SitesBLAST

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
46% identity, 96% coverage: 8:242/244 of query aligns to 5:241/244 of P0AEK2

query
sites
P0AEK2

G

G

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A

F

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G

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x
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S

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x
R

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A

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K

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N

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A

A

E

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A

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A

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G

-

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N

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x
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F

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N

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A

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N

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E

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Y

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N

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F

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K

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GASR 12:15 (≠ GGSK 15:18) binding
T37 (≠ Q40) binding
NV 59:60 (≠ DV 60:61) binding
N86 (= N83) binding
Y151 (= Y148) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (= YAAAK 148:152) binding
A154 (= A151) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (= K152) mutation to A: Defect in the affinity for NADPH.
I184 (≠ V181) binding
E233 (= E234) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.

1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
46% identity, 96% coverage: 8:242/244 of query aligns to 4:240/243 of 1q7bA

query
sites
1q7bA

G

G

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A

A

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S

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G

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active site: G15 (= G19), E101 (≠ Q99), S137 (= S135), Q147 (≠ G145), Y150 (= Y148), K154 (= K152)
binding calcium ion: E232 (= E234), T233 (= T235)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G15), S13 (= S17), R14 (≠ K18), T36 (≠ Q40), N58 (≠ D60), V59 (= V61), N85 (= N83), A86 (= A84), G87 (= G85), I88 (≠ Y86), S137 (= S135), Y150 (= Y148), K154 (= K152), P180 (= P178), G181 (= G179), I183 (≠ V181)

P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
45% identity, 98% coverage: 5:242/244 of query aligns to 2:241/244 of P0A2C9

query
sites
P0A2C9

A

S

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C

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M125 (= M122) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
A223 (= A224) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
S224 (= S225) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
44% identity, 97% coverage: 6:242/244 of query aligns to 6:244/247 of 3op4A

query
sites
3op4A

L

L

T

E

G

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G

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S

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x
R

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I

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R

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A

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Q

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A

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I

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G

G

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D

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N

G

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A

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x
N

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N

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N

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N

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A

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x
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N

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N

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M

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R

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G

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T

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x
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K

A

A

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D

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T

K

A

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T

-

L

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F

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L

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M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G15), S17 (= S17), R18 (≠ K18), I20 (= I20), T40 (≠ Q40), N62 (≠ D60), V63 (= V61), N89 (= N83), A90 (= A84), I92 (≠ Y86), V139 (= V133), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (= G179), I187 (≠ V181), T189 (= T183), M191 (≠ L185)

1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
45% identity, 96% coverage: 8:242/244 of query aligns to 4:240/243 of 1q7cA

query
sites
1q7cA

G

G

Q

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I

A

A

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T

G
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G

G

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S

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x
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G

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A

A

I

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A

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L

L

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G

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A

K

K

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V

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A
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T

D

S

M

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N

S

G

A

A

E

Q

R

A

L

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A

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D

D

E

Y

V

L

G

G

-

A

-

N

G

G

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K

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L
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L

D
x
N

V
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V

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T

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D

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V

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-

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-

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-

F

G

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P

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L

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V

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N

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G

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x
I

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M

T

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T

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N

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S

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F

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S

H

K

A

A

A

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M

P

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A

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M

M

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M

A

K

G

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Y

H

G

G

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R

I

I

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I

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G

S
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S

E

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A

V

G

G

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T

Q

M

G

G

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N

K

G

G

G

G
x
Q

A

A

V

N

Y
x
F

A

A

K
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K

G

A

G

G

V

L

I

I

A

G

F

F

S

S

K

K

S

S

I

L

A

A

R

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E

E

N

V

A

A

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S

Y

R

G

G

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V

V

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N

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A

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P

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G

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F

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D

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K

A

A

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D

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Q

G

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E

A

K

G

L

I

L

L

A

A

A

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M

V

E

P

A

A

S

-

T

-

L

-

V

-

G

G

R

R

L

L

G

G

E

G

P

A

N

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E

E

V

I

A

A

A

N

A

A

V

V

V

A

F

F

L

L

A

A

S

S

R

D

D

E

A

A

S

A

F

Y

I

I

T

T

G

G

E

E

T

T

L

L

G

H

V

V

S

N

G

G

M

M

active site: G15 (= G19), S137 (= S135), Q147 (≠ G145), F150 (≠ Y148), K154 (= K152)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G15), S13 (= S17), R14 (≠ K18), A35 (= A39), T36 (≠ Q40), L57 (= L59), N58 (≠ D60), V59 (= V61), G87 (= G85), I88 (≠ Y86)

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
44% identity, 97% coverage: 6:242/244 of query aligns to 6:240/243 of 4i08A

query
sites
4i08A

L

L

T

E

G

G

Q

K

R

V

A

A

F

L

V

V

T

T

G
|
G

G

A

S
|
S

K
x
R

G
|
G

I
|
I

G

G

E

K

A

A

I

I

V

A

R

E

R

L

L

L

A

A

A

E

E

R

G

G

A

A

K

K

V

V

V

I

I

G

A

T

A

A

Q
x
T

D

S

M

E

I

S

S

G

A

A

E

Q

R

A

L

I

A

S

D

D

E

Y

V

L

G

G

-

D

-

N

G

G

L

K

A

G

V

M

R

A

L

L

D
x
N

V
|
V

T

T

D

N

L

P

L

E

E

S

V

I

E

E

R

A

V

V

V

L

T

K

M

A

L

I

-

T

-

D

-

E

-

F

G

G

P

G

F

V

E

D

I

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

Y

I

D

T

Q

R

H

D

D

N

F

L

T

M

K

R

T

M

T

K

V

E

E

E

Q

E

W

W

R

S

Y

D

L

I

L

M

A

E

V

T

N
|
N

L

L

E

T

G

S

V

I

F

F

A

R

S

L

T

S

H

K

A

A

A

V

L

L

P

R

A

G

M

M

Q

M

A

K

G

R

Y

Q

G

G

R

R

I

I

N

N

V

V

G
|
G

S
|
S

E

V

A

V

G

G

R

T

Q

M

G

G

S

N

K

A

G

G

G
x
Q

A

A

V

N

Y
|
Y

A

A

K
|
K

G

A

G

G

V

V

I

I

A

G

F

F

S

T

K

K

S

S

I

M

A

A

R

R

E

E

N

V

A

A

R

S

Y

R

G

G

I

V

T

T

V

V

N

N

V

T

V

V

A

A

P
|
P

G
|
G

P

F

V

I

D

E

T
|
T

P

-

L

-

R

-

K

-

A

-

L

-

D

D

R

M

G

N

G

D

E

E

K

Q

L

R

L

T

A

A

A

T

M

L

E

-

A

A

S

Q

T

V

L

P

V

A

G

G

R

R

L

L

G

G

E

D

P

P

N

R

E

E

V

I

A

A

A

S

A

A

V

V

V

A

F

F

L

L

A

A

S

S

R

P

D

E

A

A

S

A

F

Y

I

I

T

T

G

G

E

E

T

T

L

L

G

H

V

V

S

N

G

G

M

M

active site: G19 (= G19), N113 (= N107), S141 (= S135), Q151 (≠ G145), Y154 (= Y148), K158 (= K152)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G15), S17 (= S17), R18 (≠ K18), I20 (= I20), T40 (≠ Q40), N62 (≠ D60), V63 (= V61), N89 (= N83), A90 (= A84), G140 (= G134), S141 (= S135), Y154 (= Y148), K158 (= K152), P184 (= P178), G185 (= G179), T189 (= T183)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
40% identity, 98% coverage: 3:242/244 of query aligns to 2:242/244 of 4nbuB

query
sites
4nbuB

M

M

R

S

A

R

L

L

T

Q

G

D

Q

K

R

V

A

A

F

I

V

I

T

T

G
|
G

G

A

S

A

K
x
N

G
|
G

I
|
I

G

G

E

L

A

E

I

A

V

A

R

R

R

V

L

F

A

M

A

K

E

E

G

G

A

A

K

K

V

V

I

I

A

A

A
x
D

-
x
F

Q

N

D

E

M

A

I

A

S

G

A

K

E

E

R

A

L

V

A

E

D

A

E

N

V

P

G

G

G

V

L

V

A

F

V

I

R

R

L
x
V

D
|
D

V
|
V

T

S

D

D

L

R

L

E

E

S

V

V

E

H

R

R

V

L

V

V

T

E

-

N

-

V

-

A

-

E

M

R

L

F

G

G

P

K

F

I

E

D

I

I

L

L

V

I

N

N

N
|
N

A
|
A

G
|
G

Y
x
I

D

T

Q

R

H
x
D

D

S

F

M

F

L

T

S

K
|
K

T

M

T

T

V

V

E

D

Q

Q

W

F

R

Q

Y

Q

L

V

L

I

A

N

V

V

N
|
N

L

L

E

T

G

G

V

V

F

F

A

H

S

C

T

T

H

Q

A

A

A

V

L

L

P

P

A

Y

M

M

Q

A

A

E

A

Q

G

G

Y

K

G

G

R

K

I

I

N

N

V
x
T

G

S

S
|
S

E

V

A

T

G

G

R

T

Q

Y

G

G

S
x
N

K
x
V

G

G

G
x
Q

A

T

V

N

Y
|
Y

A

A

K
|
K

G

A

G

G

V

V

I

I

A

G

F

M

S

T

K

K

S

T

I

W

A

A

R

K

E

E

N

L

A

A

R

R

Y

K

G

G

I

I

T

N

V

V

N

N

V

A

V

V

A

A

P
|
P

G

G

P
x
F

V
x
T

D

E

T
|
T

P

A

L
x
M

L

V

R

A

K

E

A

V

L

-

D

-

R

-

G

-

G

P

E

E

K

K

L

V

L

I

A

E

A
x
K

M

M

E

K

A

A

S

Q

T

V

L

P

V

M

G

G

R

R

L

L

G

G

E

K

P

P

N

E

E

D

V

I

A

A

A

N

A

A

V

Y

V

L

F

F

L

L

A

A

S

S

R

H

D

E

A

S

S

D

F

Y

I

V

T

N

G

G

E

H

T

V

L

L

G

H

V

V

S

D

G

G

M

I

active site: G18 (= G19), N111 (= N107), S139 (= S135), Q149 (≠ G145), Y152 (= Y148), K156 (= K152)
binding acetoacetyl-coenzyme a: D93 (≠ H89), K98 (= K94), S139 (= S135), N146 (≠ S142), V147 (≠ K143), Q149 (≠ G145), Y152 (= Y148), F184 (≠ P180), M189 (≠ L185), K200 (≠ A200)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G15), N17 (≠ K18), G18 (= G19), I19 (= I20), D38 (≠ A39), F39 (vs. gap), V59 (≠ L59), D60 (= D60), V61 (= V61), N87 (= N83), A88 (= A84), G89 (= G85), I90 (≠ Y86), T137 (≠ V133), S139 (= S135), Y152 (= Y148), K156 (= K152), P182 (= P178), F184 (≠ P180), T185 (≠ V181), T187 (= T183), M189 (≠ L185)

7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
42% identity, 98% coverage: 5:242/244 of query aligns to 1:240/243 of 7emgB

query
sites
7emgB

A

S

L

F

T

E

G

G

Q

K

R

I

A

V

F

L

V

V

T

T

G
|
G

G

A

S
|
S

K
x
R

G

G

I

I

G

G

E

R

A

A

I

I

V

A

R

E

R

T

L

F

A

V

A

A

E

R

G

G

A

A

K

K

V

V

V

I

I

G

A

T

A

A

Q
x
T

D

S

M

E

I

S

S

G

A

A

E

E

R

A

L

I

A

S

D

G

E

Y

V

L

G

G

-

A

-

N

G

G

L

K

A

G

V

F

R

M

L

L

D
x
N

V
|
V

T

K

D

D

L

A

L

Q

E

S

V

I

E

D

R

S

V

V

V

L

T

A

M

S

L

I

-

R

-

A

-

E

-

F

G

G

P

E

F

I

E

D

I

I

L

L

V

V

N

N

A

A

G

G

Y
x
I

D

T

Q

R

H

D

D

N

F

L

T

M

K

R

T

M

T

K

V

D

E

D

Q

E

W

W

R

E

Y

D

L

I

L

L

A

D

V

T

N

N

L

L

E

T

G

S

V

V

F

F

A

R

S

L

T

S

H

K

A

A

A

V

L

M

P

C

A

A

M

M

Q

M

A

K

G

R

Y

F

G

G

R

R

I

I

N

T

V

I

G

G

S

S

E

V

A

V

G

G

R

T

Q

M

G

G

S

N

K

A

G

G

G

Q

A

A

V

N

Y

Y

A

A

K

K

G

A

G

G

V

L

I

I

A

G

F

F

S

S

K

K

S

S

I

L

A

A

R

R

E

E

N

V

A

A

R

S

Y

R

G

G

I

I

T

T

V

V

N

N

V

V

A

A

P

P

G

G

P

Y

V

I

D

E

T

T

P

D

L

M

L

T

R

R

K

A

A

L

L

T

D

D

R

D

G

Q

G

R

E

A

K

G

L

I

L

L

A

S

M

V

E

P

A

A

S

N

T

-

L

-

V

-

G

-

R

R

L

P

G

G

E

D

P

A

N

K

E

E

V

I

A

A

A

S

A

A

V

V

V

A

F

F

L

L

A

A

S

S

R

D

D

E

A

A

S

G

F

Y

I

I

T

T

G

G

E

E

T

T

L

L

G

H

V

V

S

N

G

G

M

M

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G15), S13 (= S17), R14 (≠ K18), T36 (≠ Q40), N58 (≠ D60), V59 (= V61), I88 (≠ Y86)

4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
42% identity, 99% coverage: 1:242/244 of query aligns to 4:250/258 of 4wecA

query
sites
4wecA

M

L

T

T

M

Q

R

R

A

-

L

L

T

A

G

G

Q

K

R

V

A

A

F

V

V

I

T

T

G
|
G

G

G

S
x
A

K
x
S

G
|
G

I
|
I

G

G

E

L

A

A

I

T

V

G

R

R

L

L

A

R

A

A

E

E

G

G

A

A

K

T

V

V

I

V

A

G

A
x
D

Q
x
I

D

D

M

P

I

T

S

T

A

G

E

K

R

A

L

A

A

A

D

D

E

E

V

L

G

E

G

G

L

L

A

F

V

V

R

P

L
x
V

D
|
D

V
|
V

T

S

D

E

L

Q

L

E

E

A

V

V

E

D

R

N

V

L

V

F

-

D

-

T

-

A

T

S

M

T

L

F

G

G

P

R

F

V

E

D

I

I

L

A

V

F

N

N

N
|
N

A

A

G

G

Y

I

D

S

-

P

Q

E

H

D

D

D

F

L

F

I

T

E

K

N

T

T

T

D

V

L

E

P

Q

A

W

W

R

Q

Y

R

L

V

L

Q

A

D

V

I

N

N

L

L

E

K

G

S

V

V

F

Y

A

L

S

S

T

C

H

R

A

A

L

L

P

R

A

H

M

M

Q

V

A

P

A

A

G

G

Y

K

G

G

R

S

I

I

N

N

V
x
T

G

A

S
|
S

E

F

A

V

G

A

R

V

Q

M

G

G

S

S

K

A

G

T

G

S

A
x
Q

V

I

-

S

Y
|
Y

A

T

A

A

A

S

K
|
K

G

G

V

V

I

L

A

A

F

M

S

S

K

R

S

E

I

L

A

G

R

V

E

Q

N

Y

A

A

R

R

Y

Q

G

G

I

I

T

R

V

V

N

N

V

A

V

L

A

C

P
|
P

G

G

P
|
P

V
|
V

D

N

T

T

P

P

L

L

R

Q

K

E

A

L

L

F

D

A

R

K

G

D

G

P

E

E

K

R

L

-

L

A

A

A

A

R

M

R

E

L

A

V

S

H

T

I

L

P

V

L

G

G

R

R

L

F

G

A

E

E

P

P

N

E

E

E

V

L

A

A

V

V

V

A

F

F

L

L

A

A

S

S

R

D

D

D

A

A

S

S

F

F

I

I

T

T

G

G

E

S

T

T

L

F

G

L

V

V

S

D

G

G

M

I

active site: G21 (= G19), S143 (= S135), Q154 (≠ A146), Y157 (= Y148), K161 (= K152)
binding nicotinamide-adenine-dinucleotide: G17 (= G15), A19 (≠ S17), S20 (≠ K18), G21 (= G19), I22 (= I20), D41 (≠ A39), I42 (≠ Q40), V61 (≠ L59), D62 (= D60), V63 (= V61), N89 (= N83), T141 (≠ V133), Y157 (= Y148), K161 (= K152), P187 (= P178), P189 (= P180), V190 (= V181)

4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
42% identity, 98% coverage: 4:242/244 of query aligns to 1:237/239 of 4nbtA

query
sites
4nbtA

R

K

A

K

L

L

T

E

G

G

Q

K

R

V

A

A

F

V

V

I

T

T

G
|
G

G

G

S

A

K
|
K

G
|
G

I
x
L

G

G

E

Q

A

A

I

I

V

A

R

L

R

A

L

Y

A

A

A

E

E

E

G

G

A

A

K

K

V

V

V

I

I

-

A

-

A

A

Q

G

D
|
D

M
x
L

I

G

S

D

A

L

E

T

R

Y

L

S

A

H

D

P

E

N

V

V

G

E

G

G

L

M

A

-

V

-

R

Y

L
|
L

D
x
N

V
|
V

T

T

D

D

L

V

L

T

E

G

V

V

E

E

R

K

-

F

-

Y

-

Q

-

S

V

V

V

I

T

D

M

K

L

Y

G

G

P

K

F

I

E

D

I

I

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

Y

I

D

T

Q

K

H

D

D

A

F

M

F

T

T

R

K

K

T

M

T

T

V

E

E

A

Q

Q

W

W

R

D

Y

A

L

V

L

I

A

D

V

V

N

N

L

L

E

K

G

G

V

V

F

F

A

N

S

L

T

T

H

R

A

L

A

V

L

G

P

P

A

Q

M

M

Q

Q

A

T

A

N

G

G

Y

Y

G

G

R

S

I

I

N

N

V
x
I

G

S

S
|
S

E

V

A

V

G

G

R

V

Q

F

G

G

S

N

K

I

G

G

G

Q

A

A

V

N

Y
|
Y

A

A

A

T

K
|
K

G

A

G

G

V

V

I

I

A

G

F

L

S

T

K

M

S

T

I

W

A

A

R

K

E

E

N

F

A

A

R

L

Y

K

G

G

-

A

-

N

I

V

T

R

V

V

N

N

V

A

V

I

A

A

P
|
P

G
|
G

P

Y

V
x
I

D

M

T
|
T

P

D

L

I

L

L

R

K

K

T

A

V

L

-

D

-

R

-

G

-

G

P

E

Q

K

D

L

L

A

D

A

K

M

F

E

A

A

A

S

L

T

T

L

M

V

L

G

N

R

R

L

L

G

G

E

Q

P

P

N

E

E

E

V

I

A

A

A

K

A

V

V

A

V

L

F

F

L

L

A

A

S

S

R

D

D

D

A

A

S

S

F

Y

I

V

T

T

G

G

E

Q

T

T

L

I

G

N

V

V

S

N

G

G

M

M

active site: G16 (= G19), S132 (= S135), Y145 (= Y148), K149 (= K152)
binding nicotinamide-adenine-dinucleotide: G12 (= G15), K15 (= K18), G16 (= G19), L17 (≠ I20), D36 (= D41), L37 (≠ M42), L52 (= L59), N53 (≠ D60), V54 (= V61), N80 (= N83), A81 (= A84), G82 (= G85), I130 (≠ V133), S132 (= S135), Y145 (= Y148), K149 (= K152), P177 (= P178), G178 (= G179), I180 (≠ V181), T182 (= T183)

5ovlA Crystal structure of maba bound to NADP+ from m. Smegmatis (see paper)
42% identity, 98% coverage: 5:243/244 of query aligns to 6:238/241 of 5ovlA

query
sites
5ovlA

A

A

L

F

T

V

G

S

Q

R

R

S

A

V

F

L

V

V

T

T

G
|
G

G

G

S
x
N

K
x
R

G
|
G

I
|
I

G

G

E

L

A

A

I

I

V

A

R

R

L

L

A

A

E

D

G

G

A

H

K

K

V

V

V

A

I

V

A

T

A

H

Q
x
R

D

G

M

S

I

G

S

A

A

P

E

D

R

D

L

L

A

-

D

-

E

-

V

-

G

-

L

F

A

G

V

V

R

Q

L

C

D
|
D

V
|
V

T

T

D

D

L

S

L

A

E

A

V

V

E

D

R

R

-

A

-

F

-

K

-

E

V

V

V

E

T

E

M

H

L

Q

G

G

P

P

F

V

E

E

I

V

L

L

V

V

N

A

N
|
N

A
|
A

G

G

Y
x
I

D

S

Q

K

H

D

D

A

F

F

F

L

T

M

K

R

T

M

T

T

V

E

E

E

Q

R

W

F

R

E

Y

E

L

V

L

I

A

N

V
x
T

N

N

L

L

E

T

G

G

V

A

F

F

A

R

S

C

T

A

H

Q

A

R

A

A

L

S

P

R

A

T

M

M

Q

Q

A

R

A

K

G

R

Y

F

G

G

R

R

I

I

N

F

V
x
I

G

G

S
|
S

E

V

A

S

G

G

R

M

Q

W

G

G

S

I

K

G

G

N

G

Q

A

A

V

N

Y
|
Y

A

A

K
|
K

G

A

G

G

V
x
L

I

I

A

G

F

M

S

A

K

R

S

S

I

I

A

S

R

R

E

E

N

L

A

A

R

K

Y

A

G

G

I

V

T

T

V

A

N

N

V

V

A

A

P
|
P

G
|
G

P

Y

V
x
I

D

D

T

T

P

E

L

M

L

T

R

R

K

-

A

A

L

L

D

D

R

-

G

-

E

E

K

R

L

I

L

Q

A

A

A

G

M

A

E

L

A

D

S

F

T

I

L

P

V

A

G

K

R

R

L

V

G

G

E

T

P

A

N

E

E

E

V

V

A

A

A

G

A

A

V

V

V

S

F

F

L

L

A

A

S

S

R

E

D

D

A

A

S

S

F

Y

I

I

T

A

G

G

E

A

T

V

L

I

G

P

V

V

S

D

G

G

M

M

G

G

active site: G20 (= G19), S134 (= S135), Y147 (= Y148), L154 (≠ V155)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G15), N18 (≠ S17), R19 (≠ K18), G20 (= G19), I21 (= I20), R41 (≠ Q40), D55 (= D60), V56 (= V61), N82 (= N83), A83 (= A84), I85 (≠ Y86), T105 (≠ V106), I132 (≠ V133), S134 (= S135), Y147 (= Y148), K151 (= K152), P177 (= P178), G178 (= G179), I180 (≠ V181)

P71534 3-oxoacyl-[acyl-carrier-protein] reductase MabA; 3-ketoacyl-acyl carrier protein reductase; Acetoacetyl-CoA reductase; Beta-ketoacyl-ACP reductase; Beta-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100; EC 1.1.1.36 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
42% identity, 98% coverage: 5:243/244 of query aligns to 20:252/255 of P71534

query
sites
P71534

A

A

L

F

T

V

G

S

Q

R

R

S

A

V

F

L

V

V

T

T

G

G

S
x
N

K
x
R

G
|
G

I
|
I

G

G

E

L

A

A

I

I

V

A

R

R

L

L

A

A

E

D

G

G

A

H

K

K

V

V

V

A

I

V

A

T

A

H

Q
x
R

D

G

M

S

I

G

S

A

A

P

E

D

R

D

L

L

A

-

D

-

E

-

V

-

G

-

L

F

A

G

V

V

R

Q

L

C

D
|
D

V
|
V

T

T

D

D

L

S

L

A

E

A

V

V

E

D

R

R

-

A

-

F

-

K

-

E

V

V

V

E

T

E

M

H

L

Q

G

G

P

P

F

V

E

E

I

V

L

L

V

V

N

A

N

N

A

A

G

G

Y

I

D

S

Q

K

H

D

D

A

F

F

F

L

T

M

K

R

T

M

T

T

V

E

E

E

Q

R

W

F

R

E

Y

E

L

V

L

I

A

N

V

T

N

N

L

L

E

T

G

G

V

A

F

F

A

R

S

C

T

A

H

Q

A

R

A

A

L

S

P

R

A

T

M

M

Q

Q

A

R

A

K

G

R

Y

F

G

G

R

R

I

I

N

F

V

I

G

G

S

S

E

V

A

S

G

G

R

M

Q

W

G

G

S

I

K

G

G

N

G

Q

A

A

V

N

Y
|
Y

A

A

K
|
K

G

A

G

G

V

L

I

I

A

G

F

M

S

A

K

R

S

S

I

I

A

S

R

R

E

E

N

L

A

A

R

K

Y

A

G

G

I

V

T

T

V

A

N

N

V

V

A

A

P

P

G

G

P

Y

V
x
I

D

D

T

T

P

E

L

M

L

T

R

R

K

-

A

A

L

L

D

D

R

-

G

-

E

E

K
x
R

L

I

L

Q

A

A

A

G

M

A

E

L

A

D

S

F

T

I

L

P

V

A

G

K

R

R

L

V

G

G

E

T

P

A

N

E

E

E

V

V

A

A

A

G

A

A

V

V

V

S

F

F

L

L

A

A

S

S

R

E

D

D

A

A

S

S

F

Y

I

I

T

A

G

G

E

A

T

V

L

I

G

P

V

V

S

D

G

G

M

M

G

G

NRGI 32:35 (≠ SKGI 17:20) binding
R55 (≠ Q40) binding
DV 69:70 (= DV 60:61) binding
Y161 (= Y148) binding
K165 (= K152) binding
I194 (≠ V181) binding
R205 (≠ K196) binding

5ovkA Crystal structure maba bound to NADPH from m. Smegmatis (see paper)
42% identity, 98% coverage: 5:243/244 of query aligns to 7:239/242 of 5ovkA

query
sites
5ovkA

A

A

L

F

T

V

G

S

Q

R

R

S

A

V

F

L

V

V

T

T

G
|
G

G

G

S
x
N

K
x
R

G
|
G

I

I

G

G

E

L

A

A

I

I

V

A

R

R

L

L

A

A

E

D

G

G

A

H

K

K

V

V

V

A

I

V

A

T

A

H

Q
x
R

D

G

M

S

I

G

S

A

A

P

E

D

R

D

L

L

A

-

D

-

E

-

V

-

G

-

L

F

A

G

V

V

R

Q

L

C

D
|
D

V
|
V

T

T

D

D

L

S

L

A

E

A

V

V

E

D

R

R

-

A

-

F

-

K

-

E

V

V

V

E

T

E

M

H

L

Q

G

G

P

P

F

V

E

E

I

V

L

L

V

V

N

A

N

N

A
|
A

G
|
G

Y

I

D

S

Q

K

H

D

D

A

F

F

F

L

T

M

K

R

T

M

T

T

V

E

E

E

Q

R

W

F

R

E

Y

E

L

V

L

I

A

N

V

T

N

N

L

L

E

T

G

G

V

A

F

F

A

R

S

C

T

A

H

Q

A

R

A

A

L

S

P

R

A

T

M

M

Q

Q

A

R

A

K

G

R

Y

F

G

G

R

R

I

I

N

F

V

I

G

G

S
|
S

E

V

A

S

G

G

R

M

Q

W

G

G

S

I

K

G

G

N

G

Q

A

A

V

N

Y
|
Y

A

A

K

K

G

A

G

G

V

L

I

I

A

G

F

M

S

A

K

R

S

S

I

I

A

S

R

R

E

E

N

L

A

A

R

K

Y

A

G

G

I

V

T

T

V

A

N

N

V

V

A

A

P

P

G

G

P

Y

V

I

D

D

T

T

P

E

L

M

L

T

R

R

K

-

A

A

L

L

D

D

R

-

G

-

E

E

K

R

L

I

L

Q

A

A

A

G

M

A

E

L

A

D

S

F

T

I

L

P

V

A

G

K

R

R

L

V

G

G

E

T

P

A

N

E

E

E

V

V

A

A

A

G

A

A

V

V

V

S

F

F

L

L

A

A

S

S

R

E

D

D

A

A

S

S

F

Y

I

I

T

A

G

G

E

A

T

V

L

I

G

P

V

V

S

D

G

G

M

M

G

G

active site: G21 (= G19), S135 (= S135), Y148 (= Y148)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G17 (= G15), N19 (≠ S17), R20 (≠ K18), R42 (≠ Q40), D56 (= D60), V57 (= V61), A84 (= A84), G85 (= G85)

6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
39% identity, 97% coverage: 6:242/244 of query aligns to 1:241/244 of 6wprA

query
sites
6wprA

L

M

T

T

G

Q

Q

E

R

R

-

K

-

V

A

A

F

L

V

V

T

T

G
|
G

G

A

S
|
S

K
x
R

G
|
G

I

I

G

G

E

A

A

A

I

I

V

A

R

Q

L

L

A

I

A

Q

E

D

G

G

A

Y

K

F

V

V

I

G

A

T

A

A

Q
x
T

D

S

M

E

I

S

S

G

A

A

E

Q

R

K

L

L

A

T

D

D

E

S

V

F

G

G

-

E

-

Q

G

G

L

A

A

G

V

L

R

A

L
|
L

D
|
D

V
|
V

T

R

D

N

L

L

L

D

E

E

V

I

E

E

R

A

V

V

T

S

M

H

L

I

-

E

-

Q

-

N

-

Y

G

G

P

P

F

V

E

L

I

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

Y
x
I

D

T

Q

K

H

D

D

N

F

L

T

L

K

R

T

M

T

S

V

E

E

D

Q

D

W

W

R

D

Y

D

L

I

L

L

A

N

V

I

N

H

L

L

E

K

G

A

V

V

F

Y

A

R

S

L

T

S

H

K

A

R

A

V

L

L

P

K

A

G

M

M

Q

T

A

K

A

A

G

R

Y

F

G

G

R

R

I

I

N

N

V
x
I

G

S

S
|
S

E

V

A

V

G

A

R

H

Q

F

G

A

S

N

K

P

G

G

G

Q

A

A

V

N

Y
|
Y

A

S

A

A

K
|
K

G

A

G

G

V

I

I

E

A

A

F

F

S

S

K

R

S

S

I

L

A

A

R

K

E

E

N

M

A

G

R

S

Y

R

G

Q

I

I

T

T

V

V

N

N

V

S

V

V

A

A

P
|
P

G

G

P

F

V

I

D

A

T

T

P

E

L

M

L

-

R

-

K

-

A

-

L

T

D

D

R

A

G

L

G

S

E

E

K

D

L

I

L

R

A

K

M

M

E

S

A

D

S

Q

T

V

L

A

V

L

G

N

R

R

L

L

G

G

E

E

P

P

N

Q

E

D

V

I

A

A

A

N

A

A

V

V

V

S

F

F

L

L

A

A

S

S

R

D

D

K

A

A

S

G

F

Y

I

I

T

T

G

G

E

T

T

V

L

L

G

H

V

V

S

N

G

G

M

L

active site: G16 (= G19), S138 (= S135), Y151 (= Y148)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G15), S14 (= S17), R15 (≠ K18), T37 (≠ Q40), L58 (= L59), D59 (= D60), V60 (= V61), N86 (= N83), A87 (= A84), G88 (= G85), I89 (≠ Y86), I136 (≠ V133), Y151 (= Y148), K155 (= K152), P181 (= P178)

6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
39% identity, 95% coverage: 11:242/244 of query aligns to 8:241/244 of 6t62A

query
sites
6t62A

A

A

F

L

V

V

T

T

G
|
G

G

A

S
|
S

K
x
R

G
|
G

I

I

G

G

E

A

A

A

I

I

V

A

R

Q

L

L

A

I

A

Q

E

D

G

G

A

Y

K

F

V

V

I

G

A

T

A
|
A

Q
x
T

D

S

M

E

I

S

S

G

A

A

E

Q

R

K

L

L

A

T

D

D

E

S

V

F

G

G

-

E

-

Q

G

G

L

A

A

G

V

L

R

A

L
|
L

D
|
D

V
|
V

T

R

D

N

L

L

L

D

E

E

V

I

E

E

R

A

V

V

T

S

M

H

L

I

-

E

-

Q

-

N

-

Y

G

G

P

P

F

V

E

L

I

V

L

L

V

V

N

N

N
|
N

A
|
A

G
|
G

Y
x
I

D

T

Q

K

H

D

D

N

F

L

T

L

K

R

T

M

T

S

V

E

E

D

Q

D

W

W

R

D

Y

D

L

I

L

L

A

N

V

I

N

H

L

L

E

K

G

A

V

V

F

Y

A

R

S

L

T

S

H

K

A

R

A

V

L

L

P

K

A

G

M

M

Q

T

A

K

A

A

G

R

Y

F

G

G

R

R

I

I

N

N

V
x
I

G
x
S

S
|
S

E

V

A

V

G

A

R

H

Q

F

G

A

S

N

K

P

G

G

G

Q

A

A

V

N

Y
|
Y

A

S

A

A

K
|
K

G

A

G

G

V

I

I

E

A

A

F

F

S

S

K

R

S

S

I

L

A

A

R

K

E

E

N

M

A

G

R

S

Y

R

G

Q

I

I

T

T

V

V

N

N

V

S

V

V

A

A

P
|
P

G
|
G

P

F

V
x
I

D

A

T

T

P

E

L
x
M

L

-

R

-

K

-

A

-

L

T

D

D

R

A

G

L

G

S

E

E

K

D

L

I

L

R

A

K

M

M

E

S

A

D

S

Q

T

V

L

A

V

L

G

N

R

R

L

L

G

G

E

E

P

P

N

Q

E

D

V

I

A

A

A

N

A

A

V

V

V

S

F

F

L

L

A

A

S

S

R

D

D

K

A

A

S

G

F

Y

I

I

T

T

G

G

E

T

T

V

L

L

G

H

V

V

S

N

G

G

M

L

active site: G16 (= G19), S138 (= S135), Y151 (= Y148)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G15), S14 (= S17), R15 (≠ K18), A36 (= A39), T37 (≠ Q40), L58 (= L59), D59 (= D60), V60 (= V61), N86 (= N83), A87 (= A84), G88 (= G85), I89 (≠ Y86), I136 (≠ V133), S137 (≠ G134), S138 (= S135), Y151 (= Y148), K155 (= K152), P181 (= P178), G182 (= G179), I184 (≠ V181), M188 (≠ L185)

P9WGT3 3-oxoacyl-[acyl-carrier-protein] reductase MabA; 3-ketoacyl reductase; 3-ketoacyl-acyl carrier protein reductase; Acetoacetyl-CoA reductase; Beta-ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; Mycolic acid biosynthesis A; EC 1.1.1.100; EC 1.1.1.36 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 7 papers)
41% identity, 95% coverage: 12:243/244 of query aligns to 19:244/247 of P9WGT3

query
sites
P9WGT3

F

L

V

V

T
|
T

G

G

S

N

K
x
R

G
|
G

I
|
I

G

G

E

L

A

A

I

I

V

A

R

Q

R

R

L

L

A

A

E

D

G

G

A

H

K

K

V

V

V

A

I

V

A

T

A

H

Q
x
R

D

G

M

S

I

G

S

A

A

P

E

K

R

G

L

L

A

-

D

-

E

-

V

-

G

-

L

F

A

G

V

V

R

E

L
x
C

D
|
D

V
|
V

T

T

D

D

L

S

L

D

E

A

V

V

E

D

R

R

V

A

V

F

T

T

M

A

L

V

-

E

-

H

-

Q

G

G

P

P

F

V

E

E

I

V

L

L

V

V

N

S

N

N

A

A

G
|
G

Y

L

D

S

Q

A

H

D

D

A

F

F

F

L

T

M

K

R

T

M

T

T

V

E

E

E

Q

K

W

F

R

E

Y

K

L

V

L

I

A

N

V

A

N

N

L

L

E
x
T

G

G

V

A

F

F

A

R

S

V

T

A

H

Q

A

R

A

A

L

S

P

R

A

S

M

M

Q

Q

A

R

A

N

G

K

Y

F

G

G

R

R

I

M

I

I

N

F

V

I

G
|
G

S
|
S

E

V

A

S

G

G

R
x
S

Q

W

G

G

S

I

K

G

G

N

G

Q

A

A

V

N

Y

Y

A

A

A

S

K

K

G

A

G

G

V

V

I

I

A

G

F

M

S

A

K

R

S

S

I

I

A

A

R

R

E

E

N

L

A

S

R

K

Y

A

G

N

I

V

T

T

V

A

N

N

V

V

A

A

P

P

G

G

P
x
Y

V

I

D

D

T

T

P

D

L

M

L
x
T

R

R

K

A

A

L

L

D

D

E

R

R

G

I

G

Q

E

Q

K

G

L

A

L

L

A

Q

A

F

M

I

E

P

A

A

S

K

T

-

L

-

V

-

G

-

R

R

L

V

G

G

E

T

P

P

N

A

E

E

V

V

A

A

A

G

A

V

V

V

V

S

F

F

L

L

A

A

S

S

R

E

D

D

A

A

S

S

F

Y

I

I

T

S

G

G

E

A

T

V

L

I

G

P

V

V

S

D

G

G

M

M

G

G

T21 (= T14) modified: Phosphothreonine; mutation to A: Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-114 and A-191.
RGI 25:27 (≠ KGI 18:20) binding
R47 (≠ Q40) binding
C60 (≠ L59) mutation to V: Displays a lower activity than the wild-type and a slightly decreased affinity for the cofactor. Retains 84% of activity; when associated with L-144. Totally inactive; when associated with A-139 and L-144.
DV 61:62 (= DV 60:61) binding
G90 (= G85) binding
T114 (≠ E109) modified: Phosphothreonine; mutation to A: Slight decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-191.
G139 (= G134) mutation to A: Complete protein inactivation and freezes the catalytic site into its closed form. Totally inactive; when associated with V-60 and L-144.
S140 (= S135) mutation to A: Loss of activity. Can still bind NADPH.; mutation to T: Loss of activity. Impaired NADPH binding.
S144 (≠ R139) mutation to L: Stabilizes the catalytic loop in its open active form. Retains 84% of activity; when associated with V-60. Totally inactive; when associated with V-60 and A-139.
Y185 (≠ P180) mutation to L: 70% decrease in activity with acetoacetyl-CoA as substrate. Does not affect NADP binding.
T191 (≠ L186) modified: Phosphothreonine; mutation to A: Retains 22% of wild-type reductase activity. Strong decrease in phosphorylation by PknB. Lack of phosphorylation by PknB; when associated with A-21 and A-114.; mutation to D: Phosphomimetic mutant that retains less than 10% of wild-type reductase activity. Impaired NADPH binding. Overproduction of the mutant leads to a significant inhibition of de novo biosynthesis of mycolic acids.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1uznA Maba from mycobacterium tuberculosis (see paper)
41% identity, 95% coverage: 12:243/244 of query aligns to 11:236/239 of 1uznA

query
sites
1uznA

F

L

V

V

T

T

G
|
G

G

G

S
x
N

K
x
R

G
|
G

I
|
I

G

G

E

L

A

A

I

I

V

A

R

Q

R

R

L

L

A

A

E

D

G

G

A

H

K

K

V

V

V

A

I

V

A

T

A

H

Q
x
R

D

G

M

S

I

G

S

A

A

P

E

K

R

G

L

L

A

-

D

-

E

-

V

-

G

-

L

F

A

G

V

V

R

E

L

V

D
|
D

V
|
V

T

T

D

D

L

S

L

D

E

A

V

V

E

D

R

R

V

A

V

F

T

T

M

A

L

V

-

E

-

H

-

Q

G

G

P

P

F

V

E

E

I

V

L

L

V

V

N

S

N

N

A
|
A

G
|
G

Y

L

D

S

Q

A

H

D

D

A

F

F

F

L

T

M

K

R

T

M

T

T

V

E

E

E

Q

K

W

F

R

E

Y

K

L

V

L

I

A

N

V

A

N

N

L

L

E

T

G

G

V

A

F

F

A

R

S

V

T

A

H

Q

A

R

A

A

L

S

P

R

A

S

M

M

Q

Q

A

R

A

N

G

K

Y

F

G

G

R

R

I

M

I

I

N

F

V

I

G

G

S
|
S

E

V

A

S

G

G

R

L

Q

W

G

G

S

I

K

G

G

N

G

Q

A

A

V

N

Y
|
Y

A

A

A

S

K
|
K

G

A

G

G

V

V

I

I

A

G

F

M

S

A

K

R

S

S

I

I

A

A

R

R

E

E

N

L

A

S

R

K

Y

A

G

N

I

V

T

T

V

A

N

N

V

V

A

A

P

P

G

G

P

Y

V

I

D

D

T

T

P

D

L

M

L

T

R

R

K

A

A

L

L

D

D

E

R

R

G

I

G

Q

E

Q

K

G

L

A

L

L

A

Q

A

F

M

I

E

P

A

A

S

K

T

-

L

-

V

-

G

-

R

R

L

V

G

G

E

T

P

P

N

A

E

E

V

V

A

A

A

G

A

V

V

V

V

S

F

F

L

L

A

A

S

S

R

E

D

D

A

A

S

S

F

Y

I

I

T

S

G

G

E

A

T

V

L

I

G

P

V

V

S

D

G

G

M

M

G

G

active site: G18 (= G19), S132 (= S135), Y145 (= Y148), K149 (= K152)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G15), N16 (≠ S17), R17 (≠ K18), I19 (= I20), R39 (≠ Q40), D53 (= D60), V54 (= V61), A81 (= A84), G82 (= G85)

P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
41% identity, 98% coverage: 3:242/244 of query aligns to 1:244/247 of P73574

query
sites
P73574

M

M

R

T

A

A

L

L

T

T

G

A

Q

Q

R

V

A

A

F

L

V

V

T

T

G

G

G
x
A

S

S

K

R

G

G

I

I

G

G

E

K

A

A

I

T

V

A

R

L

R

A

L

L

A

A

E

T

G

G

A

M

K

K

V

V

I

V

-

N

-

Y

-

A

-

Q

-

S

A

T

A

A

Q

A

D

D

M

A

I

V

S

V

A

A

E

E

R

I

L

I

A

A

D

N

E

G

V

G

G

E

G

A

L

I

A

A

V

V

R

Q

L

A

D

N

V

V

T

A

D

N

L

A

L

D

E

E

V

V

E

D

R

Q

V

L

V

I

-

K

T

T

M

T

L

L

G

D

P

K

F

F

-

S

-

R

-

I

E

D

I

V

L

L

V

V

N

N

A

A

G

G

Y

I

D

T

Q

R

H

D

D

T

F

L

T

L

K

R

T

M

T

K

V

L

E

E

Q

D

W

W

R

Q

Y

A

L

V

L

I

A

D

V

L

N

N

L

L

E

T

G

G

V

V

F

F

A

L

S

C

T

T

H

K

A

A

A

V

L

S

P

K

A

L

M

M

Q

L

A

K

A

Q

G

K

Y

S

G

G

R

R

I

I

N

N

V

I

G

T

S

S

E

V

A

A

G

G

R

M

Q

M

G

G

S

N

K
x
P

G

G

G

Q

A

A

V

N

Y

Y

A

S

A

A

K
|
K

G

A

G

G

V

V

I

I

A

G

F

F

S

T

K

K

S

T

I

V

A

A

R

K

E

E

N

L

A

A

R

S

Y

R

G

G

I

V

T

T

V

V

N

N

V

A

V

V

A

A

P

P

G

G

P
x
F

V

I

D

A

T

T

P

D

L

M

L

T

R

E

K

-

A

-

L

-

D

N

R

L

G
x
N

G

A

E

E

K

P

L

I

L

L

A

Q

A

F

M

I

E

P

A

-

S

-

T

-

L

-

V

L

G

A

R

R

L

Y

G

G

E

Q

P

P

N

E

E

E

V

V

A

A

A

G

A

T

V

I

V

R

F

F

L

L

A

A

S

T

R

D

D

P

-

A

A

A

S

A

F

Y

I

I

T

T

G

G

E

Q

T

T

L

F

G

N

V

V

S

D

G

G

M

M

A14 (≠ G16) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
P151 (≠ K143) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
K160 (= K152) mutation to A: Almost no activity on acetoacetyl-CoA.
F188 (≠ P180) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
N198 (≠ G193) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.

3tzcA Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg)(y155f) from vibrio cholerae (see paper)
41% identity, 97% coverage: 6:242/244 of query aligns to 6:223/224 of 3tzcA

query
sites
3tzcA

L

L

T

E

G

G

Q

K

R

V

A

A

F

L

V

V

T

T

G
|
G

G

A

S
|
S

K
x
R

G

G

I

I

G

G

E

K

A

A

I

I

V

A

R

E

R

L

L

L

A

A

A

E

E

R

G

G

A

A

K

K

V

V

V

I

I

G

A

T

A
|
A

Q
x
T

D

S

M

E

I

S

S

G

A

A

E

Q

R

A

L

I

A

S

D

D

E

Y

V

L

G

G

-

D

-

N

G

G

L

K

A

G

V

M

R

A

L

L

D
x
N

V
|
V

T

T

D

N

L

P

L

E

E

S

V

I

E

E

R

A

V

V

V

L

T

K

M

A

L

I

-

T

-

D

-

E

-

F

G

G

P

G

F

V

E

D

I

I

L

L

V

V

N

N

A

A

G
|
G

Y
x
I

D

T

Q

R

H

M

D

-

F

-

T

-

K

R

T

M

T

K

V

E

E

E

Q

E

W

W

R

S

Y

D

L

I

L

M

A

E

V
x
T

N

N

L

L

E

T

G

S

V

I

F

F

A

R

S

L

T

S

H

K

A

A

A

V

L

L

P

R

A

G

M

M

Q

M

A

K

G

R

Y

Q

G

G

R

R

I

I

N

N

V

V

G

G

S

S

E

-

A

-

G

-

R

-

Q

-

G

-

S

-

K

-

G

-

A

-

V

-

Y

V

A

V

A

A

K

K

G

A

G

G

V

V

I

I

A

G

F

F

S

T

K

K

S

S

I

M

A

A

R

R

E

E

N

V

A

A

R

S

Y

R

G

G

I

V

T

T

V

V

N

N

V

T

V

V

A

A

P

P

G

G

P

F

V

I

D

E

T

T

P

D

L

M

L

-

R

-

K

-

A

-

L

-

D

-

R

-

G

-

E

D

K

E

L

Q

L

R

A

T

A

A

M

T

E

L

A

A

S

Q

T

V

L

P

V

A

G

G

R

R

L

L

G

G

E

D

P

P

N

R

E

E

V

I

A

A

A

S

A

A

V

V

V

A

F

F

L

L

A

A

S

S

R

P

D

E

A

A

S

A

F

Y

I

I

T

T

G

G

E

E

T

T

L

L

G

H

V

V

S

N

G

G

M

M

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G15), S17 (= S17), R18 (≠ K18), A39 (= A39), T40 (≠ Q40), N62 (≠ D60), V63 (= V61), G91 (= G85), I92 (≠ Y86), T108 (≠ V106)

Query Sequence

>Pf6N2E2_1839 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1839
MTMRALTGQRAFVTGGSKGIGEAIVRRLAAEGAKVVIAAQDMISAERLADEVGGLAVRLD
VTDLLEVERVVTMLGPFEILVNNAGYDQHDFFTKTTVEQWRYLLAVNLEGVFASTHAALP
AMQAAGYGRIINVGSEAGRQGSKGGAVYAAAKGGVIAFSKSIARENARYGITVNVVAPGP
VDTPLLRKALDRGGEKLLAAMEASTLVGRLGEPNEVAAAVVFLASRDASFITGETLGVSG
GMGC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory