SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_3813 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3813 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q9I2Y2 Phosphoserine phosphatase ThrH; PSP; PSPase; EC 3.1.3.3 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
89% identity, 100% coverage: 1:205/205 of query aligns to 1:205/205 of Q9I2Y2

query
sites
Q9I2Y2

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D7 (= D7) active site, Nucleophile; binding
E9 (= E9) active site, Proton donor; binding
D152 (= D152) binding

1rkvA Structure of phosphate complex of thrh from pseudomonas aeruginosa (see paper)
89% identity, 100% coverage: 1:205/205 of query aligns to 2:206/206 of 1rkvA

query
sites
1rkvA

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binding magnesium ion: D8 (= D7), E10 (= E9), D153 (= D152)
binding phosphate ion: D8 (= D7), E10 (= E9), S91 (= S90), D92 (= D91), K134 (= K133)

1rkuA Crystal structure of thrh gene product of pseudomonas aeruginosa (see paper)
89% identity, 100% coverage: 1:205/205 of query aligns to 2:206/206 of 1rkuA

query
sites
1rkuA

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binding magnesium ion: D8 (= D7), E10 (= E9), D153 (= D152)

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
32% identity, 87% coverage: 2:180/205 of query aligns to 10:180/200 of 4ap9A

query
sites
4ap9A

E

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active site: D15 (= D7), I16 (≠ L8), E17 (= E9), G103 (≠ D91), K141 (= K133), D162 (= D156)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ E22), I32 (≠ K23), T33 (= T24), L46 (≠ T32), W52 (≠ D38), D140 (= D131), K141 (= K133), Y160 (= Y154), A161 (≠ N155)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
24% identity, 68% coverage: 33:172/205 of query aligns to 32:176/200 of 1l7oA

query
sites
1l7oA

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Sites not aligning to the query:

binding zinc ion: 186, 188

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
30% identity, 60% coverage: 48:171/205 of query aligns to 230:360/409 of O53289

query
sites
O53289

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S273 (= S90) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K133) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D152) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D156) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
185 D→G: Completely abolishes enzymatic activity.; D→N: Completely abolishes enzymatic activity.
186 V→Q: Decreases enzymatic activity by 50%.
187 D→N: Decreases enzymatic activity by 15%.
188 S→A: No effect on enzymatic activity.

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
28% identity, 75% coverage: 39:192/205 of query aligns to 124:273/295 of 7qplA

query
sites
7qplA

Y

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P

N

T

E

L

E

L

R

C

A

H

N

R

R

L

L

I

I

T

-

D

D

A

G

G

T

R

R

V

L

T

T

G

G

Y

T

Q

V

L

A

R

E

Q

P

K

I

D

L

P

G

K

R

R

E

Q

A

S

K

V

V

L

E

A

K

F

L

K

V

S

E

L

I

Y

A

Y

E

R

R

V

V

-

G

-

L

-

T

-

P

-

E

-

D

-

A

I

I

A

A

A

V

G

G

D
|
D

S

G

Y

A

N

N

D

D

T

L

T

G

M

M

L

I

G

Q

E

L

A

A

D

G

A

T

G

G

I

V

L

A

F

L

H

H

A

A

P

K

D

-

N

-

V

-

I

-

R

-

E

-

F

-

P

-

Q

-

F

-

P

P

A

A

V

V

H

A

S

A

F

Q

A

A

E

K

L

M

K

R

binding magnesium ion: D243 (= D152)

Sites not aligning to the query:

binding magnesium ion: 86, 88

Query Sequence

>Pf1N1B4_3813 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3813
VEIACLDLEGVLVPEIWIAFAEKTGIDSLRATTRDIPDYDVLMKQRLRILDEHGLKLSDI
QEVIATLKPLEGASEFVDWLRERFQVVILSDTFYEFSQPLMRQLGFPTLLCHRLITDDAG
RVTGYQLRQKDPKRQSVLAFKSLYYRVIAAGDSYNDTTMLGEADAGILFHAPDNVIREFP
QFPAVHSFAELKQEFIKASNRALSL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory