SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_3430 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3430 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
68% identity, 100% coverage: 1:648/651 of query aligns to 1:648/652 of P27550

query
sites
P27550

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K609 (= K609) modified: N6-acetyllysine; by autocatalysis

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
68% identity, 100% coverage: 1:648/651 of query aligns to 1:648/652 of Q8ZKF6

query
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Q8ZKF6

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R194 (≠ K194) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T311) binding
N335 (= N335) binding
A357 (= A357) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D517) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (= S523) binding
G524 (= G524) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R526) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R584) binding ; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K609) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
70% identity, 96% coverage: 18:643/651 of query aligns to 14:636/640 of 5jrhA

query
sites
5jrhA

L

L

T

I

D

N

E

P

A

E

T

Q

Y

Y

K

E

A

T

M

K

Y

Y

Q

K

Q

Q

S

S

V

I

V

N

N

D

P

P

D

D

G

T

F

F

W

W

R

G

E

E

Q

Q

A

G

K

K

R

I

L

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

T

K

V

V

K

K

Q

N

T

T

S

S

F

F

D

A

D

P

H

G

H

N

V

V

D

S

I

I

K

K

W

W

F

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

E

E

R

N

G

G

D

D

Q

R

I

T

A

A

I

I

W
|
W

E

E

G

G

D

D

P

T

A

S

E

Q

S

S

R
x
K

N

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

C

C

K

R

F

F

A

A

N

N

A

T

L

L

R

L

G

D

Q

L

D

G

V

I

H

K

R

K

G

G

D

D

V

V

T

A

I

I

Y

Y

M

M

P

P

M

M

I
x
V

P

P

E
|
E

A

A

V

A

V

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G
|
G

R
|
R

I

I

D

D

C

S

R

S

S

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

K

R

K

S

I
|
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

A

L

L

T

K

N

N

P

P

E

N

T

V

S

T

S

S

I

V

Q

E

K

H

V

V

I

I

V

V

C
x
L

K

K

R
|
R

T

T

G

G

G

S

D

D

I

I

K

D

W

W

N

Q

Q

E

H

G

R

R

D

D

I

L

W

W

F

W

E

R

D

D

L

L

M

I

K

E

V

K

A

A

G

S

T

P

V

E

C

H

A

Q

P

P

K

E

E

A

M

M

G

N

A

A

E

E

E

D

A

P

L

L

F

F

I

I

L

L

Y
|
Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K
|
K

P
|
P

K

K

G

G

V

V

Q

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

M

T

T

T

H

F

E

K

R

Y

V

V

F

F

D

D

Y

Y

R

H

P

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D
|
D

V

V

G

G

W

W

V

V

T

T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

L

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

I

P

T

A

R

R

V

M

A

C

K

Q

I

V

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

M

L

M

M

A

A

S

E

G

G

T

D

A

K

A

A

V

I

E

E

G

G

A

T

D

D

G

R

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

D

E

W

W

Y

Y

Y

W

K

K

N

K

V

I

G

G

Q

K

S

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

N

F

M

M

M

I

S

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

A

T

R

R

P

P

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

L

E

G

G

N

H

I

P

V

Q

E

E

G

G

E

A

A

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

L

L

F

F

G

G

D

D

H

H

D

E

R

R

F

F

V

E

D

Q

T

T

Y

Y

F

F

K

S

T

T

F

F

R

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G

G

H
|
H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

M

L

V
|
V

A

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

V

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

S

S

E

P

Q

E

L

L

R

Y

L

A

E

E

L

V

K

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

T

A

A

G

E

D

Y

-

D

-

G

-

L

L

G

G

D

D

I

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

Q

E

H

K

L

L

I

L

D

E

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding (r,r)-2,3-butanediol: W93 (= W97), K102 (≠ R106), V138 (≠ I142), E140 (= E144), G169 (= G173), R170 (= R174), L216 (≠ C220), R218 (= R222), Y259 (= Y263), K266 (= K270), P267 (= P271)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G387), E384 (= E388), P385 (= P389), D407 (= D411), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), R511 (= R515), N517 (= N521), R522 (= R526)
binding coenzyme a: F159 (= F163), G160 (= G164), G161 (= G165), R187 (= R191), I192 (= I196), D302 (= D306), S519 (= S523), H521 (= H525), R580 (= R584), P585 (= P589)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (≠ V542)

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
70% identity, 96% coverage: 18:643/651 of query aligns to 14:637/641 of 2p20A

query
sites
2p20A

L

L

T

I

D

N

E

P

A

E

T

Q

Y

Y

K

E

A

T

M

K

Y

Y

Q

K

Q

Q

S

S

V

I

V

N

N

D

P

P

D

D

G

T

F

F

W

W

R

G

E

E

Q

Q

A

G

K

K

R

I

L

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

T

K

V

V

K

K

Q

N

T

T

S

S

F

F

D

A

D

P

H

G

H

N

V

V

D

S

I

I

K

K

W

W

F

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

E

E

R

N

G

G

D

D

Q

R

I

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

A

S

E

Q

S

S

R

K

N

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

C

C

K

R

F

F

A

A

N

N

A

T

L

L

R

L

G

D

Q

L

D

G

V

I

H

K

R

K

G

G

D

D

V

V

T

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F

F

G

G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

D

D

C

S

R

S

S

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R

R

A

A

G

G

K

R

K

S

I

I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

A

L

L

T

K

N

N

P

P

E

N

T

V

S

T

S

S

I

V

Q

E

K

H

V

V

I

I

V

V

C

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

K

D

W

W

N

Q

Q

E

H

G

R

R

D

D

I

L

W

W

F

W

E

R

D

D

L

L

M

I

K

E

V

K

A

A

G

S

T

P

V

E

C

H

A

Q

P

P

K

E

E

A

M

M

G

N

A

A

E

E

E

D

A

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

Q

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

M

T

T

T

H

F

E

K

R

Y

V

V

F

F

D

D

Y

Y

R

H

P

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A

A

D

D

V

V

G

G

W

W

V
|
V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

L

M

F

F

E

E

G

G

V

V

P

P

N

N

Y

W

P

P

D

T

I

P

T

A

R

R

V

M

A

C

K

Q

I

V

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A

A

P

P

T

T

A

A

I

I

R

R

A

A

M

L

M

M

A

A

S

E

G

G

T

D

A

K

A

A

V

I

E

E

G

G

A

T

D

D

G

R

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

D

E

W

W

Y

Y

Y

W

K

K

N

K

V

I

G

G

Q

K

S

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

N

F

M

M

M

I

S

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

A

T

R

R

P

P

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

L

E

G

G

N

H

I

P

V

Q

E

E

G

G

E

A

A

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

L

L

F

F

G

G

D

D

H

H

D

E

R

R

F

F

V

E

D

Q

T

T

Y

Y

F

F

K

S

T

T

F

F

R

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S

S

G

G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

M

L

V

V

A

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

V

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

S

S

E

P

Q

E

L

L

R

Y

L

A

E

E

L

V

K

R

N

N

W

W

V

V

R

A

K

K

E

E

I

I

G

G

P

P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

T

A

A

G

E

-

Y

-

D

D

G

T

L

S

G

N

D

D

I

T

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

Q

E

H

K

L

L

I

L

D

E

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding adenosine-5'-monophosphate-propyl ester: V306 (= V310), T307 (= T311), G383 (= G387), E384 (= E388), P385 (= P389), D407 (= D411), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), R511 (= R515), N517 (= N521), R522 (= R526)

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
70% identity, 96% coverage: 18:643/651 of query aligns to 13:633/637 of 2p2fA

query
sites
2p2fA

L

L

T

I

D

N

E

P

A

E

T

Q

Y

Y

K

E

A

T

M

K

Y

Y

Q

K

Q

Q

S

S

V

I

V

N

N

D

P

P

D

D

G

T

F

F

W

W

R

G

E

E

Q

Q

A

G

K

K

R

I

L

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

T

K

V

V

K

K

Q

N

T

T

S

S

F

F

D

A

D

P

H

G

H

N

V

V

D

S

I

I

K

K

W

W

F

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

E

E

R

N

G

G

D

D

Q

R

I

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

A

S

E

Q

S

S

R

K

N

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

C

C

K

R

F

F

A

A

N

N

A

T

L

L

R

L

G

D

Q

L

D

G

V

I

H

K

R

K

G

G

D

D

V

V

T

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

R

I

I

D

D

C

S

R

S

S

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

K

R

K

S

I
|
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

A

L

L

T

K

N

N

P

P

E

N

T

V

S

T

S

S

I

V

Q

E

K

H

V

V

I

I

V

V

C

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

K

D

W

W

N

Q

Q

E

H

G

R

R

D

D

I

L

W

W

F

W

E

R

D

D

L

L

M

I

K

E

V

K

A

A

G

S

T

P

V

E

C

H

A

Q

P

P

K

E

E

A

M

M

G

N

A

A

E

E

E

D

A

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

Q

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

M

T

T

T

H

F

E

K

R

Y

V

V

F

F

D

D

Y

Y

R

H

P

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W
|
W

V

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

L

M

F

F

E

E

G

G

V
|
V

P
|
P

N

N

Y

W

P

P

D

T

I

P

T

A

R

R

V

M

A

C

K

Q

I

V

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

A

A

M

L

M

M

A

A

S

E

G

G

T

D

A

K

A

A

V

I

E

E

G

G

A

T

D

D

G

R

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

D

E

W

W

Y

Y

Y

W

K

K

N

K

V

I

G

G

Q

K

S

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

N

F

M

M

M

I

S

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

A

T

R

R

P

P

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

L

E

G

G

N

H

I

P

V

Q

E

E

G

G

E

A

A

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

L

L

F

F

G

G

D

D

H

H

D

E

R

R

F

F

V

E

D

Q

T

T

Y

Y

F

F

K

S

T

T

F

F

R

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G
|
G

H

H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

M

L

V

V

A

A

H

H

P

P

K

K

V

I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

V

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

S

S

E

P

Q

E

L

L

R

Y

L

A

E

E

L

V

K

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

T

A

A

G

E

D

Y

T

D

S

G

-

L

-

G

-

D

-

I

-

S

S

T

T

L

L

A

A

D

D

P

P

G

G

V

V

Q

E

H

K

L

L

I

L

D

E

active site: T259 (= T264), T411 (= T416), E412 (= E417), N516 (= N521), R521 (= R526), K604 (= K609)
binding adenosine monophosphate: G382 (= G387), E383 (= E388), P384 (= P389), D406 (= D411), T407 (= T412), W408 (= W413), W409 (= W414), Q410 (= Q415), T411 (= T416), D495 (= D500), I507 (= I512), R510 (= R515), N516 (= N521), R521 (= R526)
binding coenzyme a: F158 (= F163), G159 (= G164), G160 (= G165), R186 (= R191), I191 (= I196), A300 (= A305), W304 (= W309), T306 (= T311), V328 (= V333), P329 (= P334), A352 (= A357), T354 (= T359), A355 (= A360), S518 (= S523), G519 (= G524), R579 (= R584), P584 (= P589)

1pg3A Acetyl coa synthetase, acetylated on lys609 (see paper)
69% identity, 96% coverage: 18:643/651 of query aligns to 14:630/634 of 1pg3A

query
sites
1pg3A

L

L

T

I

D

N

E

P

A

E

T

Q

Y

Y

K

E

A

T

M

K

Y

Y

Q

K

Q

Q

S

S

V

I

V

N

N

D

P

P

D

D

G

T

F

F

W

W

R

G

E

E

Q

Q

A

G

K

K

R

I

L

L

D

D

W

W

I

I

K

T

P

P

F

Y

T

Q

T

K

V

V

K

K

Q

N

T

T

S

S

F

F

D

A

D

P

H

G

H

N

V

V

D

S

I

I

K

K

W

W

F

Y

A

E

D

D

G

G

T

T

L

L

N

N

V

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

L

L

A

Q

E

E

R

N

G

G

D

D

Q

R

I

T

A

A

I

I

W

W

E

E

G

G

D

D

P

T

A

S

E

Q

S

S

R

K

N

H

I

I

T

S

Y

Y

R

R

E

E

L

L

H

H

E

R

Q

D

V

V

C

C

K

R

F

F

A

A

N

N

A

T

L

L

R

L

G

D

Q

L

D

G

V

I

H

K

R

K

G

G

D

D

V

V

T

A

I

I

Y

Y

M

M

P

P

M

M

I

V

P

P

E

E

A

A

V

A

V

V

A

A

M

M

L

L

A

A

C

C

T

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

G
|
G

F

F

S

S

P

P

E

E

A

A

L

V

A

A

G

G

R

C

I

I

D

D

C

S

R

S

S

S

K

R

V

L

V

V

I

I

T

T

A

A

D

D

E

E

G

G

V

V

R
|
R

A

A

G

G

K
x
R

K

S

I
|
I

P

P

L

L

K

K

A

K

N

N

V

V

D

D

A

A

L

L

T

K

N

N

P

P

E

N

T

V

S

T

S

S

I

V

Q

E

K

H

V

V

I

I

V

V

C

L

K

K

R

R

T

T

G

G

G

S

D

D

I

I

K

D

W

W

N

Q

Q

E

H

G

R

R

D

D

I

L

W

W

F

W

E

R

D

D

L

L

M

I

K

E

V

K

A

A

G

S

T

P

V

E

C

H

A

Q

P

P

K

E

E

A

M

M

G

N

A

A

E

E

E

D

A

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

S

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

Q

L

H

H

T

T

G

G

Y

Y

L

L

L

V

Y

Y

A

A

M

T

T

T

H

F

E

K

R

Y

V

V

F

F

D

D

Y

Y

R

H

P

P

G

G

E

D

I

I

Y

Y

W

W

C

C

T

T

A
|
A

D

D

V

V

G

G

W

W

V
|
V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

L

L

L

M

F

F

E

E

G

G

V
|
V

P
|
P

N
|
N

Y

W

P

P

D

T

I

P

T

A

R

R

V

M

A

C

K

Q

I

V

V

V

D

D

K

K

H

H

K

Q

V

V

N

N

I

I

L

L

Y

Y

T

T

A
|
A

P

P

T
|
T

A
|
A

I

I

R

R

A

A

M

L

M

M

A

A

S

E

G

G

T

D

A

K

A

A

V

I

E

E

G

G

A

T

D

D

G

R

S

S

L

L

R

R

L

I

L

L

G

G

S

S

V
|
V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

D

E

W

W

Y

Y

Y

W

K

K

N

K

V

I

G

G

Q

K

S

E

R

K

C

C

P

P

I

V

V

V

D
|
D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

N

F

M

M

M

I

S

T

P

P

L

L

P

P

G

G

A

A

H

I

A

E

L

L

K

K

P

A

G

G

S

S

A

A

A

T

R

R

P

P

F

F

G

G

V

V

V

Q

P

P

A

A

L

L

V

V

D

D

N

N

L

E

G

G

N

H

I

P

V

Q

E

E

G

G

E

A

A

T

E

E

G

G

N

N

L

L

V

V

I

I

L

T

D

D

S

S

W

W

P

P

G

G

Q

Q

A

A

R

R

T

T

L

L

F

F

G

G

D

D

H

H

D

E

R

R

F

F

V

E

D

Q

T

T

Y

Y

F

F

K

S

T

T

F

F

R

K

G

N

M

M

Y

Y

F

F

T

S

G

G

D
|
D

G

G

A

A

R

R

D

D

A

E

D

D

G

G

Y

Y

W

W

I
|
I

T

T

G

G

R
|
R

V

V

D

D

V

V

L

L

N
|
N

V

V

S
|
S

G
|
G

H
|
H

R
|
R

M

L

G

G

T

T

A

A

E

E

I

I

E

E

S

S

A

A

M

L

V
|
V

A

A

H
|
H

P

P

K

K

V
x
I

A

A

E

E

A

A

V

V

G

G

V

I

P

P

H

H

D

A

I

I

K

K

G

G

Q

Q

G

A

I

I

Y

Y

V

A

Y

Y

V

V

T

T

L

L

N

N

A

H

G

G

E

E

P

P

S

S

E

P

Q

E

L

L

R

Y

L

A

E

E

L

V

K

R

N

N

W

W

V

V

R
|
R

K

K

E

E

I

I

G

G

P
|
P

I

L

A

A

S

T

P

P

D

D

V

V

I

L

Q

H

W

W

A

T

P

D

G

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

I

I

L

L

R

R

K

K

I

I

A

A

T

A

A

G

E

D

Y

-

D

-

G

-

L

-

G

-

D

-

I

-

S

-

T

-

L

L

A

A

D

D

P

P

G

G

V

V

Q

E

H

K

L

L

I

L

D

E

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N521), R522 (= R526), K605 (= K609)
binding coenzyme a: F159 (= F163), G160 (= G164), G161 (= G165), R187 (= R191), R190 (≠ K194), I192 (= I196), A301 (= A305), T307 (= T311), V329 (= V333), P330 (= P334), N331 (= N335), A353 (= A357), T355 (= T359), A356 (= A360), S519 (= S523), G520 (= G524), H521 (= H525), R580 (= R584), P585 (= P589)
binding magnesium ion: V533 (= V537), H535 (= H539), I538 (≠ V542)
binding adenosine-5'-monophosphate-propyl ester: V306 (= V310), T307 (= T311), V382 (= V386), G383 (= G387), E384 (= E388), P385 (= P389), D407 (= D411), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D500), I508 (= I512), R511 (= R515), R522 (= R526)

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
66% identity, 100% coverage: 3:651/651 of query aligns to 2:648/648 of Q89WV5

query
sites
Q89WV5

A

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