SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_3487 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3487 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
29% identity, 91% coverage: 4:409/444 of query aligns to 9:421/425 of O59010

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O59010

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S65 (≠ T58) mutation to V: Strongly decreased chloride conductance.
R276 (≠ S269) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ SSS 269:271) binding
M311 (≠ L304) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (= T307) binding
V355 (= V348) binding
D394 (≠ S382) binding
M395 (≠ E383) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R385) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N389) binding
D405 (≠ N393) mutation to N: Strongly decreased affinity for aspartate.

2nwwA Crystal structure of gltph in complex with tboa (see paper)
29% identity, 89% coverage: 6:402/444 of query aligns to 2:405/407 of 2nwwA

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binding (3s)-3-(benzyloxy)-l-aspartic acid: R267 (≠ S269), S268 (= S270), S269 (= S271), T305 (= T307), G348 (= G350), A349 (≠ S351), G350 (= G352), D385 (≠ S382), R388 (= R385), T389 (≠ A386), N392 (= N389)

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
28% identity, 90% coverage: 4:402/444 of query aligns to 9:414/419 of 6x15A

query
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A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L
x
M

T

V

N
|
N

L
x
V

V

T

G

G

N
x
D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

K

K

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: K15 (≠ S10), I18 (≠ F13), Y42 (vs. gap), P45 (= P38), F46 (≠ L39), F46 (≠ L39), F46 (≠ L39), L49 (≠ G42), L49 (≠ G42), L53 (= L46), I73 (≠ M66), P75 (≠ N68), K84 (≠ Y78), L90 (≠ E84), L91 (≠ I85), L91 (≠ I85), F95 (≠ I89), L130 (≠ I131), V131 (≠ I132), I133 (≠ F134), L134 (≠ I135), I137 (≠ V138), L152 (= L153), F156 (≠ M157), I159 (≠ V160), I163 (≠ F164), I163 (≠ F164), I163 (≠ F164), A164 (= A165), Y167 (vs. gap), Y167 (vs. gap), K196 (≠ N189), I197 (= I190), N199 (= N192), G200 (≠ M193), Y204 (≠ L197), I207 (≠ V200), F210 (= F203), L250 (≠ I243), I253 (≠ A246), Y254 (≠ H247), Y254 (≠ H247), I262 (= I255), K266 (≠ R259), M269 (≠ L262), T334 (≠ D327), V335 (≠ L328), G336 (≠ T329), Q337 (≠ A330), L339 (≠ I332), T340 (= T333), L343 (= L336), S369 (≠ A362), M399 (≠ L387)
binding aspartic acid: R276 (≠ S269), S277 (= S270), S278 (= S271), T314 (= T307), T352 (≠ A345), G354 (= G347), V355 (= V348), G357 (= G350), A358 (≠ S351), G359 (= G352), D394 (≠ S382), R397 (= R385), T398 (≠ A386), N401 (= N389)
binding sodium ion: Y89 (≠ F83), T92 (≠ V86), S93 (= S87), G306 (= G299), A307 (≠ Y300), T308 (≠ S301), N310 (= N303), N310 (= N303), M311 (≠ L304), D312 (= D305), S349 (= S342), I350 (≠ K343), G351 (= G344), T352 (≠ A345), N401 (= N389), V402 (≠ L390), D405 (≠ N393)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 3, 4, 4, 6, 7, 8

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
28% identity, 90% coverage: 4:402/444 of query aligns to 6:411/413 of 6x14A

query
sites
6x14A

R

E

Q

Y

P

P

L

V

Y

L

K

Q

S

K

L
x
I

Y

L

F

I

Q

G

V

L

I

I

V

L

A

G

I

A

A

I

I

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

Y

G

P

Y

Q

A

T

H

G

A

V

V

A

H

-

T

-

Y

L

V

K

K

P

P

L

F

G

G

D

D

G

L

F
|
F

I

V

K

R

L

L

I

L

K

C

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

F

C

A

T

S

V

L

V

V

S

V

G
|
G

I
x
A

A

A

G

S

M
x
I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L
x
V

L

V

Y

Y

F

Y

E

L

I

L

V

T

S

S

T

A

I

F

A

A

L

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L

T

I

L

G

G

L

I

V

I

V

M

V

A

N

R

V

L

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F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

V

V

T

G

L

Q

D

Q

T

-

S

-

K

-

I

-

A

-

G

-

F

-

I

F

S

Q

A

P

G

H

K

Q

D

A

Q

P

S

P

I

L

I

V

G

H

F

I

I

L

L

L

N

D

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

N

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

M

F

F

F

S

A

V

I

I
|
I

F

L

G

G

F

I

A

A

L

I

-

T

-
x
Y

-

L

-

M

-

N

-

S

-

E

-

N

H

E

R

K

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

D

F

A

I

I

D

N

R

G

F

L

A

A

H

E

V

A

M

M

F

Y

N
x
K

I
|
I

I

V

N
|
N

M
x
G

I

V

M
|
M

K
x
Q

L
x
Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

H

S

V

L

V

V

G

Q

E

L

L

G

A

Q

K

L

V

M

T

I

A

C

A

F

V

Y

Y

I

V

T

G

C

L

I

T

L

L

F

Q

V

I

V

L

L

L

V

V

L

Y

G

F

A

V

I

L

C

L

R

K

A

I

H

Y

G

G

F

I

S

D

V

P

I

I

K

S

L

F

I

I

R

K

Y

H

I

A

R

K

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D

E

A

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x
R

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|
S

E

S

S

G

A

T

L

L

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P

R

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T

L

M

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R

K

V

M

A

E

K

R

E

L

M

G

G

A

I

Q

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K

E

S

G

V

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V

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G

S

L

F

V

T

I

L

P

P

T
x
L

G

G

Y

A

S
x
T

F
x
I

N

N

L
x
M

D

D

G

G

T
|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

A

A

T

V

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

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T

S

P

H

M

L

D

T

L

V

T

G

A

Q

Q

Q

I

L

T

T

L

I

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S

S

K
x
I

G

G

A

T

A
|
A

G

G

V

V

T
x
P

G

G

S

A

G
|
G

F
x
A

I

I

V

M

L

L

A

C

A

M

T

V

L

L

S

H

A

S

V

V

G

G

S

-

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

A

A

G

A

L

Y

A

A

L

M

I

I

L
|
L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

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A

G

T

T

L

A

V

I

V

V

A

A

K

K

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G62), A67 (≠ I63), I70 (≠ M66), V83 (≠ L80), I157 (= I161), Y164 (vs. gap), K193 (≠ N189), I194 (= I190), N196 (= N192), G197 (≠ M193), Q200 (≠ K196), Y201 (≠ L197), L302 (≠ T298), T305 (≠ S301), I306 (≠ F302), I347 (≠ K343), A350 (= A346)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ L11), F47 (= F43), M199 (= M195), R273 (≠ S269), S274 (= S270), S275 (= S271), M308 (≠ L304), T311 (= T307), P353 (≠ T349), G356 (= G352), A357 (≠ F353), L384 (= L375), D391 (≠ S382), R394 (= R385), T395 (≠ A386)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 1, 4

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
28% identity, 90% coverage: 4:402/444 of query aligns to 1:406/408 of 6bauA

query
sites
6bauA

R

E

Q

Y

P

P

L

V

Y

L

K

Q

S

K

L

I

Y

L

F

I

Q

G

V

L

I

I

V

L

A

G

I

A

A

I

I

V

G

G

I

L

L

I

L

L

G

G

H

H

F

Y

Y

G

P

Y

Q

A

T

D

G

A

V

V

A

K

-

T

-

Y

L

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K

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P

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F

G

G

D

D

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F

I

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R

L

L

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L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

F

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

L

V

T

S

S

T

A

I

F

A

A

L

V

L

T

I

L

G

G

L

I

V

I

V

M

V

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

V

V

T

G

L

Q

D

Q

T

-

S

-

K

-

I

-

A

-

G

-

F

-

I

F

S

Q

A

P

G

K

K

Q

D

A

Q

P

S

P

I

L

I

V

G

K

F

I

I

L

L

L

N

D

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

N

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

-

M

-

N

-

S

-

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-

N

H

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G

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A

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Y

S

G

A

K

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L

L

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D

F

A

I

I

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N

R

G

F

L

A

A

H

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A

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M

F

Y

N

K

I

I

I

V

N

N

M

G

I

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M

M

K

Q

L

Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

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S

V

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L

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A

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I

A

C

A

F

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Y

Y

I

V

T

G

C

L

I

T

L

L

F

Q

V

I

V

L

L

L

V

V

L

Y

G

F

A

V

I

L

C

L

R

K

A

I

H

Y

G

G

F

I

S

D

V

P

I

I

K

S

L

F

I

I

R

K

Y

K

I

A

R

K

E

D

E

A

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M

L

L

I

T

V

A

L

F

G

V

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T

S
x
R

S

S

S
|
S

E

S

S

G

A

T

L

L

P

P

R

V

M

T

L

M

I

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K

V

M

A

E

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R

E

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G

G

A

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E

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G

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I

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Y

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F

V

T

I

L

P

P

T

L

G

G

Y

A

S

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L
x
M

D

D

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|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

C

A

T

V

F

F

F

I

I

A

A

Q

N

A

A

T

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D

G

T

S

P

H

M

L

D

T

L

V

T

G

A

Q

Q

Q

I

L

T

T

L

I

L

V

L

L

V

T

L

A

L

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A

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G

G

A

T

A
|
A

G
|
G

V
|
V

T
x
P

G

G

S

A

G
|
G

F

A

I

I

V

M

L

L

A

A

A

M

T

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L

L

S

E

A

S

V

V

G

G

S

-

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

A

A

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
x
C

A
x
T

L

M

T

V

N
|
N

L

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V

T

G

G

N

D

A

L

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A

G

T

T

L

A

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I

V

V

A

A

K

K

binding cysteine: R268 (≠ S269), S270 (= S271), M303 (≠ L304), T306 (= T307), A345 (= A346), G346 (= G347), V347 (= V348), P348 (≠ T349), G351 (= G352), D386 (≠ S382), C389 (≠ R385), T390 (≠ A386), N393 (= N389)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
28% identity, 90% coverage: 4:402/444 of query aligns to 1:406/409 of 6bavA

query
sites
6bavA

R

E

Q

Y

P

P

L

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Y

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K

Q

S

K

L

I

Y

L

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L

I

I

V

L

A

G

I

A

A

I

I

V

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G

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L

I

L

L

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G

H

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F

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Q

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G

A

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A

K

-

T

-

Y

L

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K

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P

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G

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D

G

L

F

F

I

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R

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L

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L

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I

I

I

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F

F

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

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Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

L

V

T

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S

T

A

I

F

A

A

L

V

L

T

I

L

G

G

L

I

V

I

V

M

V

A

N

R

V

L

V

F

Q

N

P

P

G

G

A

A

G

G

M

I

H

H

I

L

D

A

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G

L

Q

D

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K

-

I

-

A

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-

I

F

S

Q

A

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K

K

Q

D

A

Q

P

S

P

I

L

I

V

G

K

F

I

I

L

L

L

N

D

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

G

A

A

F

L

A

A

N

N

G

G

D

Q

I

V

L

L

Q

P

V

T

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

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M

-

N

-

S

-

E

-

N

H

E

R

K

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

D

F

A

I

I

D

N

R

G

F

L

A

A

H

E

V

A

M

M

F

Y

N

K

I

I

I

V

N

N

M

G

I

V

M

M

K

Q

L

Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

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S

V

L

V

V

G

Q

E

L

L

G

A

Q

K

L

V

M

T

I

A

C

A

F

V

Y

Y

I

V

T

G

C

L

I

T

L

L

F

Q

V

I

V

L

L

L

V

V

L

Y

G

F

A

V

I

L

C

L

R

K

A

I

H

Y

G

G

F

I

S

D

V

P

I

I

K

S

L

F

I

I

R

K

Y

K

I

A

R

K

E

D

E

A

L

M

L

L

I

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

A

T

L

L

P

P

R

V

M

T

L

M

I

R

K

V

M

A

E

K

R

E

L

M

G

G

A

I

Q

S

K

E

S

G

V

I

V

Y

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

S

A

I

L

Y
|
Y

L

Q

T

G

M

V

A

C

A

T

V

F

F

F

I

I

A

A

Q

N

A

A

T

L

D

G

T

S

P

H

M

L

D

T

L

V

T

G

A

Q

Q

Q

I

L

T

T

L

I

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

V

V

T

P

G

G

S

A

G

G

F

A

I

I

V

M

L

L

A

A

A

M

T

V

L

L

S

E

A

S

V

V

G

G

S

-

L

L

P

P

V

L

-

T

-

D

-

P

-

N

-

V

-

A

A

A

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D
|
D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
x
C

A
x
T

L

M

T

V

N
|
N

L

V

V

T

G

G

N

D

A

L

V

T

A

G

T

T

L

A

V

I

V

V

A

A

K

K

binding benzylcysteine: R268 (≠ S269), S269 (= S270), S270 (= S271), M303 (≠ L304), T306 (= T307), Y309 (= Y310), D382 (= D378), D386 (≠ S382), C389 (≠ R385), T390 (≠ A386), N393 (= N389)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
30% identity, 91% coverage: 7:410/444 of query aligns to 8:419/424 of 6zl4A

query
sites
6zl4A

L

L

Y

W

K

K

S

I

L

L

Y

W

F

G

Q

L

V

V

I

L

V

G

A

A

I

V

A

-

I

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

Y

G

P

Y

Q

A

T

G

G

A

V

V

A

K

-

T

-

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

L

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

A

V

T

S

S

T

A

I

M

A

A

L

V

L

F

I

F

G

G

L

L

V

I

V

V

V

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

M

V

H

N

I

L

D

G

V

S

T

G

T

T

L

-

D

-

T

-

S

-

K

-

I

-

A

-

G

G

F

K

I

A

S

I

A

E

G

A

K

Q

D

P

Q

P

S

S

I

L

I

V

G

Q

F

T

I

L

L

L

N

N

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

N

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

H

E

R

R

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

R

F

V

I

F

D

D

R

G

F

L

A

A

H

E

V

A

M

M

F
x
Y

N
x
L

I

I

I

V

N
x
G

M
x
G

I

V

M

M

K
x
Q

L

Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

R

S

V

L

V

V

G

Q

P

L

L

G

A

Q

K

L

V

M

V

I

G

C

A

F

V

Y

Y

I

T

T

G

C

L

I

F

L

L

F

Q

V

I

V

V

L

I

V

T

L

Y

G

F

A

I

I

L

C

L

R

K

A

V

H

F

G

G

F

I

S

D

V

P

I

I

K

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

A

T

L

L

P

P

-

V

R

T

M

M

L
x
R

I

V

K

A

M

E

E
|
E

R

E

L

M

G

G

A

V

Q

D

K

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

T

A

V

V

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

T

H

P

P

M

L

D

T

L

L

T

G

A

Q

Q

Q

I

L

T

V

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

V
|
V

T
x
P

G
|
G

S
x
A

G
|
G

F

A

I

I

V

M

L

L

A

A

A

M

T

V

L

L

S

Q

A

S

V

V

G

G

-

L

-

D

-

L

-

T

-

P

S

G

L

S

P

P

V

V

A

A

-

L

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

K

K

W

T

V

E

K

K

E

E

L

L

D

D

E

E

D

S

binding decyl-beta-d-maltopyranoside: Y190 (≠ F188), L191 (≠ N189), G194 (≠ N192), G195 (≠ M193), Q198 (≠ K196), R282 (≠ L279), E286 (= E283)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S269), S272 (= S270), S273 (= S271), M307 (≠ L304), T310 (= T307), V351 (= V348), P352 (≠ T349), G353 (= G350), A354 (≠ S351), G355 (= G352), D391 (≠ S382), R394 (= R385), T395 (≠ A386), N398 (= N389)

Sites not aligning to the query:

binding decyl-beta-d-maltopyranoside: 3

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
30% identity, 91% coverage: 7:410/444 of query aligns to 9:420/425 of 6zgbA

query
sites
6zgbA

L

L

Y

W

K

K

S

I

L

L

Y

W

F

G

Q

L

V

V

I

L

V

G

A

A

I

V

A

-

I

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

Y

G

P

Y

Q

A

T

G

G

A

V

V

A

K

-

T

-

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

L

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

A

V

T

S

S

T

A

I

M

A

A

L

V

L

F

I

F

G

G

L

L

V

I

V

V

V

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

M

V

H

N

I

L

D

G

V

S

T

G

T

T

L

-

D

-

T

-

S

-

K

-

I

-

A

-

G

G

F

K

I

A

S

I

A

E

G

A

K

Q

D

P

Q

P

S

S

I

L

I

V

G

Q

F

T

I

L

L

L

N

N

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

N

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

H

E

R

R

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

R

F

V

I

F

D

D

R

G

F

L

A

A

H

E

V

A

M

M

F

Y

N

L

I

I

I

V

N

G

M

G

I

V

M

M

K

Q

L

Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

R

S

V

L

V

V

G

Q

P

L

L

G

A

Q

K

L

V

M

V

I

G

C

A

F

V

Y

Y

I

T

T

G

C

L

I

F

L

L

F

Q

V

I

V

V

L

I

V

T

L

Y

G

F

A

I

I

L

C

L

R

K

A

V

H

F

G

G

F

I

S

D

V

P

I

I

K

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

A

T

L

L

P

P

-

V

R

T

M

M

L

R

I

V

K

A

M

E

E

E

R

E

L

M

G

G

A

V

Q

D

K

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

T

A

V

V

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

T

H

P

P

M

L

D

T

L

L

T

G

A

Q

Q

Q

I

L

T

V

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

V

V

T

P

G

G

S

A

G

G

F

A

I

I

V

M

L

L

A

A

A

M

T

V

L

L

S

Q

A

S

V

V

G

G

-

L

-

D

-

L

-

T

-

P

S

G

L

S

P

P

V

V

A

A

-

L

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

K

K

W

T

V

E

K

K

E

E

L

L

D

D

E

E

D

S

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ S269), S273 (= S270), S274 (= S271), M308 (≠ L304), T311 (= T307), D392 (≠ S382), R395 (= R385), T396 (≠ A386)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
30% identity, 91% coverage: 7:410/444 of query aligns to 11:422/427 of 5e9sA

query
sites
5e9sA

L

L

Y

W

K

K

S

I

L

L

Y

W

F

G

Q

L

V

V

I

L

V

G

A

A

I

V

A

-

I

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

Y

G

P

Y

Q

A

T

G

G

A

V

V

A

K

-

T

-

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

L

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F
x
Y

E

L

I

A

V
x
T

S
|
S

T

A

I

M

A

A

L

V

L

F

I

F

G

G

L

L

V

I

V

V

V

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

M

V

H

N

I

L

D

G

V

S

T

G

T

T

L

-

D

-

T

-

S

-

K

-

I

-

A

-

G

G

F

K

I

A

S

I

A

E

G

A

K

Q

D

P

Q

P

S

S

I

L

I

V

G

Q

F

T

I

L

L

L

N

N

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

N

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

H

E

R

R

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

R

F

V

I

F

D

D

R

G

F

L

A

A

H

E

V

A

M

M

F

Y

N
x
L

I

I

I

V

N
x
G

M
x
G

I

V

M

M

K

Q

L
x
Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

R

S

V

L

V

V

G

Q

P

L

L

G

A

Q

K

L

V

M

V

I

G

C

A

F

V

Y

Y

I

T

T

G

C

L

I

F

L

L

F

Q

V

I

V

V

L

I

V

T

L

Y

G

F

A

I

I

L

C

L

R

K

A

V

H

F

G

G

F

I

S

D

V

P

I

I

K

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

A

T

L

L

P

P

-

V

R

T

M

M

L
x
R

I

V

K

A

M

E

E

E

R

E

L

M

G

G

A

V

Q

D

K

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G
|
G

Y
x
A

S
x
T

F
x
I

N
|
N

L
x
M

D
|
D

G

G

T
|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

T

A

V

V

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

T

H

P

P

M

L

D

T

L

L

T

G

A

Q

Q

Q

I

L

T

V

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S
|
S

K
x
I

G
|
G

A
x
T

A
|
A

G
|
G

V
|
V

T
x
P

G
|
G

S
x
A

G
|
G

F

A

I

I

V

M

L

L

A

A

A

M

T

V

L

L

S

Q

A

S

V

V

G

G

-

L

-

D

-

L

-

T

-

P

S

G

L

S

P

P

V

V

A

A

-

L

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L

M

T

V

N
|
N

L
x
V

V

T

G

G

N
x
D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

K

K

W

T

V

E

K

K

E

E

L

L

D

D

E

E

D

S

binding aspartic acid: R274 (≠ S269), S275 (= S270), S276 (= S271), T313 (= T307), T351 (≠ A345), A352 (= A346), G353 (= G347), V354 (= V348), P355 (≠ T349), G356 (= G350), A357 (≠ S351), G358 (= G352), D394 (≠ S382), R397 (= R385), T398 (≠ A386), N401 (= N389)
binding decyl-beta-d-maltopyranoside: L194 (≠ N189), G197 (≠ N192), G198 (≠ M193), Y202 (≠ L197), R285 (≠ L279)
binding sodium ion: Y87 (≠ F83), T90 (≠ V86), S91 (= S87), S276 (= S271), G305 (= G299), A306 (≠ Y300), T307 (≠ S301), I308 (≠ F302), N309 (= N303), N309 (= N303), M310 (≠ L304), M310 (≠ L304), D311 (= D305), S348 (= S342), I349 (≠ K343), G350 (= G344), T351 (≠ A345), N401 (= N389), V402 (≠ L390), D405 (≠ N393)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
30% identity, 91% coverage: 7:410/444 of query aligns to 11:422/426 of 6xwnB

query
sites
6xwnB

L

L

Y

W

K

K

S

I

L

L

Y

W

F

G

Q

L

V

V

I

L

V

G

A

A

I

V

A

-

I

F

G

G

I

L

L

I

L

A

G

G

H

H

F

F

Y

G

P

Y

Q

A

T

G

G

A

V

V

A

K

-

T

-

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

L

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

A

V

T

S

S

T

A

I

M

A

A

L

V

L

F

I

F

G

G

L

L

V

I

V

V

V

G

N

R

V

L

V

F

Q

N

P

V

G

G

A

A

G

N

M

V

H

N

I

L

D

G

V

S

T

G

T

T

L

-

D

-

T

-

S

-

K

-

I

-

A

-

G

G

F

K

I

A

S

I

A

E

G

A

K

Q

D

P

Q

P

S

S

I

L

I

V

G

Q

F

T

I

L

L

L

N

N

V

I

I

V

P

P

N

T

T

N

I

P

V

F

G

A

A

S

F

L

A

A

N

K

G

G

D

E

I

V

L

L

Q

P

V

V

L

I

M

F

F

F

S

A

V

I

I

I

F

L

G

G

F

I

A

A

L

I

-

T

-

Y

-

L

-

M

-

N

-

R

-

N

-

E

H

E

R

R

L

V

G

R

A

K

Y

S

G

A

K

E

P

T

V

L

L

L

D

R

F

V

I

F

D

D

R

G

F

L

A

A

H

E

V

A

M

M

F

Y

N

L

I

I

I

V

N

G

M

G

I

V

M

M

K

Q

L

Y

A

A

P

P

V

I

G

G

A

V

F

F

G

A

A

L

M

I

A

A

F

Y

T

V

I

M

G

A

A

E

Y

Q

G

G

V

V

G

R

S

V

L

V

V

G

Q

P

L

L

G

A

Q

K

L

V

M

V

I

G

C

A

F

V

Y

Y

I

T

T

G

C

L

I

F

L

L

F

Q

V

I

V

V

L

I

V

T

L

Y

G

F

A

I

I

L

C

L

R

K

A

V

H

F

G

G

F

I

S

D

V

P

I

I

K

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

E

A

L

M

L

I

I

T

V

A

L

F

G

V

T

T

S
x
R

S
|
S

E

S

S

G

A

T

L

L

P

P

-

V

R

T

M

M

L

R

I

V

K

A

M

E

E

E

R

E

L

M

G

G

A

V

Q

D

K

K

S

G

V

I

V

F

G

S

L

F

V

T

I

L

P

P

T

L

G

G

Y

A

S
x
T

F

I

N

N

L
x
M

D

D

G

G

T
|
T

S

A

I

L

Y

Y

L

Q

T

G

M

V

A

T

A

V

V

L

F

F

I

V

A

A

Q

N

A

A

T

I

D

G

T

H

P

P

M

L

D

T

L

L

T

G

A

Q

Q

Q

I

L

T

V

L

V

L

L

V

T

L

A

L

V

L

L

S

A

S

S

K

I

G

G

A

T

A

A

G

G

V

V

T

P

G

G

S

A

G
|
G

F

A

I

I

V

M

L

L

A

A

A

M

T

V

L

L

S

Q

A

S

V

V

G

G

-

L

-

D

-

L

-

T

-

P

S

G

L

S

P

P

V

V

A

A

-

L

G

A

L

Y

A

A

L

M

I

I

L

L

G

G

I

I

D

D

R

A

F

I

M

L

S
x
D

E

M

A

G

R
|
R

A
x
T

L

M

T

V

N

N

L

V

V

T

G

G

N

D

A

L

V

A

A

G

T

T

L

V

V

I

V

V

A

A

K

K

W

T

V

E

K

K

E

E

L

L

D

D

E

E

D

S

binding (3s)-3-(benzyloxy)-l-aspartic acid: R274 (≠ S269), S275 (= S270), S276 (= S271), T307 (≠ S301), M310 (≠ L304), T313 (= T307), G358 (= G352), D394 (≠ S382), R397 (= R385), T398 (≠ A386)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
30% identity, 91% coverage: 7:410/444 of query aligns to 4:411/416 of 6r7rA

query
sites
6r7rA

L

L

Y

W

K

K

S

I

L

L

Y

W

F

G

Q

L

V

V

I

L

V

G

A

A

I

V

A

-

I

F

G

G

I

L

L

I

L

A

G

G

H
|
H

F

F

Y

G

P

Y

Q

A

T

G

G

A

V

V

A

K

-

T

-

Y

L

I

K

K

P

P

L

F

G

G

D

D

G

L

F

F

I

V

K

R

L

L

I

L

K

K

M

M

V

L

I

V

A

M

P

P

I

I

I

V

F

L

C

A

T

S

V

L

V

V

S

V

G

G

I

A

A

A

G

S

M

I

Q

S

N

P

M

A

K

R

S

-

V

L

G

G

K

R

T

V

G

G

G

V

Y

K

A

I

L

V

Y

Y

F

Y

E

L

I

A

V

T

S

S

T

A

I

M

A

A

L

V

L

F

I

F