SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_2440 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2440 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

7bkbB Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
29% identity, 16% coverage: 518:623/649 of query aligns to 148:259/296 of 7bkbB

query
sites
7bkbB

G

G

S

A

S

K

V

V

T

A

Y

A

H

H

D

Y

P
x
G

C
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C

Y

H

L

L

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M

-

K

-

P

-

K

G

E

R

R

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H

N

F

G

G

E

D

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T

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E

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N

P

P

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M

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W

-

F

R

E

E

E

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L

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R

G

A

A

L

L

G

G

I

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E

E

I

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K

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E

Q

M

Y

Q

R

R

N

S

-

G

-

F

-

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K

S

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R

Q

C
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C

G

G

G

A

G

G

A

V

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R

I

G

T

Y

D

D

I

I

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V

G

H

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Q

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I

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D

N

M

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M

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N

I

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Q

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E

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A

G

G

A

A

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A

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L

C
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C

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P

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F

C
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C

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Q

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L

M

Q

L

F

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D

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R

G

G

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I

binding Non-cubane [4Fe-4S]-cluster: G156 (≠ P526), C157 (= C527), C197 (= C562), C198 (= C563), C235 (= C600), F237 (≠ Q602), F237 (≠ Q602), C238 (= C603)

Sites not aligning to the query:

binding Non-cubane [4Fe-4S]-cluster: 9, 10, 42, 43, 79, 82

Query Sequence

>Pf1N1B4_2440 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2440
MLNTLLPILLFAALGLAVLGALRRVAMWRQGRASKVDLIGGLFAMPKRYMVDLHHVVARD
KYIANTHVATAGGAVASIVLAILVHGFGLHNRILGYALLLMSAVMFVGAIFMFLRRRNPP
ARLSKGPWMRLPKSLMAFSASFFLLTLPVAGILPENFGGWVVAAILGVGVLWGVSELFFG
MTWGGPMKHAFAGALHLAWHRRAERFGGGRSTGLKPLDLNDPNAPLGVEKPKDFTWNQLL
GFDACVQCGKCEAACPAFAAGQPLNPKKLIQDMVVGLAGGTDAKFAGRPYPGKAIGEHGG
NPHQPIVNGLVDAETLWSCTTCRACVEECPMMIEHVDAIVDMRRHLTLEKGATPNKGAEV
LENLIATDNPGGFAPGGRMNWAADLNLNLLSEKKSTDVLFWVGDGAFDMRNQRTLRAFVK
VLKAAKVDFAVLGLEERDSGDVARRLGDEATFQLLAKRNIQTLAKYSFNRIVTCDPHSFH
VLKNEYGAFDGNYLVQHHSTYMAEIIGEGALNLGQHKGSSVTYHDPCYLGRYNGEYEAPR
EVLRALGIEIKEMQRSGFRSRCCGGGGGAPITDIPGKQRIPDMRMEDIRETGAELVAVGC
PQCTAMLEGVVEPRPLIKDIAELVADALLEDAAPGKPSAPAKREPAEVH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory