SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_1863 FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1863 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7ylsB Structure of a bacteria protein complex
31% identity, 89% coverage: 13:383/419 of query aligns to 13:400/436 of 7ylsB

query
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7ylsB

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binding fe (iii) ion: H215 (= H210), H220 (= H215), D376 (= D359)
binding fe2/s2 (inorganic) cluster: C84 (= C84), H86 (= H86), K87 (≠ R87), S89 (≠ N89), C105 (= C104), H108 (= H107), W110 (= W109)

8h2tB Cryo-em structure of iadd/e dioxygenase bound with iaa
31% identity, 89% coverage: 13:383/419 of query aligns to 12:399/435 of 8h2tB

query
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8h2tB

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binding fe (iii) ion: N208 (= N204), H214 (= H210), H219 (= H215), D375 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), K86 (≠ R87), C104 (= C104), H107 (= H107), W109 (= W109)
binding 1h-indol-3-ylacetic acid: N208 (= N204), L209 (≠ E205), D211 (= D207), H214 (= H210), P215 (= P211), F249 (vs. gap), K320 (vs. gap), Y360 (≠ Q344)

O85673 Anthranilate 1,2-dioxygenase large subunit; EC 1.14.12.1 from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) (see 2 papers)
32% identity, 87% coverage: 18:383/419 of query aligns to 27:403/471 of O85673

query
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O85673

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M43 (≠ L34) mutation to K: Prevents anthranilate degradation.
D217 (= D207) mutation to A: In ACN476; loss of dioxygenase activity and 2-fold lower redox potential.; mutation to E: Loss of dioxygenase activity and lack of iron at the mononuclear site.; mutation to N: Loss of dioxygenase activity.

2xrxA Crystal structure of biphenyl dioxygenase in complex with biphenyl from burkholderia xenovorans lb400 (see paper)
29% identity, 94% coverage: 23:416/419 of query aligns to 22:422/432 of 2xrxA

query
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2xrxA

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T

N

G

D

D

F

F

V

L

V

A

K

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I

M

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G

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V

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I

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L

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N

N

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|
C

P

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|
H

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|
R

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M

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C
|
C

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Y
|
Y

H
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H

G

G

W
|
W

T

A

F

Y

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N

I

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A

G

G

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L

L

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N

Y

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W

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F

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Q

F
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F

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|
D

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M

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H

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|
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P

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C

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F

F

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x
L

A

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x
F

Q

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I

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x
F

L

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D

D
|
D

S

G

E

E

M

N

Y

W

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V

R

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T

I

Q

Q

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K

G

G

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L

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G

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Y

N

K

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A

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K

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L

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N

-

A

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Q

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M

G

G

L

L

A

G

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S

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Q

R

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D

G

A

H

D

P

G

D

F

F

L

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-

G

-

N

I

V

G

G

D

Y

A

V

T

Y

D

A

E

E

V

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T

A

T

A

R

R

G

G

I

M

W

Y

S

H

H

H

Y

W

R

M

S

R

L

M

M

M

active site: H106 (= H107), D203 (= D220), H206 (≠ S223), H212 (≠ L229), D361 (= D359)
binding biphenyl: Q199 (= Q212), F200 (= F213), D203 (= D220), H206 (≠ S223), H296 (= H297), L306 (≠ I306), F309 (≠ I309), F357 (≠ L355)
binding fe (ii) ion: Q199 (= Q212), H206 (≠ S223), H212 (≠ L229), D361 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), R86 (= R87), C103 (= C104), Y105 (= Y106), H106 (= H107), W108 (= W109)

2yflA Crystal structure of biphenyl dioxygenase variant rr41 with 2-chloro dibenzofuran (see paper)
29% identity, 94% coverage: 23:416/419 of query aligns to 22:423/433 of 2yflA

query
sites
2yflA

V

I

Y

Y

T

A

D

D

E

Q

E

S

L

L

F

Y

Q

E

L

L

E

E

L

L

E

E

R

R

I

V

W

F

Y

G

R

R

T

S

W

W

V

L

Y

L

V

L

G

G

H

H

V

E

S

S

E

H

V

V

P

P

K

E

L

T

N

G

D

D

F

F

V

L

V

A

K

T

S

Y

I

M

G

G

P

E

Q

D

P

P

V

V

I

V

M

M

T

V

R

R

D

Q

G

K

E

D

G

K

Q

S

I

I

H

K

L

V

L

F

L

L

N

N

R

Q

C
|
C

P

R

H
|
H

R
|
R

G

G

N

M

Q

R

V

I

C

C

L

R

E

S

H

D

A

A

G

G

N

N

R

A

R

K

S

A

F

F

T

T

C
|
C

P

S

Y
|
Y

H
|
H

G

G

W
|
W

T

A

F

Y

S

D

N

I

T

A

G

G

T

K

L

L

R

V

G

N

Y

V

P

P

F

F

A

E

-

K

-

E

A

A

G

F

Y

F

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D

G

K

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D

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S

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T

P

L

L

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L

-

G

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K

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Q

A

A

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R

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V

G

A

I

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Y

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K

G

G

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L

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V

F

F

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N

M

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A

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V

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Q

G

A

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P

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D

L

L

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E

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Y

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L

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G

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A

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D

D

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L

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L

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P

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A

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G

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A

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L

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Q

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A

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N

N

W

W

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K

F

F

I

A

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A

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E

N

-

E

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D

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G

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Y

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H

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Q
|
Q

F

F

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C

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A

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S

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I

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F

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D
|
D

V
x
M

A

Y

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x
H

S

A

G

G

I

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G

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T

L
x
H

Y

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D

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|
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L

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V

L

F

L

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D

D

D
|
D

S

G

E

E

M

N

Y

W

E

V

R

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T

I

Q

Q

R

K

G

G

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L

R

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G

R

Y

N

K

P

A

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K

W

S

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Q

Y

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F

G

N

-

A

-

Q

R

M

G

G

L

L

A

G

R

R

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S

R

Q

R

T

D

G

A

H

D

P

G

D

F

F

L

P

-

G

-

N

I

V

G

G

D

Y

A

V

T

Y

D

A

E

E

V

E

T

A

T

A

R

R

G

G

I

M

W

Y

S

H

H

H

Y

W

R

M

S

R

L

M

M

M

active site: H106 (= H107), D204 (= D220), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding 2-chlorodibenzofuran: Q200 (= Q212), D204 (= D220), M205 (≠ V221), H207 (≠ S223), S257 (≠ R270), H297 (= H297), L307 (≠ F305), F352 (≠ V349)
binding fe (ii) ion: Q200 (= Q212), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), R86 (= R87), C103 (= C104), Y105 (= Y106), H106 (= H107), W108 (= W109)

2yfjA Crystal structure of biphenyl dioxygenase variant rr41 with dibenzofuran (see paper)
29% identity, 94% coverage: 23:416/419 of query aligns to 22:423/433 of 2yfjA

query
sites
2yfjA

V

I

Y

Y

T

A

D

D

E

Q

E

S

L

L

F

Y

Q

E

L

L

E

E

L

L

E

E

R

R

I

V

W

F

Y

G

R

R

T

S

W

W

V

L

Y

L

V

L

G

G

H

H

V

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S

S

E

H

V

V

P

P

K

E

L

T

N

G

D

D

F

F

V

L

V

A

K

T

S

Y

I

M

G

G

P

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Q

D

P

P

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V

I

V

M

M

T

V

R

R

D

Q

G

K

E

D

G

K

Q

S

I

I

H

K

L

V

L

F

L

L

N

N

R

Q

C
|
C

P

R

H
|
H

R
|
R

G

G

N

M

Q

R

V

I

C

C

L

R

E

S

H

D

A

A

G

G

N

N

R

A

R

K

S

A

F

F

T

T

C
|
C

P

S

Y
|
Y

H
|
H

G

G

W
|
W

T

A

F

Y

S

D

N

I

T

A

G

G

T

K

L

L

R

V

G

N

Y

V

P

P

F

F

A

E

-

K

-

E

A

A

G

F

Y

F

E

D

G

K

T

A

D

E

R

W

S

G

T

P

L

L

G

-

L

-

G

-

K

-

V

Q

A

A

R

R

I

V

G

A

I

T

Y

Y

Q

K

G

G

F

L

V

V

F

F

G

A

S

N

M

W

A

D

V

V

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Q

G

A

P

P

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D

L

L

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E

E

T

H

Y

L

L

G

G

A

D

A

A

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R

G

P

A

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L

M

D

D

A

V

L

M

V

L

V

D

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R

S

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P

P

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A

G

G

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V

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A

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I

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-

A

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G

G

F

M

L

Q

Q

K

H

W

K

V

A

I

K

P

S

C

N

N

W

W

K

K

F

F

I

A

V

A

E

E

N

-

E

-

T

-

D

-

G

-

Y

-

H

-

P

-

Q
|
Q

F
|
F

V

C

H

S

A

-

S

-

I

-

F

-

D
|
D

V
x
M

A

Y

S
x
H

S
x
A

G

G

I

T

G

T

D

T

L
x
H

Y

L

S

S

A

G

D

I

S

L

T

A

A

G

V

I

A

P

R

P

D

E

M

M

G

D

G

L

G

S

H

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T

A

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Q

I

I

D

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L

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R

K

-

G

P

N

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F

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R

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A

I

A

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W

-

G

G

G

Q

H

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M

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W

W

F

Y

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D

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L

L

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L

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A

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M

-

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P

P

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K

Y

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Q

K

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W

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A

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P

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A

A

N

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M

L

-

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I

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D

T

G

G

T

M

P

P

-

V

-

R

-

R

-

M

-

V

-

G

-

Q

H
|
H

I

M

M

T

I

I

F

F

P

P

-

T

-

C

N

S

L

F

F
x
L

I

P

A

A

E

M

I

N

Q

Q

I

I

F

R

V

V

I

W

Q

H

P

P

L

R

A

G

V

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I

-

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V

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W

H

A

V

F

T

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A

L

L

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R

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R

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T

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M

I

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R

S

N

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F

G

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P

A

A

G

G

G

F

V

L
x
F

L

E

A

Q

D

D

D
|
D

S

G

E

E

M

N

Y

W

E

V

R

E

T

I

Q

Q

R

K

G

G

V

L

R

R

S

G

R

Y

N

K

P

A

E

K

W

S

N

Q

Y

P

L

F

G

N

-

A

-

Q

R

M

G

G

L

L

A

G

R

R

E

S

R

Q

R

T

D

G

A

H

D

P

G

D

F

F

L

P

-

G

-

N

I

V

G

G

D

Y

A

V

T

Y

D

A

E

E

V

E

T

A

T

A

R

R

G

G

I

M

W

Y

S

H

H

H

Y

W

R

M

S

R

L

M

M

M

active site: H106 (= H107), D204 (= D220), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding dibenzofuran: Q200 (= Q212), F201 (= F213), D204 (= D220), M205 (≠ V221), H207 (≠ S223), A208 (≠ S224), H297 (= H297), L307 (≠ F305), F358 (≠ L355)
binding fe (ii) ion: Q200 (= Q212), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), R86 (= R87), C103 (= C104), Y105 (= Y106), H106 (= H107), W108 (= W109)

2xshA Crystal structure of p4 variant of biphenyl dioxygenase from burkholderia xenovorans lb400 in complex with 2,6 di chlorobiphenyl (see paper)
28% identity, 94% coverage: 23:416/419 of query aligns to 22:423/433 of 2xshA

query
sites
2xshA

V

I

Y

Y

T

A

D

D

E

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S

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Y

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E

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-

K

-

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L

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D

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x
M

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|
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F

L

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|
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W

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Q

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-

A

-

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L

L

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Q

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D

G

A

H

D

P

G

D

F

F

L

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-

G

-

N

I

V

G

G

D

Y

A

V

T

Y

D

A

E

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V

E

T

A

T

A

R

R

G

G

I

M

W

Y

S

H

H

H

Y

W

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S

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M

M

active site: H106 (= H107), D204 (= D220), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding 2,6-dichlorobiphenyl: F201 (= F213), M205 (≠ V221), H207 (≠ S223), Q296 (vs. gap), H297 (= H297), L307 (≠ F305), F358 (≠ L355)
binding fe (ii) ion: Q200 (= Q212), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), R86 (= R87), C103 (= C104), Y105 (= Y106), H106 (= H107), W108 (= W109)

1uliC Biphenyl dioxygenase (bpha1a2) derived from rhodococcus sp. Strain rha1 (see paper)
29% identity, 95% coverage: 18:416/419 of query aligns to 16:411/425 of 1uliC

query
sites
1uliC

R

R

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M

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G

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N

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|
C

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H

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R

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G

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M

Q

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C

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A

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A

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G

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N

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C
|
C

P

S

Y
|
Y

H
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H

G

G

W
|
W

T

A

F

Y

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D

N

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G

G

G

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L

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|
D

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M

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x
H

A

A

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H

A

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F

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N

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L

A

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E

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M

G

G

L

L

A

G

R

Q

E

T

R

D

R

S

D

D

A

N

D

P

G

D

F

Y

-

P

-

G

L

T

I

I

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Y

A

V

T

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D

S

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E

V

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T

A

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A

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M

active site: H105 (= H107), D205 (= D228), H208 (≠ S231), H214 (≠ V237), D350 (= D359)
binding fe (ii) ion: H208 (≠ S231), H214 (≠ V237), D350 (= D359)
binding fe2/s2 (inorganic) cluster: C82 (= C84), H84 (= H86), R85 (= R87), C102 (= C104), Y104 (= Y106), H105 (= H107), W107 (= W109)

5aeuA Crystal structure of ii9 variant of biphenyl dioxygenase from burkholderia xenovorans lb400 (see paper)
29% identity, 94% coverage: 23:416/419 of query aligns to 22:423/433 of 5aeuA

query
sites
5aeuA

V

I

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Y

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D

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|
C

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H

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R

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x
M

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A

A

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|
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x
H

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W

S

N

Q

Y

P

L

L

G

N

-

A

-

Q

R

M

G

G

L

L

A

G

R

R

E

S

R

Q

R

T

D

G

A

H

D

P

G

D

F

F

L

P

-

G

-

N

I

V

G

G

D

Y

A

V

T

Y

D

A

E

E

V

E

T

A

T

A

R

R

G

G

I

M

W

Y

S

H

H

H

Y

W

R

M

S

R

L

M

M

M

active site: H106 (= H107), D204 (= D220), H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding fe (ii) ion: H207 (≠ S223), H213 (≠ L229), D362 (= D359)
binding fe2/s2 (inorganic) cluster: C83 (= C84), H85 (= H86), R86 (= R87), M88 (≠ N89), C103 (= C104), Y105 (= Y106), H106 (= H107), W108 (= W109)

1uljA Biphenyl dioxygenase (bpha1a2) in complex with the substrate (see paper)
28% identity, 95% coverage: 18:416/419 of query aligns to 16:413/425 of 1uljA

query
sites
1uljA

R

R

V

L

H

D

G

P

S

R

V

I

Y

Y

T

T

D

D

E

E

E

A

L

L

F

Y

Q

E

L

Q

E

E

L

L

E

E

R

R

I

I

W

F

Y

G

R

R

T

S

W

W

V

L

Y

L

V

M

G

G

H

H

V

E

S

T

E

Q

V

I

P

P

K

K

L

A

N

G

D

D

F

F

V

M

V

T

K

N

S

Y

I

M

G

G

P

E

Q

D

P

P

V

V

I

M

M

V

T

V

R

R

D

Q

G

K

E

N

G

G

Q

E

I

I

H

R

L

V

L

F

L

L

N

N

R

Q

C
|
C

P

R

H
|
H

R
|
R

G

G

N
x
M

Q

R

V

I

C

C

L

R

E

A

H

D

A

G

G

G

N

N

R

A

R

K

S

S

F

F

T

T

C
|
C

P

S

Y
|
Y

H
|
H

G

G

W
|
W

T

A

F

Y

S

D

N

T

T

G

G

G

T

N

L

L

R

V

G

S

Y

V

P

P

F

F

-

E

A

E

A

Q

G

A

Y

F

E

P

G

G

T

L

D

R

R

K

S

E

T

D

L

W

G

G

L

P

G

L

K

Q

V

-

A

A

R

R

I

V

G

E

I

T

Y

Y

Q

K

G

G

F

L

V

I

F

F

G

A

S

N

M

W

A

D

V

A

E

D

G

A

P

P

T

D

L

L

E

D

E

T

H

Y

L

L

G

G

A

E

A

A

R

K

G

F

A

Y

L

M

D

D

A

H

L

M

V

L

V

D

T

R

S

T

P

E

E

A

G

G

E

-

V

T

E

E

I

A

T

I

A

P

G

G

F

I

L

Q

Q

K

H

W

K

V

A

I

K

P

S

C

N

N

W

W

K

K

F

F

I

A

V

A

E

E

N
x
Q

E

-

T

-

D

-

G

-

Y
x
F

H

C

P

S

Q
x
D

F
x
M

V

Y

H
|
H

A
|
A

S

G

I

T

F

T

D

S

V
x
H

A

L

S

S

S

G

G

I

I

L

G

A

D

G

L

L

Y

P

S

P

A

T

D

E

S

G

T

I

A

Q

V

Y

A

R

R

A

D

T

M

W

G

G

G

G

G

H

H

G

T

S

E

G

I

F

D

-

L

-

R

-

P

-

E

-

F

-

R

-

R

Y

I

I

G

G

Q

D

P

P

M

N

G

L

W

L

F

L

G

A

T
x
I

T

M

E

G

A

P

R

K

L

V

P

T

E

E

Y

Y

-

W

-

T

-

Q

-

G

-

P

-

A

V

A

S

E

K

K

M

A

R

S

E

E

A

R

Y

L

G

G

-

S

T

T

E

E

R

R

A

G

N

Q

E

Q

I

L

M

M

I

A

D

Q

G

-

T

-

P

-

H
|
H

I

M

M

T

I

I

F

F

P

P

N

T

L

C

-

S

F

F

I
x
L

A

P

E

G

I

I

Q

N

-

T

I

I

F

R

V

A

I

W

Q

H

P

P

L

R

A

G

V

P

D

N

E

E

T

I

-

E

V

V

Q

W

H

A

V

F

T

T

A

V

L

V

Q

D

F

A

K

D

G

A

A

P

P

E

D

E

L

M

N

K

R

E

R

E

L

Y

R

R

Q

Q

Q

Q

T

T

M

L

G

R

S

T

V
x
F

G

S

P

A

A

G

G

G

F

V

L

F

L

E

A

Q

D

D

D
|
D

S

G

E

E

M

N

Y

W

E

V

R

E

T

I

Q

Q

-

Q

-

V

-

L

R

R

G

G

V

H

R

K

S

A

R

R

N

S

P

R

E

P

W

F

N

N

Y

-

L

A

G

E

R

M

G

G

L

L

A

G

R

Q

E

T

R

D

R

S

D

D

A

N

D

P

G

D

F

Y

-

P

-

G

L

T

I

I

G

S

D

Y

A

V

T

Y

D

S

E

E

V

E

T

A

T

A

R

R

G

G

I

L

W

Y

S

T

H

Q

Y

W

R

V

S

R

L

M

M

M

active site: H105 (= H107), D205 (≠ Q212), H208 (= H215), H214 (≠ V221), D352 (= D359)
binding biphenyl: Q201 (≠ N204), F202 (≠ Y209), D205 (≠ Q212), M206 (≠ F213), H208 (= H215), A209 (= A216), H214 (≠ V221), I252 (≠ T266), H287 (= H297), L297 (≠ I306), F342 (≠ V349)
binding fe (ii) ion: Q201 (≠ N204), H208 (= H215), H214 (≠ V221), D352 (= D359)
binding fe2/s2 (inorganic) cluster: C82 (= C84), H84 (= H86), R85 (= R87), M87 (≠ N89), C102 (= C104), Y104 (= Y106), H105 (= H107), W107 (= W109)

4hm8A Naphthalene 1,2-dioxygenase bound to thioanisole
29% identity, 96% coverage: 13:414/419 of query aligns to 14:422/446 of 4hm8A

query
sites
4hm8A

L

L

V

S

Q

Q

H

K

D

H

R

L

V

I

H

H

G

G

S

-

V

-

Y

-

T

-

D

D

E

E

E

E

L

L

F

F

Q

Q

L

H

E

E

L

L

E

K

R

T

I

I

W

F

Y

A

R

R

T

N

W

W

V

L

Y

F

V

L

G

T

H

H

V

D

S

S

E

L

V

I

P

P

K

A

L

P

N

G

D

D

F

Y

V

V

V

T

K

A

S

K

I

M

G

G

P

I

Q

D

P

E

V

V

I

I

M

V

T

S

R

R

D

Q

G

N

E

D

G

G

Q

S

I

I

H

R

L

A

L

F

L

L

N

N

R

V

C
|
C

P

R

H
|
H

R
|
R

G

G

N

K

Q

T

V

L

C

V

L

S

E

V

H

E

A

A

G

G

N

N

R

A

R

K

S

G

F

F

T

V

C
|
C

P

S

Y
|
Y

H
|
H

G

G

W
|
W

T

G

F

F

S

G

N

S

T

N

G

G

T

E

L

L

R

Q

G

S

Y

V

P

P

F

F

A

E

A

K

G

D

Y

L

-

Y

-

G

E

E

G

S

T

L

D

N

R

K

S

K

T

C

L

L

G

G

L

L

G

K

K

E

V

V

A

A

R

R

I

V

G

E

I

S

Y

F

Q

H

G

G

F

F

V

I

F

Y

G

G

S

C

M

F

A

D

V

Q

E

E

G

A

P

P

T

P

L

L

E

M

E

D

H

Y

L

L

G

G

A

D

A

A

R

A

G

W

A

Y

L

L

D

E

A

P

L

M

V

F

V

K

T

H

S

S

P

-

E

-

G

G

E

G

V

L

E

E

I

L

T

V

A

G

G

P

F

P

L

G

Q

K

H

V

K

V

A

I

K

K

S

A

N

N

W

W

K

K

F

A

I

P

V

A

E

E

N
|
N

E

F

T

V

-

G

D
|
D

G

A

Y

Y

H
|
H

P

V

Q

G

F

W

V

T

H
|
H

A

A

S

S

I

S

F

L

D

R

V

S

A

G

S

E

S

S

G

I

I

F

G

S

D

S

L

L

Y

A

S

G

-

N

-

A

-

A

-

L

-

P

-

P

-

E

-

G

-

A

-

G

-

L

-

Q

-

M

-

T

-

S

-

K

-

Y

A

G

D

S

S

G

T

M

A

G

V

V

A

L

R

W

D

D

M

G

G

Y

G

S

G

G

H

V

T

H

E

S

I

A

D

D

L

L

R

V

P

P

E

E

F

L

R

M

R

A

I

F

G

G

Q

-

P

-

M

-

G

-

W

-

F

-

G

G

T

A

T

K

E

Q

A

E

R

R

L

L

P

N

E

K

Y

E

V

I

S

G

K

D

M

V

R

R

E

A

A

R

Y

I

G

Y

T

R

E

S

R
x
H

A

L

N

N

E

-

I

-

M

-

I

-

D

-

G

-

T

-

P

-

H

-

I

C

M

T

I

V

F

F

P

P

N

N

-

N

-

S

L

M

F

L

I

T

A

C

E

S

I

G

Q

V

I

F

F

K

V

V

I

W

Q

N

P

P

L

I

A

D

V

A

D

N

E

T

T

T

V

E

Q

V

H

W

V

T

T

Y

A

A

L

I

Q

V

F

E

K

K

G

D

A

M

P

P

-

E

D

D

L

L

N

K

R

R

R

R

L

L

R

A

Q

D

Q

S

T

V

M

Q

G

R

S

T

V

F

G

G

P

P

A

A

G

G

F

F

L

W

L

E

A

S

D

D

D
|
D

S

N

E

D

-

N

M

M

Y

E