SitesBLAST

C26 (≠ Q40) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F41) mutation to L: Lower ATPase activity and transport efficiency.
F45 (= F59) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S68) mutation to T: Loss of ATPase activity and transport.
L60 (= L74) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ I90) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V149) mutation to M: Loss of ATPase activity and transport.
D172 (= D186) mutation to N: Loss of ATPase activity and transport.
C276 (vs. gap) mutation to A: Lower ATPase activity and transport efficiency.
E297 (= E288) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.

2d62A Crystal structure of multiple sugar binding transport atp-binding protein
55% identity, 63% coverage: 32:264/372 of query aligns to 7:245/375 of 2d62A

query
sites
2d62A

V

V

Q

K

F

L

T

I

N

N

V

I

S

W

R

K

Q

R

F

F

G

G

E

D

V

V

K

T

A

A

V

V

D

K

R

D

V

L

S

S

I

L

D

E

I

I

Q

K

D

D

G

G

E

E

F

F

F

L

S

V

M

L

L

L

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

C

T

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

P

S

T

A

R

G

G

S

Q

I

I

R

Y

I

I

H

E

G

D

A

N

E

L

A

V

A

A

G

D

-

P

-

E

-

K

-

G

-

V

-

F

L

V

P

P

Y

K

Q

E

R

R

D

D

V

V

N

A

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

Y

D

D

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

V

L

K

R

G

K

V

V

G

P

K

K

T

Q

E

E

R

I

Q

D

K

K

R

R

A

V

E

R

E

E

A

V

L

A

D

E

M

M

V

L

A

G

L

L

G

T

G

E

Y

L

G

L

E

N

R

R

K

K

P

P

V

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

V

I

N

R

R

R

P

P

R

K

V

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

K

M

M

Q

R

G

A

E

E

L

L

K

K

L

L

Q

Q

R

R

Q

Q

L

L

G

G

I

V

T

T

F

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

A

A

V

V

F

M

N

N

K

K

G

G

R

E

I

L

E

Q

Q

Q

V

V

D

G

T

T

P

P

R

D

N

E

L

V

Y

Y

M

Y

K

K

P

P

A

V

T

N

T

T

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding pyrophosphate 2-: G42 (= G67), C43 (≠ S68), G44 (= G69), K45 (= K70), T46 (= T71), T47 (= T72)

1g291 Malk (see paper)
53% identity, 63% coverage: 31:264/372 of query aligns to 3:242/372 of 1g291

query
sites
1g291

A

G

V

V

Q

R

F

L

T

V

N

D

V

V

S

W

R

K

Q

V

F

F

G

G

E

E

V

V

K

T

A

A

V

V

D

R

R

E

V

M

S

S

I

L

D

E

I

V

Q

K

D

D

G

G

E

E

F

F

F

M

S

I

M

L

L

L

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

C

T

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

P

S

S

A

R

G

G

S

Q

I

I

R

Y

I

I

H

G

G

D

A

K

E

L

A

V

A

A

G
x
D

-

P

-
x
E

-
x
K

-

G

-

I

-

F

L

V

P

P

Y
x
K

Q
x
D

R

R

D

D

V

I

N

A

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

Y

D

D

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

V

L

K

R

G

K

V

V

G

P

K

R

T

Q

E

E

R

I

Q

D

K

Q

R

R

A

V

E

R

E

E

A

V

L

A

D

E

M

L

V

L

A

G

L

L

G

T

G

E

Y

L

G

L

E

N

R

R

K

K

P

P

V

R

Q

E

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

L

A

G

R

R

A

A

L

I

V

V

N

R

R

K

P

P

R

Q

V

V

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

R

M

M

Q

R

G

A

E

E

L

L

K

K

L

L

Q

Q

R

R

Q

Q

L

L

G

G

I

V

T

T

F

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

A

A

V

V

F

M

N

N

K

R

G

G

R

V

I

L

E

Q

Q

Q

V

V

D

G

T

S

P

P

R

D

N

E

L

V

Y

Y

M

D

K

K

P

P

A

A

T

N

T

T

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding magnesium ion: D69 (≠ G97), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y101), D80 (≠ Q102)
binding pyrophosphate 2-: S38 (= S66), G39 (= G67), C40 (≠ S68), G41 (= G69), K42 (= K70), T43 (= T71), T44 (= T72)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
44% identity, 70% coverage: 32:290/372 of query aligns to 7:278/353 of 1vciA

query
sites
1vciA

V

V

Q

K

F

L

T

E

N

N

V

L

S

T

R

K

Q

R

F
|
F

G

G

E

N

V
x
F

K

T

A

A

V

V

D

N

R

K

V

L

S

N

I

L

D

T

I

I

Q

K

D

D

G

G

E

E

F

F

F

L

S

V

M

L

L

L

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
|
T

C

T

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

P

S

T

A

E

G

G

S

R

I

I

R

Y

I

F

H

G

G

D

A

R

E

D

A

V

A

T

G

Y

L

L

P

P

Y

K

Q

D

R

R

D

N

V

I

N

S

T

M

V

V

F

F

Q

Q

D

-

Y

-

A

-

L

-

F

-

P

-

H

H

M

M

N

T

V

V

L

Y

D

E

N

N

V

I

A

A

Y

F

G

P

L

L

K

K

V

-

K

-

G

K

V

F

G

P

K

K

T

D

E

E

R

I

Q

D

K

K

R

R

A

V

E

R

E

W

A

A

L

A

D

E

M

L

V

L

A

Q

L

I

G

E

Y

L

G

L

E

N

R

R

K

Y

P

P

V

A

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

V

A

A

R

R

A

A

L

I

V

V

N

V

R

E

P

P

R

D

V

V

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

A

M

M

Q

R

G

A

E

E

L

I

K

K

L

L

Q

Q

R

Q

Q

K

L

L

G

K

I

V

T

T

F

T

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

M

S

G

D

D

R

R

V

I

A

A

V

V

F

M

N

N

K

R

G

G

R

Q

I

L

E

L

Q

Q

V

I

D

G

T

S

P

P

R

T

N

E

L

V

Y

Y

M

L

K

R

P

P

A

N

T

S

T

V

F

F

V

V

A

A

E

T

F

F

V

I

G

G

T

A

S

P

-

E

-

M

N

N

V

I

I

L

-

E

-

V

-

S

-

V

-

G

-

D

-

G

-

Y

-

L

-

E

-

G

R

R

G

G

-

F

-

R

-

I

-

E

-

L

-

P

-

Q

-

M

D

D

L

L

A

L

Q

K

Q

D

L

Y

S

V

G

G

N

K

P

T

Q

V

P

L

F

F

S

G

I

I

R

R

P

P

E

E

H

H

V

M

binding adenosine-5'-triphosphate: F16 (= F41), F19 (≠ V44), S41 (= S66), G42 (= G67), G44 (= G69), K45 (= K70), T46 (= T71), T47 (= T72)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
46% identity, 64% coverage: 31:267/372 of query aligns to 3:239/369 of P19566

query
sites
P19566

A

S

V

V

Q

Q

F

L

T

R

N

N

V

V

S

T

R

K

Q

A

F

W

G

G

E

D

V

V

K

V

A

V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

D

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

T

E

R

A

M

A

N

G

D

L

I

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

M

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

E

R

R

K

K

P

P

V

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

R

V

V

L

F

L

L

D

D

E

E

P
|
P

L

L

G

S

A

N

L

L

D
|
D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

S

P

N

K

V

M

L86 (= L114) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P188) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D193) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 63% coverage: 31:264/372 of query aligns to 3:236/371 of P68187

query
sites
P68187

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F

W

G

G

E

E

V

V

K

V

A

V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

S

T

T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G
x
K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A
x
V

E

N

E

Q

A
x
V

L

A

D
x
E

M

V

V

L

A

Q

L

L

G
x
A

G

H

Y

L

G

L

E

D

R

R

K

K

P

P

V

K

Q

A

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D
|
D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T
x
R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

A85 (= A113) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ G134) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A142) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ A145) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ D147) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G152) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G165) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D186) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ T256) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
46% identity, 63% coverage: 31:264/372 of query aligns to 2:235/371 of 3puyA

query
sites
3puyA

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A

V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F41), S37 (= S66), G38 (= G67), C39 (≠ S68), G40 (= G69), K41 (= K70), S42 (≠ T71), T43 (= T72), Q81 (= Q110), R128 (= R157), A132 (≠ Q161), S134 (= S163), G136 (= G165), Q137 (= Q166), E158 (= E187), H191 (= H220)
binding magnesium ion: S42 (≠ T71), Q81 (= Q110)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
46% identity, 63% coverage: 31:264/372 of query aligns to 2:235/371 of 3puxA

query
sites
3puxA

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A

V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

K

Q

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-diphosphate: W12 (≠ F41), G38 (= G67), C39 (≠ S68), G40 (= G69), K41 (= K70), S42 (≠ T71), T43 (= T72), R128 (= R157), S134 (= S163), Q137 (= Q166)
binding beryllium trifluoride ion: S37 (= S66), G38 (= G67), K41 (= K70), Q81 (= Q110), S134 (= S163), G136 (= G165), H191 (= H220)
binding magnesium ion: S42 (≠ T71), Q81 (= Q110)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
46% identity, 63% coverage: 31:264/372 of query aligns to 2:235/371 of 3puwA

query
sites
3puwA

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A
x
V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

K

Q
x
A

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E
|
E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-diphosphate: W12 (≠ F41), V17 (≠ A46), G38 (= G67), C39 (≠ S68), G40 (= G69), K41 (= K70), S42 (≠ T71), T43 (= T72), R128 (= R157), A132 (≠ Q161), S134 (= S163), Q137 (= Q166)
binding tetrafluoroaluminate ion: S37 (= S66), G38 (= G67), K41 (= K70), Q81 (= Q110), S134 (= S163), G135 (= G164), G136 (= G165), E158 (= E187), H191 (= H220)
binding magnesium ion: S42 (≠ T71), Q81 (= Q110)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
46% identity, 63% coverage: 31:264/372 of query aligns to 2:235/371 of 3puvA

query
sites
3puvA

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A
x
V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

K

Q
x
A

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-diphosphate: W12 (≠ F41), V17 (≠ A46), G38 (= G67), C39 (≠ S68), G40 (= G69), K41 (= K70), S42 (≠ T71), T43 (= T72), R128 (= R157), A132 (≠ Q161), S134 (= S163), Q137 (= Q166)
binding magnesium ion: S42 (≠ T71), Q81 (= Q110)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
46% identity, 63% coverage: 31:264/372 of query aligns to 2:235/374 of 2awnB

query
sites
2awnB

A

S

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A
x
V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R

R

K

K

P

P

V

K

Q

A

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-diphosphate: W12 (≠ F41), V17 (≠ A46), S37 (= S66), G38 (= G67), G40 (= G69), K41 (= K70), S42 (≠ T71), T43 (= T72)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
45% identity, 63% coverage: 32:267/372 of query aligns to 1:236/367 of 1q12A

query
sites
1q12A

V

V

Q

Q

F

L

T

Q

N

N

V

V

S

T

R

K

Q

A

F
x
W

G

G

E

E

V

V

K

V

A

V

V

S

D

K

R

D

V

I

S

N

I

L

D

D

I

I

Q

H

D

E

G

G

E

E

F

F

F

V

S

V

M

F

L

V

G

G

P

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

L

L

L

R

R

L

M

I

I

A

A

G

G

F

L

E

E

Q

T

P

I

S

T

A

S

G

G

S

D

I

L

R

F

I

I

H

G

G

E

A

K

E

R

A

M

A

N

G

D

L

T

P

P

Y

A

Q

E

R

R

D

G

V

V

N

G

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

L

N

S

V

V

L

A

D

E

N

N

V

M

A

S

Y

F

G

G

L

L

K

K

V

L

K

A

G

G

V

A

G

K

K

K

T

E

E

V

R

I

Q

N

K

Q

R

R

A

V

E

N

E

Q

A

V

L

A

D

E

M

V

V

L

A

Q

L

L

G

A

G

H

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

K

Q
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

I

A

G

R

R

A

T

L

L

V

V

N

A

R

E

P

P

R

S

V

V

L

F

L

L

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

A

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

I

E

E

L

I

K

S

K

R

L

L

Q

H

R

K

Q

R

L

L

G

G

I

R

T

T

F

M

I

I

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

V

E

E

A

A

L

M

S

T

M

L

S

A

D

D

R

K

V

I

A

V

V

V

F

L

N

D

K

A

G

G

R

R

I

V

E

A

Q

Q

V

V

D

G

T

K

P

P

R

L

N

E

L

L

Y

Y

M

H

K

Y

P

P

A

A

T

D

T

R

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

S

P

N

K

V

M

binding adenosine-5'-triphosphate: W10 (≠ F41), S35 (= S66), G36 (= G67), C37 (≠ S68), G38 (= G69), K39 (= K70), S40 (≠ T71), T41 (= T72), R126 (= R157), A130 (≠ Q161), S132 (= S163), G134 (= G165), Q135 (= Q166)

8hprC Lpqy-sugabc in state 4 (see paper)
47% identity, 61% coverage: 37:264/372 of query aligns to 8:236/363 of 8hprC

query
sites
8hprC

V

V

S

T

R

K

Q

S

F
x
Y

G

P

E

D

V

V

K

R

A

A

-

A

V

V

D

K

R

E

V

F

S

S

I

M

D

T

I

I

Q

A

D

D

G

G

E

E

F

F

F

I

S

I

M

L

L

V

G

G

P

P

S
|
S

G
|
G

S

C

G
|
G

K
|
K

T
x
S

T

T

C

T

L

L

R

N

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

I

S

T

A

S

G

G

S

E

I

L

R

R

I

I

H

G

G

G

A

E

E

R

A

V

A

N

G

E

L

K

P

A

P

P

Y

K

Q

D

R

R

D

D

V

I

N

A

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

V

L

K

A

G

K

V

V

G

P

K

K

T

A

E

E

R

I

Q

A

K

A

R

K

A

V

E

E

A

T

L

A

D

K

M

I

V

L

A

D

L

L

G

S

G

E

Y

L

G

L

E

D

R

R

K

K

P

P

V

G

Q
|
Q

L

L

S

S

G
|
G

Q
|
Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

N

R

R

S

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

A

E

E

L

I

K

S

K

R

L

L

Q

Q

R

D

Q

R

L

L

G

G

I

T

T

T

F

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

A

V

V

V

F

M

N

L

K

A

G

G

R

E

I

V

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

D

N

E

L

L

Y

Y

M

S

K

S

P

P

A

A

T

N

T

L

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-triphosphate: Y12 (≠ F41), S38 (= S66), G39 (= G67), G41 (= G69), K42 (= K70), S43 (≠ T71), Q82 (= Q110), Q133 (= Q161), G136 (= G164), G137 (= G165), Q138 (= Q166), H192 (= H220)
binding magnesium ion: S43 (≠ T71), Q82 (= Q110)

8hprD Lpqy-sugabc in state 4 (see paper)
47% identity, 61% coverage: 37:264/372 of query aligns to 8:236/362 of 8hprD

query
sites
8hprD

V

V

S

T

R

K

Q

S

F
x
Y

G

P

E

D

V

V

K

R

A

A

-

A

V

V

D

K

R

E

V

F

S

S

I

M

D

T

I

I

Q

A

D

D

G

G

E

E

F

F

F

I

S

I

M

L

L

V

G

G

P

P

S
|
S

G

G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

T

L

L

R

N

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

I

S

T

A

S

G

G

S

E

I

L

R

R

I

I

H

G

G

G

A

E

E

R

A

V

A

N

G

E

L

K

P

A

P

P

Y

K

Q

D

R

R

D

D

V

I

N

A

T

M

V

V

F

F

Q
|
Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

V

L

K

A

G

K

V

V

G

P

K

K

T

A

E

E

R

I

Q

A

K

A

R

K

A

V

E

E

A

T

L

A

D

K

M

I

V

L

A

D

L

L

G

S

G

E

Y

L

G

L

E

D

R
|
R

K

K

P

P

V

G

Q
|
Q

L

L

S
|
S

G
|
G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

N

R

R

S

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E
x
Q

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

A

E

E

L

I

K

S

K

R

L

L

Q

Q

R

D

Q

R

L

L

G

G

I

T

T

T

F

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

A

V

V

V

F

M

N

L

K

A

G

G

R

E

I

V

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

D

N

E

L

L

Y

Y

M

S

K

S

P

P

A

A

T

N

T

L

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-triphosphate: Y12 (≠ F41), S38 (= S66), C40 (≠ S68), G41 (= G69), K42 (= K70), S43 (≠ T71), T44 (= T72), Q82 (= Q110), R129 (= R157), Q133 (= Q161), S135 (= S163), G136 (= G164), G137 (= G165), Q159 (≠ E187), H192 (= H220)
binding magnesium ion: S43 (≠ T71), Q82 (= Q110)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
49% identity, 59% coverage: 46:264/372 of query aligns to 19:237/393 of P9WQI3

query
sites
P9WQI3

A

A

V

V

D

R

R

D

V

L

S

N

I

L

D

T

I

I

Q

A

D

D

G

G

E

E

F

F

F

L

S

I

M

L

L

V

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

C

T

L

L

R

N

L

M

I

I

A

A

G

G

F

L

E

E

Q

D

P

I

S

S

A

S

G

G

S

E

I

L

R

R

I

I

H

A

G

G

A

E

E

R

A

V

A

N

G

E

L

K

P

A

P

P

Y

K

Q

D

R

R

D

D

V

I

N

A

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

V

L

K

A

G

K

V

M

G

R

K

K

T

A

E

D

R

I

Q

A

K

Q

R

K

A

V

E

S

E

E

A

T

L

A

D

K

M

I

V

L

A

D

L

L

G

T

G

N

Y

L

G

L

E

D

R

R

K

K

P

P

V

S

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

N

R

R

H

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E

E

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

G

E

E

L

I

K

A

K

Q

L

L

Q

Q

R

R

Q

R

L

L

G

G

I

T

T

T

F

T

I

V

F

Y

V

V

T

T

H
|
H

D

D

Q

Q

T

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

A

V

V

V

F

M

N

Y

K

G

G

G

R

I

I

A

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

E

N

E

L

L

Y

Y

M

E

K

R

P

P

A

A

T

N

T

L

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

H193 (= H220) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

8hplC Lpqy-sugabc in state 1 (see paper)
48% identity, 59% coverage: 46:264/372 of query aligns to 16:234/384 of 8hplC

query
sites
8hplC

A

A

V

V

D

K

R

E

V

F

S

S

I

M

D

T

I

I

Q

A

D

D

G

G

E

E

F

F

F

I

S

I

M

L

L

V

G

G

P

P

S

S

G
|
G

S
x
C

G
|
G

K
|
K

T
x
S

T
|
T

C

T

L

L

R

N

L

M

I

I

A

A

G

G

F

L

E

E

Q

E

P

I

S

T

A

S

G

G

S

E

I

L

R

R

I

I

H

G

G

G

A

E

E

R

A

V

A

N

G

E

L

K

P

A

P

P

Y

K

Q

D

R

R

D

D

V

I

N

A

T

M

V

V

F

F

Q

Q

D

S

Y

Y

A

A

L

L

F

Y

P

P

H

H

M

M

N

T

V

V

L

R

D

Q

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

V

L

K

A

G

K

V

V

G

P

K

K

T

A

E

E

R

I

Q

A

K

A

R

K

A

V

E

E

A

T

L

A

D

K

M

I

V

L

A

D

L

L

G

S

G

E

Y

L

G

L

E

D

R

R

K

K

P

P

V

G

Q

Q

L

L

S

S

G

G

Q

Q

R

R

Q

Q

R

R

V

V

A

A

L

M

A

G

R

R

A

A

L

I

V

V

N

R

R

S

P

P

R

K

V

A

L

F

L

L

L

M

D

D

E

Q

P

P

L

L

G

S

A

N

L

L

D

D

L

A

K

K

L

L

R

R

E

V

Q

Q

M

M

Q

R

G

A

E

E

L

I

K

S

K

R

L

L

Q

Q

R

D

Q

R

L

L

G

G

I

T

T

T

F

T

I

V

F

Y

V

V

T

T

H

H

D

D

Q

Q

T

T

E

E

A

A

L

M

S

T

M

L

S

G

D

D

R

R

V

V

A

V

V

V

F

M

N

L

K

A

G

G

R

E

I

V

E

Q

Q

Q

V

I

D

G

T

T

P

P

R

D

N

E

L

L

Y

Y

M

S

K

S

P

P

A

A

T

N

T

L

F

F

V

V

A

A

E

G

F

F

V

I

G

G

T

S

binding adenosine-5'-triphosphate: G37 (= G67), C38 (≠ S68), G39 (= G69), K40 (= K70), S41 (≠ T71), T42 (= T72)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

3d31A Modbc from methanosarcina acetivorans (see paper)
42% identity, 64% coverage: 32:270/372 of query aligns to 2:236/348 of 3d31A

query
sites
3d31A

V

I

Q

E

F

I

T

E

N

S

V

L

S

S

R

R

Q

K

F

W

G

K

E

N

V

F

K

-

A

S

V

L

D

D

R

N

V

L

S

S

I

L

D

K

I

V

Q

E

D

S

G

G

E

E

F

Y

F

F

S

V

M

I

L

L

G

G

P

P

S

T

G

G

S

A

G

G

K

K

T

T

T

L

C

F

L

L

R

E

L

L

I

I

A

A

G

G

F

F

E

H

Q

V

P

P

S

D

A

S

G

G

S

R

I

I

R

L

I

L

H

D

G

G

A

K

E

D

A

V

A

T

G

D

L

L

P

S

P

P

Y

E

Q

K

R

H

D

D

V

I

N

A

T

F

V

V

F

Y

Q

Q

D

N

Y

Y

A

S

L

L

F

F

P

P

H

H

M

M

N

N

V

V

L

K

D

K

N

N

V

L

A

E

Y

F

G

G

L

M

K

R

V

M

K

K

G

-

V

-

G

-

K

K

T

I

E

K

R

D

Q

P

K

K

R

R

A

V

E

L

E

D

A

T

L

A

D

R

M

D

V

L

A

K

L

I

G

E

G

H

Y

L

G

L

E

D

R

R

K

N

P

P

V

L

Q

T

L

L

S

S

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

N

T

R

N

P

P

R

K

V

I

L

L

D

D

E

E

P

P

L

L

G

S

A

A

L

L

D

D

L

P

K

R

L

T

R

Q

E

E

Q

N

M

A

Q

R

G

E

E

M

L

L

K

S

K

V

L

L

Q

H

R

K

Q

K

L

N

G

K

I

L

T

T

F

V

I

L

F

H

V

I

T

T

H

H

D

D

Q

Q

T

T

E

E

A

A

L

R

S

I

M

M

S

A

D

D

R

R

V

I

A

A

V

V

F

V

N

M

K

D

G

G

R

K

I

L

E

I

Q

Q

V

V

D

G

T

K

P

P

R

E

N

E

L

I

Y

F

M

E

K

K

P

P

A

V

T

E

T

G

F

R

V

V

A

A

E

S

F

F

V

V

G

G

T

F

S

E

N

N

V

V

I

L

R

K

G

G

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
37% identity, 77% coverage: 36:322/372 of query aligns to 8:315/353 of 1oxvD

query
sites
1oxvD

N

N

V

V

S

S

R

K

Q

V

F
|
F

-

K

G

G

E

K

V

V

K

V

A

A

V

L

D

D

R

N

V

V

S

N

I

I

D

N

I

I

Q

E

D

N

G

G

E

E

F

R

F

F

S

G

M

I

L

L

G

G

P

P

S
|
S

G
|
G

S

A

G
|
G

K
|
K

T
|
T

C

F

L

M

R

R

L

I

I

I

A

A

G

G

F

L

E

D

Q

V

P

P

S

S

A

T

G

G

S

E

I

L

R

Y

I

F

H

D

G

D

A

R

E

L

A

V

A

A

G

S

-

N

-

G

-

K

-

L

-

I

L

V

P

P

Y

E

Q

D

R

R

D

K

V

I

N

G

T

M

V

V

F

F

Q
|
Q

D

T

Y

W

A

A

L

L

F

Y

P

P

H

N

M

L

N

T

V

A

L

F

D

E

N

N

V

I

A

A

Y

F

G

P

L

L

K

T

V

N

K

M

G

K

V

M

G

S

K

K

T

E

E

E

R

I

Q

R

K

K

R

R

A

V

E

E

A

V

L

A

D

K

M

I

V

L

A

D

L

I

G

H

Y

V

G

L

E

N

R

H

K

F

P

P

V

R

Q

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

L

L

A

A

R

R

A

A

L

L

V

V

N

K

R

D

P

P

R

S

V

L

L

L

D

D

E
|
E

P

P

L

F

G

S

A

N

L

L

D

D

L

A

K

R

L

M

R

R

E

D

Q

S

M

A

Q

R

G

A

E

L

L

V

K

K

K

E

L

V

Q

Q

R

S

Q

R

L

L

G

G

I

V

T

T

F

L

I

L

F

V

V

V

T

S

H

H

D

D

Q

P

T

A

E

D

A

I

L

F

S

A

M

I

S

A

D

D

R

R

V

V

A

G

V

V

F

L

N

V

K

K

G

G

R

K

I

L

E

V

Q

Q

V

V

D

G

T

K

P

P

R

E

N

D

L

L

Y

Y

M

D

K

N

P

P

A

V

T

S