SitesBLAST – Find functional sites

 

SitesBLAST

Comparing Pf1N1B4_4706 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4706 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q4U331 Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase; Pyr2C/Pip2C reductase; N-methyl-L-amino acid dehydrogenase; EC 1.5.1.21; EC 1.4.1.17 from Pseudomonas syringae pv. tomato (see paper)
62% identity, 98% coverage: 1:63/64 of query aligns to 280:342/343 of Q4U331

query
sites
Q4U331
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P
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E
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G

Sites not aligning to the query:

2cwfB Crystal structure of delta1-piperideine-2-carboxylate reductase from pseudomonas syringae complexed with NADPH (see paper)
62% identity, 98% coverage: 1:63/64 of query aligns to 274:336/337 of 2cwfB

query
sites
2cwfB
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P
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D
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F
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A
E
 
Q
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R
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S
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E
E
 
E
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L
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V
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R
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Q
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L
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H
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G
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G

Sites not aligning to the query:

2cwhA Crystal structure of delta1-piperideine-2-carboxylate reductase from pseudomonas syringae complexed with NADPH and pyrrole-2-carboxylate (see paper)
61% identity, 97% coverage: 1:62/64 of query aligns to 271:332/332 of 2cwhA

query
sites
2cwhA
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V
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P
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D
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E
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E
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L
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H
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V
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L
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P
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D
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Y
R
 
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E
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A
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A
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G
 
G
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L
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R
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L
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Q
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E
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L
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A

Sites not aligning to the query:

Query Sequence

>Pf1N1B4_4706 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4706
VIDPSKSAGQNFAERSQELVRQMHGVGLKRLPGDRRHRQRAKAGVEGIVLDAQTLANLRE
LAGL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory