SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_1742 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1742 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
50% identity, 96% coverage: 10:453/463 of query aligns to 1:443/451 of 6g4fA

query
sites
6g4fA

T

S

E

S

L

L

A

P

R

E

T

K

D

D

M

I

R

Q

F

Y

H

Q

L

L

H

H

S

P

Q
x
Y

T

T

N

N

L

A

R

R

A

L

H

H

Q

Q

Q

E

H

L

G

G

P

P

L

L

V

I

I

I

E

E

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R

G

G

S

E

G

G

I

I

Y

Y

V

V

T

Y

D

D

E

D

H

Q

G

G

R

K

D

G

Y

Y

I

I

E

E

G

A

M

M

S

A

G

G

L

L

W

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

N

N

P

Q

R

R

L

L

V

I

Q

K

A

A

A

A

Q

E

R

Q

Q

Q

M

F

A

N

V

T

L

L

P

P

Y

F

Y

Y

H

H

T

L

F

F

N

S

H

H

R

K

V

S

P

H

N

R

V

P

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S

A

I

Q

E

L

L

A

A

E

E

R

K

I

L

A

I

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E

L

M

V

A

P

P

F

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E

P

K

M

P

S

K

K

V

V

F

F

F

F

A

T

C

N

S
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S

G
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G

S
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S

E

E

A

A

N

N

D

D

S

T

M

V

V

V

K

K

L

M

A

V

W

W

A

Y

Y

L

H

N

R

N

A

A

R

L

G

G

N

K

D

P

G

A

K

K

R

K

K

K

I

F

I

I

A

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H

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Q

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K

N

G

G

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x
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H
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H

G

G

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I

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T

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V

M

A

G

S

A

A

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S

L

L

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T

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G

L

L

P

P

N

G

M

N

H

Q

A

R

A

G

F

F

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D

L

L

P

P

L

L

E

P

N

G

S

-

V

F

L

L

H

H

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Q

G

C

C

P

P

H

H

F

H

Y

Y

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R

H

F

G

A

I

L

E

A

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G

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E

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S

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E

A

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H

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F

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A

E

D

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R

L

L

L

A

R

V

D

E

L

L

E

E

Q

Q

R

K

I

I

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L

A

A

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E

G

G

A

P

D

E

T

T

I

I

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A

A

A

F

F

I

I

S

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E
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E

P

P

V

L

M

M

G

G

A

A

G

G

G

G

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V

I

I

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Y

Y

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E

G

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A

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C

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K

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Y

D

D

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I

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L

I

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D

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E

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x
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I
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I

C

C

G

G

F

F

G

G

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T

G

G

R

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W

M

F

F

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P

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D

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I

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M

A

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K

S

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L

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S

S

S

G

S

Y

Y

Q

Q

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P

I

I

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C

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L

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N

E

A

N

P

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Y

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I

I

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E

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A

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L

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F

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Y

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P

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A

A

A

T

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A

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A

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N

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V

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R

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A

A

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P

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T

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G

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V

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E

A

R

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A

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A

A

R

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E

E

Q

H

G

G

L

M

I

I

V

T

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R

N

A

M

M

G

G

D

D

S

A

I

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A

A

L

F

A

C

P

P

P

P

F

L

I

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I

V

S

N

A

E

E

Q

E

E

I

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G

E

M

L

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V

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R

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F

T

A

R

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L

D

D

active site: Y15 (≠ Q24), Y146 (≠ F155), D253 (= D263), K282 (= K292)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (= S121), G113 (= G122), S114 (= S123), Y146 (≠ F155), H147 (= H156), E220 (= E230), D253 (= D263), V255 (≠ I265), I256 (= I266), K282 (= K292)

6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
50% identity, 96% coverage: 10:453/463 of query aligns to 1:443/451 of 6g4eA

query
sites
6g4eA

T

S

E

S

L

L

A

P

R

E

T

K

D

D

M

I

R

Q

F

Y

H

Q

L

L

H

H

S

P

Q
x
Y

T

T

N

N

L

A

R

R

A

L

H

H

Q

Q

Q

E

H

L

G

G

P

P

L

L

V

I

I

I

E

E

R

R

G

G

S

E

G

G

I

I

Y

Y

V

V

T

Y

D

D

E

D

H

Q

G

G

R

K

D

G

Y

Y

I

I

E

E

G

A

M

M

S

A

G

G

L

L

W

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

N

N

P

Q

R

R

L

L

V

I

Q

K

A

A

A

A

Q

E

R

Q

Q

Q

M

F

A

N

V

T

L

L

P

P

Y

F

Y

Y

H

H

T

L

F

F

N

S

H

H

R

K

V

S

P

H

N

R

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P

V

S

A

I

Q

E

L

L

A

A

E

E

R

K

I

L

A

I

S

E

L

M

V

A

P

P

F

V

E

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K

M

P

S

K

K

V

V

F

F

F

F

A

T

C

N

S
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S

G
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G

S
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S

E

E

A

A

N

N

D

D

S

T

M

V

V

V

K

K

L

M

A

V

W

W

A

Y

Y

L

H

N

R

N

A

A

R

L

G

G

N

K

D

P

G

A

K

K

R

K

K

K

I

F

I

I

A

S

H

R

Q

V

K

N

G

G

F
x
Y

H
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H

G

G

S

I

T

T

V

V

M

A

G

S

A

A

S

S

L

L

S

T

G

G

L

L

P

P

N

G

M

N

H

Q

A

R

A

G

F

F

G

D

L

L

P

P

L

L

E

P

N

G

S

-

V

F

L

L

H

H

V

V

Q

G

C

C

P

P

H

H

F

H

Y

Y

R

R

H

F

G

A

I

L

E

A

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G

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E

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E

A

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H

F

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T

A

E

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L

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A

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D

E

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L

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Q

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K

I

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L

A

A

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E

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G

A

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D

E

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T

I

I

A

A

A

A

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I

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E
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E

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M

G

G

A

A

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Y

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K

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Y

D

D

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L

I

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A

D
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D

E

E

I
x
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I

C

C

G

G

F

F

G

G

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R

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T

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R

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M

F

F

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M

A

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K

S

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S

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Y

Q

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P

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C

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I

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E

A

N

P

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Y

F

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I

I

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A

E

D

Q

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S

Q

Q

Q

A

L

L

G

G

G

A

F

L

G

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H

H

G

G

F

F

T

T

Y

G

S

S

G

G

H

H

P

P

V

V

A

A

A

T

A

A

V

V

A

A

L

L

E

E

T

N

L

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L

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Y

I

Q

E

E

E

M

E

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S

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V

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A

E

A

E

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M

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G

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A

D

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P

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L

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V

G

G

E

E

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I

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R

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C

G

G

L

L

V

I

A

A

G

A

L

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E

E

L

L

V

V

V

G

D

D

K

R

H

V

T

S

R

K

A

A

P

P

F

Y

P

Q

R

A

E

L

I

G

A

T

I

L

G

G

V

R

Q

Y

V

M

E

A

R

G

A

R

A

A

R

Q

E

E

Q

H

G

G

L

M

I

I

V

T

R
|
R

N

A

M
|
M

G

G

D

D

S

A

I

V

A

A

L

F

A

C

P

P

P

P

F

L

I

I

I

V

S

N

A

E

E

Q

E

E

I

V

V

G

E

M

L

I

V

V

A

E

R

R

L

F

T

A

R

R

A

A

L

L

D

D

active site: Y15 (≠ Q24), Y146 (≠ F155), D253 (= D263), K282 (= K292)
binding 6-aminohexanoic acid: K282 (= K292), R412 (= R422), M414 (= M424)
binding pyridoxal-5'-phosphate: S112 (= S121), G113 (= G122), S114 (= S123), Y146 (≠ F155), H147 (= H156), E220 (= E230), D253 (= D263), V255 (≠ I265), I256 (= I266), K282 (= K292)

6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
50% identity, 96% coverage: 10:453/463 of query aligns to 1:443/453 of 6g4dB

query
sites
6g4dB

T

S

E

S

L

L

A

P

R

E

T

K

D

D

M

I

R

Q

F

Y

H

Q

L

L

H

H

S

P

Q
x
Y

T

T

N

N

L

A

R

R

A

L

H

H

Q

Q

Q

E

H

L

G

G

P

P

L

L

V

I

I

I

E

E

R

R

G

G

S

E

G

G

I

I

Y

Y

V

V

T

Y

D

D

E

D

H

Q

G

G

R

K

D

G

Y

Y

I

I

E

E

G

A

M

M

S

A

G

G

L

L

W

W

C

S

A

A

G

A

L

L

G

G

F

F

S

S

N

N

P

Q

R

R

L

L

V

I

Q

K

A

A

A

A

Q

E

R

Q

Q

Q

M

F

A

N

V

T

L

L

P

P

Y

F

Y

Y

H

H

T

L

F

F

N

S

H

H

R

K

V

S

P

H

N

R

V

P

V

S

A

I

Q

E

L

L

A

A

E

E

R

K

I

L

A

I

S

E

L

M

V

A

P

P

F

V

E

P

K

M

P

S

K

K

V

V

F

F

F

F

A

T

C

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

S

T

M

V

V

V

K

K

L

M

A

V

W

W

A

Y

Y

L

H

N

R

N

A

A

R

L

G

G

N

K

D

P

G

A

K

K

R

K

K

K

I

F

I

I

A

S

H

R

Q

V

K

N

G

G

F
x
Y

H
|
H

G

G

S

I

T

T

V

V

M

A

G

S

A

A

S

S

L

L

S

T

G

G

L

L

P

P

N

G

M

N

H

Q

A

R

A

G

F

F

G

D

L

L

P

P

L

L

E

P

N

G

S

-

V

F

L

L

H

H

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V

Q

G

C

C

P

P

H

H

F

H

Y

Y

R

R

H

F

G

A

I

L

E

A

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G

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E

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S

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E

A

E

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H

F

F

T

A

E

D

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R

L

L

L

A

R

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D

E

L

L

E

E

Q

Q

R

K

I

I

E

L

A

A

E

E

G

G

A

P

D

E

T

T

I

I

A

A

A

A

F

F

I

I

S

G

E
|
E

P

P

V

L

M

M

G

G

A

A

G

G

G

G

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I

I

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K

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Y

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D

I

I

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L

F

V

L

I

A

A

D
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D

E

E

I
x
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I
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I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

R

Q

W

M

F

F

G

G

H

S

Q

Q

T

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L

F

G

G

F

I

T

Q

P

P

D

D

M

I

M

M

A

V

C

L

A

S

K
|
K

S

Q

L

L

S

S

S

S

G

S

Y

Y

Q

Q

P

P

I

I

S

A

C

A

V

I

V

L

V

I

A

N

E

A

N

P

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Y

F

Q

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G

I

I

E

A

E

D

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S

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Q

Q

A

L

L

G

G

G

A

F

L

G

G

H

H

G

G

F

F

T

T

Y

G

S

S

G

G

H

H

P

P

V

V

A

A

A

T

A

A

V

V

A

A

L

L

E

E

T

N

L

L

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K

L

I

Y

I

Q

E

E

E

M

E

H

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P

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Q

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T

A

E

A

E

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M

G

G

R

Q

L

L

L

L

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R

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Q

G

L

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E

Q

P

H

L

F

L

I

A

D

H

H

P

P

L

L

I

V

G

G

E

E

V

I

R

R

G

G

I

C

G

G

L

L

V

I

A

A

G

A

L

V

E

E

L

L

V

V

V

G

D

D

K

R

H

V

T

S

R

K

A

A

P

P

F

Y

P

Q

R

A

E

L

I

G

A

T

I

L

G

G

V

R

Q

Y

V

M

E

A

R

G

A

R

A

A

R

Q

E

E

Q

H

G

G

L

M

I

I

V

T

R

R

N

A

M

M

G

G

D

D

S

A

I

V

A

A

L

F

A

C

P

P

P

P

F

L

I

I

I

V

S

N

A

E

E

Q

E

E

I

V

V

G

E

M

L

I

V

V

A

E

R

R

L

F

T

A

R

R

A

A

L

L

D

D

active site: Y15 (≠ Q24), Y146 (≠ F155), D253 (= D263), K282 (= K292)
binding pyridoxal-5'-phosphate: S112 (= S121), G113 (= G122), S114 (= S123), Y146 (≠ F155), H147 (= H156), E220 (= E230), D253 (= D263), V255 (≠ I265), I256 (= I266), K282 (= K292)

5ghgB Transaminase w58l with smba
50% identity, 95% coverage: 16:455/463 of query aligns to 8:427/433 of 5ghgB

query
sites
5ghgB

D

D

M

I

R

R

F

Y

H

H

L

L

H

H

S

S

Q
x
Y

T

T

N

D

L

A

R

V

A

R

H

L

Q

E

Q

A

H

E

G

G

P

P

L

L

V

V

I

I

E

E

R

R

G

G

S

D

G

G

I

I

Y

Y

V

V

T

E

D

D

E

V

H

S

G

G

R

K

D

R

Y

Y

I

I

E

E

G

A

M

M

S

S

G

G

L

L

W

L

C

S

A

V

G

G

L

V

G

G

F

F

S

S

N

E

P

P

R

R

L

L

V

A

Q

E

A

A

A

A

Q

A

R

R

Q

Q

M

M

A

K

V

K

L

L

P

P

Y

F

Y

Y

H

H

T

T

F

F

N

H

H

-

R

-

V

-

P

-

N

G

V

P

V

V

A

I

Q

D

L

L

A

A

E

E

R

K

I

L

A

V

S

S

L

M

V

A

P

P

F

V

E

P

K

M

P

S

K

K

V

A

F

Y

F

F

A

T

C

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

S

T

M

V

V

V

K

K

L

L

A

I

W

W

A

Y

Y

R

H

S

R

N

A

A

R

L

G

G

N

E

D

P

G

E

K

R

R

K

K

K

I

I

I

I

A

S

H

R

Q

K

K

R

G

G

F
x
Y

H
|
H

G

G

S

V

T

T

V

I

M

A

G

S

A

A

S

S

L

L

S

T

G

G

L

L

P

P

N

N

M

N

H

H

A

R

A

S

F

F

G

D

L

L

P

P

L

I

E

D

N

R

S

-

V

I

L

L

H

H

V

T

Q

G

C

C

P

P

H

H

F

F

Y

Y

R

R

H

E

G

G

I

Q

E

A

G

G

E

E

S

S

E

E

A

E

Q

Q

F

F

T

A

E

T

R

R

L

L

L

A

R

D

D

E

L

L

E

E

Q

Q

R

L

I

I

E

I

A

A

E

E

G

G

A

P

D

H

T

T

I

I

A

A

A

A

F

F

I

I

S

G

E
|
E

P

P

V

V

M

M

G

G

A

A

G

G

G

G

V

V

I

V

V

V

P

P

P

P

P

K

G

T

Y

Y

F

W

V

E

G

K

V

V

Q

Q

A

A

L

V

L

L

K

K

K

R

H

Y

D

D

I

I

L

L

F

L

L

I

A

A

D
|
D

E

E

I
x
V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

R

N

W

L

F

F

G

G

H

S

Q

Q

T

T

L

F

G

D

F

M

T

K

P

P

D

D

M

I

M

L

A

V

C

M

A

S

K
|
K

S

Q

L

L

S

S

S

S

G

S

Y

Y

Q

L

P

P

I

I

S

S

C

A

V

F

V

L

V

I

A

N

E

E

N

R

V

V

Y

Y

Q

A

V

P

I

I

E

A

E

-

Q

-

S

-

Q

-

Q

-

L

-

G

-

G

-

F

-

G

-

H

-

G

-

F

-

T

-

Y

-

S

S

G

G

H

H

P

P

V

V

A

A

A

A

A

A

V

V

A

A

L

L

E

E

T

N

L

L

T

A

L

I

Y

I

Q

E

E

E

M

R

H

D

L

L

P

V

Q

A

R

N

T

A

E

R

E

D

L

R

G

G

R

T

L

Y

L

M

H

Q

Q

K

Q

R

L

L

E

R

P

E

L

L

L

Q

A

D

H

H

P

P

L

L

I

V

G

G

E

E

V

V

R

R

G

G

I

V

G

G

L

L

V

I

A

A

G

G

L

V

E

E

L

L

V

V

V

T

D

D

K

K

H

Q

T

A

R

K

A

T

P

G

F

L

P

E

R

P

E

T

I

G

A

A

I

L

G

G

V

A

Q

K

V

A

E

N

R

A

A

V

A

L

R

Q

E

E

Q

R

G

G

L

V

I

I

V

S

R

R

N

A

M

M

G

G

D

D

S

T

I

L

A
|
A

L

F

A

C

P

P

P

P

F

L

I

I

I

I

S

N

A

D

E

Q

E

Q

I

V

V

D

E

T

L

M

V

V

A

S

R

A

L

L

T

E

R

A

A

T

L

L

D

N

A

D

V

V

active site: Y16 (≠ Q24), Y143 (≠ F155), E217 (= E230), D250 (= D263), I253 (= I266), K279 (= K292), A401 (= A429)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S109 (= S121), G110 (= G122), S111 (= S123), Y143 (≠ F155), H144 (= H156), E217 (= E230), D250 (= D263), V252 (≠ I265), I253 (= I266), K279 (= K292)

D6R3B6 Vanillin aminotransferase; Putative aminotransferase; pAMT; EC 2.6.1.119 from Capsicum frutescens (Cayenne pepper) (Tabasco pepper) (see paper)
47% identity, 90% coverage: 35:452/463 of query aligns to 27:444/459 of D6R3B6

query
sites
D6R3B6

P

P

L

L

V

V

I

I

E

E

R

K

G

S

S

K

G

G

I

S

Y

Y

V

V

T

Y

D

D

E

I

H

N

G

G

R

K

D

K

Y

Y

I

L

E

D

G

T

M

L

S

S

G

G

L

L

W

W

C

C

A

A

G

T

L

L

G

G

F

G

S

S

N

E

P

T

R

R

L

L

V

V

Q

E

A

A

A

A

Q

N

R

K

Q

Q

M

L

A

N

V

T

L

L

P

P

Y

F

Y

Y

H

H

T

S

F

F

N

W

H

N

R

R

V

T

P

T

N

K

V

P

V

S

A

L

Q

D

L

L

A

A

E

K

R

E

I

L

A

L

S

N

L

M

V

F

P

T

F

A

E

N

K

K

-

M

P

A

K

K

V

V

F

F

F

F

A

T

C

N

S

S

G

G

S

S

E

E

A

A

N

N

D

D

S

T

M

Q

V

V

K

K

L

L

A

V

W

W

A

Y

Y

Y

H

N

R

N

A

A

R

L

G

G

N

R

D

P

G

Q

K

K

R

K

K

K

I

I

I

I

A

A

H

R

Q

A

K

K

G

A

F

Y

H

H

G

G

S

S

T

T

V

Y

M

I

G
x
S

A
|
A

S
x
G

L
|
L

S

S

G

G

L

L

P

P

N

P

M

M

H

H

A

Q

A

K

F

F

G

D

L

L

P

P

L

-

E

P

N

P

S

F

V

V

L

L

H

H

V

T

Q

E

C

C

P

P

H

H

F

Y

Y

W

R

A

H

Y

G

H

I

L

E

P

G

G

E

E

S

T

E

E

A

E

Q

E

F

F

T

S

E

T

R

R

L

L

L

A

R

N

D

N

L

L

E

E

Q

S

R

L

I

I

E

L

A

N

E

E

G

G

A

P

D

E

T

T

I

V

A

A

A

A

F

F

I

I

S

A

E

E

P

P

V

V

M

L

G

G

A

A

G

A

G

G

V

V

I

I

V

L

P

P

P

P

P

A

G

T

Y

Y

F

F

V

D

G

K

V

V

Q

Q

A

A

L

I

L

L

K

R

K

K

H

H

D

D

I

I

L

L

F

F

L

I

A

A

D

D

E

E

I

V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

R

T

W

M

F

F

G

G

H

S

Q

D

T

K

L

Y

G

N

F

I

T

K

P

P

D

D

M

L

M

V

A

S

C

V

A

A

K

K

S

A

L

L

S

S

S

S

G

G

Y

Y

Q

M

P

P

I

I

S

A

C

A

V

V

V

L

V

V

A

S

E

Q

N

K

V

I

Y

S

Q

S

V

V

I

I

E

L

E

S

Q

E

S

S

Q

N

Q

K

L

I

G

G

G

A

F

F

G

C

H

H

G

G

F

F

T

T

Y

Y

S

S

G

G

H

H

P

P

V

V

A

A

A

C

A

A

V

V

A

A

L

L

E

E

T

A

L

L

T

K

L

I

Y

Y

Q

K

E

E

M

R

H

N

L

I

P

T

Q

E

R

V

T

V

E

N

E

K

L

I

G

S

R

Q

L

K

L

F

H

Q

Q

E

Q

G

L

L

E

K

P

A

L

F

L

A

A

D

H

S

P

P

L

I

I

I

G

G

E

E

V

I

R

R

G

G

I

T

G

G

L

L

V

A

A

L

G

S

L

T

E

E

L

F

V

V

V

D

D

N

K

K

H

S

T

P

R

N

A

D

P

P

F

F

P

P

R

Y

E

E

I

W

A

A

I

V

G

G

V

T

Q

Y

V

F

E

G

R

A

A

Q

A

C

R

A

E

K

Q

Y

G

G

L

M

I

L

V

V

R

S

N

S

M

T

G

G

D

D

S

H

I

V

A

N

L

M

A

A

P

P

P

P

F

F

I

T

I

L

S

S

A

L

E

E

E

E

I

L

V

D

E

E

L

L

V

I

A

R

R

I

L

Y

T

G

R

K

A

A

L

L

SAGL 155:158 (≠ GASL 162:165) mutation Missing: Low pungency phenotype, characterized by low levels of capsaicinoids, and accumulation of high levels of capsinoids, which are non-pungent capsaicinoid analogs.

Q94CE5 Gamma-aminobutyrate transaminase POP2, mitochondrial; AtGABA-T; Gamma-aminobutyric acid aminotransferase 1; Protein HEXENAL RESPONSE 1; Protein POLLEN-PISTIL INCOMPATIBILITY 2; AtPOP2; EC 2.6.1.96 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
46% identity, 91% coverage: 35:455/463 of query aligns to 70:491/504 of Q94CE5

query
sites
Q94CE5

P

P

L

L

V

V

I

I

E

A

R

K

G

S

S

E

G

G

I

S

Y

Y

V

V

T

Y

D

D

E

D

H

T

G

G

R

K

D

K

Y

Y

I

L

E

D

G

S

M

L

S

A

G

G

L

L

W

W

C
|
C

A

T

G

A

L

L

G

G

F
x
G

S

N

N

E

P

P

R

R

L

L

V

V

Q

S

A

A

A

A

Q

V

R

E

Q

Q

M

L

A

N

V

T

L

L

P

P

Y

F

Y

Y

H

H

T

S

F

F

N

W

H

N

R

R

V

T

P

T

N

K

V

P

V

S

A

L

Q

D

L

L

A

A

E

K

R

V

I

L

A

L

S

E

L

M

V

F

P

T

F

A

E

N

K

K

-

M

P

A

K

K

V

A

F

F

F

F

A

T

C

S

S

G

G

G

S

S

E

D

A

A

N

N

D

D

S

T

M

Q

V

V

K

K

L

L

A

V

W

W

A

Y

Y

Y

H

N

R

N

A

A

R

L

G

G

N

R

D

P

G

E

K

K

R

K

K

K

I

F

I

I

A

A

H

R

Q

K

K

K

G

S

F

Y

H

H

G

G

S

S

T

T

V

L

M

I

G

S

A

A

S

S

L

L

S

S

G

G

L

L

P

P

N

P

M

L

H

H

A

Q

A

N

F

F

G

D

L

L

P

P

L

A

E

P

N

-

S

F

V

V

L

L

H

H

V

T

Q

D

C

C

P

P

H

H

F

Y

Y

W

R

R

H

F

G

H

I

L

E

P

G

G

E

E

S

T

E

E

A

E

Q

E

F

F

T

S

E

T

R

R

L

L

L

A

R

K

D

N

L

L

E

E

Q

D

R

L

I

I

E

I

A

K

E

E

G

G

A

P

D

E

T

T

I

I

A

G

A

A

F

F

I

I

S

A

E

E

P

P

V

V

M

M

G

G

A

A

G
|
G

G

G

V

V

I

I

V

P

P

P

P

P

P

A

G

T

Y

Y

F

F

V

E

G

K

V

V

Q

Q

A

A

L

V

L

V

K

K

K

K

H

Y

D

D

I

I

L

L

F

F

L

I

A

A

D

D

E

E

I

V

I

I

C

C

G

A

F

F

G

G

R

R

T

L

G

G

R

T

W

M

F

F

G
|
G

H

C

Q

D

T

K

L

Y

G

N

F

I

T

K

P

P

D

D

M

L

M

V

A

T

C

L

A

A

K

K

S

A

L

L

S

S

S

S

G

A

Y

Y

Q

M

P

P

I

I

S

G

C

A

V

I

V

L

V

M

A

S

E

Q

N

E

V

V

Y

A

Q

D

V

V

I

I

E

N

E

S

Q

H

S

S

Q

S

Q

K

L

L

G

G

G

V

F

F

G

S

H

H

G

G

F

F

T

T

Y

Y

S

S

G

G

H

H

P

P

V

V

A

S

A

C

A

A

V

V

A

A

L

I

E

E

T

A

L

L

T

K

L

I

Y

Y

Q

K

E

E

M

R

H

N

L

I

P

P

Q

E

R

Y

T

V

E

A

E

K

L

V

G

A

R

P

L

R

L

F

H

Q

Q

D

Q

G

L

V

E

K

P

A

L

F

L

A

A

S

-

G

H

S

P

P

L

I

I

I

G

G

E

E

V

T

R

R

G

G

I

T

G

G

L

L

V

I

A

L

G

G

L

T

E

E

L

F

V

V

V

D

D

N

K

K

H

S

T

P

R

N

A

E

P

P

F

F

P

P

R

P

E

E

I

W

A

G

I

V

G

G

V

A

Q

F

V

F

E

G

R

A

A

E

A

C

R

Q

E

K

Q

H

G

G

L

M

I

L

V

V

R

R

N

V

M

A

G

G

D

D

S

G

I

I

A

L

L

M

A

S

P

P

P

P

F

L

I

I

I

I

S

S

A

P

E

E

E

E

I

I

V

D

E

E

L

L

V

I

A

S

R

I

L

Y

T

G

R

K

A

A

L

L

D

K

A

A

V

T

C98 (= C63) mutation to Y: In her1-1; loss of E2-hexenal responses.
G103 (≠ F68) mutation to N: In her1-2; loss of E2-hexenal responses.
G271 (= G236) mutation to E: In pop2-6; Loss of activity and complete sterility.
G312 (= G277) mutation to D: In pop2-7; loss of activity.

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
43% identity, 97% coverage: 9:456/463 of query aligns to 5:454/458 of 5kr3A

query
sites
5kr3A

L

L

T

D

E

Q

L

L

A

F

R

E

T

M

D

D

M

R

R

A

F

H

H

F

L

M

H

H

S

P

Q

S

T

T

N

H

L

A

R

H

A

D

H

H

Q

A

Q

S

H

-

G

G

P

A

L

L

-

P

-

G

-

R

V

I

I

I

E

T

R

G

G

G

S

K

G

G

I

I

Y

R

V

I

T

E

D

D

E

H

H

E

G

G

R

R

D

E

Y

Y

I

I

E

D

G

A

M

F

S

A

G

G

L

L

W

Y

C

C

A

V

G

N

L

I

G

G

F

Y

S

G

N

R

P

T

R

E

L

V

V

A

Q

D

A

A

A

I

Q

Y

R

E

Q

Q

M

A

A

K

V

Q

L

L

P

A

Y

Y

Y

Y

H

H

T

T

F

Y

N

V

H

G

R

H

V

S

P

T

N

D

V

A

V

I

A

I

Q

E

L

L

A

S

E

S

R

R

I

I

A

I

-

R

S

D

L

W

V

A

P

P

F

A

E

G

K

M

P

K

K

K

V

V

F

Y

F

Y

A

G

C

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

S

T

M

Q

V

I

K

K

L

L

A

V

W

W

A

Y

Y

Y

H

N

R

N

A

V

R

L

G

G

N

R

D

P

G

N

K

K

R

K

K

K

I

I

I

I

A

S

H

R

Q

E

K

R

G

G

F
x
Y

H
|
H

G

G

S

S

T

G

V

I

M

V

G

T

A

G

S

S

L

L

S

T

G

G

L

L

P

P

N

S

M

F

H

H

A

Q

A

H

F

F

G

D

L

L

P

P

L

I

E

D

N

R

S

-

V

V

L

K

H

H

V

T

Q

V

C

C

P

P

H

H

F

W

Y

Y

R

R

H

A

G

P

I

M

E

-

G

-

E

-

S

S

E

E

A

A

Q

Q

F

F

T

V

E

A

R

Y

L

C

L

V

R

E

D

E

L

L

E

E

Q

K

R

L

I

I

E

A

A

R

E

E

G

G

A

A

D

D

T

T

I

I

A

A

A

A

F

F

I

I

S

A

E
|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

G

V

I

I

I

V

P

P

P

P

P

P

Q

G

G

Y

Y

F

W

V

E

G

A

V

I

Q

Q

A

A

L

V

L

L

K

R

K

K

H

H

D

D

I

I

L

L

F

L

L

I

A

A

D
|
D

E

E

I
x
V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

R

S

W

K

F

M

G

G

H

S

Q

Q

T

H

L

Y

G

G

F

I

T

K

P

P

D

D

M

L

M

I

A

T

C

V

A

A

K
|
K

S

G

L

L

S

T

S

S

G

A

Y

Y

Q

A

P

P

I

L

S

S

C

G

V

V

V

I

V

V

A

G

E

E

N

K

V

V

Y

W

Q

D

V

V

I

I

E

E

E

K

Q

G

S

S

Q

Q

Q

E

L

H

G

G

G

P

F

M

G

G

H

H

G

G

F

W

T

T

Y

Y

S

S

G

G

H

H

P

P

V

I

A

C

A

A

A

A

V

A

A

A

L

L

E

A

T

N

L

L

T

D

L

I

Y

L

Q

E

E

R

M

E

H

N

L

L

P

T

Q

G

R

N

T

A

E

A

E

D

L

V

G

G

R

A

L

Y

L

L

H

Q

Q

Q

Q

R

L

L

-

H

E

E

P

A

L

F

L

G

A

A

H

H

P

P

L

L

I

V

G

G

E

E

V

V

R

R

G

G

I

V

G

G

L

M

V

L

A

A

G

A

L

L

E

E

L

F

V

M

V

A

D

D

K

K

H

D

T

A

R

R

A

T

P

P

F

F

P

D

R

P

E

A

I

L

A

K

I

V

G

G

V

P

Q

K

V

V

E

S

R

A

A

A

A

A

R

L

E

E

Q

D

G

G

L

M

I

I

V

A

R

R

N

A

M

M

-

P

-

H

G

G

D

D

S

I

I

L

A

G

L

F

A

A

P

P

P

P

F

L

I

V

I

T

S

T

A

R

E

A

E

E

I

V

V

D

E

E

L

I

V

V

A

G

R

I

L

A

T

K

R

Q

A

A

L

V

D

D

A

E

V

V

A

A

binding pyridoxal-5'-phosphate: S120 (= S121), G121 (= G122), S122 (= S123), Y154 (≠ F155), H155 (= H156), E225 (= E230), D258 (= D263), V260 (≠ I265), K287 (= K292)

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
43% identity, 96% coverage: 11:456/463 of query aligns to 8:457/460 of 5kr6B

query
sites
5kr6B

E

D

L

L

A

L

R

E

T

M

D

D

M

R

R

A

F

H

H

F

L

F

H

H

S

P

Q

S

T

T

N

H

L

L

R

R

A

D

H

H

Q

A

Q

S

H

-

G

G

P

E

L

L

-

P

-

G

-

R

V

I

I

I

E

T

R

G

G

G

S

K

G

G

I

I

Y

R

V

I

T

Q

D

D

E

S

H

E

G

G

R

R

D

E

Y

Y

I

I

E

D

G

A

M

F

S

A

G

G

L

L

W

Y

C

C

A

V

G

N

L

V

G

G

F

Y

S

G

N

R

P

T

R

E

L

I

V

A

Q

D

A

A

A

I

Q

Y

R

E

Q

Q

M

A

A

K

V

E

L

L

P

A

Y

Y

Y

Y

H

H

T

T

F

Y

N

V

H

G

R

H

V

S

P

N

N

E

V

P

V

I

A

I

Q

E

L

L

A

S

E

E

R

R

I

I

A

I

-

R

S

E

L

W

V

A

P

P

F

A

E

G

K

M

P

S

K

K

V

V

F

Y

F

Y

A

G

C

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

S

T

M

Q

V

I

K

K

L

L

A

V

W

W

A

Y

Y

Y

H

N

R

N

A

V

R

L

G

G

N

R

D

P

G

Q

K

K

R

K

K

K

I

I

I

I

A

S

H

R

Q

Q

K

R

G

G

F
x
Y

H
|
H

G
|
G

S

S

T

G

V

I

M

M

G

T

A

G

S

S

L

L

S

T

G

G

L

L

P

P

N

A

M

F

H

H

A

N

A

H

F

F

G

D

L

L

P

P

L

L

E

E

N

-

S

P

V

I

L

R

H

H

V

T

Q

T

C

C

P

P

H

H

F

Y

Y

Y

R

R

H

-

G

A

I

P

E

A

G

G

E

M

S

S

E

E

A

A

Q

E

F

F

T

S

E

R

R

H

L

C

L

A

R

D

D

E

L

L

E

E

Q

K

R

M

I

I

E

L

A

A

E

E

G

G

A

P

D

D

T

T

I

V

A

A

A

A

F

F

I

I

S

G

E
|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

G

V

I

I

V

V

P

P

P

P

P

P

E

G

G

Y

Y

F

W

V

E

G

A

V

I

Q

Q

A

A

L

V

L

L

K

N

K

K

H

Y

D

D

I

I

L

L

F

L

L

I

A

A

D
|
D

E

E

I
x
V

I

V

C

C

G

G

F

F

G

G

R

R

T

T

G

G

R

S

W

M

F

F

G

G

H

S

Q

H

T

H

L

Y

G

G

F

M

T

K

P

P

D

D

M

L

M

I

A

T

C

V

A

A

K
|
K

S

G

L

L

S

T

S

S

G

A

Y

Y

Q

A

P

P

I

L

S

S

C

G

V

V

V

I

V

V

A

G

E

E

N

K

V

V

Y

W

Q

K

V

V

I

L

E

E

E

Q

Q

G

S

S

Q

D

Q

Q

L

Y

G

G

G

P

F

I

G

G

H

H

G

G

F

W

T

T

Y

Y

S

S

G

G

H

H

P

P

V

I

A

C

A

A

A

A

V

A

A

A

L

L

E

A

T

N

L

L

T

D

L

I

Y

I

Q

E

E

R

M

E

H

N

L

L

P

T

Q

G

R

N

T

A

E

A

E

D

L

T

G

G

R

A

L

Y

L

F

H

Q

Q

Q

Q

R

L

M

-

R

E

E

P

T

L

F

L

G

A

D

H

H

P

P

L

L

I

V

G

G

E

E

V

V

R

R

G

G

I

V

G

G

L

L

V

M

A

A

G

A

L

L

E

E

L

F

V

V

V

A

D

D

K

K

H

D

T

K

R

R

A

T

P

R

F

F

P

D

R

P

E

S

I

L

A

K

I

V

G

G

V

P

Q

R

V

V

E

S

R

A

A

A

A

C

R

L

E

E

Q

D

G

G

L

M

I

I

V

A

R

R

N

A

M

M

-

P

-

H

G

G

D

D

S

I

I

L

A

G

L

F

A

A

P

P

P

P

F

L

I

V

I

I

S

T

A

R

E

A

E

E

I

V

V

D

E

E

L

I

V

V

A

D

R

I

L

A

T

K

R

Q

A

A

L

V

D

D

A

A

V

V

A

A

binding pyridoxal-5'-phosphate: S121 (= S121), G122 (= G122), S123 (= S123), Y155 (≠ F155), H156 (= H156), G157 (= G157), E228 (= E230), D261 (= D263), V263 (≠ I265), K290 (= K292)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
43% identity, 97% coverage: 9:458/463 of query aligns to 2:455/455 of 5kr5A

query
sites
5kr5A

L

L

T

E

E

Q

L

L

A

L

R

E

T

M

D

D

M

R

R

A

F

H

H

F

L

M

H

H

S

P

Q

S

T

T

N

H

L

A

R

Y

A

D

H

H

Q

A

Q

S

H

-

G

G

P

A

L

L

-

P

-

G

-

R

V

I

I

I

E

T

R

G

G

G

S

K

G

G

I

I

Y

R

V

I

T

E

D

D

E

H

H

E

G

G

R

R

D

E

Y

Y

I

I

E

D

G

A

M

F

S

A

G

G

L

L

W

Y

C

C

A

V

G

N

L

I

G

G

F

Y

S

G

N

R

P
x
E

R

E

L

V

V

A

Q
x
D

A

A

A

I

Q

Y

R

E

Q

Q

M

A

A

K

V

Q

L

L

P

A

Y

Y

Y

Y

H

H

T

T

F

Y

N

V

H

G

R

H

V

S

P

N

N

D

V

P

V

V

A

I

Q

E

L

L

A

S

E

S

R

R

I

I

-

I

A

E

S

D

L

W

V

A

P

P

F

A

E

G

K

M

P

K

K

K

V

V

F

F

F

Y

A

G

C

M

S
|
S

G
|
G

S
|
S

E

D

A

A

N

N

D

E

S

T

M

Q

V

I

K

K

L

L

A

V

W

W

A

Y

Y

Y

H

N

R

N

A

V

R

L

G

G

N

R

D

P

G

N

K

K

R

K

K

K

I

I

I

I

A

A

H

R

Q

E

K

R

G

S

F
x
Y

H
|
H

G
|
G

S

S

T

G

V

I

M

V

G

T

A

G

S

S

L

L

S

T

G

G

L

L

P

P

N

S

M

F

H

H

A

Q

A

H

F

F

G

D

L

L

P

P

L

I

E

D

N

R

S

-

V

V

L

K

H

H

V

T

Q

V

C

C

P

P

H

H

F

W

Y

Y

R

-

H

N

G

A

I

P

E

P

G

G

E

M

S

S

E

E

A

A

Q

Q

F

F

T

V

E

A

R

Y

L

C

L

V

R

E

D

E

L

L

E

E

Q

K

R

L

I

I

E

A

A

R

E

E

G

G

A

A

D

D

T

T

I

I

A

A

A

A

F

F

I

I

S

A

E
|
E

P

P

V

V

M

M

G

G

A

T

G

G

G

G

V

I

I

V

V

P

P

P

P

P

P

Q

G

G

Y

Y

F

W

V

E

G

A

V

I

Q

Q

A

A

L

V

L

L

K

R

K

K

H

H

D

D

I

I

L

L

F

L

L

I

A

A

D
|
D

E

E

I
x
V

I

V

C

C

G

G

F

F

G

G

R

R

T

L

G

G

R

S

W

K

F

T

G

G

H

S

Q

E

T

H

L

Y

G

G

F

I

T

K

P

P

D

D

M

L

M

I

A

T

C

V

A

A

K
|
K

S

G

L

L

S

T

S

S

G

A

Y

Y

Q

A

P

P

I

L

S

S

C

A

V

V

V

I

V

V

A

S

E

E

N

K

V

V

Y

W

Q

D

V

V

I

I

E

E

E

K

Q

G

S

S

Q

R

Q

E

L

H

G

G

G

V

F

M

G

G

H

H

G

G

F
x
W

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

V

A

C

A

A

A

A

V

A

A

A

L

L

E

A

T

N

L

L

T

D

L

I

Y

L

Q

E

E

R

M

E

H

N

L

L

P

T

Q

G

R

N

T

A

E

A

E

D

L

V

G

G

R

A

L

Y

L

L

H

Q

Q

Q

Q

R

L

L

-

H

E

E

P

A

L

F

L

G

A

A

H

H

P

P

L

L

I

V

G

G

E

E

V

V

R

R

G

G

I

V

G

G

L

M

V

L

A

A

G

A

L

L

E

E

L

F

V

M

V

A

D

D

K

K

H

G

T

A

R

R

A

T

P

P

F

F

P

D

R

P

E

A

I

L

A

K

I

V

G

S

V

Q

Q

K

V

V

E

A

R

A

A

A

A

A

R

L

E

E

Q
x
D

G

G

L

L

I

I

V

V

R

R

N

A

M

L

-

P

-

H

G

G

D

D

S

I

I

L

A

G

L

F

A

A

P

P

P

P

F

L

I

V

I

T

S

T

A

R

E

A

E

E

I

V

V

D

E

E

L

I

V

V

A

A

R

I

L

A

T

K

R
x
E

A

A

L

F

D

D

A

E

V

V

A

A

L

D

A

A

binding calcium ion: E66 (≠ P71), D70 (≠ Q75), D412 (≠ Q417), E447 (≠ R450)
binding pyridoxal-5'-phosphate: S117 (= S121), G118 (= G122), S119 (= S123), Y151 (≠ F155), H152 (= H156), G153 (= G157), E224 (= E230), D257 (= D263), V259 (≠ I265), K286 (= K292), W322 (≠ F328), T323 (= T329)

4grxC Structure of an omega-aminotransferase from paracoccus denitrificans (see paper)
41% identity, 94% coverage: 19:455/463 of query aligns to 10:444/449 of 4grxC

query
sites
4grxC

F

Y

H

S

L

L

H

Y

S

G

Q
x
F

T

T

N

D

L

M

R

P

A

S

H

V

Q

H

Q

Q

H

R

G

G

P

T

L

V

V

V

I

V

E

T

R

H

G

G

S

E

G

G

I

P

Y

Y

V

I

T

V

D

D

E

V

H

H

G

G

R

R

D

R

Y

Y

I

L

E

D

G

A

M

N

S

S

G

G

L
|
L

W

W

C

N

A

M

G

V

L

A

G

G

F

F

S

D

N

H

P

K

R

G

L

L

V

I

Q

E

A

A

A

A

Q

K

R

A

Q

Q

M

Y

A

D

V

R

L

F

P

P

Y

G

Y

Y

H

H

T

A

F

F

N

F

H

G

R

R

V

M

P

S

N

D

V

Q

V

T

A

V

Q

M

L

L

A

S

E

E

R

K

I

L

A

V

S

E

L

V

V

S

P

P

F

F

E

D

K

N

P

G

K

R

V

V

F

F

F

Y

A

T

C

N

S

S

G

G

S

S

E

E

A

A

N

N

D

D

S

T

M

M

V

V

K

K

L

M

A

L

W

W

A

F

Y

L

H

H

R

A

A

A

R

E

G

G

N

K

D

P

G

Q

K

K

R

R

K

K

I

I

I

L

A

T

H

R

Q

W

K

N

G

A

F
x
Y

H

H

G

G

S

V

T

T

V

A

M

V

G

S

A

A

S

S

L

M

S

T

G

G

L

K

P

P

N

-

M

Y

H

N

A

S

A

V

F

F

G

G

L

L

P

P

L

L

E

P

N

G

S

-

V

F

L

I

H

H

V

L

Q

T

C

C

P

P

H

H

F

Y

Y

W

R

R

H

Y

G

G

I

E

E

E

G

G

E

E

S

T

E

E

A

A

Q

Q

F

F

T

V

E

A

R

R

L

L

L

A

R

R

D

E

L

L

E

E

Q

D

R

T

I

I

E

T

A

R

E

E

G

G

A

A

D

D

T

T

I

I

A

A

A

G

F

F

I

F

S

A

E
|
E

P

P

V

V

M

M

G

G

A

A

G

G

G

G

V

V

I

I

V

P

P

P

P

A

P

K

G

G

Y

Y

F

F

V

Q

G

A

V

I

Q

L

A

P

L

I

L

L

K

R

K

K

H

Y

D

D

I

I

L

P

F

M

L

I

A

S

D
|
D

E

E

I

V

I
|
I

C

C

G

G

F

F

G

G

R

R

T

T

G

G

R

N

W

T

F

W

G

G

H

C

Q

L

T

T

L

Y

G

D

F

F

T

M

P

P

D

D

M

A

M

I

A

I

C

S

A

S

K
|
K

S

N

L

L

S

T

S

A

G

G

Y

F

Q

F

P

P

I

M

S

G

C

A

V

V

V

I

V

L

A

G

E

P

N

D

V

L

Y

A

Q

K

V

R

I

V

E

E

E

A

Q

A

S

V

Q

E

Q

A

L

I

G

E

G

E

F

F

G

P

H

H

G

G

F

F

T
|
T

Y

A

S

S

G

G

H

H

P

P

V

V

A

G

A

C

A

A

V

I

A

A

L

L

E

K

T

A

L

I

T

D

L

V

Y

V

Q

M

E

N

M

E

H

G

L

L

P

A

Q

E

R

N

T

V

E

R

E

R

L

L

G

A

R

P

L

R

L

F

H

E

Q

A

Q

G

L

L

E

K

P

R

L

I

L

A

A

D

H

R

P

P

L

N

I

I

G

G

E

E

V

Y

R

R

G

G

I

I

G

G

L

F

V

M

A

W

G

A

L

L

E

E

L

A

V

V

V

K

D

D

K

K

H

P

T

T

R

K

A

T

P

P

F

F

P

D

R

A

E

N

I

L

A

S

I

V

G

S

V

E

Q

R

V

I

E

A

R

N

A

T

A

C

R

T

E

D

Q

L

G

G

L

L

I

I

V

C

R

R

N

P

M

L

G

G

D

Q

S

S

I

I

A
x
V

L

L

A

C

P

P

P

P

F

F

I

I

I

L

S

T

A

E

E

A

E

Q

I

M

V

D

E

E

L

M

V

F

A

E

R

K

L

L

T

E

R

K

A

A

L

L

D

D

A

K

V

V

active site: F15 (≠ Q24), Y146 (≠ F155), E219 (= E230), D252 (= D263), I255 (= I266), K281 (= K292), T318 (= T329), V418 (≠ A429)
binding delta-amino valeric acid: F15 (≠ Q24), L52 (= L61), Y146 (≠ F155), K281 (= K292)

4e3qA Pmp-bound form of aminotransferase crystal structure from vibrio fluvialis (see paper)
41% identity, 94% coverage: 19:455/463 of query aligns to 13:447/452 of 4e3qA

query
sites
4e3qA

F

Y

H

S

L

L

H

Y

S

G

Q
x
F

T

T

N

D

L

M

R

P

A

S

H

L

Q

H

Q

Q

H

R

G

G

P

T

L

V

V

V

I

V

E

T

R

H

G

G

S

E

G

G

I

P

Y

Y

V

I

T

V

D

D

E

V

H

N

G

G

R

R

D

R

Y

Y

I

L

E

D

G

A

M

N

S

S

G

G

L

L

W

W

C

N

A

M

G

V

L

A

G

G

F

F

S

D

N

H

P

K

R

G

L

L

V

I

Q

D

A

A

A

A

Q

K

R

A

Q

Q

M

Y

A

E

V

R

L

F

P

P

Y

G

Y

Y

H

H

T

A

F

F

N

F

H

G

R

R

V

M

P

S

N

D

V

Q

V

T

A

V

Q

M

L

L

A

S

E

E

R

K

I

L

A

V

S

E

L

V

V

S

P

P

F

F

E

D

K

S

P

G

K

R

V

V

F

F

F

Y

A

T

C

N

S
|
S

G
|
G

S
|
S

E

E

A

A

N

N

D

D

S

T

M

M

V

V

K

K

L

M

A

L

W

W

A

F

Y

L

H

H

R

A

A

A

R

E

G

G

N

K

D

P

G

Q

K

K

R

R

K

K

I

I

I

L

A

T

H

R

Q

W

K

N

G

A

F
x
Y

H
|
H

G

G

S

V

T

T