SitesBLAST
Comparing Pf1N1B4_2505 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2505 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5yb6A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- lysine complex (see paper)
91% identity, 99% coverage: 5:560/560 of query aligns to 1:556/556 of 5yb6A
- active site: E241 (= E245), K361 (= K365)
- binding flavin-adenine dinucleotide: V47 (= V51), G48 (= G52), I51 (= I55), A52 (= A56), E71 (= E75), A72 (= A76), G77 (= G81), R78 (= R82), L79 (= L83), G95 (= G99), G96 (= G100), R98 (= R102), F99 (= F103), V297 (= V301), L331 (= L335), I336 (= I340), S359 (= S363), W459 (= W463), F465 (= F469), A468 (= A472), F469 (= F473), G503 (= G507), D504 (= D508), A511 (= A515), W512 (= W516), V513 (= V517), A516 (= A520)
- binding lysine: R98 (= R102), W231 (= W235), D234 (= D238), T333 (= T337), W348 (= W352), D352 (= D356), R390 (= R394), L391 (= L395), Y412 (= Y416), W414 (= W418), A511 (= A515), W512 (= W516)
5yb8A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- arginine complex (see paper)
91% identity, 98% coverage: 12:560/560 of query aligns to 1:549/549 of 5yb8A
- active site: E234 (= E245), K354 (= K365)
- binding arginine: R91 (= R102), D227 (= D238), E333 (= E344), Q338 (= Q349), D345 (= D356), D382 (= D393), R383 (= R394), Y405 (= Y416), W407 (= W418), A504 (= A515), W505 (= W516)
- binding flavin-adenine dinucleotide: V40 (= V51), G41 (= G52), G43 (= G54), I44 (= I55), A45 (= A56), E64 (= E75), A65 (= A76), G70 (= G81), R71 (= R82), L72 (= L83), G88 (= G99), G89 (= G100), M90 (= M101), R91 (= R102), F92 (= F103), G289 (= G300), V290 (= V301), C320 (= C331), L324 (= L335), I329 (= I340), W452 (= W463), F458 (= F469), A461 (= A472), F462 (= F473), D497 (= D508), A504 (= A515), W505 (= W516), V506 (= V517)
5yb7A L-amino acid oxidase/monooxygenase from pseudomonas sp. Aiu 813 - l- ornithine complex (see paper)
91% identity, 98% coverage: 12:560/560 of query aligns to 1:549/549 of 5yb7A
- active site: E234 (= E245), K354 (= K365)
- binding flavin-adenine dinucleotide: V40 (= V51), G41 (= G52), G43 (= G54), I44 (= I55), A45 (= A56), E64 (= E75), A65 (= A76), G70 (= G81), R71 (= R82), L72 (= L83), G88 (= G99), G89 (= G100), R91 (= R102), F92 (= F103), V290 (= V301), C320 (= C331), L324 (= L335), I329 (= I340), W452 (= W463), F458 (= F469), A461 (= A472), F462 (= F473), G496 (= G507), D497 (= D508), A504 (= A515), W505 (= W516), V506 (= V517)
- binding L-ornithine: R91 (= R102), D227 (= D238), T326 (= T337), E333 (= E344), Q338 (= Q349), W341 (= W352), D345 (= D356), R383 (= R394), L384 (= L395), Y405 (= Y416), A504 (= A515), W505 (= W516)
4iv9A Structure of the flavoprotein tryptophan-2-monooxygenase (see paper)
31% identity, 92% coverage: 30:544/560 of query aligns to 18:542/552 of 4iv9A
- active site: E249 (= E245), K360 (= K365)
- binding flavin-adenine dinucleotide: G40 (= G52), G42 (= G54), S44 (≠ A56), E64 (= E75), S65 (≠ A76), R66 (≠ S77), G71 (= G81), R72 (= R82), G90 (= G99), A91 (≠ G100), M92 (= M101), R93 (= R102), V295 (= V301), W461 (= W463), A470 (= A472), G505 (= G507), C506 (≠ D508), G513 (≠ A515), W514 (= W516), I515 (≠ V517), A518 (= A520)
- binding 2-(1h-indol-3-yl)acetamide: F239 (≠ W235), M253 (≠ V249), W410 (= W418), F471 (= F473), G513 (≠ A515), W514 (= W516)
P06617 Tryptophan 2-monooxygenase; EC 1.13.12.3 from Pseudomonas savastanoi (Pseudomonas syringae pv. savastanoi) (see paper)
31% identity, 92% coverage: 30:544/560 of query aligns to 23:547/557 of P06617
- K365 (= K365) mutation to M: Abolishes catalytic activity.
- W466 (= W463) mutation to F: Slightly decreases enzyme activity.; mutation to M: Abolishes flavin binding.
3kveA Structure of native l-amino acid oxidase from vipera ammodytes ammodytes: stabilization of the quaternary structure by divalent ions and structural changes in the dynamic active site
24% identity, 87% coverage: 48:533/560 of query aligns to 34:480/484 of 3kveA
- active site: P90 (= P104), H221 (vs. gap), K324 (= K365)
- binding flavin-adenine dinucleotide: V37 (= V51), G38 (= G52), G40 (= G54), S42 (≠ A56), L60 (≠ Y74), E61 (= E75), A62 (= A76), G68 (= G81), R69 (= R82), G85 (= G99), P86 (≠ G100), M87 (= M101), R88 (= R102), R258 (≠ G300), V259 (= V301), T292 (= T329), Y370 (= Y416), W418 (= W463), Y423 (≠ H468), I428 (≠ F473), G454 (= G507), E455 (≠ D508), G462 (≠ A515), W463 (= W516), I464 (≠ V517), T467 (≠ A520)
P0DI84 L-amino-acid oxidase; LAO; VAA-LAAO I; EC 1.4.3.2 from Vipera ammodytes ammodytes (Western sand viper) (see paper)
24% identity, 87% coverage: 48:533/560 of query aligns to 34:480/484 of P0DI84
- MS 41:42 (≠ IA 55:56) binding
- EA 61:62 (= EA 75:76) binding
- R69 (= R82) binding
- H73 (≠ Q86) binding
- GPMR 85:88 (≠ GGMR 99:102) binding
- N170 (≠ R187) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- C171 (≠ L188) modified: Disulfide link with 8
- V259 (= V301) binding
- E277 (vs. gap) binding
- C329 (≠ V370) modified: Disulfide link with 410
- C410 (≠ I454) modified: Disulfide link with 329
- E455 (≠ D508) binding
- GWIDST 462:467 (≠ AWVEGA 515:520) binding
Sites not aligning to the query:
- 8 modified: Disulfide link with 171
G8XQX1 L-amino-acid oxidase; DrLAO; LAAO; EC 1.4.3.2 from Daboia russelii (Russel's viper) (Vipera russelii) (see paper)
24% identity, 93% coverage: 9:527/560 of query aligns to 19:494/504 of G8XQX1
- H241 (vs. gap) mutation to A: Shows high reactivity toward L-Arg, but does not induce change toward L-Leu, L-Phe and L-Met.; mutation to N: No change in activity.; mutation to S: Shows middle reactivity toward L-Arg and L-Phe, but does not induce change toward L-Leu, and L-Met.
Sites not aligning to the query:
Q6STF1 L-amino-acid oxidase; AHP-LAAO; LAO; EC 1.4.3.2 from Gloydius halys (Chinese water mocassin) (Agkistrodon halys) (see paper)
22% identity, 88% coverage: 39:533/560 of query aligns to 45:500/504 of Q6STF1
- MS 61:62 (≠ IA 55:56) binding
- EA 81:82 (= EA 75:76) binding
- R89 (= R82) binding
- GPMR 105:108 (≠ GGMR 99:102) binding
- R108 (= R102) binding
- N190 (≠ L195) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- C191 (≠ V196) modified: Disulfide link with 28
- H241 (vs. gap) binding
- V279 (≠ L289) binding
- C349 (≠ V370) modified: Disulfide link with 430
- N379 (≠ D406) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- Y390 (= Y416) binding
- C430 (≠ I454) modified: Disulfide link with 349
- E475 (≠ D508) binding
- GWIDST 482:487 (≠ AWVEGA 515:520) binding
Sites not aligning to the query:
- 1:18 signal peptide
- 28 modified: Disulfide link with 191
1tdoA L-amino acid oxidae from agkistrodon halys in complex with l- phenylalanine
22% identity, 88% coverage: 39:533/560 of query aligns to 25:480/484 of 1tdoA
- active site: P90 (= P104), H221 (vs. gap), K324 (= K365)
- binding flavin-adenine dinucleotide: V37 (= V51), G38 (= G52), G40 (= G54), S42 (≠ A56), L60 (≠ Y74), E61 (= E75), A62 (= A76), G68 (= G81), R69 (= R82), G85 (= G99), P86 (≠ G100), M87 (= M101), R88 (= R102), Q258 (≠ S288), V259 (≠ L289), T292 (= T329), Y370 (= Y416), W418 (= W463), Y423 (≠ H468), G427 (≠ A472), I428 (≠ F473), E455 (≠ D508), G462 (≠ A515), W463 (= W516), I464 (≠ V517), T467 (≠ A520)
- binding phenylalanine: R88 (= R102), N206 (≠ F230), H221 (vs. gap), F225 (= F247), Y354 (= Y400), Y370 (= Y416)
1tdnA L-amino acid oxidase from agkistrodon halys in complex with l-leucine
22% identity, 88% coverage: 39:533/560 of query aligns to 25:480/484 of 1tdnA
- active site: P90 (= P104), H221 (vs. gap), K324 (= K365)
- binding flavin-adenine dinucleotide: G38 (= G52), G40 (= G54), S42 (≠ A56), L60 (≠ Y74), E61 (= E75), A62 (= A76), G68 (= G81), R69 (= R82), G85 (= G99), P86 (≠ G100), M87 (= M101), R88 (= R102), Q258 (≠ S288), V259 (≠ L289), T292 (= T329), Y370 (= Y416), Y423 (≠ H468), G427 (≠ A472), I428 (≠ F473), E455 (≠ D508), G462 (≠ A515), W463 (= W516), I464 (≠ V517), T467 (≠ A520)
- binding leucine: R88 (= R102), N206 (≠ F230), F225 (= F247), Y370 (= Y416)
1tdkA L-amino acid oxidase from agkistrodon halys in complex with suicide substrate l-vinylglycine
22% identity, 88% coverage: 39:533/560 of query aligns to 25:480/484 of 1tdkA
- active site: P90 (= P104), H221 (vs. gap), K324 (= K365)
- binding flavin-adenine dinucleotide: V37 (= V51), G38 (= G52), G40 (= G54), S42 (≠ A56), L60 (≠ Y74), E61 (= E75), A62 (= A76), G68 (= G81), R69 (= R82), G85 (= G99), P86 (≠ G100), R88 (= R102), Q258 (≠ S288), V259 (≠ L289), T292 (= T329), G427 (≠ A472), I428 (≠ F473), E455 (≠ D508), G462 (≠ A515), W463 (= W516), I464 (≠ V517), T467 (≠ A520)
- binding l-vinylglycine: R88 (= R102), R88 (= R102), N206 (≠ F230), H221 (vs. gap), Y354 (= Y400), Y370 (= Y416)
1reoA L-amino acid oxidase from agkistrodon halys pallas (see paper)
22% identity, 88% coverage: 39:533/560 of query aligns to 25:480/484 of 1reoA
- active site: P90 (= P104), H221 (vs. gap), K324 (= K365)
- binding flavin-adenine dinucleotide: G38 (= G52), G40 (= G54), S42 (≠ A56), L60 (≠ Y74), E61 (= E75), A62 (= A76), G68 (= G81), R69 (= R82), G85 (= G99), P86 (≠ G100), R88 (= R102), Q258 (≠ S288), V259 (≠ L289), T292 (= T329), Y370 (= Y416), Y423 (≠ H468), G427 (≠ A472), I428 (≠ F473), E455 (≠ D508), G462 (≠ A515), W463 (= W516), I464 (≠ V517), T467 (≠ A520)
C0HJE7 L-amino acid oxidase bordonein-L; LAAO; LAO; EC 1.4.3.2 from Crotalus durissus terrificus (South American rattlesnake) (see paper)
22% identity, 89% coverage: 48:545/560 of query aligns to 54:506/516 of C0HJE7
- C189 (≠ V196) modified: Disulfide link with 28
- C349 (≠ V370) modified: Disulfide link with 430
- C430 (≠ I455) modified: Disulfide link with 349
Sites not aligning to the query:
- 28 modified: Disulfide link with 189
P0CC17 L-amino-acid oxidase; BatroxLAAO; LAO; EC 1.4.3.2 from Bothrops atrox (Barba amarilla) (Fer-de-lance) (see 2 papers)
22% identity, 87% coverage: 48:533/560 of query aligns to 54:500/506 of P0CC17
- E111 (≠ V105) binding
- E118 (≠ H112) binding
- N190 (≠ R187) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- C191 (≠ L188) modified: Disulfide link with 28
- D219 (≠ E223) binding
- E248 (≠ D255) binding
- E299 (vs. gap) binding
- H332 (≠ M353) binding
- C349 (≠ V370) modified: Disulfide link with 430
- C430 (≠ I455) modified: Disulfide link with 349
- H458 (= H490) binding
Sites not aligning to the query:
- 1:18 signal peptide
- 28 modified: Disulfide link with 191
- 36 binding
5ts5A Crystal structure of l-amino acid oxidase from bothrops atrox (see paper)
22% identity, 87% coverage: 48:533/560 of query aligns to 35:481/483 of 5ts5A
- active site: P91 (= P104), H222 (vs. gap), K325 (= K365)
- binding flavin-adenine dinucleotide: V38 (= V51), G39 (= G52), S43 (≠ A56), L61 (≠ Y74), E62 (= E75), A63 (= A76), G69 (= G81), R70 (= R82), G86 (= G99), P87 (≠ G100), R89 (= R102), R259 (≠ P297), V260 (≠ R298), T293 (= T329), A297 (≠ S333), Y371 (= Y416), W419 (= W463), G428 (≠ A472), I429 (≠ F473), G455 (= G507), E456 (≠ D508), G463 (≠ A515), W464 (= W516), I465 (≠ V517), T468 (≠ A520)
- binding zinc ion: H313 (≠ M353), H439 (= H490)
Q6TGQ9 L-amino-acid oxidase BjussuLAAO-I; BjsuLAAO; LAO; EC 1.4.3.2 from Bothrops jararacussu (Jararacussu) (see paper)
22% identity, 85% coverage: 48:523/560 of query aligns to 49:485/497 of Q6TGQ9
- C186 (≠ L188) modified: Disulfide link with 23
- C344 (≠ V370) modified: Disulfide link with 425
- C425 (≠ I455) modified: Disulfide link with 344
Sites not aligning to the query:
- 23 modified: Disulfide link with 186
P81382 L-amino-acid oxidase; LAO; MPV-LAAO; EC 1.4.3.2 from Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma) (see 5 papers)
23% identity, 87% coverage: 39:527/560 of query aligns to 45:494/516 of P81382
- MA 61:62 (≠ IA 55:56) binding
- EA 81:82 (= EA 75:76) binding
- R89 (= R82) binding
- GPMR 105:108 (≠ GGMR 99:102) binding
- R108 (= R102) binding
- N190 (≠ W192) modified: carbohydrate, N-linked (GlcNAc...) (complex) asparagine
- C191 (≠ N193) modified: Disulfide link with 28
- H241 (vs. gap) binding
- V279 (= V301) binding
- C349 (≠ V370) modified: Disulfide link with 430
- N379 (≠ D406) modified: carbohydrate, N-linked (GlcNAc...) (complex) asparagine
- Y390 (= Y416) binding
- C430 (≠ I454) modified: Disulfide link with 349
- E475 (≠ D508) binding
- GW 482:483 (≠ AW 515:516) binding
- GWIDST 482:487 (≠ AWVEGA 515:520) binding
Sites not aligning to the query:
- 1:18 signal peptide
- 28 modified: Disulfide link with 191
1f8sA Crystal structure of l-amino acid oxidase from calloselasma rhodostoma, complexed with three molecules of o-aminobenzoate. (see paper)
23% identity, 87% coverage: 39:527/560 of query aligns to 23:472/482 of 1f8sA
- active site: P88 (= P104), H219 (vs. gap), K322 (= K365)
- binding 2-aminobenzoic acid: R86 (= R102), Y153 (≠ Q177), L203 (≠ G229), L203 (≠ G229), N204 (≠ F230), E205 (≠ G231), E205 (≠ G231), H219 (vs. gap), H219 (vs. gap), D220 (vs. gap), R318 (≠ M361), Y368 (= Y416), I370 (≠ W418), I426 (≠ F473), G460 (≠ A515), W461 (= W516)
- binding flavin-adenine dinucleotide: V35 (= V51), G36 (= G52), G38 (= G54), A40 (= A56), L58 (≠ Y74), E59 (= E75), A60 (= A76), G66 (= G81), R67 (= R82), G83 (= G99), P84 (≠ G100), M85 (= M101), R86 (= R102), Q256 (≠ G300), V257 (= V301), T290 (= T329), W416 (= W463), Y421 (≠ H468), I426 (≠ F473), G452 (= G507), E453 (≠ D508), G460 (≠ A515), W461 (= W516), I462 (≠ V517), T465 (≠ A520)
2iidA Structure of l-amino acid oxidase from calloselasma rhodostoma in complex with l-phenylalanine (see paper)
23% identity, 87% coverage: 39:527/560 of query aligns to 24:473/483 of 2iidA
- active site: P89 (= P104), H220 (vs. gap), K323 (= K365)
- binding flavin-adenine dinucleotide: V36 (= V51), G37 (= G52), G39 (= G54), A41 (= A56), E60 (= E75), A61 (= A76), G67 (= G81), R68 (= R82), G84 (= G99), P85 (≠ G100), M86 (= M101), R87 (= R102), V258 (= V301), T291 (= T329), W417 (= W463), Y422 (≠ H468), I427 (≠ F473), G453 (= G507), E454 (≠ D508), G461 (≠ A515), W462 (= W516), I463 (≠ V517), T466 (≠ A520)
- binding phenylalanine: R87 (= R102), R319 (≠ M361), Y369 (= Y416), I371 (≠ W418), G461 (≠ A515), W462 (= W516)
Query Sequence
>Pf1N1B4_2505 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_2505
MNKNNRHPADGKKPVTVFGPDFPFAFDDWIEHPAGLGSIPAHNHGAEVAIVGAGIAGLVA
AYELMKLGLKPVVYEASKMGGRLRSQAFNGAEGIIAELGGMRFPVSSTAFYHYVDKLGLQ
TKPFPNPLTPASGSTVIDLEGKTHYAQKLADLPALFQEVADAWADALEAGSQFSDIQQAI
RDRDVPRLKELWNTLVPLWDDRTFYDFVATSEAFAKLSFHHREVFGQVGFGTGGWDSDFP
NSMLEIFRVVMTNCDDHQHLVVGGVEQVPLGIWRHVPERCVHWPEGTSLSSLHSGAPRSG
VKKIAHAPNGRFTVTDNYGDTREYAAVLTTCQSWLLTTQIECDESLFSQKMWMALDRTRY
MQSSKTFVMVDRPFWKDKDPETGRDLMSMTLTDRLTRGTYLFDNGDDKPGVICLSYSWMS
DALKMLPHPVEKRVKLALDALKKIYPKVDIAARIIGDPITVSWEADPHFLGAFKGALPGH
YRYNQRMYAHFMQDDMPAEQRGIFIAGDDVSWTPAWVEGAVQTSLNAVWGIMKHFGGETH
AENPGPGDVFNEIGPIALPE
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory