SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf1N1B4_925 FitnessBrowser__pseudo1_N1B4:Pf1N1B4_925 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3ozwA The crystal structure of flavohemoglobin from r. Eutrophus in complex with ketoconazole (see paper)
43% identity, 99% coverage: 1:388/393 of query aligns to 1:398/403 of 3ozwA

query
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3ozwA

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binding flavin-adenine dinucleotide: N44 (= N44), A46 (= A46), E49 (≠ A49), Q50 (≠ S50), K84 (= K84), Y190 (= Y191), R206 (= R205), Q207 (≠ N206), Y208 (= Y207), S209 (= S208), S222 (= S221), V223 (= V222), K224 (= K223), E226 (= E225), Q231 (vs. gap), P232 (vs. gap), G234 (= G228), Y235 (≠ R229), V236 (≠ A230), S237 (= S231), V276 (= V269), T279 (= T272), E394 (= E384), V395 (≠ F385), F396 (= F386), G397 (= G387)
binding protoporphyrin ix containing fe: V42 (≠ L42), F43 (= F43), N44 (= N44), I81 (= I81), K84 (= K84), H85 (= H85), L88 (= L88), V90 (≠ I90), Q94 (≠ H94), Y95 (= Y95), V98 (= V98), Y126 (= Y127), L129 (= L130), A130 (= A131), L133 (= L134), P398 (= P388)
binding 1-acetyl-4-(4-{[(2R,4S)-2-(2,4-dichlorophenyl)-2-(1H-imidazol-1-ylmethyl)-1,3-dioxolan-4-yl]methoxy}phenyl)piperazine: I25 (= I25), Y29 (= Y29), N44 (= N44), Q50 (≠ S50), Q53 (= Q53), A56 (= A56), L57 (= L57), L102 (= L102), P398 (= P388)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 399

3ozvA The crystal structure of flavohemoglobin from r. Eutrophus in complex with econazole (see paper)
43% identity, 99% coverage: 1:388/393 of query aligns to 1:398/403 of 3ozvA

query
sites
3ozvA

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binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: I24 (≠ L24), F28 (= F28), F43 (= F43), Q53 (= Q53), L57 (= L57), V61 (= V61), L102 (= L102), I106 (= I106)
binding flavin-adenine dinucleotide: N44 (= N44), A46 (= A46), Q48 (= Q48), K84 (= K84), Y190 (= Y191), R206 (= R205), Q207 (≠ N206), Y208 (= Y207), S209 (= S208), S222 (= S221), V223 (= V222), K224 (= K223), E226 (= E225), Q231 (vs. gap), P232 (vs. gap), P233 (vs. gap), G234 (= G228), Y235 (≠ R229), V236 (≠ A230), S237 (= S231), V276 (= V269), T279 (= T272), E394 (= E384), V395 (≠ F385), F396 (= F386), G397 (= G387)
binding protoporphyrin ix containing fe: V42 (≠ L42), F43 (= F43), N44 (= N44), H47 (= H47), Q53 (= Q53), I81 (= I81), K84 (= K84), H85 (= H85), L88 (= L88), V90 (≠ I90), Q94 (≠ H94), Y95 (= Y95), V98 (= V98), Y126 (= Y127), L129 (= L130), A130 (= A131), L133 (= L134), P398 (= P388)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 399

3ozuA The crystal structure of flavohemoglobin from r. Eutrophus in complex with miconazole (see paper)
43% identity, 99% coverage: 1:388/393 of query aligns to 1:398/403 of 3ozuA

query
sites
3ozuA

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R

P

Q

V

V

H

V

F

F

I

V

H

H

C

G

A

A

R

R

N

N

G

S

S

A

V

V

H

H

A

A

F

M

R

R

D

D

W

R

I

L

D

R

G

E

L

A

A

A

E

K

R

T

H

Y

P

E

Q

N

L

L

K

D

R

L

F

F

Y

V

C

F

Y

Y

A

D

E

Q

-

P

-

L

-

P

D

E

D

D

G

V

I

Q

S

G

P

R

A

D

A

Y

D

D

K

Y

V

P

G

G

L

L

L

V

S

D

Q

V

E

K

Q

Q

L

I

G

E

E

K

-

S

-

I

W

L

L

L

P

P

E

-

Q

-

R

-

D

D

V

A

D

D

A

Y

Y

Y

F

I

L

C

G

G

P

P

K

I

G

P

F

F

M

M

A

R

A

M

I

Q

K

H

R

D

H

A

L

L

K

K

A

N

L

L

G

G

V

I

P

H

E

E

K

A

Q

R

S

I

R

H

Y

Y

E
|
E

F
x
V

F

F

G
|
G

P
|
P

binding flavin-adenine dinucleotide: N44 (= N44), A46 (= A46), H47 (= H47), Y190 (= Y191), R206 (= R205), Q207 (≠ N206), Y208 (= Y207), S209 (= S208), S222 (= S221), V223 (= V222), K224 (= K223), E226 (= E225), G227 (≠ T226), Q231 (vs. gap), P232 (vs. gap), P233 (vs. gap), G234 (= G228), Y235 (≠ R229), V236 (≠ A230), S237 (= S231), E394 (= E384), V395 (≠ F385), G397 (= G387), P398 (= P388)
binding protoporphyrin ix containing fe: V42 (≠ L42), F43 (= F43), N44 (= N44), I81 (= I81), K84 (= K84), H85 (= H85), L88 (= L88), V90 (≠ I90), Q94 (≠ H94), Y95 (= Y95), V98 (= V98), Y126 (= Y127), L129 (= L130), A130 (= A131), L133 (= L134)
binding 1-[(2R)-2-[(2,4-dichlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1H-imidazole: I25 (= I25), F28 (= F28), F43 (= F43), A56 (= A56), L57 (= L57), L102 (= L102), W122 (= W123), A125 (= A126), Y126 (= Y127)

P39662 Flavohemoprotein; FHP; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) (Ralstonia eutropha) (see paper)
43% identity, 99% coverage: 1:388/393 of query aligns to 1:398/403 of P39662

query
sites
P39662

M

M

L

L

S

T

V

Q

Q

K

D

T

R

K

A

D

I

I

V

V

K

K

S

A

T

T

V

A

P

P

L

V

L

L

E

A

S

E

G

H

G

G

E

Y

A

D

L

I

I

I

T

K

H

C

F

F

Y

Y

R

Q

M

R

M

M

L

F

S

E

E

A

Y

H

P

P

E

E

V

L

R

K

P

N

L

V

F

F

N

N

Q

M

A

A

H

H

Q

Q

A

E

S

Q

G

G

D

Q

Q

Q

P

Q

R

Q

A

A

L

L

A

A

N

R

G
x
A

V

V

L

Y

M

A

Y

Y

A

A

R

E

H

N

I

I

D

E

Q

D

L

P

D

N

Q

S

L

L

G

M

D

A

L

V

V

L

A

K

K

N

I

I

I

A

N

N

K

K

H

H

V

A

A

S

L

L

Q

G

I

V

L

K

P

P

E

E

H

Q

Y

Y

P

P

I

I

V
|
V

G

G

S

E

C

H

L

L

L

L

R

A

A

A

I

I

S

K

E

E

V

V

L

L

G

G

E

N

E

A

I

-

A

A

T

T

P

D

E

D

V

I

M

I

S

S

A

A

W

W

G

A

A

Q

A

A

Y

Y

G

G

Q

N

L

L

A

A

E

D

I

V

L

L

I

M

G

G

A

M

E

E

A

S

S

E

I

L

Y

Y

D

E

Q

R

K

S

A

A

Q

E

A

Q

P

P

G

G

G

G

W

W

R

K

G

G

A

W

R

R

E

T

F

F

I

V

V

I

V

R

A

E

K

K

V

R

E

P

E

E

S

S

A

D

E

V

I

I

I

T

S

S

F

F

Y

I

F

L

E

E

P

P

A

A

D

D

K

G

G

G

P

P

I

V

L

V

A

N

A

F

E

E

P

P

G

G

Q

Q

Y

Y

I

T

G

S

M

V

K

A

L

I

I

D

L

V

D

P

-

A

-

L

G

G

E

L

E

Q

I

Q

R

I

R

R

N

Q

Y

Y

S

S

L

L

S

S

A

D

L

M

A

P

N

N

K

G

G

R

Q

S

Y

Y

R

R

I

I

S

S

V

V

K

K

R

R

E

E

T

G

G

G

-

G

-

P

-

Q

-

P

-

P

G

G

R

Y

A

V

S

S

N

N

H

L

L

L

H

H

D

D

Q

H

L

V

H

N

V

V

G

G

A

D

S

Q

I

V

L

K

L

L

F

A

P

A

P

P

A

Y

G

G

D

S

F

F

T

H

L

I

T

D

A

V

S

D

D

A

K

K

-

T

P

P

L

I

V

V

L

L

I

I

S

S

G

G

G

G

V

V

G

G

I

L

T

T

P

P

T

M

L

V

A

S

M

M

L

L

E

K

A

V

A

A

L

L

-

Q

A

A

T

P

E

P

R

R

P

Q

V

V

H

V

F

F

I

V

H

H

C

G

A

A

R

R

N

N

G

S

S

A

V

V

H

H

A

A

F

M

R

R

D

D

W

R

I

L

D

R

G

E

L

A

A

A

E

K

R

T

H

Y

P

E

Q

N

L

L

K

D

R

L

F

F

Y

V

C

F

Y

Y

A

D

E

Q

-

P

-

L

-

P

D

E

D

D

G

V

I

Q

S

G

P

R

A

D

A

Y

D

D

K

Y

V

P

G

G

L

L

L

V

S

D

Q

V

E

K

Q

Q

L

I

G

E

E

K

-

S

-

I

W

L

L

L

P

P

E

-

Q

-

R

-

D

D

V

A

D

D

A

Y

Y

Y

F

I

L

C

G

G

P

P

K

I

G

P

F

F

M

M

A

R

A

M

I

Q

K

H

R

D

H

A

L

L

K

K

A

N

L

L

G

G

V

I

P

H

E

E

K

A

Q

R

S

I

R

H

Y

Y

E

E

F

V

F

F

G

G

P

P

A60 (≠ G60) mutation to Y: Does not affect phospholipid-binding.
V98 (= V98) mutation to F: Blocks phospholipid-binding.

1cqxA Crystal structure of the flavohemoglobin from alcaligenes eutrophus at 1.75 a resolution (see paper)
43% identity, 99% coverage: 1:388/393 of query aligns to 1:398/403 of 1cqxA

query
sites
1cqxA

M

M

L

L

S

T

V

Q

Q

K

D

T

R

K

A

D

I

I

V

V

K

K

S

A

T

T

V

A

P

P

L

V

L

L

E

A

S

E

G

H

G

G

E

Y

A

D

L

I

I

I

T

K

H

C

F

F

Y

Y

R

Q

M

R

M

M

L

F

S

E

E

A

Y

H

P

P

E

E

V

L

R

K

P

N

L
x
V

F
|
F

N
|
N

Q

M

A
|
A

H
|
H

Q

Q

A

E

S

Q

G

G

D

Q

Q

Q

P

Q

R

Q

A

A

L

L

A

A

N

R

G

A

V

V

L

Y

M

A

Y

Y

A

A

R

E

H

N

I

I

D

E

Q

D

L

P

D

N

Q

S

L

L

G

M

D

A

L

V

V

L

A

K

K

N

I
|
I

I

A

N

N

K

K

H
|
H

V

A

A

S

L
|
L

Q

G

I
x
V

L

K

P

P

E

E

H
x
Q

Y
|
Y

P

P

I

I

V
|
V

G

G

S

E

C

H

L

L

L

L

R

A

A

A

I

I

S

K

E

E

V

V

L

L

G

G

E

N

E

A

I

-

A

A

T

T

P

D

E

D

V

I

M

I

S

S

A

A

W

W

G

A

A

Q

A

A

Y
|
Y

G

G

Q

N

L
|
L

A
|
A

E

D

I

V

L
|
L

I

M

G

G

A

M

E

E

A

S

S

E

I

L

Y

Y

D

E

Q

R

K

S

A

A

Q

E

A

Q

P

P

G

G

G

G

W

W

R

K

G

G

A

W

R

R

E

T

F

F

I

V

V

I

V

R

A

E

K

K

V

R

E

P

E

E

S

S

A

D

E

V

I

I

I

T

S

S

F

F

Y

I

F

L

E

E

P

P

A

A

D

D

K

G

G

G

P

P

I

V

L

V

A

N

A

F

E

E

P

P

G

G

Q

Q

Y
|
Y

I

T

G

S

M

V

K

A

L

I

I

D

L

V

D

P

-

A

-

L

G

G

E

L

E

Q

I

Q

R

I

R
|
R

N
x
Q

Y
|
Y

S
|
S

L

L

S

S

A

D

L

M

A

P

N

N

K

G

G

R

Q

T

Y

Y

R

R

I

I

S
|
S

V
|
V

K
|
K

R

R

E
|
E

T
x
G

G

G

-

G

-

P

-
x
Q

-
x
P

-

P

G
|
G

R
x
Y

A
x
V

S
|
S

N

N

H

L

L

L

H

H

D

D

Q

H

L

V

H

N

V

V

G

G

A

D

S

Q

I

V

L

K

L

L

F

A

P

A

P

P

A

Y

G

G

D

S

F

F

T

H

L

I

T

D

A

V

S

D

D

A

K

K

-

T

P

P

L

I

V

V

L

L

I

I

S

S

G

G

G

G

V

V

G

G

I

L

T

T

P

P

T

M

L

V

A

S

M

M

L

L

E

K

A

V

A

A

L

L

-

Q

A

A

T

P

E

P

R

R

P

Q

V

V

H

V

F

F

I

V

H

H

C

G

A

A

R

R

N

N

G

S

S

A

V

V

H

H

A

A

F

M

R

R

D

D

W

R

I

L

D

R

G

E

L

A

A

A

E

K

R

T

H

Y

P

E

Q

N

L

L

K

D

R

L

F

F

Y

V

C

F

Y

Y

A

D

E

Q

-

P

-

L

-

P

D

E

D

D

G

V

I

Q

S

G

P

R

A

D

A

Y

D

D

K

Y

V

P

G

G

L

L

L

V

S

D

Q

V

E

K

Q

Q

L

I

G

E

E

K

-

S

-

I

W

L

L

L

P

P

E

-

Q

-

R

-

D

D

V

A

D

D

A

Y

Y

Y

F

I

L

C

G

G

P

P

K

I

G

P

F

F

M

M

A

R

A

M

I

Q

K

H

R

D

H

A

L

L

K

K

A

N

L

L

G

G

V

I

P

H

E

E

K

A

Q

R

S

I

R

H

Y

Y

E
|
E

F
x
V

F

F

G
|
G

P
|
P

binding flavin-adenine dinucleotide: N44 (= N44), A46 (= A46), H47 (= H47), Y190 (= Y191), R206 (= R205), Q207 (≠ N206), Y208 (= Y207), S209 (= S208), S222 (= S221), V223 (= V222), K224 (= K223), E226 (= E225), G227 (≠ T226), Q231 (vs. gap), P232 (vs. gap), G234 (= G228), Y235 (≠ R229), V236 (≠ A230), S237 (= S231), E394 (= E384), V395 (≠ F385), G397 (= G387), P398 (= P388)
binding protoporphyrin ix containing fe: V42 (≠ L42), F43 (= F43), N44 (= N44), I81 (= I81), H85 (= H85), L88 (= L88), V90 (≠ I90), Q94 (≠ H94), Y95 (= Y95), V98 (= V98), Y126 (= Y127), L129 (= L130), A130 (= A131), L133 (= L134)

1gvhA The x-ray structure of ferric escherichia coli flavohemoglobin reveals an unespected geometry of the distal heme pocket (see paper)
42% identity, 100% coverage: 1:392/393 of query aligns to 1:396/396 of 1gvhA

query
sites
1gvhA

M

M

L

L

S

D

V

A

Q

Q

D

T

R

I

A

A

I

T

V

V

K

K

S

A

T

T

V

I

P

P

L

L

L

L

E

V

S

E

G

T

G

G

E

P

A

K

L

L

I

T

T

A

H

H

F

F

Y

Y

R

D

M

R

M

M

L

F

S

T

E

H

Y

N

P

P

E

E

V

L

R

K

P

E

L
x
I

F
|
F

N
|
N

Q

M

A
x
S

H

N

Q
|
Q

A

R

S
x
N

G

G

D

D

Q
|
Q

P

R

R

E

A
|
A

L
|
L

A

F

N

N

G
x
A

V
x
I

L

A

M

A

Y

Y

A

A

R

S

H

N

I

I

D

E

Q

N

L

L

D

P

Q

A

L

L

G

L

D

P

L

A

V

V

A

E

K

K

I
|
I

I

A

N

Q

K
|
K

H
|
H

V

T

A

S

L
x
F

Q

Q

I
|
I

L

K

P

P

E

E

H
x
Q

Y
|
Y

P

N

I

I

V
|
V

G

G

S

E

C

H

L

L

L

L

R

A

A

T

I

L

S

D

E

E

V

M

L

F

G

S

E

-

E

-

I

-

A

P

T

G

P

Q

E

E

V

V

M

L

S

D

A

A

W

W

G

G

A

K

A

A

Y
|
Y

G

G

Q

V

L
|
L

A
|
A

E

N

I

V

L
x
F

I

I

G

N

A

R

E

E

A

A

S

E

I

I

Y

Y

D

N

Q

E

K

N

A

A

Q

S

A

K

P

A

G

G

G

G

W

W

R

E

G

G

A

T

R

R

E

D

F

F

I

R

V

I

V

V

A

A

K

K

V

T

E

P

E

R

S

S

A

A

E

L

I

I

I

T

S

S

F

F

Y

E

F

L

E

E

P

P

A

V

D

D

K

G

G

G

P

A

I

V

L

A

A

E

A

Y

E

R

P

P

G

G

Q

Q

Y
|
Y

I

L

G

G

M

V

K

W

L

L

I

K

L

P

D

E

G

G

-

F

-

P

-

H

E

Q

E

E

I

I

R
|
R

R
x
Q

N

-

Y
|
Y

S
|
S

L

L

S

T

A

R

L

K

A

P

N

D

K

G

G

K

Q

G

Y

Y

R

R

I

I

S
x
A

V
|
V

K
|
K

R

R

E
|
E

T
x
E

G
|
G

G
|
G

R
x
Q

A
x
V

S
|
S

N

N

H

W

L

L

H

H

D

N

Q

H

L

A

H

N

V

V

G

G

A

D

S

V

I

V

L

K

L

L

F

V

P

A

P

P

A

A

G

G

D

D

F

F

T

F

L

M

-

A

T

V

A

A

S

D

D

D

K

T

P

P

L

V

V

T

L

L

I

I

S

S

G

A

G

G

V
|
V

G

G

I

Q

T
|
T

P

P

T

M

L

L

A

A

M

M

L

L

E

D

-

T

-

L

A

A

A

K

L

A

A

G

T

H

E

T

R

A

P

Q

V

V

H

N

F

W

I

F

H

H

C

A

A

A

R

E

N

N

G

G

S

D

V

V

H

H

A

A

F

F

R

A

D

D

W

E

I

V

D

K

G

E

L

L

A

G

E

Q

R

S

H

L

P

P

Q

R

L

F

K

T

R

A

F

H

Y

T

C

W

Y

Y

-

R

-

Q

-

P

A

S

E

E

D

A

D

D

G

R

I

A

S

K

P

G

A

Q

A

F

D

D

K

S

V

E

G

G

L

L

L

M

S

D

Q

L

E

S

Q

K

L

L

G

-

E

E

W

G

L

A

P

F

E

S

Q

D

R

P

D

T

V

M

D

Q

A

F

Y

Y

F

L

L

C

G

G

P

P

K

V

G

G

F

F

M

M

A

Q

A

F

I

T

K

A

R

K

H

Q

L

L

K

V

A

D

L

L

G

G

V

V

P

K

E

Q

K

E

Q

N

S

I

R

H

Y

Y

E
|
E

F
x
C

F
|
F

G

G

P
|
P

A
x
H

A

K

A

V

L

L

binding flavin-adenine dinucleotide: N44 (= N44), S46 (≠ A46), Q48 (= Q48), N50 (≠ S50), Y188 (= Y191), R204 (= R204), Q205 (≠ R205), Y206 (= Y207), S207 (= S208), A220 (≠ S221), V221 (= V222), K222 (= K223), E224 (= E225), E225 (≠ T226), G226 (= G227), G227 (= G228), Q228 (≠ R229), V229 (≠ A230), S230 (= S231), V269 (= V269), T272 (= T272), E388 (= E384), C389 (≠ F385), F390 (= F386)
binding protoporphyrin ix containing fe: I42 (≠ L42), F43 (= F43), N44 (= N44), Q53 (= Q53), A56 (= A56), L57 (= L57), A60 (≠ G60), I61 (≠ V61), I81 (= I81), K84 (= K84), H85 (= H85), F88 (≠ L88), I90 (= I90), Q94 (≠ H94), Y95 (= Y95), V98 (= V98), Y124 (= Y127), L127 (= L130), A128 (= A131), F131 (≠ L134), P392 (= P388), H393 (≠ A389)

P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 100% coverage: 1:392/393 of query aligns to 1:396/396 of P24232

query
sites
P24232

M

M

L

L

S

D

V

A

Q

Q

D

T

R

I

A

A

I

T

V

V

K

K

S

A

T

T

V

I

P

P

L

L

L

L

E

V

S

E

G

T

G

G

E

P

A

K

L

L

I

T

T

A

H

H

F

F

Y
|
Y

R

D

M

R

M

M

L

F

S

T

E

H

Y

N

P

P

E

E

V

L

R

K

P

E

L

I

F

F

N

N

Q

M

A

S

H

N

Q

Q

A

R

S

N

G

G

D

D

Q

Q

P

R

R

E

A

A

L

L

A

F

N

N

G

A

V

I

L

A

M

A

Y

Y

A

A

R

S

H

N

I

I

D

E

Q

N

L

L

D

P

Q

A

L

L

G

L

D

P

L

A

V

V

A

E

K

K

I

I

I

A

N

Q

K

K

H

H

V

T

A

S

L

F

Q

Q

I

I

L

K

P

P

E

E

H

Q

Y
|
Y

P

N

I

I

V

V

G

G

S

E

C

H

L

L

L

L

R

A

A

T

I

L

S

D

E

E

V

M

L

F

G

S

E

-

E

-

I

-

A

P

T

G

P

Q

E

E

V

V

M

L

S

D

A

A

W

W

G

G

A

K

A

A

Y

Y

G

G

Q

V

L

L

A

A

E

N

I

V

L

F

I

I

G

N

A

R

E
|
E

A

A

S

E

I

I

Y

Y

D

N

Q

E

K

N

A

A

Q

S

A

K

P

A

G

G

G

G

W

W

R

E

G

G

A

T

R

R

E

D

F

F

I

R

V

I

V

V

A

A

K

K

V

T

E

P

E

R

S

S

A

A

E

L

I

I

I

T

S

S

F

F

Y

E

F

L

E

E

P

P

A

V

D

D

K

G

G

G

P

A

I

V

L

A

A

E

A

Y

E

R

P

P

G

G

Q

Q

Y

Y

I

L

G

G

M

V

K

W

L

L

I

K

L

P

D

E

G

G

-

F

-

P

-

H

E

Q

E

E

I

I

R

R

R

Q

N

-

Y

Y

S

S

L

L

S

T

A

R

L

K

A

P

N

D

K

G

G

K

Q

G

Y

Y

R

R

I

I

S

A

V

V

K

K

R

R

E

E

T

E

G

G

G

G

R

Q

A

V

S

S

N

N

H

W

L

L

H

H

D

N

Q

H

L

A

H

N

V

V

G

G

A

D

S

V

I

V

L

K

L

L

F

V

P

A

P

P

A

A

G

G

D

D

F

F

T

F

L

M

-

A

T

V

A

A

S

D

D

D

K

T

P

P

L

V

V

T

L

L

I

I

S

S

G

A

G

G

V

V

G

G

I

Q

T

T

P

P

T

M

L

L

A

A

M

M

L

L

E

D

-

T

-

L

A

A

A

K

L

A

A

G

T

H

E

T

R

A

P

Q

V

V

H

N

F

W

I

F

H

H

C

A

A

A

R

E

N

N

G

G

S

D

V

V

H

H

A

A

F

F

R

A

D

D

W

E

I

V

D

K

G

E

L

L

A

G

E

Q

R

S

H

L

P

P

Q

R

L

F

K

T

R

A

F

H

Y

T

C

W

Y

Y

-

R

-

Q

-

P

A

S

E

E

D

A

D

D

G

R

I

A

S

K

P

G

A

Q

A

F

D

D

K

S

V

E

G

G

L

L

L

M

S

D

Q

L

E

S

Q

K

L

L

G

-

E

E

W

G

L

A

P

F

E

S

Q

D

R

P

D

T

V

M

D

Q

A

F

Y

Y

F

L

L

C

G

G

P

P

K

V

G

G

F

F

M

M

A

Q

A

F

I

T

K

A

R

K

H

Q

L

L

K

V

A

D

L

L

G

G

V

V

P

K

E

Q

K

E

Q

N

S

I

R

H

Y

Y

E

E

F

C

F

F

G

G

P

P

A

H

A

K

A

V

L

L

Y29 (= Y29) mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; mutation to F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
Y95 (= Y95) active site, Charge relay system
E135 (= E138) active site, Charge relay system

4g1bA X-ray structure of yeast flavohemoglobin in complex with econazole (see paper)
38% identity, 99% coverage: 1:388/393 of query aligns to 1:392/398 of 4g1bA

query
sites
4g1bA

M

M

L

L

S

A

V

E

Q

K

D

T

R

R

A

S

I

I

V

I

K

K

S

A

T

T

V

V

P

P

L

V

L

L

E

E

S

Q

G

Q

G

G

E

T

A

V

L

I

I
x
T

T

R

H

T

F
|
F

Y
|
Y

R

K

M

N

M

M

L

L

S

T

E

E

Y

H

P

T

E

E

V

L

R

L

P

N

L
x
I

F
|
F

N
|
N

Q

R

A
x
T

H
x
N

Q

Q

A
x
K

S
x
V

G

G

D

A

Q
|
Q

P

P

R

N

A
|
A

L
|
L

A

A

N

T

G
x
T

V
|
V

L

L

M

A

Y

A

A

A

R

K

H

N

I

I

D

D

Q

D

L

L

D

S

Q

V

L

L

G

M

D

D

L

H

V

V

A

K

K
x
Q

I
|
I

I

G

N

H

K
|
K

H
|
H

V

R

A

A

L
|
L

Q

Q

I
|
I

L

K

P

P

E

E

H
|
H

Y
|
Y

P

P

I

I

V
|
V

G

G

S

E

C

Y

L
|
L

L

L

R

K

A

A

I

I

S

K

E

E

V

V

L

L

G

G

E

D

E

A

I

-

A

A

T

T

P

P

E

E

V

I

M

I

S

N

A

A

W

W

G

G

A

E

A

A

Y
|
Y

G

Q

Q

A

L

I

A
|
A

E

D

I

I

L
x
F

I

I

G

T

A

V

E

E

A

K

S

K

I

M

Y

Y

D

E

Q

E

K

-

A

-

Q

-

A

-

P

-

G

A

G

L

W

W

R

P

G

G

A

W

R

K

E

P

F

F

I

E

V

I

V

T

A

A

K

K

V

E

E

Y

E

V

S

A

A

S

E

D

I

I

I

V

S

E

F

F

Y

T

F

V

E

K

P

P

A

K

D

F

K

G

G

S

P

G

I

I

-

E

-

L

-

E

-

S

L

L

A

P

A

I

E

T

P

P

G

G

Q

Q

Y
|
Y

I

I

G

T

M

V

K

N

L

T

I

H

L

P

D

I

G

R

E

Q

E

E

I

N

R

Q

-

Y

-

D

-

A

-

L

R
|
R

N
x
H

Y
|
Y

S
|
S

L

L

S

C

A

S

L

A

A

S

N

T

K

K

G

N

Q

G

Y

L

R

R

I

F

S
x
A

V
|
V

K
|
K

R

M

E
|
E

T

A

-

A

-

R

-

E

-

N

-
x
F

-
x
P

G

A

G
|
G

R
x
L

A
x
V

S
|
S

N

E

H

Y

L

L

H

H

D

K

Q

D

L

A

H

K

V

V

G

G

A

D

S

E

I

I

L

K

L

L

F

S

P

A

P

P

A

A

G

G

D

D

F

F

T

A

L

I

T

N

A

K

-

E

-

L

-

I

-

H

-

Q

S

N

D

E

K

V

P

P

L

L

V

V

L

L

I

L

S

S

G

S

G

G

V
|
V

G

G

I

V

T
|
T

P

P

T

L

L

L

A

A

M

M

L

L

E

E

A

E

A

Q

L

V

A

K

T

C

-

N

-

P

E

N

R

R

P

P

V

I

H

Y

F

W

I

I

H

Q

C

S

A

S

R

Y

N

D

G

E

S

K

V

T

H

Q

A

A

F

F

R

K

D

K

W

H

I

V

D

D

G

E

L

L

A

L

E

A

R

E

H

-

P

-

Q

-

L

-

K

-

R

-

F

-

Y

-

C

C

Y

-

A

A

E

N

D

V

D

D

G

K

I

I

S

I

P

V

A

H

A

T

D

D

K

T

V

E

G

P

L

L

L

I

S

N

Q

A

E

A

Q

F

L

L

G

K

E

E

W

K

L

S

P

P

E

A

Q

H

R

A

D

D

V

V

D

-

A

-

Y

Y

F

T

L

C

G

G

P

S

K

L

G

A

F

F

M

M

A

Q

A

A

I

M

K

I

R

G

H

H

L

L

K

K

A

E

L

L

G

E

V

H

P

R

E

D

K

D

Q

M

S

I

R

H

Y

Y

E
|
E

F
x
P

F
|
F

G
|
G

P
|
P

binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: T25 (≠ I25), F28 (= F28), Y29 (= Y29), F43 (= F43), Q53 (= Q53), A56 (= A56), L57 (= L57), T60 (≠ G60), V61 (= V61), V98 (= V98), L102 (= L102), Y126 (= Y127)
binding flavin-adenine dinucleotide: N44 (= N44), T46 (≠ A46), K49 (≠ A49), V50 (≠ S50), K84 (= K84), Y189 (= Y191), R207 (= R205), H208 (≠ N206), Y209 (= Y207), S210 (= S208), A223 (≠ S221), V224 (= V222), K225 (= K223), E227 (= E225), F233 (vs. gap), P234 (vs. gap), G236 (= G228), L237 (≠ R229), V238 (≠ A230), S239 (= S231), V282 (= V269), T285 (= T272), E388 (= E384), P389 (≠ F385), F390 (= F386), G391 (= G387)
binding protoporphyrin ix containing fe: I42 (≠ L42), F43 (= F43), N44 (= N44), N47 (≠ H47), T60 (≠ G60), Q80 (≠ K80), I81 (= I81), K84 (= K84), H85 (= H85), L88 (= L88), I90 (= I90), H94 (= H94), Y95 (= Y95), V98 (= V98), Y126 (= Y127), A130 (= A131), F133 (≠ L134), P392 (= P388)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 393, 394

4g1vA X-ray structure of yeast flavohemoglobin (see paper)
38% identity, 98% coverage: 2:388/393 of query aligns to 1:391/398 of 4g1vA

query
sites
4g1vA

L

L

S

A

V

E

Q

K

D

T

R

R

A

S

I

I

V

I

K

K

S

A

T

T

V

V

P

P

L

V

L

L

E

E

S

Q

G

Q

G

G

E

T

A

V

L

I

I

T

T

R

H

T

F
|
F

Y
|
Y

R

K

M

N

M

M

L

L

S

T

E

E

Y

H

P

T

E

E

V

L

R

L

P

N

L
x
I

F
|
F

N
|
N

Q

R

A
x
T

H
x
N

Q

Q

A

K

S
x
V

G

G

D

A

Q
|
Q

P

P

R

N

A
|
A

L
|
L

A

A

N

T

G
x
T

V

V

L

L

M

A

Y

A

A

A

R

K

H

N

I

I

D

D

Q

D

L

L

D

S

Q

V

L

L

G

M

D

D

L

H

V

V

A

K

K
x
Q

I
|
I

I

G

N

H

K
|
K

H
|
H

V

R

A

A

L
|
L

Q

Q

I
|
I

L

K

P

P

E

E

H
|
H

Y
|
Y

P

P

I

I

V
|
V

G

G

S

E

C

Y

L

L

L

L

R

K

A

A

I

I

S

K

E

E

V

V

L

L

G

G

E

D

E

A

I

-

A

A

T

T

P

P

E

E

V

I

M

I

S

N

A

A

W

W

G

G

A

E

A

A

Y
|
Y

G

Q

Q

A

L
x
I

A
|
A

E

D

I

I

L
x
F

I

I

G

T

A

V

E

E

A

K

S

K

I

M

Y

Y

D

E

Q

E

K

-

A

-

Q

-

A

-

P

-

G

A

G

L

W

W

R

P

G

G

A

W

R

K

E

P

F

F

I

E

V

I

V

T

A

A

K

K

V

E

E

Y

E

V

S

A

A

S

E

D

I

I

I

V

S

E

F

F

Y

T

F

V

E

K

P

P

A

K

D

F

K

G

G

S

P

G

I

I

-

E

-

L

-

E

-

S

L

L

A

P

A

I

E

T

P

P

G

G

Q

Q

Y
|
Y

I

I

G

T

M

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N

L

T

I

H

L

P

D

I

G

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Q

E

E

I

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-

Y

-

D

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-

L

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R

N
x
H

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Y

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S

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C

A

S

L

A

A

S

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T

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x
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V

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K

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E

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-

A

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x
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N

-
x
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x
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S

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G

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A

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-

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-

L

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C

Y

-

A

A

E

N

D

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A

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K

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A

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K

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P

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R

A

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D

V

V

D

-

A

-

Y

Y

F

T

L

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G

A

F

F

M

M

A

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A

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L

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K

A

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Y

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E
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E

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x
P

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P

binding flavin-adenine dinucleotide: N43 (= N44), T45 (≠ A46), V49 (≠ S50), K83 (= K84), Y188 (= Y191), R206 (= R205), H207 (≠ N206), Y208 (= Y207), S209 (= S208), A222 (≠ S221), V223 (= V222), K224 (= K223), E226 (= E225), R229 (vs. gap), F232 (vs. gap), P233 (vs. gap), G235 (= G228), L236 (≠ R229), V237 (≠ A230), S238 (= S231), V281 (= V269), T284 (= T272), E387 (= E384), P388 (≠ F385), F389 (= F386), G390 (= G387)
binding protoporphyrin ix containing fe: I41 (≠ L42), F42 (= F43), N43 (= N44), N46 (≠ H47), Q52 (= Q53), A55 (= A56), L56 (= L57), T59 (≠ G60), Q79 (≠ K80), I80 (= I81), K83 (= K84), H84 (= H85), L87 (= L88), I89 (= I90), H93 (= H94), Y94 (= Y95), V97 (= V98), Y125 (= Y127), I128 (≠ L130), A129 (= A131), F132 (≠ L134), P391 (= P388)
binding nitrite ion: F27 (= F28), Y28 (= Y29), F42 (= F43), L56 (= L57)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 392, 393

Q03331 Flavohemoprotein; Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Candida norvegensis (Yeast) (Candida mycoderma) (see paper)
30% identity, 99% coverage: 2:389/393 of query aligns to 13:386/390 of Q03331

query
sites
Q03331

L

L

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A

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G

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T

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylserine

6o0aA Crystal structure of flavohemoglobin from malassezia yamatoensis with bound fad and heme determined by iron sad phasing (see paper)
31% identity, 98% coverage: 2:387/393 of query aligns to 3:383/383 of 6o0aA

query
sites
6o0aA

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H
&n