SitesBLAST – Find functional sites

 

SitesBLAST

Comparing Pf1N1B4_5765 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5765 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
82% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 1cchA

query
sites
1cchA
Q
 
Q
D
 
D
G
 
G
Q
 
E
E
 
A
L
 
L
F
 
F
K
 
K
S
 
S
K
 
K
P
 
P
C
|
C
A
 
A
A
 
A
C
|
C
H
|
H
S
 
S
I
 
V
D
 
D
T
 
T
K
 
K
L
 
M
V
 
V
G
 
G
P
 
P
A
 
A
L
 
L
K
 
K
D
 
E
V
 
V
A
 
A
A
 
A
K
 
K
N
 
N
A
 
A
G
 
G
V
 
V
A
 
E
G
 
G
A
 
A
V
 
A
D
 
D
T
 
T
L
|
L
A
 
A
S
 
L
H
|
H
I
|
I
K
 
K
N
 
N
G
 
G
T
x
S
Q
|
Q
G
 
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
I
P
 
P
M
|
M
P
|
P
A
 
P
N
|
N
P
 
P
V
 
V
T
 
T
D
 
E
E
 
E
E
 
E
A
 
A
K
 
K
T
 
I
L
 
L
A
 
A
T
 
E
W
 
W
V
 
V
L
 
L
S
 
S
L
 
L
K
 
K

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
82% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 6kq1A

query
sites
6kq1A
Q
 
Q
D
|
D
G
 
G
Q
x
E
E
 
A
L
 
L
F
 
F
K
 
K
S
 
S
K
|
K
P
 
P
C
|
C
A
 
A
A
 
A
C
|
C
H
|
H
S
 
S
I
 
I
D
 
D
T
 
A
K
 
K
L
 
M
V
 
V
G
|
G
P
|
P
A
 
A
L
 
L
K
 
K
D
 
E
V
|
V
A
 
A
A
 
A
K
 
K
N
x
Y
A
 
A
G
 
G
V
 
Q
A
 
E
G
 
G
A
 
A
V
 
A
D
 
D
T
 
L
L
|
L
A
 
A
S
 
G
H
|
H
I
|
I
K
 
K
N
 
N
G
 
G
T
 
T
Q
|
Q
G
|
G
N
|
N
W
|
W
G
|
G
P
 
P
M
x
I
P
 
P
M
|
M
P
 
P
A
 
P
N
|
N
P
 
P
V
 
V
T
 
T
D
 
E
E
 
E
E
|
E
A
 
A
K
 
K
T
 
T
L
 
L
A
 
A
T
 
E
W
 
W
V
 
V
L
 
L
S
 
S
L
 
L
K
 
K

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
76% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 1corA

query
sites
1corA
Q
 
Q
D
 
D
G
 
G
Q
 
E
E
 
A
L
 
L
F
 
F
K
 
K
S
 
S
K
|
K
P
 
P
C
|
C
A
 
A
A
 
A
C
|
C
H
|
H
S
 
S
I
 
I
D
 
D
T
 
A
K
 
K
L
 
L
V
|
V
G
|
G
P
|
P
A
 
A
L
 
F
K
 
K
D
 
E
V
 
V
A
 
A
A
 
A
K
 
K
N
 
Y
A
 
A
G
 
G
V
 
Q
A
 
D
G
 
G
A
 
A
V
 
A
D
 
D
T
 
L
L
|
L
A
 
A
S
 
G
H
|
H
I
|
I
K
 
K
N
 
N
G
 
G
T
x
S
Q
|
Q
G
|
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
I
P
 
P
M
|
M
P
 
P
A
 
P
N
|
N
P
 
P
V
 
V
T
 
T
D
 
E
E
 
E
E
 
E
A
 
A
K
 
K
T
 
I
L
 
L
A
 
A
T
 
E
W
 
W
V
 
I
L
 
L
S
 
S
L
 
Q
K
 
K

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
64% identity, 96% coverage: 5:104/104 of query aligns to 6:104/104 of P00099

query
sites
P00099
L
|
L
F
x
L
S
|
S
L
|
L
F
x
L
A
|
A
L
 
-
T
|
T
A
x
G
A
x
T
L
|
L
S
x
L
V
x
A
Q
|
Q
P
x
G
A
|
A
M
x
W
A
|
A
Q
x
E
D
|
D
G
x
P
Q
x
E
E
x
V
L
|
L
F
|
F
K
|
K
S
x
N
K
|
K
P
x
G
C
|
C
A
x
V
A
|
A
C
|
C
H
|
H
S
x
A
I
|
I
D
|
D
T
|
T
K
|
K
L
x
M
V
|
V
G
|
G
P
|
P
A
|
A
L
x
Y
K
|
K
D
|
D
V
|
V
A
|
A
A
|
A
K
|
K
N
x
F
A
|
A
G
|
G
V
x
Q
A
|
A
G
|
G
A
|
A
V
x
E
D
x
A
T
x
E
L
|
L
A
|
A
S
x
Q
H
x
R
I
|
I
K
|
K
N
|
N
G
|
G
T
x
S
Q
|
Q
G
|
G
N
x
V
W
|
W
G
|
G
P
|
P
M
x
I
P
|
P
M
|
M
P
|
P
A
x
P
N
|
N
P
x
A
V
|
V
T
x
S
D
|
D
E
x
D
E
|
E
A
|
A
K
x
Q
T
|
T
L
|
L
A
|
A
T
x
K
W
|
W
V
|
V
L
|
L
S
|
S
L
x
Q
K
|
K

Sites not aligning to the query:

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
67% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 351cA

query
sites
351cA
Q
 
E
D
 
D
G
 
P
Q
 
E
E
 
V
L
 
L
F
 
F
K
 
K
S
 
N
K
 
K
P
 
G
C
|
C
A
 
V
A
 
A
C
|
C
H
|
H
S
 
A
I
 
I
D
 
D
T
 
T
K
 
K
L
 
M
V
 
V
G
|
G
P
|
P
A
 
A
L
x
Y
K
 
K
D
 
D
V
 
V
A
 
A
A
 
A
K
 
K
N
 
F
A
 
A
G
 
G
V
 
Q
A
 
A
G
 
G
A
 
A
V
 
E
D
 
A
T
 
E
L
|
L
A
 
A
S
 
Q
H
x
R
I
|
I
K
 
K
N
 
N
G
 
G
T
 
S
Q
|
Q
G
|
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
I
P
|
P
M
|
M
P
 
P
A
 
P
N
 
N
P
 
A
V
 
V
T
 
S
D
 
D
E
 
D
E
 
E
A
 
A
K
 
Q
T
 
T
L
 
L
A
 
A
T
 
K
W
 
W
V
 
V
L
 
L
S
 
S
L
 
Q
K
 
K

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
66% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 2exvA

query
sites
2exvA
Q
 
E
D
 
D
G
 
P
Q
 
E
E
 
V
L
 
L
F
 
A
K
 
K
S
 
N
K
 
K
P
 
G
C
|
C
A
 
V
A
 
A
C
|
C
H
|
H
S
 
A
I
 
I
D
 
D
T
 
T
K
 
K
L
 
M
V
 
V
G
|
G
P
|
P
A
 
A
L
 
Y
K
 
K
D
 
D
V
 
V
A
 
A
A
 
A
K
 
K
N
 
F
A
 
A
G
 
G
V
 
Q
A
 
A
G
 
G
A
 
A
V
 
E
D
 
A
T
 
E
L
 
L
A
 
A
S
 
Q
H
x
R
I
|
I
K
 
K
N
 
N
G
 
G
T
x
S
Q
|
Q
G
|
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
I
P
|
P
M
|
M
P
 
P
A
 
P
N
|
N
P
 
A
V
 
V
T
 
S
D
 
D
E
 
D
E
 
E
A
 
A
K
 
Q
T
 
T
L
 
L
A
 
A
T
 
K
W
 
W
V
 
V
L
 
L
S
 
S
L
 
Q
K
 
K

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
63% identity, 76% coverage: 26:104/104 of query aligns to 2:80/80 of 5xecC

query
sites
5xecC
Q
 
E
E
 
Q
L
 
L
F
 
A
K
 
K
S
 
Q
K
 
K
P
 
G
C
|
C
A
 
M
A
 
A
C
|
C
H
|
H
S
 
D
I
 
L
D
 
K
T
 
A
K
 
K
L
 
M
V
 
V
G
 
G
P
|
P
A
 
A
L
x
Y
K
 
K
D
 
D
V
 
V
A
 
A
A
 
A
K
 
K
N
 
F
A
 
A
G
 
G
V
 
Q
A
 
A
G
 
G
A
 
A
V
 
E
D
 
A
T
 
E
L
|
L
A
 
A
S
 
Q
H
x
R
I
 
I
K
 
K
N
 
N
G
 
G
T
 
S
Q
|
Q
G
 
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
I
P
 
P
M
|
M
P
 
P
A
 
P
N
|
N
P
 
A
V
 
V
T
 
S
D
 
D
E
 
D
E
 
E
A
 
A
K
 
Q
T
 
T
L
 
L
A
 
A
T
 
K
W
 
W
V
 
V
L
 
L
S
 
S
L
 
Q
K
 
K

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
60% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 5xecA

query
sites
5xecA
Q
 
E
D
 
D
G
 
P
Q
 
E
E
 
V
L
 
L
F
 
F
K
 
K
S
 
N
K
 
K
P
 
G
C
|
C
A
 
V
A
 
A
C
|
C
H
|
H
S
 
A
I
 
I
D
 
D
T
 
T
K
 
K
L
 
K
V
 
V
G
|
G
P
|
P
A
 
A
L
x
Y
K
 
A
D
 
D
V
 
V
A
 
A
A
 
K
K
 
K
N
x
Y
A
 
A
G
 
G
V
 
R
A
 
K
G
 
D
A
 
A
V
 
V
D
 
D
T
x
Y
L
 
L
A
 
A
S
 
G
H
 
K
I
|
I
K
 
K
N
 
K
G
 
G
T
 
G
Q
x
S
G
|
G
N
x
V
W
|
W
G
|
G
P
 
S
M
x
V
P
 
P
M
|
M
P
 
P
A
 
P
N
x
Q
P
 
N
V
 
V
T
 
T
D
 
D
E
 
A
E
 
E
A
 
A
K
 
K
T
 
Q
L
 
L
A
 
A
T
 
Q
W
 
W
V
x
I
L
 
L
S
 
S
L
 
I
K
 
K

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
54% identity, 76% coverage: 26:104/104 of query aligns to 2:83/83 of 5aurA

query
sites
5aurA
Q
 
E
E
 
Q
L
 
L
F
 
A
K
 
K
S
 
Q
K
 
K
P
 
G
C
|
C
A
 
M
A
 
A
C
|
C
H
|
H
S
 
D
I
 
L
D
 
K
T
 
A
-
 
G
-
 
G
-
 
G
K
 
K
L
 
K
V
 
V
G
 
G
P
|
P
A
 
A
L
x
Y
K
 
A
D
 
D
V
 
V
A
 
A
A
 
K
K
 
K
N
x
Y
A
 
A
G
 
G
V
 
R
A
 
K
G
 
D
A
 
A
V
 
V
D
 
D
T
x
Y
L
 
L
A
 
A
S
 
G
H
 
K
I
|
I
K
 
K
N
 
K
G
 
G
T
 
G
Q
x
S
G
|
G
N
x
V
W
|
W
G
|
G
P
 
S
M
x
V
P
|
P
M
|
M
P
 
P
A
 
P
N
x
Q
P
 
N
V
 
V
T
 
T
D
 
D
E
 
A
E
 
E
A
 
A
K
 
K
T
 
Q
L
 
L
A
 
A
T
 
Q
W
 
W
V
 
I
L
 
L
S
 
S
L
 
I
K
 
K

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
53% identity, 75% coverage: 27:104/104 of query aligns to 3:81/81 of 1a56A

query
sites
1a56A
E
 
D
L
 
L
F
 
A
K
 
K
S
 
K
K
 
N
P
x
N
C
|
C
A
 
I
A
 
A
C
|
C
H
|
H
S
 
Q
I
 
V
D
 
E
T
 
T
K
 
K
L
 
V
V
|
V
G
|
G
P
|
P
A
 
A
L
 
L
K
 
K
D
 
D
V
 
I
A
 
A
A
 
A
K
 
K
N
 
Y
A
 
A
G
 
D
V
 
K
A
 
D
G
 
D
A
 
A
V
 
A
D
 
T
T
 
Y
L
 
L
A
 
A
S
 
G
H
x
K
I
|
I
K
 
K
N
 
G
G
 
G
T
x
S
Q
 
S
G
 
G
N
 
V
W
|
W
G
 
G
P
x
Q
M
x
I
P
 
P
M
|
M
P
 
P
A
 
P
N
|
N
-
x
V
P
x
N
V
 
V
T
 
S
D
 
D
E
 
A
E
 
D
A
 
A
K
 
K
T
 
A
L
 
L
A
 
A
T
 
D
W
 
W
V
 
I
L
 
L
S
 
T
L
 
L
K
 
K

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
46% identity, 76% coverage: 26:104/104 of query aligns to 2:79/79 of 2d0sA

query
sites
2d0sA
Q
 
E
E
 
A
L
 
L
F
 
A
K
 
K
S
 
A
K
 
K
P
 
G
C
|
C
A
 
M
A
 
A
C
|
C
H
|
H
S
 
A
I
 
I
D
 
D
T
 
K
K
 
K
L
 
L
V
 
V
G
 
G
P
|
P
A
 
S
L
 
Y
K
 
K
D
 
D
V
 
V
A
 
A
A
 
K
K
 
K
N
x
Y
A
 
T
G
 
-
V
 
-
A
 
E
G
 
A
A
 
D
V
 
V
D
 
P
T
 
K
L
 
L
A
 
V
S
 
E
H
x
K
I
x
V
K
 
K
N
 
K
G
 
G
T
 
G
Q
x
A
G
|
G
N
x
V
W
|
W
G
|
G
P
 
P
M
x
V
P
 
P
M
|
M
P
|
P
A
 
P
N
x
H
P
 
P
-
 
Q
V
 
V
T
 
A
D
 
E
E
 
A
E
 
D
A
 
I
K
 
E
T
 
K
L
 
I
A
 
V
T
 
R
W
 
W
V
 
V
L
 
L
S
 
T
L
 
L
K
 
K

6cunA Engineered cytochromE C from rhodothermus marinus (rma tde) bound to carbene intermediate (1-ethoxy-1-oxopropan-2-ylidene) (see paper)
41% identity, 42% coverage: 16:59/104 of query aligns to 20:62/112 of 6cunA

query
sites
6cunA
S
 
Q
V
 
I
Q
 
D
P
 
A
A
 
A
M
 
L
A
 
A
Q
 
Q
D
 
Q
G
 
G
Q
 
E
E
 
Q
L
 
L
F
 
F
K
 
N
S
 
T
K
 
Y
P
 
-
C
|
C
A
 
T
A
 
A
C
|
C
H
|
H
S
 
R
I
 
L
D
 
D
T
 
E
K
 
R
L
 
F
V
 
I
G
 
G
P
|
P
A
 
A
L
 
L
K
 
R
D
 
D
V
 
V
A
 
T
A
 
K
K
x
R
N
x
R
A
 
G
G
 
P
V
 
V

Sites not aligning to the query:

3cp5A CytochromE C from rhodothermus marinus (see paper)
41% identity, 42% coverage: 16:59/104 of query aligns to 20:62/116 of 3cp5A

query
sites
3cp5A
S
 
Q
V
 
I
Q
 
D
P
 
A
A
 
A
M
 
L
A
 
A
Q
 
Q
D
 
Q
G
 
G
Q
 
E
E
 
Q
L
 
L
F
 
F
K
 
N
S
 
T
K
x
Y
P
 
-
C
|
C
A
 
T
A
 
A
C
|
C
H
|
H
S
 
R
I
 
L
D
 
D
T
 
E
K
 
R
L
 
F
V
 
I
G
|
G
P
|
P
A
 
A
L
 
L
K
 
R
D
 
D
V
 
V
A
 
T
A
 
K
K
x
R
N
x
R
A
 
G
G
 
P
V
 
V

Sites not aligning to the query:

3x15A Dimeric aquifex aeolicus cytochrome c555 (see paper)
30% identity, 78% coverage: 24:104/104 of query aligns to 2:87/87 of 3x15A

query
sites
3x15A
D
 
D
G
 
G
Q
 
K
E
 
A
L
 
I
F
 
F
K
 
Q
S
 
Q
K
 
K
P
 
G
C
|
C
A
 
G
A
 
S
C
|
C
H
|
H
S
 
Q
I
 
A
D
 
N
T
 
V
K
 
D
L
 
T
V
 
V
G
 
G
P
|
P
A
 
S
L
 
L
K
 
K
D
 
K
V
 
I
A
 
A
A
 
-
K
 
-
N
 
-
A
 
Q
G
 
A
V
x
Y
A
 
A
G
 
G
A
 
K
V
 
E
D
 
D
T
 
Q
L
 
L
A
 
I
S
 
K
H
x
F
I
 
L
K
 
K
N
 
G
-
 
E
-
 
A
-
 
P
-
x
A
-
x
I
-
x
V
-
 
D
-
 
P
G
 
A
T
x
K
Q
x
E
G
 
A
N
 
I
W
x
M
G
 
K
P
 
P
M
x
Q
P
 
L
M
 
T
P
 
M
A
 
L
N
 
K
P
 
G
V
 
L
T
 
S
D
 
D
E
 
A
E
 
E
A
 
L
K
 
K
T
 
A
L
 
L
A
 
A
T
 
D
W
 
F
V
 
I
L
 
L
S
 
S
L
 
H
K
 
K

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
31% identity, 75% coverage: 26:103/104 of query aligns to 8:90/92 of 4xxlA

query
sites
4xxlA
Q
 
K
E
 
S
L
 
L
F
 
A
K
 
R
S
 
E
K
 
N
P
 
N
C
|
C
A
 
F
A
 
K
C
|
C
H
|
H
S
 
G
I
 
V
D
 
D
T
 
K
K
 
E
L
 
K
V
 
D
G
|
G
P
|
P
A
 
S
L
x
Y
K
 
K
D
 
K
V
 
V
A
 
A
A
 
E
K
|
K
N
x
Y
A
 
R
G
 
G
V
 
K
A
 
A
G
 
D
A
 
A
V
 
E
D
 
A
T
 
K
L
 
L
A
 
I
S
 
H
H
|
H
I
 
V
K
 
T
N
 
S
G
 
G
T
 
E
Q
 
K
G
x
A
N
x
K
W
 
F
-
 
P
-
 
D
G
 
G
P
 
H
M
 
E
P
 
E
M
 
E
P
x
H
A
 
K
N
 
N
-
 
I
-
 
N
-
 
G
P
 
K
V
 
A
T
 
S
D
 
P
E
 
E
E
 
A
A
 
I
K
 
K
T
 
N
L
 
L
A
 
V
T
 
D
W
 
W
V
 
I
L
 
L
S
 
S
L
 
L

Query Sequence

>Pf1N1B4_5765 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5765
MKNTLFSLFALTAALSVQPAMAQDGQELFKSKPCAACHSIDTKLVGPALKDVAAKNAGVA
GAVDTLASHIKNGTQGNWGPMPMPANPVTDEEAKTLATWVLSLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory