SitesBLAST

P00099 Cytochrome c-551; Cytochrome C8; Cytochrome c551 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
64% identity, 96% coverage: 5:104/104 of query aligns to 6:104/104 of P00099

query
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P00099

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23:104 (vs. 23:104, 67% identical) modified: mature protein, Cytochrome c-551
H38 (= H38) binding axial binding residue
M83 (= M83) binding axial binding residue

Sites not aligning to the query:

1:22 signal peptide

351cA Structure of cytochrome c551 from p. Aeruginosa refined at 1.6 angstroms resolution and comparison of the two redox forms (see paper)
67% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 351cA

query
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351cA

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binding protoporphyrin ix containing fe: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), Y27 (≠ L49), L44 (= L66), R47 (≠ H69), I48 (= I70), Q53 (= Q75), G54 (= G76), V55 (≠ N77), W56 (= W78), G57 (= G79), I59 (≠ M81), P60 (= P82), M61 (= M83)

2exvA Crystal structure of the f7a mutant of the cytochrome c551 from pseudomonas aeruginosa (see paper)
66% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 2exvA

query
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2exvA

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binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), R47 (≠ H69), I48 (= I70), S52 (≠ T74), Q53 (= Q75), G54 (= G76), V55 (≠ N77), W56 (= W78), G57 (= G79), I59 (≠ M81), P60 (= P82), M61 (= M83), N64 (= N86)

5xecC Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
63% identity, 76% coverage: 26:104/104 of query aligns to 2:80/80 of 5xecC

query
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5xecC

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binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P23 (= P47), Y25 (≠ L49), L42 (= L66), R45 (≠ H69), Q51 (= Q75), V53 (≠ N77), W54 (= W78), G55 (= G79), I57 (≠ M81), M59 (= M83), N62 (= N86)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
60% identity, 79% coverage: 23:104/104 of query aligns to 1:82/82 of 5xecA

query
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5xecA

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binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), G24 (= G46), P25 (= P47), Y27 (≠ L49), Y34 (≠ N56), Y43 (≠ T65), I48 (= I70), S53 (≠ Q75), G54 (= G76), V55 (≠ N77), W56 (= W78), G57 (= G79), V59 (≠ M81), M61 (= M83), Q64 (≠ N86), I78 (≠ V100)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
54% identity, 76% coverage: 26:104/104 of query aligns to 2:83/83 of 5aurA

query
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5aurA

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binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P26 (= P47), Y28 (≠ L49), Y35 (≠ N56), Y44 (≠ T65), I49 (= I70), S54 (≠ Q75), G55 (= G76), V56 (≠ N77), W57 (= W78), G58 (= G79), V60 (≠ M81), P61 (= P82), M62 (= M83), Q65 (≠ N86)

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
53% identity, 75% coverage: 27:104/104 of query aligns to 3:81/81 of 1a56A

query
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1a56A

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binding heme c: N9 (≠ P33), C10 (= C34), C13 (= C37), H14 (= H38), V21 (= V45), G22 (= G46), P23 (= P47), K45 (≠ H69), I46 (= I70), S50 (≠ T74), W54 (= W78), Q56 (≠ P80), I57 (≠ M81), M59 (= M83), N62 (= N86), V63 (vs. gap), N64 (≠ P87)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
46% identity, 76% coverage: 26:104/104 of query aligns to 2:79/79 of 2d0sA

query
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2d0sA

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binding heme c: C10 (= C34), C13 (= C37), H14 (= H38), P23 (= P47), Y32 (≠ N56), K43 (≠ H69), V44 (≠ I70), A49 (≠ Q75), G50 (= G76), V51 (≠ N77), W52 (= W78), G53 (= G79), V55 (≠ M81), M57 (= M83), P58 (= P84), H60 (≠ N86)

6cunA Engineered cytochromE C from rhodothermus marinus (rma tde) bound to carbene intermediate (1-ethoxy-1-oxopropan-2-ylidene) (see paper)
41% identity, 42% coverage: 16:59/104 of query aligns to 20:62/112 of 6cunA

query
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6cunA

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binding heme c: C37 (= C34), C40 (= C37), H41 (= H38), P50 (= P47), R58 (≠ K55), R59 (≠ N56)

Sites not aligning to the query:

binding ethyl propionate: 67, 71, 75, 81
binding heme c: 63, 67, 84, 85, 89, 102

3cp5A CytochromE C from rhodothermus marinus (see paper)
41% identity, 42% coverage: 16:59/104 of query aligns to 20:62/116 of 3cp5A

query
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3cp5A

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binding heme c: Y36 (≠ K32), C37 (= C34), C40 (= C37), H41 (= H38), G49 (= G46), P50 (= P47), R58 (≠ K55), R59 (≠ N56)

Sites not aligning to the query:

binding heme c: 63, 67, 68, 81, 84, 85, 90, 91, 92, 105

3x15A Dimeric aquifex aeolicus cytochrome c555 (see paper)
30% identity, 78% coverage: 24:104/104 of query aligns to 2:87/87 of 3x15A

query
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3x15A

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binding heme c: C12 (= C34), C15 (= C37), H16 (= H38), P25 (= P47), Y34 (≠ V59), F44 (≠ H69), A51 (vs. gap), I52 (vs. gap), V53 (vs. gap), K57 (≠ T74), E58 (≠ Q75), M61 (≠ W78), Q64 (≠ M81)

4xxlA Crystal structure of class 1 cytochrome mtod from sideroxydans lithotrophicus es-1 (see paper)
31% identity, 75% coverage: 26:103/104 of query aligns to 8:90/92 of 4xxlA

query
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4xxlA

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A

N
x
K

W

F

-

P

-

D

G

G

P

H

M

E

P

E

M

E

P
x
H

A

K

N

N

-

I

-

N

-

G

P

K

V

A

T

S

D

P

E

E

E

A

A

I

K

K

T

N

L

L

A

V

T

D

W

W

V

I

L

L

S

S

L

L

binding heme c: C16 (= C34), C19 (= C37), H20 (= H38), G28 (= G46), P29 (= P47), Y31 (≠ L49), K37 (= K55), Y38 (≠ N56), H51 (= H69), A58 (≠ G76), K59 (≠ N77), H68 (≠ P84)

Query Sequence

>Pf1N1B4_5765 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5765
MKNTLFSLFALTAALSVQPAMAQDGQELFKSKPCAACHSIDTKLVGPALKDVAAKNAGVA
GAVDTLASHIKNGTQGNWGPMPMPANPVTDEEAKTLATWVLSLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory