SitesBLAST

Comparing Pf1N1B4_3238 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3238 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

2xuaH Crystal structure of the enol-lactonase from burkholderia xenovorans lb400 (see paper)
49% identity, 96% coverage: 10:266/268 of query aligns to 10:260/261 of 2xuaH

• active site: L32 (= L35), M98 (= M101), D214 (= D220), H241 (= H247)
• binding laevulinic acid: L32 (= L35), W132 (= W135), R135 (= R138), V151 (≠ S157), R154 (= R160), W155 (= W161), Y183 (= Y189), A216 (≠ V222)

6eb3B Structural and enzymatic characterization of an esterase from a metagenomic library
31% identity, 91% coverage: 3:246/268 of query aligns to 3:243/268 of 6eb3B

• binding 2-hydroxypropane-1,3-diyl dibutanoate: S68 (= S74), I69 (= I75), A70 (≠ E76), E102 (≠ W108), H106 (≠ N112), T195 (≠ D198), R197 (≠ D200), Q201 (= Q204)

6eb3A Structural and enzymatic characterization of an esterase from a metagenomic library
31% identity, 91% coverage: 3:246/268 of query aligns to 3:240/265 of 6eb3A

• binding 1,3-dihydroxypropan-2-yl butanoate: I15 (= I15), E16 (≠ Q16), G17 (= G17), Q18 (≠ D25), Q48 (≠ R54)

6eb3C Structural and enzymatic characterization of an esterase from a metagenomic library
31% identity, 91% coverage: 3:246/268 of query aligns to 3:237/262 of 6eb3C

• binding gamma-amino-butanoic acid: G27 (≠ S34), L28 (= L35), S94 (= S100), M95 (= M101)

4uhfA Structural studies of a thermophilic esterase from thermogutta terrifontis (l37a mutant with butyrate bound) (see paper)
30% identity, 75% coverage: 27:226/268 of query aligns to 27:227/278 of 4uhfA

• active site: F34 (≠ L35), L99 (= L99), S100 (= S100), M101 (= M101), D124 (≠ N124), E164 (≠ A164), D221 (= D220)
• binding butanoic acid: G33 (≠ S34), F34 (≠ L35), S100 (= S100)

Sites not aligning to the query:

• active site: 249, 250
• binding butanoic acid: 249, 250

4uheA Structural studies of a thermophilic esterase from thermogutta terrifontis (malate bound) (see paper)
30% identity, 75% coverage: 27:226/268 of query aligns to 27:227/272 of 4uheA

• active site: F34 (≠ L35), L99 (= L99), S100 (= S100), M101 (= M101), D124 (≠ N124), E164 (≠ A164), D221 (= D220)
• binding d-malate: G33 (≠ S34), F34 (≠ L35), S100 (= S100), M101 (= M101), Y104 (≠ L104), R138 (= R138), M196 (≠ V196), I223 (≠ V222)

Sites not aligning to the query:

• active site: 249, 250
• binding d-malate: 249

4uhdA Structural studies of a thermophilic esterase from thermogutta terrifontis (acetate bound) (see paper)
30% identity, 75% coverage: 27:226/268 of query aligns to 27:227/274 of 4uhdA

• active site: F34 (≠ L35), L99 (= L99), S100 (= S100), M101 (= M101), D124 (≠ N124), E164 (≠ A164), D221 (= D220)
• binding acetate ion: G33 (≠ S34), F34 (≠ L35), L99 (= L99), S100 (= S100), M101 (= M101), Y104 (≠ L104), R138 (= R138), M196 (≠ V196)

Sites not aligning to the query:

• active site: 249, 250
• binding acetate ion: 249
• binding magnesium ion: 233, 236, 239

5zhsA Crystal structure of osd14 in complex with covalently bound kk052 (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 17:249/264 of 5zhsA

• binding (4-phenylpiperazin-1-yl)(1H-1,2,3-triazol-1-yl)methanone: G23 (≠ S34), F24 (≠ L35), S93 (= S100), V94 (≠ M101), F122 (≠ I129), F132 (≠ I139), I137 (≠ G146), V140 (≠ A149), Y155 (≠ D165), C187 (= C193), V190 (= V196), F191 (≠ R197), S216 (≠ V222), H243 (= H247)

6ap8A Crystal structure of rice d14 bound to 2-(2-methyl-3-nitroanilino) benzoic acid (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 19:251/266 of 6ap8A

• binding 2-[(2-methyl-3-nitrophenyl)amino]benzoic acid: F26 (≠ L35), S95 (= S100), V96 (≠ M101), F124 (≠ I129), F134 (≠ I139), V142 (≠ A149), W153 (= W161), Y157 (≠ D165), C189 (= C193), V192 (= V196), F193 (≠ R197), S218 (≠ V222), H245 (= H247)

5dj5A Crystal structure of rice dwarf14 in complex with synthetic strigolactone gr24 (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 19:251/266 of 5dj5A

• active site: S95 (= S100)
• binding (3E,3aR,8bS)-3-({[(2R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yl]oxy}methylidene)-3,3a,4,8b-tetrahydro-2H-indeno[1,2-b]furan-2-one: F26 (≠ L35), S95 (= S100), V96 (≠ M101), F124 (≠ I129), F134 (≠ I139), V142 (≠ A149), Y157 (≠ D165), C189 (= C193), V192 (= V196), F193 (≠ R197), V217 (≠ A221), S218 (≠ V222), H245 (= H247)

4ihaA Crystal structure of rice dwarf14 (d14) in complex with a gr24 hydrolysis intermediate (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 21:253/268 of 4ihaA

• active site: S97 (= S100)
• binding (2R,3R)-2,4,4-trihydroxy-3-methylbutanal: G27 (≠ S34), F28 (≠ L35), S97 (= S100), V98 (≠ M101), F126 (≠ I129), V194 (= V196), H247 (= H247)

5zhtA Crystal structure of osd14 in complex with covalently bound kk073 (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 18:250/265 of 5zhtA

• binding (1H-1,2,3-triazol-1-yl){4-[4-(trifluoromethyl)phenyl]piperazin-1-yl}methanone: G24 (≠ S34), F25 (≠ L35), S94 (= S100), V95 (≠ M101), F123 (≠ I129), F133 (≠ I139), V141 (≠ A149), W152 (= W161), Y156 (≠ D165), C188 (= C193), V191 (= V196), F192 (≠ R197), S217 (≠ V222), H244 (= H247)

5zhrA Crystal structure of osd14 in complex with covalently bound kk094 (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 18:250/265 of 5zhrA

• binding (2,3-dihydro-1H-indol-1-yl)(1H-1,2,3-triazol-1-yl)methanone: G24 (≠ S34), F25 (≠ L35), S94 (= S100), V95 (≠ M101), F123 (≠ I129), C188 (= C193), V191 (= V196), F192 (≠ R197), S217 (≠ V222), H244 (= H247)

5yz7A Crystal structure of osd14 in complex with d-ring-opened 7'-carba-4bd (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 18:250/265 of 5yz7A

• binding (2Z,4S)-5-(4-bromophenyl)-4-hydroxy-2-methylpent-2-enoic acid: F25 (≠ L35), S94 (= S100), F133 (≠ I139), V141 (≠ A149), W152 (= W161), Y156 (≠ D165), C188 (= C193), V191 (= V196), F192 (≠ R197), S217 (≠ V222)

Q10QA5 Strigolactone esterase D14; Protein DWARF 14; Protein DWARF 88; Protein HIGH-TILLERING DWARF 2; EC 3.1.-.- from Oryza sativa subsp. japonica (Rice) (see 4 papers)
27% identity, 84% coverage: 28:253/268 of query aligns to 71:303/318 of Q10QA5

• G79 (= G36) mutation to R: In d88; dwarf and high tillering phenotypes.
• S147 (= S100) active site, Nucleophile; binding ; mutation to A: Weakens interaction with D53 and attenuates strigolactone-induced degradation of D53.
• G153 (= G106) mutation to D: In d14; dwarf and high tillering phenotypes.
• F186 (≠ I139) mutation to A: Loss of strigolactone-dependent interaction with SLR1.
• W205 (= W161) mutation to A: Decreased enzymatic activity toward strigolactone and loss of strigolactone-dependent interaction with SLR1.
• C241 (= C193) binding
• F245 (≠ R197) mutation to A: Loss of strigolactone-dependent interaction with SLR1.
• D268 (= D220) active site; mutation to N: Weakens interaction with D53 and attenuates strigolactone-induced degradation of D53.
• H297 (= H247) active site; binding ; mutation to A: No effect on strigolactone binding, but decreased enzymatic activity toward strigolactone and loss of interaction with SLR1.; mutation to Y: Weakens interaction with D53 and attenuates strigolactone-induced degradation of D53.

6brtA F-box protein cth with hydrolase (see paper)
27% identity, 84% coverage: 28:253/268 of query aligns to 38:270/285 of 6brtA

• binding (5R)-5-hydroxy-3-methylfuran-2(5H)-one: F45 (≠ L35), F143 (≠ I129), Y176 (≠ D165), C208 (= C193), V211 (= V196), F212 (≠ R197), S237 (≠ V222)

A0A109QYD3 Strigolactone esterase RMS3; Protein DWARF 14 homolog; PsD14; Protein RAMOSUS 3; EC 3.1.-.- from Pisum sativum (Garden pea) (see paper)
23% identity, 88% coverage: 19:253/268 of query aligns to 12:253/267 of A0A109QYD3

• G15 (= G22) mutation to D: In rms3-3; ramosus phenotype, reduced plant height and extensive vegetative branching from both basal and aerial nodes.
• G28 (= G36) mutation to D: In rms3-4; ramosus phenotype, reduced plant height and extensive vegetative branching from both basal and aerial nodes.
• S96 (= S100) mutation to F: In rms3-5; loss of esterase activity. Ramosus phenotype, reduced plant height and extensive vegetative branching from both basal and aerial nodes.

Q9SQR3 Strigolactone esterase D14; Protein DWARF 14; AtD14; EC 3.1.-.- from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
24% identity, 80% coverage: 28:242/268 of query aligns to 21:240/267 of Q9SQR3

• S97 (= S100) active site, Nucleophile; mutation to A: Loss of activity.
• G158 (≠ R160) mutation to E: Loss of interaction with MAX2.
• P169 (= P171) mutation to L: In d14-seto; loss of activity.

O07015 Sigma factor SigB regulation protein RsbQ from Bacillus subtilis (strain 168) (see paper)
24% identity, 77% coverage: 29:235/268 of query aligns to 21:234/269 of O07015