SitesBLAST

3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
39% identity, 93% coverage: 3:241/256 of query aligns to 55:290/293 of 3ws7A

query
sites
3ws7A

A

A

G

G

A

V

V

Y

A

V

L

A

A

E

N

S

P

P

K

A

E

D

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L

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A

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I

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M
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M

V
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V

P
x
S

D

D

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A

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P

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x
D

V

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E

D
x
Q

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L

F

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A

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A

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E

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L

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I

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V

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V

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D

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M

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F

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A

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Q

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Y

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G

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I

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E

Y

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L

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D

A

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P

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V

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x
T

G
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G

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E

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K

G

G

A

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I

A

E

A

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M

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K

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A

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P

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Y

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G

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K

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A

A

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L

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F

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x
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x
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K

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D

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L

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: M76 (= M24), V77 (= V25), S78 (≠ P26), D82 (≠ Q30), Q85 (≠ D33), V133 (= V81), F241 (= F192), K242 (≠ R193), H245 (≠ L196), K248 (= K199)
binding sulfate ion: T134 (≠ S82), G135 (= G83), K183 (= K131)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 20, 21, 22, 23, 24, 43, 44, 45, 48

P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
35% identity, 100% coverage: 1:256/256 of query aligns to 41:297/298 of P0A9V8

query
sites
P0A9V8

V

V

A

D

A

K

G

G

A

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V

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A

P

L

A

A

A

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N

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A

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K

E

D

A

A

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F

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M

V
x
L

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P

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D

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L

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R

D

N

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L

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F

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x
T

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P

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L

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x
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A

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x
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L65 (≠ V25) binding
T96 (≠ S56) binding
G122 (≠ S82) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
R123 (≠ G83) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
T124 (≠ G84) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
NNYMS 174:178 (≠ NQII- 134:137) binding
K240 (= K199) binding

Sites not aligning to the query:

11:12 binding
31 binding

6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
36% identity, 95% coverage: 1:243/256 of query aligns to 40:283/294 of 6smyA

query
sites
6smyA

V

V

A

D

A

K

G

G

A

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A

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A

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N

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K

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x
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E

binding (2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic acid: F232 (= F192), W278 (≠ H238)

6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
36% identity, 95% coverage: 1:243/256 of query aligns to 40:283/295 of 6smzC

query
sites
6smzC

V

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F

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N

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C

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S

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x
T

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G

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x
R

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N

Q

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S

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G

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A

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M

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A

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A

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K

K

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G

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M

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P

G

A

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M

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H

A

Q

H

K

K

D

D

L

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N

G

L

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A

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H

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M

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G

A

A

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F

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C

A

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A

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A

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G

N

R

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H

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A

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L

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L

K

E

binding nicotinamide-adenine-dinucleotide: M63 (= M24), L64 (≠ V25), V73 (= V34), S94 (= S55), T95 (≠ S56), R122 (≠ G83)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 10, 11, 29, 30, 31

P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
31% identity, 97% coverage: 3:251/256 of query aligns to 80:335/335 of P29266

query
sites
P29266

A

A

G

G

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E

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S

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A

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N

G

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I

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D

M

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S

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P

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A

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K

K

A

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F

L

A

A

A

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K

E

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N

E

E

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K

M

G

G

A

A

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F

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D

D

A

A

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P

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G

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A

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L

L

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M

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N

A

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F

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A

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E

L

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C

M

M

G

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N

N

I

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Y

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C

G

G

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A

N

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G

G

Q

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A

A

K
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K

V

I

A

C

N

N

Q
x
N

I

M

I

L

V

L

A

A

L

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S

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M

Q

I

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A

A

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E

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F

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S

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D

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P

A

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A

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K

A

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M

-

G

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N

F

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A

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W

E

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P

G

G

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M

D

D

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G

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V

P

P

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-

N

-

Y

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Q

-

G

G

G

F

F

R

G

I

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T

L

L

H

M

Q

A

K

K

D

D

L

L

N

G

L

L

A

A

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Q

A

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A

A

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L

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K

I

T

N

P

L

I

P

L

N

L

T

G

A

S

N

V

T

A

Q

H

Q

Q

V

I

F

Y

S

R

T

M

C

M

A

C

A

S

I

K

G

G

G

Y

S

S

N

K

W

K

D

D

H

F

S

S

A

S

L

V

I

F

K

Q

G

Y

L

L

E

R

H

E

M

E

A

E

N

T

F

F

K208 (= K131) mutation K->A,H,N,R: Complete loss of activity.
N212 (≠ Q135) mutation to Q: Decrease in activity.

Sites not aligning to the query:

68 D→R: Decrease of activity with NAD, increase of activity with NADP.

P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
31% identity, 97% coverage: 3:251/256 of query aligns to 81:336/336 of P31937

query
sites
P31937

A

A

G

G

A

E

V

Q

A

V

L

V

A

S

N

S

P

P

K

A

E

D

V

V

A

A

Q

E

E

K

A

A

E

D

F

R

I

I

V

T

M

M

V
x
L

P
|
P

D

T

T

S

P

I

Q
x
N

V

A

D

I

D

E

V

A

L

Y

F

S

R

G

A

A

D

N

G

G

V

I

A

L

A

K

G

K

V

V

S

K

K

K

G

G

K

S

V

L

I

I

D

D

M

S

S

S

S
x
T

I

I

S

D

P

P

T

A

A

V

T

S

K

K

A

E

F

L

A

A

A

K

K

E

I

V

N

E

E

K

K

M

G

G

A

A

Q

V

Y

F

L

M

D

D

A

A

P

P

V

V

S

S

G

G

E

V

V

G

G

A

A

A

K

R

A

S

A

G

T

N

L

L

S

T

I

F

M

M

V

V

G

G

D

V

A

E

D

D

A

E

F

F

E

A

R

A

A

A

L

Q

P

E

L

L

F

L

Q

G

A

C

M

M

G

G

K

S

N

N

I

V

T

V

L

Y

V

C

G

G

G

A

N

V

G

G

D

T

G

G

Q

Q

T

A

A

A

K

K

V

I

A

C

N

N

Q

N

I

M

I

L

V

L

A

A

L

I

N

S

I

M

Q

I

A

G

V

T

A

A

E

E

A

A

L

M

L

N

F

L

A

G

S

I

K

R

N

L

G

G

A

L

D

D

P

P

A

K

K

L

V

L

R

A

E

K

A

I

L

L

M

-

G

-

G

N

F

M

A

S

S

S

S

G

K

R

I

C

L

W

E

S

V

S

H

D

G

T

E

Y

R

N

M

P

I

V

K

P

G

G

T

V

F

M

D

D

-

G

-

V

P

P

-

S

-

A

-

N

-

Y

-

Q

-

G

G

G

F

F

R

G

I

T

S

T

L

L

H

M

Q

A

K
|
K

D

D

L

L

N

G

L

L

A

A

L

Q

A

D

G

S

A

A

R

T

E

S

L

T

G

K

I

S

N

P

L

I

P

L

N

L

T

G

A

S

N

L

T

A

Q

H

Q

Q

V

I

F

Y

S

R

T

M

C

M

A

C

A

A

I

K

G

G

G

Y

S

S

N

K

W

K

D

D

H

F

S

S

A

S

L

V

I

F

K

Q

G

F

L

L

E

R

H

E

M

E

A

E

N

T

F

F

LP 103:104 (≠ VP 25:26) binding
N108 (≠ Q30) binding
T134 (≠ S56) binding
K284 (= K199) binding

Sites not aligning to the query:

1:36 modified: transit peptide, Mitochondrion
40:68 binding

3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
31% identity, 96% coverage: 1:246/256 of query aligns to 41:286/287 of 3pduA

query
sites
3pduA

V

V

A

A

A

L

G

G

A

A

V

R

A

Q

L

A

A

S

N

S

P

P

K

A

E

E

V

V

A

C

Q

A

E

A

A

C

E

D

F

I

I

T

I

I

V

A

M
|
M

V
x
L

P
x
A

D

D

T

P

P

A

Q
x
A

V

A

D

R

D

E

V

V

L

C

F

F

R

G

A

A

D

N

G

G

V

V

A

L

A

E

G

G

V

I

S

G

K

G

G

G

K

R

V

G

V

Y

I

I

D

D

M

M

S

S

S
x
T

I

V

S

D

P

D

T

E

A

T

T

S

K

T

A

A

F

I

A

G

A

A

K

A

I

V

N

T

E

A

K

R

G

G

A

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V
|
V

S

S

G
|
G

G
x
T

E

K

V

K

G

P

A

A

K

E

A

D

A

G

T

T

L

L

S

I

I

I

M

L

V

A

G

A

G

G

D

D

A

Q

D

S

A

L

F

F

E

T

R

D

A

A

L

G

P

P

L

A

F

F

Q

A

A

A

M

L

G

G

K

K

N

K

I

C

T

L

L

H

V

L

G

G

G

E

N

V

G

G

D

Q

G

G

Q

A

T

R

A

M

K
|
K

V

L

A

V

N

V

Q

N

I

M

I

I

V

M

A

G

L

Q

N

M

I

M

Q

T

A

A

V

L

A

G

E

E

A

G

L

M

L

A

F

L

A

G

S

R

K

N

N

C

G

G

A

L

D

D

P

G

A

G

K

Q

V

L

R

L

E

E

A

V

L

L

M

D

G

A

G

G

F

A

A

M

S

A

S

N

K

P

I

M

L

F

E

K

V

G

H

K

G

G

E

Q

R

M

M

L

I

L

K

S

G

G

T

E

F

F

D

P

P

T

G
x
S

F
|
F

R
x
P

I

L

S

K

L
x
H

H

M

Q

Q

K
|
K

D

D

L

L

N
x
R

L

L

A

A

L

V

A
x
E

G

L

A

G

R

D

E
x
R

L

L

G

G

I

Q

N

P

L

L

P

H

N

G

T

A

A

A

N

T

T

A

Q

N

Q

E

V

S

F

F

S

K

T

R

C

A

A

R

A

A

I

A

G

G

G

H

S

A

N

D

W

E

D

D

H

F

S

A

A

A

L

V

I

F

K

R

G

V

L

L

E

E

binding glycerol: R242 (≠ N202), E246 (≠ A206), E246 (≠ A206), R250 (≠ E210)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M64 (= M24), L65 (≠ V25), A66 (≠ P26), A70 (≠ Q30), T96 (≠ S56), V121 (= V81), G123 (= G83), T124 (≠ G84), K171 (= K131), S231 (≠ G191), F232 (= F192), P233 (≠ R193), H236 (≠ L196), K239 (= K199)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 8, 10, 11, 12, 31, 32, 33

5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
32% identity, 94% coverage: 4:243/256 of query aligns to 47:287/294 of 5je8B

query
sites
5je8B

G

G

A

I

V

I

A

G

L

L

A

S

N

I

P

S

K

K

E

-

V

L

A

A

Q

E

E

T

A

C

E

D

F

V

I

V

I

F

V

T

M

S

V
x
L

P
|
P

D

S

T

P

P

R

Q

A

V

V

D

E

D

A

V
|
V

L

Y

F

F

R

G

A

A

D

E

G

G

V

L

A

F

A

E

G

N

V

G

S

H

K

S

G

N

K

V

V

V

V

F

I

I

D

D

M

T

S
|
S

S

T

I

V

S

S

P

P

T

Q

A

L

T

N

K

K

A

Q

F

L

A

E

K

A

I

A

N

K

E

E

K

K

G

K

A

V

Q

D

Y

F

L

L

D

A

A

A

P

P

V
|
V

S

S

G

G

E

V

V

I

G

G

A

A

K

E

A

N

A

R

T

T

L

L

S

T

I

F

M

M

V

V

G

G

D

S

A

K

D

D

A

V

F

Y

E

E

R

K

A

T

L

E

P

S

L

I

F

M

Q

G

A

V

M

L

G

G

K

A

N

N

I

I

T

F

L

H

V

V

G

S

G

E

N

Q

G

I

D

D

-

S

G

G

Q

T

T

T

A

V

K

K

V

L

A

I

N

N

Q

N

I

L

V

I

A

G

L

F

N

Y

I

T

Q

A

A

G

V

V

A

S

E

E

A

A

L

L

L

T

F

L

A

A

S

K

K

K

N

N

G

N

A

M

D

D

P

L

A

D

K

K

V

M

R

F

E

D

A

I

L

L

M

N

G

V

G

S

F

Y

A

G

S

Q

S

S

K

R

I

I

L

Y

E

E

V

R

H

N

G

Y

E

K

R

S

M

F

I

I

-

A

K

P

G

E

T

N

F

Y

D

E

P

P

G

G

F

F

R

T

I

V

S

N

L

L

H

L

Q

K

K

K

D

D

L

L

N

G

L

F

A

A

L

V

A

D

G

L

A

A

R

K

E

E

L

S

G

E

I

L

N

H

L

L

P

P

N

V

T

S

A

E

N

M

T

L

Q

L

Q

N

V

V

F

Y

S

D

T

E

C

A

A

S

A

Q

I

A

G

G

G

Y

S

G

N

E

W

N

D

D

H

M

S

A

A

A

L

L

I

Y

K

K

binding nicotinamide-adenine-dinucleotide: L67 (≠ V25), P68 (= P26), V76 (= V34), S97 (= S55), V123 (= V81)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 12, 13, 14, 33, 34

2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
31% identity, 96% coverage: 3:247/256 of query aligns to 42:293/296 of 2i9pB

query
sites
2i9pB

A

A

G

G

A

E

V

Q

A

V

L

V

A

S

N

S

P

P

K

A

E

D

V

V

A

A

Q

E

E

K

A

A

E

D

F

R

I

I

V

T

M
|
M

V
x
L

P
|
P

D

T

T

S

P

I

Q

N

V

A

D

I

D

E

V

A

L

Y

F

S

R

G

A

A

D

N

G

G

V

I

A

L

A

K

G

K

V

V

S

K

K

K

G

G

K

S

V

L

I

I

D

D

M

S

S

S

S
x
T

I

I

S

D

P

P

T

A

A

V

T

S

K

K

A

E

F

L

A

A

A

K

K

E

I

V

N

E

E

K

K

M

G

G

A

A

Q

V

Y

F

L

M

D

D

A

A

P

P

V
|
V

S

S

G
|
G

G

G

E

V

V

G

G

A

A

A

K

R

A

S

A

G

T

N

L

L

S

T

I

F

M

M

V

V

G

G

D

V

A

E

D

D

A

E

F

F

E

A

R

A

A

A

L

Q

P

E

L

L

F

L

Q

G

A

C

M

M

G

G

K

S

N

N

I

V

T

V

L

Y

V

C

G

G

G

A

N

V

G

G

D

T

G

G

Q

Q

T

A

A

A

K

K

V

I

A

C

N

N

Q

N

I

M

I

L

V

L

A

A

L

I

N

S

I

M

Q

I

A

G

V

T

A

A

E

E

A

A

L

M

L

N

F

L

A

G

S

I

K

R

N

L

G

G

A

L

D

D

P

P

A

K

K

L

V

L

R

A

E

K

A

I

L

L

M

-

G

-

G

N

F

M

A

S

S

S

S

G

K

R

I

C

L

W

E

S

V

S

H

D

G

T

E

Y

R

N

M

P

I

V

K

P

G

G

T

V

F

M

D

D

-

G

-

V

P

P

-

S

-

A

-

N

-

Y

-

Q

-

G

G

G

F
|
F

R

G

I

T

S

T

L

L

H

M

Q

A

K
|
K

D

D

L

L

N

G

L

L

A

A

L

Q

A

D

G

S

A

A

R

T

E

S

L

T

G

K

I

S

N

P

L

I

P

L

N

L

T

G

A

S

N

L

T

A

Q

H

Q

Q

V

I

F

Y

S

R

T

M

C

M

A

C

A

A

I

K

G

G

G

Y

S

S

N

K

W

K

D

D

H

F

S

S

A

S

L

V

I

F

K

Q

G

F

L

L

E

R

H

E

binding nicotinamide-adenine-dinucleotide: M63 (= M24), L64 (≠ V25), P65 (= P26), T95 (≠ S56), V120 (= V81), G122 (= G83), F238 (= F192), K245 (= K199)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 10, 11, 29, 30, 31

3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
29% identity, 96% coverage: 2:247/256 of query aligns to 42:287/287 of 3pefA

query
sites
3pefA

A

A

A

L

G

G

A

A

V

E

A

R

L

A

A

A

N

T

P

P

K

C

E

E

V

V

A

V

Q

E

E

S

A

C

E

P

F

V

I

T

I

F

V

A

M
|
M

V
x
L

P
x
A

D
|
D

T

P

P

A

Q
x
A

V

A

D

E

D
x
E

V

V

L

C

F

F

R

G

A

K

D

H

G

G

V

V

A

L

A

E

G

G

V

I

S

G

K

E

G

G

K

R

V

G

V

Y

I

V

D

D

M

M

S

S

S
x
T

I

V

S

D

P

P

T

A

A

T

T

S

K

Q

A

R

F

I

A

G

A

V

K

A

I

V

N

V

E

A

K

K

G

G

A

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V
|
V

S

S

G
|
G

G
x
S

E

K

V

K

G

P

A

A

K

E

A

D

A

G

T

T

L

L

S

I

I

I

M

L

V

A

G

A

G

G

D

D

A

R

D

N

A

L

F

Y

E

D

R

E

A

A

L

M

P

P

L

G

F

F

Q

E

A

K

M

M

G

G

K

K

N

K

I

I

T

I

L

H

V

L

G

G

G

D

N

V

G

G

D

K

G

G

Q

A

T

E

A

M

K
|
K

V

L

A

V

N

V

Q
x
N

I

M

I

V

V
x
M

A

G

L

G

N

M

I

M

Q

A

A

C

V

F

A

C

E

E

A

G

L

L

L

A

F

L

A

G

S

E

K

K

N

A

G

G

A

L

D

A

P

T

A

D

K

A

V

I

R
x
L

E

D

A

V

L

I

M
x
G

G

A

G

G

F

A

A

M

S

A

S
x
N

K

P

I

M

L

F

E
x
A

V

L

H

K

G

G

E

G

R

L

M

I

I

R

K

D

G

R

T

N

F

F

D

A

P

P

G
x
A

F
|
F

R

P

I

L

S

K

L
x
H

H

M

Q

Q

K
|
K

D

D

L

L

N
x
R

L

L

A

A

L

V

A

A

G

L

A

G

R

D

E

R

L

V

G

G

I

Q

N

P

L

L

P

V

N

A

T

S

A

A

N

A

T

A

Q

N

Q

E

V

L

F

F

S

K

T

G

C

A

A
x
R

A

A

I

A

G

G

G

F

S

G

N

D

W

E

D

D

H

F

S

S

A

A

L

I

I

F

K

K

G

T

L

Y

E

E

H

R

binding glycerol: D67 (= D27), G123 (= G83), K171 (= K131), N175 (≠ Q135), M178 (≠ V138), L203 (≠ R163), G207 (≠ M167), N213 (≠ S173), A217 (≠ E177), F232 (= F192), H236 (≠ L196), K239 (= K199), R242 (≠ N202), R269 (≠ A229)
binding nadp nicotinamide-adenine-dinucleotide phosphate: M64 (= M24), L65 (≠ V25), A66 (≠ P26), A70 (≠ Q30), E73 (≠ D33), T96 (≠ S56), V121 (= V81), G123 (= G83), S124 (≠ G84), A231 (≠ G191), F232 (= F192), H236 (≠ L196), K239 (= K199)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 31, 32, 33, 36

1wp4A Structure of tt368 protein from thermus thermophilus hb8 (see paper)
32% identity, 96% coverage: 9:254/256 of query aligns to 46:288/288 of 1wp4A

query
sites
1wp4A

A

A

N

V

P

P

K

L

E

E

V

R

A

V

Q

A

E

E

A

A

E

R

F

V

I

I

I

F

V

T

M
x
C

V
x
L

P
|
P

D

T

T

T

P

R

Q
x
E

V

V

D

Y

D

E

V

V

L

-

F

-

R

-

A

A

D

E

G

A

V

L

A

Y

A

P

G

Y

V

L

S

R

K

E

G

G

K

T

V

Y

V

W

I

V

D

D

M

A

S

T

S
|
S

I

G

S

E

P

P

T

E

A

A

T

S

K

R

A

R

F

L

A

A

A

E

K

R

I

L

N

R

E

E

K

K

G

G

A

V

Q

T

Y

Y

L

L

D

D

A

A

P

P

V
|
V

S
|
S

G
|
G

E

T

V

S

G

G

A

A

K

E

A

A

A

G

T

T

L

L

S

T

I

V

M

M

V

L

G

G

D

P

A

E

D

E

A

A

F

V

E

E

R

R

A

V

L

R

P

P

L

-

F

F

Q

L

A

A

M

Y

G

A

K

K

N

K

I

V

T

V

L

H

V

V

G

G

G

P

N

V

G

G

D

A

G

G

Q

H

T

A

A

V

K
|
K

V

A

A

I

N
|
N

Q
x
N

I

A

I

L

V

L

A

A

L

V

N

N

I

L

Q

W

A

A

V

A

A

G

E

E

A

G

L

L

F

A

A

L

S

V

K

K

N

Q

G

G

A

V

D

S

P

A

A

E

K

K

V

A

R

L

E

E

A

V

L

I

M

N

G

A

G

S

F

S

A

G

S

R

S

S

K

N

I

A

L

T

E

E

-

N

V

L

H

I

G

P

E

Q

R

R

M

V

I

L

K

T

G

R

T

A

F

F

D

P

P

K

G
x
T

F
|
F

R

A

I

L

S

G

L

L

H

L

Q

V

K
|
K

D

D

L

L

N

G

L

I

A

A

L

M

A

G

G

V

A

L

R

D

E

G

L

E

G

K

I

A

N

P

L

S

P

P

N

L

T

L

A

R

N

L

T

A

Q

R

Q

E

V

V

F

Y

S

E

T

M

C

A

A

K

A

R

I

E

G

L

G

G

S

P

N

D

W

A

D

D

H

H

S

V

A

E

L

A

I

L

K

R

G

L

L

L

E

E

H

R

M

W

A

G

N

G

F

V

S

E

I

I

R

R

active site: S116 (= S82), K164 (= K131), N167 (= N134), N168 (≠ Q135)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C61 (≠ M24), L62 (≠ V25), P63 (= P26), E67 (≠ Q30), S90 (= S56), V115 (= V81), T225 (≠ G191), F226 (= F192), K233 (= K199)
binding sulfate ion: S116 (= S82), G117 (= G83), G118 (= G84), K164 (= K131)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 7, 8, 9, 10, 11, 29, 30, 31, 34

2cvzC Structure of hydroxyisobutyrate dehydrogenase from thermus thermophilus hb8 (see paper)
32% identity, 96% coverage: 9:254/256 of query aligns to 47:289/289 of 2cvzC

query
sites
2cvzC

A

A

N

V

P

P

K

L

E

E

V

R

A

V

Q

A

E

E

A

A

E

R

F

V

I

I

I

F

V

T

M
x
C

V
x
L

P
|
P

D

T

T

T

P

R

Q
x
E

V

V

D

Y

D
x
E

V

V

L

-

F

-

R

-

A

A

D

E

G

A

V

L

A

Y

A

P

G

Y

V

L

S

R

K

E

G

G

K

T

V

Y

V

W

I

V

D

D

M

A

S

T

S
|
S

I

G

S

E

P

P

T

E

A

A

T

S

K

R

A

R

F

L

A

A

A

E

K

R

I

L

N

R

E

E

K

K

G

G

A

V

Q

T

Y

Y

L

L

D

D

A

A

P

P

V
|
V

S
|
S

G

G

E

T

V

S

G

G

A

A

K

E

A

A

A

G

T

T

L

L

S

T

I

V

M

M

V

L

G

G

D

P

A

E

D

E

A

A

F

V

E

E

R

R

A

V

L

R

P

P

L

-

F

F

Q

L

A

A

M

Y

G

A

K

K

N

K

I

V

T

V

L

H

V

V

G

G

G

P

N

V

G

G

D

A

G

G

Q

H

T

A

A

V

K
|
K

V

A

A

I

N
|
N

Q
x
N

I

A

I

L

V

L

A

A

L

V

N

N

I

L

Q

W

A

A

V

A

A

G

E

E

A

G

L

L

F

A

A

L

S

V

K

K

N

Q

G

G

A

V

D

S

P

A

A

E

K

K

V

A

R

L

E

E

A

V

L

I

M

N

G

A

G

S

F

S

A

G

S

R

S

S

K

N

I

A

L

T

E

E

-

N

V

L

H

I

G

P

E

Q

R

R

M

V

I

L

K

T

G

R

T

A

F

F

D

P

P

K

G

T

F
|
F

R

A

I

L

S

G

L

L

H

L

Q

V

K
|
K

D

D

L

L

N

G

L

I

A

A

L

M

A

G

G

V

A

L

R

D

E

G

L

E

G

K

I

A

N

P

L

S

P

P

N

L

T

L

A

R

N

L

T

A

Q

R

Q

E

V

V

F

Y

S

E

T

M

C

A

A

K

A

R

I

E

G

L

G

G

S

P

N

D

W

A

D

D

H

H

S

V

A

E

L

A

I

L

K

R

G

L

L

L

E

E

H

R

M

W

A

G

N

G

F

V

S

E

I

I

R

R

active site: S117 (= S82), K165 (= K131), N168 (= N134), N169 (≠ Q135)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C62 (≠ M24), L63 (≠ V25), P64 (= P26), E68 (≠ Q30), E71 (≠ D33), S91 (= S56), V116 (= V81), F227 (= F192), K234 (= K199)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 8, 9, 10, 11, 12, 30, 31, 32

Q9I5I6 NAD-dependent L-serine dehydrogenase; L-serine 3-dehydrogenase (NAD(+)); EC 1.1.1.387 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
37% identity, 73% coverage: 16:203/256 of query aligns to 56:250/298 of Q9I5I6

query
sites
Q9I5I6

Q

Q

E

G

A

A

E

D

F

V

I

V

I

I

V

S

M

M

V

L

P
|
P

D

A

T

S

P

Q

Q

H

V

V

D

E

D

G

V

L

L

Y

F

L

R

D

A

D

D

D

G

G

V

L

A

L

A

A

G

H

V

I

S

A

K

P

G

G

K

T

V

L

V

V

I

L

D

E

M

C

S

S

S
x
T

I

I

S

A

P

P

T

T

A

S

T

A

K

R

A

K

F

I

A

H

A

A

K

A

I

A

N

R

E

E

K

R

G

G

A

L

Q

A

Y

M

L

L

D

D

A

A

P

P

V

V

S
|
S

G

G

E

T

V

A

G

G

A

A

K

A

A

A

A

G

T

T

L

L

S

T

I

F

M

M

V

V

G

G

D

D

A

A

D

E

A

A

F

L

E

E

R

K

A

A

L

R

P

P

L

L

F

F

Q

E

A

A

M

M

G

G

K

R

N

N

I

I

T

F

L

H

V

A

G

G

G

P

N

D

G

G

D

A

G

G

Q

Q

T

V

A

A

K
|
K

V

V

A

C

N

N

Q
x
N

I

Q

I

L

V

L

A

A

L

V

N

L

I

M

Q

I

A

G

V

T

A

A

E

E

A

A

L

M

L

A

F

L

A

G

S

V

K

A

N

N

G

G

A

L

D

E

P

A

A

K

K

V

V

L

R

A

E

E

A

I

L

M

M

R

G

R

G

S

F

S

A

G

S

G

S

N

K
x
W

I

A

L

L

E

E

V

V

H
x
Y

G

N

E

P

R

W

-

P

-

G

-

V

-

M

-

E

-

N

-

A

M

P

I

A

K

S

G

R

T

D

F

Y

D

S

P

G

G

G

F

F

R

M

I

A

S

Q

L

L

H

M

Q

A

K
|
K

D
|
D

L

L

N

G

L

L

P66 (= P26) binding
T96 (≠ S56) binding ; mutation to A: Almost abolished activity.
S122 (= S82) mutation to A: Strongly reduced activity.
K171 (= K131) active site
N175 (≠ Q135) mutation to A: Strongly reduced activity.
W214 (≠ K174) mutation to A: Almost abolished activity.
Y219 (≠ H179) mutation to A: Strongly reduced activity.
K246 (= K199) binding ; mutation to A: Almost abolished activity.
D247 (= D200) mutation to A: Almost abolished activity.

Sites not aligning to the query:

2:31 binding

3q3cA Crystal structure of a serine dehydrogenase from pseudomonas aeruginosa pao1 in complex with NAD (see paper)
38% identity, 73% coverage: 16:203/256 of query aligns to 55:248/294 of 3q3cA

query
sites
3q3cA

Q

Q

E

G

A

A

E

D

F

V

I

V

I

I

V

S

M
|
M

V
x
L

P
|
P

D

A

T

S

P

Q

Q

H

V

V

D

E

D

G

V

-

L

L

F

Y

R

L

A

D

D

D

G

G

V

L

A

L

A

A

G

H

V

I

S

A

K

P

G

G

K

T

V

L

V

V

I

L

D

E

M

C

S

S

S
x
T

I

I

S

A

P

P

T

T

A

S

T

A

K

R

A

K

F

I

A

H

A

A

K

A

I

A

N

R

E

E

K

R

G

G

A

L

Q

A

Y

M

L

L

D

D

A

A

P

P

V
|
V

S

S

G
|
G

G

G

E

T

V

A

G

G

A

A

K

A

A

A

A

G

T

T

L

L

S

T

I

F

M

M

V

V

G

G

D

D

A

A

D

E

A

A

F

L

E

E

R

K

A

A

L

R

P

P

L

L

F

F

Q

E

A

A

M

M

G

G

K

R

N

N

I

I

T

F

L

H

V

A

G

G

G

P

N

D

G

G

D

A

G

G

Q

Q

T

V

A

A

K

K

V

V

A

C

N

N

Q

N

I

Q

I

L

V

L

A

A

L

V

N

L

I

M

Q

I

A

G

V

T

A

A

E

E

A

A

L

M

L

A

F

L

A

G

S

V

K

A

N

N

G

G

A

L

D

E

P

A

A

K

K

V

V

L

R

A

E

E

A

I

L

M

M

R

G

R

G

S

F

S

A

G

S

G

S

N

K

W

I

A

L

L

E

E

V

V

H

Y

G

N

E

P

R

W

-

P

-

G

-

V

-

M

-

E

-

N

-

A

M

P

I

A

K

S

G

R

T

D

F

Y

D

S

P

G

G

G

F
|
F

R

M

I

A

S

Q

L

L

H

M

Q

A

K
|
K

D

D

L

L

N

G

L

L

binding nicotinamide-adenine-dinucleotide: M63 (= M24), L64 (≠ V25), P65 (= P26), T94 (≠ S56), V119 (= V81), G121 (= G83), F237 (= F192), K244 (= K199)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 10, 11, 29, 30, 31

3obbA Crystal structure of a possible 3-hydroxyisobutyrate dehydrogenase from pseudomonas aeruginosa pao1 (see paper)
37% identity, 73% coverage: 16:203/256 of query aligns to 56:249/295 of 3obbA

query
sites
3obbA

Q

Q

E

G

A

A

E

D

F

V

I

V

I

I

V

S

M

M

V

L

P

P

D

A

T

S

P

Q

Q

H

V

V

D

E

D

G

V

L

L

Y

F

L

R

D

A

D

D

D

G

G

V

L

A

L

A

A

G

H

V

I

S

A

K

P

G

-

K

T

V

L

V

V

I

L

D

E

M

C

S

S

S

T

I

I

S

A

P

P

T

T

A

S

T

A

K

R

A

K

F

I

A

H

A

A

K

A

I

A

N

R

E

E

K

R

G

G

A

L

Q

A

Y

M

L

L

D

D

A

A

P

P

V

V

S

S

G
|
G

E

T

V

A

G

G

A

A

K

A

A

A

A

G

T

T

L

L

S

T

I

F

M

M

V

V

G

G

D

D

A

A

D

E

A

A

F

L

E

E

R

K

A

A

L

R

P

P

L

L

F

F

Q

E

A

A

M

M

G

G

K

R

N

N

I

I

T

F

L

H

V

A

G

G

G

P

N

D

G

G

D

A

G

G

Q

Q

T

V

A

A

K
|
K

V

V

A

C

N

N

Q
x
N

I

Q

I

L

V

L

A

A

L

V

N

L

I

M

Q

I

A

G

V

T

A

A

E

E

A

A

L

M

L

A

F

L

A

G

S

V

K

A

N

N

G

G

A

L

D

E

P

A

A

K

K

V

V

L

R

A

E

E

A

I

L

M

M

R

G

R

G

S

F

S

A

G

S

G

S

N

K
x
W

I

A

L

L

E

E

V

V

H
x
Y

G

N

E

P

R

W

-

P

-

G

-

V

-

M

-

E

-

N

-

A

M

P

I

A

K

S

G

R

T

D

F

Y

D

S

P

G

G

G

F
|
F

R

M

I

A

S

Q

L

L

H

M

Q

A

K

K

D

D

L

L

N

G

L

L

binding 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid: G122 (= G83), G123 (= G84), K170 (= K131), N174 (≠ Q135), W213 (≠ K174), Y218 (≠ H179), F238 (= F192)

2uyyA Structure of the cytokine-like nuclear factor n-pac
27% identity, 93% coverage: 10:247/256 of query aligns to 55:292/292 of 2uyyA

query
sites
2uyyA

N

T

P

P

K

A

E

E

V

V

A

V

Q

S

E

T

A

C

E

D

F

I

I

T

I

F

V

A

M

C

V
|
V

P
x
S

D

D

T

P

P

K

Q
x
A

V

A

D

K

D

D

V

L

L

V

F

L

R

G

A

P

D

S

G

G

V

V

A

L

A

Q

G

G

V

I

S

R

K

P

G

G

K

K

V

C

V

Y

I

V

D

D

M

M

S

S

S
x
T

I

V

S

D

P

A

T

D

A

T

T

V

K

T

A

E

F

L

A

A

A

Q

K

V

I

I

N

V

E

S

K

R

G

G

A

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

V

Q

G

L

A

S

K

N

A

D

A

G

T

M

L

L

S

V

I

I

M

L

V

A

G

A

G

G

D

D

A

R

D

G

A

L

F

Y

E

E

R

D

A

C

L

S

P

S

L

C

F

F

Q

Q

A

A

M

M

G

G

K

K

N

T

I

S

T

F

L

F

V

L

G

G

G

E

N

V

G

G

D

N

G

A

Q

A

T

K

A

M

K

M

V

L

A

I

N

V

Q

N

I

M

I

V

V

Q

A

G

L

S

N

F

I

M

Q

A

A

T

V

I

A

A

E

E

A

G

L

L

L

T

F

L

A

A

S

Q

K

V

N

T

G

G

A

Q

D

S

P

Q

A

Q

K

T

V

L

R

L

E

D

A

I

L

L

M

N

G

Q

G

G

F

Q

A

L

S

A

S

S

K

I

I

F

L

L

E

D

V

Q

H

K

G

C

E

Q

R

N

M

I

I

L

K

Q

G

G

T

N

F

F

D

K

P

P

G

D

F
|
F

R
x
Y

I

L

S

K

L
x
Y

H

I

Q

Q

K
|
K

D

D

L

L

N

R

L

L

A

A

L

I

A

A

G

L

A

G

R

D

E

A

L

V

G

N

I

H

N

P

L

T

P

P

N

M

T

A

A

A

N

A

T

A

Q

N

Q

E

V

V

F

Y

S

K

T

R

C

A

A

K

A

A

I

L

G

D

G

Q

S

S

N

D

W

N

D

D

H

M

S

S

A

A

L

V

I

Y

K

R

G

A

L

Y

E

I

H

H

binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V25), S71 (≠ P26), A75 (≠ Q30), T101 (≠ S56), F237 (= F192), Y238 (≠ R193), Y241 (≠ L196), K244 (= K199)

Sites not aligning to the query:

binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: 15, 16, 17, 36, 37, 38

Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
27% identity, 96% coverage: 1:247/256 of query aligns to 307:553/553 of Q49A26

query
sites
Q49A26

V

I

A

Q

A

E

G

G

A

A

V

R

A

L

L

G

A

R

N

T

P

P

K

A

E

E

V

V

A

V

Q

S

E

T

A

C

E

D

F

I

I

T

I

F

V

A

M

C

V

V

P

S

D

D

T

P

P

K

Q

A

V

A

D

K

D

D

V

L

L

V

F

L

R

G

A

P

D

S

G

G

V

V

A

L

A

Q

G

G

V

I

S

R

K

P

G

G

K

K

V

C

V

Y

I

V

D

D

M

M

S

S

S
x
T

I

V

S

D

P

A

T

D

A

T

T

V

K

T

A

E

F

L

A

A

A

Q

K

V

I

I

N

V

E

S

K

R

G

G

A

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

V

Q

G

L

A

S

K

N

A

D

A

G

T

M

L

L

S

V

I

I

M

L

V

A

G

A

G

G

D

D

A

R

D

G

A

L

F

Y

E

E

R

D

A

C

L

S

P

S

L

C

F

F

Q

Q

A

A

M

M

G

G

K

K

N

T

I

S

T

F

L

F

V

L

G

G

G

E

N

V

G

G

D

N

G

A

Q

A

T

K

A

M

K
x
M

V

L

A

I

N

V

Q

N

I

M

I

V

V

Q

A

G

L

S

N

F

I

M

Q

A

A

T

V

I

A

A

E

E

A

G

L

L

L

T

F

L

A

A

S

Q

K

V

N

T

G

G

A

Q

D

S

P

Q

A

Q

K

T

V

L

R

L

E

D

A

I

L

L

M

N

G

Q

G

G

F

Q

A

L

S

A

S

S

K

I

I

F

L

L

E

D

V

Q

H

K

G

C

E

Q

R

N

M

I

I

L

K

Q

G

G

T

N

F

F

D

K

P
|
P

G

D

F

F

R

Y

I

L

S

K

L

Y

H

I

Q

Q

K
|
K

D

D

L

L

N

R

L

L

A

A

L

I

A

A

G

L

A

G

R

D

E

A

L

V

G

N

I

H

N

P

L

T

P

P

N

M

T

A

A

A

N

A

T

A

Q

N

Q

E

V

V

F

Y

S

K

T

R

C

A

A

K

A

A

I

L

G

D

G

Q

S

S

N

D

W

N

D

D

H

M

S

S

A

A

L

V

I

Y

K

R

G

A

L

Y

E

I

H

H

T362 (≠ S56) binding
M437 (≠ K131) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.
P496 (= P190) to L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
K505 (= K199) binding

Sites not aligning to the query:

214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
214:217 Interaction with histone H3
216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
216:225 Interaction with KDM1B
217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.
271:285 binding

Q922P9 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Putative oxidoreductase GLYR1 from Mus musculus (Mouse) (see paper)
27% identity, 95% coverage: 4:247/256 of query aligns to 303:546/546 of Q922P9

query
sites
Q922P9

G

G

A

A

V

R

A

L

L

G

A

R

N

T

P

P

K

A

E

E

V

V

A

V

Q

S

E

T

A

C

E

D

F

I

I

T

I

F

V

A

M

C

V

V

P

S

D

D

T

P

P

K

Q

A

V

A

D

K

D

D

V

L

L

V

F

L

R

G

A

P

D

S

G

G

V

V

A

L

A

Q

G

G

V

I

S

R

K

P

G

G

K

K

V

C

V

Y

I

V

D

D

M

M

S

S

S

T

I

V

S

D

P

A

T

D

A

T

T

V

K

T

A

E

F

L

A

A

A

Q

K

V

I

I

N

V

E

S

K

R

G

G

A

G

Q

R

Y

F

L

L

D

E

A

A

P

P

V

V

S

S

G

G

G

N

E

Q

V

Q

G

L

A

S

K

N

A

D

A

G

T

M

L

L

S

V

I

I

M

L

V

A

G

A

G

G

D

D

A

R

D

G

A

L

F

Y

E

E

R

D

A

C

L

S

P

S

L

C

F

F

Q

Q

A

A

M

M

G

G

K

K

N

T

I

S

T

F

L

F

V

L

G

G

G

E

N

V

G

G

D

N

G

A

Q

A

T

K

A

M

K

M

V

L

A

I

N

V

Q

N

I

M

I

V

V

Q

A

G

L

S

N

F

I

M

Q

A

A

T

V

I

A

A

E

E

A

G

L

L

L

T

F

L

A

A

S

Q

K

V

N

T

G

G

A

Q

D

S

P

Q

A

Q

K

T

V

L

R

L

E

D

A

I

L

L

M

N

G

Q

G

G

F

Q

A

L

S

A

S

S

K

I

I

F

L

L

E

D

V

Q

H

K

G

C

E

Q

R

N

M

I

I

L

K

Q

G

G

T

N

F

F

D

K

P
|
P

G

D

F

F

R

Y

I

L

S

K

L

Y

H

I

Q

Q

K

K

D

D

L

L

N

R

L

L

A

A

L

I

A

A

G

L

A

G

R

D

E

A

L

V

G

N

I

H

N

P

L

T

P

P

N

M

T

A

A

A

N

A

T

A

Q

N

Q

E

V

V

F

Y

S

K

T

R

C

A

A

K

A

A

I

L

G

D

G

Q

S

S

N

D

W

N

D

D

H

M

S

S

A

A

L

V

I

Y

K

R

G

A

L

Y

E

I

H

H

P489 (= P190) mutation to L: Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.

5y8iA Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + (s)-3-hydroxyisobutyrate (s-hiba) (see paper)
34% identity, 96% coverage: 5:250/256 of query aligns to 45:292/292 of 5y8iA

query
sites
5y8iA

A

A

V

V

A

F

L

R

A

S

N

A

P

P

K

E

E

A

V

V

A

A

Q

-

E

E

A

A

E

D

F

V

I

V

I

I

V

T

M

M

V

L

P

P

D

-

T

-

P

-

Q

-

V

T

D

G

D

E

V

V

L

V

F

R

R

R

A

C

-

Y

D

T

G

D

V

V

A

L

A

A

G

A

V

A

S

R

K

P

G

A

K

T

V

L

V

F

I

I

D

D

M

S

S

S

S

T

I

I

S

S

P

V

T

T

A

D

T

A

K

R

A

E

F

V

A

H

A

A

K

L

I

A

N

E

E

S

K

H

G

G

A

M

Q

L

Y

Q

L

L

D

D

A

A

P

P

V

V

S

S

G

G

E

V

V

K

G

G

A

A

K

A

A

A

T

T

L

L

S

A

I

F

M

M

V

V

G

G

D

D

A

E

D

S

A

T

F

L

E

R

R

R

A

A

L

R

P

P

L

V

F

L

Q
x
E

A

P

M

M

G
x
A

K

G

N
x
K

I
|
I

T

I

L

H

V

C

G

G

G

A

N

A

G

G

D

A

G

G

Q

Q

T

A

A

A

K

K

V

V

A

C

N

N

Q

N

I

M

I

V

V

L

A

A

L

V

N

Q

I

Q

Q

I

A

A

V

I

A

A

E

E

A

A

L

F

L

V

F

L

A

A

S

E

K

K

N

L

G

G

A

L

D

S

P

A

A

Q

K

S

V

L

R

F

E

D

A

V

L

I

M

T

G

G

G

-

F

-

A

A

S

T

S

G

K

N

I

C

L

W

E

A

V

V

H

H

G

T

E

N

R

C

M

P

I

V

K

P

G

G

T

P

-

V

-

P

-

T

-

S

-

P

-

A

-

N

-

D

F

F

D

K

P

P

G

G

F

F

R

S

I

T

S

A

L

L

H

M

Q

N

K

K

D

D

L

L

N

G

L

L

A

A

L

M

A

D

G

A

A

V

R

A

E

A

L

T

G

G

I

A

N

T

L

A

P

P

N

L

T

G

A

S

N

H

T

A

Q

A

Q

D

V

I

F

Y

S

A

T

K

C

F

A

A

I

-

G

D

G

H

S

A

N

D

W

L

D

D

H

F

S

S

A

A

L

V

I

I

K

H

G

T

L

L

E

R

H

A

M

R

A

A

N

D

binding (2~{S})-2-methylpentanedioic acid: E148 (≠ Q112), A151 (≠ G115), K153 (≠ N117), I154 (= I118)

Query Sequence

>Pf1N1B4_3659 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3659
VAAGAVALANPKEVAQEAEFIIVMVPDTPQVDDVLFRADGVAAGVSKGKVVIDMSSISPT
ATKAFAAKINEKGAQYLDAPVSGGEVGAKAATLSIMVGGDADAFERALPLFQAMGKNITL
VGGNGDGQTAKVANQIIVALNIQAVAEALLFASKNGADPAKVREALMGGFASSKILEVHG
ERMIKGTFDPGFRISLHQKDLNLALAGARELGINLPNTANTQQVFSTCAAIGGSNWDHSA
LIKGLEHMANFSIRDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory