate binding and transport.
W316 (= W327) mutation to A: 2.5-fold decrease in Vmax.
M331 (≠ V342) mutation to V: 10-fold decrease in Vmax.

Query Sequence

>Pf1N1B4_2419 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2419
MSSASLIKTPPEKVTVNGWVFYTSTALILLLTAILIIAPQEAGRMLGIAQAWLSRSFGWY
YMVVIAAYLVFVVGLAFSSYGKLKLGSKDDTPDFSYGAWAGMLFSSGIGISLLYFGASEP
LDHYFNPPEGVAGSNLAARQAVQLTFLHWGLHGWAIYALVGLAVAYFAYRHNQPLALRSA
LYPLVGERWVKGAAGHAVDGFGMFVTLLGLVTNLGIGSLQVSSGLENLFGMEHSNTNLLI
VIIVMSTVATIAAVSGVENGIRRLSNLNIVLFSGLLIFVLLFGPTLHLLNGFVQNIGDYL
NGVVLKTFDLYVYEGDSEKSDRWLGLWTLFYWAWWISWAPFVGMFIARISRGRTVRELVA
GVLLIPLGFTLAWLSIFGNSALDLVMNHGAVELGKTALEQPSMAIYQLLEHYPASKIVIG
VSIFVGFVLFLTPADSGAVMMANLSCKGGNVDEDAPHWLRIFWSAVITLVTIGLLFAGNF
EAMQTMVVLAGLPFSVVLVFFMFGLHKAMRQDVQIEQEQAELAARGRRGFSERLTQLDLQ
PNQSIVQRFMDKRVSPALEDAAVQLRAQGLDVQTLLGKAKRCMGVRIEMEEGNPFVYEVS
LDGYLAAASDTVSAESADEPRARYYRAEVYLHNGSQDYDLMGFTQDQITRDVLDQFESHR
QLLGRVYS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory