SitesBLAST
Comparing Pf1N1B4_6047 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_6047 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 8 hits to proteins with known functional sites (download)
7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
22% identity, 84% coverage: 31:388/427 of query aligns to 3:349/363 of 7cyxA
- binding flavin-adenine dinucleotide: I7 (= I35), G8 (= G36), G9 (≠ A37), G10 (= G38), V11 (≠ Y39), I12 (≠ T40), V30 (≠ L58), E31 (= E59), K32 (≠ A60), E38 (≠ G66), A39 (= A67), S40 (= S68), A42 (≠ R70), A43 (≠ N71), A44 (≠ G72), G45 (= G73), L46 (≠ Q74), T169 (≠ S207), E170 (≠ P208), V171 (≠ A209), A199 (= A235), G200 (= G236), G201 (≠ N237), W203 (≠ Y239), Y244 (≠ C278), G298 (= G339), R300 (≠ F341), P301 (≠ L342), H325 (= H367), Y326 (vs. gap), R327 (vs. gap), N328 (vs. gap), G329 (= G368), I330 (≠ V369), L331 (≠ T370)
1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
21% identity, 62% coverage: 124:386/427 of query aligns to 92:349/364 of 1ng3A
- binding acetylamino-acetic acid: Y241 (≠ L273), Y246 (= Y288), R302 (≠ F341), R329 (≠ G366)
- binding flavin-adenine dinucleotide: P173 (= P208), V174 (≠ A209), A201 (= A235), S202 (≠ G236), G203 (≠ N237), W205 (≠ Y239), F209 (≠ L243), G224 (≠ T256), Y246 (= Y288), G300 (= G339), R302 (≠ F341), H327 (≠ C364), F328 (≠ S365), R329 (≠ G366), N330 (≠ H367), G331 (= G368), I332 (≠ V369), L333 (≠ T370)
- binding phosphate ion: R254 (= R296)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding acetylamino-acetic acid: 49
- binding flavin-adenine dinucleotide: 10, 11, 12, 13, 14, 15, 33, 34, 35, 41, 42, 43, 45, 46, 47, 48, 49
- binding phosphate ion: 89
O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
21% identity, 62% coverage: 124:386/427 of query aligns to 92:349/369 of O31616
- V174 (≠ A209) binding
- H244 (≠ Y277) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
- R302 (≠ F341) binding
- 327:333 (vs. 364:370, 14% identical) binding
- R329 (≠ G366) binding
Sites not aligning to the query:
- 14:15 binding
- 34:35 binding
- 42:43 binding
- 47:49 binding
- 51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
- 54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
21% identity, 62% coverage: 124:386/427 of query aligns to 92:349/364 of 3if9A
- binding flavin-adenine dinucleotide: P173 (= P208), V174 (≠ A209), A201 (= A235), S202 (≠ G236), G203 (≠ N237), W205 (≠ Y239), F209 (≠ L243), G224 (≠ T256), Y246 (= Y288), G300 (= G339), R302 (≠ F341), P303 (≠ L342), H327 (≠ C364), F328 (≠ S365), R329 (≠ G366), N330 (≠ H367), G331 (= G368), I332 (≠ V369), L333 (≠ T370)
- binding glycolic acid: Y246 (= Y288), R302 (≠ F341), R329 (≠ G366)
Sites not aligning to the query:
- active site: 47, 48, 49
- binding flavin-adenine dinucleotide: 10, 11, 12, 13, 14, 15, 33, 34, 35, 41, 42, 43, 45, 46, 47, 48, 49
Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
22% identity, 63% coverage: 18:285/427 of query aligns to 32:293/857 of Q63342
- CV 52:53 (≠ YT 39:40) binding
- EK 73:74 (≠ EA 59:60) binding
- 80:88 (vs. 66:75, 20% identical) binding
- H84 (≠ R70) modified: Tele-8alpha-FAD histidine
- V212 (≠ A209) binding
- W244 (≠ Y239) binding
Sites not aligning to the query:
- 390:395 binding
- 573:575 binding
- 669 binding
- 676:678 binding
- 737 binding
5i39A High resolution structure of l-amino acid deaminase from proteus vulgaris with the deletion of the specific insertion sequence (see paper)
22% identity, 85% coverage: 29:391/427 of query aligns to 24:374/383 of 5i39A
- active site: F66 (≠ N71), Q69 (= Q74), A70 (≠ I75), Q248 (≠ S251), P267 (= P274)
- binding flavin-adenine dinucleotide: V30 (≠ I35), G31 (= G36), G33 (= G38), I34 (≠ Y39), L35 (≠ T40), V53 (≠ L58), E54 (= E59), K55 (≠ A60), E61 (≠ G66), Q62 (≠ A67), S63 (= S68), R65 (= R70), F66 (≠ N71), Y67 (≠ G72), G68 (= G73), Q69 (= Q74), A196 (≠ P208), A197 (= A209), A225 (= A235), G226 (= G236), G227 (≠ N237), W229 (vs. gap), F233 (≠ Y239), Q248 (≠ S251), Q250 (≠ T256), G321 (= G339), A322 (≠ N340), M323 (≠ F341), T348 (≠ S365), G349 (= G366), W350 (≠ H367), G351 (= G368), M352 (≠ V369), T353 (= T370)
4pabB Crystal structure of the precursor form of rat dmgdh complexed with tetrahydrofolate (see paper)
23% identity, 61% coverage: 25:285/427 of query aligns to 3:256/824 of 4pabB
- active site: T53 (≠ N77), E102 (≠ H138), H226 (≠ T256), Y255 (= Y284)
- binding flavin-adenine dinucleotide: I11 (= I35), G12 (= G36), G13 (≠ A37), G14 (= G38), C15 (≠ Y39), V16 (≠ T40), L35 (= L58), E36 (= E59), K37 (≠ A60), A42 (≠ F65), G43 (= G66), S44 (≠ A67), T45 (≠ S68), H47 (≠ R70), A48 (≠ N71), A49 (≠ G72), G50 (= G73), L51 (≠ I75), P174 (= P208), V175 (≠ A209), A203 (= A235), A204 (≠ G236), G205 (≠ N237), F206 (≠ A238), W207 (≠ Y239), H226 (≠ T256), Y228 (≠ V258)
Sites not aligning to the query:
- active site: 536
- binding flavin-adenine dinucleotide: 324, 326, 327, 328, 353, 354, 355, 356, 357, 358
- binding (6s)-5,6,7,8-tetrahydrofolate: 493, 523, 536, 538, 550, 612, 613, 630, 632, 639, 641, 680, 700, 701
Q6TGQ9 L-amino-acid oxidase BjussuLAAO-I; BjsuLAAO; LAO; EC 1.4.3.2 from Bothrops jararacussu (Jararacussu) (see paper)
36% identity, 21% coverage: 32:121/427 of query aligns to 49:123/497 of Q6TGQ9
Sites not aligning to the query:
- 23 modified: Disulfide link with 186
- 186 modified: Disulfide link with 23
- 344 modified: Disulfide link with 425
- 425 modified: Disulfide link with 344
Query Sequence
>Pf1N1B4_6047 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_6047
MANTPYPESYYAASANVAPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEA
AKVGFGASGRNGGQIVNSYSRDIDVIERSVGPKQAQLLGQMAFEGGRIIRERVAKYNIQC
DLKDGGVFAAITAKQMGHLESQKRLWERFGHTQLELLDQRRIREVVACDQYVGGMLDMSG
GHIHPLNLVLGEAAAVESLGGTIYEQSPAVRIERGTNPVVYTPQGKVRAKFIIVAGNAYI
GNLVPELAAKSMPCGTQVIATEPLGDELAKRLLPQDYCVEDCNYLLDYYRLSADKRLIFG
GGVVYGARDPANIEAIIRPKMLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAALGAWYYG
LRDKLGF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory