SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_410 L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2) to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P04983 Ribose import ATP-binding protein RbsA; EC 7.5.2.7 from Escherichia coli (strain K12) (see paper)
46% identity, 95% coverage: 16:504/514 of query aligns to 5:492/501 of P04983

query
sites
P04983

L

L

R

Q

F

L

N

K

G

G

I

I

G

D

K

K

T

A

F

F

P

P

G

G

V

V

K

K

A

A

L

L

D

S

G

G

I

A

S

A

F

L

V

N

A

V

H

Y

P

P

G

G

Q

R

V

V

H

M

A

A

L

L

M

V

G

G

E

E

N

N

G

G

A

A

G

G

K
|
K

S

S

T

T

L

M

K

K

I

V

L

L

G

T

G

G

A

I

Y

Y

T

T

P

R

S

D

S

A

G

G

D

T

L

L

Q

L

I

W

G

L

E

G

Q

K

K

E

R

T

I

T

F

F

K

T

S

G

T

P

A

K

D

S

S

S

I

Q

G

E

S

A

G

G

V

I

A

G

V

I

I

I

H

H

Q

Q

E

E

L

L

H

N

L

L

V

I

P

P

E

Q

M

L

T

T

V

I

A

A

E

E

N

N

L

I

F

F

L

L

G

G

-

R

H

E

L

F

P

V

A

N

S

R

F

F

G

G

L

K

I

I

N

D

R

W

G

K

V

T

L

M

R

Y

Q

A

Q

E

A

A

L

D

A

K

C

L

L

L

K

A

G

K

L

L

A

N

D

L

E

R

I

F

D

K

P

S

Q

D

E

K

K

L

V

V

G

G

R

D

L

L

S

S

L

I

G

G

Q

D

R

Q

Q

Q

L

M

V

V

E

E

I

I

A

A

K

K

A

V

L

L

S

S

R

F

G

E

A

S

H

K

V

V

I

I

A

I

F

M

D

D

E

E

P

P

T

T

S

D

S

A

L

L

S

T

A

D

R

T

E

E

I

T

D

E

R

S

L

L

M

F

A

R

I

V

I

I

G

R

R

E

L

L

R

K

D

S

E

Q

G

G

K

R

V

G

V

I

L

V

Y

Y

V

I

S

S

H

H

R

R

M

M

E

K

E

E

V

I

F

F

R

E

I

I

C

C

N

D

A

D

V

V

T

T

V

V

F

F

K

R

D

D

G

G

R

Q

F

F

V

I

R

A

T

E

F

R

E

E

D

-

M

V

S

A

A

S

L

L

T

T

H

E

D

D

Q

S

L

L

V

I

T

E

C

M

M

M

V

V

G

G

R

R

D

K

I

L

Q

E

D

D

I

Q

Y

Y

D

P

Y

H

R

L

S

D

R

K

P

A

R

P

G

G

A

D

V

I

A

R

L

L

K

K

V

V

D

D

G

N

L

L

L

C

G

G

P

P

G

G

L

V

R

N

E

D

P

-

V

V

S

S

F

F

E

T

A

L

H

R

K

K

G

G

E

E

I

I

L

L

G

G

L

V

F

S

G

G

L

L

V

M

G

G

A

A

G

G

R

R

T

T

E

E

L

L

F

M

R

K

M

V

L

L

S

Y

G

G

L

A

T

L

R

P

N

R

T

T

A

S

G

G

R

Y

L

V

E

T

L

L

R

D

G

G

R

H

E

E

L

V

K

V

L

T

H

R

S

S

P

P

R

Q

D

D

A

G

I

L

A

A

A

N

G

G

I

I

L

V

L

Y

C

I

P

S

E

E

D

D

R

R

K

K

K

R

E

D

G

G

I

L

L

V

P

L

L

G

A

M

S

S

V

V

A

K

E

E

N

N

I

M

N

S

I

L

S

T

A

A

R

L

G

R

A

Y

H

F

S

S

T

R

F

A

G

G

C

G

L

S

L

L

R

K

G

H

L

A

W

D

E

E

K

Q

D

Q

N

A

A

V

D

S

K

D

Q

F

I

I

K

R

A

L

L

F

K

N

V

V

K

K

T

T

P

P

N

S

A

M

A

E

Q

Q

K

A

I

I

M

G

Y

L

L

L

S

S

G

G

N

N

Q

Q

K

K

A

V

I

A

L

I

G

A

R

R

W

G

L

L

S

M

M

T

P

R

M

P

K

K

V

V

L

L

L

I

L

L

D

D

E

E

P

P

T

T

R

R

G

G

I

V

D

D

I

V

G

G

A

A

K

K

A

K

E

E

I

I

Y

Y

Q

Q

I

L

I

I

H

N

N

Q

L

F

A

K

A

A

S

D

G

G

I

L

A

S

V

I

I

I

V

L

V

V

S

S

D

E

L

M

M

P

E

E

V

V

M

L

G

G

I

M

S

S

D

D

R

R

I

I

L

I

V

V

L

M

C

H

E

E

G

G

A

H

M

L

R

S

G

G

E

E

L

F

T

T

R

R

E

E

Q

Q

A

A

N

T

E

Q

S

E

N

V

L

L

L

M

Q

A

L

A

A

A

L

V

K43 (= K54) mutation to R: Loss of transport.

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 40% coverage: 31:238/514 of query aligns to 25:232/233 of P75957

query
sites
P75957

L

L

D

H

G

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G
|
G

E

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

T

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

-

P

-

M

-

S

K

K

R

L

I

S

F

S

K

A

S

A

T

K

A

A

D

E

S

L

I

R

G

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q

Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

S

L

F

I

G

G

L

K

I

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

A

E

C

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D

R

P

A

Q

N

E

H

K

R

V

P

G

S

R

E

L

L

S

S

L

G

G

G

Q

E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H

H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

F

L

V

T

R

A

T

E

F

L

E

S

D

L

M

M

S

G

A

A

G42 (= G48) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
33% identity, 39% coverage: 31:230/514 of query aligns to 22:221/226 of 7mdyC

query
sites
7mdyC

L

L

D

H

G

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E

S

N

S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

T

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

-

P

-

M

-

S

K

K

R

L

I

S

F

S

K

A

S

A

T

K

A

A

D

E

S

L

I

R

G

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q
|
Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

S

L

F

I

G

G

L

K

I

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

A

E

C

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D

R

P

A

Q

N

E
x
H

K

R

V

P

G

S

R
x
E

L

L

S
|
S

L
x
G

G
|
G

Q

E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E
|
E

P

P

T

T

S

G

S
x
N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H
|
H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

F

L

V

T

binding adp orthovanadate: G42 (= G51), S43 (≠ A52), G44 (= G53), K45 (= K54), S46 (= S55), T47 (= T56), Q91 (= Q97), H138 (≠ E147), E142 (≠ R151), S144 (= S153), G145 (≠ L154), G146 (= G155), E168 (= E177), N172 (≠ S181), H201 (= H209)
binding magnesium ion: S46 (= S55), Q91 (= Q97)

Sites not aligning to the query:

binding adp orthovanadate: 12

7arlD Lolcde in complex with lipoprotein and adp (see paper)
33% identity, 39% coverage: 31:230/514 of query aligns to 22:221/222 of 7arlD

query
sites
7arlD

L

L

D

H

G

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E

S

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

T

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

-

P

-

M

-

S

K

K

R

L

I

S

F

S

K

A

S

A

T

K

A

A

D

E

S

L

I

R

G

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q

Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

S

L

F

I

G

G

L

K

I

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

A

E

C

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D

R

P

A

Q

N

E

H

K

R

V

P

G

S

R

E

L

L

S

S

L

G

G

G

Q

E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H

H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

F

L

V

T

binding adenosine-5'-diphosphate: G42 (= G51), G44 (= G53), K45 (= K54), S46 (= S55)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
33% identity, 42% coverage: 16:231/514 of query aligns to 5:223/648 of P75831

query
sites
P75831

L

L

R

E

F

L

N

K

G

D

I

I

G

R

K

R

T

S

F

Y

P

P

G

A

-

G

-

D

-

E

-

Q

V

V

K

E

A

V

L

L

D

K

G

G

I

I

S

S

F

L

V

D

A

I

H

Y

P

A

G

G

Q

E

V

M

H

V

A

A

L

I

M

V

G

G

E

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

L

M

K

N

I

I

L

L

G

G

G

C

A

L

Y

D

T

K

P

A

S

T

S

S

G

G

D

T

L

Y

Q

R

I

V

G

A

E

G

Q

Q

K

D

R

-

I

V

F

A

K

T

S

L

T

D

A

A

D

D

S

A

I

L

G

A

S

Q

-

L

-

R

-

E

G

H

V

F

A

G

V

F

I

I

H

F

Q

Q

E

R

L

Y

H

H

L

L

V

L

P

S

E

H

M

L

T

T

V

A

A

E

E

Q

N

N

L

V

F

-

L

-

G

-

H

E

L

V

P

P

A

A

S

V

F

Y

G

A

L

G

I

L

N

E

R

R

G

K

-

Q

-

R

V

L

L

L

R

R

Q

A

Q

Q

A

E

L

L

A

L

C

Q

L

R

K

L

G

G

L

L

A

E

D

D

E

R

I

T

D

E

P

Y

Q

Y

E

P

K

-

V

-

G

A

R

Q

L

L

S

S

L

G

G

G

Q

Q

R

Q

Q

Q

L

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

R

N

G

G

A

G

H

Q

V

V

I

I

A

L

F

A

D
|
D

E

E

P

P

T

T

S

G

S

A

L

L

S

D

A

S

R

H

E

S

I

G

D

E

R

E

L

V

M

M

A

A

I

I

I

L

G

H

R

Q

L

L

R

R

D

D

E

R

G

G

K

H

V

T

V

V

L

I

Y

I

V

V

S

T

H

H

R

D

M

-

E

P

E

Q

V

V

F

A

R

A

I

Q

C

A

N

E

A

R

V

V

T

I

V

E

F

I

K

R

D

D

G

G

R

E

F

I

V

V

R

R

K47 (= K54) mutation to L: Lack of activity.
D169 (= D176) mutation to N: Lack of activity.

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
32% identity, 39% coverage: 31:230/514 of query aligns to 24:223/229 of 7v8iD

query
sites
7v8iD

L

L

D

H

G

N

I

V

S

S

F

F

V

S

A

V

H

G

P

E

G

G

Q

E

V

M

H

M

A

A

L

I

M

V

G

G

E

S

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

H

I

L

L

L

G

G

A

L

Y

D

T

T

P

P

S

T

S

S

G

G

D

D

L

V

Q

I

I

F

G

N

E

G

Q

Q

-

P

-

M

-

S

K

K

R

L

I

S

F

S

K

A

S

A

T

K

A

A

D

E

S

L

I

R

G

N

S

Q

G

K

V

L

A

G

V

F

I

I

H

Y

Q
|
Q

E

F

L

H

H

H

L

L

V

L

P

P

E

D

M

F

T

T

V

A

A

L

E

E

N

N

L

V

F

-

L

-

G

-

H

A

L

M

P

P

A

L

S

L

F

I

G

G

L

K

I

K

N

K

R

P

G

A

V

E

L

I

R

N

Q

S

Q

R

A

A

L

L

A

E

C

M

L

L

K

K

G

A

L

V

A

G

D

L

E

D

I

H

D
x
R

P

A

Q

N

E
x
H

K

R

V

P

G

S

R
x
E

L

L

S
|
S

L

G

G
|
G

Q
x
E

R

R

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

N

G

N

A

P

H

R

V

L

I

V

A

L

F

A

D

D

E

Q

P

P

T

T

S

G

S

N

L

L

S

D

A

A

R

R

E

N

I

A

D

D

R

S

L

I

M

F

A

Q

I

L

G

G

R

E

L

L

-

N

R

R

D

L

E

Q

G

G

K

T

V

A

V

F

L

L

Y

V

V

V

S

T

H
|
H

R

D

M

L

E

Q

E

L

V

A

F

K

R

R

I

M

C

S

N

R

A

Q

V

L

T

E

V

M

F

-

K

R

D

D

G

G

R

R

F

L

V

T

binding phosphoaminophosphonic acid-adenylate ester: S43 (≠ N50), G44 (= G51), G46 (= G53), K47 (= K54), S48 (= S55), T49 (= T56), Q93 (= Q97), R137 (≠ D144), H140 (≠ E147), E144 (≠ R151), S146 (= S153), G148 (= G155), E149 (≠ Q156), H203 (= H209)
binding magnesium ion: S48 (= S55), Q93 (= Q97)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 23

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
30% identity, 42% coverage: 16:231/514 of query aligns to 2:225/232 of 1f3oA

query
sites
1f3oA

L

I

R

K

F

L

N

K

G

N

I

V

G

T

K

K

T

T

F
x
Y

P

K

G

M

-

G

-

E

-

I

V

I

K

Y

A

A

L

L

D

K

G

N

I

V

S

N

F

L

V

N

A

I

H

K

P

E

G

G

Q

E

V

F

H

V

A

S

L

I

M

M

G

G

E

P

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

K

N

I

I

L

I

G

G

G

C

A

L

Y

D

T

K

P

P

S

T

S

E

G

G

D

E

L

V

Q

Y

I

I

G

D

E

N

Q

I

K

K

-

T

-

N

-

D

-

L

-

D

R

E

I

L

F

T

K

K

S

I

T

R

A

R

D

D

S

K

I

I

G

G

S

-

G

-

V

-

A

-

V

F

I

V

H

F

Q

Q

E

Q

L

F

H

N

L

L

V

I

P

P

E

L

M

L

T

T

V

A

A

L

E

E

N

N

L

V

F

E

L

L

G

P

H

L

L

I

P

F

A

K

S

Y

F

R

G

G

L

A

I

M

N

S

R

G

G

E

V

E

L

R

R

R

Q

K

Q

R

A

A

L

L

A

E

C

C

L

L

K

K

-

M

G

A

L

E

A

L

D

E

E

E

I

R

D

F

P

A

Q

N

E

H

K

K

V

P

G

N

R

Q

L

L

S

S

L

G

G

G

Q

Q

R

Q

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

R

N

G

N

A

P

H

P

V

I

I

I

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

A

L

L

S

D

A

S

R

K

E

T

I

G

D

E

R

K

L

I

M

M

A

Q

I

L

G

K

R

K

L

L

R

N

D

E

E

E

-

D

G

G

K

K

V

T

V

V

L

V

Y

V

V

V

S

T

H

H

R

D

M

I

E

-

E

N

V

V

F

A

R

R

I

F

C

G

N

E

A

R

V

I

T

I

V

Y

F

L

K

K

D

D

G

G

R

E

F

V

V

E

R

R

binding adenosine-5'-diphosphate: Y11 (≠ F25), G41 (= G51), G43 (= G53), K44 (= K54), S45 (= S55), T46 (= T56)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
30% identity, 41% coverage: 19:230/514 of query aligns to 5:215/240 of 4ymuJ

query
sites
4ymuJ

N

N

G

D

I

V

G

Y

K

K

T

N

F
|
F

P

G

G

S

V

L

K

E

A
x
V

L

L

D

K

G

G

I

V

S

T

F

L

V

K

A

V

H

N

P

K

G

G

Q

E

V

V

H

V

A

V

L

I

M

I

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

C

L

I

G

N

G

L

A

L

Y

E

T

E

P

P

S

T

S

K

G

G

D

E

L

V

Q

F

I

I

-

D

G

G

E

V

Q

K

K

I

R

N

I

N

F

G

K

K

S

V

T

N

A

I

D

N

S

K

I

V

G

R

S

Q

G

K

V

V

A

G

V

M

I

V

H

F

Q

Q

E

H

L

F

H

N

L

L

V

F

P

P

E

H

M

L

T

T

V

A

A

I

E

E

N

N

L

I

F

T

L

L

G

A

H

-

L

-

P

P

A

V

S

K

F

V

G

K

L

K

I

M

N

N

R

K

G

K

V

E

L

A

R

E

Q

E

Q

L

A

A

L

V

A

D

C

L

L

L

K

A

-

K

-

V

G

G

L

L

A

L

D

D

E

K

I

K

D

D

P

-

Q

Q

E

Y

K

P

V

I

G

-

R

K

L

L

S

S

L

G

G

G

Q

Q

R

K

Q

Q

L

R

V

L

E

A

I

I

A

A

K

R

A

A

L

L

S

A

R

M

G

Q

A

P

H

E

V

V

I

M

A

L

F

F

D

D

E
|
E

P

P

T

T

S

S

S

A

L

L

S

D

A

P

R

E

E

M

I

V

D

K

R

E

L

V

M

L

A

N

I

V

I

M

G

K

R

Q

L

L

R

A

D

N

E

E

G

G

K

M

V

T

V

M

L

V

Y

V

V

V

S

T

H
|
H

R

E

M

M

E

G

E

F

V

A

F

R

R

E

I

V

C

G

N

D

A

R

V

V

T

I

V

F

F

M

K

D

D

D

G

G

R

V

F

I

V

V

binding adenosine-5'-triphosphate: F11 (= F25), V16 (≠ A30), S36 (≠ N50), G37 (= G51), S38 (≠ A52), G39 (= G53), K40 (= K54), S41 (= S55), T42 (= T56), E162 (= E177), H194 (= H209)
binding magnesium ion: S41 (= S55), E162 (= E177)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
30% identity, 42% coverage: 16:231/514 of query aligns to 2:225/230 of 1l2tA

query
sites
1l2tA

L

I

R

K

F

L

N

K

G

N

I

V

G

T

K

K

T

T

F
x
Y

P

K

G

M

-

G

-

E

-

I

V

I

K

Y

A

A

L

L

D

K

G

N

I

V

S

N

F

L

V

N

A

I

H

K

P

E

G

G

Q

E

V

F

H

V

A

S

L

I

M

M

G

G

E

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

K

N

I

I

L

I

G

G

G

C

A

L

Y

D

T

K

P

P

S

T

S

E

G

G

D

E

L

V

Q

Y

I

I

G

D

E

N

Q

I

K

K

-

T

-

N

-

D

-

L

-

D

R

E

I

L

F

T

K

K

S

I

T

R

A

R

D

D

S

K

I

I

G

G

S

-

G

-

V

-

A

-

V

F

I

V

H

F

Q

Q

E

Q

L

F

H

N

L

L

V

I

P

P

E

L

M

L

T

T

V

A

A

L

E

E

N

N

L

V

F

E

L

L

G

P

H

L

L

I

P

F

A

K

S

Y

F

R

G

G

L

A

I

M

N

S

R

G

G

E

V

E

L

R

R

R

Q

K

Q

R

A

A

L

L

A

E

C

C

L

L

K

K

-

M

G

A

L

E

A

L

D

E

E

E

I

R

D
x
F

P

A

Q

N

E

H

K

K

V

P

G

N

R
x
Q

L

L

S
|
S

L

G

G
|
G

Q
|
Q

R

Q

Q

Q

L

R

V

V

E

A

I

I

A

A

K

R

A

A

L

L

S

A

R

N

G

N

A

P

H

P

V

I

I

I

A

L

F

A

D

D

E

Q

P

P

T

T

S

G

S

A

L

L

S

D

A

S

R

K

E

T

I

G

D

E

R

K

L

I

M

M

A

Q

I

L

G

K

R

K

L

L

R

N

D

E

E

E

-

D

G

G

K

K

V

T

V

V

L

V

Y

V

V

V

S

T

H
|
H

R

D

M

I

E

-

E

N

V

V

F

A

R

R

I

F

C

G

N

E

A

R

V

I

T

I

V

Y

F

L

K

K

D

D

G

G

R

E

F

V

V

E

R

R

binding adenosine-5'-triphosphate: Y11 (≠ F25), S40 (≠ N50), G41 (= G51), S42 (≠ A52), G43 (= G53), K44 (= K54), S45 (= S55), T46 (= T56), F138 (≠ D144), Q145 (≠ R151), S147 (= S153), G149 (= G155), Q150 (= Q156), H204 (= H209)

P21439 Phosphatidylcholine translocator ABCB4; ATP-binding cassette sub-family B member 4; Multidrug resistance protein 3; P-glycoprotein 3; EC 7.6.2.1 from Homo sapiens (Human) (see 22 papers)
31% identity, 43% coverage: 12:230/514 of query aligns to 390:609/1286 of P21439

query
sites
P21439

I

I

G

K

G

G

S

N

L

L

R

E

F

F

N

N

G

D

I

V

G

H

K

F

T

S

F
x
Y

P

P

-

S

-
x
R

-

A

G

N

V

V

K

K

A

I

L

L

D

K

G

G

I

L

S

N

F

L

V

K

A

V

H

Q

P

S

G

G

Q

Q

V

T

H

V

A

A

L

L

M

V

G

G

E

S

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

T

L

V

K

Q

I

L

L

I

G

Q

G

R

A

L

Y

Y

T

D

P

P

S

D

S
x
E

G

G

D

T

L

I

Q

N

I

I

-

D

G

G

E

Q

Q
x
D

K

I

R

R

I

N

F

F

K

-

S

-

T

N

A

V

D

N

S

Y

I

L

G

R

S

E

G

I

V

I

A

G

V

V

I

V

H

S

Q
|
Q

E

E

L
x
P

H

V

L
|
L

V

F

P

-

E

S

M

T

T

T

V

I

A

A

E

E

N

N

L

I

F

C

L

Y

G

G

H

R

L

G

P

N

A

V

S

T

F

M

G

D

L

E

I

I

N

K

R

K

G

A

V

V

L

K

R

E

Q

A

Q

N

A
|
A

L

Y

A

E

C

F

L

I

K

M

G

K

L

L

A

P

D

Q

E

K

I

F

D

D

P

T

Q

L

-

V

-

G

E
|
E

K

R

V

G

G

A

R

Q

L

L

S

S

L
x
G

G
|
G

Q

Q

R

K

Q

Q

L

R

V
x
I

E

A

I

I

A

A

K

R

A
|
A

L

L

S

V

R

R

G

N

A

P

H

K

V

I

I

L

A

L

F

L

D

D

E
|
E

P

A

T

T

S

S

S

A

L

L

S

D

A

T

R

E

E

S

I

E

D

A

R

E

L

V

M

Q

A

A

I

A

I

L

G

D

R

K

L

A

R

R

D

-

E

E

G

G

K

R

V

T

L

I

Y

V

V

I

S

A

H
|
H

R
|
R

M

L

E

S

E

T

V

V

F

-

R

R

I

N

C

A

N

D

A

V

V

I

T

A

V

G

F

F

K

E

D

D

G

G

R

V

F

I

V

V

Y403 (≠ F25) to H: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol; dbSNP:rs121918443
R406 (vs. gap) binding ; to Q: found in patients with cholangitis; uncertain significance; dbSNP:rs763807769
GCGKST 432:437 (≠ GAGKST 51:56) binding
K435 (= K54) mutation to M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
E450 (≠ S69) to G: in dbSNP:rs1189003716
D459 (≠ Q77) to H: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression
Q477 (= Q97) binding
P479 (≠ L99) to L: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs748657435
L481 (= L101) to R: in PFIC3; does not alter cytoplasmic and cell membrane location; inhibits efflux activity for PC and cholesterol
A511 (= A132) to T: in PFIC3 and GBD1; dbSNP:rs1257887155
E528 (= E147) to D: in GBD1; uncertain significance; moderate decrease of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs8187797
G535 (≠ L154) to D: in PFIC3; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
G536 (= G155) binding ; to R: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location
I541 (≠ V160) to F: in PFIC3 and GBD1; dbSNP:rs66904256
A546 (= A165) to D: in ICP3; disruption of protein trafficking with subsequent lack of functional protein at the cell surface; dbSNP:rs121918441
E558 (= E177) mutation to Q: Loss of floppase activity. Strongly reduce the ATPase activity.
H589 (= H209) to T: in GBD1; requires 2 nucleotide substitutions
R590 (= R210) to Q: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs45575636

Sites not aligning to the query:

34 modified: Phosphothreonine; T → M: in GBD1; reduces efflux activity for PC in a phosphorylation-dependent manner; dbSNP:rs142794414; T→D: Does not inhibit efflux activity for PC.
44 T→A: Reduces efflux activity for PC. Does not alter apical membrane location.
47 R → G: in GBD1; partly retained intracellularly; reduces efflux activity for PC in a phosphorylation-dependent manner; R → Q: found in patients with cholangitis; uncertain significance; dbSNP:rs372685632
49 S→A: Reduces efflux activity for PC. Does not alter apical membrane location.
68 G → R: in PFIC3; retained in the reticulum endoplasmic; greatly reduced expression; dbSNP:rs1343667900
73 L → V: in PFIC3 and GBD1; dbSNP:rs8187788
87 natural variant: D -> E
95 P → S: in dbSNP:rs377268767
175 T → A: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs58238559
201 T → M: in PFIC3; greatly reduced expression; alters efflux activity for PC; dbSNP:rs753318087
238 L → V: in dbSNP:rs45596335
263 I → V: in dbSNP:rs45547936
286 A → V: in PFIC3 and GBD1; does not alter plasma membrane location; inhibits efflux activity for PC; dbSNP:rs765478923
320 S → F: in ICP3, GBD1 and PFIC3; uncertain significance; does not alter plasma membrane location; does not inhibit efflux activity for PC; dbSNP:rs72552778
367 I → V: in dbSNP:rs1168923653
651 T → N: in dbSNP:rs45476795
652 R → G: in dbSNP:rs2230028
726 P → L: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs141677867
742 natural variant: G -> S
764 I → L: in a heterozygous patient with risperidone-induced cholestasis
775 T → M: found in patients with cholangitis; uncertain significance; dbSNP:rs148052192
788 R → Q: in GBD1; benign; dbSNP:rs8187801
934 A → T: found in patients with gallbladder and cholestasis; uncertain significance; dbSNP:rs61730509
953 A→D: Accumulates predominantly in intracellular compartments with only a small fraction at the plasma membrane and inhibits partially the efflux activity for PC.
964 V → T: found in patients with cholangitis; uncertain significance; requires 2 nucleotide substitutions
978 S → P: in PFIC3; alters efflux activity for PC; dbSNP:rs1051861187
985 V→M: Significantly reduces phosphatidylcholine floppase activity; when associated with Q-989 and V-990.
989 H→Q: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and V-990.
990 A→V: Significantly reduces phosphatidylcholine floppase activity; when associated with M-985 and Q-989.
1046 binding
1071:1077 binding
1075 K→M: Inhibits efflux activity for PC and cholesterol, but does not alter glycosylation and surface expression in the presence of taurocholate.
1082 L → Q: in a heterozygous patient with amoxicillin/clavulanic acid-induced cholestasis; dbSNP:rs1214110864
1124 binding
1125 E → K: in PFIC3; alters efflux activity for PC
1168 P → S: in GBD1; reduced phosphatidylcholine transporter activity; does not alter plasma membrane location; dbSNP:rs121918442
1183 S → L: in GBD1; severely reduced phosphatidylcholine transporter activity; does not alter plasma membrane location
1184:1186 binding
1185 G → S: in GBD1; loss of phosphatidylcholine transporter activity; does not alter plasma membrane location

6s7pA Nucleotide bound abcb4 (see paper)
30% identity, 43% coverage: 12:230/514 of query aligns to 329:548/1128 of 6s7pA

query
sites
6s7pA

I

I

G

K

G

G

S

N

L

L

R

E

F

F

N

N

G

D

I

V

G

H

K

F

T

S

F
x
Y

P

P

-
x
S

-
x
R

-

A

G

N

V

V

K

K

A

I

L

L

D

K

G

G

I

L

S

N

F

L

V

K

A

V

H

Q

P

S

G

G

Q

Q

V

T

H

V

A

A

L

L

M

V

G

G

E

S

N

S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T

T

L

T

L

V

K

Q

I

L

L

I

G

Q

G

R

A

L

Y

Y

T

D

P

P

S

D

S

E

G

G

D

T

L

I

Q

N

I

I

-

D

G

G

E

Q

Q

D

K

I

R

R

I

N

F

F

K

-

S

-

T

N

A

V

D

N

S

Y

I

L

G

R

S

E

G

I

V

I

A

G

V

V

I

V

H

S

Q
|
Q

E

E

L

P

H

V

L

L

V

F

P

-

E

S

M

T

T

T

V

I

A

A

E

E

N

N

L

I

F

C

L

Y

G

G

H

R

L

G

P

N

A

V

S

T

F

M

G

D

L

E

I

I

N

K

R

K

G

A

V

V

L

K

R

E

Q

A

Q

N

A

A

L

Y

A

E

C

F

L

I

K

M

G

K

L

L

A

P

D

Q

E

K

I

F

D

D

P

T

Q

L

-

V

-

G

E

E

K

R

V

G

G

A

R
x
Q

L

L

S
|
S

L

G

G
|
G

Q
|
Q

R

K

Q

Q

L

R

V

I

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

R

G

N

A

P

H

K

V

I

I

L

A

L

F

L

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

S

D

A

T

R

E

E

S

I

E

D

A

R

E

L

V

M

Q

A

A

I

A

I

L

G

D

R

K

L

A

R

R

D

-

E

E

G

G

K

R

V

T

L

I

Y

V

V

I

S

A

H

H

R

R

M

L

E

S

E

T

V

V

F

-

R

R

I

N

C

A

N

D

A

V

V

I

T

A

V

G

F

F

K

E

D

D

G

G

R

V

F

I

V

V

binding adenosine-5'-triphosphate: Y342 (≠ F25), S344 (vs. gap), R345 (vs. gap), G371 (= G51), G373 (= G53), K374 (= K54), S375 (= S55), Q416 (= Q97), Q471 (≠ R151), S473 (= S153), G475 (= G155), Q476 (= Q156)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 920, 922, 923, 948, 949, 951, 952, 953, 954, 994, 1033, 1053, 1054, 1056
binding cholesterol: 25, 32, 35, 65, 824, 834, 834, 838
binding magnesium ion: 953, 994

7niwA Nanodisc reconstituted human abcb4 in complex with 4b1-fab (posaconazole-bound, inward-open conformation) (see paper)
30% identity, 43% coverage: 12:230/514 of query aligns to 339:558/1140 of 7niwA

query
sites
7niwA

I

I

G

K

G

G

S

N

L

L

R

E

F

F

N

N

G

D

I

V

G

H

K

F

T

S

F

Y

P

P

-

S

-

R

-

A

G

N

V

V

K

K

A

I

L

L

D

K

G

G

I

L

S

N

F

L

V

K

A

V

H

Q

P

S

G

G

Q

Q

V

T

H

V

A

A

L

L

M

V

G

G

E

S

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

T

L

V

K

Q

I

L

L

I

G

Q

G

R

A

L

Y

Y

T

D

P

P

S

D

S

E

G

G

D

T

L

I

Q

N

I

I

-

D

G

G

E

Q

Q

D

K

I

R

R

I

N

F

F

K

N

S

-

T

-

A

V

D

N

S

Y

I

L

G

R

S

E

G

I

V

I

A

G

V

V

I

V

H

S

Q

Q

E

E

L

P

H

V

L

L

V

F

P

-

E

S

M

T

T

T

V

I

A

A

E

E

N

N

L

I

F

C

L

Y

G

G

H

R

L

G

P

N

A

V

S

T

F

M

G

D

L

E

I

I

N

K

R

K

G

A

V

V

L

K

R

E

Q

A

Q

N

A

A

L

Y

A

E

C

F

L

I

K

M

G

K

L

L

A

P

D

Q

E

K

I

F

D

D

P

T

Q

L

-

V

-

G

E

E

K

R

V

G

G

A

R

Q

L

L

S

S

L

G

G

G

Q

Q

R

K

Q

Q

L

R

V

I

E

A

I

I

A

A

K

R

A

A

L

L

S

V

R

R

G

N

A

P

H

K

V

I

I

L

A

L

F

L

D

D

E

Q

P

A

T

T

S

S

S

A

L

L

S

D

A

T

R

E

E

S

I

E

D

A

R

E

L

V

M

Q

A

A

I

A

I

L

G

D

R

K

L

A

R

R

D

-

E

E

G

G

K

R

V

T

L

I

Y

V

V

I

S

A

H

H

R

R

M

L

E

S

E

T

V

V

F

-

R

R

I

N

C

A

N

D

A

V

V

I

T

A

V

G

F

F

K

E

D

D

G

G

R

V

F

I

V

V

Sites not aligning to the query:

binding cholesterol: 70, 152, 160, 266, 641, 645, 648, 839
binding 1,2-dilinoleoyl-sn-glycero-3-phosphocholine: 173, 183, 250, 254, 257, 261, 286, 294, 298, 617, 882
binding posaconazole: 250, 291, 294, 658, 661, 726, 841, 871, 874, 878, 882

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
30% identity, 46% coverage: 14:250/514 of query aligns to 2:235/371 of P68187

query
sites
P68187

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F

W

P

G

G

E

V

V

K

V

A

V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H
x
A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N
x
K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A
x
V

-

N

-

Q

L
x
V

A

A

C
x
E

L

V

K

L

G

Q

L

L

A
|
A

D

H

E

L

I

L

D

D

P

R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S

S

L

G

G
|
G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D
|
D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q
x
R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

A85 (≠ H100) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ N124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ A132) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L133) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ C135) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (= A140) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G155) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D176) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ Q243) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
30% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puyA

query
sites
3puyA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F
x
W

P

G

G

E

V

V

K

V

A

V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A

V

-

N

-

Q

L

V

A

A

C

E

L

V

K

L

G

Q

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L

L

S
|
S

L

G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E
|
E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F25), S37 (≠ N50), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), Q81 (= Q97), R128 (≠ P145), A132 (≠ V149), S134 (= S153), G136 (= G155), Q137 (= Q156), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
30% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puxA

query
sites
3puxA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F
x
W

P

G

G

E

V

V

K

V

A

V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A

V

-

N

-

Q

L

V

A

A

C

E

L

V

K

L

G

Q

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S
|
S

L

G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), R128 (≠ P145), S134 (= S153), Q137 (= Q156)
binding beryllium trifluoride ion: S37 (≠ N50), G38 (= G51), K41 (= K54), Q81 (= Q97), S134 (= S153), G136 (= G155), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
30% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puwA

query
sites
3puwA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F
x
W

P

G

G

E

V

V

K

V

A
x
V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A

V

-

N

-

Q

L

V

A

A

C

E

L

V

K

L

G

Q

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L

L

S
|
S

L
x
G

G
|
G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E
|
E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H
|
H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), R128 (≠ P145), A132 (≠ V149), S134 (= S153), Q137 (= Q156)
binding tetrafluoroaluminate ion: S37 (≠ N50), G38 (= G51), K41 (= K54), Q81 (= Q97), S134 (= S153), G135 (≠ L154), G136 (= G155), E158 (= E177), H191 (= H209)
binding magnesium ion: S42 (= S55), Q81 (= Q97)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
30% identity, 46% coverage: 14:250/514 of query aligns to 1:234/371 of 3puvA

query
sites
3puvA

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F
x
W

P

G

G

E

V

V

K

V

A
x
V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q
|
Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A

V

-

N

-

Q

L

V

A

A

C

E

L

V

K

L

G

Q

L

L

A

A

D

H

E

L

I

L

D

D

P
x
R

Q

K

E

P

K

K

V
x
A

G

-

R

-

L

L

S
|
S

L

G

G

G

Q
|
Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), G38 (= G51), C39 (≠ A52), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56), R128 (≠ P145), A132 (≠ V149), S134 (= S153), Q137 (= Q156)
binding magnesium ion: S42 (= S55), Q81 (= Q97)

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
30% identity, 46% coverage: 14:250/514 of query aligns to 1:234/374 of 2awnB

query
sites
2awnB

G

A

S

S

L

V

R

Q

F

L

N

Q

G

N

I

V

G

T

K

K

T

A

F
x
W

P

G

G

E

V

V

K

V

A
x
V

L

S

D

K

G

D

I

I

S

N

F

L

V

D

A

I

H

H

P

E

G

G

Q

E

V

F

H

V

A

V

L

F

M

V

G

G

E

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

K

R

I

M

L

I

G

A

G

G

A

L

Y

E

T

T

P

I

S

T

S

S

G

G

D

D

L

L

Q

F

I

I

G

G

E

E

Q

K

K

R

R

M

I

N

F

D

K

T

S

P

T

P

A

A

D

E

S

-

I

-

G

-

S

R

G

G

V

V

A

G

V

M

I

V

H

F

Q

Q

E

S

L

Y

H

A

L

L

V

Y

P

P

E

H

M

L

T

S

V

V

A

A

E

E

N

N

L

M

F

-

L

-

G

-

H

-

L

-

P

-

A

-

S

S

F

F

G

G

L

L

-

K

-

L

-

A

-

G

I

A

N

K

R

K

G

E

V

V

L

I

R

N

Q

Q

Q

R

A

V

-

N

-

Q

L

V

A

A

C

E

L

V

K

L

G

Q

L

L

A

A

D

H

E

L

I

L

D

D

P

R

Q

K

E

P

K

K

V

A

G

-

R

-

L

L

S

S

L

G

G

G

Q

Q

R

R

Q

Q

L

R

V

V

E

A

I

I

A

G

K

R

A

T

L

L

S

V

R

A

G

E

A

P

H

S

V

V

I

F

A

L

F

L

D

D

E

E

P

P

T

L

S

S

S

N

L

L

S

D

A

A

R

A

E

L

I

R

D

V

R

Q

L

M

M

R

A

I

I

E

I

I

G

S

R

R

L

L

R

H

D

K

E

R

-

L

G

G

K

R

V

T

V

M

L

I

Y

Y

V

V

S

T

H

H

R

D

M

Q

E

V

E

E

V

A

F

M

R

T

I

L

C

A

N

D

A

K

V

I

T

V

V

V

F

L

K

D

D

A

G

G

R

R

F

V

V

A

R

Q

T

V

F

G

E

K

D

P

M

L

S

E

A

L

L

Y

T

H

H

Y

-

P

-

A

D

D

Q

R

L

F

V

V

T

A

C

G

M

F

V

I

G

G

binding adenosine-5'-diphosphate: W12 (≠ F25), V17 (≠ A30), S37 (≠ N50), G38 (= G51), G40 (= G53), K41 (= K54), S42 (= S55), T43 (= T56)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
29% identity, 42% coverage: 16:230/514 of query aligns to 5:222/650 of 5ws4A

query
sites
5ws4A

L

L

R

E

F

V

N

S

G

N

I

L

G

V

K

R

T

E

F

F

P

P

G

A

-

G

-

E

-

S

-

T

V

I

K

Q

A

I

L

L

D

K

G

G

I

I

S

D

F

L

V

T

A

I

H

Y

P

E

G

G

Q

E

V

L

H

V

A

A

L

I

M

V

G

G

E

Q

N
x
S

G
|
G

A

S

G

G

K

K

S
|
S

T

T

L

L

L

M

K

N

I

I

L

L

G

G

G

C

A

L

Y

D

T

R

P

P

S

T

S

S

G

G

D

S

L

Y

Q

K

I

V

G

N

E

G

Q

Q

K

E

R

-

I

T

F

G

K

K

S

L

T

E

A

P

D

D

S

Q

I

L

G

A

S

Q

-

L

-

R

-

E

G

Y

V

F

A

G

V

F

I

I

H

F

Q

Q

E

R

L

Y

H

H

L

L

V

L

P

G

E

D

M

L

T

S

V

A

A

E

E

G

N

N

L

V

F

-

L

-

G

-

H

E

L

V

P

P

A

A

S

V

F

Y

G

A

L

G

I

V

N

T

R

P

G

A

V

D

L

R

R

K

Q

Q

Q

R

A

A

L

T

A

A

C

L

L

L

K

T

G

E

L

L

A

G

D

L

E

G

I

T

D

K

P

T

Q

Q

E

N

K

R

V

P

G

S

R

Q

L

L

S

S

L

G

G

G

Q

Q

R

Q

Q

Q

L

R

V

V

E

S

I

I

A

A

K

R

A

A

L

L

S

M

R

N

G

G

A

G

H

D

V

V

I

I

A

L

F

A

D

D

E

E

P

P

T

T

S

G

S

A

L

L

S

D

A

S

R

H

E

S

I

G

D

V

R

E

L

V

M

M

A

R

I

I

I

L

G

R

R

E

L

L

R

N

D

A

E

A

G

G

K

H

V

T

V

I

L

I

Y

L

V

V

S

T

H

H

R

D

M

M

E

-

E

Q

V

V

F

A

R

K

I

N

C

A

N

T

A

R

V

I

T

I

V

E

F

I

K

S

D

D

G

G

R

E

F

I

V

I

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (≠ N50), G44 (= G51), S48 (= S55)

O65934 ABC transporter ATP-binding/permease protein Rv1747; EC 7.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
32% identity, 43% coverage: 9:227/514 of query aligns to 313:528/865 of O65934

query
sites
O65934

E

E

Q

T

R

R

I

V

G

G

S

-

L

L

R

D

F

V

N

R

G

G

I

V

G

T

K

W

T

T

F

I

P

D

G

G

V

D

K

K

A

T

L

L

-

L

D

D

G

G

I

I

S

S

F

L

V

T

A

A

H

R

P

P

G

G

Q

M

V

L

H

T

A

A

L

V

M

I

G

G

E

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

L

L

A

K

R

I

L

L

V

G

A

G

G

A

Y

Y

T

T

H

P

P

S

T

S

D

G

G

D

T

L

V

Q

T

I

F

G

-

E

E

Q

G

K

H

R

N

I

V

F

H

K

A

S

E

T

Y

A

A

D

-

S

S

I

L

G

R

S

S

G

R

V

I

A

G

V

M

I

V

H

P

Q

Q

E

D

L

D

H

V

L

V

V

H

P

G

E

Q

M

L

T

T

V

V

A

K

E

H

N

A

L

L

F

M

L

Y

G

A

-

A

-

E

-

L

H

R

L

L

P

P

A

P

S

D

F

T

G

T

L

K

I

D

N

D

R

R

G

T

V

Q

L

V

R

V

Q

A

Q

R

A

V

L

L

A

E

C

E

L

L

K

E

G

-

L

M

A

S

D

K

E

H

I

I

D

D

P

-

Q

-

E

T

K

R

V

V

G

D

R

K

L

L

S

S

L

G

G

G

Q

Q

R

R

Q

K

L

R

V

A

E

S

I

V

A

A

K

L

A

E

L

L

S

L

R

T

G

G

A

P

H

S

V

L

I

L

A

I

F

L

D

D

E
|
E

P

P

T

T

S

S

S

G

L

L

S

D

A

P

R

A

E

L

I

D

D

R

R

Q

L

V

M

M

A

T

I

M

I

L

G

R

R

Q

L

L

R

A

D

D

E

A

G

G

K

R

V

V

L

L

Y

V

V

V

S

T

H

H

R

S

M

L

E

-

E

T

V

Y

F

L

R

D

I

V

C

C

N

D

A

Q

V

V

T

L

V

L

F

L

K

A

D

P

G

G

E479 (= E177) mutation to Q: Loss of ATPase activity.

Sites not aligning to the query:

33 R→A: Strong decrease in phosphorylation.
47 S→A: Strong decrease in phosphorylation. Lack of interaction with PknF. Attenuates growth in macrophages.
69 N→A: Strong decrease in phosphorylation.
152 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-210.
210 modified: Phosphothreonine; T→A: Lack of phosphorylation. Attenuates growth in macrophages and in mice; when associated with A-152.
234 R→A: Strong decrease in phosphorylation.
248 S→A: Strong decrease in phosphorylation. Decreases interaction with PknF.
270 N→A: Strong decrease in phosphorylation.

Query Sequence

>Pf1N1B4_410 L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)
MHAHVQTQEQRIGGSLRFNGIGKTFPGVKALDGISFVAHPGQVHALMGENGAGKSTLLKI
LGGAYTPSSGDLQIGEQKRIFKSTADSIGSGVAVIHQELHLVPEMTVAENLFLGHLPASF
GLINRGVLRQQALACLKGLADEIDPQEKVGRLSLGQRQLVEIAKALSRGAHVIAFDEPTS
SLSAREIDRLMAIIGRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRFVRTFEDMSALT
HDQLVTCMVGRDIQDIYDYRSRPRGAVALKVDGLLGPGLREPVSFEAHKGEILGLFGLVG
AGRTELFRMLSGLTRNTAGRLELRGRELKLHSPRDAIAAGILLCPEDRKKEGILPLASVA
ENINISARGAHSTFGCLLRGLWEKDNADKQIKALKVKTPNAAQKIMYLSGGNQQKAILGR
WLSMPMKVLLLDEPTRGIDIGAKAEIYQIIHNLAASGIAVIVVSSDLMEVMGISDRILVL
CEGAMRGELTREQANESNLLQLALPRQRVADVAN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory