SitesBLAST
Comparing Pf1N1B4_5586 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5586 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3gobA Crystal structure of dicamba monooxygenase with non-heme cobalt and dcsa (see paper)
36% identity, 98% coverage: 2:342/348 of query aligns to 4:340/342 of 3gobA
- binding cobalt (ii) ion: N154 (= N150), H160 (= H156), H165 (= H161), D294 (= D298)
- binding fe2/s2 (inorganic) cluster: C49 (= C47), H51 (= H49), R52 (= R50), A54 (= A52), C68 (= C66), Y70 (= Y68), H71 (= H69), L73 (= L71)
- binding 3,6-dichloro-2-hydroxybenzoic acid: N230 (≠ I230), I232 (≠ V232), H251 (≠ D256), Q286 (= Q291)
3gb4A Crystal structure of dicamba monooxygenase with non-heme cobalt and dicamba (see paper)
36% identity, 98% coverage: 2:342/348 of query aligns to 3:339/341 of 3gb4A
- binding cobalt (ii) ion: H159 (= H156), H164 (= H161), D293 (= D298)
- binding 3,6-dichloro-2-methoxybenzoic acid: L157 (= L154), N229 (≠ I230), I231 (≠ V232), H250 (≠ D256), W284 (≠ G290)
- binding fe2/s2 (inorganic) cluster: C48 (= C47), H50 (= H49), R51 (= R50), A53 (= A52), C67 (= C66), Y69 (= Y68), H70 (= H69), L72 (= L71)
3gkeA Crystal structure of dicamba monooxygenase (see paper)
36% identity, 98% coverage: 2:342/348 of query aligns to 3:339/340 of 3gkeA
Q5S3I3 Dicamba O-demethylase, oxygenase component; Dicamba monooxygenase; DMO; Three-component Rieske non-heme iron oxygenase system; EC 1.14.15.- from Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia) (see 3 papers)
36% identity, 98% coverage: 2:342/348 of query aligns to 3:339/339 of Q5S3I3
- C48 (= C47) binding
- H50 (= H49) binding
- C67 (= C66) binding
- H70 (= H69) binding
- N153 (= N150) Plays a role in the stabilization of the metal coordination; mutation to A: Strong reduction of O-demethylase activity.
- D156 (= D153) mutation to N: Strong reduction of O-demethylase activity.
- H159 (= H156) binding ; mutation to N: Loss of O-demethylase activity.
- H164 (= H161) binding ; mutation to N: Loss of O-demethylase activity.
- N229 (≠ I230) binding
- H250 (≠ D256) binding
- W284 (≠ G290) binding
- D293 (= D298) binding ; mutation to N: Loss of O-demethylase activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3gkeB Crystal structure of dicamba monooxygenase (see paper)
35% identity, 98% coverage: 2:342/348 of query aligns to 3:333/334 of 3gkeB
- binding fe (iii) ion: N153 (= N150), H159 (= H156), H164 (= H161), D287 (= D298)
- binding fe2/s2 (inorganic) cluster: C48 (= C47), H50 (= H49), R51 (= R50), A53 (= A52), C67 (= C66), Y69 (= Y68), H70 (= H69), L72 (= L71)
- binding oxygen molecule: H159 (= H156), H164 (= H161), D287 (= D298)
6vshC Crystal structure of apo dicamba monooxygenase (see paper)
35% identity, 98% coverage: 2:342/348 of query aligns to 3:318/320 of 6vshC
7qwtA Rieske non-heme iron monooxygenase for guaiacol o-demethylation
29% identity, 97% coverage: 4:341/348 of query aligns to 8:343/352 of 7qwtA
7szeB Structure of the rieske non-heme iron oxygenase gxta with saxitoxin bound (see paper)
25% identity, 95% coverage: 5:336/348 of query aligns to 10:322/329 of 7szeB
- binding [(3aS,4R,10aS)-2,6-diamino-10,10-dihydroxy-3a,4,9,10-tetrahydro-3H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate: S159 (≠ L151), F165 (≠ E157), L173 (≠ I165), N216 (≠ D215), Q226 (≠ E231), C228 (≠ G233), D239 (≠ S252), M241 (≠ V254), Y255 (≠ W270), D272 (≠ G290), Y273 (≠ Q291), V276 (≠ I294)
- binding fe (iii) ion: N158 (= N150), H164 (= H156), H169 (= H161), D280 (= D298)
- binding fe2/s2 (inorganic) cluster: C55 (= C47), H57 (= H49), R58 (= R50), V60 (≠ A52), C74 (= C66), Y76 (= Y68), H77 (= H69), W79 (≠ L71)
6wndA Structure of the rieske non-heme iron oxygenase gxta with dideoxysaxitoxin bound (see paper)
31% identity, 54% coverage: 5:192/348 of query aligns to 9:203/311 of 6wndA
- binding fe (iii) ion: N157 (= N150), H163 (= H156), H168 (= H161)
- binding fe2/s2 (inorganic) cluster: C54 (= C47), H56 (= H49), R57 (= R50), V59 (≠ A52), C73 (= C66), Y75 (= Y68), H76 (= H69), W78 (≠ L71)
- binding [(2Z,3aS,4R,6Z,10aR)-2,6-diiminooctahydro-1H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate: S158 (≠ L151), F164 (≠ E157), I171 (≠ S164), L172 (≠ I165), N203 (= N192)
Sites not aligning to the query:
- binding fe (iii) ion: 267
- binding [(2Z,3aS,4R,6Z,10aR)-2,6-diiminooctahydro-1H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate: 213, 215, 226, 242, 259, 260, 263
7szgA Structure of the rieske non-heme iron oxygenase gxta pressurized with xenon (see paper)
30% identity, 53% coverage: 5:189/348 of query aligns to 8:196/315 of 7szgA
Sites not aligning to the query:
7szfA Structure of the rieske non-heme iron oxygenase gxta with beta- saxitoxinol bound (see paper)
30% identity, 53% coverage: 5:189/348 of query aligns to 9:197/318 of 7szfA
- binding beta-Saxitoxinol: S158 (≠ L151), F164 (≠ E157)
- binding fe (iii) ion: N157 (= N150), H163 (= H156), H168 (= H161)
- binding fe2/s2 (inorganic) cluster: C54 (= C47), H56 (= H49), R57 (= R50), V59 (≠ A52), C73 (= C66), Y75 (= Y68), H76 (= H69), W78 (≠ L71)
Sites not aligning to the query:
6wn3B Structure of the rieske non-heme iron oxygenase sxtt (see paper)
31% identity, 47% coverage: 5:168/348 of query aligns to 10:176/328 of 6wn3B
Sites not aligning to the query:
7szhA Structure of the rieske non-heme iron oxygenase sxtt with beta- saxitoxinol bound (see paper)
31% identity, 47% coverage: 5:168/348 of query aligns to 8:174/323 of 7szhA
- binding beta-Saxitoxinol: S157 (≠ L151), F163 (≠ E157), L171 (≠ I165)
- binding fe (iii) ion: N156 (= N150), H162 (= H156), H167 (= H161)
- binding fe2/s2 (inorganic) cluster: C53 (= C47), H55 (= H49), R56 (= R50), V58 (≠ A52), C72 (= C66), Y74 (= Y68), H75 (= H69), W77 (≠ L71)
Sites not aligning to the query:
- binding beta-Saxitoxinol: 202, 212, 222, 224, 225, 226, 233, 234, 235, 251, 269, 272, 273
- binding fe (iii) ion: 276
6wnbA Structure of the rieske non-heme iron oxygenase sxtt with dideoxysaxitoxin bound (see paper)
31% identity, 47% coverage: 5:168/348 of query aligns to 9:175/320 of 6wnbA
- binding fe (iii) ion: N157 (= N150), H163 (= H156), H168 (= H161)
- binding fe2/s2 (inorganic) cluster: C54 (= C47), H56 (= H49), R57 (= R50), V59 (≠ A52), C73 (= C66), Y75 (= Y68), H76 (= H69), W78 (≠ L71)
- binding [(2Z,3aS,4R,6Z,10aR)-2,6-diiminooctahydro-1H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate: S158 (≠ L151), F164 (≠ E157), L172 (≠ I165)
Sites not aligning to the query:
- binding fe (iii) ion: 273
- binding [(2Z,3aS,4R,6Z,10aR)-2,6-diiminooctahydro-1H,8H-pyrrolo[1,2-c]purin-4-yl]methyl carbamate: 203, 209, 219, 221, 222, 223, 230, 231, 232, 248, 266, 269, 270
Q9MBA1 Chlorophyllide a oxygenase, chloroplastic; Chlorophyll a oxygenase; Chlorophyll b synthase; AtCAO; EC 1.14.13.122 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 94% coverage: 4:330/348 of query aligns to 218:519/536 of Q9MBA1
Sites not aligning to the query:
- 97:106 mutation Missing: Increased stability of the protein.
7fhrA Crystal structure of a rieske oxygenase from cupriavidus metallidurans (see paper)
30% identity, 41% coverage: 19:162/348 of query aligns to 42:188/437 of 7fhrA
- binding fe (ii) ion: H182 (= H156), H187 (= H161)
- binding fe2/s2 (inorganic) cluster: C70 (= C47), H72 (= H49), R73 (= R50), A75 (= A52), C89 (= C66), Y91 (= Y68), H92 (= H69), W94 (≠ L71)
- binding glutamic acid: E177 (≠ L151), H182 (= H156), H187 (= H161)
- binding glycine: R81 (≠ Y58), R88 (≠ V65)
Sites not aligning to the query: