SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Pf6N2E2_1903 Putative diheme cytochrome c-553 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q47945 Alcohol dehydrogenase (quinone), cytochrome c subunit; ADH; Alcohol dehydrogenase (quinone), subunit II; Cytochrome c-553; Cytochrome c553; Ethanol:Q2 reductase; G3-ADH subunit II; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
47% identity, 91% coverage: 35:436/444 of query aligns to 34:442/478 of Q47945

query
sites
Q47945

A
|
A

S
x
H

A
|
A

P
x
Q

S

D

A

A

D

D

A

E

Q

A

L

L

V

I

Q

K

R

R

G

G

E

E

Y

Y

V

V

A

A

R

R

L

L

S

S

D

D

C

C

V

I

A

A

C

C

H

H

S

T

T

A

P

L

K

H

G

G

A

Q

P

P

F

Y

A

A

G

G

L

L

E

E

M

I

A

K

T

S

P

P

M

I

G

G

S

T

I

I

Y

Y

A

S

T

T

N

N

I

I

T

T

P

P

D

D

K

P

Q

E

T

H

G

G

I

I

G

G

N

N

Y

Y

S

T

L

L

A

E

D

D

F

F

D

T

R

K

A

A

V

L

R

R

S

K

G

G

V

I

A

R

A

K

D

D

G

G

H

A

R

T

L

V

Y

Y

P

P

A

A

M

M

P

P

Y

Y

P

P

S

E

Y

F

A

A

K

R

L

L

S

S

D

D

V

I

R

R

A

A

L

M

Y

Y

A

A

F

F

M

M

A

H

G

G

V

V

K

K

P

P

A

V

Q

A

Q

L

Q

Q

N

N

Q

K

Q

A

S

P

H

D

I

I

P

S

W

W

P

P

L

L

N

S

M

M

R

R

W

W

P

P

L

L

A

G

L

M

W

W

N

R

T

A

A

M

F

F

V

V

D

P

D

S

G

M

A

T

Y

P

Q

G

A

V

K

D

P

K

S

S

-

I

E

S

D

D

A

P

L

E

W

V

N

A

R

R

G

G

A

E

Y

Y

I

L

V

V

Q

N

G

G

A

P

G

G

H

H

C

C

G

G

S

E

C

C

H

H

T

T

P

P

R

R

S

G

L

F

T

G

M

M

N

Q

E

V

K

K

S

A

L

Y

D

G

E

T

S

A

-

G

S

N

A

A

T

Y

F

L

L

A

S

G

G

G

S

A

L

P

L

I

D

D

G

N

W

W

Y

I

A

A

P

P

S

S

L

L

R

R

Q

S

D

N

P

S

N

D

T

T

G

G

L

L

G

G

R

R

W

W

S

S

E

E

Q

D

E

D

I

I

V

V

D

T

Y

F

L

L

K

K

T

S

G

G

R

R

N

I

A

D

H

H

S

S

V

A

V

V

V

F

G

G

T

G

M

M

A

A

E

D

V

V

F

V

N

A

N

Y

S

S

T

T

Q

Q

Y

H

M

W

S

S

D

D

P

D

D

D

L

L

K

R

A

A

I

T

A

A

H

K

Y

Y

L

L

V

K

S

S

L

M

P

P

G

A

D

V

P

P

-

E

-

G

-

K

-

N

-

L

-

G

K

Q

R

D

D

D

G

G

P

-

W

-

K

Q

P

T

A

T

A

A

K

L

L

L

A

N

E

K

Q

G

P

G

L

Q

S

G

T

N

P

A

G

G

A

A

A

E

N

V

Y

Y

M

L

A

H

K

N

C

C

S

A

S

I

C

C

H

H

G

M

S

N

D

D

G

G

S

T

G

G

Q

V

A

N

P

R

W

M

I

F

P

P

L

L

A

A

G

G

A

N

S

P

S

V

M

I

V

T

K

D

E

D

G

P

A

T

T

S

S

L

I

A

N

N

A

V

T

V

L

A

N

F

G

G

S

G

E

I

R

L

V

P

A

T

N

N

G

S

I

A

P

P

D

S

S

A

Y

V

R

A

M

M

P

P

P

G

Y

F

R

K

N

N

Q

H

L

L

S

S

D

D

Q

Q

E

E

V

M

A

A

D

D

V

V

L

V

T

N

F

F

V

M

R

R

T

K

S

G

W

W

G

G

N

N

Q

N

G

A

-

P

G

G

A

T

V

V

K

S

A

A

D

S

E

D

V

I

K

Q

E

K

L

L

R

R

E

T

R

T

T

G

N

A

P

P

A

V

S

S

S

T

Q37 (≠ P38) modified: Pyrrolidone carboxylic acid

Sites not aligning to the query:

1:36 signal peptide

8gy2B Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
47% identity, 89% coverage: 40:436/444 of query aligns to 1:404/433 of 8gy2B

query
sites
8gy2B

A

A

D

D

A

E

Q

A

L

L

V

I

Q

K

R

R

G

G

E

E

Y

Y

V

V

A

A

R

R

L

L

S

S

D

D

C
|
C

V

I

A

A

C
|
C

H
|
H

S

T

T

A

P

L

K

H

G

G

A

Q

P

P

F

Y

A

A

G

G

L
|
L

E

E

M

I

A

K

T

S

P
|
P

M

I

G

G

S

T

I

I

Y

Y

A

S

T
|
T

N

N

I
|
I

T

T

P

P

D

D

K

P

Q

E

T

H

G

G

I

I

G

G

N

N

Y
|
Y

S

T

L

L

A

E

D

D

F

F

D

T

R

K

A

A

V
x
L

R

R

S

K

G

G

V

I

A
x
R

A

K

D

D

G

G

H

A

R

T

L
x
V

Y
|
Y

P
|
P

A

A

M
|
M

P

P

Y

Y

P

P

S

E

Y
x
F

A

A

K

R

L

L

S

S

D

D

V

I

R

R

A

A

L

M

Y

Y

A

A

F

F

M

M

A

H

G

G

V

V

K

K

P

P

A

V

Q

A

Q

L

Q

Q

N

N

Q

K

Q

A

S

P

H

D

I

I

P

S

W

W

P

P

L

L

N

S

M

M

R
|
R

W

W

P

P

L
|
L

A

G

L

M

W
|
W

N

R

T

A

A

M

F

F

V

V

D

P

D

S

G

M

A

T

Y

P

Q

G

A

V

K

D

P

K

S

S

-

I

E

S

D

D

A

P

L

E

W

V

N

A

R

R

G

G

A

E

Y

Y

I

L

V

V

Q

N

G

G

A

P

G

G

H
|
H

C
|
C

G

G

S
x
E

C
|
C

H
|
H

T

T

P

P

R
|
R

S

G

L

F

T

G

M

M

N

Q

E

V

K

K

S

A

L

Y

D

G

E

T

S

A

-

G

S

N

A

A

T

Y

F

L

L

A

S

G

G

G

S

A

L

P

L
x
I

D

D

G

N

W

W

Y
x
I

A
|
A

P
|
P

S

S

L
|
L

R

R

Q

S

D

N

P

S

N

D

T

T

G

G

L

L

G

G

R

R

W
|
W

S

S

E

E

Q

D

E

D

I

I

V

V

D

T

Y
x
F

L

L

K

K

T

S

G

G

R

R

N

I

A

D

H

H

S

S

V
x
A

V
|
V

V
x
F

G

G

T

G

M
|
M

A

A

E
x
D

V

V

F

V

N

A

N

Y

S

S

T

T

Q

Q

Y

H

M

W

S

S

D

D

P

D

D

D

L

L

K

R

A

A

I

T

A

A

H

K

Y

Y

L

L

V

K

S

S

L

M

P

P

G

A

D

V

P

P

-

E

-

G

-

K

-

N

-

L

-

G

K

Q

R

D

D

D

G

G

P

-

W

-

K

Q

P

T

A

T

A

A

K

L

L

L

A

N

E

K

Q

G

P

G

L

Q

S

G

T

N

P

A

G

G

A

A

A

E

N

V

Y

Y

M

L

A

H

K
x
N

C
|
C

S

A

S

I

C
|
C

H
|
H

G

M

S

N

D

D

G

G

S

T

G

G

Q

V

A

N

P

R

W
x
M

I
x
F

P
|
P

P

P

L
|
L

A

A

G

G

A

N

S
x
P

S

V

M

I

V

T

K

D

E

D

G

P

A

T

T

S

S

L

I

A

N

N

A

V

T

V

L

A

N

F

G

G

S
x
G

E

I

R

L

V

P

A

T

N

N

G

S

I

A

P

P

D
x
S

S

A

Y
x
V

R

A

M
|
M

P

P

P

G

Y
x
F

R

K

N

N

Q

H

L

L

S

S

D

D

Q

Q

E

E

V

M

A

A

D

D

V

V

L

V

T

N

F

F

V
x
M

R

R

T

K

S

G

W

W

G

G

N

N

Q

N

G

A

-

P

G

G

A

T

V

V

K

S

A

A

D

S

E

D

V

I

K

Q

E

K

L

L

R

R

E

T

R

T

T

G

N

A

P

P

A

V

S

S

S

T

binding heme c: C18 (= C57), C21 (= C60), H22 (= H61), T46 (= T85), I48 (= I87), Y59 (= Y98), L68 (≠ V107), R73 (≠ A112), V79 (≠ L118), Y80 (= Y119), M83 (= M122), F88 (≠ Y127), R126 (= R165), H165 (= H203), C166 (= C204), C169 (= C207), H170 (= H208), I201 (≠ Y237), A202 (= A238), P203 (= P239), L205 (= L241), W216 (= W252), F224 (≠ Y260), A234 (≠ V270), V235 (= V271), F236 (≠ V272), F236 (≠ V272), M239 (= M275), N301 (≠ K334), C302 (= C335), C305 (= C338), H306 (= H339), M316 (≠ W349), F317 (≠ I350), P318 (= P351), L320 (= L353), P324 (≠ S357), G342 (≠ S375), S352 (≠ D385), V354 (≠ Y387), M356 (= M389), F359 (≠ Y392), M375 (≠ V408)
binding ubiquinone-10: C21 (= C60), L34 (= L73), P39 (= P78), P81 (= P120), L129 (= L168), W132 (= W171), E168 (≠ S206), R173 (= R211), I197 (≠ L233), D241 (≠ E277)

Sites not aligning to the query:

binding heme c: 428

7w2jC Cryo-em structure of membrane-bound fructose dehydrogenase from gluconobacter japonicus
38% identity, 86% coverage: 45:428/444 of query aligns to 1:386/418 of 7w2jC

query
sites
7w2jC

V

I

Q

S

R

R

G

G

E

H

Y

Y

V

L

A

A

R

I

L

A

S

A

D

D

C
|
C

V

A

A

A

C
|
C

H
|
H

S

T

T

N

P

G

K

R

G

D

A

G

P

Q

F

F

-

L

A

A

G

G

L

Y

E

A

M

I

A

S

T

S

P

P

M

M

G

G

S

N

I

I

Y

Y

A

S

T
|
T

N

N

I
|
I

T

T

P

P

D

S

K

K

Q

T

T

H

G

G

I

I

G

G

N

N

Y
|
Y

S

T

L

L

A

E

D

Q

F

F

D

S

R

K

A

A

V

L

R

R

S

H

G

G

V

I

A

R

A

A

D

D

G

G

H

A

R

Q

L
|
L

Y
|
Y

P

P

A
|
A

M
|
M

P

P

Y

Y

P

D

S

A

Y

Y

A

N

K

R

L

L

S

T

D

D

D

E

D

D

V

V

R

K

A

S

L

L

Y

Y

A

A

F

Y

F

I

M

M

A

T

G

E

V

V

K

K

P

P

A

V

Q

D

Q

A

Q

P

N

S

Q

P

Q

K

S

T

H

Q

I

L

P

P

W

F

P

P

L

F

N

S

M

I

R
|
R

W

A

P

S

L

L

A

G

L

I

W

W

N

K

T

I

A

A

F

A

V

R

D

I

D

E

G

G

A

K

-

P

Y

Y

Q

V

A

F

K

D

P

H

S

T

E

H

D

N

A

D

L

D

W

W

N

N

R

R

G

G

A

R

Y

Y

I

L

V

V

Q

D

G

E

A

L

G

A

H
|
H

C
|
C

G

G

S

E

C
|
C

H
|
H

T

T

P

P

R

R

S

N

L

F

T

L

M

-

N

-

E

-

K

-

S

-

L

L

D

A

E

P

S

N

S

Q

A

S

T

A

F

Y

L

L

S

A

G

G

S

A

L

D

L

I

D

G

G

S

W

W

Y

R

A
|
A

P
|
P

S

N

L

I

R

T

Q

N

D

A

P

P

N

Q

T

S

G

G

L

I

G

G

R

S

W

W

S

S

E

D

Q

Q

E

D

I

L

V

F

D

Q

Y

Y

L

L

K

K

T

T

G

G

R

K

N

T

A

A

H

H

S

A

V
x
R

V

A

G

G

T
x
P

M
|
M

A

A

E

E

V

A

F

I

N

E

N

H

S

S

T

L

Q

Q

Y

Y

M

L

S

P

D

D

P

A

D

D

L

I

K

S

A

A

I

I

A

V

H

T

Y

Y

L

L

V

R

S

S

L

V

P

P

G

A

D

K

P

A

K

E

-

S

-

G

R

Q

D

T

G

V

P

A

P

N

W

F

K

E

P

H

A

A

A

G

K

R

L

P

A

S

E

S

Q

Y

P

S

L

V

S

T

T

T

P

D

G

G

A

A

N

L

Y

Y

M

E

A

A

K
x
V

C
|
C

S

A

S

S

C
|
C

H
|
H

G

Q

S

S

D

D

G

G

S

K

G

G

Q

S

A

K

P

D

-

G

W
x
Y

I
x
Y

P
|
P

P

S

L
|
L

A

V

G

G

A
x
N

S
x
T

S

T

M

G

V

Q

K

L

E

N

G

P

A

N

T
x
D

S

L

I

I

N

A

A

S

T
x
I

L

L

N

Y

G

G

S
x
V

E

D

R
|
R

V

T

A

D

N

N

G

H

I

E

-
x
I

-

L

-
x
M

-
x
P

-

A

-
x
F

-

G

P

P

D

D

S

S

Y

L

R

V

M

Q

P

P

P

-

Y

-

R

-

N

-

Q

-

L

L

S

T

D

D

Q

E

E

Q

V

I

A

A

D

T

V

I

L

A

T

D

F

Y

V

V

R

L

T

S

S

H

W

F

G

G

N

N

Q

A

G

Q

G

A

A

T

V

V

K

S

A

A

D

D

E

A

V

V

K

K

E

Q

L

V

R

R

binding heme c: C13 (= C57), C16 (= C60), H17 (= H61), T42 (= T85), I44 (= I87), Y55 (= Y98), L75 (= L118), Y76 (= Y119), A78 (= A121), M79 (= M122), R122 (= R165), H161 (= H203), C162 (= C204), C165 (= C207), H166 (= H208), A191 (= A238), P192 (= P239), R223 (≠ V270), P227 (≠ T274), M228 (= M275), V289 (≠ K334), C290 (= C335), C293 (= C338), H294 (= H339), Y305 (≠ W349), Y306 (≠ I350), P307 (= P351), L309 (= L353), N312 (≠ A356), T313 (≠ S357), T314 (≠ S358), D322 (≠ T366), I327 (≠ T371), V331 (≠ S375), R333 (= R377), I340 (vs. gap), M342 (vs. gap), P343 (vs. gap), F345 (vs. gap)

8jejC Cryo-em structure of na-dithionite reduced membrane-bound fructose dehydrogenase from gluconobacter japonicus
38% identity, 86% coverage: 45:428/444 of query aligns to 1:400/413 of 8jejC

query
sites
8jejC

V

I

Q

S

R

R

G

G

E

H

Y

Y

V

L

A

A

R

I

L

A

S

A

D

D

C
|
C

V

A

A

A

C
|
C

H
|
H

S

T

T

N

P

G

K

R

G

D

A

G

P

Q

F

F

-

L

A

A

G

G

L

Y

E

A

M

I

A

S

T

S

P

P

M
|
M

G

G

S

N

I

I

Y

Y

A

S

T
|
T

N

N

I
|
I

T

T

P

P

D

S

K

K

Q

T

T

H

G

G

I

I

G

G

N

N

Y

Y

S

T

L

L

A

E

D

Q

F
|
F

D

S

R

K

A

A

V
x
L

R

R

S

H

G

G

V

I

A

R

A

A

D

D

G

G

H

A

R

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
|
M

P
|
P

Y

Y

P

D

S

A

Y
|
Y

A

N

K

R

L

L

S

T

D

D

D

E

D

D

V

V

R

K

A

S

L

L

Y

Y

A

A

F

Y

F

I

M

M

A

T

G

E

V

V

K

K

P

P

A

V

Q

D

Q

A

Q

P

N

S

Q

P

Q

K

S

T

H

Q

I

L

P

P

W

F

P

P

L

F

N

S

M

I

R
|
R

W

A

P
x
S

L

L

A

G

L

I

W
|
W

N

K

T

I

A

A

F

A

V

R

D

I

D

E

G

G

A

K

-

P

Y

Y

Q

V

A

F

K

D

P

H

S

T

E

H

D

N

A

D

L

D

W

W

N

N

R

R

G

G

A

R

Y

Y

I

L

V

V

Q

D

G

E

A

L

G

A

H

H

C
|
C

G

G

S

E

C
|
C

H
|
H

T

T

P

P

R

R

S

N

L

F

T

L

M

-

N

-

E

-

K

-

S

-

L
|
L

D

A

E

P

S

N

S

Q

A

S

T

A

F

Y

L

L

S

A

G

G

S

A

L

D

L
x
I

D

G

G

S

W
|
W

Y

R

A
|
A

P
|
P

S

N

L
x
I

R

T

Q

N

D

A

P

P

N

Q

T

S

G

G

L

I

G

G

R

S

W
|
W

S

S

E

D

Q

Q

E

D

I

L

V

F

D

Q

Y
|
Y

L

L

K

K

T

T

G

G

R

K

N

T

A

A

H

H

S

A

V
x
R

V

A

G

G

T

P

M
|
M

A

A

E

E

V

A

F

I

N

E

N

H

S

S

T

L

Q

Q

Y

Y

M

L

S

P

D

D

P

A

D

D

L

I

K

S

A

A

I

I

A

V

H

T

Y

Y

L

L

V

R

S

S

L

V

P

P

G

A

D

K

P

A

-

E

-

S

-

G

-

Q

-

T

-

V

-

A

-

N

-

F

K

E

R

H

D

A

G

G

P

R

P

P

W

S

K

S

P

Y

A

S

A

V

K

A

L

N

A

A

E

N

Q

S

P

R

L

R

S

S

-

N

-

S

-

T

-

L

-

T

-

K

-

T

T

T

P

D

G

G

A

A

N

L

Y

Y

M

E

A

A

K
x
V

C
|
C

S

A

S

S

C
|
C

H
|
H

G

Q

S

S

D

D

G

G

S

K

G

G

Q

S

A

K

P

D

-

G

W

Y

I
x
Y

P
|
P

P

S

L
|
L

A

V

G

G

A

N

S
x
T

S

T

M

G

V

Q

K

L

E

N

G

P

A

N

T
x
D

S

L

I

I

N

A

A

S

T
x
I

L

L

N

Y

G

G

S
x
V

E

D

R
|
R

V

T

A

D

N

N

G

H

I

E

-
x
I

-

L

-
x
M

-

P

-

A

-
x
F

-

G

P

P

D

D

S

S

Y

L

R

V

M

Q

P

P

P

-

Y

-

R

-

N

-

Q

-

L

L

S

T

D

D

Q

E

E

Q

V

I

A

A

D

T

V
x
I

L

A

T

D

F

Y

V

V

R

L

T

S

S

H

W

F

G

G

N

N

Q

A

G

Q

G

A

A

T

V

V

K

S

A

A

D

D

E

A

V

V

K

K

E

Q

L

V

R

R

binding heme c: C13 (= C57), C16 (= C60), H17 (= H61), T42 (= T85), I44 (= I87), F60 (= F103), L64 (≠ V107), L75 (= L118), Y76 (= Y119), M79 (= M122), P80 (= P123), Y84 (= Y127), R122 (= R165), C162 (= C204), C165 (= C207), H166 (= H208), I186 (≠ L233), W189 (= W236), A191 (= A238), P192 (= P239), I194 (≠ L241), W205 (= W252), Y213 (= Y260), R223 (≠ V270), M228 (= M275), V303 (≠ K334), C304 (= C335), C307 (= C338), H308 (= H339), Y320 (≠ I350), P321 (= P351), L323 (= L353), T327 (≠ S357), T328 (≠ S358), D336 (≠ T366), I341 (≠ T371), V345 (≠ S375), R347 (= R377), I354 (vs. gap), M356 (vs. gap), F359 (vs. gap), I376 (≠ V404)
binding ubiquinone-10: M36 (= M79), P77 (= P120), S124 (≠ P167), W128 (= W171), C165 (= C207), L173 (= L220)

Sites not aligning to the query:

binding ubiquinone-10: 408, 411

8gy3A Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
36% identity, 85% coverage: 35:411/444 of query aligns to 33:410/440 of 8gy3A

query
sites
8gy3A

A

A

S

S

A

S

P

F

S

S

A

A

D

D

A

A

Q

-

L

-

V

I

Q

S

R

R

G

G

E

E

Y

I

V

V

A

A

R

N

L

G

S

G

D
x
Y

C
|
C

V

A

A
x
E

C
|
C

H
|
H

S

T

T

R

P

V

K

D

G

G

A

K

P

P

-

G

-

P

-

E

F

L

A

A

G

G

G

D

L

F

E

K

M

M

A

A

T
|
T

P

P

M
x
F

G

G

S

D

I

I

Y

F

A

S

T
x
S

N

N

I
|
I

T

T

P

P

D

D

K

E

Q

E

T

W

G

G

I

I

G

G

N

N

Y
x
W

S

S

L

L

A

A

D

A

F
|
F

D

K

R

R

A

A

V

M

R

N

S

K

G

G

V

I

A

A

A

R

D

D

G

G

H

S

R

Q

L
|
L

Y
|
Y

P
|
P

A

A

M
x
F

P

P

Y

F

P

D

S

H

Y
x
F

A

T

K

K

L

V

S

S

D

D

D

Q

D

D

V

V

R

S

A

D

L

L

Y

Y

A

A

F

Y

F

L

M

M

A

-

G

-

V

T

K

R

P

P

A

A

-

V

Q

H

Q

L

Q

K

N

P

Q

R

Q

D

S

N

H

T

I

V

P

P

W

F

P

P

L
x
I

N

N

M

I

R
|
R

W

L

P

I

-
x
G

L
x
Q

A

G

L
x
F

W
|
W

N

K

T

L

A

L

F

F

V

F

D

T

D

P

G

G

A

R

Y

Y

Q

Q

A

N

K

D

P

P

S

K

E

H

D

D

A

A

L

Q

W

W

N

N

R

R

G

G

A

A

Y

Y

I

L

V

A

Q

E

G

G

A

N

G

E

H
|
H

C
|
C

G

G

S

A

C
|
C

H
|
H

T

T

P

P

R
|
R

S

N

L

L

T
x
L

M

G

N

A

E

E

K

K

S

-

L

-

D

-

E

-

S

-

S

M

A

S

T

S

F

V

L

Y

S

D

G

G

S

A

L

V

L

I

D

D

G

G

W

W

Y

I

A
|
A

P
|
P

S

P

L
|
L

R

-

Q

N

D

D

P

H

N

N

T

P

G

T

L

P

G

V

R

V

W
|
W

S

T

E

E

Q

D

E

E

I

L

V

F

D

Q

Y
|
Y

L
|
L

K

R

T

F

G

G

R

V

N

A

-

P

A

L

H

H

S
x
G

V
x
S

V

A

G

G

T

P

M
|
M

A

S

E

P

V

V

F

P

N

H

N

R

S

F

T

L

Q

S

Y

K

M

I

S

P

D

E

P

E

D

D

L

V

K

H

A

A

I

I

A

A

H

H

Y

Y

L
x
Y

V

A

S

D

L

V

P

D

G

K

D

A

P

A

K

Q

R

R

-

S

-

G

D

D

G

Q

P

A

W

I

K

T

P

R

A

A

A

M

K

Q

L

M

A

S

E

G

Q

R

P

D

L

L

S

T

T

G

P

P

-

Q

-

P

-

L

-

D

-

E

G

D

A

A

A

R

N

L

Y

Y

M

Q

A

G

K

A

C
|
C

S

G

S

A

C
|
C

H
|
H

G

Y

S

N

D

S

G

G

S

P

G

N

Q

P

A

V

P

L

W

G

I

R

P
|
P

P

E

L
|
L

A

A

G

L

A
x
N

S
x
N

S

A

S

L

M

W

V

L

K

D

E

E

G

P

A

N

T

N

S

L

I

Y

N

Q

A
x
V

T

M

L

L

N

H

G

G

S

-

E

-

R

-

V
x
I

V

T

A

A

N

E

G

E

I
x
G

P
x
Q

D

D

S

H

Y
x
I

R

S

M
|
M

P

P

P

S

Y
x
F

R

Y

N

S

Q

G

L

L

S

S

D

D

Q

H

E

D

V

M

A

A

D

R

V

I

L

A

T

A

F

Y

V

L

R

R

T

R

S

T

binding heme c: Y52 (≠ D56), C53 (= C57), C56 (= C60), H57 (= H61), S84 (≠ T85), I86 (= I87), W97 (≠ Y98), F102 (= F103), L117 (= L118), F121 (≠ M122), F126 (≠ Y127), R163 (= R165), C203 (= C204), C206 (= C207), H207 (= H208), A232 (= A238), P233 (= P239), L235 (= L241), W245 (= W252), Y253 (= Y260), L254 (= L261), G263 (≠ S269), S264 (≠ V270), M269 (= M275), Y292 (≠ L298), C337 (= C335), C340 (= C338), H341 (= H339), P353 (= P351), L355 (= L353), N358 (≠ A356), N359 (≠ S357), V372 (≠ A370), I377 (≠ V378), G382 (≠ I383), Q383 (≠ P384), I386 (≠ Y387), M388 (= M389), F391 (≠ Y392)
binding ubiquinone-10: E55 (≠ A59), T76 (= T77), F78 (≠ M79), Y118 (= Y119), P119 (= P120), I160 (≠ L162), G166 (vs. gap), Q167 (≠ L168), F169 (≠ L170), W170 (= W171), H202 (= H203), R210 (= R211), L213 (≠ T214)

6fjaA Crystal structure of t2d three-domain heme-cu nitrite reductase from ralstonia pickettii
38% identity, 23% coverage: 327:430/444 of query aligns to 352:454/455 of 6fjaA

query
sites
6fjaA

G

G

A

R

A

A

N

L

Y

F

M

A

A

G

K
x
T

C
|
C

S

S

S

V

C
|
C

H
|
H

G

Q

S

G

D

N

G

G

S

A

G

G

Q

L

A

P

P

G

W

V

I

F

P
|
P

L
|
L

A

A

G

-

A

K

S

S

S

D

S
x
F

M

L

V

A

K

A

E

D

G

P

A

K

T

R

S

A

I

M

N

N

A

I

T

V

L

L

N

H

G

G

-

L

S
x
N

E
x
G

R

K

V

I

V

K

A

V

N

N

G

G

I

Q

P

E

D
x
Y

S

D

Y
x
S

R

V

M
|
M

P

P

Y

-

R
x
M

N

T

Q

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

F

Y

V

V

R

L

T

N

S

S

W

W

G

D

N

N

Q

P

G

G

A

R

V

V

K

S

A

A

D

E

E

D

V

V

K

K

E

K

L

V

R

R

E

A

R

Q

binding protoporphyrin ix containing fe: T359 (≠ K334), C360 (= C335), C363 (= C338), H364 (= H339), P376 (= P351), P377 (= P352), L378 (= L353), F383 (≠ S359), N400 (≠ S375), G401 (≠ E376), Y410 (≠ D385), S412 (≠ Y387), M414 (= M389), M417 (≠ R393)

Sites not aligning to the query:

active site: 90, 93, 95, 130, 131, 139, 144, 236, 258, 259, 285
binding copper (ii) ion: 90, 131, 139, 144

5oboA Crystal structure of nitrite bound d97n mutant of three-domain heme-cu nitrite reductase from ralstonia pickettii
38% identity, 23% coverage: 327:430/444 of query aligns to 353:455/456 of 5oboA

query
sites
5oboA

G

G

A

R

A

A

N

L

Y

F

M

A

A

G

K
x
T

C
|
C

S

S

S

V

C
|
C

H
|
H

G

Q

S

G

D

N

G

G

S

A

G

G

Q

L

A

P

P

G

W

V

I

F

P
|
P

L
|
L

A

A

G

-

A

K

S
|
S

S

D

S
x
F

M

L

V

A

K

A

E

D

G

P

A

K

T

R

S

A

I

M

N

N

A
x
I

T

V

L

L

N

H

G

G

-

L

S
x
N

E
x
G

R

K

V

I

V

K

A

V

N

N

G

G

I

Q

P

E

D

Y

S

D

Y
x
S

R

V

M
|
M

P

P

Y

-

R
x
M

N

T

Q

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

F

Y

V

V

R

L

T

N

S

S

W

W

G

D

N

N

Q

P

G

G

A

R

V

V

K

S

A

A

D

E

E

D

V

V

K

K

E

K

L

V

R

R

E

A

R

Q

binding heme c: T360 (≠ K334), C361 (= C335), C364 (= C338), H365 (= H339), P377 (= P351), P378 (= P352), L379 (= L353), S382 (= S357), F384 (≠ S359), I395 (≠ A370), N401 (≠ S375), G402 (≠ E376), S413 (≠ Y387), M415 (= M389), M418 (≠ R393)

Sites not aligning to the query:

active site: 91, 94, 96, 131, 132, 140, 145, 237, 259, 260, 286
binding copper (ii) ion: 91, 96, 131, 132, 140, 145
binding nitrite ion: 94, 96, 131

4ax3D Structure of three-domain heme-cu nitrite reductase from ralstonia pickettii at 1.6 a resolution (see paper)
38% identity, 23% coverage: 327:430/444 of query aligns to 355:457/457 of 4ax3D

query
sites
4ax3D

G

G

A

R

A

A

N

L

Y

F

M

A

A

G

K

T

C
|
C

S

S

S

V

C
|
C

H
|
H

G

Q

S

G

D

N

G

G

S

A

G

G

Q

L

A

P

P

G

W

V

I

F

P
|
P

L
|
L

A

A

G

-

A

K

S
|
S

S

D

S
x
F

M

L

V

A

K

A

E

D

G

P

A

K

T

R

S

A

I

M

N

N

A

I

T

V

L

L

N

H

G

G

-

L

S
x
N

E
x
G

R

K

V

I

V

K

A

V

N

N

G

G

I

Q

P

E

D

Y

S

D

Y
x
S

R

V

M
|
M

P

P

Y

-

R
x
M

N

T

Q

Q

L

L

S

N

D

D

Q

D

E

E

V

V

A

A

D

N

V

I

L

L

T

T

F

Y

V

V

R

L

T

N

S

S

W

W

G

D

N

N

Q

P

G

G

A

R

V

V

K

S

A

A

D

E

E

D

V

V

K

K

E

K

L

V

R

R

E

A

R

Q

binding heme c: C363 (= C335), C366 (= C338), H367 (= H339), P379 (= P351), P380 (= P352), L381 (= L353), S384 (= S357), F386 (≠ S359), N403 (≠ S375), G404 (≠ E376), S415 (≠ Y387), M417 (= M389), M420 (≠ R393)

Sites not aligning to the query:

active site: 93, 96, 98, 133, 134, 142, 147, 239, 261, 262, 288
binding copper (ii) ion: 93, 98, 133, 134, 142, 147

2zooA Crystal structure of nitrite reductase from pseudoalteromonas haloplanktis tac125
34% identity, 22% coverage: 331:428/444 of query aligns to 343:438/438 of 2zooA

query
sites
2zooA

Y

Y

M

E

A

A

K

N

C
|
C

S

M

S

A

C
|
C

H
|
H

G

Q

S

A

D

N

G

G

S

E

G

G

Q

I

A

P

P

G

W

A

I
x
F

P
|
P

L
|
L

A

A

G

-

A

K

S
|
S

S

D

S
x
Y

M

L

V

N

K

N

E

N

G

P

A

L

T

L

S

G

I

V

N

N

A

A

T
x
I

L

I

N

K

G

G

-
x
L

S
|
S

E
x
G

R

P

V
x
I

V

K

A
x
V

N

N

G

N

I

V

P

N

D
x
Y

S
x
N

Y
x
G

R
x
V

M
|
M

P

P

P

A

Y

M

R

-

N

-

Q

N

L

L

S

N

D

D

Q

E

E

D

V

I

A

A

D

N

V

V

L

I

T

T

F

F

V

V

R

L

T

N

S

N

W

W

G

D

N

N

Q

A

G

G

A

K

V

V

K

S

A

A

D

E

E

Q

V

V

K

A

E

K

L

Q

R

R

binding protoporphyrin ix containing fe: C347 (= C335), C350 (= C338), H351 (= H339), F362 (≠ I350), P363 (= P351), P364 (= P352), L365 (= L353), S368 (= S357), Y370 (≠ S359), I382 (≠ T371), L386 (vs. gap), S387 (= S375), G388 (≠ E376), I390 (≠ V378), V392 (≠ A380), Y397 (≠ D385), N398 (≠ S386), G399 (≠ Y387), V400 (≠ R388), M401 (= M389)

Sites not aligning to the query:

active site: 81, 84, 86, 121, 122, 130, 135, 227, 249, 250, 276
binding copper (ii) ion: 81, 86, 121, 122, 130, 135

8hddB Complex structure of catalytic, small, and a partial electron transfer subunits from burkholderia cepacia fad glucose dehydrogenase
27% identity, 27% coverage: 309:428/444 of query aligns to 1:121/121 of 8hddB

query
sites
8hddB

G

G

P

K

P

P

W

A

K

E

P

D

A

G

A

L

K

K

L

L

A

R

E

G

Q

V

P

A

L

L

S

A

T

S

P

S

G

G

-

I

-

D

-

P

A

A

A

R

N

L

Y

Y

M

L

A

G

K

N

C
|
C

S

A

S

T

C
|
C

H
|
H

G

Q

S

M

D

Q

G

G

S

K

G

G

Q

T

A

P

P

D

-

G

W

Y

I
x
Y

P
|
P

P

S

L

L

A

F

G

H

A

N

S

S

S
x
T

S

V

M

G

V

A

K

S

E

N

G

P

A

S

T

N

S

L

I

V

N

Q

A
x
V

T
x
I

L

L

N

N

G

G

S

V

E

Q

R
|
R

V

K

V

I

A

G

N

S

G

-

I

-

P

-

D

E

S

D

Y
x
I

R

G

M
|
M

P
|
P

P

A

Y

F

R

R

N

Y

Q

D

L

L

S

N

D

D

Q

A

E

Q

V

I

A

A

D

A

V

L

L

T

T

N

F

Y

V

V

R

T

T

A

S

Q

W

F

G

G

N

N

Q

P

G

A

A

K

V

V

K

T

A

E

D

Q

E

D

V

V

K

A

E

K

L

L

R

R

binding protoporphyrin ix containing fe: C30 (= C335), C33 (= C338), H34 (= H339), Y46 (≠ I350), P47 (= P351), T54 (≠ S358), V66 (≠ A370), I67 (≠ T371), R73 (= R377), I80 (≠ Y387), M82 (= M389), P83 (= P390)

1dt1A Thermus thermophilus cytochrome c552 synthesized by escherichia coli (see paper)
32% identity, 23% coverage: 328:430/444 of query aligns to 2:112/129 of 1dt1A

query
sites
1dt1A

A

G

A

A

N

K

Y

I

M

Y

A

A

K

Q

C
|
C

S

A

S

G

C
|
C

H
|
H

G

Q

S

Q

D

N

G

G

S

Q

G

G

Q

I

A

P

P

G

W

A

I

F

P
|
P

P

P

L

L

A

A

G

G

-
x
H

A

V

S

A

S

E

S

I

M

L

V

A

K

K

E

E

G

G

A

G

T

R

S

E

-
x
Y

-

L

I

I

N

L

A
x
V

T

L

L

L

N

Y

G

G

S

L

E
x
Q

R
x
G

V

Q

V

I

A

E

-

V

-

K

-

G

-

M

-

K

-

Y

N

N

G
|
G

I

V

P
x
M

D

S

S

S

Y
x
F

R

A

M

-

P

-

Y

-

R

-

N

-

Q

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

F

H

V

I

R

A

T

T

S

A

W

W

G

G

N

D

Q

A

G

K

A

V

-

K

-

G

-

F

-

K

-

P

V

F

K

T

A

A

D

E

E

E

V

V

K

K

E

K

L

L

R

R

E

A

R

K

binding heme c: C9 (= C335), C12 (= C338), H13 (= H339), P25 (= P351), H30 (vs. gap), Y43 (vs. gap), V47 (≠ A370), Q53 (≠ E376), G54 (≠ R377), G65 (= G382), M67 (≠ P384), F70 (≠ Y387)

Sites not aligning to the query:

binding heme c: 123

1r0qA Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (spirographis) heme (see paper)
32% identity, 23% coverage: 328:430/444 of query aligns to 3:113/130 of 1r0qA

query
sites
1r0qA

A

G

A

A

N

K

Y

I

M

Y

A

A

K

Q

C

C

S

A

S

G

C
|
C

H
|
H

G

Q

S

Q

D

N

G

G

S

Q

G

G

Q

I

A

P

P

G

W

A

I

F

P
|
P

P

P

L
|
L

A

A

G

G

-
x
H

A

V

S

A

S

E

S

I

M

L

V

A

K

K

E

E

G

G

A

G

T

R

S

E

-
x
Y

-

L

I

I

N

L

A
x
V

T

L

L

L

N

Y

G

G

S

L

E
x
Q

R
x
G

V

Q

V

I

A

E

-

V

-

K

-

G

-

M

-

K

-

Y

N

N

G
|
G

I

V

P
x
M

D

S

S

S

Y

F

R

A

M

-

P

-

Y

-

R

-

N

-

Q

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

F

H

V

I

R

A

T

T

S

A

W

W

G

G

N

D

Q

A

G

K

A

V

-

K

-

G

-

F

-

K

-

P

V

F

K

T

A

A

D

E

E

E

V

V

K

K

E

K

L

L

R

R

E

A

R

K

binding 2-formyl-protoporphryn ix: C13 (= C338), H14 (= H339), P26 (= P351), L28 (= L353), H31 (vs. gap), Y44 (vs. gap), V48 (≠ A370), Q54 (≠ E376), G55 (≠ R377), G66 (= G382), M68 (≠ P384)

Sites not aligning to the query:

binding 2-formyl-protoporphryn ix: 124

1qyzA Characterization of the malformed, recombinant cytochrome rc552 (see paper)
32% identity, 23% coverage: 328:430/444 of query aligns to 3:113/130 of 1qyzA

query
sites
1qyzA

A

G

A

A

N

K

Y

I

M
x
Y

A

A

K

Q

C
|
C

S

A

S

G

C
|
C

H
|
H

G

Q

S

Q

D

N

G

G

S

Q

G

G

Q

I

A

P

P

G

W

A

I

F

P
|
P

P

P

L

L

A

A

G

G

-
x
H

A

V

S

A

S

E

S

I

M

L

V

A

K

K

E

E

G

G

A

G

T

R

S

E

-
x
Y

-

L

I

I

N

L

A

V

T

L

L

L

N

Y

G

G

S

L

E
x
Q

R
x
G

V

Q

V

I

A

E

-

V

-

K

-

G

-

M

-

K

-

Y

N

N

G
|
G

I

V

P
x
M

D

S

S

S

Y
x
F

R

A

M

-

P

-

Y

-

R

-

N

-

Q

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

F

H

V

I

R

A

T

T

S

A

W

W

G

G

N

D

Q

A

G

K

A

V

-

K

-

G

-

F

-

K

-

P

V

F

K

T

A

A

D

E

E

E

V

V

K

K

E

K

L

L

R

R

E

A

R

K

binding 2-acetyl-protoporphyrin ix: Y7 (≠ M332), C10 (= C335), C13 (= C338), H14 (= H339), P26 (= P351), H31 (vs. gap), Y44 (vs. gap), Q54 (≠ E376), G55 (≠ R377), G66 (= G382), M68 (≠ P384), F71 (≠ Y387)

Sites not aligning to the query:

binding 2-acetyl-protoporphyrin ix: 124

1c52A Thermus thermophilus cytochrome-c552: a new highly thermostable cytochromE-C structure obtained by mad phasing (see paper)
32% identity, 23% coverage: 328:430/444 of query aligns to 4:114/131 of 1c52A

query
sites
1c52A

A

G

A

A

N

K

Y

I

M

Y

A

A

K

Q

C
|
C

S

A

S

G

C
|
C

H
|
H

G

Q

S

Q

D

N

G

G

S

Q

G

G

Q

I

A

P

P

G

W

A

I
x
F

P
|
P

P

P

L
|
L

A

A

G

G

-
x
H

A

V

S

A

S

E

S

I

M

L

V

A

K

K

E

E

G

G

A

G

T

R

S

E

-
x
Y

-

L

I

I

N

L

A

V

T

L

L

L

N

Y

G

G

S
x
L

E
x
Q

R
x
G

V

Q

V

I

A

E

-

V

-

K

-

G

-

M

-

K

-

Y

N

N

G
|
G

I

V

P
x
M

D

S

S

S

Y
x
F

R

A

M

-

P

-

Y

-

R

-

N

-

Q

Q

L

L

S

K

D

D

Q

E

E

E

V

I

A

A

D

A

V

V

L

L

T

N

F

H

V

I

R

A

T

T

S

A

W

W

G

G

N

D

Q

A

G

K

A

V

-

K

-

G

-

F

-

K

-

P

V

F

K

T

A

A

D

E

E

E

V

V

K

K

E

K

L

L

R

R

E

A

R

K

binding protoporphyrin ix containing fe: C11 (= C335), C14 (= C338), H15 (= H339), F26 (≠ I350), P27 (= P351), L29 (= L353), H32 (vs. gap), Y45 (vs. gap), L54 (≠ S375), Q55 (≠ E376), G56 (≠ R377), G67 (= G382), M69 (≠ P384), F72 (≠ Y387)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 125

Query Sequence

>Pf6N2E2_1903 Putative diheme cytochrome c-553
VNKTIKYLLPAGIVVAAGLGWFVNRTPFSPFANEASAPSADAQLVQRGEYVARLSDCVAC
HSTPKGAPFAGGLEMATPMGSIYATNITPDKQTGIGNYSLADFDRAVRSGVAADGHRLYP
AMPYPSYAKLSDDDVRALYAFFMAGVKPAQQQNQQSHIPWPLNMRWPLALWNTAFVDDGA
YQAKPSEDALWNRGAYIVQGAGHCGSCHTPRSLTMNEKSLDESSATFLSGSLLDGWYAPS
LRQDPNTGLGRWSEQEIVDYLKTGRNAHSVVVGTMAEVFNNSTQYMSDPDLKAIAHYLVS
LPGDPKRDGPPWKPAAKLAEQPLSTPGAANYMAKCSSCHGSDGSGQAPWIPPLAGASSSM
VKEGATSINATLNGSERVVANGIPDSYRMPPYRNQLSDQEVADVLTFVRTSWGNQGGAVK
ADEVKELRERTNPASSNPIILQMR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory