SitesBLAST
Comparing Pf1N1B4_4176 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4176 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8GIX7 Alcohol dehydrogenase; ADH; EC 1.1.1.1 from Moraxella sp. (strain TAE123) (see paper)
35% identity, 99% coverage: 1:325/327 of query aligns to 1:334/338 of Q8GIX7
- C38 (= C38) binding
- H61 (= H60) binding
- E62 (= E61) binding
- C92 (= C91) binding
- C95 (= C94) binding
- C98 (= C97) binding
- C106 (= C105) binding
- C148 (= C147) binding
4z6kA Alcohol dehydrogenase from the antarctic psychrophile moraxella sp. Tae 123
35% identity, 99% coverage: 1:325/327 of query aligns to 1:334/345 of 4z6kA
- active site: C38 (= C38), H39 (≠ R39), T40 (= T40), H43 (= H43), H61 (= H60), E62 (= E61), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), Q110 (≠ R109), C148 (= C147), T152 (≠ I151), R331 (≠ A322)
- binding zinc ion: C38 (= C38), H61 (= H60), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), C148 (= C147)
1lluA The ternary complex of pseudomonas aeruginosa alcohol dehydrogenase with its coenzyme and weak substrate (see paper)
36% identity, 99% coverage: 1:325/327 of query aligns to 6:339/341 of 1lluA
- active site: C43 (= C38), H44 (≠ R39), T45 (= T40), H48 (= H43), H66 (= H60), E67 (= E61), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), Q115 (≠ R109), C153 (= C147), T157 (≠ I151), R336 (≠ A322)
- binding 1,2-ethanediol: H44 (≠ R39), T45 (= T40), L47 (= L42), D53 (≠ E48), W92 (= W86), C153 (= C147)
- binding nicotinamide-adenine-dinucleotide: C43 (= C38), H44 (≠ R39), T45 (= T40), H48 (= H43), C153 (= C147), T157 (≠ I151), G179 (= G171), G180 (≠ A172), L181 (≠ A173), D200 (≠ T192), I201 (≠ R193), K205 (≠ E198), A243 (vs. gap), V244 (vs. gap), S245 (vs. gap), A248 (vs. gap), V265 (≠ A251), L267 (≠ I253), I290 (≠ A276), V291 (≠ N277), R336 (≠ A322)
- binding zinc ion: C43 (= C38), H66 (= H60), C100 (= C94), C103 (= C97), C111 (= C105), C153 (= C147)
3s2fE Crystal structure of furx nadh:furfural
36% identity, 99% coverage: 1:325/327 of query aligns to 3:336/340 of 3s2fE
- active site: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), Q112 (≠ R109), C150 (= C147), T154 (≠ I151), R333 (≠ A322)
- binding furfural: T42 (= T40), W51 (≠ L49), H63 (= H60), W89 (= W86), C150 (= C147), I287 (≠ A276)
- binding nicotinamide-adenine-dinucleotide: C40 (= C38), H41 (≠ R39), T42 (= T40), C150 (= C147), T154 (≠ I151), G174 (= G169), G176 (= G171), G177 (≠ A172), L178 (≠ A173), D197 (≠ T192), I198 (≠ P194), K202 (≠ E198), T239 (vs. gap), A240 (vs. gap), V241 (vs. gap), N262 (≠ A251), G263 (= G252), L264 (≠ I253), I287 (≠ A276), V288 (≠ N277), R333 (≠ A322)
- binding zinc ion: C40 (= C38), H63 (= H60), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), C150 (= C147)
3s2fA Crystal structure of furx nadh:furfural
36% identity, 99% coverage: 1:325/327 of query aligns to 3:336/340 of 3s2fA
- active site: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), Q112 (≠ R109), C150 (= C147), T154 (≠ I151), R333 (≠ A322)
- binding phosphorylisopropane: T42 (= T40), H63 (= H60), W89 (= W86), I287 (≠ A276)
- binding zinc ion: C40 (= C38), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), C150 (= C147)
3s2eE Crystal structure of furx nadh complex 1
36% identity, 99% coverage: 1:325/327 of query aligns to 3:336/340 of 3s2eE
- active site: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), Q112 (≠ R109), C150 (= C147), T154 (≠ I151), R333 (≠ A322)
- binding nicotinamide-adenine-dinucleotide: C40 (= C38), H41 (≠ R39), T42 (= T40), C150 (= C147), T154 (≠ I151), G176 (= G171), G177 (≠ A172), L178 (≠ A173), D197 (≠ T192), I198 (≠ P194), K202 (≠ E198), T239 (vs. gap), A240 (vs. gap), V241 (vs. gap), S242 (vs. gap), A245 (= A238), N262 (≠ A251), G263 (= G252), L264 (≠ I253), I287 (≠ A276), V288 (≠ N277)
- binding zinc ion: C40 (= C38), H63 (= H60), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), C150 (= C147)
3s2eA Crystal structure of furx nadh complex 1
36% identity, 99% coverage: 1:325/327 of query aligns to 3:336/340 of 3s2eA
- active site: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), Q112 (≠ R109), C150 (= C147), T154 (≠ I151), R333 (≠ A322)
- binding zinc ion: C40 (= C38), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), C150 (= C147)
P12311 Alcohol dehydrogenase; ADH-T; EC 1.1.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
36% identity, 99% coverage: 1:325/327 of query aligns to 1:334/337 of P12311
- C38 (= C38) mutation to S: No activity.
- T40 (= T40) mutation to A: No activity.; mutation to S: Little decrease in activity.
- H43 (= H43) mutation to A: No activity.; mutation to R: Higher level of activity at pH 9.
6iqdA Crystal structure of alcohol dehydrogenase from geobacillus stearothermophilus (see paper)
34% identity, 99% coverage: 1:325/327 of query aligns to 1:334/336 of 6iqdA
- active site: C38 (= C38), T40 (= T40), H43 (= H43), H61 (= H60), C148 (= C147)
- binding zinc ion: C38 (= C38), H61 (= H60), E62 (= E61), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), C148 (= C147)
1rjwA Crystal structure of NAD(+)-dependent alcohol dehydrogenase from bacillus stearothermophilus strain lld-r (see paper)
34% identity, 99% coverage: 1:325/327 of query aligns to 1:334/339 of 1rjwA
- active site: C38 (= C38), H39 (≠ R39), T40 (= T40), H43 (= H43), H61 (= H60), E62 (= E61), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), K110 (≠ R109), C148 (= C147), T152 (≠ I151), R331 (≠ A322)
- binding trifluoroethanol: T40 (= T40), C148 (= C147), I285 (≠ V275)
- binding zinc ion: C38 (= C38), H61 (= H60), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105)
3piiA Crystal structure of mutant of ht- alcohol dehydrogenase with substrate analogue butyramide
34% identity, 99% coverage: 1:325/327 of query aligns to 1:334/337 of 3piiA
- active site: C38 (= C38), H39 (≠ R39), T40 (= T40), H43 (= H43), H61 (= H60), E62 (= E61), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), K110 (≠ R109), C148 (= C147), T152 (≠ I151), R331 (≠ A322)
- binding butyramide: T40 (= T40), H61 (= H60), W87 (= W86), C148 (= C147)
- binding zinc ion: C38 (= C38), H61 (= H60), E62 (= E61), C92 (= C91), C95 (= C94), C98 (= C97), C106 (= C105), C148 (= C147)
6n7lC Crystal structure of an alcohol dehydrogenase from elizabethkingia anophelis nuhp1
32% identity, 99% coverage: 1:325/327 of query aligns to 8:340/344 of 6n7lC
- active site: C45 (= C38), T47 (= T40), H50 (= H43), H68 (= H60), C154 (= C147)
- binding nicotinamide-adenine-dinucleotide: C45 (= C38), H46 (≠ R39), T47 (= T40), H50 (= H43), C154 (= C147), T158 (≠ I151), G178 (= G169), G180 (= G171), G181 (≠ A172), L182 (≠ A173), D201 (≠ T192), V202 (≠ R193), K206 (≠ S197), T243 (≠ P236), A244 (≠ P237), V245 (≠ A238), S246 (≠ L239), A249 (≠ T242), N266 (≠ S256), G267 (≠ D257), L268 (≠ I258), I291 (≠ A276), V292 (≠ N277)
- binding zinc ion: C45 (= C38), H68 (= H60), C98 (= C91), C101 (= C94), C104 (= C97), C112 (= C105), C154 (= C147)
3meqA Crystal structure of alcohol dehydrogenase from brucella melitensis
33% identity, 99% coverage: 1:325/327 of query aligns to 3:338/341 of 3meqA
- active site: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), H63 (= H60), E64 (= E61), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), L112 (≠ R109), C150 (= C147), T154 (≠ I151), R335 (≠ A322)
- binding 1,4-dihydronicotinamide adenine dinucleotide: C40 (= C38), H41 (≠ R39), T42 (= T40), H45 (= H43), C150 (= C147), T154 (≠ I151), G176 (= G171), G177 (≠ A172), L178 (≠ A173), D197 (≠ T192), I198 (≠ R193), K202 (≠ G199), T241 (≠ G232), A242 (= A233), V243 (≠ L234), S244 (≠ V235), A247 (= A238), N264 (≠ A251), G265 (= G252), L266 (≠ I253), I289 (≠ A276), V290 (≠ N277)
- binding zinc ion: C40 (= C38), H63 (= H60), C94 (= C91), C97 (= C94), C100 (= C97), C108 (= C105), C150 (= C147)
6z42A The low resolution structure of a zinc-dependent alcohol dehydrogenase from halomonas elongata.
34% identity, 99% coverage: 1:325/327 of query aligns to 4:333/336 of 6z42A
- active site: C41 (= C38), T43 (= T40), H46 (= H43), H64 (= H60), C148 (= C147)
- binding zinc ion: C41 (= C38), H64 (= H60), E65 (= E61), C95 (= C91), C98 (= C94), C101 (= C97), C109 (= C105), C148 (= C147)
5yatA Crystal structure of mitochondrial alcohol dehydrogenase isozyme iii from komagataella phaffii gs115 (see paper)
39% identity, 48% coverage: 2:159/327 of query aligns to 7:165/347 of 5yatA
- active site: C43 (= C38), T45 (= T40), H48 (= H43), H66 (= H60), C153 (= C147)
- binding zinc ion: C43 (= C38), H66 (= H60), E67 (= E61), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), C153 (= C147)
2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
39% identity, 56% coverage: 1:182/327 of query aligns to 1:192/347 of 2eerB
- active site: C38 (= C38), H39 (≠ R39), S40 (≠ T40), H43 (= H43), H68 (= H60), E69 (= E61), E98 (≠ W89), C101 (= C94), C104 (= C97), C112 (= C105), R116 (= R109), C154 (= C147), T158 (≠ I151)
- binding nicotinamide-adenine-dinucleotide: C38 (= C38), H39 (≠ R39), S40 (≠ T40), H43 (= H43), C154 (= C147), T158 (≠ I151), G178 (= G169), G181 (≠ A172), G182 (≠ A173), L183 (vs. gap)
- binding zinc ion: E98 (≠ W89), C101 (= C94), C104 (= C97), C112 (= C105)
Sites not aligning to the query:
- active site: 342
- binding nicotinamide-adenine-dinucleotide: 203, 204, 205, 247, 248, 270, 271, 272, 273, 295, 296, 342
4eezB Crystal structure of lactococcus lactis alcohol dehydrogenase variant re1 (see paper)
28% identity, 99% coverage: 3:325/327 of query aligns to 4:336/342 of 4eezB
- active site: C39 (= C38), H40 (≠ R39), T41 (= T40), H44 (= H43), H60 (= H60), E61 (= E61), C91 (= C91), C94 (= C94), C97 (= C97), C105 (= C105), K109 (≠ R109), C147 (= C147), T151 (≠ I151), R333 (≠ A322)
- binding zinc ion: C39 (= C38), H60 (= H60), E61 (= E61), C91 (= C91), C94 (= C94), C97 (= C97), C105 (= C105), C147 (= C147)
5envA Yeast alcohol dehydrogenase with bound coenzyme (see paper)
34% identity, 64% coverage: 2:209/327 of query aligns to 7:216/347 of 5envA
- active site: C43 (= C38), H44 (≠ R39), T45 (= T40), H48 (= H43), H66 (= H60), E67 (= E61), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), D115 (≠ R109), C153 (= C147)
- binding trifluoroethanol: T45 (= T40), W54 (≠ L49), H66 (= H60), W92 (= W86), C153 (= C147)
- binding nicotinamide-adenine-dinucleotide: H44 (≠ R39), T45 (= T40), H48 (= H43), T157 (≠ I151), G177 (= G169), G180 (= G171), G181 (≠ A172), L182 (≠ A173), D201 (= D195), K206 (vs. gap)
- binding zinc ion: C43 (= C38), H66 (= H60), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), C153 (= C147)
Sites not aligning to the query:
4w6zA Yeast alcohol dehydrogenase i, saccharomyces cerevisiae fermentative enzyme (see paper)
34% identity, 64% coverage: 2:209/327 of query aligns to 7:216/347 of 4w6zA
- active site: C43 (= C38), H44 (≠ R39), T45 (= T40), H48 (= H43), H66 (= H60), E67 (= E61), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), D115 (≠ R109), C153 (= C147)
- binding nicotinamide-8-iodo-adenine-dinucleotide: C43 (= C38), H44 (≠ R39), T45 (= T40), H48 (= H43), W54 (≠ L49), C153 (= C147), T157 (≠ I151), G177 (= G169), G180 (= G171), G181 (≠ A172), L182 (≠ A173), I200 (≠ F191), D201 (= D195), K206 (vs. gap)
- binding trifluoroethanol: T45 (= T40), W54 (≠ L49), H66 (= H60), W92 (= W86), C153 (= C147)
- binding zinc ion: C43 (= C38), H66 (= H60), C97 (= C91), C100 (= C94), C103 (= C97), C111 (= C105), C153 (= C147)
Sites not aligning to the query:
- active site: 340
- binding nicotinamide-8-iodo-adenine-dinucleotide: 221, 246, 248, 251, 268, 269, 270, 293, 294, 295, 340
P00330 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; ADHI; NADH-dependent methylglyoxal reductase; YADH-1; EC 1.1.1.1; EC 1.1.1.54; EC 1.1.1.78 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
34% identity, 64% coverage: 2:209/327 of query aligns to 8:217/348 of P00330
- C44 (= C38) binding
- H45 (≠ R39) binding ; mutation to R: Decreases dissociation constants by 4-fold for NAD(+) and 2-fold for NADH, while turnover numbers were decreased by 4-fold for ethanol oxidation and 6-fold for acetaldehyde reduction.
- T46 (= T40) binding ; mutation to S: Has the same pattern of activity as the wild-type enzyme for linear primary alcohols.
- H49 (= H43) binding
- W55 (≠ L49) mutation to M: Has lowered reactivity with primary and secondary alcohols.
- H67 (= H60) binding
- E68 (= E61) binding in the open conformation
- W93 (= W86) mutation to A: Has an inverted specificity pattern for primary alcohols, being 3- and 10-fold more active on hexanol and 350- and 540-fold less active on ethanol. Also acquires weak activity on branched chain alcohols and cyclohexanol.
- C98 (= C91) binding
- C101 (= C94) binding
- C104 (= C97) binding
- C112 (= C105) binding
- C154 (= C147) binding
- G181 (= G171) binding
- G182 (≠ A172) binding
- L183 (≠ A173) binding
- D202 (= D195) binding
- K207 (vs. gap) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylserine
- 222 binding
- 236 natural variant: T -> I
- 269 binding
- 271 binding ; M→L: Produces a 7 to 10-fold increase in reactivity with butanol, pentanol, and hexanol.
- 294 binding
- 296 binding
- 341 binding
Query Sequence
>Pf1N1B4_4176 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4176
MRAMVLHAPGQPLQREERAIPIPDAHQLLIKVLACGVCRTDLHLVDGELPQAVLPRVPGH
EIVGEVTAVGANVAPDWVGKRVGVPWLGWTCGECTFCRSGRENLCDRARFTGCHLDGGYA
EYTVADAHFCFPIPDALSAAEAAPLLCAGLIGFRALQMAKGARHLGLYGFGAAAHLAIQV
ARGRGQQVYAFTRPDDSEGQAYARTLGAEWAGPSDQPPPHPLDASLIFAPVGALVPPALE
ATVKGGCVICAGIHMSDIPSFPYRLLWGERSIRSVANLTREDGTAFFAELRHTPVHSDVT
CFALDDANQALASLRAGQVKGAIVLIP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory