SitesBLAST
Comparing Pf6N2E2_1623 Siderophore biosynthesis diaminobutyrate--2-oxoglutarate aminotransferase (EC 2.6.1.76) to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
38% identity, 86% coverage: 40:450/476 of query aligns to 9:404/422 of 6rl5G
- active site: S16 (≠ P47), F137 (≠ Y168), D237 (= D276), K266 (= K305)
- binding pyridoxal-5'-phosphate: T109 (= T140), G110 (= G141), T111 (≠ A142), F137 (≠ Y168), H138 (= H169), G139 (= G170), E204 (= E243), D237 (= D276), I239 (≠ V278), Q240 (= Q279), K266 (= K305), G294 (= G332), T295 (= T333), F296 (= F334)
O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 98% coverage: 10:474/476 of query aligns to 7:474/474 of O58478
- D251 (≠ E248) mutation to A: Loss of activity.
- K308 (= K305) mutation to A: Loss of activity.
7vo1A Structure of aminotransferase-substrate complex (see paper)
33% identity, 90% coverage: 47:474/476 of query aligns to 31:452/452 of 7vo1A
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (≠ A77), S121 (≠ T140), G122 (= G141), T123 (≠ A142), F149 (≠ Y168), H150 (= H169), G151 (= G170), R152 (≠ M171), E229 (= E243), E234 (= E248), D262 (= D276), V264 (= V278), Q265 (= Q279), K291 (= K305), S317 (≠ A331), N318 (≠ G332), T319 (= T333), F320 (= F334), R417 (= R438)
7vntA Structure of aminotransferase-substrate complex (see paper)
33% identity, 90% coverage: 47:474/476 of query aligns to 31:452/452 of 7vntA
- binding L-ornithine: I61 (≠ A77), F149 (≠ Y168), R152 (≠ M171), E234 (= E248), K291 (= K305)
- binding pyridoxal-5'-phosphate: S121 (≠ T140), G122 (= G141), T123 (≠ A142), N126 (≠ I145), F149 (≠ Y168), H150 (= H169), G151 (= G170), E229 (= E243), D262 (= D276), V264 (= V278), Q265 (= Q279), K291 (= K305), N318 (≠ G332), T319 (= T333)
Sites not aligning to the query:
7vnoA Structure of aminotransferase (see paper)
33% identity, 90% coverage: 47:474/476 of query aligns to 31:452/452 of 7vnoA
- binding pyridoxal-5'-phosphate: S121 (≠ T140), G122 (= G141), T123 (≠ A142), N126 (≠ I145), F149 (≠ Y168), H150 (= H169), G151 (= G170), E229 (= E243), D262 (= D276), V264 (= V278), Q265 (= Q279), K291 (= K305), N318 (≠ G332), T319 (= T333), F320 (= F334)
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 86% coverage: 52:462/476 of query aligns to 21:387/387 of 1wkhA
- active site: F132 (≠ Y168), E184 (= E243), D217 (= D276), Q220 (= Q279), K246 (= K305), T275 (= T333), R363 (= R438)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A77), S104 (≠ T140), G105 (= G141), T106 (≠ A142), N109 (≠ I145), F132 (≠ Y168), S133 (≠ H169), G134 (= G170), E184 (= E243), E189 (= E248), D217 (= D276), I219 (≠ V278), Q220 (= Q279), K246 (= K305), T274 (≠ G332), T275 (= T333), R363 (= R438)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 86% coverage: 52:462/476 of query aligns to 21:387/387 of 1wkgA
- active site: F132 (≠ Y168), E184 (= E243), D217 (= D276), Q220 (= Q279), K246 (= K305), T275 (= T333), R363 (= R438)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ A77), S104 (≠ T140), G105 (= G141), T106 (≠ A142), N109 (≠ I145), F132 (≠ Y168), S133 (≠ H169), G134 (= G170), R135 (≠ M171), E184 (= E243), E189 (= E248), D217 (= D276), I219 (≠ V278), Q220 (= Q279), K246 (= K305), G273 (≠ A331), T274 (≠ G332), T275 (= T333)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 86% coverage: 52:462/476 of query aligns to 21:387/387 of 1vefA
- active site: F132 (≠ Y168), D217 (= D276), K246 (= K305), T275 (= T333), R363 (= R438)
- binding pyridoxal-5'-phosphate: S104 (≠ T140), G105 (= G141), T106 (≠ A142), N109 (≠ I145), F132 (≠ Y168), S133 (≠ H169), G134 (= G170), E184 (= E243), D217 (= D276), I219 (≠ V278), Q220 (= Q279), K246 (= K305), T274 (≠ G332), T275 (= T333)
Sites not aligning to the query:
P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
36% identity, 86% coverage: 55:463/476 of query aligns to 27:421/421 of P50457
- K267 (= K305) mutation to A: No GABA-AT activity.
Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
36% identity, 86% coverage: 52:462/476 of query aligns to 29:395/395 of Q5SHH5
- GT 113:114 (≠ GA 141:142) binding
- K254 (= K305) modified: N6-(pyridoxal phosphate)lysine
- T283 (= T333) binding
P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see paper)
32% identity, 85% coverage: 64:469/476 of query aligns to 78:457/459 of P42588