SitesBLAST
Comparing Pf6N2E2_1323 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1323 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
44% identity, 97% coverage: 7:254/255 of query aligns to 7:253/259 of 6ci9D
- active site: G20 (= G20), S145 (= S145), Y159 (= Y159)
- binding 1-aminopropan-2-one: F97 (= F97), S145 (= S145), T147 (= T147), W156 (≠ L156), Y159 (= Y159), G190 (= G190)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G16), S18 (≠ A18), G20 (= G20), I21 (= I21), G40 (≠ D40), R41 (≠ L41), N42 (≠ G42), D66 (= D66), V67 (≠ L67), N93 (= N93), G95 (= G95), T143 (= T143), S145 (= S145), Y159 (= Y159), K163 (= K163), P189 (= P189), N191 (≠ M191), I192 (= I192), T194 (= T194), G196 (≠ A196), L197 (≠ M197)
3pk0B Crystal structure of short-chain dehydrogenase/reductase sdr from mycobacterium smegmatis (see paper)
40% identity, 98% coverage: 5:254/255 of query aligns to 6:255/262 of 3pk0B
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
37% identity, 94% coverage: 10:249/255 of query aligns to 3:252/256 of Q48436
- 6:33 (vs. 13:40, 54% identical) binding NAD(+)
- D59 (= D66) binding NAD(+)
- K156 (= K163) binding NAD(+)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
37% identity, 94% coverage: 10:249/255 of query aligns to 3:252/256 of 1gegE
- active site: G13 (= G20), S139 (≠ V146), Y152 (= Y159), K156 (= K163), V197 (= V205)
- binding alpha-D-glucopyranose: R63 (≠ A70), D64 (≠ T71), F67 (= F74), E123 (≠ P130)
- binding nicotinamide-adenine-dinucleotide: G9 (= G16), Q12 (= Q19), I14 (= I21), D33 (= D40), Y34 (≠ L41), V58 (= V65), D59 (= D66), V60 (≠ L67), N86 (= N93), A87 (= A94), I109 (≠ V116), S139 (≠ V146), Y152 (= Y159), K156 (= K163), P182 (= P189), V185 (≠ I192), T187 (= T194), M189 (≠ A196)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 94% coverage: 10:249/255 of query aligns to 8:241/244 of 4nbuB
- active site: G18 (= G20), N111 (= N117), S139 (= S145), Q149 (≠ L156), Y152 (= Y159), K156 (= K163)
- binding acetoacetyl-coenzyme a: D93 (≠ L99), K98 (≠ E104), S139 (= S145), N146 (≠ Y153), V147 (≠ P154), Q149 (≠ L156), Y152 (= Y159), F184 (≠ M191), M189 (≠ A196), K200 (≠ D208)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G16), N17 (≠ Q19), G18 (= G20), I19 (= I21), D38 (= D40), F39 (≠ L41), V59 (= V65), D60 (= D66), V61 (≠ L67), N87 (= N93), A88 (= A94), G89 (= G95), I90 (≠ Y96), T137 (= T143), S139 (= S145), Y152 (= Y159), K156 (= K163), P182 (= P189), F184 (≠ M191), T185 (≠ I192), T187 (= T194), M189 (≠ A196)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
38% identity, 94% coverage: 10:249/255 of query aligns to 5:243/246 of 3osuA
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
38% identity, 94% coverage: 10:249/255 of query aligns to 6:250/256 of 7do7A
- active site: G16 (= G20), S146 (≠ V146), Y159 (= Y159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G16), R15 (≠ Q19), G16 (= G20), I17 (= I21), S37 (vs. gap), D66 (= D66), A67 (≠ L67), N93 (= N93), A94 (= A94), G95 (= G95), I96 (≠ Y96), V144 (≠ S144), S145 (= S145), S146 (≠ V146), Y159 (= Y159), K163 (= K163), P189 (= P189), G190 (= G190), I192 (= I192), T194 (= T194), I196 (vs. gap)
- binding beta-L-rhamnopyranose: F99 (≠ L99), S146 (≠ V146), S148 (≠ G148), Q156 (≠ L156), Y159 (= Y159), N197 (≠ A196), D235 (≠ S234), M236 (≠ L235), R238 (≠ S237)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
38% identity, 94% coverage: 10:249/255 of query aligns to 6:250/256 of 7b81A
- active site: G16 (= G20), S146 (≠ V146), Y159 (= Y159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G16), S14 (≠ A18), R15 (≠ Q19), I17 (= I21), D66 (= D66), A67 (≠ L67), N93 (= N93), A94 (= A94), G95 (= G95), I96 (≠ Y96), T116 (≠ V116), V144 (≠ S144), S146 (≠ V146), Y159 (= Y159), K163 (= K163), P189 (= P189), G190 (= G190), I192 (= I192), T194 (= T194), I196 (vs. gap)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
38% identity, 97% coverage: 5:251/255 of query aligns to 3:253/255 of 5itvA
- active site: G18 (= G20), S141 (= S145), Y154 (= Y159), K158 (= K163)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G16), S17 (≠ Q19), G18 (= G20), I19 (= I21), D38 (= D40), I39 (≠ L41), T61 (≠ V65), I63 (≠ L67), N89 (= N93), G91 (= G95), T139 (= T143), S141 (= S145), Y154 (= Y159), K158 (= K163), P184 (= P189), G185 (= G190), I186 (≠ M191), I187 (= I192)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
38% identity, 94% coverage: 10:249/255 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G20), S138 (= S145), Q148 (≠ L156), Y151 (= Y159), K155 (= K163)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), S10 (≠ A18), R11 (≠ Q19), I13 (= I21), N31 (≠ A39), Y32 (≠ D40), A33 (≠ L41), G34 (= G42), S35 (vs. gap), A58 (≠ V65), N59 (≠ D66), V60 (≠ L67), N86 (= N93), A87 (= A94), T109 (≠ V116), S138 (= S145), Y151 (= Y159), K155 (= K163), P181 (= P189), G182 (= G190)
7do6A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NADP bound-form) (see paper)
38% identity, 94% coverage: 10:249/255 of query aligns to 6:241/247 of 7do6A
- active site: G16 (= G20), S146 (≠ V146), Y159 (= Y159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G16), S14 (≠ A18), R15 (≠ Q19), G16 (= G20), I17 (= I21), H36 (vs. gap), S37 (vs. gap), G42 (= G42), D66 (= D66), A67 (≠ L67), N93 (= N93), A94 (= A94), G95 (= G95), I96 (≠ Y96), T116 (≠ V116), S146 (≠ V146), Y159 (= Y159), K163 (= K163), I192 (= I192)
7krmC Putative fabg bound to nadh from acinetobacter baumannii
39% identity, 95% coverage: 7:249/255 of query aligns to 3:240/244 of 7krmC
- active site: G18 (= G20), S140 (= S145), Y155 (= Y159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G16), S15 (vs. gap), G18 (= G20), I19 (= I21), D38 (= D40), L39 (= L41), A60 (≠ V65), N61 (≠ D66), V62 (≠ L67), N88 (= N93), V111 (= V116), S140 (= S145), Y155 (= Y159), K159 (= K163), I188 (= I192), T190 (= T194)
5fffA Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and piperonal (see paper)
35% identity, 99% coverage: 1:252/255 of query aligns to 3:255/257 of 5fffA
- active site: K206 (≠ D202)
- binding 1,3-benzodioxole-5-carbaldehyde: Y100 (≠ F97), H158 (≠ L156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G16), T20 (≠ A18), G22 (= G20), I23 (= I21), R43 (≠ L41), C67 (≠ V65), D68 (= D66), V69 (≠ L67), N96 (= N93), I146 (≠ T143), Y161 (= Y159), K165 (= K163), P191 (= P189), A193 (≠ M191), I194 (= I192), T196 (= T194), G198 (≠ A196), T199 (≠ M197)
5ff9B Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and tyramine (see paper)
35% identity, 99% coverage: 1:252/255 of query aligns to 3:255/257 of 5ff9B
- active site: K206 (≠ D202)
- binding 4-(2-aminoethyl)phenol: Y100 (≠ F97), I155 (≠ Y153), H158 (≠ L156)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G16), T20 (≠ A18), K21 (≠ Q19), I23 (= I21), S42 (≠ D40), R43 (≠ L41), C67 (≠ V65), D68 (= D66), V69 (≠ L67), N96 (= N93), I146 (≠ T143), S148 (= S145), Y161 (= Y159), K165 (= K163), P191 (= P189), A193 (≠ M191), I194 (= I192), T196 (= T194), G198 (≠ A196), T199 (≠ M197)
A0A1A9TAK5 Noroxomaritidine/norcraugsodine reductase; NorRed; EC 1.1.1.- from Narcissus pseudonarcissus (Daffodil) (see paper)
35% identity, 99% coverage: 1:252/255 of query aligns to 3:255/257 of A0A1A9TAK5
- TKGI 20:23 (≠ AQGI 18:21) binding NADP(+)
- SR 42:43 (≠ DL 40:41) binding NADP(+)
- DV 68:69 (≠ DL 66:67) binding NADP(+)
- NAG 96:98 (= NAG 93:95) binding NADP(+)
- Y100 (≠ F97) binding substrate
- C149 (≠ V146) binding substrate
- Y161 (= Y159) binding NADP(+)
- K165 (= K163) binding NADP(+)
- IRTPGT 194:199 (≠ IATPAM 192:197) binding NADP(+)
6y4dA Crystal structure of a short-chain dehydrogenase/reductase (sdr) from zephyranthes treatiae in complex with NADP+ (see paper)
36% identity, 99% coverage: 1:252/255 of query aligns to 3:255/257 of 6y4dA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G16), T20 (≠ A18), K21 (≠ Q19), I23 (= I21), R43 (= R45), C67 (≠ A72), D68 (≠ V73), I69 (≠ F74), N96 (vs. gap), G98 (= G95), V146 (≠ T143), S147 (= S144), S148 (= S145), Y161 (= Y159), K165 (= K163), G192 (= G190), I194 (= I192), T196 (= T194), S198 (≠ A196)
Q9ZW19 Tropinone reductase homolog At2g29360; EC 1.1.1.- from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 96% coverage: 9:252/255 of query aligns to 18:262/271 of Q9ZW19
- S209 (≠ N199) mutation to Y: Loss of activity with quinuclidinone and decreased activity with cyclohexanones.
A7DY56 Tropinone reductase; EC 1.1.1.206; EC 1.1.1.236 from Cochlearia officinalis (Common scurvygrass) (see paper)
35% identity, 95% coverage: 9:249/255 of query aligns to 18:259/273 of A7DY56
- Y209 (vs. gap) mutation to S: Loss of tropinone or nortropinone reduction, but faster reduction of cyclohexanones.
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
38% identity, 93% coverage: 12:249/255 of query aligns to 4:241/244 of 1edoA
- active site: G12 (= G20), S138 (= S145), Y151 (= Y159), K155 (= K163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G16), S10 (≠ A18), R11 (≠ Q19), I13 (= I21), N31 (≠ A39), Y32 (≠ D40), A33 (≠ L41), R34 (≠ G42), S35 (≠ L43), D59 (= D66), V60 (≠ L67), N86 (= N93), A87 (= A94), S138 (= S145), Y151 (= Y159), K155 (= K163), P181 (= P189), G182 (= G190), I184 (= I192), S186 (≠ T194), M188 (≠ A196)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
37% identity, 96% coverage: 7:252/255 of query aligns to 3:239/239 of 4nbtA
- active site: G16 (= G20), S132 (= S145), Y145 (= Y159), K149 (= K163)
- binding nicotinamide-adenine-dinucleotide: G12 (= G16), K15 (≠ Q19), G16 (= G20), L17 (≠ I21), D36 (= D40), L37 (= L41), L52 (≠ V65), N53 (≠ D66), V54 (≠ L67), N80 (= N93), A81 (= A94), G82 (= G95), I130 (≠ T143), S132 (= S145), Y145 (= Y159), K149 (= K163), P177 (= P189), G178 (= G190), I180 (= I192), T182 (= T194)
Query Sequence
>Pf6N2E2_1323 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1323
MSEDLDFSGQTVLVTGGAQGIGRAIVEAFALRGARVVIADLGLARAEAVADELTAEGCQV
QAVGVDLADATAVFGMMAELEQRLGRLDILIHNAGYFPLTPFAEITPSMLDRTLAVNLSA
LFWLTQAALPMFRRQGQGCVLVTSSVTGPRVAYPGLSHYAASKAGVNGFIRNAALELAAE
NVRVNGVEPGMIATPAMANLGDDEVNQDIARRVPLGRLGQPSDIAGAMLYLASSLASYVT
GQTLVVDGGSTLPEV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory