SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_5613 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5613 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 11 hits to proteins with known functional sites (download)

P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
60% identity, 99% coverage: 2:317/318 of query aligns to 21:329/333 of P13804

query
sites
P13804

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G116 (= G104) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
T171 (= T159) to I: decreased protein stability; dbSNP:rs1801591
R223 (= R211) binding
S248 (= S236) binding
R249 (= R237) mutation to A: Loss of electron transfer activity.
VGQT 263:266 (= VGQT 251:254) binding
T266 (= T254) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
SGAIQH 281:286 (= SGAIQH 269:274) binding
N300 (= N288) binding
DL 318:319 (= DL 306:307) binding

Sites not aligning to the query:

20:204 Domain I
205:333 Domain II

2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
60% identity, 99% coverage: 2:317/318 of query aligns to 3:311/315 of 2a1uA

query
sites
2a1uA

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binding flavin-adenine dinucleotide: G204 (= G210), R205 (= R211), S230 (= S236), R231 (= R237), A232 (= A238), Q244 (= Q250), V245 (= V251), G246 (= G252), T248 (= T254), G261 (= G267), I262 (= I268), S263 (= S269), A265 (= A271), Q267 (= Q273), H268 (= H274), N282 (= N288), K283 (= K289), D300 (= D306), L301 (= L307)

1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
63% identity, 88% coverage: 35:314/318 of query aligns to 29:303/307 of 1efpA

query
sites
1efpA

G

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binding flavin-adenine dinucleotide: G199 (= G210), R200 (= R211), G201 (= G212), S225 (= S236), R226 (= R237), A227 (= A238), Q239 (= Q250), V240 (= V251), G241 (= G252), T243 (= T254), G256 (= G267), I257 (= I268), S258 (= S269), A260 (= A271), Q262 (= Q273), H263 (= H274), N277 (= N288), K278 (= K289), D295 (= D306), L296 (= L307)

5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
38% identity, 77% coverage: 71:314/318 of query aligns to 45:287/292 of 5ow0A

query
sites
5ow0A

A

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G

A

A

S
|
S

R
|
R

A

G

A

A

V

V

D

D

A

E

G

G

F

W

V

M

P

P

N

P

D

G

M

A

Q
|
Q

V
x
I

G
|
G

Q

Q

T
|
T

G

G

K

K

I

I

V

V

A

A

P

P

Q

T

L

V

Y

Y

I

F

A

A

V

V

G
|
G

I
x
V

S
|
S

G

G

A
|
A

I

S

Q
|
Q

H
|
H

L

L

A

A

G

G

M

I

K

S

D

N

S

A

K

K

V

C

I

V

V

I

A

A

I

I

N
|
N

K
|
K

D

D

E

N

E

E

A

A

P

N

I

I

F

F

Q

K

V

R

A

A

D

R

Y

F

G

G

L

I

V

V

A

G

D
|
D

L
x
Y

F

K

E

K

A

A

I

V

P

P

E

A

M

L

binding flavin-adenine dinucleotide: G183 (= G210), R184 (= R211), G185 (= G212), S209 (= S236), R210 (= R237), Q223 (= Q250), I224 (≠ V251), G225 (= G252), T227 (= T254), G240 (= G267), V241 (≠ I268), S242 (= S269), A244 (= A271), Q246 (= Q273), H247 (= H274), N261 (= N288), K262 (= K289), D279 (= D306), Y280 (≠ L307)

4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 80% coverage: 62:316/318 of query aligns to 70:330/338 of 4kpuA

query
sites
4kpuA

V

V

L

Y

V

V

A

C

D

Q

N

D

A

P

A

A

Y

F

A

K

Y

Y

Q

Y

L

S

P

T

E

D

N

E

V

Y

A

T

P

N

L

A

V

F

A

C

G

E

L

M

V

I

I

D

E

E

Q

Y

R

Q

T

P

I

-

Y

-

S

S

H

S

V

V

L

F

A

I

S

G

A

A

T

T

S

N

N

D

G

G

K

R

N

D

I

L

L

G

P

P

R

R

V

I

A

A

Q

R

L

V

D

N

A

T

D

G

Q
x
L

I

C

S

A

E

D

I

C

I

T

S

I

V

L

E

D

S

A

A

E

D

E

-

D

-

G

-

L

-

I

T

E

F

W

K

T

R
|
R

P

P

I

A

Y

A

A

G

G

G

N

N

A

I

I

M

A

A

T

T

V
x
I

Q

L

S

C

N

K

A

E

A

H

V

R

K

P

V

Q

I

M

-

G

T

T

V

V

R

R

A

P

T

K

G

T

F

F

-

K

-

A

-

M

D

E

P

P

V

D

A

A

A

S

E

R

G

T

G

G

S

E

A

V

A

I

V

N

E

Y

A

T

V

L

A

K

A

N

A

H

H

V

D

D

A

D

G

R

I

V

S

T

T

C

F

I

V

R

G

R

E

E

L

V

A

V

K

S

S

E

D

G

R

E

P

M

E

A

L

I

T

D

A

D

A

A

K

P

I

F

V

V

V

C

S

S

G

G

G
|
G

R
|
R

G
|
G

M

M

Q

K

N

A

G

K

D

E

N

N

F

F

K

S

H

L

L

L

Y

Y

A

D

L

L

A

A

D

H

K

A

L

L

G

G

A

G

A

A

V

V

G

G

A

G

S
|
S

R
|
R

A
|
A

A

A

V

V

D

D

A

E

G

G

F

F

V

I

P

E

N

H

D

P

M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

T

V

V

A

T

P

P

Q

K

L

I

Y

Y

I

F

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

V

Q
|
Q

H
|
H

L

K

A

A

G

G

M

M

K

S

D

K

S

S

K

D

V

T

I

I

V

V

A

C

I

I

N
|
N

K
|
K

D

D

E

P

E

D

A

A

P

P

I

M

F

F

Q

E

V

I

A

S

D

K

Y

Y

G

G

L

I

V

V

A

G

D
|
D

L
x
A

F

L

E

K

A

I

I

L

P

P

E

L

M

L

A

T

A

A

binding flavin-adenine dinucleotide: L125 (≠ Q119), R144 (= R134), I155 (≠ V145), G224 (= G210), R225 (= R211), G226 (= G212), S250 (= S236), R251 (= R237), A252 (= A238), Q264 (= Q250), V265 (= V251), G266 (= G252), Q267 (= Q253), S268 (≠ T254), G281 (= G267), I282 (= I268), S283 (= S269), S285 (≠ A271), Q287 (= Q273), H288 (= H274), N302 (= N288), K303 (= K289), D320 (= D306), A321 (≠ L307)

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
36% identity, 95% coverage: 12:312/318 of query aligns to 73:382/393 of 6fahE

query
sites
6fahE

D

E

A

Q

Q

R

D

E

N

G

K

K

V

I

L

M

A

-

P

P

A

V

T

V

L

F

N

E

T

L

V

L

A

G

A

E

A

G

A

K

K

K

I

L

G

A

G

N

D

E

I

I

H

G

V

T

L

E

V

L

A

C

G

A

A

I

F

L

V

C

G

G

S

S

-

N

V

V

A

A

E

E

A

L

A

T

A

D

K

E

I

L

-

F

-

A

A

Y

G

G

V

A

A

D

K

K

V

V

L

Y

V

L

A

A

D

D

N

-

A

-

Y

-

A

A

Y

P

Q

E

L

L

P

E

E

K

N

Y

V

T

A

T

P

D

L

G

V

Y

A

S

G

K

L

I

V

I

I

N

E

E

Q

A

R

I

T

G

I

L

Y

Y

S

K

H

P

-

E

-

I

V

V

L

L

A

Y

S

G

A

A

T

T

S

H

N

I

G

G

K

R

N

D

I

L

L

A

P

P

R

C

V

L

A

A

A

V

Q

K

L

V

D

N

A

T

D

G

Q
x
L

I

T

S

A

E

D

I

C

I

T

S

K

V

L

E

E

-

I

S

D

A

P

D

D

T

D

F

K

K

K

-

I

-

R

-

Q

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

L

I

M

A

A

T

T

-

I

V

V

Q

C

S

P

N

G

A

S

A

R

V

P

K

Q

V

M

I

S

T

T

V

V

R

R

A

P

T

G

G

V

F

M

D

D

P

K

V

A

A

A

A

Y

E

D

G

P

G

S

S

Q

A

K

A

G

-

E

-

V

V

I

E

K

A

L

V

D

A

A

A

T

A

F

H

N

D

E

A

G

G

D

I

I

S

R

T

T

F

K

V

V

G

L

E

E

E

I

L

V

-

K

A

T

K

T

S

T

D

D

R

N

P

I

E

S

L

I

T

S

A

D

A

A

K

D

I

F

V

I

V

V

S

S

G

G

R
x
M

G
|
G

M

L

Q

G

N

K

G

P

D

E

N

G

F

F

K

E

H

L

L

L

Y

K

A

Q

L

L

A

A

D

D

K

K

L

L

G

G

A

G

A

T

V

V

G

A

A

T

S

S

R
|
R

A
|
A

A

C

V

V

D

D

A

A

G

G

F

W

V

A

P

D

N

H

D

A

M

Q

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
|
T

G

G

K

T

I

T

V

V

A

K

P

P

Q

Q

L

I

Y

Y

I

F

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

Q

D

D

S

S

K

D

V

I

I

I

V

I

A

A

I

I

N
|
N

K
|
K

D

N

E

E

E

N

A

A

P

P

I

I

F

F

Q

E

V

V

A

A

D

D

Y

Y

G

G

L

I

V

V

A

G

D

D

L
|
L

F

Y

E

K

A

V

I

I

P

P

binding flavin-adenine dinucleotide: L180 (≠ Q119), R200 (= R134), M281 (≠ R211), G282 (= G212), R307 (= R237), A308 (= A238), Q320 (= Q250), V321 (= V251), G322 (= G252), Q323 (= Q253), T324 (= T254), G337 (= G267), I338 (= I268), S339 (= S269), Q343 (= Q273), H344 (= H274), N358 (= N288), K359 (= K289), L377 (= L307)

Sites not aligning to the query:

binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49

5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
34% identity, 95% coverage: 14:314/318 of query aligns to 10:320/331 of 5ol2A

query
sites
5ol2A

Q

Q

D

R

N

E

K

N

V

V

L

I

A

Q

P

T

A

V

T

S

L

L

N

E

T

L

V

L

A

G

A

K

A

A

A

T

K

E

I

I

G

A

G

K

D

D

I

Y

H

D

V

T

L

K

V

V

A

S

G

A

A

L

F

L

V

L

G

G

S

S

V

K

A

V

E

E

A

G

A

L

A

I

K

D

I

T

A

L

-

A

-

H

-

Y

G

G

V

A

A

D

K

E

V

V

L

I

V

V

A

V

D

D

N

D

A

E

A

A

Y

L

A

A

-

V

-

Y

-

T

-
x
E

-

P

-

Y

-

T

-

K

-

A

Y

Y

Q

E

L

A

P

I

E

K

N

A

V

A

A

D

P

P

L

I

V

V

A

-

G

-

L

-

V

-

I

-

E

-

Q

-

R

-

T

-

I

-

Y

-

S

-

H

-

V

V

L

L

A

F

S

G

A

A

T

T

S

S

N

I

G

G

K

R

N

D

I

L

L

A

P

P

R

R

V

V

A

S

A

A

Q

R

L

I

D

H

A

T

D

G

Q

L

I
x
T

S

A

E

D

I

C

I

T

S

G

V

L

E

A

S

V

A

A

D

E

T

D

F

T

K

K

-

L

-

M

-

T

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A

A

T

T

-
x
I

V

V

Q

C

S

K

N

D

A

F

A

R

V

P

K

Q

V

M

I

S

T

T

V

V

R

R

A

P

T

G

G

V

F

M

D

K

P

K

V

N

A

E

A

P

E

D

G

E

G

T

S

K

A

E

A

A

V

V

-

I

-

N

E

R

A

F

V

K

A

V

A

E

A

F

H

N

D

D

A

A

G
x
D

I

K

S

L

T

V

F

Q

V

V

G

V

E

Q

E

V

L

I

A

K

K

E

S

A

D

K

R

K

P

Q

-

V

E

K

L

I

T

E

A

D

A

A

K

K

I

I

V

L

V

V

S

S

G

A

G
|
G

R
|
R

G
|
G

M

M

Q

G

N

G

G

K

D

E

N

N

F

L

K

D

H

I

L

L

Y

Y

A

E

L

L

A

A

D

E

K

I

L

I

G

G

A

G

A

E

V

V

G

S

A

G

S
|
S

R
|
R

A
|
A

A

T

V

I

D

D

A

A

G

G

F

W

V

L

P

D

N

K

D

A

M

R

Q
|
Q

V
|
V

G
|
G

Q

Q

T
|
T

G

G

K

K

I

T

V

V

A

R

P

P

Q

D

L

L

Y

Y

I

I

A

A

V

C

G
|
G

I
|
I

S
|
S

G

G

A
|
A

I

I

Q
|
Q

H
|
H

L

I

A

A

G

G

M

M

K

E

D

D

S

A

K

E

V

F

I

I

V

V

A

A

I

I

N
|
N

K
|
K

D

N

E

P

E

E

A

A

P

P

I

I

F

F

Q

K

V

Y

A

A

D

D

Y

V

G

G

L

I

V

V

A

G

D
|
D

L
x
V

F

H

E

K

A

V

I

L

P

P

E

E

M

L

binding calcium ion: E75 (vs. gap), D188 (≠ G183)
binding flavin-adenine dinucleotide: T117 (≠ I120), R136 (= R134), I147 (vs. gap), G216 (= G210), R217 (= R211), G218 (= G212), S242 (= S236), R243 (= R237), A244 (= A238), Q256 (= Q250), V257 (= V251), G258 (= G252), T260 (= T254), G273 (= G267), I274 (= I268), S275 (= S269), A277 (= A271), Q279 (= Q273), H280 (= H274), N294 (= N288), K295 (= K289), D312 (= D306), V313 (≠ L307)

7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 94% coverage: 19:317/318 of query aligns to 24:330/337 of 7qh2A

query
sites
7qh2A

L

I

A

H

P

P

A

V

T

T

L

F

N

E

T

L

V

I

A

G

A

K

A

A

-

R

-

E

-

L

-

A

A

A

K

V

I

I

G

G

G

H

D

P

I

V

H

Y

V

A

L

L

V

L

A

M

G

G

A

T

F

N

V

I

G

T

S

E

V

K

A

A

E

D

A

E

A

L

K

K

I

Y

A

-

G

G

V

V

A

D

K

K

V

V

L

F

V

V

A

Y

D

D

N

K

A

P

A

E

Y

L

A

K

Y

H

Q

F

L

V

P

I

E

E

N

P

V

Y

A

A

P

N

L

V

V

L

A

E

G

D

L

F

V

I

I

-

E

-

Q

E

R

K

T

V

I

K

Y

P

S

S

H

S

V

I

L

L

A

V

S

G

A

A

T

T

S

N

N

V

G

G

K

R

N

S

I

L

L

A

P

P

R

R

V

V

A

A

Q

R

-

Y

-

R

-

T

-

G

L
|
L

D
x
T

A

A

D

D

Q

-

I

-

S

C

E

T

I

I

I

L

S

E

V

M

-

K

E

E

S

N

A

T

D

D

T

L

F

V

K

Q

-

I

R
|
R

P

P

I

A

Y

F

A

G

G

G

N

N

A

I

I

M

A

A

T

Q

-
x
I

V

V

Q

T

S

E

N

N

A

T

A

R

V

P

K

Q

V

F

I

C

T

T

V

V

R

R

A

Y

T

K

G

V

F

F

D

T

-

A

P

P

V

E

A

R

A

V

E

N

G

E

G

P

S

W

A

G

A

D

V

V

E

E

A

M

V

M

A

D

A

I

A

E

H

K

D

A

A

K

G

L

I

V

S

S

T

A

F

I

-

E

V

V

G

M

E

E

E

V

L

I

A

K

K

K

S

E

D

K

R

G

P

I

E

D

L

L

T

S

A

E

A

A

K

E

I

T

V

I

V

V

S

A

G

V

G

G

R
|
R

G
|
G

M

V

Q

K

N

C

G

E

D

K

N

D

F

L

K

D

H

M

L

I

Y

H

A

E

L

F

A

A

D

E

K

K

L

I

G

G

A

A

A

T

V

V

G

A

A

C

S
x
T

R
|
R

A

P

A

G

V

I

D

E

A

A

G

G

F

W

V

F

P

D

N

A

D

R

M

L

Q
|
Q

V
x
I

G
|
G

Q
x
L

T
x
S

G

G

K

R

I

T

V

V

A

K

P

P

Q

K

L

L

Y

I

I

I

A

A

V

L

G
|
G

I
|
I

S
|
S

G

G

A

A

I

V

Q
|
Q

H

F

L

A

A

A

G

G

M

M

K

Q

D

N

S

S

K

E

V

Y

I

I

V

I

A

A

I

I

N
|
N

K
x
S

D
|
D

E

P

E

K

A

A

P

P

I

I

F

F

Q

N

V

I

A

A

D

H

Y

C

G

G

L

M

V

V

A

G

D
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D

L
|
L

F

Y

E

E

A

I

I

L

P

P

E

E

M

L

A

L

A

T

L

M

binding flavin-adenine dinucleotide: L125 (= L115), T126 (≠ D116), R144 (= R134), I155 (vs. gap), R224 (= R211), G225 (= G212), T249 (≠ S236), R250 (= R237), Q263 (= Q250), I264 (≠ V251), G265 (= G252), L266 (≠ Q253), S267 (≠ T254), G280 (= G267), I281 (= I268), S282 (= S269), Q286 (= Q273), N301 (= N288), S302 (≠ K289), D303 (= D290), D319 (= D306), L320 (= L307)

7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
37% identity, 87% coverage: 41:316/318 of query aligns to 41:325/336 of 7koeB

query
sites
7koeB

V

V

A

W

G

G

A

V

F

L

V

L

G

G

S

E

V

G

A

L

E

E

A

S

A

V

A

A

K

K

I

E

A

A

-

I

-

Q

-

R

G

G

V

A

A

D

K

K

V

V

L

L

V

Y

A

V

D

K

N

N

A

R

A

E

Y

F

A

N

Y

T

Q

Y

L

V

P

N

E

Y

N

L

V

Y

A

K

P

K

L

A

V

L

A

V

G

D

L

M

V

V

I

R

E

K

Q

Y

R

R

T

P

I

-

Y

-

S

E

H

I

V

F

L

L

A

I

S

G

A

A

T

T

S

L

N

E

G

G

K

R

N

E

I

L

L

A

P

G

R

M

V

V

A

A

A

T

Q

E

L

L

D

E

-

T

-

G

-

L

-
x
T

A

A

D

D

Q

C

I

T

S

G

-

L

E

D

I

I

S

P

V

D

E

K

S

K

A

L

D

L

T

A

F

M

K

T

R
|
R

P

P

I
x
T

Y

F

A

G

G

G

N

N

A

L

I

M

A

A

T

T

V

I

Q

M

S

C

-

P

N

D

A

H

A

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I

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M

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K

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E

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L

A

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D

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P

G

E

S

R

A

T

A

G

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-

E

E

A

I

V

I

A

E

H

Y

D

D

A

L

G

G

I

-

S

-

T

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-

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-

L

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E

E

E

T

L

I

A

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Q

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E

N

L

L

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E

A

Y

A

A

K

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I

V

V

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S

A

G

G

G
|
G

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x
K

G
|
G

M

V

Q

G

N

G

G

P

D

E

N

G

F

F

K

K

H

K

L

L

Y

K

A

E

L

L

A

A

D

D

K

L

L

L

G

G

A

G

A

E

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V

G

G

A

A

S
|
S

R
|
R

A
|
A

A

A

V

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D

K

A

A

G

G

F

W

V

I

P

S

N

P

D

E

M

H

Q
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Q

V
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V

G
|
G

Q
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Q

T
|
T

G

G

K

K

I

T

V

V

A

R

P

P

Q

V

L

L

Y

Y

I

F

A

A

V

C

G
|
G

I

I

S
|
S

G

G

A

A

I

I

Q
|
Q

H
|
H

L

V

A

V

G

G

M

I

K

K

D

E

S

S

K

E

V

I

I

I

V

V

A

A

I

I

N
|
N

K
x
I

D

D

E

E

E

K

A

A

P

P

I

I

F

F

Q

D

V

I

A

A

D

D

Y

I

G

G

L

I

V

V

A

G

D

D

L
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L

F

H

E

K

A

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I

V

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P

E

A

M

L

A

T

A

A

binding flavin-adenine dinucleotide: T121 (vs. gap), R140 (= R134), T142 (≠ I136), G219 (= G210), K220 (≠ R211), G221 (= G212), S245 (= S236), R246 (= R237), A247 (= A238), Q259 (= Q250), V260 (= V251), G261 (= G252), Q262 (= Q253), T263 (= T254), G276 (= G267), S278 (= S269), Q282 (= Q273), H283 (= H274), N297 (= N288), I298 (≠ K289), L316 (= L307)

P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
38% identity, 52% coverage: 153:316/318 of query aligns to 153:317/321 of P53571

query
sites
P53571

V

V

I

L

T

T

V

I

R

R

A

P

T

S

G

V

F

F

D

K

P

P

V

L

A

E

A

G

E

A

G

G

G

S

S

P

-

V

A

S

A

N

V

V

E

D

A

A

V

P

A

S

A

V

A

Q

H

S

D

R

A

S

G

Q

I

N

S

K

T

D

F

Y

V

V

G

-

E

-

E

E

L

V

A

G

K

G

S

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

V

K

D

I

F

V

I

V

M

S

S

G

I

G

G

R
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R

G

G

M

I

Q

G

N

E

G

E

D

T

N

N

F

V

K

E

H

Q

L

F

Y

R

A

E

L

L

A

A

D

D

K

E

L

A

G

G

A

A

A

T

V

L

G

C

A

C

S
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S

R
|
R

A

P

A

I

V

A

D

D

A

A

G

G

F

W

V

L

P

P

N

K

D

S

M

R

Q
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Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

V

M

G
|
G

I
|
I

S
|
S

G
|
G

A
x
S

I
|
I

Q
|
Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

V

K

P

V

T

I

I

V

I

A

A

I

V

N
|
N

K

T

D

D

E

P

E

G

A

A

P

S

I

I

F

F

Q

T

V

I

A

A

D

K

Y

Y

G

G

L

I

V

V

A

A

D
|
D

L
x
I

F

F

E

D

A

I

I

E

P

E

E

E

M

L

A

K

A

A

R211 (= R211) binding
SR 236:237 (= SR 236:237) binding
QVGQS 250:254 (≠ QVGQT 250:254) binding
268:275 (vs. 267:274, 88% identical) binding
N289 (= N288) binding
DI 307:308 (≠ DL 306:307) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
38% identity, 52% coverage: 153:316/318 of query aligns to 152:316/319 of 3clrD

query
sites
3clrD

V

V

I

L

T

T

V

I

R

R

A

P

T

S

G

V

F

F

D

K

P

P

V

L

A

E

A

G

E

A

G

G

G

S

S

P

-

V

A

S

A

N

V

V

E

D

A

A

V

P

A

S

A

V

A

Q

H

S

D

R

A

S

G

Q

I

N

S

K

T

D

F

Y

V

V

G

-

E

-

E

E

L

V

A

G

K

G

S

G

D

N

R

D

P

I

E

D

L

I

T

T

A

T

A

V

K

D

I

F

V

I

V

M

S

S

G

I

G
|
G

R
|
R

G
|
G

M

I

Q

G

N

E

G

E

D

T

N

N

F

V

K

E

H

Q

L

F

Y

R

A

E

L

L

A

A

D

D

K

E

L

A

G

G

A

A

A

T

V

L

G

C

A

C

S
|
S

R
x
A

A
x
P

A

I

V

A

D

D

A

A

G

G

F

W

V

L

P

P

N

K

D

S

M

R

Q
|
Q

V
|
V

G
|
G

Q
|
Q

T
x
S

G

G

K

K

I

V

V

V

-

G

A

S

P

C

Q

K

L

L

Y

Y

I

V

A

A

V

M

G
|
G

I
|
I

S
|
S

G

G

A
x
S

I

I

Q
|
Q

H
|
H

L

M

A

A

G

G

M

M

K

K

D

H

S

V

K

P

V

T

I

I

V

I

A

A

I

V

N
|
N

K
x
T

D

D

E

P

E

G

A

A

P

S

I

I

F

F

Q

T

V

I

A

A

D

K

Y

Y

G

G

L

I

V

V

A

A

D
|
D

L
x
I

F

F

E

D

A

I

I

E

P

E

E

E

M

L

A

K

A

A

binding flavin-adenine dinucleotide: G209 (= G210), R210 (= R211), G211 (= G212), S235 (= S236), A236 (≠ R237), P237 (≠ A238), Q249 (= Q250), V250 (= V251), G251 (= G252), Q252 (= Q253), S253 (≠ T254), G267 (= G267), I268 (= I268), S269 (= S269), S271 (≠ A271), Q273 (= Q273), H274 (= H274), N288 (= N288), T289 (≠ K289), D306 (= D306), I307 (≠ L307)

Query Sequence

>Pf1N1B4_5613 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5613
MTILVIADYSKDAQDNKVLAPATLNTVAAAAKIGGDIHVLVAGAFVGSVAEAAAKIAGVA
KVLVADNAAYAYQLPENVAPLVAGLVIEQRTIYSHVLASATSNGKNILPRVAAQLDADQI
SEIISVESADTFKRPIYAGNAIATVQSNAAVKVITVRATGFDPVAAEGGSAAVEAVAAAH
DAGISTFVGEELAKSDRPELTAAKIVVSGGRGMQNGDNFKHLYALADKLGAAVGASRAAV
DAGFVPNDMQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPIFQVAD
YGLVADLFEAIPEMAALA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory